POLYNUCLEIC ACIDS AND PROTEINS FROM A PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS AND USES THEREOF

ACIDES POLYNUCLEIQUES ET PROTEINES OBTENUS A PARTIR DU VIRUS DU SYNDROME DYSGENESIQUE RESPIRATOIRE PORCIN ET UTILISATIONS DE CES ACIDES ET DE CES PROTEINES

English Abstract

The present application for the grant of a patent concerns
a compact light liquid separation plant with an integrated,
2-part sludge trap (1), a light liquid collection chamber
(14) above the separation chamber (16) and a fine
separation chamber (19), with an inlet closure (31) with
integrated overfill protection. The essential features are:
self-contained light liquid outflow in which the surface is
free of light liquid when at rest; inlet closure with
integrated overfill protection for three purposes with a
float; extended stay in the separation and fine separation
chamber by a vertical passage arrangement.

French Abstract

Cette invention se rapporte à une préparation purifiée contenant un acide polynucléique codant au moins un polypeptide choisi dans le groupe constitué de protéines codées par un ou plusieurs cadres de lecture ouverts d'une souche d'Iowa du virus du syndrome dysgénésique respiratoire porcin, des protéines homologues de celles codées par un ou plusieurs cadres de lecture ouverts, des régions antigéniques de ces protéines qui ont une longueur d'au moins cinq acides aminés et qui stimulent efficacement la protection immunologique dans un organisme hôte porcin contre une exposition ultérieure à un isolat de virus du syndrome dysgénésique respiratoire porcin et des combinaisons de celles-ci, dans lesquelles des acides aminés non essentiels pour l'antigénicité peuvent être substitués à des fins conservatrices. Cette invention se rapporte également à un polypeptide codé par un tel acide polynucléique, à un vaccin contenant une quantité efficace d'un tel acide polynucléique ou d'une telle protéine, à des anticorps qui se fixent spécifiquement à un tel acide polynucléique ou à une telle protéine, à des procédés pour produire ces acides ou ces protéines, et à des procédés permettant de créer une réaction immunologique efficace contre le virus du syndrome dysgénésique respiratoire porcin, pour traiter des porcs infectés par un tel virus et pour détecter ledit virus.

Claims

14
Claims
1. A light liquid separation plant for the separation of
light liquid/heavy liquid mixtures comprising a one-
part or multi-part sludge trap (1) with or without an
inlet closure (31) and an integrated overfill
protection (47), a separation wall (3), an inlet wall
(11) with an overflow edge (12) to an inlet channel
(13), a light liquid collection chamber (14) located
in the upper part of the following separation chamber
(16), a light liquid outflow (6), a circulation
channel (20) having a flow-technical means (slot) (18)
in the lower part, a fine separation chamber (19)
having also a flow-technical means (perforated plate)
(18') in its bottom part, an outflow channel (26)
having in the region of the outflow bottom (21) a
means for height adjustment (26'), and a light liquid
storage device (15), characterized in that the
overflow edge (4) of the separation wall (3) is
located slightly below the inlet bottom (2'), the
overflow edge (12) of the inlet wall (11) is located
slightly lower than the overflow edge (4) of the
separation wall (3), the overflow edge (20') at the
circulation channel (20) is identical with the height
of the outflow tube bottom (21), the overflow edge
(28') of the outflow wall (28) is located slightly

below the upper edge of the inlet tube (2), the
outflow tube bottom (21) is located below the water
level (23) in the rest condition for such a distance
as made necessary by the density of the light liquid,
for instance with a density of 0,85 for 150 mm below
the water level (23) in the rest condition, and that a
means (26') is located in the region of the outflow
bottom with which the height of the outflow tube
bottom (21) is determined by adjustment of the
overflow edge (262), for instance with a density of
0,90 for 100 mm below the water level (23) in the rest
condition.
2. The light liquid separation plant according to claim
1, characterized in that an adjustment valve (263) (or
a replaceable cross-section trottle) is located at the
device (26') in order to guarantee an overflow of the
overflow edge (28') of the outflow wall (28) with a
very low throughflow, and that the height of the
outflow tube bottom (21) is determined with the
overflow edge (262).
3. The light liquid separation plant according to claim
1, characterized in that the separation chamber (16)
and the fine separation chamber (19) are formed in
such a manner that the total height, the surface of
the separation chamber (16) and of the fine separation
chamber (19), the drain cross-section within the flow-
technical means (18) and (18') and the volume of the
liquid throughflow are adjusted with respect to one
another such that, in response to the throughflow, a
flow rate of below 2 cm/s is maintained in the
separation chamber (16) and the fine separation
chamber (19).

16
4. The light liquid separation plant according to claim
1, characterized in that the flow-technical means (18)
of the separation chamber (16) is formed in such a
manner that, dependent on the reduction of the free
throughflow, a uniform small sink rate is maintained.
5. The light liquid separation plant according to claim
4, characterized in that the fine separation chamber
(19) has a flow-technical means the apertures thereof
being uniformly distributed and dimensioned such that
the sink rate is below 2 cm/s.
6. The light liquid separation plant according to claim
1, characterized in that the flow of the light
liquid/heavy liquid mixture is directed in such a
manner that three overflow edges are generated, namely
1. the overflow edge of the water level (23) in the
rest condition, 2. the overflow edge at the lower
inlet of the light liquid collection chamber (14), and
3. the overflow edge of the heavy liquid lowering in
the separation chamber (16).
7. The light liquid separation plant according to claim
1, characterized in that a combined light liquid
outflow (6) is installed, the outer walls thereof
reaching up to the upper edge of the separator and the
two light liquid outflows (9) and (10) thereof being
separated by an intermediate wall (7), that the light
liquid outflow (6) for the light liquid collection
chamber (14) has a tube (10) adjustable in its height
and emanating from the light liquid collection chamber
(14), that the light liquid outflow (10) is defined
for the outer region or for the light liquid of the
whole surface and has a downwardly directed overflow
edge (8) slightly below the water level (17) in

17
operation, by which the thickness of the light liquid
layer is limited, that a light liquid outflow tube (9)
adjustable in its height is installed on the
intermediate bottom (6') located slightly above the
overflow edge (8), that both outflows (9) and (10)
extend to the light liquid storage device (15), and
that in operation after the collection of the
function-dependent light liquid volume all the
additional light liquid is drained into the light
liquid storage device (15) through the two light
liquid drain tubes (9) and (10).
8. The light liquid separation plant according to claim
7, characterized in that the overflow edge (12) within
the inlet channel (13) is located as deep under the
outflow tube bottom (21) as it corresponds to the
light liquid column below the water level of the
smallest density, that the outflow tube bottom is
located as deep under the water level (23) in the rest
condition as it corresponds to the light liquid column
over the water level with the smallest density, and
that both light liquid columns below and above the
water level correspond to the height of the function-
dependent light liquid column so that the light liquid
of the outer surface with the exception of the
function-dependent light liquid is received by the
light liquid storage device (15) after the
interruption of the inflow when the water level (17)
in operation sinks to the water level (23) in the rest
condition.
9. The light liquid separation plant according to claim
1, characterized in that the float (49) is located in
a dry float housing (48) and triggers the inlet
closure (31) by the inflow of heavy liquid through the

18
heavy liquid inlet tube (51) or the inflow of light
liquid through the light liquid inlet tube (50) only
if the maximum level of the heavy liquid or light
liquid is exceeded.
10. The light liquid separation plant according to claim
1, characterized in that the light liquid outflow tube
(10) consisting of transparent material (glass or
plexiglass) is adjusted with respect to the heavy
liquid inlet tube (51) such that the inlet closure
(31) is triggered by the inflow of heavy liquid if the
end of the light liquid column below the water level
becomes visible in the light liquid outflow tube (10).
11. The light liquid separation plant according to claim
1, characterized in that the closure member (34) of
the inlet closure (31) consists of the portion (34) of
a sphere which is connected to an outer lever (43) by
means of a device with a cross hinge (35) with axial
play and by means of an articulated shaft (39), the
outer lever bringing the closure member (34) again in
the position "open" after triggering.
12. The light liquid separation plant according to claims
10 and 11, characterized in that a bottom plate (40)
with a flow aperture is located in the range of the
water level within the housing (32) and has a sealing
surface (41) provided with a two-dimensional seal
having at the flow opening a small projection so that
the closure member lays on the sealing surface (41)
automatically and uniformly.

Description

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2 1 9 ~ 461
Li~ht Liquid Separation Plant
Specification
With the present patent application a light liquid
separation plant with integrated sludge trap, vertical
upward and downward passage, continuous light liquid
outflow, at least one separation and fine separation
chamber, inlet closure and integrated overfill protection
is described.
Conventional light liquid separators are provided with a
sludge trap which is connected to the separator by means of
a tube connection. The mixture consisting of sludge of all
kinds and of light liquids is fed into the sludge trap.
Within the sludge trap turbulences are generated by the
inflow through an inlet tube, wherein fine sludge and light
liquid are entrained into the separator and partly into the
sewage system by the flow. This entrainment is favoured by
the fact that turbulences are generated within the
connection to the separator by increased velocities which
cause an intensive mixing.
By the integration realized by the invention and described
in the following the sludge trap (1) gets the object to
take part in the functions of the separator, i.e. to keep
back a portion of the light liquid which is as large as

~1~1461
possible in addition to the coarse and fine sludge and thus
to unburden the separator and to prevent that the light
liquid already deposited on the surface prepares an
emulsion with the heavy liquid when entering the separator.
The surface of the sludge trap extends to the separation
wall (5) of the fine separation chamber (19). Here, the
largest portion of the light liquid, namely about 98 ~, is
retained on the highly increased water level and is
discharged into the integrated light liquid storage device
(lS) through the continuous light liquid outflow tube (9),
as subsequently described.
A separation wall (3) is disposed within the sludge trap
(1) and thus divides the same into a coarse sludge
separation chamber and a fine sludge separation chamber.
The separation wall (3) formed as overflow edge (4) gives
the subsequent fine sludge separation chamber optimum
relaxation and favours the retention of the fine sludge.
The inlet wall (11) formed as drain wall with overflow edge
(12) replaces the head wall of the separation chamber and
the inlet and outlet tube. Furthermore, by this inlet wall
(11) the whole water level of the sludge trap part (1)
becomes undisplaceable so that 98 ~ of the inflowing light
liquid are discharged into the light liquid storage device
(15) by means of the continuous light liquid outflow tube
(9) or the light liquid collection chamber (14).
In this manner the sludge trap (1) becomes the main
component of the light liquid separation plant.
The sludge trap (1) with the vertical inlet channel (13)
and the light liquid collection chamber (14) are integrated
with the separation chamber (16) in such a manner that the

~_ 3
~19i~61
light liquid is separated from the inflowing light
liquid/heavy liquid mixture already at the inlet tube (2)
and collects on the water level during operation (17).
The light liquid entrained with the flow is fed into the
separation chamber (16) through the vertical inlet channel
(13). The liquid volume is lowered with an extremely slow
uniform velocity by a flow-technical means (18) located in
the bottom portion.
In order to reach a duration of stay as long as possible
the separation chambers (16) and (19) are provided with a
circulation channel (20) in vertical direction. The
vertical downward velocity with nominal throughflow is to
have a value below 2 cm/s. The cross section or the surface
of the separation chambers (16) and (19) is determined by
the volume of the flow. The light liquid sinking with a
duration of stay which is as long as possible ascends in
the light liquid collection chamber (14) and collects at
the water level of the outflow bottom (22). With a density
of 0,85 a light liquid column of 15 ~ over the water level
and of 85 ~ below the water level, which would develop if
no light liquid were present, is formed. The water level
itself is displaced by the light liquid in accordance with
the density of the same.
If, in the present case, one emanates from the fact that
1 ~ is 5 mm, the light liquid column over the water level
will be 75 mm and below the water level will be 85 x 5 =
425 mm. The distance between the lower overflow edge (13')
in the vertical inlet channel (13) and the water level (23)
in the rest condition is 75 + 425 = 500 mm.
Of course, in this case nothing would be discharged.

_ 4
21ql461
However, if the whole light liquid heigth of 500 mm is only
499 mm, the whole volume in the plant is discharged into
the light liquid collection chamber (14), with the
exception of the function-dependent volume. In other words,
the volume of a heigth of 500 mm minus a heigth of 499 mm =
1 mm has been also discharged and now 499 mm instead of 500
mm are retained by the overflow edge.
In this situation the operation of the plant is started.
The water level (23) in the rest condition increases to the
water level (17) in operation. The increasing water level
presses the content of the light liquid collection chamber
(14) into the light liquid storage device (15). The
function-dependent light liquid within the inlet channel
(13), together with the water level (23) in the rest
condition, increases up to the water level (17) in
operation and is distributed on the surface.
Within the light liquid outlet tube (10) only the function-
dependent volume remains, i.e. the light liquid column
below and above the water level.
All the light liquid flowing in in the operation phase is
continuously discharged into the light liquid storage
device (15) beyond the function-dependent volume within the
light liquid outlet tube (10) so that the light liquid
collection chamber (14) is always receptive for the next
rest condition.
During the operation simultaneously additional light liquid
from the inflowing light liquid/heavy liquid mixture
collects on the water level (17) in operation for the
formation of a function-dependent light liquid layer. If
this layer reaches the downwardly directed overflow edge

~`- 2191461
(8) at the light liquid outflow (6) it flows in the light
liquid discharge tube (10) through the light liquid
discharge tube (9) to the light liquid storage device (15)
after collection of the light liquid column below and above
the water level (17) in operation. The overflow edge (8)
has the ~same function as the overflow edge (12) within the
inlet channel (13).
Apart from a possible light liquid film on the surface of
the fine separation chamber (19) in the rest condition only
the function-dependent light liquid in the inlet channel
(13) in front of the light liquid collection chamber (14)
exists.
This function-dependent light liquid volume can be also
drained into the light liquid storage device by the drain
valves (24). If it is drained the light liquid level starts
with a level of 0 at each start of operation.
If the function-dependent light liquid is not drained the
same ascends to the surface with rising water level (17) in
operation and distributes there.
During operation the drain valves (24) have to be closed.
The drain valves (24) are arranged on account of the
following reason on the water level of the drain bottom
(22) and the outer wall (25) in the light liquid storage
device (15): The water level of the drain bottom (22) is
decisive for the light liquid level above and below the
water level. Within the plant the light liquid distributes
on the water level, for instance with a density of 0,85
with 15 : 85 or 75 mm over the water level and 425 mm below
the water level. In other words, if 15 ~ are drained over
the water level 85 ~ are drained below the water level.

6 2 1 9 I 461
With a density of 0,90 10 ~ over and 90 ~ below the water
level result.
In this manner the drain valves (24) are correct for any
higher density.
The situation is different with regard to the light liquid
outflow within the separation chamber (16): If one wishes
to determine the light liquid outflow below the water level
one has to always emanate from the light liquid column
above the water level, for instance with a density of 0,85
the light liquid column over the water level is 75 mm and
the light liquid column below the water level is 75 : 0,15
x 0,85 = 425 mm. With a density of 0,90 75 : 0,10 x 0,90 =
675 mm.
As one can see the light liquid column below the water
level with a density of 0,90 is 675 mm, i.e. 250 mm more
than with a density of 0,85.
If in this case nevertheless light liquids of higher
densities already drain at 425 mm below the water level,
the reason for this can be seen in the fact that only a
light liquid column of 47 mm over the water level is
necessary when reaching a light liquid column of 425 mm
below the water level, i.e. 40 : 0,10 x 0,90 = 423 mm.
Since small production tolerances cannot be avoided a small
deduction is carried out from the planned light liquid
outflow below the water level.
Light Liquid Separator
The passage of the light liquid/heavy liquid mixture is
shown by arrows. It flows into the sludge trap (1) through

7 21 ~il 461
the inlet closure (26) and distributes over the whole width
of the sludge trap (1). Within the sludge trap (1) the
solid heavy substances sink and collect on the bottom while
the rising light liquid collects on the water level (17) in
operation wherein, in accordance with the light liquid
density, the water level is displaced by the light liquid,
for instance with a density of 0,85 with 15 ~ over and 85
below the water level which would be generated if no light
liquid were present, i.e. water level independent of
throughflow plus variations dependent on throughflow. 100
of the throughflow including about 2 ~ of the inflowing
light liquid are introduced into the separator itself.
From the sludge trap (1) the liquid flows into the light
liquid collection chamber (14) through the inlet channel
(13) wherein the remaining light liquid deposits upwardly
while the duration of stay of the heavy liquid is prolonged
by means of a flow-technical device (18) within the
separation chamber (16). The liquid flows into the fine
separation chamber (19) through the circulation channel
(20). Here, another extreme slowing-down of the sink
velocity is realized by the flow-technical device (18')
arranged in the bottom portion. A kind of opposite flow
separation is caused by this vertical flow wherein
simultaneously a mutual flocking of the separating light
liquid droplets within the vertically arranged fine
separation chamber (19) and of the separating light liquid
droplets of the inflowing light liquid/heavy liquid mixture
occurs.
The lower this fine separation chamber (19) is arranged the
higher the water pressure becomes so that also the smallest
light liquid components are pressed out.
If necessary, this fine separation chamber (19) serves for

_ 8 2~ 9 ~ 461
the reception of coalescence material in order to satisfy
even the highest requirements.
The purified heavy liquid flows through an additional
opening (27) at the lowest point of the fine separation
chamber (19) over the overflow edge (30) of the drain wall
(28) into the outlet (29).
Inlet Closure with integrated Overfill Protection
Normally, conventional light liquid separators are provided
with an automatic closure which closes the heavy liquid
outflow at a maximum light liquid level. However, in this
case the inflowing liquid is banked and presses the light
liquid out of the storage device or the separation chamber
into the ground. For removing this disadvantage the German
industrial standard 1999 has been changed in March 1989.
With an inlet closure such banking effect within the
separator is excluded.
An inlet closure works with a surface float while the
conventional closure at the outflow is provided with a
boundary float. This has to be lighter than heavy liquid
and heavier than light liquid, i.e. with a surface float
the weight of the float is additionally present in contrast
to the boundary float. Therefore, the cited inlet closure
has been developed for the described overfill protection.
According to the light liquid separation plant described
here a dry float housing ~48) is directly mounted at the
housing (32) of the inlet closure (31). A float is located
within the float housing at the heigth of the water level
(23) at rest.

-- 9 21~146~
The light liquid/heavy liquid mixture flows into the
housing (32) through the inlet tube (2) and leaves the
housing through the outflow tube (33) with opened closure
member (34). The closure member (34) is provided with a
device with cross hinge (35) the bolt of which being
mounted rotatably and axially displaceably crosswisely in
U-profiles. The lower profile (36) is connected to the base
plate (40) by means of a lever bolt (38) emanating from an
articulated shaft (39). In this manner the closure member
(34) is rotatable and axially displaceable in all
directions. The articulated shaft (39) extends outwardly
through the housing wall (42) (shaft seal) and is fixedly
connected to a lever (43). The closure member (34) is
permanently maintained in the position "open" through this
lever (43) by means of a triggering lever (44). The
triggering lever (44) is pivotable by a movable suspension
(45) and rests within a slot guide (46) above the float
housing (48) of the overfill protection (47) and terminates
in front of the lever (43) which holds the closure member
(34) open. If the heavy liquid or light liquid exceeds the
maximum level this heavy liquid or light liquid flows into
the dry float housing (48). The rising float (49) lifts the
triggering lever (44) and releases the closure member (34)
thereby. The closure member (34), after its triggering,
falls onto the sealing surface (41) cushioned by the water
level. In doing so the closure member (34) is centered and
is pressed against the sealing surface by the inflowing
light/heavy liquid.
Overfill Protection
This overfill protection is a protection with respect to an
uncontrolled and unexpectable overfilling and is to also
overcome human failure. Especially, foreseeable
overfillings are supervised and prevented by this

2~91461
protection in order to exclude contaminations of soil,
surface water and ground water by mineral oil and other
light liquids. This device makes the light liquid
separation plant inoperative by closing the inlet closure
(31).
According to the overfill protection (47) the overflow
edges of the light liquid inlet tube (50) and the heavy
liquid inlet tube (51) are separately disposed at the float
housing (48) in such a manner that an elevation of 1 mm is
sufficient for closing the inlet closure (31). By this,
three objects are achieved in a reliable manner, namely the
prevention of:
A the overfilling of the heavy liquid portions,
i.e. the sludge trap and separation portion,
B the overfilling of the light liquid storage
device,
C the inflow of heavy liquid into the light
liquid storage device.
In the drawing an example of the invention is shown. Of the
drawing
Figure 1 shows a longitudinal section of the light
liquid separation plant,
Figure 2 shows a cross section of the light liquid
separation plant,
Figure 3 shows a top view of the light liquid
separation plant,

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2191461
Figure 4 shows a longitudinal section of the inlet
closure with integrated overfill protection,
Figure 5 shows a cross section of the inlet closure
with integrated overfill protection and
Figure 6 shows a top view of the inlet closure with
integrated overfill protection.

2 1 9 1 46 1
Liqht Liquid Separation Plant with Inlet Closure and
integrated Overfill Protection
List of Reference Numbers
1. Sludge trap
2. Inlet tube
2' Inlet tube bottom
3. Separation wall
4. Overflow edge of member (3)
5. Separation wall to the fine separation chamber,
member (19)
6. Light liquid outflow
6' Intermediate bottom of member (6)
7. Intermediate wall of member (6)
8. Overflow edge of member (6)
9. Light liquid outflow tube for continuous outflow
10. Light liquid outflow tube for the outflow from
light liquid collection chamber, member (14)
11. Inlet wall
12. Overflow edge of member (11)
13. Inlet channel
13' Lower overflow edge of member (13)
14. Light liquid collection chamber
15. Light liquid storage device
16. Separation chamber
17. Water level in operation
18. Flow-technical means of member (16)
18' Flow-technical means of member (19)
19. Fine separation chamber
20. Circulation channel
20' Overflow edge of member (20)
21. Outlet tube bottom
22. Water level of outlet tube bottom, member (21)

~191461
23. Water level in rest condition
24. Drain valves
25. Outer wall to the light liquid storage device,
member (15)
26. Outlet channel
26' Device for height adjustment
262 Overflow edge of member (263)
263 Adjustment valve of member (26')
27. Opening to the drain wall, member (28)
28. Drain wall
29. Outlet
30. Overflow edge of member (28)
31. Inlet closure
32. Housing
33. Outlet tube
34. Closure member
35. Device with cross hinge
36. Lower profile
37. Upper profile
38. lever bolt
39. Articulated shaft
40. Base plate
41. Sealing surface with two-dimensional seal
42. Housing wall
43. Lever
44. Triggering lever
45. Suspension
46. Slot guide
47. Overfill protection
48. Float housing
49. Float
50. Light liquid inlet tube
51. Heavy liquid inlet tube

Representative Drawing