Note: Descriptions are shown in the official language in which they were submitted.
108~ 24909
METHOD AND APPARATUS FOR CONDUCTING FERMENTATION
In fermentation processes such as those involving the aerobic fer-
mentation of microorganisms for the production of single cell protein, vessels
sre normally employed. The vessel size is dependent on the production rate
and varies from laboratory experimental size up to multimillion liter sizes.
Typically, the entire fermentation process is carried out in the vessel, the
process including aerobic fermentation, feeding of nutrient and carbon source,
injection of oxygen, flow of fermentation medium and separation of gas and
liquid phases which involves the use of structure which is generally installed
within the vessel. Fermentation processes are exothermic and release large
quantities of heat which must be removed by heat exchangers which are normally
mounted in the interior of the vessel or can be external of the vessel. Be-
cause of the large amount of heat produced, the heat exchangers must have
large surface area to remove the heat at a rate sufficient to maintain proper
growth temperature within the vessel. This complicates the design and con-
struction of fermentation vessels and increases the physical size thereof.
Because of the amount of components that must be within the vessel, cleaning
and sterilizing is difficult and maintenance of the vessel and components
thereof is complicated.
It is therefore a principal object of the present invention to
simplify the construction of the fermentation apparatus. Other objects and
- advantages of the present invention are: to provide a fermentation apparatus
which permits the use of a vessel having a reduced size and simplified con-
struction; to provide such an apparatus which permits the use of large sur-
face area for heat exchange to adequately remove heat produced by the fermen-
tation process; to provide such an apparatus which can have the ma;ority of
the components thereof constructed from commercially available components;
to provide such an apparatus which has improved operating capabilities; to
provide such an apparatus which can be relatively horizontally disposed so
as to reduce hydrostatic head normally found in vessel type fermentation ap-
paratus; and to provide such an apparatus which is simple to construct, easy
to maintain and well adapted for its intended use.
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Other obJects and advantages of the present invention will be-
come apparent from the following detailed description taken in connection
with the accompanying drawings wherein are set forth by way of illustration
and example certain embodiments of this invention.
FIGURE 1 is a diagrammatic view of a fermentation apparatus.
FIGURE 2 is a diagrammatic view of a modified form of fermentation
apparatus.
As required, detailed embodiments of the present invention are dis-
closed herein, however, it is to be understood that the disclosed embodiments
are merely exemplary of the invention which may be embodied in various forms.
Therefore, specific structural and functional details disclosed herein are not
to be interpreted as limiting but merely as a basis for the claims and as a
representative basis for teaching one skilled in the art to variously employ
the present invention in virtually any appropriate detailed structure.
Referring more in detail to the drawings:
The reference number 1 designates, generally, a fermentation appara-
tus which includes a vessel 2, forming a separation zone, having an elongate
tubular member 3, forming a reaction zone, with an inlet 4 and an outlet 5
communicating with the interior of the vessel 2. Pump means 7 are provided
; 20 to help induce flow of fluid fermentation medium 8 through the tubular member
3 from the inlet 4 to the outlet 5. Heat exchange means 10 cooperates with
the tubular member 3 for removing heat therefrom which is produced by the fer-
mentation. Separator means 11, if desired, can be provided to separate a
portion of the fluid medium into a gas phase and a liquid phase. Injection
means 12 is connected to the tubular member and is operable for injecting an
oxygen-containing gas into the fluid medium as same flows through the tubular
member 3.
The vessel 2 can be of any suitable type and, as shown, is comprised
of a shell portion 14 defining a hollow interior 15. Preferably, the shell
is of a rigid material such as stainless steel for sanitation and corrosion
resistance. The inlet 4 is preferably connected to the vessel 2 adjacent the
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108V~
lower disposed end thereof with the tubular member 3 extending laterally from
the vessel 2 with a major portion of the tubular member preferably being hori-
zontally disposed. As shown, the tubular member 3 is comprised of straight
portions 17 connected in series by return bends 18 with the number of straight
portions 17 being dependent on the reaction zone volume required for the fer-
mentation process. The outlet 5 is connected to the vessel 2 and communicates
with the interior thereof at a position preferably above the inlet 4 for a
purpose later described. Although only one tubular member 3 is shown, it is
to be understood that any number of same can be connected to the vessel 2 as
is required by the desired operating capacity of the fermenting apparatus 1.
Preferably, the tubular member 3 is made from stainless steel pipe sections
which are of generally standard construction and dimension.
The pump means 7 can include and, as shown, includes a motor driven
pump 20 which is positioned adjacent the inlet 4 for pumping fluid medium
from the vessel 2 into the tubular member 3 for circulation therethrough.
Any suitable pump can be employed and is preferably a non-cavitating type.
The pump means 7 can also include, if desired, pumps 21 which cooperate with
the tubular member 3 and have portions thereof suitably mounted in the tubu- -
lar member 3. The pumps 21 can be a turbine type or a serrated propeller
type preferably which are capable of high shear pumping action for the purpose
of mixing gas and liquid phases into foam. The pumps 21 are preferably posi-
tioned between the inlet 4 and outlet 5 of the tubular member to supplement
the pumping or can be used in place of the pump 20 and to help maintain the
fluid medium in a uniformly mixed condition. If a turbine type pump 21 is
used, it can be mounted adjacent a return bend 18 wherein a nozzle or re-
duced diametral portion 22 can be positioned upstream of the pump 21 to
direct the fluid medium flowing through the tubular member into the eye of
the turbine for improved pumping.
The heat exchange means 10 cooperate with the tubular member 3 to re-
move heat generated by the fermentation process taking place in the tubular
member 3. Any suitable heat exchange means can be provided and as shown same
includes a jacket 24 which surrounds the straight pipe portions 17 forming an
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annular flow path therebetween for coolant to flow through. The heat ex-
changer around one straight pipe portion 17 can be connected in series or
parallel with the remainder of the heat exchangers as by conduits between
inlets 25 and outlets 26. Further, baffles (not shown) can be provided in
the annular space between the jacket and the pipe portions 17 to define a
suitable flow path for coolant flowing through the respective heat exchanger.
The injection means 12 can be of any suitable type which is operable
to inject oxygen in some form into the liquid medium flowing through the tubu-
lar member 3. It is to be understood that the terms "oxygen" or "oxygen-
containing gas" can include any suitable form of oxygen, either alone, or in
combination with other substances such as air or oxygen-enriched air. As
shown, the injection means includes a compressor 28 which is connected to the
tubular member 3 by a conduit 29 which extends into the interior of the tubu-
lar member 3. As shown, a sparging device such as a porous member 30 is suit-
ably mounted in the tubular member 3 and is connected to the conduit 29 for
the dispersion of oxygen into the fluid medium. Also, a second porous member
31 can be connected to the conduit 29 forming a porous wall opening into the
- tubular member 3 to allow additional injection of oxygen into the fluid
medium. Preferably, the oxygen is injected in the form of air or oxygen-
enriched air and is taken from the atmosphere and filtered and sterilized as
required through a purification apparatus 32 which is connected to the com-
pressor 28 by a conduit 33. It is to be noted that depending upon oxygen
demand and the length of the tubular member 3, oxygen may be injected into
the tubular member 3 at a plurality of positions along the length thereof be-
tween the inlet 4 and outlet 5 as by having a conduit 35 connecting a porous
member 36 similar to the porous member 30 to the compressor 28. Preferably,
the oxygen is dispersed in small bubbles so as to have a large surface area
contact with a liquid phase by the sparging and also by the action of the
pumps 21. The oxygen-to-liquid ratio is maintained at a value to maintain a
foam condition and adequate for microbial growth.
Oxygen for enriching the air can be injected into the conduit 29
through a conduit 38 by any suitable means (not shown). Also, in the fermen-
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taCion of microorganisms Eor the production of single cell protein certain
nutrients are required which can also be inJected into the conduit 29 through
a conduit 39 by any suitable means (not shown). Such nutrients can include
compounds such as ammonia and related compounds.
In the form of fermenting apparatus shown, the vessel 2 can be pro-
vided with one or more tray members 41 which preferably are secured to the
shell 14 and positioned in the interior of the vessel 2 with same preferably
being positioned at different heights therein. The trays 41 can be of any
suitable structure preferably having an upwardly facing substantially flat
surface 40. Means are provided to define openings or chimneys through the
trays 41 to provide a passageway f~r the upward flow of foam and gas as later
described. As shown, these means include duct members 42 secured to the re-
spective trays 41 and define openings 42' therethrough with the members 42
preventing flow of heavier fluid medium downwardly through the openings formed
thereby. As shown, downcomers 43 are formed for each of the trays 41 to pro-
vide a passageway through which the more dense fluid medium flows downwardly
when same exceeds the level of a dam 44 which defines one edge of the respec-
tive downcomer 43. As shown, the trays are positioned such that the downcomer
of one tray is positioned above the side of the next lower tray, remote from
its respective downcomer whereby the fluid medium flowing over the trays flows
from one edge across the tray to its respective downcomer.
Preferably, the fermenting apparatus 1 is provided with separator
means 11 which is operable to separate foam produced by the fermentation
process into a liquid phase and a gas phase. However, it is to be understood
that chemical defoaming can be used instead of or in conjunction with the il-
lustrated mechanical foam separator. As shown, a conduit 45 is connected to
the upper portion of the vessel 2 and forms a flow path to a second vessel 46.
A mechanical foam breaking device 47, of any suitable type, is provided in the
vessel 46 which breaks the foam into a gas phase, which escapes through an
exhaust conduit 48 or any other suitable gas vent, and a liquid phase which
- is collected in the lower portion of the vessel 46. It is to be noted that
~ a pressure control apparatus 49 can be provided to sense the pressure in the
i
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1080~47
vessel 2 and manipulates a valve 50 located in the exhaust conduit to maln-
tain a desired pressure within the vessel 2 so that the fermentation apparatus
can be operated at a desired controlled pressure at or above atmospheric. Any
suitable pressure controller and valve can be employed. A liquid discharge
conduit 51 is connected to the vessel 46 adjacent the lower disposed portion
thereof and is adapted for the discharge of product-containing liquid from
the vessel 46. Preferably, a suitable level controller 52 is operably con-
nected to the vessel 2 to sense the level of liquid medium therein so as to
manipulate valve 53 for operation thereof in response to level changes to con-
trol the discharge of liquid from the vessel 46 thereby controlling the level
of liquid in the vessel 2. This can also be accomplished by having a conduit
55 form a flow path between the vessel 46 and vessel 2, preferably with a
siphon trap (not shown) therein so that excess separated liquid in the vessel
46 is returned to the vessel 2 to maintain constant inventory therein.
The present invention is more fully understood by a description of
the operation thereof. A nutrient-substrate solution, preferably an aqueous
solution, is introduced into the vessel 2 through an inlet 56 with the nutrient
typically including a carbonaceous material such as methanol and other sub-
stances necessary for the growth of a microorganism within the vessel 2. A
suitable microorganism inoculum is introduced into the vessel 2 for growth
therein. Although it is difficult to define the difference between foam, broth
and liquid in a process of this type, the same vary by the amount of gas dis-
persed in a liquid phase with foam being the highest gas content, broth being
the next higher gas content and liquid being the lower gas content. Foam,
broth and liquid were referred to above as fluid medium. Liquid settles in
the bottom of the vessel 2 and is continually removed therefrom and is pumped
. into the reaction zone or tubular member 3 by the pump means 7. The majority
of fermentation and microbial growth are carried out in the reaction zone.
Oxygen, preferably in the form of air or oxygen-enriched air, is introduced
into the liquid by the injection means 12 to provide the oxygen necessary for
the growth of the microorganism. The heat exchange means extend downstream of
108()~ ~ ~
the points of oxygen introduction for removal of heat as same is produced by
the microbial growth which is enhanced by the oxygen. As described above, a
source of nitrogen as a nutrient can also be injected by the injection means 12
and in a preferred embodiment the source of nitrogen is a base such as ammonia
which can also be used to control the pH of the fermentation process liquid.
The measured pH value is controlled by a suitable pH controller 57 which is
operably connected to the ammonia feed valve 58 which controls the rate at
which ammonia is injected into the broth in the tubular medium. Most of the
fermentation is effected within the tubular member 3 between the inlet 4 and
the outlet 5 as the broth flows therethrough. Fermentation processes are
generally exothermic wherein the heat released during the fermentation process
must be removed by the heat exchanger 10 to maintain the process at a tempera-
ture which is suitable to induce good growth characteristics of the micro-
organism. As described above, oxygen can be injected at a plurality of points
along the length of the tubular member to insure high oxygen concentration in
the fluid medium or ferment as same flows through the tubular member. This is
necessary because the microorganisms must have oxygen to grow and can deplete
the dissolved oxygen in the fluid medium at high rates.
It is to be noted that a uniform broth mixture is desirable and
therefore suitable static mixing devices 59 such as mixing orifice assemblies
can be positioned in the tubular member 3 to help produce a uniform mixture.
; The pumps 21 also help induce flow of broth through the tubular member and
effect some mixing thereof. The fermentation reaction takes place principally
- within the tubular member after which the broth is discharged back into the
vessel 2 at the outlet 5. As described above, a plurality of trays 41 can
be provided in the vessel 2 for increasing the surface area of the broth to
improve the release of dispersed gas from the broth which helps to form the
foam which collects in the upper portion of the vessel 2 and is discharged
; through the conduit 45 into the separator means 11. By use of a horizontally
disposed reactor, i.e., the tubular member 3, the hydrostatic head can be re-
duced in the vessel 2 and thereby decrease the density gradient of the broth
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therein. The foam is a preferred product from the vessel 2 as same normally
contains a higher concentration of protein cells which is a desired end prod-
uct of the fermentation process. It is to be noted that product discharge
can be provided such as at the lower portion of the vessel 2 or any other suit-
able position for removal of product other than at the upper portion of the
vessel or in addition thereto. The foam is separated in the separator means
11 into a gas phase and a liquid phase with the gas phase escaping through the
exhaust conduit 48 and the liquid collecting in the bottom of the vessel 46.
The liquid phase can be discharged through the discharge 51 to other equip-
ment (not shown) for further processing to separate the cell product from the
liquid.
As illustrated, the conduit 55 can be connected between the vessel
46 and the vessel 2 wherein a certain amount of the liquid collected in the
bottom portion of the vessel 46 can be returned to the vessel 2 for further
fermentation. A controller 60 can be operably connected to a remote control
valve 61 which is connected to the conduit 55 to control the rate of liquid
discharge through the conduit 5 into the vessel 2. The controller senses
liquid level in the vessel 46 for control of the liquid level in response to
changes thereof.
The trays 41 can be of any suitable construction having openings 42'
therethrough defined by the members 42 which in effect form chimneys for the
escape of gas and/or foam upwardly. The broth flows over the trays 41 to a
respective downcomer 43 for discharge onto the next lower tray 41 and so on
down the remainder of the trays 41 in the vessel 2 to increase the surface
area of foam for escape of gas therefrom and/or increase the dwell time of
the fluid mediu~ within the vessel 2 to allow the escape of dispersed gases
from the fluid medium. Finally, the broth is discharged from the trays 41
and collects in the lower portion of the vessel 2 as a liquid which is then
recirculated through the tubular member 3 for further fermentation.
It is to be noted that automatic control means in addition to those
shown such as computer control equipment can be provided for the fermentation
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108~ 7
apparatus to monitor various parameters and control the process conductcdwithin the apparatus.
FIGURE 2 SIIOWS a modified form of the present invention wherein
like numbers designate like or similar parts or structure. The reference
numeral 63 designates the modified fermentation apparatus wherein the major
difference between the apparatus 63 is in the configuration of the vessel 2
and the vessel 46. As shown in the modified form, the fermentation apparatus
63 has a single vessel 64 with a overflow weir 65 separating two vessel
portions 66 and 67 with the vessel portion 66 being the equivalent to the
vessel 2 described above and the vessel portion 67 being the equivalent to
the vessel 46 described above. Trays 41 are mounted in the vessel portion 66
for the production of foam and separation of gas from the broth. A foam
breaking device 47 is mounted in the vessel portion 67 for separating the
foam passing over the overflow weir 65 into a gas phase and a liquid phase.
Operation of the fermentation apparatus 63 is similar to operation
of the fermentation apparatus 1.
The following is an example of a typical aqueous fermentation process
carried out in the apparatus as described above with same being calculated on
the basis of the use of bacteria in the aerobic fermentation process using
methanol as the carbon and energy source. Typical operating parameters are
as follows:
Cell yield - 0.39 lb/lb methanol feed;
Oxygen required - 3.0 lb 02/lb cells produced;
Cell concentration - 3.0 weight percent in fermenter broth,
6.0 weight percent in liquid from foam separator;
Reaction residence time - 3 hours;
Heat of fermentation - 18,000 BTU/lb of cells;
Total flow velocity of medium in tubular member - 16 ftlsec;
Liquid/gas ratio in tubular member - approximately 1 vol/vol;
Tubular member input pressure - 45 psia;
Foam separator pressure - approximately 42 psia;
Fermentation temperature - 40C (104F).
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For a fermenting apparatus havLng an approximately 10,000 gal.
capacity, the following conditions would be typical as based upon the above
parameters:
Cell production rate - approximately 417 lb/hr;
Tubular member - 16-40 ft. sections of 18 in. pipe with return
bends connecting the straight sections;
Heat removal - jacket each section with 20 in. pipe and supply
cooling water at approximately 86F in and 91F out with
total cooling water being supplied at 3,000 GPM. Four
sections of heat exchangers are connected in series
(thereby producing four parallel sets) to obtain the ap-
proximate 6 ft/sec cooling water velocity in the annulus
or provide baffles in the annulus to lengthen flow path of
cooling water and connect all sections in parallel.
Gas dispersion - disperse approximately 14.1 cfs of air input
(at 45 psia 104F or 2350 cfm at 14.7 psia and 60F)
in the tubular member;
Foam separator - (vessel 2). The vessel would be approxi-
mately a 7 ft. diameter by 10 ft. in height vessel
having three trays with approximately seven l-ft. di-
ameter openings therethrough and having an overflow weir
preferably less than or equal to approximately 1 ft.
in height;
Gas separator - (vessel 46). The vessel would be approxi-
mately 4 ft. in diameter by 7 ft. in height;
Fluid medium circulation - approximately 6350 GPM;
Pressure drop through each length of tubular member -
approximately 1 psi, based on the use of a pump 21
positioned at the end of every other pipe section,
i.e.5 the use of seven pumps 21.
Cooling water - flow rate 3000 GPM - 5F rise from coolant
inlet to outlet;
Power required - pumps or impellers approximately 150 hp total
foam breaker 40 hp
air compressor 230 hp
420 hp
The above values would be for a typical fermentation apparatus as
disclosed but it is to be understood that the values will vary depending upon
- the type of microorganism used, fluid medium used and the like.
It is to be understood that while I have illustrated and described
certain forms of my invention, it is not to be limited to the specific form
or arrangement of parts herein described and shown.
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