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TITLE OF THE INVENTION
SAFFLOWER PRODUCTS AND'METHODS FOR THEIR PRODUCTION
BACKGROUND OF THE INVF'.NTrnN
The present invention relates to a safflower seed, a
safflower plant, a safflower oil, a safflower seed product, a
safflower variety and a method for producing a safflower
seed.
Safflower, Carthamus tinctorius L., is a highly
branched, herbaceous, thistle-like annual varying in height
from 30 to 150 cm. Safflower has been grown for centuries in
small plots over a vast area from China to the Mediterranean
region, and along the Nile Valley as far as Ethiopia. Since
1945 commercial production has taken place in the United
States, Russia, Australia and Mexico. Safflower has remained
a minor oilseed in terms of total production and world trade.
The crop can be grown under full mechanization sa that the
production costs compare favorably with other oilseeds. In
addition, safflower is suitable for growth in drier regions
and thus provides a profitable alternative in such areas.
Altering the oil content, increasing protein content or in-
creasing seed yield are current objectives in safflower
breeding programs.
Safflower oil is comprised primarily of palmitic,
stearic, oleic and linoleic acids, with the vast majority
comprised of oleic and linoleic acids. Two different types
of oil can be produced, depending on the genetics of the par-
ticular safflower line. One type is high in linoleic acid
and the other is high in oleic acid. A third experimental
type of safflower oil contains about equal amounts of oleic
° and linoleic acids. A high oleic acid concentration of
safflower oil is desirable when its end use is in the food
industry. A high linoleic acid safflower oil is desirable
when its end use is in resin and paint formulations. In all
instances, it is desirable to produce a safflower ail which
is low in the saturated fatty acids, i.e., palmitic and
stearic acids.
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Three allelic genes, Ol, ol' and ol, are the primary
genetic determinants of the proportions of oleic and linoleic
acids. The genotype olol has 72-80% oleic acid in the oil, .
and the genotype O O has 72-80% linoleic acid. The genotype
has about 45% of each fatty acid. It has been discov- ,
Bred that additional minor genes may be involved in determin-
ing the fatty acid content of a particular safflower variety.
Knowles, P.F., J. Am. Oil Chem. Soc. 49, 27 (1972) noted that
such modifying genes may make it possible to raise the oleic
acid content above 80%, but probably not above 85%.
Knowles, supra, further noted that although high oleic
and high linoleic safflower varieties are temperature stable,
there is a slight variation in fatty acid content depending
on the growth temperature of the variety. This variation is
illustrated in Table 1.
TABLE 1
Fatty Acid Composition of Safflower
Types Grown Under Different Temgeratures
l 2 Fatty Acid (%j
Oil TypeTemp. Sat. Oleic Linoleic
high Low I 8.4 9.1 82.4
linoleicInt. 7.8 14.0 78.2
High 9.3 15.2 75.5
high Low 6.3 69.7 24.0
oleic Int. 7.0 71.6 21.4
High 8.3 77.4 14.3
1 Day and night temperatures, respectively: low = 18.4'C
and 15.6'C; intermediate - 26.7'C and 23.9'C; and high -
29.4'C and 26.1'C.
2 Saturates = palmitic and stearic acids.
Thus, a high oleic acid safflower variety grown in Montana,
for example, having a mean growing temperature of about 70'F,
would be expected to have a slightly lower production of
oleic acid than the same variety grown in California.
Conversely, the variety grown in California would be expected
to have a higher level of oleic acid than the value reported
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for the variety in Montana.
~'SUI~~iARY OF THE INVENTION
The present invention relates to a safflower seed, a
safflower plant, a safflower oil, a safflower seed product, a
safflower variety and a method for producing a safflower
seed.
More specifically, the invention relates to a safflower
seed having an oleic acid content of about 80% or greater
relative to the total fatty acid content of the seed when the
seed is produced at a mean temperature of about 70'F. The
invention further relates to a safflower seed having a
linoleic acid content of about 80$ or greater relative to the
total fatty acid content of the seed when the seed is
produced at a mean temperature of about 70'F.
The invention also relates to a safflower plant which
produces seeds having either the above described content of
oleic acid or linoleic acid when the plants are grown in an
environment in which the mean temperature during seed
development is about 70'F.
The present invention further relates to a method of
producing the disclosed safflower plants and seeds by
crossing a female parent and a male parent. Each parent has
been developed by a series of crosses of several safflower
varieties. The progeny of the cross of the female and male
parents is further selected for either high oleic acid or
high linoleic acid content and selfed to produce a further
generation having an enhanced oleic acid or linoleic acid
content. The selection arid selfing steps may be repeated
many times.
The invention also relates to a safflower oil having an
oleic acid content of about 80% or greater relative to total
fatty acid content which has been derived from seed produced
at a mean temperature of about 70'F. Finally, the invention
relates to a safflower oil having a linoleic acid content of
about 80% or greater relative to total fatty acid content
which has been derived from seed produced at a mean
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temperature of about 70'F.
A safflower seed having an oleic acid content of
approximately 80% or greater, relative to the total fatty
acid content of said seed, and a ratio of the amount of
saturated fatty acid in said seed to the amount of oleic acid
in said seed of less than about 0.08.
A safflower seed having an oleic acid content of about
80% to about 90% relative to the total fatty acid content of
said seed and wherein the ratio of saturated fatty acids to
the oleic acid is less than about 0.08.
A safflower plant having an oleic acid content of
approximately 80% or greater, relative to the total fatty
acid content of said seed, and a ratio of the amount of
saturated fatty acid in said seed to the amount of oleic acid
in said seed of less than about 0.08.
A safflower plant having an oleic acid content of about
80% to about 90% relative to the total fatty acid content of
said seed and wherein the ratio of saturated fatty acids to
the oleic acid is less than about 0.08.
A saffhwer seed having an oleic acid content of
approximately 80% or greater, relative to the total fatty
acid content of said seed, wherein said seed is a product of
a cross between (A) a first parent from a safflower line
which is true-breeding for said oleic acid content and (B) a
parent from a second safflower line.
An individual safflower plant wherein the seeds there-
from have an oleic acid. content of approximately 80% or
greater, relative to the total fatty acid content of said
seeds, wherein said plant is a product of a cross between (A)
a first parent from a safflower line which is true-breeding
for said oleic acid content and (B) a parent from a second
safflower line.
A safflower ail produced by the process comprising '
extracting oil from a substantially homogeneous assemblage of
safflower seeds, said safflower oil having an AOM value of
greater than about 60 hours.
A safflower oil having an oleic acid content of about
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80% or.greater, relative to the total fatty acid content of
the oil wherein the oil is substantially free of free fatty
acids.
A bleached safflower oil having an oleic acid content of
about 80% or~ greater, relative to the total fatty acid
content of the oil.
A deodorized safflower oil having an oleic acid content
of 80% or greater, relative to the total fatty acid content
of said oil.
A safflower oil having an oleic acid content of 80% or
greater, relative to the total fatty acid content of said
oil, wherein the oil is deodorized, bleached and is substan-
tially free of free fatty acids.
A safflower seed having a linoleic acid content of about
80% or greater, relative to the total fatty acid content of
said seed when said seed is produced at a mean temperature of
about 70'F.
A safflower plant which at a mean seed development
temperature of about 70'F produces seed having a linoleic
acid content of about 80% or greater, relative to the total
fatty acid content of said seed.
A Carthamus tinctorius L. seed product consisting of a
substantially homogeneous assemblage of safflower seeds
having a linoleic acid content of about 80% or greater,
relative to the total fatty acid content of said seeds when
said seeds are produced at a mean temperature of about 70'F.
A safflower variety consisting of a substantially
uniform population of Carthamus tinctorius L. plants which at
a mean seed development temperature of about 70'F produces
seeds having a linoleic acid content of about 80% or greater,
relative to the total fatty acid content of said seed.
A method for producing a safflower seed having a
linoleic acid content of about 80% or greater, relative to
the total fatty acid content of said seeds when said seeds
are produced at a mean temperature of about 70'F, comprising
the steps of (a) making a series of crosses of safflower
varieties to produce a female parent, (b) making a series of
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crosses of safflower varieties to produce a male parent, and
(c) crossing said female parent with said male parent.
A safflower oil having a linoleic acid content of about
80% or greater, relative to the total fatty acid content of
said oil, said oil obtained from safflower seed produced at a
mean temperature of about 70'F.
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DETAILED DESCRIPTION OF THE INVENTION
In order to provide an understanding of several of the
terms used in the specification and claims, the following
definitions are provided.
Homogeneous assemblage - The terns homogeneous assemblage
is intended to refer a group of seeds which are homogeneous
for a given characteristic. This terra is intended to include
any seeds which are homogeneous as determined by any 1000
seeds from one acre of safflower.
Mean temperature - The mean temperature is the mean of
the recorded daily temperatures during a specified period,
such as a-month or a developmental stage of safflower growth.
The mean temperature (F) for the months of May through
October from 1949 through the 1988-89 crop year is shown in
Table 2.
TABLE 2
Mean Temperatures ('F) for 1949-89 at the Eastern
Agricultural Research Center, Sidney Montana
onth Mean Temp. Mont Mean Temp.
May 55.5 Aug. 67.8
June 64.2 Sept. 56.4
July 69.7 Oct. 45.6
Saturated Fatty acids - As used herein, the term
saturated fatty acids is intended to include only palmitic
acid and stearic acid.
Seed development or production - For the purposes of
this invention, the seed development, seed production or
. production of seed is the time during safflower growth from
pollination through seed maturation. In Montana, this time
is usually from about the third week of July through the
middle of August.
Series of crosses - A series of crosses is used herein
to refer to the breeding technique of transgressive segrega-
tion in which multiple, single crosses are made to praduce
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female and male parents.
As noted above, the content of various fatty acids such
as oleic and linoleic, which is characteristic of oil from a
given safflower seed sample, is commonly expressed as a
percentage of the total fatty acid fraction in the oil. This
convention will be followed in the description given below
unless otherwise indicated. Dimensionless ratios of saturat-
ed fatty acid content to either oleic or linoleic acid
content, which are also mentioned below, are calculated by
dividing the saturated fatty acid percentage by either the
oleic or the linoleic acid percentage, as applicable. As
also noted above, the specific level of oleic acid, linoleic
acid and saturated fatty acids is dependent upon the tempera-
ture during seed development. Unless indicated otherwise,
the percentages shown for the contents of these fatty acids
are for safflower grown in Montana and at a mean temperature
of about 70'F for seed development or production. As used
herein, about 70'F is intended to include mean temperatures
of 70'F, plus or minus 2'F.
The present invention has a low level of saturated fatty
acid in the oil. The fatty acid content in a ratio to the
oleic acid is expressed as the combined weight of the two
saturated fatty acids divided by the weight of the oleic
acid. The measurement of the ratio is done using the
extracted fatty acids, however, the oils may also be used
because there is little difference in the methods and little
difference in the weight of the oil versus the fatty acids.
The ratios for the saturated fatty acids to the oleic
acid are thus preferably less than about 0.08, more
preferably less than about 0.07, yet more preferably less
than about 0.06. '
For purposes of the present description, the terms
"variety", "cultivar°' and "line" are used synonymously to
refer to a group of plants which are uniform in their traits
such that there is relatively minor variation within the
group and such variation can be characterized. The decreased
variation within this group has generally (although not
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exclusively) resulted from several generations of self-polli-
nation or selfing. A variety is considered true breeding for
,, a particular trait if it is genetically homozygous for that
trait to the extent that when the variety is self-pollinated,
a significant amount of independent segregation of the trait
among progeny is not observed.
Safflower is primarily self-pollinating, with some
cross-pollination possible through the interaction of
insects, primarily bees. Safflower pollen is too heavy for
wind-aided cross-pollination to occur. Consequently, in arid
areas, such as in Montana, there is little cross-pollination
that occurs because of a low bee population. Safflower
breeding, as described herein, is performed using convention-
al techniques to develop the high oleic acid and high
linoleic acid varieties described and claimed.
The safflower of the present invention reproducibly'
expresses a high oleic acid trait or a high linoleic acid
trait, and conversely, a low saturated fatty acid trait,
against a phenotypic background of agronomical traits which
is sufficiently consistent for commercial applications. In
contrast, the original breeding material produced seeds
having either a lower oleic acid content or a lower linoleic
acid content, and conversely, a higher saturated fatty acid
content against a similar phenotypic background of agronomic
traits. In accordance with one embodiment of the present
invention, safflower seed is produced which contains in
excess of 80%, preferably in excess of 81.9%, and most
preferably in excess of 83.4%, up to 90%, oleic acid when
produced at a mean seed development temperature of about
70°F. In a second embodiment of the present invention,
safflower seed is produced which contains in excess of 80%,
preferably in excess of 83.0%, and most preferably in excess
of 86.0%, up to 91%, linoleic acid when produced at a mean
seed development temperature of about 70°F.
To produce. the safflower varieties of the present
invention, an extensive breeding program is used in which
many parental varieties are utilized to pursue transgressive
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segregation. In order to accumulate all minor genes for
various traits, it is necessary to keep the selection
pressure low. In this breeding program varieties having the
maj or ~,, gene ( for high oleic) or the maj or 03, gene ( for high
linoleic) are utilized and many crosses are made to accumu-
late minor, favorable genes into one genotype. Any genotype
having desirable characteristics may be utilized in this
breeding program. The intermediate lines which are produced
are not crossed with any of the original breeding material.
The genotypes are then selfed and selected for the specific
level of oleic acid or linoleic acid desired.
This general breeding procedure has given attention to
the modifications in fatty acid composition and other
desirable traits produced by genes having small effects on
such traits. Since 1973, cultivars have been crossed and
intercrossed on an annual basis to permit selection for
transgressive segregation of superior lines for the desired
traits, including oil quality, Alternaria leaf spot resis-
tance, Pseudomonas bacterial blight resistance, oil content,
drought resistance and other quantitative traits. Each year,
selections have been made for traits or characteristics that
are expressed. -Approximately the top 50% of the breeding
material is saved from each cycle of selection for inter-
crossing for the next cycle of selection arid intercrossing.
In this manner genes with small effects for desirable traits
such as fatty acid composition, disease resistance, drought
tolerance, etc., are incorporated into the continually
improving germplasm.
Newly developed cultivars with the additional favorable
genes are incorporated into the germplasm base by intercross-
ing with superior breeding lines. During the period from '
1973 to 1982, selection was practiced to improve seed oil
content, Alternaria leaf spot resistance, Pseudomonas
bacterial blight resistance, Sclerotina head rot resistance,
posthoused seed dormancy, drought resistance and other
expressed and desirable traits. For example, seed oil
content of the breeding material was improved approximately
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1% each year, from 33% to 42% during the period from 1974 to
1982. Selection for improved fatty acid composition, either
oleic acid content or linoleic acid content, began in 1985.
Selection for transgressive segregation of superior lines for
fatty acid composition has been practiced since 1985. oil
types have been obtained which are lower in saturated fatty
acids with, corresponding higher levels of oleic acid or
linoleic acid than previously reported.
The top 302 lines for oleic acid productions were
selected from a group of 6000 crosses which were made in
1985. The 1985 lines produced from about 70.9% to 80.8%
oleic acid in the seed. The linoleic acid content ranged
from 12.4% to 22.1% and the saturated fatty acid content
ranged from 4.7% to 6.8%. The ratio of saturated fatty acids
to oleic acid ranged from 0.059 to 0.088, with two lines at
0.093 and 0.095. Only one of the 302 lines was above 80%.
These 302 lines were selfed in 1986 and the seed
analyzed for oleic acid production. A total of 215 lines
were selected after the 1986 season which produced seeds
having an oleic acid content greater than 80%. The range in
oleic acid content of these lines was 80% to 84.7%. The
linoleic acid content ranged from 8.4% to 13.6% and the
saturated fatty acid content ranged from 3.9% to 7.5%. The
ratio of saturated fatty acids to oleic acid ranged from
0.047 to 0.086, with one line at 0.093. Twenty-four of these
lines produced seeds having an oleic acid content of greater
than 83%.
These 215 lines were selfed in 1987 and the seed
analyzed for oleic acid production. A total of 303 lines
were selected after the 1987 season which produced seeds
having oleic acid content greater than 80%. The range in
oleic acid content of these lines was 81.9% to 85.9%. The
linoleic acid content ranged from 7.2% to 11.8% and the
saturated fatty acid content ranged from 3.7% to 6.2%. The
ratio of saturated fatty acids to oleic acid ranged from
0.044 to 0.076. forty-eight of these lines produced seeds
having an oleic acid content of greater than 84%.
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These 303 lines were selfed in 1988 and the seed
analyzed for oleic acid production. A total of 352 lines
were selected after the 1988 season which produced seeds
having oleic acid content greater than 80%. The range in
oleic acid content of these lines was 83.4% to 86.1%. The
linoleic acid content ranged from 7.4% to 10.4% and the
saturated fatty acid content ranged from 4.7% to 5.8%, with
six lines up to 6.5%: The ratio of saturated fatty acids to
oleic acid ranged from 0.055 to 0.070, with three lines up to
0.075. Twenty-two of these lines produced seeds having an.
oleic acid content of greater than 85%.
These 352 lines were selfed in 1989 and the seed
analyzed for oleic acid production. Slight changes were
seen in the oleic acid content of the progeny, with some
lines approaching 87%. In order to increase the oleic acid
content to greater than 87%, and up to 90%, the previously
obtained lines are intercrossed to derive new lines. These
new lines produce seed having an oleic acid content of up to
90%.
The safflawer varieties produced in accordance with the
present invention share characteristics common among commer-
cial safflower varieties. Several of these characteristics
will be set forth in more detail below.
EXAMPLES
The following examples are provided to further illus-
trate the present invention and are not intended to limit the
invention beyond the limitations set forth in the appended
claims.
EXAMPLE 1
Determination of Fatty Acid Content
The fatty acid composition of safflower seeds developed
in the breedyng program was determined by gas-liquid
chromatography (GLC) in accordance with the procedures
described in Hougen, F.W. and Bodo, V., J. Am. Oil Chem. Soc.
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50, 230 (1973) and Daun, J.K. and Mazur, P.B., J. Am. Oil
Chem. Soc. 60, 1751 (1983); and, with techniques developed by
D.T. McGregor, Agriculture Canada Research Station,
Saskatoon, Saskatchewan, Canada for rapeseed and mustard.
A) Analysis of 12 Gram Samples
A 12 gram sample of the safflower seed of interest was
dried at 45-50'C for at least 4 hours. The sample was then
ground in the presence of 40 ml of hexane using a tissue
homogenizer for approximately 45-60 seconds. The container
of the homogenate was then capped and allowed to settle for
approximately 15 minutes. A 0.5 ml aliquot was then pipetted
in a 13x100 mm glass test tube. To this tube Was added 0.5
ml of benzene followed by 2 ml of sodium methoxide in
methanol at a concentration of 2 g sodium methoxide in 100 ml
methanol. The tube was capped and allowed to stand for 10
minutes. After standing, 3 drops of Bromophenol Blue in
ethanol Was added followed by drops of 1N HC1 until the blue
solution turned yellow. The excess HC1 was then neutralized
with 1N sodium carbanate until one drop turned the solution
blue. Deionized water was then added to make sure separation
of the two phases was complete. Approximately~l ml of the
upper phase was then placed in a septum sealed vial and
analyzed by GhC.
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B) Analysis of 1 to 5 Seed Samples
The individual seeds were cut in half with a razor
blade. The halves were then placed in a glass test tube with ,
a small amount of hexane, approximately 1-2 ml. The pieces
were then mashed and stirred for around 30 seconds. with a
glass rod and allowed to sit with the tube capped for 15
minutes. A 0.5 ml sample of the hexane extractant was then
treated in the manner described above. The fatty acid
analysis of half a seed enabled the planting of the remaining
half for further breeding.
C) Gas-Liquid Chromatography
The GLC analyses were accomplished using a 5890
Hewlett-Packard in.~~«rument equipped with a flame ionization
detector and a Hewlett-Packard 3392 integrator. The column
used was a J&W Scientific fused silica capillary column
coated with DB-225+ (film thickness of 0.25 microns, column
dimensions of 15 meters x 0.318 mm, catalog no. 1232212. The
operating conditions of the GLC included an injector tempera-
ture of 225°C and a detector temperature of 300°C. Column
flow was 1.6 cc/min. of helium. Each chromatographic run was
temperature programmed to begin at 170°C and remain at that
temperature for 2.0 minutes. The temperature was then
increased to 190°C at a rate of 3°C/min. The temperature was
then held at 190°C for 1.5 minutes. The column temperature
was increased to 220°C at a rate of 2°C/min. The temperature
was held at 220°C for 3 minutes. After this period of time,
the chromatograph was completed and the column prepared for
the next run.
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Example 2
Characterization of Parent Lines
The following is a description of several of the parent
safflower lines utilized in the breeding program. Any parent
material could be utilized as long as it met the criteria
described above, namely the presence of desirable traits such
as oil content, blight resistance and the like. These parent
materials are readily available from public institutions or
for sale by commercial organizations.
'Frio' safflower developed by the Arizona Agricultural
Experiment Station (AES) and the Agricultural Research
Service - U.S. Department of Agriculture (ARS-USDA) was
released in 1965 and has the genotype O O1 that produces seed
high in linoleic acid.
'Sidney Selection 87-14-6'is a 1965 selection for
AlteZrnaria leaf spot resistance made from the 1964 bulk
composite of 555 safflower introductions of the 1960 world
safflower collection.
'79AZ9543-1' is an experimental white flowered oleic
safflower line obtained in 1980 from the Arizona AES.
'S-304' was developed by Seedtec International, Woodland
California and has the genotype o~'q~' that produces an oil
with approximately equal amount of linoleic and oleic acids,-
but the relative amounts of each fatty acid are greatly
influenced by growing temperature.
'S-208' was developed by Seedtec International and is a
normal white hull seed variety and has the genotype Ol0 that
produces seed high in linoleic acid. A white flowered
individual plant from S-208 was used as one of the parental
lines.
'UC-1' was developed in 1966 by the California AES and
was the first,commercial oleic safflower cultivar having the
genotype olol that produces seed higher in oleic acid
content.
'Oleic Leed' is a high oleic acid content cultivar
released in 19?4 by the ARS-USDA and the California AES. The
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oleic acid o of gene was obtained from 'UC-1' crossed to
'Deed' and backcrossed~to 'teed' two times.
'Th-5' is a parental line developed by the ARS-USDA and
the Utah AES in 1970 arid carries the thth gene pair which
governs reduced pericarp. The line was released for use as a
female parent in hybrid development programs.
'Carthamus ' d s' is a related species to C.
tinctorius L. with 12 chromosome pairs.
'0141-1' is an individual plant selection with extremely
reduced hull or partial hull, with an iodine value of 141
obtained from A.L. Urie in 1974. This plant was obtained
from a line segregating for linoleic gene O1 and oleic gene
ol.
'US-10' was developed by the ARS-USDA and the California
AES in 1959 and has the genotype 0101 that produces seed high
in Iinoleic acid. A white flowered individual plant from
'US-10' was used as one of the parental lines.
Examples 3-9 are directed to the development of high
oleic acid varieties.
Example 3
1985 Breeding Program and Results
The top 302 lines for oleic acid production were
selected from a group of 6000 crosses which were made in
1985. The 1985 lines produced from about 70.9% to 80.8%
oleic acid in the seed. The linoleic acid content ranged
from 12.4% to 22.1% and the saturated fatty acid content
ranged from 4.7% to 6.8%. The ratio of saturated fatty acids
to oleic acid ranged from 0.059 to 0.088, with two lines at
0.093 and 0.095. Only one of the 302 lines was above 80%.
The dean temperatures for July and August were 71.4'F and
66.3°F, respectively. The results.are shown in Table 3.
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Example 4
1986 Breeding Program and Results
These 302 lines were selfed in 1986 and the seed
analyzed for oleic acid production. A total of 215 lines ,
were selected after the 1986 season which produced seeds
having an oleic acid content greater than 80%. The range in
oleic acid content of these lines was 80% to 84.7%. The
linoleic acid content ranged from 8.4% to 13.6% and the
saturated fatty acid content ranged from 3.9% to 7.5%. The
ratio of saturated fatty acids to oleic acid ranged from
0.047 to 0.086, with one line at 0.093. Twenty-four of these
lines produced seeds having an oleic acid content of greater
than 83%. The mean temperatures for July and August were
68.9'F and 68.0'F, respectively. The results are shown in
Table 4.
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WO 91/11906 PCT/US91/00917
.~s~~:.~v,
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WO 91/II906 PCT/US91/00917
-38-
., . ~ . 2C~5143~
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WO 91/11906 PGT/US91/00917
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WO 91!11906 PGT/US91/00917
,;-,-.
~:~ ~.=:
20.5.1435 -4 0
., x.
Example 5
1987 Breeding Program and Results
These 215 lines were selfed in 1987 and the seed
analyzed for oleic acid production. A total of 303 lines
were selected after the 1987 season which produced seeds
having oleic acid content greater than 80%. The range in
oleic acid content of these lines was 81.9% to 85.9%. The
linoleic acid content ranged from 7.2% to 11.8% and the .
saturated fatty acid content ranged from 3.7% to 6.2%. The
ratio of saturated fatty acids to oleic acid ranged from
0.044 to 0.076. Forty-eight of these lines produced seeds
having an oleic acid content of greater than 84%. The mean
temperatures for July and August were 71.3'F and 66.0'F,
respectively. The results are shown in Table 5.
WO 91/11906 PGT/US91/00917
-41- w ~~ ~ °' « ~~ c::;
.:=: ~.~'.. ~~~J
'itIBLL S
'::/sMS%
uNmc sr~Rrc :~o~ieuNOmc
16:0 18:0 1831!::"18:2
~ j
7b3074-1 3.5 1.4 85:9:;:7.2
7b2377-2 3.2 1.1 85:3 8.6
.
7b3151-i 3.2 1.2 85:0.::::8.6
7b3144-5 3:3 1.1 84.g 8.6
7b3155-2 3.3 1.1 84:gf 8.8
7b336t-2 3.4 1.4 84:8.:8.5
7b3083-2 3.8 1.7 8a:8:~7.4
7b23771 3.2 1.2 84:8':8.8
7b3203-2 3Ø 0.7 84:7:.10.2
7b3081 2 3.8. . 1.9 847.:.7.4
. .
.,.
..
- 7b3424-2 3.1 0.9 , 9.3
847'
."..,:
7b35231 3.2 0.9 ~ 84~T9.3
'
7b3154- 3.3 1.1 ;' 9.1
1 84T<:
7b2382-2 3.1 1.1 ~ .84:8;9.5
,
7b2381-3 3.4 1.1 84:8: 8.9
7b3075-2 2.9 1.2 84:5::9.4
;
7b2382-1 3.1 1.2 84:5: 9.2
7b30781 3.2 1.2 84.5r 9.0
7b3074-4 3.6 1.7 84:5:;.8.1
7b2376-2 3.1 1.1 84.4 9.4
~
7b3157-1 3.1 1.1 84:4' 9.2
7b3497-2 3.4 1.1 .:84:14- 9.6
7b31442 3.6 1.1 ~ 84;48.8
Tb3151 2 , 3.1 1.0 . 84:49.4
7b349&2 3.2 1.0 ~84:3~9.5
7b532-2 3:3 0.8 843 10.2
7b2382-4 3.2 1.0 84:3 9.3
SUBSTITUTE SHEE7~
WO 91/11906 PCT/US91/00917
. . . ,. .~p5143~ -42-
:; .~ ,
TABLL 5 (Continue)
/s % ....%'.::.;
ENTRY PALMITiC STEARIC LINOLEIC
:OtQC
7b3151-2. 3.1 1.0 84:3::.:9.7
_ _
~~2 3.6 1:3 84:3 8.9
r:
7b3497-3~3.5 1.0 84:2': 9.1
7b3498-23.5 1.1 84:2. 9.3
?b3524-23.2 1.1 84.2:. 9.5
7b3151-43.0 1.0 814.2 9.8
~
7b2381-13.4 0.9 84:1:' ~ 9.4
7b3148-23.2 1.1 84:1 9.7 '
~
7b3168-23.2 1.3 84:1 9.4
7b3764-13.9 1.0 84:1 8.8
7b3156-13.1 1.1 84.1 9.5
7b3159-13.1 1.0 84.1.,..9.9
7b3589-13.6' 1.0 84.1' 9.4
7b3076-23.2 1.8 84Ø; 8.8
7b3082-43.6 1.5 84.0:;. 8.5
7b3589-13.2 1.1 ~ 84:fl,;.11.2
7b3523-13.5 1.1 :84:0.::9.4
7b3211-13.3 ~ 1.3 ~B4:0~.:9.5
~7b3333-23.4 1.5 ~ 84Ø 8.9
:
,
7b3155-13.6 1.0 83.9:% 9.3
7b3325-i3.4 1.6 83.9 8.9
w
7b3498-13.4 1.1 83.9 9.8
:
7b3496-13.3 1.1 .83:9 10.0
7b3361-13.7 1.8 83:9:': B.4
7b3166-13.1 1.0 v 83:9':':10.1
7b2377-13.2 1.3 83:9: 9.4
7b532-7 3.3 1.2 ~ 83.9::9.7
7b2376-53.2 1.0 ~ 83.3 9.9
7b2381-23.4 0.9 83.8. 9.8
7b3179-13.5 0.6 83.8. 10.6
SUBSTITUTE BHEEl
WO 91/11906 PGT/US91/00917
(/.~-~'!~.u
~a-.;;
-43- . , . . . ;,w:1,.
., % iv -'~.y.y~j
TABI~ 5 ( Continued)
F1EL0 % % . ~../s
:'
ENTRY PALMIT1C STEARIC LINOLEIC
~OLE~C
7b2382-2 3.4 1.0 ~ 83:8:'r9.5
7b3449-1 3.4 1.8 83:8:'' 9.0
7b3424-5 3.1 1:1 83:8::: 10.0
7b3764-2 3.4 0.8 83:?' 10.0
~
7b3157-2 3.2 1.1 83:7 9.9
~
7b2382-1 3.2 1.2 ~ 8~:?':9.7
7b2205-2 3.4 1.1 83;T.".'9.8
'
7b3158-2 3.2 1.3 83.7. S.7
'
7b3588-1 3.3 1.2 83:1:: 9.9
7b3201-1 3.3 1.0 83.7 9.9
~
7b33622 3.4 2.0 83.7" 8.7
7b532-5 3.5 0.9 83.7" 9.9
?b3152-2 3.1 1.1 83:7'.' 10:1
7h3523-3 3.7 0.9 83.7 9.7
'
Tb3217-1 3.3 1.4 83.fi 9.5
7b3589-2~3.3 1.2 B3':fi 9.9
7b3151-5 3.3 1.1 83:6., 10.0
7b1111-2 3.1 0.8 83:6~.r 10.7
7b3502-1 3.3 1.0 83:6: 10.0
7b333fi-23.4 1.5 83.fi 9.4
v.
7b2376-4 3.1 1.0 83.fi:: 10.2
7b2381-1 3.2 0.9 83.fi 10.2
7b3217-2 3.5 1.2 83.5 10.0
''~
7b3302-2 3.5 1.5 83:5:~~ 9.3
7b3082-2 3.6 1.9 83:5:: 8.6
7b3501-1 3.4 1.1 83.5:: 10.3
7b3164-1 3.3 1.0 83:5:: 10.1
'
7b3149-1 3 3 1.1 83:5 9.9
7b3261-1 3.5 2.1 83.5.. 8.7
7b2378-1 3.3 0.9 83.5. 10.7
SUBSTITUTE SHEET
WO 91/11906 PGT/US91/00917
~~0~~.4a~
.. ;,... ,. . __., ~...~: _44_
TABLE ~ (Continued)
fo: "~:
ENVY PALMITiC STEARIC UNOLEIC
OLEIC
7b3493-2 3.6 1.0 83 4:: 9.9
7b3502-2 3.4 1.1 83:4:: 10.2
7b3076-7 3.2 1.7 83.4'-. 9.7
7b3912-Z 3.4 1.4 83.4 9.9
'
7b3246-1 4.2 1.0 83.4 3.5
~~
Tb3201-2 3.2 1.0 83:4,~~ 10.2
7b3349-1 3.3 1.5 83.4:: 9.9
7b3588-3 ~ 3.4 1.2 83.4- 9.9
7b3226-1 3.6 2.0 83.3. 9.2
?b494-1 3.9 t 83.3 9.9
.4
7b3166-2 3:2 1.1 83.3 10.3
7b2378-1 3.3 0.9 83.3 10.5
7b3311-1 3.2 1.6 83.3 10.1
~
7b3412-1 3.2 1.2 83.3'. 10.5
.
7b2382-3~ 3.4 t.2 83:3. 9.8
7b3332-2 3.4 1.5 83.3 9.7
7b3236-1 3.4 1.7 83.3. 9.3
7b2378-2 3.2 0.9 83:3. 10.4
7b3326-i 3.4 1.6 83.3 9.8
,
7b3521-3 3.4 1.0 83.3;'' 10.2
7b3238-1 3.7 2.0 83.2. 9.1
7b3449-2 3.5 1.6 83.2: 9.7
~
7b3881-2 3.3 0.7 83.2.. 10.7
7b3409-2 3.7 1.8 83.2.:'.9.4
7b3268-4 4.2 2.8 832., 8.3
7h3766-2 3.3 1.0 83:2.: 10.5
7b3333-1 3.4 1.4 83:2. 9.8
7b3588-5 3.3 1.0 83.2" 10.4
~
7b3544-2 83.2 16.8
7b3588-4 3.4 1.2 83.2 10.2
SUBSTITUTE SHEET
WO 91!11906 PGTlUS91/0~0917
.,.,;~
TABLE 5 (Continued)
FIELD % % %: ' %
ENTRY PAL-MITICSTEARIC'OLEIC LINOLEIC
7b3349-2 3.2 1.7 83:2'' 9.9
7b3408-3 3.6 1.4 83:1 10.1
~
7b2381-5 3.4 1.0 83:1 10.3
'
7b3235-3 4.0 1.5 83:1 9.2
7b3206-2 3.4 t .2 83:1 t 0.
t
7b3341-1 3.6 2.1 83:1 9.1
7b3212-2 3.2 1.1 83.1 ' 10.4
7b3348-2 3.3 1.6 83.1 10.0
.
?b35B1-1 ' 3.7 1.1 83.1 t 0.0
7b2378-2 3.3 1.0 83:1 10.8
7b3211-2 3.2 1.1 83.1 10.5
7b3407-4 3.8 1.8 83.1. 9.3
7b3293-2 3.0 , 1.4 83.0 11.2
7b3298-1 3.~ 1.4 83.0,' 11.0
7b3165-i , 3.2 1.1 83.0 10.5
'
7b35831 3.4 1.1 83.0 10.7
7b2381-2 3.5 0.8 83:0 t0.6
7b3206-1 3.3 1.1 83.0 10.5
7b3346-1 3.3 1.5 83.0 10.3
7b3503-2 3.3 1.0 83.0 t 0.9
7b3526-1 3.2 1:0 83.0 t 0.7
7 b3168-1 3.1 1.0 83.x.. 10.7
7b3429-5 3.4 1.4 82:9 10.2
7b3159-2 3.1 1.1 82.9 t 0.9
v
7b3309-2 3.0 1.3 B2.9~. 11.0
7b3294-Z 3.3 1.5 82:9w t 0.5
7b3335-2 3.4 1.4 82.9: 10.4
7b3497-t 3.5 1.1 82:9 10.6
7b3406-2 3.9 2.0 .82.9 9.2
7b3499-t 3.3 1.1 82.9 10.7
SUBSTITUTE SHEE ~
WO 91/11906 PGT/US91/00917
-46-
s. -.. ...
SABLE S (Continued)
F1EL0 % % .:: % '
oji'.~;:.'.
ENTRY PALMIT1C STEARIC UNOLEiC
:Ol:EIC
7b3317-1 3.4 1.6 ~~e2.9'<10.0
7b2193-4 3.5 1.2 829::; '10.3
7b3357-4 3.6 1.8 82:g~ 9.5
7b3164-2 3.3 1.0 829:v 10.6
7b3235-5 4.0 1.5 828: 9.6
7b3412-2 3.3 1.3 828,. 10.8
7b3221-5 3.6 1.5 828:;: 9.8
7b3424-4 3.5 1.1 828::: 10.8
7b3322-1 3.4 1.7 82.8;=.10.2
7b3499-2 3.4 1.0 82:8'' 10.8
i b3334-13.4 1.4 828 10.1
7b3504-1 3,4 1.1 SZ.g%: 10.5
7b3311-2 3.2 1.6 828;' 10.7
7b3347-1 3.3 ~ 1.6 828;' 10.4
~
7b3331-2 3.3 1.5 828".'.10.1
.
7b3148-1 3.2 1.1 828 10.8
~
7b3238-2 3.8 2.0 ~ B2-7'9.6
7b3424-1 3.4 1.0 827.x' 10.9
1b3295-1 3.2 1.5 82:7:> 10.7
7b3408-2 3.5 1.3 827%' 10.6
7b3081-1 3.5 1.6 827.:,':10.2
7b3345-1 3.5 1.5 827.:: 10.2
7 b3089-23.9 1.7 827'" 9.8
7b3335-1 3.5 1.6 82:7:' 10.1
7b3153-2 3.1 1.1 y 82:T,:10.8
7b3334-2 3.4 1.6 82:~r 10.3
7173179-23.6 0.9 82.7..:10.6
7b2382-5 3.2 1.1 82:7: 10.8 ,
7b3187-1 3.3 1.0 82.7:-.10.6
7b3214-1 3.5 1.0 827.:- 10.1
SUSSTiTUTE SflEE3
WO 91/11906 PGT/US91/00917
~,. J.~
=47-
~<: ~~5~~3~
'~-ABLE :. (Continued)
FIELD % % %.:
ENTRY PALMITIC STEARIC'OLHC LINOLElC
Tb3574-1 3.3 1.5 82':7" 10.7
7b3218-1 3.6 1.0 82.7.." 10.8
7b3147-1 3.1 1.1 82:7 t 1.0
7b3323-2 3.2 1.3 82.7....10.9
7b3182-1 3.2 1.0 827. 11.0
7b3312-2 3.2 1.5 827;'. 10.7
7b3911-1 3.2 1.6 82Z' 10.5
7b3203-1 3.2 0.9 827 10.9
7b3158-1 3.2 1.1 82:7" 10.9
'
7b3303-2 3.3 1.5 82:7 11.0
"'
7b2204-1 3.4 1.1 82.6 t 0.8
7b3293-1 3.1 1.4 826 . 11.0
-
7b2258-2 3.3 1.0 82.6 t 1.3
7b3322-1 3:3 1.4 826'.,. 10.7
7b3149-2 3.2 1.1 826 ~. 10.9
7b3338-1' 3.4 2.0 82.8:. 9.8
7b3218-2 3.3 0.6 82fi., 11.4
7b17-4 3.2 1.4 82:6.: 10.9
7b1970-1 3.6 1.5 82.6 10.1
7b3248-1 4.0 1.3 826 r 10.2
7b3167-2 3.2 1.1 82.6 10.8
~
7b3766-1 3.5 1.1 826... 10.6
7b3359-1 3:8 1.4 825". 10.4
7b3219-6 3.7 1.6 82.5 10.1
7b3212-1 3.3 1.1 82:5:. 11.0
7b3411-2 3.3 1.2 82:5. 11.2
:
7b3208-2 3.3 1.1 82:5. t 1.0
7b3589-2 3.5 1.3 82.5 10.7
7b3173-1 3.3 0.9 82.5. 11.2
7b3329-3 3.3 1.3 825.: 10.8
SUBSTITUTE S~IEEl
WO 91/11906 PCT/US91/00917
b -. >t ;. ~'-4 - : ~ : . -4 8
2t'~5~:~~~ ~~
TABLE 5 (Continued)
~EL~ % % :.Jo'
PALMIT1C STEARIC LINOLEIC
OLEIC
7b3153-1 3.0 1.0 82:5:": 11.1
7b3314-1 3.0 1 82:x': 11.2
_4
7b3588-1 3.5 1.2 82:5''.:10.?
7b1462-3 3.7 1.1 8z5'~- 10.9
7b3777-2 3.2 1.0 82:5':: 11.2
7b3334-1 3.4 1.2 82.5: 11.3
7b2204-2 3.4 1.1 82:5:.: 10.6
7b3242-2 3.8 1.7 8Z5::- t 0.0
7b3323-1 - 3.3 1.4 82.5:': 10.8
7b3797-1 3.7 1.1 825 11.0
Tb2376-2 3.2 1.1 82.5 11.0
'
7b3169-1 3.2 1.1 8Z:4 11.3
7b3147-2 3.3 0.9 824 11.2
7b3319-2 3.3 1.5 82.4 10.9
,
7b2381-4 3.3 0.9 824.- 11.1
.
7b3315-2 3.3 1.8 82:4.. 10.6
7b2378-3 3.2 0.8 82.4' 11.5
.
7b3Z96-2 3.2 1.4 8Z:4~r 11.1
7b3340-1 3.7 1.9 82:4-r 9.7
7b3178-1 3.4 0.9 824. 11.8
7b3356-1 4.0 1.5 824 - 10.2
7b3316-1 3.3 1.6 824: 10.7
7b3496-1 3.3 0.9 82.4"' 11.4
7b3245-1 3.7 1.9 824.: 10.0
7b3297-2 3.2 1.5 82:4:-. I1.0
7b2386-2 4.1 1.5 82.4 9.7
%
7b3592-2 3.6 1.9 ' 82:4 10.2
~;
7b3391-2 3.3 2.0 82.3 10.5
7b3298-2 3.2 1.5 823. 11.2
7b3307-1 3.2 1.4 823 " 11.2
SUBSTITUTE SHEE'i
WU 91/11906 PCT/US91/00917
n
~~$ '~..
'~ ABLE 5 (Continued)
FIELD % % %:.
ENTRY PALMIT1C STF~1RIC LINOLEtC
w OLEIC
7b3261-2 3.4 1.6 82.3 10.7
7b3340-2 3.3 2.1 82.3 9.8
7b3234-2 4.2 1.6 82.3 9.6
7b3585-1 3.3 1.3 823' 11.1
7b3178-1 3.2 0.9 823 11.5
7b3321-2 3.2 1.5 823 11.3
7b2387-2 3.5 1.4 823 10.9
7b3397-2 .3.6 2.1 823 10.0
7b3079-8 3.5 1.6 82.3 10.6
'
7b3322-2 3.3 1.2 82.3 11.2
7b3799-2 3.9 1.6 82.3 10.2
7b17-1 3.1 1.4 82.3 11.5
7b3075-1 3.2 1.2 823 11.5
7b3221-7 3.4~ 1.6 82.2 10.5
7b3199-1 3.6 1.2 822 11.0
7b2385-1 3.7 1.9 82;2 10.2
7b343&1 3.7 1.4 822 10.7
7b3161-1 3.2 1.0 822 11.3
7b3346-2 3.5 1.3 82.2 11.2
7b3117-1 3.9 2.0 822 10.2
?b3309-1 3.2 1.4 822 11.1
7b3108-2 3.4 1.0 822' 11.3
7b3881-1 3.6 1.0 822 11.0
'
7b3091-2 3.9 1.3 82.2 10.9
7b3368-8 4.1 1.9 82.2 9.7
7b3792-2 3.1 1.3 82.2.. 11.4
7b3247-2 3.5 1.4 822. 11.0
7b3324-1 3.4 1.4 82.2 11.0
7b3795-2 3.8 1.2 822 11.0
7b3524-1 3.4 1.1 822 11.4
SUBSTITUTE SHEET
WO 91/11906 . PCT/US91/00917
-50- f-
,,~~
~. '2U5~;435 ,
'~ ABLE S (Cont_:_ued)
FIELD % ~s ~e:. %
ENTA't PALMI?1C STEARIC LINOL-FIC
..._.r OLEIC ,
.--
7b33062 3.4 1.4 82:1 11.2
7b35061 3.6 0.9 82:1 11.3
7b3563-4 3.8 1.2 821 11.0
7b2385-4 3.6 t .5 821 10.6
7b3526-2 3.3 1.1 827 t 1.3
7h3357-3 3.9 t .5 82 7 t 0.5
7b3092-2 4.3 1.9 821 9.g
Tb34243 32 0.8 B21 t 1.7
7b3318-2 3.0 1.0 82_7 t 1.9
7b3313-1 3.3 1.8 82.1 10.9
7b3317-2 3.3 1.6 82.0 t t .2
7b3511-2 4.3 1.8 820 t 0.0
7b3359-2 3.5 1.5 820 t 0.8
Tb3324-2 3.3 ' 1.5 82G t t .3
7b3511-2 4.3 1.8 820 10.0
7b3764-1 3.7 1.1 82fl t t .1
7b3165-2 3.3 0.9 82.0 t t .6
'
7b2001-4 3.4 1.9 82:0 10.5
7b3241 4.0 2.0 82:0 10.5
Z
7b3308-2 3.3 1.3 82.0 12.0
7b3527-2 3.9 1.2 82fl t 0.9
7b3883-3 4.6 1.7 824 9.7
7b3308-1 3.2 1.3 820 12.0
7b3076-10 3.2 1.8 81.9 t t .3
7b33481 3.5 1.6 81.9 t 0.9
7b3341-2 3.4 2.1 81:9. 9.9
7b3619-2 3.5 1.3 81:9 t 1.6
7b3316-2 3.4 1.7 81:9 11.2
7b2386-1 3.8 1.9 81.9. 10.4
?b33151 3.0 1.5 81:9 t 1.6 , .
7b3409-3 3.6 1.6 83:9: 10.5
7b3313-2 3.4 1.6 819 11.4 ,
7b2385-1 3.8 1.9 81.9 10.2
7b3539~t 4.1 1.3 81:9 10.5
7b3414-7 3.7 1.5 81.9 t 0.8
7b3175-1 3.3 1.0 81.9 11.4
SUBSTITUTE SHIEE'~
WO 91/11906 PCT/US91/00917
;r,,:,
~~.;;
-51- ~~aS~,43a~9 ° v'. a , :, .
. r., -.; ,,
Example 6
1988 Breeding Program and Results
These 303 lines were selfed in 1988 and the seed
analyzed for oleic acid production. A total of 352 lines
were selected after the 1988 season which produced seeds
having oleic acid content greater than 80%. The range. in
oleic acid content of these lines was 83.4% to 86.1%. The
linoleic acid content ranged from 7.4% to 10.4% and the
saturated fatty acid content ranged from 4.7% to 5.8%, with
six lines up to 6.5%: The ratio of saturated fatty acids to
oleic acid ranged from 0.055 to 0.0?0, with three lines up to
0.075. Twenty-two of these .lines produced seeds having an
oleic acid content of greater than 85%. The mean tempera-
tures for July and August were 73.7'F and about 70.6'F,
respectively. The results are shown in Table 6.
WO 91/11906 PGTfUS91/00917
,:;,:
?i~S~:~a~~ ~ : . -52-
:ABLE o
F1EL0 '
ENTRY ,v % ~a''....
_ _PALMITIC OLCaC LINOLEIC
STEARIC
16:0 18:0 18:1 t 8:2
~..
M 88-5293-13.5 t .3 86:'1::.::7.6
m
M88-5360-13.6 1.3 85:4: 7.9
m
MB8-5486-13.6 t .3 85:4':'.8.0
m
MB8-5294-t3.6 t .2 853':'.8.4
m
M88-5485-tm3.7 1.2 85:3:.8.0
M 88 5383-t3.8 t .3 85:3 7.9
m ~
M 88-5359-13.6 t .2 85:2:;:8.2
m ,
M 88-5318-13.9 t .5 85:2;:.7.6
m
MB8-5314-t3.9 t .4 85:2:::7.7
m
M88-5183-tm3.8 t.3 85.2: 7.8
M 88-5360-t3.6 t .2 85:2 8.2
m ..
M88-5271-tm3.8 t.2 85:2. 7.9
MB8-5297-tm3.9 t.6 85;1' 7.4
MB8-5297-tm3,8 1,6 85a. 7.4
M88-5198-1m4.0 1,4 8fr:0 7.9
'
M 88-4249-13.5 t .2 85.0:;,8.8
m
M88-5268-13.9 1.5 85.0:::7.8
m
M 88-4341-13.7 - 1.2 85:0 8.4
m
M88-5359-13.7 t .2 85:0, 8.6
m .
MB8-5197-1m3.8 t.3 86:0: 8.3
M88-5374-t3.8 t .3 85.0 8.1
m .
M88-4426-13.5 1.2 85.0 8.8
m
MB8-5317-1m3.8 t.2 84.9-:,8.5
MB8-4431-1m3.7 1.2 84.9 8.5
88b-5514-13.8 1.2 84.9: 8.3
88b-5646-13.7 1. 8a.8-:8.8
t
88b-5588-13.8 1.5 84~:8.'.:~8.2
MB8-5309-2m3.6 t.5 84.8: 8.5
M88-5190-1m3.8 7.3 84.8..8.3
M88-4412-13.7 1.2 84.8: 8.5
m
MB8-5387-1a.0 1.5 84.8 8.2
m
M B8-5273-13.7 ? .3 84.8 8.5
m
M88-5308-2m3.6 t.2 84.8 9.0
M88-5310-t3.8 1.2 84.8 8.6
m
M88-5360-4m3.6 t.3 84.8 9.9 .
WO 91/11906 PGT/US91/00917
~:,~i:
.. ~. ;.; .~. ,~.'' ~ _ _ ; .
.t~.~Jr~.~~~
TABLE 6 (Continues)
FIELD
ENTRY !o !e !~
PALMIT1C OLEIC LINOLEIC
-... STEARIC
.....
16:0 18:0 18:1 18:2
M88-5217-1 m 3.7 1.3 84:8: 8.5
M88-5370-im 3.9 t.4 84.8 7.7
M88-5298-i m 3.8 t .2 84.8 8.9
M88-5372-1 m 3.9 t .6 84.7 7.7
M B8-4732-t m 3.7 t .4 84:7 8.2
M88-5288-t m 3.6 1.6 84:T..".:8.6
88b-5603-1 3.8 1.2 84.T.''8.5
M 88-5189-t m 3.8 t .2 84.7 8.8
~
88b-5529-1 3.8 t .5 84.7 8.4
M88-5401-1m 3.8 1.8 84.7 7.9
MB8-4993-t m 3.9 t .4 84.7' 8.6
M88-5392-1 m 3.8 t .6 84.7 8.3
M88-5373-t m 3.7 1.3 84.7 8. t
MB8-4823-1 m 4.2 t .6 84.7 8.0
M88-5292-t m 3.8 1.5 84.7 8.3
88b-5593-t 3.9 1.6 84:7 8.2,
M88-5302-2m 3.6 1.6 84 8.5
6.
MAS-4502-1 m 3.6 t .3 84.6 9.0
:
M88-5291-tm 3.9 t.6 84.6 8.t
M 88-4428-1 m 3.6 t .3 84.6 8.7
MB8-4256-tm 3.8 t.t 84.6 8.6
M88-5304-3m 3.6 t.2 84.6 9.0
M88-5364-1 m 3.7 t .4 84.6 8.4
hdBB-5395-t m 3.6 t .5 84.6 8.7
M 88-4797-t m 3.8 t .5 84.5. 8.7
MB8-5228-t m 4.0 1.5 84.5 8.4
M88-5301-tm 3.7 t.6 84.5 8.6
MB85t37-tm 4.0 t.4 84.5 8.6
M88-4731-1 m 3.9 t .5 84.5 8.0
MHS-5193-1 m 3.9 1.3 84.5 8.5
MB8-5303-1 m . 3.8 t .7 84.5 8.3
88b-5641-1 3.7 t .2 84.4 9.0
M88-5272-tm 3.9 t.6 84.4 8.5
M88-4743-t m 3.9 t .3 84.4 8.6
M885382-tm 3.9 t.2 84:4 8.6
MB8-4414-tm 3.9 t.2 84.4 8.6
M88-5202-t m 3.7 t .2 84.4 ?.2
WO 91/1906 ~ PCT/US91%00917
r::
~i~ 'J,r.~'~ .~~a~l -54-
TABLE 6 (Cont~.z~~ec)
FIELD
ENTRY % % ~ 'v
--~.,... .-.PALMITIC OLEICM! _INOLE1C
STEA_RIC__ w
-.__
# 16:0 ; 8:0 _t t _3:2
_ 8:1 _ -
w
M88-4264-13.4 1.2 84.4 _
m 9 1
88b-6295-13.7 t .6 84.4.8.8
M88-5204-t3.7 t .2 84:4 9, t
m
M88-5478-t3.7 t .2 84.4 8.8
m
M88-4437-t3.8 t .3 84.4 8.7
m
M88-5326-14.1 t .3 84.3 8.6
m
M88-5388-im3.9 1.4 84.3 8.7
M88-4608-13.8 1.5 84.3 8.5
m
M88-5365-1m3.7 1,5 84.3 8.4
M88-5227-1m3.9 t.6 84.3 8.7
M88-5361-t3.7 t .2 84.3 9.1
m
M885086-t 3.9 1.5 84.3 8.9
m
M8844t5-tm3.9 1.3 84.3 8.4
M88-5282-13.5 1.2 84.3 9.3
m
MB8-5082-1m4,0 1.3 84.3 9.2
Mg8-5184-tm3,8 1,4 84.3 8.9
M88-4404-13.7 t .2 84.3 9.0
m
MB8-4729-1m~ 4.1 1.3 84.2 8.2
M88-5195-14, t t .3 84.2 9. o
m
MBS-4416-13.6 t .2 84.2 9, t
m
M88-5375-14.0 t .6 34.2.8.2
m
M88-5362-13.6 t .3 84.2 ~, t
m
MBB-5270-t3.8 1,4 84.2 3.1
m
88b-6019-53.8 t.0 84.2 3,8
M88-5258-t3.8 1.7 84.2 9.8
m
MBS-4421-tm3.8 t.t 84.2 .2
n
M88-5246-14.0 t .6 84.2 8.7
m
MB8-5201-tm3_7 1.3 84:2 8.8
M88-5360-3m3.8 t .2 84.2 8.9
M88-5313-1m3,6 1.2 84.2 9.4
88b-5504-14.0 t .2 84.2 3.9
MB8-5359-4m3,7 1.2 84.2 9.6
MB8-5192-13,g 1.2 84.2 9, t '
m
M88-4435-t3.7 1.2 84.1 9.1
m
MB8-5386-tm3,g t.2 84.1 ?.2
MB8-4990-13.9 1.5 84.1 ~ , t
m
MB8-5203-13.7 t .2 34.1 3.4
m
88b-6019-13.7 1.5 84.1 3.0
WO 91/11906 PGT/US91/00917
;:.'
~ ~.~ . ~ ~ r , 0.,'...i :n,t;~
f i
.4p5~43S
.'ABLE 6 ( Ce:: ~_--;:ec )
FIELD
ENTRY .~o o .~e '~o
PALM_IT_IC__S_TEARIC_ OLEICLINOLEIC
~ 1
---
# 16:0 18:0 18a 18:2
M88-5209-1 m 3.9 t .3 84.1 8.9
M88-4248-1 m 3.7 t .1 84.1 9.2
M88-4423-t m 3.6 t . 84.1 9.5
t
MB8-5368-t m 3,7 t .3 84. 9.0
f
M88-4208-1 m 3,7 t .3 84.1 9.0
88b-6014-3 3.6 t .4 84.Z 9.3
88b-5499-1 3.8 1.3 84a 8.7.
MB8-5354-t m 3.8 t ,5 84.1 0.9
MB8-4402-1 m 3.5 t .2 84.1 9.2
M88-5400-t m 3.9 t .8 84.0 8.3
M885078-1 m 4.0 t .5 84.0 9.4
MB8-5360-2m 3.5 t . 84.0 9.8
t
M88-4197-1 m 4.0 t . 84.0 8.9
4
MBS-5359-3rri 3,8 t.2 84.0 9.4
M884250-t m 3.6 t .0 84.0 9.7
M88-5385-t m 4,2 t .2 84.0 8.7
M88-53241 m 3.7 t .3 84.0 9.3
M88-5381-tm 4.0 t.5 84.0 8.4
M88-5269-t m 4. t .4 84.0 8.8
t
M88-5377-t m 3.7 t .2 84.0 9.4
88b5650-1 3.9 t . 84,0 9. t
t
M88-5376-1 m 3.9 t .5 84.0 8.7
,
M88-4247-1m 3.7 t-t 84.0 9.6
MB8-5371-tm 3.7 t.3 84.0 8.9
M88-5083-2m 3.8 t .5 84.0 9.2
M88-5321-t m 4.0 t .5 84.0 9.0
M88-4446-1m 3.7 t.t 84.0 9.5
88b-5660-1 3.9 t .4 84.0 8.9
M88-5284-1 m 3.7 t .3 83.9 9.8
M88-5281-t m 3.8 t .2 83.9 9.7
88b-5509-t 3.9 t . 83.9 9.2
t
MB8-5479-1 m 3.8 t ,3 83.9 9.3
M B8-5133-t m 4.0 t .3 83.9 8.9
88b-6019-4 3.7 1.5 83.9 9.1
M88-5136-tm 4,0 t.3 83.9 9.0
M88-4122-t m 3.7 t , 83.9 9.5
t
MB8-5214-tm 4.0 t,2 83.9 9.4
88b-So37-t 3.8 t .5 83.9 9.2
WO 91/11906 PGT/US91/0091?
_.. , - -56-
205~435
ABLE o' (Con~~nueci)
FIELD
ENTRY
PA__LM__ITiC OLEICLINOLEIC ,
STEARIC
'-___
~
__-
# 16:0 t 8:0 18:1 18:2
MB8-4424-tm3.6 1.3 83:9'9.5
M88-5366-13.8 1.3 83:9"'9.0
m
M88-4737-tm3.7 1.3 83:9 9.2
M88-5476-tm3.8 t.5 83.9:.8.7
M 88-5305-2m4. t t . 83.9 8.7
7 ~
-
MB8-4445-tm3.8 1.3 83.9.9.1
M88-5306-t3.8 1.5 83.9 9.4
m ..
88b-5519-13.9 1.4 83.9.8.8
MB84452-t 3.7 t .2 83:9:9.5
m
rd88-5379-13.9 1.4 83:9 8.7
m
M88-4450-1m3.7 t.3 83.9 8.8
88b-6053-13.8 1.6 83:9'8.9
M88-5477-t3.7 1.3 83.9 9.2
m .
88b-5655-13.8 1.1 83.9.9.4
M88-4534-t3.7 t .5 83.9 9.3
m .
MB8-4596-13.7 1.6 83.8 9.2
m .
M88-5132-14.2 1.6 83:8 8.7
m :.
MB8-4448-1m3.7 1.1 83.8 9.3
88b-6017-33.6 1.6 83:8 9.5
M88-4345-13.9 1.8 83:8 8.7
m
88b6018-5 3.8 t .5 83.8 9.3
M88-5185-13.9 t .7 83.8 8.9
m
M88-4401-1m3.6 1.4 83.8.9.2
~88b-6220-13.5 1.8 83.8 9.3
SSb-6023-23.6 1.4 83.8 9.5
MB8-5187-13.8 t .3 83.7 9.7
m
88b-6013-53.9 1.S 83:T~9.4
~
M88-5325-t4.1 1.6 83:7 9.2
m '
88b-6014-43.7 1.5 83-7 9.5
MB8-5265-t3.8 1.6 83.7 9.3
m
88b-6021-33.6 1.4 83.7 9.7
M88-5135-1.s. t .5 83.7 8.9 .
m t
88b-6025-33.5 1.6 83.7 9.6
M88-5367-13.7 t .3 83.7 9.2
m
88b-6035-23.8 1.5 83.7 9.3
88b-6359-13.7 1.4 83.7 9.6
M88-4733-tm4.0 i.3 83.T 9.0
WO 91/11906 PGT/US91/00917
-57-
~~sa~r
TABLE 6 (Cont?nued)
FIELD
ENTRY % % % .o
PALMIT1C OLElC LINO~EIC
STEARtC
._
# 16:0 18:0 18:1 18:2
~
M88-5316-1 m 3.9 1.2 83:1' 9.7
~
M88-4354-1 m 4.8 2.1 83:7 8.0
M88-5384-1 m 3.6 1.3 83:7 9.7
M88-4582-1 m 3.5 1.5 83.7 9.5
88b-6178-1 3.6 1.5 83.7 9.4
88b-6462-1 3.6 1.7 83.7 9.4
88b-6170-1 3.6 1.5 83:T' 9.5
ME~84457-1 m 3.9 1.5 83:7:'9.3
MBS-5380-1 m 3.7 1.3 83.6. 9.5
88b-64691 3.6 1.6 83.6.'9.4
M B8-4201-1 m 3.9 t .4 83.6 9.4
.
88b-6458-1 3.8 1.7 83.6 9.1
M~8-5327-tm 4.1 t.2 83.6 9.5
88b-6341-1 ~ 3.6 1.5 83.6 9.7
MBS-5231-1 m 3.7 , 1.6 83:6: 9.5
88b-6257-t 3.8 1.5 83:6 9.6
. M88-5216-1 m 4.0 1.3 83.6 9.2
88b-6052-1 3.8 1.5 83:6'.9.4
M88-5425-1 m 4.4 1.7 83.6 8.5
88b-6121-1 3.6 1.6 83.6. 9.6
M88-5397-1 m 3.9 t .4 83.6 9.5
88b-6027-3 3.6 1.S 83.6 3.6
MI38-5357-1 m 4,0 1.4 83.6 9.4
88b-6025-2 3.6 1.5 83.6 9.5
88b-fi481-1 3.7 1.5 83:6 9.5
88b-8020-4 3.5 1.5 83.6 9.6
M 88-5346-1 m 4.1 1.4 83.6 9.
t
88b-6015-5 3.8 1.6 83.6 9.4
Mt38-5222-1 m 3.9 1.2 83.6 t 0.0
M884351-1m 4.4 1.9 83.6 8.4
M88-5414-1 m 4.0 1.4 83:6 9.4
MBS-4798-1 m 3,g 1.4 83.6 9.5
MB8-5348-1 m 4.0 1.4 83.6 9.4
MB8-4436-1 m 3.7 1.3 83.6 9.5
MBS-5243-tm 3.8 1.6 83.6 9.3
M88-4405-1m 3,g 1.2 83.6 9.6
MBS-5369-1 m 3,7 1.3 83.6 9.2
WO 91/11906 PGT/US91l00917
-58-
~~3LE 6 (Continued?
FIELD ,
ENTRY % % f.:. ,e
PALMITIC OtHC LINOLEIC
STEARIC
# 16:0 18:0 l8a 18:2
M88-5146-1 m 3.6 1.2 83.6' 9.9
88b-6473-1 3.7 1.7 83.6 9.0
-
M88-4549-1 m 3.8 1.4 83.8 9.7
M88-4123-1 m 3.9 1.2 83:5 9.4
88b-6471-1 . 3.9 1.7 83.5. 9.2
88b6479-1 3.7 1.6 83.5 9.6
MB8-5434-1m 4.1 2.1 835 8.5
M885407-1 m 4.2 t .5 83.5. 9.2
.
88b-6036-5 3.4 1.4 83-5 9.9
M88-5394-1 m 3:7 1.5 83.5 9.6
88b-6028-3 3.7 1.5 93.5 9.4
88b2555-2 3.9 1.6 83.5 9.4
88b-6022-5 3.7 t .6 83.5 9.6
M88-4544-1 m . 3.6 1.4 83.5 9.9
88b-6017-1 3.9 1.5 83.5 9.4
88b6106-1 3.8 1.5 83.5 9.5
88b-6011-3 3.7 t .6 83.5 9.5
,
88b-6115-1 3.7 1.5 83.5 9.7
88b1798-1 m 3.6 1.4 83:5 9.8
M88-48431 m 4.3 1.6 83.5 9.1
M88-5278-1 m 3.7 1.1 83.5 t 0.2
M88-4738-1m 3.7 1.2 83.5 9.7
M88-5175-1 m 4.3 t .4 83.5 9.1
MBS-4366-1 m 3.7 1. 83.5 9.9
t
M88-5029-1 m 3.9 1.5 83.5 9.3
M88-5393-1 m 3.8 1.4 83.5 9.6
.
88b-6435-1 3.9 1.5 83.5 9.6
M88-5378-1 m 3.8 1.1 83.5 9.4
88b-6025-4 3.6 1.6 83.5 9.6
M88-5363-1 m 3.8 1.3 83.5 9.4
88b6015-2 3.7 1.5 83.5 9.7
M88-5353-1 m 4.0 1.5 83:5 9.4
M88-5322-1 m 3.9 1.4 83:5 9.7
88b-6485-1 &2 3.7 1.6 83.5 9.6
M88-5154-1 m 3.7 1.2 83.5 10.4
88b-6464-1 3.7 1.6 83.5 9.5 .
88b-6033-5 3.7 1.6 83.5 9.5
WO 91/11906 PGT/US91/009I7
-59-
TABLE 'o (Continued?
~.::~5x~~~
FIELD
ENTRY % % l~o's' o
PALMITIC OLEICLINOLEIC
_... STEARIC
----
t 6.0 t 8.0 18i!'.~ 8.2
88b-6131-1 3.6 1.6 83:5:.9.5
88b6010-5 3.9 t .5 83:5':9.4
88b-6091-1 3.6 1.3 83.5 3.7
M88-5359-2m 3.7 t.t 83.5 '0.3
M88-5267-t m 3.8 1.7 83.5 9.5
88b-60184 3.8 1.5 83.5 9.7
88b-6323-1 3.6 1.3 83.5 9.9
88b-6027-1 3.7 t.6 83.4 9.7
M88-5358-1m 3.9 1.2 83.4 3.7
88b-6383-t . 3.6 1.5 83.4 9.9
M88-5398-tm 4.t 1.6 83.4 9.2
88b60233 3.9 1.7 83.4 9.a
M885423-t m 4.2 r.6 83.4 9.0
88b-6302-1 ~ 3,7 t .4 83.4 9.8
M88-5480-t m 4,0 1.4 83:4 9.5
88b6130t 3.6 1.5 83.4.9.8
M88-5178-1 m 4.3 1.5 33: 8.9
4
M68-4203-1 m 3.8 1.3 83.4 9.5
M88-5241-1m 4.1 1.5 83:4 9.6
M884275-1 m 4.6 t .6 83.4 8.6
M88-5245-1 m 3.8 t .7 83:4 9.5
M98-4403-1 m 3.7 t .0 83.4 ~.9
M88-5253-1 m 4.0 1.6 83.4 ~.5
M88-4641-1 m 4.0 2.5 83.4 8.2
M88-52661 m 3.8 t .5 83.4 3.9
M88-4510-t m 3.6 t .4 83.4-0.0
M88-5283-1 m 3.9 1.3 83.4 9.8
88b-6426-t 3.6 1.2 83.4 ; 0.2
M88-5328-t m a. t t .4 83.4 9.6
. 88b-6475-1 3.? t .4 83.4 9.8
88b-6002-2 3.8 1.6 83.4 9.8
88b-6051-t 3.7 1.4 83:4 9.7
88b=6010-4 3.9 1.6 83.4 9.3
88b-6179-1 3.7 1.6 83.4 9.8
88b-6011-4 3.8 1.6 83.4 9.5
WO 91/11906 PGT/US91/00917
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PALMITiC STEARICOLEIC~INOLE:C
-.~..~..__ --_---_-~~~~..__~ ~.._-_.
# t6:0 t8:0 18:1 t8:2
Ms8-4513-1 m 3.7 ~ .5 83.4 9.9 ,
88b-6012-t 3.6 1.5 83 9.7
4
M88-4393-t m 3.5 ' .2 83.4 ~ 0.0
88b-6012-3 3.0 t .5 83.4 9.7
M88-4417-t m 3 ~ t .2 83.4 9.5
88b-6013-2 3.8 t .5 33.4 9.6
88b-5548-t 4. i t .7 83.4 9.2
88b-6014-5 3.7 t .5 83.4 9.9
88b-6127-1 3.7 t .5 83.4 9.9
88b-6015-4 3.8 t . 6 83.4 9.5
M88-4589-1 m 3.5 1.5 83.4 9.6
S8b-6016-4 3.8 t .5 83.4 9.5
88b-63641 3.7 t .3 83.4 .9
88b-6449-t 3.6 :.8 33.4 9.a
M88-4453-t m 3.6 t .2 83.4 : 0.0
88b-601 8-t ' 3.5 t .7 33.4 9.8
WO 91/11906 PGT/US91/00917
-61- 5~-4~ a
.y;~ ,9~i1°~
.. r.-. ?~: ~: . .
These 352 lines were selfed in 1989 and the seed analyzed for
oleic acid production. Slight changes seen in the oleic
acid content of the progeny with some lines approaching 87%.
The mean temperature for July and August was 75.6'F and about
70.8'F, respectively. In order to increase the oleic acid
content to greater than 87% and up to 90%, the previously
obtained lines are intercrossed to derive new lines. These
new lines produce seed having an oleic acid content of up to
90%.
Example 7
Uniformity of Oil Production
Several lines were followed between 1985 and 1988 to
demonstrate the stability and inheritability of the high
oleic acid trait. These lines were grown and the seed
harvested and analyzed each year. The results are shown in
Table 7.
TABLE 7
Fatty Acid Percentages of
Various Oleic Breeding Lines (Over 3 Years)
Percentagre
Breeding Saturates Oleic
Line Year 116:0_) 8:0 18:1
1 88 3.6 1.2 85.2
87 3.3 1.0 82.5
86 3.0 1.1 83.8
AVERAGE ___
___
________ ____ 3,3 ______ 1.1 ____
___ g3,8
2 88 3.8 1.3 83.5
87 3.3 0.9 82.5
WO 91/11906 PGT/US91/0091?
2~5143~
86 3.2 1.0 82.7
--_ __________.
AVERAGE
-_ ___ 3.4 -_____ 1.1 ___ g2.9
-___
3 88 3.8 1.1 83.5
87 3.0 0.7 84.7
86 3.3 1.1 83.7
AVERAGE --__ ______________ 3.4 ______ 1.0 -__ 84.0
--__
4 88 3.8 1.6 84.7
87 3.3 0.6 82.6
TABLE Con't.)
7 (
Fatty
Acid
Percentages
of
Various Oleic Breeding Lines (Over Years)
3
Percentacre
Breeding Saturates oleic
_ Line dear (16:0 1 18:1
86 3.5 1.4 84.0
_ .
____ __
_
AVERAGE ---- _ . -_____ 1.2 ___ g3.8
. _ 3.5 ---_
__
88 4.2 1.6 82.2
87 3.5 1.3 82.7
86 3.3 1.4 80.6
AVERAGE --__ ____-_________ 3 ___-__ 1
7 4
. --__ ___ g1.8
.
6 88 4.1 1.5 82.3
87 3.2 1.2 83.0
86 3.4 1.4 82.7
. __ .
3
6
AVERAGE --__ _____ _ ____ ___ g2.7
____ _ _ 1.4 -___
.
7 88 4.1 2.3 81.2 '
87 3.5 1.6 83.2
85 3.6 2.7 81.1
AVERAGE -___ ______________ 3.7 -_____ 2.2 _-_ g1.8
____
WO 91/11906 PCT/US91/00917
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8 88 4.3 2.3 80.3
8? ~ 3.5 1.6 81.3
85 3.6 3.0 80.5
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___ _-__ 3.8 -_____2.3 --_ __-- g0.7
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87 3.5 1.0 84.2
85 3.3 1.2 82.8
AVERAGE --_ -_-_--____
_ __-- 3.5 __-___1.2 -__ -___ 83.6
Example 8
Breeding Pedigree of Safflower Variety Montola-2000
Montola-2000 was derived from an individual F6 plant
selection from the 1982 cross of a female parent resulting
from the multiple cross Frio/Sidney Selection 87-14-6///
79AZ9543-1/[S-304/(White US-10/UC-1//Oleic Leed, White
S-208//[UC-1/(White US-10/UC-1//Oleic Leed), White
S-208]/[Th-5/~. nitidus//o141-1]] With a male parent
resulting from the multiple cross White US-
10/UC-1//[S-304/(US-10/UC-1//Oleic leed), white s-zo8]. From
the initial crosses of White flowered US-10/UC-1 and Th-5/ _C.
't dus, a total of 19 crosses were made in the breeding of
this cultivar. Individual plant selections were made during
the F2, F3, F4, F5 and F6 generations. Fatty acid determina-
tions were made on seed in the F6, F7 and F8 generations to
verify the cultivar produced oil low in saturates and very
high in oleic acid (greater than 80%).
The multiple crosses were made in the following scenar-
io.
Female Parent
cross 1 - Frio x Sidney Selection 87-14-6 (A)
- White US-10 x UC-1. (B)
- Th-5 x C. nitidus (C)
cross 2 - (B) progeny x Oleic Lead (D)
- (C) progeny x o141-1 (E)
WO 91!11906 PCT/US91/00917
2051,45
c~:i.°
. ~, ..
.. .. -64-
cross3 - (D) progeny S-304 (F)
x
- (D) progeny UC-1 (G)
x
cross6 - (G) progeny (E) progeny(H)
x
cross10 - (F) progeny (H) progeny(I)
x
cross11 - ?9A29543-i (I) progeny(J)
x
cross13 - (A) progeny (J).progeny(Female Parent)
x
Male Parent
cross1 - US-10 x UC-1(K)
cross2 - (K) progeny Oleic Leed (L)
x
cross3 (L) progeny S-304 (M)
- x
cross4 White US-10 UC-1 (N)
- x
cross5 (N) progeny (M) progeny(Male Parent)
- x
The objective description of variety Montola-2000 is as
follows, in accordance with characteristics for a plant
variety application.
Maturity - 120 days (Midwest location)
Plant height at maturity - 43 cm
Flower color - Yellow (fresh);
Light orange base (wilted)
Spines on involucral bracts - present at tip and
along margins: 2.2 mm in length numbering 15
with a spine index of,33 (class 2 = 21-40)
Heads - 25 mm diameter of primary head:
1-10% seed shattering
Seed - White/white with blotch (4/100); normal
hull: 4 mm wide, 7 mm long, 34 g/1000 seeds
Seedling vigor - 8 nodes; 21 cm soil surface to tip
Cold resistance - Rosette: -5'C;
Bolting: 0'C;
Flowering: 5'C
Disease - moderately susceptible to Alternaria leaf spot
and Alternaria bud rot
Hull - 44.6%
WO 91/11906 PGT/US91/OQ917
~~ ' a ,
t~':,i; Y~. ~', ~ t, s
-65-
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Protein - 23.0%
Oil - 44.0%
Todine - 84%
Saturated Acids - 5.5%
Oleic acid - 81.7%
Linoleic acid - 11.4%
The fatty acid profile of variety Montola-2000 was
examined in 1987, 1988 and 1989. The results are shown in
Table 8.
TABLE 8
Montola-2000 Safflower Fatty Acid Profile
1987 TO 1989
Seed Palmitfc Stearic oleic Linoleic
ce ea .~ 16601 8:0) 1 :l 8:2
87b3797-187 3.7 1.1 82.5 11.0
87b3797-287 3.9 1.2 81.3 12.0
AVERAGE -__________ 3 __ 1
- g ___
, .2 ___ - 81.9 __ 11.5
--
88b5623-188 4.4 1.6 81.9 10.7
88b5628-188 4.2 1.9 81.2 11.1
88b5633-188 4.2 1.5 82.9 9.8
TABLE 8 (Con't.)
Montola-2000 ower FattyAcid
Saffl Profile
1987 TO 1989
WO 91/11906 PCT/US91/00917
. . ..
. ~Q51.~ ~~ - _.
. 66-
-: ,
Seed Palmitic Stearic Oleic Linoleic
Source Year (16:0) (18:01 18: f18:2Z
88b5347-1 88 4.2 1.5 82.5 10.3
88b5348-1 88 4.0 1.4 83.6 9.4
88b5350-1 88 4.3 1.7 82.0 10.6
AVERAGE ------
--
_ --- 4.2 -- 1.6 - 82.4 _- 10.3
--- _-- --
Breeders
Seed-1 89 4.2 1.5 80.0 12.8
Breeders
Seed-1 89 4.1 1.5 81.6 11.3
Breeders
Seed-1 89 4.2 1.3 80.6 12.7
Breeders
Seed-1 89 4.3 1.3 81.4 12.2
Breeders
Seed-1 89 4.2 1.5 80.5 12.6
AVERAGE ___----_-_ 4.2 _- 1.4 - 80.8 -_ 12.3
-_ --- --- -_
Variety Montola-2000 was compared with several other
varieties commonly grown in Montana and North Dakota. The
results are shown in Tables 9-19.
WO 91/11906 PGT/US91/00917
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WO 91/11906 PCT/US91/00917
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WO 91/11906 PCT/US91/00917
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WO 91/11946 P(.'f/US91/00917
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WO 91/11906 PCT/US91/00917
-78_
A total of 2500 seeds of variety Montola-2000 high oleic
are concurrently being deposited with the American Type
Culture Collection, Rockville, Maryland. The deposit is
assigned number 40751. A plant variety protection applica-
tion is also being concurrently filed.
A total of 2500 seeds of a high linoleic acid oil
content are deposited with the American Type Culture Collec-
tion and designated as Morlin.
Example 9
Breeding Pedigrees of
Representative Safflower Varieties
The following high oleic acid safflower varieties were
obtained by the described breeding pedigree.
(1) 8885293-1
female parent:
[(Biggs/PII95895//C.L.1653/87-42-3)//AC-1]/79AZ2603 crossed
with
r~tale~arent: [(Frio/87-42 3 87-42 3 AC-1
- // - / )/79AZ29379-7]
(2) 8885360-1
female parent: (AZ9322//87-14-6/S-208)//AZ2698/012-251-3-6
crossed with
male parent: (87-42-3/AC-1//AZ2698/012-251-3-6)///[(87-
14-B/N-10//87-42-3/AC-1)//(87-42-3/AC-1//AZ2698/012-
251-3-6)]//white 80 ours. pigmentless
(3) 8986051-1
female parent: [(AZ2698/012-251-3-6//87-42-3/AC-1)// white 80
nurs. pigmentless]//UCD1982-620/Hartman///[(87-
42-3/AC-1//AZ749-29)//79AZ2603] crossed with '
male parent: S-317
(4) 89B6594-1
WO 91 / 11906 PCT/US91 /00917
'°:
-79- G~~~~y
female barent: 87-42-3/AG-1//[(87-14-B//87-42-3/AC-1)//
OL35-2/PCM-1] crossed with
male-parent: 79AZ9386-1
(5)
female parent: [87-42-3/AC-1//[(87-42-3/AC-1//87-42-
3/AC-1)//OL35-2/PCM-lJ]///[(87-42-3/AC-1//88/74-2/N-
10)//Mex.Dwarf-2//OC35-2/PCM-1)]//white 80 nurs. pigmentless
crossed with
male_parent: (87-42-3/AC-1//AZ746-29)//79AZ2603
Examples 10-13 are directed to the development of high
linoleic acid varieties.
Example l0
1985 Breeding Program and Results
The top 316 lines for linoleic acid production were
selected from a group of 6000 crosses which were made in
1985. The 1985 lines produced from 83.2% to 89.2% linoleic
acid in the seed. The oleic acid content ranged from 3.9% to
8.8% and the saturated fatty acid content ranged from 487% to
8.0%. The ratio of saturated fatty acids to linoleic acid
ranged from 0.063 to 0.096. The mean temperatures for July
and August were 71.4'F and 66.3'F, respectively. The results
of 192 representative lines are shown in Table 20.
WO 91/11906 PGT/US91/00917
-80-
. ;. ,~,~~~:~'ar'. ~ TABLE 20
SAMPLE %
# %
%
m PALMITYC OLEIC LIN'OLEIC
STEARIC
..w~uww,.~w.mr"wm.,mwmrw r"wwwrrrrrw..~rrrrrmwwrw-wrrw~wowrrr-m
3595
3
4
. 3.9 89.2
3576 1.4
5.3 87.2
3605 4.3 1
3
. 5.4 86.8
885 3.8 1 ~
6
. 6.5 86.1
3598 4.8 L3
6.1 85.7
718 4,0 1.7 7.2 85
6
886 4.2 L7 6 .
9 85
5
56S 4.6 1.5 . .
6.5 85
5
882 4.1 L8 7.3 .
2537 85.5
4.$ 1.5 6.0 85.3
2723
6.3 85.2
2536 4.8 5
1. . 6.0 85.2
3449 5 1
2 8
. . 5.9 85.1
2538 4.9 I.6 6 85
2 I
3450 5.2 1.8 . .
6.0 85
0
2812 5.4 1.6 S.8 .
85
0
2656 5.4 1.6 6.0 .
$5
0
2811 5.5 1.7 5.8 .
84
9
3447 5,2 .
L8 6.1 84
9
2657 5.6 1.5 6.Z .
$4
9
26SS 5.5 1.6 6.2 ,
84
9
2783 5.1 1.6 6.s .
s4
9
2541 5.0 1.5 6.1 .
84
9
3 i 5.4 1.6 6.1 .
32 84
8
3590 4.7 1.8 6.9 .
84
8
1246 4.8 i.8 7.I .
84.8
3956 5.4 1.8 6.0 84.8
3596 4.9 L7 6.7 84.$
3448 5.2 1.8 6.4 84.7
3446 5.2 1.8 6.3 84
7
3606 4:I 1.5 7.8 .
84
7
2784 . 5.3 1.6 6.5 .
' 84.7
2810 5.2 1.6 6.4 84
7
2782 5.1 1.6 6.8 .
84.7
3452 5.3 1.9 6.3 84.b
1254 4.7 1.7 7.3 84.6
2833 4.7 1.6 7.2 84.6
458 3.9 1.9 8.2 84.6
3451 5.2 1.9 6.4 84.6
3905 5.0 1.9 6.6 84.6
3131 S.1 1.7 6.8 84.6
1245 4.9 1.9 7.1 $4.5
1252 4.7 1.6 ?.6 84.5
3453 5.4 1. $ 6.2 84.5
~
2316 4.9 1.5 7.7 84.5
2688 5.1 1.7 7.0 84.5
2625 4.5 2.2 7.0 84,4
1256 4.7 2.1 7.3 84.4
SL3'~~T9~Li~~ ~, .=
WO 91/11906 PGT/US91/00917
/y:,,
-81- ;'~,~~ 'J~.
TAaLi: (Continued)
20
SAMPLE # % % %
PALMxTIC STEARIC OLEIC LINOLEIC
..wwr..wwww..wwrwrwwwwwrw..wwwwwwwrw wwwwrww wwrwwrwwrr
3368 5.4 2.1 7.2 83.4
2017 3.5 2.1 7.5 83.4
3S 17 S.6 1.6 7.5 83.4
2153 5.1 i.S 8.8 83.4
6x 3.1 1.9 8.0 83.4
2512 5.1 1.6 8.0 83.4
2218 5.1 1.9 8.1 83.4
2866 5.4 I, 8 7.7 83.4
3511 5.5 1.7 ?.6 83.4
201S 5.5 2.2 7.6 83.4
2105 5.2 1.8 8.2 83.4
1853 6.0 1.8 7.4 83.4
2162 5.4 1.8 8.I 83.4
2382 5.5 1.8 ?.9 83.4
3256 5.1 1.5 8.1 83.4
3383 5.4 1.7 7.6 83.4
3875 5.3 1.8 7.6 83.4
1$49 6.1 1.? 7.4 83.4
1$47 6.0 1,7 7.5 83.4
4333 5:0 L7 8.3 83.4
92I 4.7 1.6 8.6 83.4
2677 5.0 1,8 7.7 83.4
714 5.2 1.7 8.0 83.4
1674 5.1 1.9 8.0 83.4
2513 5.1 1.6 8.2 83.4
3496 5.3 Z.1 7.3 83.4
3654 5.5 1.5 7.7 83.4
1019 S.0 2.0 7.7 83.4
4682 5.1 1.6 8.2 83.4
3308 3.3 2.0 7.5 83.3
3S1S 5.4 1.6 7.7 ~ 83.3
111 6.1 1.6 6.8 83.3
1525 S.3 1.6 8.5 83.3
2497 5.0 1.7 8.2 83.3
2771 5.3 2.2 7.2 83.3
4KER 5.1 1.8 7.6 83.3
2776 S.3 2.1 7.3 83.3
3538 5.2 1.9 7.6 83.3
4130 4.8 Z.1 8.0 83.3
53 5.6 1.S 7.S 83.3
650 S.1 2.2 8.0 $3.3
2163 5.2 1.8 8.4 83:3
1854 6.2 1.8 7.3 83.3
2384 5,5 1.7 8.0 83.3
2166 5.2 1.8 8.3 83:3
2498 5.0 1.8 8.1 83.3
3002 5.5 1.9 7.3 83.3
2778 5.2 2.0 7.5 83.3
a~°'~';": !°~'._ S_:.~.:.
oS~rw . 6 m t ~, ~~ W.~L
WO 91/11906 PCT/US91/00917
20~1~~-s2-
TABLc, 20 (Cont:.nued)
SAMPLE % % % %
#
PALMITIC OLEIC LINOLEIC
STEARIC
r"rrwrrr.,rrrw~wwrrwrwrww~mww-.m~~rwrmwrw~~rww~rww..m
2190 5,1 1.7 8.5 83.3
1779 5.I 1.9 8.0 83.3
2643 5.1 1.7 7.9 83.3
537 4.9 1.6 8.6 83.3
583 5.0 1.8 8.6 83.3
2870 5.4 1.7 7.5 83.3
3650 3.2 1:8 7.7 83.3
2074 5.1 1.8 8.5 83.3
2125 5,4 1.9 7.9 83.3
129 6.2 1.7 7.5 83.3
2213 5.2 2.1 8.0 83.3
3512 S.S 1.8 7.5 83.3
3498 5.3 2.0 7.4 83.3
2711 5.6 1.4 7.6 83.3
2225 5.0 1.8 8.4 83.3
69S 5.6 1.7 8.1 83.3
3445 5.2 1.S 8.4 83.3
I13 5.8 1.7 7.0 83.3
908 5.2 1.5 8.8 83.3
693 5.2 1.7 8.5 83.3
654 5.2 1.7 7.7 83.3
104 d.1 1.7 7.0 83.3
34.26 5.2 1.7 7.9 83.2
1618 5.2 1.9 8.0 83.2
3643 5.2 2.5 7.3 83.2
124 5.7 1.8 7.8 83.2
4.3 2.1 7.8 83.2
3364 5.2 2.3 7.4 83.2
1863 6.1 1.8 7.5 83.2
535 4.7 1.7 9.0 83.2
443 5.1 2.3 7.7 83.2
1523 5.6 1.9 7.9 83.2
1872 6.1 1.7 7.6 83.2
3543 S.1 1.8 7.9 83.2
2409 5.0 1.8 8.5 83.2
2600 5.0 1.9 8.0 83.2
2155 5.0 1.? 8.7 83.2
2193 4.9 1.5 8.8 83.2
3029 4.7 1.4 8.8 83.2
4804 5.4 1.5 8,2 83.2
2673 5.0 1.7 8.2 83.2
3572 5.3 1.7 8.0 83.2
436 5.1 2.3 7.9 83.2
3858 5.0 2.0 7.9 83.2
3586 4,9 1.8 8.2 83.2
3894 5.0 1.8 8.2 83.2
123 5.6 1.8 7.9 83.2
3214 4.7 Z.4 8.0 83.2
SU3ST~TL'T~ SF':ccl
WO 91/11906 PGT/US91/00917
~:. ..~,...~ .r_~y.t~,..
;..c;..~::w. ~ 1.:..~,,~~~. ,
-83=
TABLE 20 (Coati.~.ued)
SAMPLE # % % % 96
pALMTTIC OLEIC LINOLEIC
STEARIC
wwrrrrrrrrrrrrrrrwrrrwrrrrrrwr.rurrrw..wrrrwrwrrrrrnowwrrrr.nwmrwrwrw
3454
3.2 1.9 6.6 84.4
3828 5.0 1.8 7,0 84.3
3573 5.1 1.5 7.2 84.3
2830 4.6 1.6 7.5 84.3
2829 4.7 1.7 7.3 84.3
1579 5.2 2.0 7.3 84.3
2626 4.6 2.2 7.1 84.3
2391 4.4 1.5 8.3 84.3
4472 5.3 1.7 6.9 84.3
3592 5.0 1.6 7.4 84.2
3498 5.3 1.9 6.5 84.2
3601 4.7 1.7 7.6 84.2
1251 5.0 1.7 7.S 84.2
Z68? 5.0 1.7 7.1 84.2
1$59 5.1 2.1 7.4 84.2
2809 5.3 1.8 6.8 84.2
2147 5.0 1.7 ?.? 84.2
2775 5.3 1.9 6.7 84.2
1 4.2 2.3 7.0 84.1
SZ2 4.6 1.9 7.9 84.1
2510 5.1 1.7 7.1 84.1
3417 6.3 1.6 7.2 84.1
2s31 4.8 i.s 7.s s4.1
3130 ~ 5.3 1.9 6.8 84.1
3869 5:2 1.8 7.2 84.1
4212 5.3 2.1 6.6 84.1
2871 5.3 1.6 7.2 84.1
521 5.1 1.8 7.7 84.1
1247 4.9 1.9 7.5 84.0
2151 4.9 1.8 8.0 84.0
3455 5.4 1.9 6.7 ' 84.0
1253 4.8 2.0 7.6 84.0
484 4.S 1.5 8.3 84.0
3906 5.3 1.6 7.2 84.0
883 4.5 2.1 8.1 84.0
2317 5.0 1.6 8.1 84.0
3502 5.1 2.0 7.1 84,0
247 5.0 1.2 7.6 $4.0
4765 5.8 1.9 6.6 84.0
3649 5.1 1.7 7.4 84.0
2707 5.0 1.5 7.6 84.0
4599 5.1 1.9 7.2 83.9
3102 S.7 1.8 6.4 83.9
3101 5.4 2.1 6.4 83.9
1249 S.0 2.1 7.4 83.9
3506 5.1 I.7 7.5 83.9
534 5.0 1.5 8.4 83.9
2148 5.0 1.8 7.9 83.9
~~~~~-.T:Ti.: i r. ~~~e c
WO 91/11906 PCT/US91/00917
~~J~~4a~~
.:r..... ... _84_
Example I1
1987 Breeding Program and Results
The previously obtained lines were selfed in 1987 and
the seed analyzed for linoleic acid production. A total of
294 lines were selected after the 1987 season which produced
seeds having a linoleic acid content greater than 80%. The
range in linoleic acid content of these lines was 86.0% to
89.1%. The oleic acid content ranged from 3.9% to 8.0% and
the saturated fatty acid content ranged from 4.7% to 7.2%,
with one line at 7.6%.
The ratio of saturated fatty acids to linoleic acid ranged
from 0.054 to 0.084. The mean temperatures for July and
August were 71.3'F and 66.0'F, respectively. The results are
shown in Table 21.
WO 91111906 PCT/US91/00917
c:i;°:f
-85- , ., a ..~. ~ s i:~':";.
:: :~ '= w' 205. .43~
. TABLE 21
FIELD % % % %
- E~Y.__-__-__---_-- PALMITICSTEARIC OLEIC LINOLEIC
-._- # __-______________ 16:0 - -_ -__-
_-_ _-__- 18:2
18:0 18:1
7~22g-s _-_ 5.2 _____________ i:7 _____ ____ _____
________ 5.6 8a
______ a
7b4239-3 s.8 1.4 s.6 .
87
3
7b4226-1 4.8 l.s S.5 .
87
3
7b4239-4 4.4 1.8 s.3 .
87
3
7b3694-2 3.4 1.5 6.s .
87
3
7bs0-2 4.4 ' 1.5 6 .
8 87
3
7b4223-1 4.8 1.5 . .
s.l 87
3
7b4239-5 4.s 1.4 s.9 .
87
2
7b4238-1 s.2 1.6 6.1 .
87
2
7b4349- 4.3 1.3 6.1 .
I 87
2
7b4233-4 4.4 1.6 S.s .
87
2
7b117s-2 3.7 2.0 5.3 .
87
2
7b433o-s s.l l.s s.9 .
s7
2
7b369s-1 4.3 1.8 4.9 .
87
2
7b4233-4 4.7 1.6 5.4 ,
87
2
7b4133-s 4.6 1.8 6 .
5 87
1
7b4231-2 5.0 1.8 . .
6 87
1 1
7b4226-2 s.2 1.3 . .
5.8 87
I
7b 1274-1 s.0 1.7 5.2 .
87
1
7b3648-2 4.7 1.4 4 .
9 87
1
764328-2 4.5 1.5 . .
5 87
7 1
7b4108-2 3.7 1.5 . .
6.8 87
1
7b4239-1 s. l 1.3 5.7 .
87
0
7b3666-5 4.3 1.4 ~ 7.0 .
87
0
7b3643a-4 s.0 2.0 6.0 .
87
0
7b4108-2 4.s 1.4 2 .
7 87
0
7b4239-4 4.9 1.4 . .
5.4 87
0
7b4236-3 s.3 l.s s.4 .
87
0
7b4224-I s.2 l.s 6.4 .
86.9
WO 91/11906 . PGTfUS91/00917
f., ~,
~:. iw .. -86-
.,. '., '~ ~~a~,l,~c~Jr
TABLE 21 (Continued)
FIELD % % % % .
ENTRY PALMITIC STEARIC OLEIC LINOLEIC
______ ________
i 6:0 ___________ i 8:0 ____i _____ ___i 8:2 '_
-_______ 8:1______
7b4108-2 4.1 1.5 7.0 86
5
7b4236-3 5.6 1.3 5.7 .
86.5
7b4231-3 4.8 1.8 6.0 86
5
7b1274-3 5.0 1.8 5.6 .
86.5
7b4108-2 4.6 1.6 7.3 86
5
7b3684-1 4.5 1.8 6.0 .
86.5
7b4225-4 5.0 1.6 6.1 86
5
7b3684-1 4.3 1.7 5.9 .
86
5
7b4238-1 5.0 1.8 6.7 .
86
5
7b4249-4 5.0 1.7 5.9 .
86
5
7b4226-1 4.9 1.6 5.9 .
86.5
7b1274-1 5.3 1.6 5.7 86.5
~
7b4349-2 4.3 1.8 6.1 86.5
~
7b4236-2 5.2 1.5 5.7 86.5
7b2145-2 4.8 1.6 5.4 86
5
7b4237-2 4.9 1:5 5.7 .
86
5
7b2145-1 5.4 1.4 5.2 .
86.5
?b4359-2 5.1 1.8 6.3 86.5
7b1207-1 3.9 1.6 6.6 86.4
7b4108-2 4.3 1.5 6.8 86.4
7b4299-1 4.8 1.3 7.3 86.4
?b4349-1 4.5 1.6 5.5 86.4
7b4239-4 4.9 1.4 6.0 86:4
7b4359-5 4.8 1.7 6.1 86.4
7b4236-2 5.4 1.7 6.5 86.4
7.b1274-5 5.1 2.0 5.7 86.4
7b4132=5 4.5 1.5 6.7 86.4
7b3668-2 4.8 1.6 5.5 86.4
7b4238-1 5.2 1.7 5.8 86.4
WO 91/11906 PCT/L1S91/00917
~:;:,
_87_
TABLE 21 (Continued)
~205143~
.:..t ;.w~, ..,
FIELD % % % %
ENTRY PALMITIC STEARIC OLEIC LINOLEIC
__________________
i 6:0-__-_______ i 8:0 ___-i ____ __-i
________8:1-______8:2 '_
7b3648-2 ~.0 2.0 6.0 86
0
?b2115-1 4.2 1.6 6.6 .
86
0
7b4228-4 5.1 1.9 6.0 .
86
0
7b3658-1 5.0 2.0 7.0 .
86
0
.
7b4229-5 4.6 1.6 6.4 .
86
0
7b3665-5 5.0 2.0 7.0 .
86
0
7b4236-3 5.6 1.4 7.1 .
86
0
7b3667-1 4.4 2.0 7.0 .
86
0
7b43~9-4 5.2 1.5 6.1 .
86
0
7b4238-5 4.9 1.7 6.4 .
86
0
7b4349-4 4.3 1.8 6.3 .
86
0
7b1274-1 5.1 1.8 6.2 .
86
0
7b4330- I 5.2 1.5 6.7 .
86
0
7b4168-5 4.6 1.4 7.1 .
86
0
7 b4307-2 4, 8 1.? 7. I .
86
0
7b4189-1 4.6 1.6 6.8 .
8b
0
7b4244-2 4.7 1.6 ' 6.5 .
86
0
7b4224-2 5.6 1.1 6.4 .
86
0
7b3626-5 3.1 1.6 8.0 .
86
0
7b2171-1 4.6 1.4 6.4 .
86
0
7b366G-4 4.4 2.0 7.0 .
86
0
7b3631-4 5.2 2.0 5.2 .
86
0
7b3685-4 4.5 1.6 6.6 .
86
0
7b 1272-4 5.1 I .8 4.7 ,
86
0
7b3635-1 5.0 2.0 6.3 .
86
0
7b4168-5 4.6 1.7 6.6 .
86
0
7b3644-2 5.1 2.0 6.0 .
86
0
7b4224-4 4.8 1.7 6.7 .
86
0
7b366(r3 S.0 1.4 7.0 .
86.0
WO 91/1 1906 PCT/US91/00917
...~sr,:.~, .,:m~~~t,<~ -~8-
l:.vZ
~i~J~~,L~,''~
Example I2
1988 Breeding Program and Results
These 294 lines were selfed in 1988 and the seed
analyzed for linoleic acid production. A total of 300 lines
were selected after the 1988 season which produced seeds
having a linoleic acid content greater than 80%. The range
in linoleic acid content of these lines was 83.0% to 90.4%.
The oleic acid content ranged from 3.3% to 12.4% and the
saturated fatty acid content ranged from .5.1% to 8.3%. The
ratio of saturated fatty acids to oleic acid ranged from
0.056 to 0.099., The mean temperatures for July and August
were 73.7'F and about 70.6'F, respectively. The results are
shown in Table 22.
WO 91/11906 PGT/US91/00917
:,:.'_,'-'-
:,
-89-
TABL~ 22
,~~:~(~5~:43~
FIElD
?i
a~u sic a.r~cuNO~e~.o
ma
.
te:o tea ,a$
.- -_
__._ t s:o _.
-._.
SEbt0E31mS.a 1.B 7.1 8d.3
a8btO80'tn,6.6 !.6 7.0 X4.3
fibt732-tm'S.t 2.1 65 E't,J
Bt1b173615.3 !.0 G.7 a4.3
dtlbt314-Im0.3 t.i 7.~ 64.3
6aat713tmS.5 t.a 8.0 80.~
88~b2d7325.0 t 8 7.9 84.3
88b2765-4m3.~ t.7 d.0 8.3
aeb2ao9-15 ~ ~ ~ ' "' s
m ~
aeb2a33 s.1 t .9 a.7 ea.3
t
&7D325E16.~ 2.0 7.6 64.3
~ib3647-35.! t.t b.7 bt.~
MeE3b9~tm5.7 2.0 0.5
MB83t399tm6.a '.9 6.6 &4.2 .
sa01201-ta.7 1.2 1.7 X4.9
rn
88bt202tm4.7 2.0 T.7 D1.!
$801066-14.9 t.6 i.3 34.2
88b3b83tm.S.t ~.7 7.J N.2
AAb27iCat,t5.1 1.9 7.7 a4.t
8HbE8252m5.2 t.6 7.8 84.2
aaD~9sata 9 z.o s.' as.s
MH93896tm5.3 t.9 7D Wt
fi81l.3709ltrt5.6 2.0 0.8 :4.1
iib10e9tm5.2 t.6 7.5 b~.t
a8b12t4tm5.D 2.0 7 d4.t
0
aeD1811.Im4.9 2.0 9.0 :1.1
EtltfZ664.6S.4 i.8 Z.i 84.t
~dZ7742rnS.5 t.7 9.9 2a.t
SaD277SIret5.6 2.0 7.5 :4.t
eaozaoo-tms.t 2.0 T.i a.t
eeb2e27t~n~.s t.9 Ts as t
6Hb1175lrr.5.5 :.6 7. li4.0
~
moas~aam5.a t.e '.a a4,o
38bat73t.3m4.3 2.D a.E 3a.0
dd02:b51m5.i Z.1 3.7 ia.0
38b~itatmS.a t.i T.2 u.0
a90387Sd4.8 t.9 7.5 aL0
.18839,~tmS.e 1.9 8.0 d3.9
Mbe3700-tm5.8 2.0 0.3 e1.9
88Cat5tm5.3 ! 6 7.' a3.!
sQe,ec>ia.b t.s T.T o0
tA
880t097.tm4.6 t.p d.s a3.D
WO 91/11906 PCT/US91/0091'7
cf '~r,
.~.~; -90-
='~3L~ ~2 (Co.~.t..~.;:ec.)
FIELD
ENTRY ".~ % !~ 6
?ALMITIC9TEAlItCOI.BC't~NOIEIC~
16:0 18:0 'e_t __ '~Z _ .
,_
aab~a2.z~s.a t.s 9.4 aa.4
aebzez3.ta.a z.o 3.3 ea 4
eeo2msm 5., :.y 7a si.s
atlbQ7682mS.E 2.4 ?.1 5J.4
edoa7al3md.4 tt T.2 53.4
88b>:7832m5.4 2.t 9.6 33.4
28b2765Sm5.3 2.3 a.2 33.4
aabi7se3a.~ ~ 9.: e~.~
0
enbzdoa-Zta!.l 2.2 7.4
corex,~ sa :.a ~.7 ss.4
stlboo33-a~.B 2.0 d 63.4
7
dd.340b15.7 2.0 7.8 83.4
aab768t15.d 1.9 7.I A3.4
MB63897tm'5.7 :.v 73 D3.J .
M88-3it)G.im5.J 7.2 7.2 !3.3
SE010811:.8 t 7. d3.J
9 t
3Db10901m6.8 . a.5 E.1.3
s
886t162tm5.4 t,a 3.3 53.3
d801818tm4 4 2. d.? 33.3
t
d8~t972t5.6 !.9 7 a3.i
3
8Ab1943tm:.4 2.1 ..5 33.3
dA0t7781m4.9 t.a ~.t e3.3
pB~rZ.efiA-Jm5.1 ? '.5 aJ.7
2
bGbp49~-2m3.1 t 7.7 83.3
3
a8tkde18tm5.7 t.1 '.3 :3.3
Edb=btdZm5.5 2.1 T.3 a3.J
88b26r,3tms,? :.3 3.1 33.3
88b285S-tm5.: 1,a 7,? 33.3
d8bs701t:.3 t.5 ?.S 83.3
dE4~7025mo,t 2.9 7.9 !.3.3
886a7054m4,p 2.3 8.3 !3.!
88~76~a~5.2 ~.; a,g
d~l~im 5.5 2.t a.1 a3.3
80b2834tm~.0 1.0 8.3 83.3
tabZB4C-t5.3 1.0 9.1 A3.3
ems:escna a :.1 a.o e3.a
aaext~.ta.t to a.a a3.2
aebsz9t d.a t.s 6.a a3.=
1A099Stmd.T ..a 8.d 63.2
E8b103itm4.9 ~ 5.a a3.3
8
88b1CA~1m5.0 :.7 7.9 D.1.2
aeat815tm~ 4 Z.1 a.4 S3.2
WO 91/11906 PGT/US91/0t1917
.,::;:
~n'~ .,::
- ~ ; ' .~'~.~ K~tw
~O~J1.~~~~
TABLE 22 (Continued)
FIELD
ENTRY
pALMITIC S'Y'EARICOLEIC LINOLEIC
rw-w-wwwrw ww-ww-w------w,.wwww--wwwwwwwwwww
# I6:0 I8:0 18:1 18:2
wwrwrw w..ww.www-w---w'wwww www- w-r.-ww..
$8b1102-lm 5.4 1.8 6.7 83.9
88b1104-lm S.1 1.9 7.2 83.9
88b1548-lai5.1 1.9 7.2 83.9
88b1751-lm 5.4 2.1 7.9 83.9
88b195S-1 5.3 1.5 7.5 83.9
88b2142-lm 5.0 1.8 7.3 83.9
88b1728-im 5.5 Z.5 7.5 83.9
88b2b93-3m 5.3 1.9 7.2 83.9
8862770-3m 5.2 1.9 7.3 83.9
8$bZ813-1 5.4 2.0 ?.1 83.9
88b364S-1 5.9 1.5 8.4 83.9
8$b241-1 S.I 1.5 8.2 83.8
88b1093-im 5.1 1.7 7.7 83.8
88b1333-im 4.8 ~ 2.1 8.3 83.8
88b1734-lm 5.2 1.8 ~'.3 83.8
88bI75Crlm 5.5 1.9 7.9 83.8
88bZ343-1 5.5 1.9 7.8 83.8
SSb2628-4m 5.3 Z.0 7.3 83.8
-
88b2628-Sm 5.2 1.8 7.8 83.8
88b27I4-4 5.4 1.8 11.5 $3.8
$8b2765-3m 5.5 2.2 7.7 83.8
88b2771-2 5.6 1.7 8.5 83.8
8sbz776-lm s.l 2.0 8.z s3.s
SSb2814..Zm5.3 1.9 7.6 83.8
88b2817-2m 5.6 1.8 7.7 83.8
88b2818-lm 5.2 2.1 7.8 83.8
88b2851-1 5.5 1.9 8.3 83.8
88b329I-1 4.8 1.8 8.6 83.8
88b2143-lm 5.2 1.6 7.3 83.7
88b2683-4m 4.8 1.9 8.7 .83.?
88b2774-lm 5.8 1.7 7.9 83.7
88b3644-1 S.0 1.7 8.2 83.7
88b3673-1 5.5 1.8 8.4 83.7
Nig8-3700-lm5.6 2.0 7.1 83.6
88b804-lm 5.2 1.6 7.9 83.6
88b1092-im 4.9 1.7 7.8 83.6
88b1096-lm 4.8 1.5 9.2 83.6
88b1817-im 4.8 Z.1 8.3 83.6
88b1981-1 4.1 2.0 9.0 83.6
88bZ345-1 5.3 1.7 9.1 83.6
88b2518-4m 5.4 1.8 7.1 83.6
88b2598-3m 5.3 1.7 $.0 83.6
SIJB~TeT~.a ; ~ SS~~~'~
WO 91/11906 PGT/US91/00917
rf :<:
-92-
=:~HLL 22 (Cart=::ue~)
FIELD
ENTRY %
p~M~C STEARIC OLEIC LI1VOLEIC
Y
rnwwwrrrwwwwrW --www
..wr-wYwwYw
wYww-w wwwuwww w--wYYwws
16:0 I8:0 18:1 18:2
1w..11w1w--lNlwrwllYw-rrl..lYw ----w..-YNIw-www-1Y--1wwwwwNIN11
88b2349-1
7
1.9 7.5 83.2
88b2481-2m 5.3 1
9
. 7.5 83.2
88b2481-4m S.3 2
0
. 7.5 83.2
88b2492-3m 5.0 2 4
S 7
. . 83.2
88b2506-2m S.4 1.9 7 2
5 83
88b2588-1 5.5 1.9 . .
9 83
0 2
88b2S$8-4 5.5 1.S . .
9 83
9 2
88b2618-1 S.b 1.8 . .
4 2
9 83
88b2692-lm 5.g , .
2
$8b2711-3m S.0 2.2 7.6 83.2
$8b2713-Zm 5.1 2.2 7.9 83
2
88b2763-Sm 5.2 2.1 7.7 .
83
2
88b2768-1 S.5 1.9 .8.7 .
83
2
88b2$56-1 5.2 .
1.8 7.9 2
83
88b30S2~1 5.2 2.0 8 .
9 83
Z
88b3668-1 5.3 Z.0 . .
7.6 83
2
1V1$8-3692-lnn5.4 1.9 7.7 .
83
1
88b238-1 5.4 L9 7.7 .
~ 83
1
88b809-lm S.5 2.2 7.6 .
$3
1
88b1084-lm 6.1 1.6 7.6 .
83
1
88b1461-lm 5.2 2.1 8.8 .
83
1
88b1727-lm 5.2 2.4 7.8 .
83
1
88b1729-lm s.z 2.7 7.9 .
1
83
88bi783-lm 4.9 Z.0 8.3 .
83
1
88b1101-lm 5.2 1.8 8.0 .
83
1
88b1735-1 5.6 2.0 7.4 .
1
83
88b2354-Im 5.6 2.5 7.5 .
1
83
8862630-4. 5.5 2.4 9.0 ' .
83
1
88b2654-2m 5.3 2.5 7.7 .
83
1
$8b2693-lm 5.1 2.0 7.8 .
83.1
88b2763-3m 5.3 Z.1 8.5 B3.1
88b2767-3m 2.9 2.5 10.4 .
$3.1
88b3464-1 5.3 1.8 8.8 83.1
88b3517-1 5.4 1.7 9.8 83.1
88b3674-1 5.6 L8 7.9 83.1
$8bI054-lm 4.5 2.1 9.1 83.0
88b2713-5m 5.1 2.2 8.2 83.0
88bI067-lm 5.2 2.0 8.4 83.4
88b1174-lm 5.4 1.7, 7.8 83.0
88b1176-lm 5.5 1.6 8.0 83.0
88b1977-1 4.7 1.8 4.3 83.0
88b1978-2 4.8 1.6 9.4 83.0
SU'~v''';'"~.' :~ ~~:~~'1
WO 91/11906 PGT/US91/00917
fy:
-93- ' 2oSJ ,
:. j
Example 13
Breeding Pedigrees of
Representative Safflower Varieties
The following high linoleic acid safflower varieties
were obtained by the described breeding pedigree.
(1) 89B7503-5
female uarent: Roundup Tolerant Selection 1961 world bulk
composite crossed with
male parent: [87-42-3/AC-1//(87-42-3/AC-1//AZ2698/012-
251-3-6]///(87-42-3/AC-1//88-74-2/N-10)//(Mex.dwarf-
2//OL35-2/PCM-1)//white 80 nuts. pigmentless
(2) 89B7613
female barent: 87-42-3/AC-1//Cargill dwarf crossed with
male parent: 87-42-3/AC-1//(87-42-3/AC-1//AZ2698/012-251-3-6)
(3) 89B7311-1
female parent: 87-42-3/AC-1///[[(87-42-3/AC-
1//AZ2698/012-251-3-6)]//87-42-3/AC-1] crossed with
male parent: [87-42-3/AC-1//(87-42-3/AC-1//AZ2698/012-
251-3-6)]///[(87-42-3/AC-1//88-74-2/N-10)//(Mex.dwarf-
2//OL35-2/PCM-1)//white 80 nuts. pigmentless
(4) 89B7557-3
female parent: [(Biggs/PI195895//C.L.1653/87-42-
3)//UCD1982-620/Oker] crossed with
male parent: [87-42-3/AC-1//(87-42-3/AC-1//AZ2698/012-
251-3-6)]///[(87-42-3/AC-1//88-74-2/N-10)//(Mex.dwarf-
' 2//OL35-2/PCM-1)//white 80 nuts. pigmentless
(5) 89B7475
female parent: 87-42-3/AC-1//Cargill dwarf crossed with
male Qarent: [87-42-3/AC-1//(87-42-3/AC-1//AZ2698/012-
251-3-6)]///[(87-42-3/AC-1//88-74-2/N-10)//(Mex.dwarf-
2//OL35-2/PCM-1)//white 80 nuts. pigmentless
WO 91/11906 PCT/US91/00917
..-s~..~ .r..~:-~-.;N':
..
-94-
(6) 89B934-3
female parent: 88-45-4/S-208//N-4051/Gila crossed with
male,p~ent: 87-42-3/AC-1
(7j 89B7448-1
=emale .parent: (87-42-3/AC-1///[[(87-42-3/AC-
1//AZ2698/012-251-3-6)]//[(87-14-B/N-10)//87-42-3/AC-
1)//(87-42-3/AC-1)//AZ2698/012-251-3-6]]//white 80 nurs.
pigmentless crossed with
male arent:
87-42-3/AC-1//[(87-42-3?AC-1)//AZ2698/012-251-3-6j].
The oil content of 89B7448-1 is 4.4% palmitic acid, 2.0%
stearic acid, 5.9% oleic acid and 86.0% linoleic acid. The
characteristics of this line are similar to other commercial
high linoleic acid lines.
Additional test results obtained on the subject matter
of the invention.
WO 91/11906 PGT/US91/00917
r~',...:;
~~51435
~ .,
OLEIC 1990 IV'CItEASE OIL QUALI'T'Y AVALYSES
OPEN TOTAL
FIELD LAB SEED OPEV % % %a %
ErITItY SA1PLE OIL SEED PALVIITICSTEARIC OLEIC LINOLEiC
.............__.__._...__.......__%
_.
_
._........_........._..................r......._.._.....__.__..._..._..........
..........
.w..w......._...._.. NVIR grams 16:0 18:0 I8:1 I8:?
.....wr
w.._._~.._.....o...o._._._~.~._.___..._..._.....__.__..___r..._..........._..._
....__.._........r
90-OL-81111142 3.3 1.3 83 10
7 0
90-OL-80911140 3.4 1.1 . .
83 10
6 7
90-OL-8041i 13S 3.4 1.2 . .
83.2 10.5
AvG 83.5
WO 91/11906 PGT/US91/00917
..;,..... : .:
.,..;-,. :.
.. ~ ~.; ~ ..
, _;:_
...
_96_
a
- 1989 Analvscs
89b-
Oil
Quality
TOP
rII:LD LAB OPEN OLETC
LI1ES
TOTAL
B'~'TRY SAMPLE OPEN PAL~iITIC
filrCD ''
c OLEIC "'o
YYYw OXI' SEED " C 9
~ STEAI2I
Y
o LINOLEIC
Y --~y.n.,o-rm,naybss-.Y..
w
1.1-
-.o~.--~--n..pf.-oo...w.......-iws--o-w.-..--.o.
.wYYY.w.. --W l~iblR trams 1G:0 18:0 18:1 15:2
w.Y w.w....
89B-6394 . r...w.wYY-..~-.-rww...-...... __..
I __..
_ _
- 1600 S 1.1 106 3 8 ..$w
3 . _.-.
8913-6739-11927 36.8 19 . I.3 j.5 4
3
4
. 1.6 85.5 8.0
89B-6I27-11366 43.4 76 3
6
. 1,S g5,4 7_g
89B-6397- 1603 50.4 99 4
I 3
. 1.2 85.4 8.6
89B-6709-11898 49 42
1
. 3.2 1.1 S5.4 8.8
89B-6732-11920 40 S
3 3
. - 3.6 1.4 85.4 8.2
89B-6704-11893 51 37
2
. 3.3 1.3 85.3 8.4
89B-6709-42421 49 42
1
. 3.I 1.4 85.2 8.6
8913-6726-52426 45 9S
1
. 3.6 1.1 85.0 8.8
89B-6731-11919 51 G2
1
. 3.6 1.1 85.0 8.6
8913-6731'-~2429 51 62
I
. 3.4 1.1 85.0 8.9
89B_6563-11768 5L2 290
3.3 0.9 84.9 9.3
$9B-6024-11288 53 181
8
. 3.3 0.9 84.8 9.4
89B-6294-11508 49 75
9
. 3.6 1.1 84.8 9.0
898-6726-I1914 45 95
1
. 3.7 1.2 84.8 8.7
89B-6739-32435 36 I9
8
, 3.4 1.7 84.8 8.4
89B-6294-51668 49.9 7S~ 3
5
.. 1.2 84.7 8.9
89B-6731-32428 51,1 62 3
6
, 1.2 84.7 9.0
89B-6752-42439 51 37
4
. 3.6 1.4 84.6 8.7
89B-6563-S2150 51.2 290 3
6
. 1.4 84.5 8.3
89B-67011 1891 h~/A 14
3
6
, L4 84.5 8,9
89B-6752-11939 S I 37 3
.4 6
. 1.2 84.5 8.7
89B-6485-11693 54.0 89 3
6
. 1.2 84.4 9.3
8913-6744-11930 ,50,6 43 3
5
. 1.1 84.4 9.2
89F3-6746-11933 49.0 18 3
S
. 1.4 84,4 8.6
8913-6752.22437 5I,4 37 3
7
. 1.1 84.4 9.3
89B-6018-11273 52 10G 5
2 3
. .. 1.0 84.3 9,6
89B-6709-32420 49.1 42 3
6
. 1.3 84.3 9.3
89B-6709-~2422 49.1 42 3 1 84
6 4 3
. . , g,0
89B-6732-52433 40.3 35 3.8 L7 84 8
3 7
89B-6736-I1924 N/A 12 3.7 1.6 . .
34 8
3 8
89B-6765-11950 ~9.4 23 3.S I.2 . .
84 9
3 1
89B-6159-11395 41.6 118 3.7 2.4 . .
s4 8
z 8
89B-6483-11691 44.6 86 3.6 1.2 . .
84 9
2 3
89B-6704-42417 51.2 37 3.5 1.3 . .
84 4
2 9
89B-6732-22430 40.3 35 3.3 1.3 . .
84 g
2 7
89T3-6022-11283 52.3 S6 3.3 1.3 . ,
84 10
1 0
8~B-G70G-11895 ,51.0 60 3.4 I.2 . .
84 g
1 3
89B-6720-22423 45.1 9j 3.6 1.1 . ,
84 9
1 6
89B-6766-11951 50.6 26 3.7 1.3 . .
S4 9
I 0
89B-6145-I1382 50.2 367 3:8 1.~ . .
84 9
0 3
89B-G59~-1179.5 49.3 63 3.G 1.5 . .
R4 9
0 4
89B-6669-I1860 N/A 17 3.5 1. . .
54 9
0 7
8gB-6710-1Ig99 47.4 42 3.6 1.6 . ,
g4 3
0 9
S9I3-6713-11902 49.4 .i0 3.4 1.3 . .
84 9
0 6
8913-6732-32431 40.3 3S 3.6 1.7 . .
g4 9
0 2
8913-6741-11932 51.1 :~9 3.6 I .2 . .
34.0 9.4
WO 91/11906 PCT/US91/00917
(~';.
I989 Analyses2osl~~'.r'~'v
89b-
Oil
Quality
TOP OLEIC
MELD LA$ OPEN LINES
TOTAL
Ei~?RY S~~MPz,~ OPEN PALMITIC %
SEED
OLEIC%
OTX. SEED % STEARIC %
%
LINOLEIC
Iwwwwr._r_r_Nw_wrw.r_.-.-.w.y.........._.__o-.m.rr...-
_......_.._..o_...__.w..-..._....uww-...o......
#
....
-- - _ # hylR grams 1G~0 18:0 18:1 18:2
....--r
-- _-_._-__..r..___...._.-_ __.w.-
89B-6027-I1302 --._._. _.-.. ..... .. ._.
"._ _..... -. -._.
89B-6127-51664 SS.O 79 3.4 i.0 83.9 10.2
43 76
4
. 3.9 1.2 83.9 9.2
898-6731-22427 51 62 3
1 7
. . 1.3 83.9 g.4
89B-6747-11934 N/A OI 3
9
. 1.6 83.9 9.1
89B-6359-11572 53.3 39 3
5
. 1.2 83,7 9.9
898-6705-11894 50.9 76 3
S
. 1.2 83.7 10.1
8~B-6735-11923 N/A OS 3
7
. 1.5 83.7 9.1
89I3-6478-11687 44 84 3
4 7
. . 1.1 83.6 I0.1
89B-6711-11900 46.I 19 3 L3
5
. 83.6 10.1
89B-6728-1. 44.7 96 3 1
1916 8 3
. . 83.6 9.8
8913-6762-11947 51.1 80 3 L2
3
. 83.6 9.7
898-7429-12355 52.2 51 3 1
9 5
. . 83.6 9.4
89B-6146-11383 49.7 349 3 1 83
9 4 5
. . . 10.0
89B-6294-41667 49.9 75 3 1 83 9
6 3 5 8
89B-6393 1599 52.0 I33 . . . .
1 3.5 1 83 I0
1 5 5
89B-GG16-11888 53.5 2s 3.8 . . .
4 83 9
1 5 8
89B-6701-S2414 N/A 14 3.5 . . .
1 83 I0
3 5 2
89B-6730-11918 50.3 27 3.5 . . .
1 83 9
1 5 9
89B67392 2434 36.8 19 3.5 . . .
1 83 I0
6 5 0
89B-6068-I1337 47.4 119 3.6 . . .
1 83 10
1 4 5
89860981 1518 52.4 55 3,6 . . .
1 4 9
2 83 9
8911-6391-11598 50.3 78 3.5 . . .
I 4 10
2 83 4
89B-6438-11641 55.9 5I 3.9 . . .
4 83 9
1 4 8
8913-6704-22415 51.2 37 3.5 . . .
1 R3 1
3 4 10
8913-7427-12353 SG.1 64 3.7 . . .
1 4 10
3 83 3
89B-6026-11297 SI.6 108 3.4 . . .
1 83 10
2 3 5
89B-6127-41 43.4 76 3.8 . . .
G63 1 83 10
2 3 0
89B-6142-I1380 50.1 401 3,9 . . .
1 83 9
4 3 9
89B-6621-I1819 52.3 107 3.6 . . :
I 83 10
5 3- 0
89B-6725-I1913 50.0 13z 3.4 . . .
1 83 lo
6 3 l
89B-7037-I1999 34.1 44 3.5 . . .
83 11
3 3
R9B-6136-I1375 51.0 3g7 3.9 1 . .
5 83 2
2 10
R9B-6785-11966 N/A 5 4.3 . . .
1.6 83.2 9
0
89B-7439-I2365 42.4 104 3.6 1 83 .
6 2 9
8
R9B-6127-216GI 43.a 76 3.6 . . .
1 83 4
3 I 10
8913-6129-11368 53.3 S8 3.8 . . .
1.2 83.1 10
I
89B-6358-I1571 51.8 85 3.4 1 83 .
1 1 10
6
898-6482-11690 46.9 I35 3.5 . . .
1.0 83 10
1 8
89B-6671-11861 49.2 25 3.8 2.0 . .
83 9
1 7
S9B-6691-11878 50.7 35 3.5 1.3 . .
83.1 10
5
H9B-6708-11397 49.9 87 3.6 1.2 83 .
1 10
1
8913-ti763-11948 48.6 20 3.6 1.0 . .
83.1 10.2
89B-67GR-11953 50.1 G9 3.7 1.0 83.1 10.3
$9B-7432-1235$ 45.6 73 3.9 1.4 83.1 9
9
R9I3-6028-I1304 53.9 135 3.4 1.2 83.0 .
11.3
S9B-6029-11309 52.3 97 3.4 1.3 R3.0 IO
S
;i9B.640I-11606 X8.1 26 4.0 1.5 83.0 .
9.R
WO 91 / 11906 - PGT/US91 /00917
~~~~.W .)
_98_
1990 :~~iontola-2000 Safflower Singie Row Results
OpEV TOTAL
T'IELD LAB
SLED OPEN % % g'o a'o
ENxI~'~' SA1~IPLEOIL SZ:ED
%
PAL~I ITIC STEARICOLIrICLINOL)~IC
r..____w___..,r_.___._.._____..._______.._..r_.
.._ _._.._._._......_...._.r_._._.___.._..______.._._.._
... ..___"..-.____.___-
....~_._.__........._.__._._....._N~IR crams zG:O 18:0 . 18:1 z&:z
_
.__________........______________...______..____._______.__._.________.___._.__
._.
6720-4 3264 3.8 L4 82 10
6700-S 8 0
3225 4.0 1.4 . .
63404 2954 82.3 9.5
3.9 1.4 82.1 9,9
63801 ~ 1647
3.8 1.6 8z.1 II.I
6380-1 3593
4.0 1.6 81.5 11.1
6720-5 3265
4.0 1.4 81.4 11.3
6340-3 2953
4.4 1.7 81.4 10.9
6720-3 3263
4.4 1.4 81.0 11.5
6720-1 2033
3.8 1.6 80.9 12:1
6340.1 1583
3.9 I.2 80.6 12.9
6700-I 2014
4.0 L7 80,5 12.0
63804 2966
4.1 L4 80,2 12.4
6380-3 2965
3.9 I.2 80,1 13.1
6720-2 3262
4.0 L1 80,0 13,G
6700-4 3224
4.1 I.s so,o 12.0
6900-1 2215
4.2 , 1,5 79,9 13.0
6740-1 2054
4.1 ~ I.3 79,8 12.9
6020-1 1259
. 4.0 1.4 79,8 13.2
6300-1 ~ 1542
4.0 1.3 79,6 13.2
6500-1 1811
4.1 1.6 79,5 13,1
6140.1 1381
6340-2 2952 4.0 1.4 79.3 13.G
G
2
. 1.7 79.3 l I.3
GSSO-1 1892
3.7 1.4 79.0 14.3
av~ 80.6
WO 91/11906 PGT/US91/00917
Py::i=''.
_99_
1998 Oleic S~fftower Single Row Results
OPL~N TOTAL
~LD LAB SEED OPEN % % % %
ENTRY SAM1'LE OII. % SEED rALMTTrC STEAItrCOLEIC LINOLETC
w-111bwrvw/~Ilwwww.,ww
w w -~wwlnlwwrW .-- ww wwwww-wN111N1-11-ww-wwww~
# ~ NMR, crams 16:0 18:0 18:1 18:2
-wwwww-w #
ww
3339 w..wwwwww rwwrwwwww.wwww-w-wwwwwwwwwr
6751-3 3.5 L2 85.? ?.3
6296-2 292? 3.0 1.2 85.5 8.2
6298-3 2936 3:2 1.2 85.3 7.9
6132-3 2815 3.3 1.0 85.2 8.5
6775-S 3393 3.3 1.2 85.I 8.4
6?49-3 3331 3.5 LS 85.I 7.8
6748-4 3328 3.2 1.3 85.1 7.4
6804-2 3459 3.4 1.3 84.9 8.5
6?51Z 3338 3.6 Ll 84.9 I0.4
6789-3 3427 3.4 1.2 84.8 8.9
6'152-4 3344 3.3 1.5 84.8 8.0
6697-4 3220 3.2 1.1 84.8 8.8
6095-4 2?59 3.3 i.2 84.? 8.S .
6?47-4. 3324 3.5 1.2 84.5 8.6
6379-2 2960 3.S 1.0 84.5 8.7
6585-3 3124 3.5 1.1 84.5 8.8
6218-3 28?I 3.? L2 84.5 9.0
649?-S 3049 3.4 L7 84.5 8.5
6681-2 3189 3.5 1.3 84.4 8.4
6298-S 2938 3.5 1.3 84.4 8.4
6298-2 2935 3.6 i.i 84.4 8.8
6208-3 2847 3.6 1.4 $4.4 8.4
6749-1 2063 3.4 1.2 84.4 9.2
6330-5 3150 3.4 L6 84.4 8.5
GOG8-2 2?09 3.5 1.0 84.4 8.G
6585-5 3I2G 3.4 1.3 84.4 9.4
6073-2 2717 3.5 0.9 84.3 9.3
6658-S 31?I 3.5 L3 84.3 9.1
6?61-5 3369 3.3 - 1.0 84.3 9.3
6632-3 3157 3.8 1.4 84.3 9.Z
6095-2 2757 3.5 i.i 84.3 9.1
G0G9-5 271 G 3.4 1.1 84.3 9.0
6693-2 3127 3.6 1.0 84.3 8.9
6218-2 2870 3.4 1.4 84.3 9.1
6631-2 3152 3.5 1.5 84.2 9.2
6525-4 3080 3.5 1.2 84.2. 9.3
6325-1 3589 3.9 1.4 84.2 9.1
6498-2 3050 3.5 1.3 84.2 9.4
6?59-2 3362 ~ 3.2 1.3 84.2 9.3
~
6?52-3 3343 3.3 1.5 84.2 9.I
6448-2 2981 3.4 1.2 ~ 84.1 9.0
6123-3 2795 3.G 1.3 84.1 9.1
6913-4 3523 3.8 1.4 84.0 8.9
~UBSTiTUTL SH~E'1
WO 91/11906 PCTlUS91/00917
-100-
r,~~S~
1990 Oleic Safflower Sinble Row Results
OPE1' TOTAL
FrIrLD LAB SEED OPZrN % %
'
F%i~lTf1 SAMPLEpIL SEND PALMITIC S'xEAI'LXCOLEIC LIYpLEIC
Y %
r.mrrrrrrrrorr
rr-~...-.wrwrr--r.,--rvrr r-r-.----r-..rr--..r.rr--.r-------.--m-
mwmuwrw-m..rrrrr-.-o---.--
~
-.- ~ NhtR grams ~6:o is:o Is:z Is:2
Yrr
rrrrrrrrr rrr-r-rrrr-rr---r~---r--rrrr-r-rr-rr rrrrrr -r--r-----r----r-
67575 3361
3.S 1.3 83.9 10.8
60785 2736
3.4 1.0 83.9 10.0
6747-1 ' 2061
3.6 1.1 83.9 9.4
6656-3 3161
3.3 1.3 83.9 9.5
6209-4 2852
3.3 1.1 83.9 9.3
6299-3 2940
3.5 I.2 83.9 9.~
6632-4 3158
3.4 1.3 83.9 8,
60964 2764
3,6 1.2 83.9 9.2
6252-I 3579
3.7 1.5 83.8 9.4
6301-1 3587
3.5 L7 83.8 8.2
6658-3 3169
3.7 1.5 83.8 9.0
6236 I 3574
3.4 I .0 83.8 9.8
6069-4 27IS
3,6 LZ 83.8 9.3
6235-4 2893 '
3.3 1.4 83.8 9,8
6130-4 2812
3.5 I.1 83.8 9.6
8' 1 I-1 1142
3,3 1.3 83.7 I0.0
6..673 2920
3.2 1.4 83.7 9.6
6097-4 2768
3.3 1.1 83.7
67663 3375
3.5 1, I 83.7 9..5
6249-2 2907
4.0 1.4 83.7 9.3
6301-3 2944
3. i 1.2 83.7 9.8
6379-5 2963
3.6 1.1 83.7 9.4
6749-2 3330
3.4 1,0 83.7 9.S
6703-3 ~ 3235
3.5 I.0 83.7 9.3
6;179-5 349=1
3,9 1.5 83.7 8.7
6585-2 3123
3.3 1.0 83.7 9,5
6883-3 3 506
4.0 1.9 83,7 8.2
6065-2 2701
3,6 1.0 83.7 ' 9.6
6790-3 3431
3.7 1.2 83.7 9.4
6497-2 30.6
3.7 1.4 83.7 9.4
8091-1 1140
3.4 1.1 83.6 10.7
606S-4 2703 3
4
. 0.9 83;6 I0.3
6753-4 3348
3.6 1.6 83.6 8.9
6753-2 3346
3.3 1.0 83.6 8.6
6068-3 2710
3.7 I.1 83.6 9,3
6750-4 3336
3.4 1,1 83.6 9,0
6221-4 2876
3
6
6499-1 36I~ . I.4 83.6 9.7
6244-5 2906 3'8 ~ 1.5 83.6 9.3
69 i S-2 3242 3.8 1.4 83.6 9.2
6545-1 1857 6
6789-2 3426 3.4 1.1 53.6 10.1
3
6
. 1.1 83.6 9.S
0073-3 2718
3
S
. 1.2 S3.6 9..:
60 7 G-4 2727 3
6
. 1.2 83.6 9.6
GSgS-'~ 3125
3
6
6299-I 3586 . 1.2 83.6 10.1
6748-S 3329 g
3.5 I.0 s3.~ 1
.4
WO 91/11906 PCT/US91/00917
;:n
-101- ' '
1990 Oleic Safflower Single Row Results
OrEN TOTAL
YIELD LA13 SEED '
QpEN 9 ~ r, cr
E~1TR'Y SA~VtI~LEOTL 0 ,o o ,o
% SEED p STE
lL1TT1C
~ AR1C OLEIC LI\OLI;IC
rwrrw~.rwwrrrrrrwwrrrrrw--rwwwrr
~ m.,r-rrr-r---------rrr-rr---r-rrrr-rrrrrr-rr.-..--
rrr r-rrrrr------..
rwrwrr-wrrrro-rwwrrwrwr.oNMR grams iG:O is:o is:l ~s:2
-m-
6778-3 oo--.vwYr-on--------mr----..w---o-r-wrwwwws--ww-wo------
----a-s-e-----w
6490 3403 3.3 I.0 83 9
3 5 9
3043 3.9 1.6 . .
6610-3 3I 83.5 9.5
34
3.3 1.1 83.5 l O.I
6077-1 3533
3.8 1.2 83.5 9.8
6754.2 3350
3.s 2.1 s3.s s.2
6099-1 1339
3.4 1.1 83.5 10.2
6761-4 3368
3.5 1.3 83.5 9.6
6777-2 3398
3.4 1. I 83.5 I0.3
6803-4 3457
3.7 1.3 83.5 9.9
6696-4 3216
3.4 1.0 83.5 9.9
6737-4 3296
3.8 1.4 83.4 9.6
6074-5 2724 3
5
. I.0 83.4 I0.0
6484-4 3028 3
6
. I.3 83.4 10.I
6511-2 3062
3.3 1.1 83.1 10.2
6552-4 3100
4.1 1.1 83.4 9.9
61293 2807 .
3.6 1.1 83.4 9.9
b5i9-3 3075
3,7 1.4 83,4 I0.2
6777-I 2090
3.4 1.1 83.4 10.6
6079-1 3535
3.5 1.0 ~ 83.4IO.U
6473-5 3012
3.8 1.8 83., 9.2
608?-1 3538
3.6 1.4 83.4 9,6
6102-1 3544
3.4 I.1 83.4 9.6
6297-2 2931
3.6 1.5 83.4 9.~
6701-2 ~ 3226
3.5 1.3 83.4 9.1
6657-2 3164 3
8
. 1.3 83.3 9.7
6218-5 2873 3 1 83
5 2 3
. . . 10.5
6077-3 2730 3.7 1 83 9
2 3 8
6009-2 2697 3.6 . . .
6915-3 1.1 83.3 I0.5
3243 3,3 I.2 83.3 9.3
6656-? 3I60
8041-1 3.9 1.5 53.3 g,7
l I35
3.4 1.2 83.2 10.5
6790-2 3430
3.8 I.2 83.2 10.1
6749-5 3333
3.3 L3 83.2 7.2
6497-3 3047
3.7 1.4 83.2 9.6
6789-S 3429
3.7 1.2 83.2 9.0
6743-3 3311
. 3.4 1.~ 83,2 9.0
620-1 3578
4,0 I.4 53.2 9.G
6906-3 3514 3
9
. 1.4 83.2 9,8
6695-2 3210 6
3
. 1.2 83.2 9.9
6114-4 2784 3
7
. I.5 83.2
6552-3 3099
4.1 1.3 83.2 9.7
6128-5 2805
..4 1.0 33.2 10.J
621 s--1 2$72
3.7 1.~ b3.2
6657-5 3167
3.6 1.2 53.2 9.9
6690-3
62 i 0-5 ZHS~ 3,'7 1
6065-1 3$26 7 I .3 n3.2 10.
3.7 I.2 83.2 I0.3
WO 91/11906 PCT/US91/00917
:.'~.,.
-102-
2~5~.~~~ ~,
1990 Olcic Safftorvcr Singlc Fow Results
OPEV TOTAL
FIELD
LA$ SEED OPEC' ~'o % ~'o ~'o
E'~'~'R'~' S'~
iYIPLEOxL %
__- ' SEED PALVIITrC STEARIC OLEIC LIVOLLIC
._____ _."
_......
~yi~-_ ._~Tams __".IG,p_.~S:a..........~5:~-_-Ig:Z
srrr..... rr.___._. ..-- ..._____..
.rrr.r_.-_.w.rr..._...._wr_-..._.-._
w ...-...__.__._.__..__-
___.._.._..w...._._www.wrr.rrrr.....--o._.r_....w_.._.
6883-2
3505 4.0 1.5 83.2 8.6
6913-3 3522
3.8 1.I 83.2 10.6
6205-4 2844
3.7 1.2 S3.I 9.6
6249-3 2908
4.2 1.3 83.1 9.5
6759-4 3364
3.4 1.3 83.1 10.8
6069-1 3529
3.5 1.2 83.1 10.0
6499-S 3057
3.7 1.4 83.1 9.8
6627-3 3144
3.6 1.2 83.1 9.7
6296-3 2928
3.2 1.5 83.1 7.8
6296-S 2930
3.1 1. I 83.1 10.4
6132-2 2814
3.5 1.0 83.1 10.4
6107-1 1347
3.4 I .1 83.1 10.3
6659-1 1973
3.3 I , 8 83.1 I 0. I
6218-1 3569 ~ ~
3.S 1.3 83.1 10.1
6697-3 3219
3.4 1.2 83.1 9.6
6656-5 3163
3.7 1,6 83.1 9.4
6381-2 2968
3.$ 1.2 83.1 9.8
6803-5 3458
3.7 1.2 83.1 10.2
620$-2 2846
3.6 1.3 83.1 9.7
687$-2 3487
4.0 1.4 83.1 10.8
6269-2 ' 2923
3.3 1.4 83.0 10.1
6130-S 2813
3.4 1.0 83.0 10.5
6298-1 3S8S
3.6 1.1 83.0 9.8
6133-3 ' 2819
3.~ 1.0 83.0 10.4
6098-3 2771
3.6 1.1 53.0 10. I
6610-4 3133
3.3 1.2 83.0 10,.5
6221-3 2575
3.7 1.3 83.0 I0.0
6915-S 3245
3.5 1.5 83.0 9.3
6744-3 3315
3.9 1.0 53.0 10.1
6126-S 2S01
3.6 1.0 83.0 10.5
6205-3 2843
3.7 1.2 83.0 10.0
6210-I 3565
3.3 1.0 83.0 9.2
6448-3 2982
3.4 1.0 83.0 10.3
6775-1 208 S
3.5 1.1 83.0 10.7
...... . . _ ,. . .. ~ . . . .. .... . . . .. . . . .. ... . ... ., .... . .
.,. .... ". ,.,. ....,_ ... . ., _...... ....>;, u...:...~. .. _ .< .
......_...~,-.:.,i:.;....,..
WO 91/11906 PCT/US91/00917
~.:5,..~ . , .. ._.)
. ' , ..
s
-103-
_, ;
..
~51435
LI~10LEIC LYSES
1990 I\CRIr.aSE
OIL QU.~LITY
W'~
OPEN TOTAL
FIELD LA$ SEED OPEW'c ~To c'o Rio
'
E~ SAi~IPLEOIL SEED PAL1IITIC OLEIC LINOLEIC
T8Y ~'o STEARIC
__.-L-__--__.Øh.--___.---H-___---_.Y.._ .__--...-.
--...-._.____-o-o__.------.__--..._.___-._-__.___..__._-.-m
._...-... ~11R grams 16:0 18:0 18:1 18:2
#._...... ...__._ ~
90-LI-1154-1_ .._.....__.-..-...-i -..-6 _.86
1178 ___.._....._5.4.__.___ 9......_.5 -'. 2-_-_-____.
90-LI-1094-11166 5.5 . . .
1.8 6 85
2 9
90-LI-I079-11161 5.1 1.6 . .
7 8S
0 7
90-LI-1205-11188 5.3 1 . .
7 6 85
6 7
90-LI-1152-I1176 5.4 . . .
1 7 85
8 2 6
90-LI-1180-I1183 S.4 . . .
2 7 85
0 1 6
90-LI-1060-11157 S.6 . . .
1 6 SS
7 5 3
90-LI-1080-11162 5.4 . . .
1 4 85
9 7 3
90-LI-1156-1, 1193 5.1 . . .
1 6 85
9 6 3
90-LI-1061-11158 5.2 . . .
1.7 6 2
9 85
90-LI-I117-I1170 5.6 1.7 . .
6 85
6 1
90-LI-1219-11191 S.6 I.8 . .
7 85
4 1
90-LI-1162-11179 5.6 1.9 . .
6 85
9 0
90-L1-11441 1175 5.6 1.9 . .
7.6 85.0
AVG 85.4
WO 91/11906 PGT/US91/00917
-104-
a~GOJ$ .~x~~
1989 Analyses
89b-
Oil
Quality
TOP LINOLEIC
~
' ~ ' ' LAB OPEN 'X'OTAL
. ~ ~FII~LD
~
EiYTR'Y SAh-IPLI OPEN PALIxITxC % OLEIC %
SEED
OIL SEED % STEARIC % zn~ozc
% ~
.-...,r_..---...~-~....--..-. ---.- -.-~.~ -.-- .~...~-.-
# # N1~IR 8rams 16:0 1$:0 18:1 15:2
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89B-1607-3 30.5 53 3.9 1.3 4.1
4549 90.7
S9I3-1607-Z454$ 30.5 53 3.7 I.3 4.4 90.6
89B-757-1 2521 30.8 l0I 3.6 1.6 3.7 90.5
89B-1397-3 4109 N/A N/A 4.0 6.0 90.4
89B-139?-1 3679 N/A N/A 3.9 1.4 4.3 90.2
89B-1604-4 4547 N/A 15 4.0 1.6 4.2 90.2
89B-7524-1 2490 I~'/A 8 3.6 1.4 4.2 90.2
89B-7557-5 2916 30.8 101 3.8 1.3 3.9 90.2
89D-1604-2 3934 N/A 15 3.8 1.5 4.5 90.1
89B- i 607-54651 30s 53 3.7 L3 4.9 90.1
89B-1 GOZ-44545 N/A 06 4.1 1.5 4.4 90.0
89B-7527-1 2493 N/A 9 4.1 1.1 3.8 90.0
89B-75633 2921 41.2 62 4.0 L1 4.1 90.0
89B-1608-1 3937 29.9 60 3.9 1.5 4.8 89.9
89B-1394-3 4065 N/A I2 3.6 1.6 4.9 89.8
89B-178-4 4513 N/A 08 4.3 LZ 4.3 89.8
89B-1602-2 4543 N/A 06' 4.0 1.5 4.8 89.7
89B-1607-4 4550 30.5 53 4.0 1.5 4.9 89.6
$9B-1 GOS-34553 29.9 60 3.8 i.s 5.1 89.6
89B-1610-2 3938 V/A 04 3.9 1.4 5.1 89.6
8913-7557-32914 30.8 101 3.7 ~ 1.5 3.4 S9.S
89B-1394-5 4067 h'/A 12 3.9 1.6 5.1 89.4
89B-1584-3 4517 38.5 411 4.5 1.4 5.0 89.4
89B-2097-1 4645 33.8 31 3.5 1.6 5.5 89.4
89B-2095-I 4646 N/A 8 4.4 L2 5.0 89.4
89B-1394-2.4064 N/A 1Z 3.6 1.5 5.6 89.3
$9B-1578-5 4514 NlA 08 4.4 1.2 4.6 89.3
89B-i 610-4x557 NIA 04 3.8 1.4 5.6 89.3
89B-?557-2 2913 30.8 101 3.7 1.6 4.3 89.3
89B-1397-4 4110 N/A N/A 3.9 1.8 S.1 89.2
8913-1578-i39I3 N/A O8 4.5 La 4.9 89.2
89B-IG08-Z 4552 29.9 GO 4.0 1.7 5.2 89.2
89B-7526-3 2$98 29.8 72 4.1 i.5 4.1 89.1
89B1G08-4 4554 29.9 60 4.0 1.5 5.6 88.9
S9B-1608-5 455 29.9 GO 4.1 1.2 5.8 58.9
89B-2091-1 4639 23.5 34 3.5 1.6 6.0 88.9
89B-7~2G-1 2492 29.8 72 4.I 1.5 4.8 88.9
89B-1602-3 4544 N/A OG 4.I L7 S.S 88.8
~
8nB-1610-3 4556 N/A 04 4.I 1.6 5.6 88.8
89B 1607- 3936 30.5 53 4.1 1.8 5.4 88.7
I
89B-2094-2 4642 34.5 22 3.8 L8 5.8 . 58.7
89B-757-4 2915 30.8 I01 3.9 1.6 4.2 88.7
39B-139.x-44066 N/A 12 3.8- 1.7 6.0 88.6
89B-139?-2 4108 h'/A N/A 3.8 1.8 5.7 88.6
S9B-752:x-52896 N/A 8 4.3 1.4 5.2 88.4
S9B-7526-2 2897 29.8 72 4.4 L3 :1.5 88.4
89B-IS78-3 4512 1~'!~~ O8 4.5 1.3 4.6 88.3
pace 1.
Sldt'3~'aZ'i i v i ~ ~~!'v7
WO 91/11906 PGT/US91/00917
-I05-
':: _..
.
1989 ~ : ~,~..;.w.:;.;-,
89b- ~: ; .
Oil ~
Qualit ~
Analyses
y IC
~ TOP LL'VOLE
FIELD LAB OPEN '~"OTAL
E'TRY SA;1'iPLE OPEi\ P aZ,,~C QLL1C
SEED %
O~ % SEED % STEARIC % LINOLEIC
._-......-... _-_-..
8~ms ls:o 18:1 18:2
16:0
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89B-727-2 2899 7
tr/A
4.4 L1 4.3 88.3
89B-7527-4 2900 ~T/A 7 4.8 L1 3 88
7 3
8913-1209-24018 39.8 1S5 S 1:6 . .
I 5 2
1 88
89B-1192-1 3498 45.8 121 . L7 . .
5 1 88
2 5 I
89B-1394-1 3677 N/A I2 . 7 . .
4.3 1 6 88
0 0
89B-7524-2 2893 ~I/A 8 4.4 . . .
1 3 87
3 S 8
89B-?524-3 2894 N/A 8 4,2 . . .
89B-894-4 3805 N/A ~'/A 5.2' 8 4 87
1 5 7
89B-l 194-34000 46.4 54 4.9 . . .
1.6 5 87
8 6
89B-1612-2 4558 36.I 243 4.4 1 . .
5 6 87
4 6
89I3-1602-13933 ~'/A 06 4.6 . . .
5 4 87
1 6 5
89B-1857-1 4237 5L4 3I3 4.8 . . .
L6 6 4
1 87
898-7346-3 2768 tt/A ~1/A 5.2 ~ 1 . .
5 4 4
3 87
89B-1209-S 4021 39.8 I8S 5.1 . . .
L8 5 87
9 3
89B-1612-S 4561 36.1 243 4.3 1.6 . .
5 87
5 3
CEY~1IAL 4022 5.2 1.3 . .
6 87
0 3
89B-1ZI0-I 3514 4L8 136 5.1 1.6 . .
3.3 87
2
89B-12II-4 4029 42.9 I59 4.9 1.8 6 .
I 1
87
89B-7346-4 276? N/A ~1/A 4.7 1.5 . .
S.5 87
I
89B-1212-I 3516 39.1 IG4 5.4 I.4 5.4 .
87
0
89B-1592-I 3925 26.0 314 4.6 1:4 6.7 .
87.0
89B-89;-3 3804 N/A ~'/A 5.4 L8 5.7 87.0
89B-1193-2 3995 45,0 162 a.0 1.7 5.8 86.9
89B-1204-3 4008 40.6 105 4.8 1.4 6.9 86.9
89B-1210-2 4023 41.8 I36 5.2 1.7 6.2 86.9
89B-7663-I 2526 41.2 62 4.2 1.4 6.3 86.9
898-1 l 3495 45.9 I78 4.7 1.3 7.2 86.8
8$- I
89B-1193-5 3998 45.0 162 5,2 1.8 6.2 86.8
8913-1592-54530 26.0 314 4.7 1.3 6.7 86.8
89B-Ii931 3499 45.0 162 5.2 i.8 6.1 86
6
8913-1195-34004 43.5 42 4.9 1.8 6.7 .
86
6
S9B- i 592-24527 26.0 314 4.8 1.6 7.0 .
86.6
89B-IG01-4 4541 32.5 231 4.3 1.5 7.6 86.6
89B-2102-1 4374 35.9 102 4.9 L? 6.5 86
6
89B-2126-I 4396 36.3 260 4.6 1.7 6.5 .
86.6
89B-74i7-I 2400 39.6 205 5.5 1.5 4.6 86.6
89B-1193-4 3997 45.0 I62 5.2 1.7 6.6 SG.S
89B-II96-1 3501 43:5 42 5.3 1.8 6.5 86.5
~
89B-1209-4 4020 39.8 185 3.2 1.6 6.4 86.5
&9B-1211-Z 4027 42.9 I59 S.6 1.6 6.3 86.5
89B-1x34-1 3917 38.5 41I 4.9 1.6 6.9 86.5
89B-I6I2-3 4559 36.1 243 5.1 1.8 6.4 8G.~
S9B= 89-3 II45 50.0 I76 5.1 1.9 5.9 86.5
89B-1184-3 3978 46.8 203 ~.3 1.7 6.6 86.4
F9B-1193-4 4005 43.5 42 5,0 i.6 6.6 86.4
89B-120:1-24007 40.6 105 5.2 1.G 6.3 86.4
89B-1211-3 4028 42.9 I59 5,2 8.4 86.4
plae Z.
~UB~Ta i ~3 c E ~~:e~'1
WO 91/11906 PCT/US91/00917
~~. y .' ~A ~~
. .
i~i~av3~':~:a.~..~
,. -106-
1989
89b-
Oil
Qualify
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FIELD LAB L1VOLEIC
OPEN
~
TOTAL
ENTRY SAIrIPLE Ol'E~t PAI.iV'IITIC OLEIC "
SEEP ~''o
C
IIIII--I- rI------UXL % STE~'1RIC c
1o ~'c LINOLEIC
SEED
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yy-II-o-~-II-II-----------
---I--osm-----
NMR grams IG:O 18:0 Ig:l J8:2
YIIII1I------
89B-121 -rsr W ---Irr-um-o-I--o-----------I
--..m ~------
41 3518 47.0 205 S
2
. 1.7 6.0 86.4
89B-1633-34578 38 260
4
. 4.7 I.7 6,1 36.4
89B-2029-14491 36 231
9
. 4.2 1.4 7.9 86.4
89B-895-1 3241 N/A N/A
4.5 1.9 5.9 SG.4
89B-1194-44001 46 54
4
. 5.3 2.0 6.5 SG.3
S9B-1194-54002 46 54
4
. 5.0 1.S ..2 36.3
89B-1210-34024 41 136
8
. 5.2 1.7 6.S 56.3
89B-1212-44033 39 164
1
. 5.4 1.S 6.4 SG.3
89B-1591-54526 29 206
0
. 4.5 L3 7.4 86.3
89B-1026-23818 45 21
9
. 4.9 1.6 7.3 SG.2
89B-1190-33988 46 115
6
. 4.9 1.6 7.2 g6,2
89B-1190-43988 46 II5
6
. 4.8 1.S 7.4 56.2
89B-1190-53990 46.6 115 4
9
. 1.4 7.5 86.2
89B-1192-43993 45.8 121 5
0
. 1.7 6.7 86.2
89B-1208-34015 40.8 175 5
2
. 1.5 6:3 36.2..
89B-1601-S4542 32.5 23i 4
5
. 1.4 7.; .56.2
89B-2i 4382 29.6 I49 4
10-1 7
. 1.7 6.2 X6.2
89B-789-S 1147 50.0 176 5 1
3 9
. . 5.8 S6.2
89B-82S-1 3179 52.5 224 5 1
1 4
. . 7,3 56,2
89B-8~J4-23803 N/A N/A 5 1
0 6
, . 7.1 56.2
89B-1192-33992 45.8 121 5 1
3 7
. . 6.4 86.1
89B-1194-23999 46.4 54 5
5
. 1,6 6.4 86.I
89B-i 195-54006 43.5 42 5
0
. 1.9 7.0 S6,1
89B-1204-13509 40 103 S
6 4
. . I,7 6.8 SG.:
89B1205-I 3510 38.6 23 5 1
3 8
. . 6.1 X6.1
89B-1208-44016 d0.8 175 5 2 6
2 0 8
. . . 56.I
898-1211-54030 42.9 I59 5 1 6
2 8 9
. . . 36.1
89BI223-1 3525 4$.5 139 5 1 7
1 6 3
. . . ~6.1
89B-1316-13613 X8.2 274 4 1 8
4 3 1
, . . 86.I
89B-1593-13926 45.0 320 5 1
0 4
. . 7.1 36.1
89B-1612-44560 36.1 243 4
4
. 1.7 ?.1 56.1
89B-2117-14389 43.6 94 4
8
. 1.6 6,5 56.1
89B-714I-I2092 38.4 168 5
0
. i.6 S.S 56,1
89B-894-1 3240 N/A N/A 5
0
. 1.8 7.1 56.1
89B-89,5-23807 1'/4 Nr/A 4 2
5 0
. . 6.1 S6.1
89B-1181-33970 42.2 264 4 1
8 6
. . 7.6 g6.0
89B-1195.24003 43.5 42 5
2
. 1.8 6.3 aG.O
8~B-1208-24014 40.8 175 5 I
3 7
. . 7.0 SG.O
89B-1212-34032 39.1 164 5 1
7 5
. . 6.? 86.0
8913-1856-14236 48.4 32S 4 I
8 I S
89B-2108-14380 37.2 17 . . ~.~ ~6
4 p
6
SIB-2122.14392 43.3 105 . 1,7 .
5
3
. 1.7 7.0 55.0
59B.2127-1d397 44.2 232 4
8
. 1.6 6.5 56.0
89B-2213-14659 42.8 58 4
4
. 1.5 8.1 X6.0
89B-7311-42763 44 ISS 4
4 6
. . 1.7 6.6 a6.0
59B-7448-12373 46.3 146 4
4
. 2.0 5.9 >6.0
s9B-7475-52882 44.4 272 ~
0
. 1.7 ~. 56.0
~
WO 91 / 11906 PCT/US91 /00917
. v .. ...~
-107-
1989
S9b-
Oil
Quality
.analyses
MELD ~ TOP
LAB LIVOLEIC
OPEN
ENTRY TOTrIL
S'1'~'IPLB P
SEED 1LVIITIC
OPEN ~'
: o OLEIC olo
.___ OIL % STE
% SEED ~RIC
~''b
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_....__.....-. 1
$9B .._......_.._._...-._._______..._.._______..-_.-.._,
lIB
._ .-___..____-__._..._..
- 3969 42.2 264 4
I-2 9
. 1.9 7.3 85.9
89B-I I $4-2 3977 46 203
8
. 5.0 1.7 7.5 S5.9
89B-11$9-2 3985 38 40
1
. 4.8 I,4 7.2 85.9
89B-1190- I 3497 46 I 1 S
6
. S.0 1.7 7.4 85.9
898-1208-I 3512 40 I73
8
. 5.6 1.7 6.0 85.9
89B-1214-2 4035 47 205
0
89B-1363-1 3654 . 70 4.8 1.6 7.6 85.9
40
1
. 8.1 85.9
898-1590-3 4521 23G 4.8 I,2
32
3
. 4.9 I,5 7.I 85.9
89B-1724-1 4152 39 359
3
. 4.4 1.S 8.3 85.9
89B-1903-I 4282 43 291
0
. 5.0 1.7 7.5 85.9
89B-2030-1 4492 38 366
2
. 5.0 1.6 7.1 g~,g
89B-7346-2 2765 N/A N/A '
5.4 1.7 5.7 55.9
89B-7346-3 2766 N/A N/A
5.0 1.8 6.2 85.9
89B-7475-2 2879 44 252
4
. 4.6 1.6 6.3 85.9
89B-934-3 1 i 49 I 23
80 7
. 4. L 8 7.4 85.9
89B-1183-1 3973. 42 267 8
0
. 5.2
89B-1184-4 3979 203 1.9 6.5 85.8
46
8
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89B-1186-I 49 1 230
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89B-1209-3 9
1
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89B-1316-2 4043 3$ 274
2
. 4.6 1.4 8.2 85;g
89B-1593-3 4532 45 320
0
. 5.0 I.6 7.5 8.5.8
89B-I596-1 3929 39 228
3
. S.0 I.7 7.6 85.8
R9B-1601-1 3932 32 231
5
. 4.7 I.6 8.0 83.3
89B-1632-1 3957 38 238
7
. 4.9 1.9 7.4 85.8
89B-1636-1 4073 39 215
5
. 4.5 85.8
89B-2027-1 4489 250 1.6 7.3
30.0 4
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. 1.5 6.7 BS.g
89B-2103-1 4375 41.9 lI4 4
9
. I.9 7.4 g5.8
89B-2153-I 4422 43.8 153 4
8
. 1.9 7.S a~.3
89B63G-1 3043 4~..1 237 5
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89B-G56-2 3836 44.1 237 4
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89B-7311-1 2247 44.4 155 s.o s5.8
4
6
. 1.4 6,~ g5.8
89ti-7473-3 2876 45.4 193 5
0
. 1.6 6.0 85.8
89B-7477-5 2887 39 205
6
. 5.3 1.7 S.3 g5,8
89B-7503-5 2892 31 215
3
. 4.7 1.G 6.8 85,g ,
89B-1026-3 3819 45 21
9
. 5.1 1.6 7.6 85.7
89B-1132-1 3447 4G 155
3
. 4,9 1.9 7.0 85.7
89B-1181-1 3488 42 264
2
. .~.4 1.6 7.7 85.7
. 5913-1192-2 3991 45 121
8
. 5.2 1.7 6.6 S5. 7
89B-12I2-2 4031 39 164
1
. 5.6 ~1.6 7.1 85.7
89B-1341-1 3633 37.5 89 6
1
. I.S 6,7 85.7
89B-1517-1 3857 39.6 2i7 5
0
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89B-1593-.~ 4533 45.0 320 4
6
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39B-1602-5 4546 N/A O6 4
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g9(3-1632-3 4574 38.7 2a8 :;
2
. 2.0 6.6 55.7
89B-1635-3 4612 40.5 186 4
6
. 1.9 6.3 35.7
g9B-1636-4 4617 .9.5 21 4
5 6
. . 1.6 6.8 55.7
89B-2107-1 4379 35.9 122 5
8
. 1.6 6.9 55.7
WO 91/1190b PCT/US91/00917
~~D5~.4~~
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89B-7475 .=
I
- 2398 44.4 252 G.0 1.5 4.9 85.7
89B-7477-42885
39.6 20S 5.:~ l .5 5.7 8 S.7
8913-1072-43822 48 193
3
. 5.1 1.7 6.9 85.6
89B-II84-13491 46.8 203 5 1
0 6
. . 7,8 85,6
89B-1186-43983 47.1 230 4.4 1 8 8S
8 2 G
89B-1210-S4026 41.8 136 5.2 . . .
1 6 85
7 8 6
89B-1488-13756 37.5 13~ 4.7 . . .
1 7 85
3 9 6
89B-1533-13872 38.3 112 4.8 . . .
1 8 85
4 1 6
89B-1592-44529 26.0 314 5.0 . . .
1 6 85
5 8 6
89B-1634-13959 37.8 19S 4.6 . . .
L9 8 85
0 6
89B-2002-I4466 42.1 59 4.8 2 . .
0 7 85
6 6
89B-609-2 1084 46.6 181 5.5 . . .
4 7 85
1 5 6
89B-609-3 108 46.6 181 5.3 . . .
1 6 85
5 9 6
89B-7139-22572 35.3 117 4.8 . . .
1 6 85
8 3 6
. . .
89B-7477-32884 39.6 205 5.5 1 5 .
5 6 85
6
89B-7587-12548 39.5 5I -~.7 . . ,
1 6 85
8 3 6
89B-1183-43975 42.0 ~67 5.1 , . .
1 7 5
6 9 85
89B-12053 4012 38.6 23 S.8 . . .-
7 6 85
1 9 5
89B-1209-13513 39.8 185 S.0 . . .
1 6 85
6 7 5
89B-1316-34044 38.2 274 ,7 . . ,
1 8 85
5 3 5
S9B-1341-3049 37.5 89 6.0 . . .
1.4 7 85
1 5
89B-I S22-I3861 39.4 94 ~.4 1.4 . .
7 85
3 5
89B-IS91-13924 29.0 206 .5.1 1 . .
8 7 85
6 5
89B-1637-14074 38.6 1G9 4.8 . . .
1.9 7 55
8 5
89B-1637-24619 3$.6 1G9 4.3 1.8 . ..
6 85
9 5
8913-607-51077 46.2 217 S.1 1.7 . .
7 85
6 5
89B-7167-22580 43.8 300 ,.7 1.9 . .
6 85
S 5
89B-7346-122S0 \/.~ \!~ S 2 1.8 . .
5 5
8 8
5
89B-7534-12499 38.9 I::B 4.6 2.0 . .
7 .
6 85
5
89B-7536-12501 ;6.3 223 5.0 1.S . .
6 85
8 5
89B-789-4 1 I46 50.0 17 6 5.6 1.9 . .
6 85
4 5
89B-885-1 3231 :;9.0 101 ~.2 I.~ . .
7 85
8 5
89B-1072-33821 45.3 198 5.1 1.6 . .
7 85
4 4
89B-1I32-33962 .~6.3 la5 :l.g 1.8 . .
6 85
7 4
89B-1186-33982 47.1 2~.0 T.9 1.7 . .
8 85
1 4
S9B-1189-13~9G 33.1 ~0 4.9 1.8 . .
7 85
9 4
89B-1190-23987 46.6 11~ ,.9 1.7 . .
1 4
8 SS
89B-1204-S4010 :10.6 105 5.9 1.4 . .
6 4
9 85
89B-1205-44013 3S.6 23 S.3 1.9 . .
6 85
9 4
89B-1214.34036 :17.0 20S =.9 I.6 . .
8.1 4
55
8903-1363-24056 40.1 70 ~ 1,4 g .
0 85
4
39(3-1637-34620 35.6 1 G7 ~.S 1 , .
7 6 .;
7 g5
S9B-1922-14298 =13.2 235 ' '' . . .
1.4 4 g5
8 4
S9B-1948-1432a 4?.0 270 4.7 I.S . .
8 55
1 4
59Q-21=il-14409 41.9 1Q~ .L9 1.9 . .
7 85
8 4
59B-7139-12091 35.3 ::' 4.5 1.9 . .
6.2 ,5.4
WO 9i /i 1906 PCT/US91 /00917
,.-f~. -i o 9- 2t~5~4~~..
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1989
89b-
Oil
Quality
Analyses
TOP C
~ LL\OLEI
FIELD LAB OPEN TOTAL
E~'1"RY SA1~I1'~r.E OPEN P:lLIItTIC%v OLEIC
SEED
OIL SEED % . STE:~.RxC % LINOLEIC
%
iiliwiH-wIIIHNIyIw111H1..YW rl-.WwYOrm-o----wN---------r-w-w------a.-soo-
-w----
-.In
'
# IrIR grams 1 G:0 18:4 18:1 18:2
h
.. w,r-------irw-w-iwwiiiwwwwwr-~Nuwiwrowan.w---n.-....-.-N-N....-.--...-.---
--.--..m
89B-7192
Z
- 2588 43.4 167 ~.0 1.4 6.6 85.4
89B7394-2 2868 49.4 S4 ~.0 1.6 6.7 85.4
89B-74621 2386 40.8 295 ,.8 1.6 6.5 33.4
89B-75632 2920 41.2 62 4.2 1.6 7.2 85.4
89B-7613-1 2570 44.3 44 4.3 1,7 7.1 85.4
89B-774-4 1112 49.3 190 ~.3 1.6 7.7 85.4
89B-851-1 3200 44.9 91 5.8 1.3 7.5 85.4
89B-909-4 1176 43.4 287 5,5 1.5 7.4 85.4
89B-917-1 3258 48.3 302 5.3 1.5 7.8 85.4
89B-917-4 3814 48.3 302 5.4 1.8 7.4 85.4
89B-1026-1 3351 45.9 21 4.7 1.4 7.1 85.3
89B-1072-2 3820 48.3 198 5.5 1.8 7.4 85.3
89B-1183-5 3976 42.0 267 5.3 1.4 7.5 85.3
89B-1192-S 3994 45.8 121 5.5 1.5 7.6 85.3
89B-IS94-1 3923 32.3 236 3.3 1.3 8.1 85.3
89B-1601-3 4540 32.5 231 4.7 1.4 8.6 85.3
$9B-1612-1 3939 36.1 243 S.I 1.8 7.7 8~.3
89B-1623-2 394$ 43.7 122 5.1 1.7 7.9 85.3
89n-1633-4 4579 38.4 260 4,6 1.9 7.0 85.3
$9132106-1 4378 40.3 ~ 64 5.3 1.6 6.9 85.3
89B-2112-1 4384 30.3 266 4.8 1.8 8.1 85.3
89B-2116-1 4388 43.8 I19 4.G 1.8 7.8 85.3
89B-2125-1 4395 31.9 200 5.0 1.5 7.7 85.3
89B-625-1 3013 45.1 291 5.4 1.4 7.6 85.3
89B-633-1 302I 45.9 255 3.4 1.2 7.6 85.3
89B-633-4 3830 45.9 2S5 S.6 1.4 7.7 85.3
89B-644-1 3030 44.1 2$$ 5.4 1.6 7.7 85.3
89B-656-4 3838 44.1 237 4.8 1.3 8.1 85.3
89$-7139-3 25?3 35.3 117 5.1 1.6 6.7 83.3
89B-7394-1 2324 49.4 54 4.9 1.6 6.4 85.3
~
89B-744.7 2372 37.6 262 4.? 1.9 6.fi 85.3
1
89B-7448-5 2863 46.3 146 4.6 1.9 G.8 85.3
89B-1193-3 3996 45.0 162 5.4 1.8 7.2 85.2
89B-1211-1 35I5 42.9 159 5.2 1.4 5.2 85.2
898-1212-5 4034 39.1 164 5.6 1.8 6.9 85.2
89B-1351-1 3643 49.5 27I 5,4 2.0 7.3 85.2
89B-13f~-1 3656 4s.8 s4 4.3 1.6 8.9 85.2
89B-1503-1 3843 4I:4 3I1 4.6 1.7 8.4 85.2
89B-IG:2-2 4573 38.7 238 ~.4 1.8 7.0 85.2
89B-1635-I 4072 10.5 186 4.9 1.9 7.0 85.2
89B-1877-1 42SG 47.3 GO 5.2 1.6 7.5 85.2
89B-1584-1 4265 43.0 18 5.4 1.4. 7.9 '85.2
8913-19:12-14328 40.0 261 4.6. ~ 1.5 8.3 85.2
89B-2096-1 4644 37.1 113 4.5 ~.0 7.8 85.2
89B-2128-1 4398 44.9 I 18 5.6 1.6 7.0 g5.2
89B-590-2 1069 46.4 158 5.3 1.6 7.4 85.2
$9B-7081-? 2461 41.1 27:1 4.6 1.7 7.j g5.2
page 6.
SU~STiTi)T° SE <~~~
WO 91/11906 PCT/US91/00917
-110-
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~~5~.~3~ ~ 1989 Analyses
89b-
OPI
Quality
. TO LIVOLEIC
FIELD LAB OrEV TOTAL
ENTRY SAMP Lr OPE\T PALMITi'C OLEIC
SEED %
OIL SEED % STEARIC % mNOi~Ic
%
-r---------r1-1t--r-11.1--r-r- y--..----w--..-r----ow-rnu----------
s--u----v-W -------
#
# 1~~IR grams iG:O 18:0 18:I 18:2
_ w~-
Ny.1.---"w~
-wos ~..IO--~r1..11---r----1-YN---a-y------0-11...--u
89B-?503
1
2471 3 L3 2I S 4.2 ' I.4 7.9 85.2
89B-826-1 3180
52.8 I37 S.I 1.7 8.0 85.2
89B-889-1 3235
50.2 220 4.9 i.s 8.4 sS.2
89B-890-1 3236 4G
3
. 235 S.3 1.6 7.S 83.2
898-894-:5 3806 N/A N/A S
2
. 1.9 6.2 85.2
89B-90'7-2 I164 48.5 231 5 1 6 85
4 9 7 2
$9B-1163-1 3471 47.3 91 . . . .
5.2 1 7 1
8 3 85
89B-1412-1 3687 42.8 108 4.9 . . .
1 8 1
6 3 8$
898-1488-4 4264 37.5 I2S 5.4 . . .
1 7 85
6 9 1
89B-1593-S 4534 45.0 320 4.9 . . .
1 7 8S
7 S i
89B-1624-1 3949 39.5 292 5.8 . . .
1 7 BS
6 5 I
89B-1633-1 3938 38.4 2G0 4.7 . . .
1 7 85
9 7 I
89B-2011-1 4475 39.8 130 5.2 . . .
1.5 8 I
2 8S
89B-2i0I-1 4373 39.2 249 5.4 LS . .
7 85
I 1
89B-2123-x 4393 35.0 lIl 5.4 1 . .
3 8 8~
2 1
89B-G08-2 1079 46.2 137 S.1 . . .
1.5 7 8~
7 1
89B-633-3 3829 45.9 255 3.1 L6 - . .
8 85
3 1
89B-71231 2077 36.4 25 4.7 1.6 . .
7 85
1 I
89B-718$-4 2132 47.6 255 S.2 2.3 . .
S.7 85
1
8913-7311-3 2762 44.4 155 4.5 1.6 7 .
6 85
1
89I3-755$-1 2522 35.9 36 4.4 I.G . .
7 85
5 1
89B-8905 3802 46.3 255 5.1 i,6 . .
7 1
7 85
89B-1072-1 3393 48.3 i98 5.4 1.5 . .
2 85
7 0
8913-1183-2 3490 42,0 2G7 4.9 1.7 . .
7 85
9 0
89B-1186-5 3984 47.I 230 4.7 1.8 . .
8 85
5 0
89B-1214-4 4037 47.0 205 5.3 1.7 . .
7 85
6 0
89B-i31 G-5 4046 38.2 274 5.2 1.5 . .
8.4 8~
0
89ti-1589-1 3922 40.3 120 6.6 1.6 6 .
8 85
0
89B-1613-1 3940 39.5 207 4.7 1.6 . .
8 83
6 0
89B-1635-5 4614 40.5 i 86 4.9 1.9 . .
7.1 85
0
89B-1675-1 4107 46.4 200 S.S 1.8 7 .
3 85
0
89B-1885-1 4266 39.2 374 5.2 1.8 . .
?.S 83
0
89B-1945-1 4321 46.1 299 4.8 I.7 8 .
6 55
0
89B-1957-1 4333 49.0 300 S.0 2.0 . .
7.8 85
0
89B-2028-i 4490 36.3 216 4.7 1.8 7 .
7 8~
0
89B-2092-1 4640 41.7 26 4.8 1.8 . .
6 85
? 0
89B-2124-1 4394 44.2 74 4.3 1.6 . .
? 8a
fi 0
89B-2145-I 4414 45.0 84 4.8 L9 . .
8 8~
3 0
8913-? 192-12135 43.4 167 S. I 1.S . .
7 85
3 0
89E-7299-i 2237 34.3 58 4.6 i.8 . .
9 83
6 0
89B-7x73-1 2396 45.4 I93 5.0 1.9 . .
3 55
6 0
898-1498-1 2457 38.3 271 5.2 1.5 . .
8 85
6 0
59B-7498-1 2886 38.3 271 S.2 ~ 1.5 . .
6 85
8 0
59B-7501-2 2459 35.7 I50 4.8 1 . .
8 4 5~
? 0
89B-834-1 3188 43.3 149 5.2 . . .
1 8 85
G 3 0
$9B-8G3-1 3211 44.8 74 5.9 . . .
1 7 85
9 3 0
89B-9GG-1 3298 47.7 23 5.4 . . .
2.1 ?.5 85.0
page 7.
~4IIrt~Tt'T'~T. ~ ~;1~~~ i
WO 91/11906 PCT/US91/00917
.. -111- ~'~~~'~~ ~~'~ ~~_ . .. _.
PROCESSING
The safflower seeds of the present invention may be
processed commercially by being ground to a typical size for
oil processing. The ground-seed composition is then cooked
in a sealed vessel for 1 hour at 130'C. Extraction is then
performed in a Butt-extractor, using commercial grade hexane
as the solvent for a period of 4 hours. The hexane treated
ground composition is then reground and returned to the
extractor for an additional 4 hours wherein a second extrac-
tion step is conducted using the aforementioned conditions.
The solvent is then removed from the miscella, first by
distillation to a pot temperature of 75'C and finally into a
roto-vac apparatus under reduced pressure of 1.32 KPa (lOmm
mercury pressure).
The resulting crude extracted oils were each treated
with sufficient 16' Baume sodium hydroxide solution to
neutralize the free fatty acids plus an additional 1.8% of
the sodium hydroxide based on the total oil weight for 5
minutes at 65'C under moderate agitation. The heavy soap
phase was separated by centrifugation. Residual soap and
impurity removal is accomplished by washing with approximate-
ly 15% by volume of water for 5 minutes at 90'C, followed by
bleaching the clear oil obtained by centrifugation with 1% by
weight acid activated earth at 1.3 to 2.6 KPa (10-20mm) and
95°C for 10 minutes. The activated earth is separated by
suction filtration.
The AOM determinations may be conducted pursuant to the
American Oil Chemists Society (ROCS) method CD12-57. The AOM
content of the high oleic safflower oils described herein is
at least about 60 hours, preferably at least 70 hours and
most preferably greater than 80 hours in the absence of an
antioxidant. If antioxidants are used the AOM values are
even greater.
Safflower oil may be identified by a unique sterol.
Safflower plants are believed to contain delta-7 stigmasterol
which is not believed to be found in other plants. The
safflower plants of the present invention are also unique in
WO 91/11906 PCTlUS91/00917
:a >w .._
i~:~~~. ~ "~::~ -112-
their ability to fix nitrogen from fertilizer below the level
of other plant species. Thus the safflower may be planted on
a previously fertilized field and make use of nitrates at
lower soil levels. Not only is the fertilizer effectively
used~but nitrate contamination of ground water is reduced.
While the invention has been disclosed in this patent
application by reference to the details of preferred embodi-
ments of the invention, it is to be understood that this
disclosure is intended in an illustrative rather than in a
limiting sense, as it is contemplated that modifications will
readily occur to those skilled in the art, within the spirit
of the invention and the scope of the appended claims.
