Canadian Patents Database / Patent 2767844 Summary

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(12) Patent Application: (11) CA 2767844
(54) English Title: TRANSFORMATION OF DICOTYLEDONOUS PLANTS BY AN ISOLATED SHOOT APEX MERISTEM METHOD
(54) French Title: TRANSFORMATION DE PLANTES DICOTYLEDONES PAR UN PROCEDE FAISANT APPEL A UN MERISTEME D'APEX DE TIGE ISOLE
(51) International Patent Classification (IPC):
  • A01H 1/06 (2006.01)
  • C12N 15/82 (2006.01)
(72) Inventors (Country):
  • KOVALCHUK, IGOR (Canada)
  • MAHESHWARI, PRITI (Canada)
(73) Owners (Country):
  • PLANTBIOSIS LTD. (Canada)
(71) Applicants (Country):
  • UNIVERSITY OF LETHBRIDGE (Canada)
(74) Agent: GOWLING WLG (CANADA) LLP
(45) Issued:
(86) PCT Filing Date: 2010-08-12
(87) PCT Publication Date: 2011-02-17
(30) Availability of licence: N/A
(30) Language of filing: English

(30) Application Priority Data:
Application No. Country Date
61/233,213 United States of America 2009-08-12

English Abstract

Method of transforming dicotyledonous plants, the method comprising: (a) isolating a shoot apex from a seedling; (b) dividing the shoot apex into at least two pieces such that the apical meristem is exposed; (c) making an incision into a meristematic region of the apical meristem; (d) exposing the incised meristematic region to a transformation vector and regenerating a plant from the incised shoot apex.


French Abstract

L'invention concerne un procédé de transformation de plantes dicotylédones, qui consiste : (a) à isoler un apex de tige à partir d'un plant; (b) à diviser l'apex de tige en au moins deux morceaux de sorte que le méristème apical soit exposé; (c) à pratiquer une incision dans une zone méristématique du méristème apical; (d) à exposer la zone méristématique incisée à un vecteur de transformation et à régénérer une plante à partir de l'apex de tige incisé.


Note: Claims are shown in the official language in which they were submitted.




CLAIMS

1. A method of transforming dicotyledonous plants, the method comprising:

(a) isolating a shoot apex from a seedling;

(b) dividing the shoot apex into at least two pieces such that the apical
meristem is exposed;

(c) making an incision into a meristematic region of the apical meristem; and
(d) exposing the incised meristematic region to a transformation vector.

2. A method according to claim 1, wherein the division of the shoot apex
exposes
the lateral meristem.

3. A method according to claim 1, wherein the incision penetrates to, and
exposes,
the L2 layer.

4. A method according to claim 1, wherein two incision are made into the
meristomatic region.

5. A method according to claim 1, wherein transformation with the
transformation
vector is mediated by Agrobacterium tumefaciens.

6. A method according to claim 1, wherein the isolated shoot apex is pre-
cultured
prior to division.

7. A method according to claim 1, wherein the method further comprises
culturing
the incised shoot apex.

8. A method according to claim 1, wherein the method further comprises
regenerating a plant from the incised shoot apex.

7




9. A method of transforming canola wherein the method comprises:

(a) isolating a plurality of shoot apices from a plurality of canola
seedlings;
(b) selecting a shoot apex from the plurality of shoot apices;

(c) dividing the shoot apex into at least two pieces thereby exposing a
portion
of the apical meristem therein;

(d) making an incision into a meristematic region of theexposed apical
meristem thereby exposing a portion of the L2 layer therein;

(e) exposing the incised meristematic region to a transformation vector; and
(f) regenerating a plant from the incised shoot apex.

10. A method according to claim 9, wherein two incision are made into the
meristomatic region.

11. A method according to claim 9, wherein transformation with the
transformation
vector is mediated by Agrobacterium tumefaciens.

12. A method according to claim 9, wherein the isolated shoot apex is pre-
cultured
prior to division.

13. A method according to claim 9, wherein the method further comprises
culturing
the incised shoot apex.

8


A single figure which represents the drawing illustrating the invention.

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Admin Status

Title Date
(86) PCT Filing Date 2010-08-12
(87) PCT Publication Date 2011-02-17
(85) National Entry 2012-01-11
Dead Application 2016-08-12

Payment History

Fee Type Anniversary Year Due Date Amount Paid Paid Date
Filing $400.00 2012-01-11
Registration of Documents $100.00 2012-01-31
Registration of Documents $100.00 2012-01-31
Maintenance Fee - Application - New Act 2 2012-08-13 $100.00 2012-07-27
Maintenance Fee - Application - New Act 3 2013-08-12 $100.00 2013-07-29
Maintenance Fee - Application - New Act 4 2014-08-12 $100.00 2014-07-29

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Document
Description
Date
(yyyy-mm-dd)
Number of pages Size of Image (KB)
Abstract 2012-01-11 2 66
Claims 2012-01-11 2 52
Drawings 2012-01-11 3 170
Description 2012-01-11 6 264
Representative Drawing 2012-01-11 1 16
Cover Page 2012-03-15 1 37
PCT 2012-01-11 3 92
Prosecution-Amendment 2012-03-09 1 43
Fees 2013-07-29 1 33