Canadian Patents Database / Patent 2737704 Summary

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(12) Patent: (11) CA 2737704
(54) English Title: MICROBIAL GROWTH STIMULANTS FROM PLANT BIOMASS AND METHODS RELATED THERETO
(54) French Title: STIMULATEURS DE CROISSANCE MICROBIENS ISSUS DE LA BIOMASSE ET PROCEDES CONNEXES
(51) International Patent Classification (IPC):
  • C12N 9/00 (2006.01)
  • C10L 1/02 (2006.01)
  • C12M 1/00 (2006.01)
  • C12N 1/14 (2006.01)
  • C12N 1/20 (2006.01)
  • C12N 1/38 (2006.01)
  • C12N 9/24 (2006.01)
  • C12N 9/42 (2006.01)
  • C12P 1/00 (2006.01)
  • C12P 7/10 (2006.01)
  • C12P 19/14 (2006.01)
(72) Inventors (Country):
  • LAU, MING WOEI (United States of America)
  • DALE, BRUCE (United States of America)
  • BALAN, VENKATESH (United States of America)
  • CHUNDAWAT, SHISHIR (United States of America)
(73) Owners (Country):
  • BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY (United States of America)
(71) Applicants (Country):
  • BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY (United States of America)
(74) Agent: RIDOUT & MAYBEE LLP
(45) Issued: 2013-11-19
(22) Filed Date: 2011-04-19
(41) Open to Public Inspection: 2011-10-19
Examination requested: 2011-04-19
(30) Availability of licence: N/A
(30) Language of filing: English

(30) Application Priority Data:
Application No. Country Date
12/763,102 United States of America 2010-04-19

English Abstract

Methods for treating plant biomass with ammonia to provide an ammonia- pretreated plant biomass and using the ammonia-pretreated plant biomass to stimulate growth or promote enzyme synthesis in an enzyme-producing microorganism are provided. The process can economically and efficiently be performed in a bio- product production facility.


French Abstract

Méthodes de traitement de la phytomasse avec de l'ammoniac afin de fournir une phytomasse prétraitée à l'ammoniac et d'utiliser la phytomasse prétraitée à l'ammoniac pour stimuler la croissance ou faciliter la synthèse d'une enzyme dans un microorganisme producteur d'enzyme. Le processus peut être effectué, de façon économique et efficace, dans une installation de production de bioproduits.


Note: Claims are shown in the official language in which they were submitted.

WHAT IS CLAIMED IS:
1. A method of producing and using a stimulant comprising:
treating plant biomass with an ammonia pretreatment to provide an ammonia-
pretreated plant biomass, wherein the plant biomass is from one or more plants
and
contains solubles;
extracting at least a portion of the solubles from the ammonia-pretreated
plant
biomass with an extractive additive to produce a soluble extract and an
extracted
ammonia-pretreated plant biomass, wherein the extractive additive comprises
water;
and
using the soluble extract as the stimulant to stimulate growth and/or promote
cellulase and/or hemicellulase enzyme synthesis in an enzyme-producing
microorganism.
2. The method of claim 1, wherein the method is performed in a bio-product
production facility that utilizes the ammonia-pretreated plant biomass to
produce a
bio-product in a bio-product production unit.
3. The method of claim 2, wherein the enzyme-producing microorganism is
contained within an enzyme production unit that optionally comprises the
soluble
extract, the extracted ammonia-pretreated biomass and/or a nutrient-containing

byproduct of the bio-product production unit.
4. The method of claim 3, wherein the enzyme-producing microorganism
produces a saccharolytic enzyme.
5. The method of claim 4, wherein the saccharolytic enzyme is selected from

exoglucanases, endoglucanases, .beta.-xylosidases, endoxylanases, .alpha.-
arabinofuranosidases, cellulose induced protein 2, .alpha.-
arabinofuranosidases, acetyl
xylan esterases, .alpha.-glucuronidases, endoxylanases, polygalaturonases,
.alpha.-
galactosidases, acetyl esterases, and combinations thereof
6. The method of claim 2, wherein the enzyme-producing microorganism is
selected from yeast, bacteria, fungi, and combinations thereof
53

7. The method of claim 6, wherein the enzyme-producing microorganism is
selected from Trichoderma reesei, Aspergillus Awamori, Clostridium
thermocellum
and Thermoanaerobacterium saccharolyticum.
8. The method of claim 1, wherein the soluble extract comprises proteins,
vitamins, simple sugars, oligosaccharides, lipids, trace elements or
combinations
thereof
9. The method of claim 1, wherein the extractive additive further includes
an
aqueous alkaline ammonium hydroxide solution.
10. The method of claim 9, wherein the aqueous alkaline ammonium hydroxide
solution comprises about 0.01% to about 30% NH4OH by weight.
11. The method of claim 10, wherein the aqueous alkaline ammonium hydroxide

solution has a pH between about 7 and about 12.
12. The method of claim 1, wherein at least one of the one or more plants
is a
monocot.
13. The method of claim 12, wherein the monocot is grass, corn stover,
sorghum,
sugarcane bagasse, wheat, rice, maize, or a combination thereof
14. The method of claim 13, wherein the grass is switchgrass, miscanthus,
reed
canary grass, or a combination thereof
15. The method of claim 2, wherein the plant biomass is corn stover.
16. The method of claim 2, wherein the ammonia pretreatment comprises
ammonia fiber expansion.
54

17. The method of claim 2, wherein a portion of the extracted ammonia-
pretreated
plant biomass is also provided to the bio-product production unit and the
method
further comprises hydrolyzing said extracted ammonia-pretreated plant biomass
and
the ammonia pre-treated biomass with an enzyme to generate an enzymatic
hydrolysate slurry, wherein the enzyme is an enzyme secreted by the enzyme-
producing microorganism.
18. The method of claim 17, further comprising mechanically processing the
enzymatic hydrolysate slurry to produce a liquid enzymatic hydrolysate
comprising a
fermentable sugar mixture.
19. The method of claim 18, further comprising fermenting the liquid
enzymatic
hydrolysate to generate a bio-product.
20. The method of claim 17, wherein the bio-product production unit is part
of a
biofuel production facility and is configured to produce a bio-product and a
nutrient-
containing byproduct from the extracted ammonia-pretreated plant biomass
and/or the
ammonia-pretreated plant biomass, wherein the bio-product production facility
further
comprises an enzyme production unit in communication with the bio-product
production unit, the enzyme production unit configured to produce the enzyme
from
the soluble extract for use in the bio-product production unit.
21. The method of claim 20, wherein the bio-product is a biofuel or
biochemical.
22. The method of claim 21, wherein the biofuel is ethanol and the ammonia-
pretreated plant biomass is ammonia pretreated corn stover.
23. The method of any one of claims 17 to 20 wherein the enzyme is produced
in
an enzyme production unit which comprises the enzyme-producing microorganism,
an ethanol production residue, and the stimulant.
24. The method of any one of claims 1 to 23, wherein the ammonia-pretreated

plant biomass is ammonia treated corn stover.

25. The method of claim 23, wherein the ethanol production residue is a
nutrient-
containing bioproduct generated in the bio-product production unit.
26. The method of claim 25, wherein the ethanol production residue is corn
syrup
liquor.
27. The method of claim 25, where the enzyme production unit further
comprises
a diluted pretreated biomass hydrolysate.
28. The method of claim 25, where the enzyme-producing microorganism is
selected from Trichoderma reesei, Aspergillus Awamori, Clostridium
thermocellum
and Thermoanaerobacterium saccharolyticum.
29. The method of claim 25, where the enzyme produced is a saccharolytic
enzyme.
30. The method of claim 29, wherein the saccharolytic enzyme is selected
from
exoglucanases, endoglucanases, .beta.-xylosidases, endoxylanases, .alpha.-
arabinofuranosidases, cellulose induced protein 2, .alpha.-
arabinofuranosidases, acetyl
xylan esterases, .alpha.-glucuronidases, endoxylanases, polygalaturonases,
.alpha.-
galactosidases, acetyl esterases, and combinations thereof
31. The method of claim 25 wherein the bio-product is a biochemical or
biofuel.
32. A stimulant comprising:
a water soluble extract from an ammonia-pretreated plant biomass; and
a water extracted ammonia-pretreated plant biomass, wherein the soluble
extract and the extracted ammonia-pretreated plant biomass can stimulate
growth
and/or promote cellulase and/or hemicellulase enzyme synthesis in an enzyme-
producing microorganism.
56

33. The method of any one of claims 2, 3, 15 to 17, 19 or 20 further
comprising
combining the ammonia-pretreated plant biomass with the extracted ammonia-
pretreated plant biomass to form a combined stream prior to providing to the
bio-
product production unit.
34. The method of claim 1, wherein at least one of the one or more plants
is a
dicot.
35. The method of claim 1, wherein a portion of the extracted ammonia-
pretreated
plant biomass is also used as the stimulant.
36. The method of claim 11, wherein the ammonium hydroxide solution is at
least
3% NH4OH by weight.
57


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Admin Status

Title Date
(22) Filed 2011-04-19
Examination Requested 2011-04-19
(41) Open to Public Inspection 2011-10-19
(45) Issued 2013-11-19

Maintenance Fee

Description Date Amount
Last Payment 2016-04-18 $200.00
Next Payment if small entity fee 2017-04-19 $100.00
Next Payment if standard fee 2017-04-19 $200.00

Note : If the full payment has not been received on or before the date indicated, a further fee may be required which may be one of the following

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Payment History

Fee Type Anniversary Year Due Date Amount Paid Paid Date
Request for Examination $800.00 2011-04-19
Filing $400.00 2011-04-19
Registration of Documents $100.00 2011-08-15
Maintenance Fee - Application - New Act 2 2013-04-19 $100.00 2013-04-03
Filing an Amendment after allowance $400.00 2013-07-30
Final $300.00 2013-09-05
Maintenance Fee - Patent - New Act 3 2014-04-22 $100.00 2014-04-14
Maintenance Fee - Patent - New Act 4 2015-04-20 $100.00 2015-04-13
Maintenance Fee - Patent - New Act 5 2016-04-19 $200.00 2016-04-18

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Representative Drawing 2011-09-21 1 13
Cover Page 2011-09-30 2 45
Abstract 2011-04-19 1 10
Description 2011-04-19 52 2,695
Claims 2011-04-19 5 169
Drawings 2011-04-19 19 397
Drawings 2012-08-22 19 394
Claims 2012-08-22 5 166
Description 2012-08-22 52 2,691
Cover Page 2013-10-22 2 47
Drawings 2013-01-30 19 402
Claims 2013-01-30 5 165
Description 2013-01-30 52 2,694
Claims 2013-05-21 5 163
Claims 2013-07-30 5 162
Description 2013-07-30 52 2,695
Representative Drawing 2013-10-22 1 14
Prosecution-Amendment 2012-02-24 1 39
Prosecution-Amendment 2012-02-29 1 20
Prosecution-Amendment 2012-06-04 4 188
Correspondence 2012-08-22 8 253
Prosecution-Amendment 2012-08-22 26 928
Prosecution-Amendment 2012-10-10 2 68
Prosecution-Amendment 2012-11-05 3 115
Prosecution-Amendment 2013-01-30 38 1,584
Prosecution-Amendment 2013-02-21 3 141
Prosecution-Amendment 2013-05-21 12 394
Prosecution-Amendment 2013-07-30 7 241
Correspondence 2013-08-15 1 18
Correspondence 2013-09-05 1 52
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