Note: Descriptions are shown in the official language in which they were submitted.
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WO 02/20756 PCT/USO1/27297
SECRETORY MOLECULES
TECHNICAL FIELD
The present invention relates to secretory molecules and to the use of these
sequences in the
diagnosis, study, prevention, and treatment of diseases associated with, as
well as effects of
exogenous compounds on, the expression of secretory molecules.
to BACKGROUND OF THE INVENTION
Protein transport and secretion are essential for cellular function. Protein
transport is
mediated by a signal peptide located at the amino terminus of the protein to
be transported or secreted.
The signal peptide is comprised of about ten to twenty hydrophobic amino acids
which target the
nascent protein from the ribosome to a particular membrane bound compartment
such as the
15 endoplasmic reticulum (ER). Proteins targeted to the ER may either proceed
through the secretory
pathway or remain in any of the secretory organelles such as the ER, Golgi
apparatus, or lysosomes.
Proteins that transit through the secretory pathway are either secreted into
the extracellular space or
retained in the plasma membrane. Proteins that are retained in the plasma
membrane contain one or
more transmembrane domains each comprised of about 20 hydrophobic amino acid
residues. Proteins
~ o that are secreted from the cell are generally synthesized as inactive
precursors that are activated by
post-translational processing events during transit through the secretory
pathway. Such events include
glycosylation, proteolysis, and removal of the signal peptide by a signal
peptidase. Other events that
may occur during protein transport include chaperone-dependent unfolding and
folding of the nascent
protein and interaction of the protein with a receptor or pore complex.
Examples of secretory proteins
25 With amino terminal signal peptides are discussed below and include
proteins with important roles in
cell-to-cell signaling. Such proteins include transmembrane receptors and cell
surface markers,
extracellular matrix molecules, cytokines, hormones, growth and
differentiation factors, neuropeptides,
vasomediators, ion channels, transporterslpumps, and proteases. (Reviewed in
Alberts, B. et al.
(1994) Molecular Biolo~y of The Cell, Garland Publishing, New York NY, pp. 557-
560, 582-592.)
3 o G-protein coupled receptors (GPCRs) comprise a superfamily of integral
membrane proteins
which transduce extracellular signals. Not all GPCRs contain N-terminal signal
peptides. GPCRs
include receptors for biogenic amines such as dopamine, epinephrine,
histamine, glutamate
(metabotropic-type), acetylcholine (muscarinic-type), and serotonin; for lipid
mediators of inflammation
such as prostaglandins, platelet activating factor, and leukotrienes; for
peptide hormones such as
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calcitonin, CSa anaphylatoxin, follicle stimulating hormone, gonadotropin
releasing hormone, neurokinin,
oxytocin, and thrombin; and for sensory signal mediators such as retinal
photopigments and olfactory
stimulatory molecules. The structure of these highly conserved receptors
consists of seven
hydrophobic transmembrane regions, cysteine disulfide bridges between the
second and third
s extracellular loops, an extracellular N-terminus, and a cytoplasmic C-
terminus. The N-terminus
interacts with ligands, the disulfide bridges interact with agonists and
antagonists, and the large third
intracellular loop interacts with G proteins to activate second messengers
such as cyclic AMP,
phospholipase C, inositol triphosphate, or ion channels. (Reviewed in Watson,
S. and Arkinstall, S.
(1994) The G protein Linked Receptor Facts Book, Academic Press, San Diego CA,
pp. 2-6; and
to Bolander, F.F. (1994) Molecular Endocrinolo~y, Academic Press, San Diego
CA, pp. 162-176.)
Other types of receptors include cell. surface antigens identified on
leukocytic cells of the
immune system. These antigens have been identified using systematic,
monoclonal antibody (mAb)-
based "shot gun" techniques. These techniques have resulted in the production
of hundreds of mAbs
directed against unknown cell surface leukocytic antigens. These antigens have
been grouped into
15 "clusters of differentiation" based on common
immunocytochemical,localization patterns in various
differentiated and undifferentiated leukocytic cell types. Antigens in a given
cluster are presumed to
identify a single cell surface protein and are assigned a "cluster of
differentiation" or "CD"
designation. Some of the genes encoding proteins identified by CD antigens
have been cloned and
verified by standard molecular biology techniques. CD antigens have been
characterized as both
2 0 transmembrane proteins and cell surface proteins anchored to the plasma
membrane via covalent
attachment to fatty acid-containing.glycolipids such as
glycosylphosphatidylinositol (GPI). (Reviewed
in Barclay, A.N. et al. (1995) The Leucocvte Antigen Facts Book, Academic
Press, San Diego CA,
pP. 17-20.)
Matrix proteins (MPs) are transmembrane and extracellular proteins which
function in
2 s formation, growth, remodeling, and maintenance of tissues and as important
mediators and regulators
of the inflammatory response. The expression, and balance of MPs may be
perturbed by biochemical
changes that result from congenital, epigenetic, or infectious diseases. In
addition, MPs affect
leukocyte migration, proliferation, differentiation, and activation in the
immune response. MPs are
frequently characterized by the presence of one or more domains which may
include collagen-like
s o domains, EGF-like domains, immunoglobulin-like domains, and fibronectin-
like domains. In addition,
MPs may be heavily glycosylated and may contain an Arginine-Glycine-Aspartate
(RGD) tripeptide
motif which may play a role in adhesive interactions. MPs include
extracellular proteins such as
fibronectin, collagen, galectin, vitronectin and its proteolytic derivative
somatomedin B; and cell
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adhesion receptors such as cell adhesion molecules (CAMs), cadherins, and
integrins. (Reviewed in
Ayad, S. et al. (1994) The Extracellular Matrix Facts Book, Academic Press,
San Diego CA, pp. 2-
16; Ruoslahti, E. (1997) Kidney Int. 51:1413-1417; Sjaastad, M.D. and Nelson,
W.J. (1997) BioEssays
19:47-55.) '
s Cytokines are secreted by hematopoietic cells in response to injury or
infection. Interleukins,
neurotrophins, growth factors, interferons, and chemokines all define cytokine
families that work in
conjunction with cellular receptors to regulate cell proliferation and
differentiation. In addition,
cytokines effect activities such as leukocyte migration and function,
hematopoietic cell proliferation,
temperature regulation, acute response to infection, tissue remodeling, and
apoptosis.
to Chemokines, in particular, are small chemoattractant cytokines involved in
inflammation,
leukocyte proliferation and migration, angiogenesis and angiostasis,
regulation of hematopoiesis, HIV
infectivity, and stimulation of cytokine secretion. Chemokines generally
contain 70-100 amino acids
and are subdivided into four subfamilies based on the presence of conserved
cysteine-based motifs.
(Callard, R. and Gearing, A. (1994) The Cytokine Facts Book, Academic Press,
New York NY, pp.
is 181-190, 210-213, 223-227.)
Growth and differentiation factors are secreted proteins which function in
intercellular
communication. Some factors require oligomerization or association with MPs
for activity. Complex
interactions among these factors and their receptors trigger intracellular
signal transduction pathways
that stimulate or inhibit cell division, cell differentiation, cell signaling,
and cell motility. Most growth
~ o and differentiation factors. act on cells in their local environment
(paracrine signaling). There are three
broad classes of growth and differentiation factors. The first class includes
the large polypeptide
growth factors such as epidermal growth factor, fibroblast growth factor,
transforming growth factor,
insulin-like growth factor, and platelet-derived growth factor. The second
class includes the
hematopoietic growth factors such as the colony stimulating factors (CSFs).
Hematopoietic growth
25 factors stimulate the proliferation and differentiation of blood cells such
as B-lymphocytes, T-
lymphocytes, erythrocytes, platelets, eosinophils, basophils~ neutrophils,
macrophages, and their stem.
cell precursors. The third class includes small peptide factors such as
bombesin, vasopressin,
oxytocin, endothelin, transferrin, angiotensin II, vasoactive intestinal
peptide, and bradykinin which
function as hormones to regulate cellular functions other than proliferation.
3 o Growth and differentiation factors play critical roles in neoplastic
transformation of cells in
vitro and in tumor progression in vivo. Inappropriate expression of growth
factors by tumor cells may
contribute to vascularization and metastasis of tumors. During hematopoiesis,
growth factor
misregulation can result in anemias, leukemias, and lymphomas. Certain growth
factors such as
CA 02419943 2003-02-20
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interferon are cytotoxic to tumor cells both in vivo and in vitro. Moreover,
some growth factors and
growth factor receptors are related both structurally and functionally to
oncoproteins. In addition,
growth factors affect transcriptional regulation of both proto-oncogenes and
oncosuppressor genes.
(Reviewed in Pimentel, E. (1994) Handbook of Growth Factors, CRC Press, Ann
Arbor MI, pp. 1-9.)
s Proteolytic enzymes or proteases either activate or deactivate proteins by
hydrolyzing peptide
bonds. Proteases are found in the cytosol, in membrane-bound compartments, and
in the extracellular
space. The major families are the zinc, serine, cysteine, thiol, and carboxyl
proteases.
Ion channels, ion pumps, and transport proteins mediate the transport of
molecules across
cellular membranes. Transport can occur by a passive, concentration-dependent
mechanism or can
to be linked to an energy source such as ATP hydrolysis. Symporters and
antiporters transport ions and
small molecules such as amino acids, glucose, and drugs. Symporters transport
molecules and ions
unidirectionally, and antiporters transport molecules and ions
bidirectionally. Transporter superfamilies
include facilitative transporters and active ATP-binding cassette transporters
which are involved in
multiple-drug resistance and the targeting of antigenic peptides to MHC Class
I molecules. These
15 transporters bind to a specific ion or other molecule and undergo a
conformational change in order to
transfer the ion or molecule across the membrane. (Reviewed in Alberts, B. et
al. (1994) Molecular
Biolo~y of The Cell, Garland,Publishing, New York NY, pp. 523-546.)
Ion channels are foimed by transmembrane proteins which create a lined
passageway across
the membrane through which water and ions, such as Na+, K+, Ca2+, and Cf,
enter and exit the cell.
a o For example, chloride channels are involved in the regulation of the
membrane electric potential as . .
well as absorption and secretion of ions across the membrane. Chloride
channels also regulate the
internal pH of membrane-bound organelles.
Ion pumps are ATPases which actively maintain membrane gradients. Ion pumps
are
classified as P, V, or F according to their structure and function. All have
one or more binding sites
25 for ATP in their cytosolic domains. The P-class ion pumps include Ca2+
ATPase and Na+/K+ ATPase
and function in transporting H+, Na+, K+, and Ca2+ ions. P-class pumps consist
of two a and two (3
transmembrane subunits; The V- and F-class ion pumps have similar structures
but transport only H+.
F class H+ pumps mediate transport across the membranes of mitochondria and
chloroplasts, while V-
class H+ pumps regulate acidity inside lysosomes, endosomes, and plant
vacuoles.
s o A family of structurally related intrinsic membrane proteins known as
facilitative glucose
transporters catalyze the movement of glucose and other selected sugars across
the plasma
membrane. The proteins in this family contain a highly conserved, large
transmembrane domain
comprised of 12 a-helices, and several weakly conserved, cytoplasmic and
exoplasmic domains.
4
CA 02419943 2003-02-20
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(Pessin, J.E. and Bell, G.I. (1992) Annu. Rev. Physiol. 54:911-930.)
Amino acid transport is mediated by Na+ dependent amino acid transporters.
These
transporters are involved in gastrointestinal and renal uptake of dietary and
cellular amino acids and in
neuronal reuptake of neurotransmitters. Transport of cationic amino acids is
mediated by the system
s y+ family and the cationic amino acid transporter (CAT) family. Members of
the CAT family share a
high degree of sequence homology, and each contains 12-14 putative
transmembrane domains. (Ito,
K. and Groudine, M. (1997) J. Biol. Chem. 272:26780-26786.)
Hormones are secreted molecules that travel through the circulation and bind
to specific
receptors on the surface of, or within, target cells. Although they have
diverse biochemical
to compositions and mechanisms of action, hormones can be grouped into two
categories. One category
includes small lipophilic hormones that diffuse through the plasma membrane of
target cells, bind to
cytosolic or nuclear receptors, and form a complex that alters gene
expression. Examples of these
molecules include retinoic acid, thyroxine, and the cholesterol-derived
steroid hormones such as
progesterone, estrogen, testosterone, cortisol, and aldosterone. The second
category includes
is hydrophilic hormones that function by binding to cell surface receptors
that transduce signals across
the plasma membrane. Examples of such hormones include amino acid derivatives
such as
catecholamines and peptide hormones such as glucagon, insulin, gastrin,
secretin, cholecystokinin,
adrenocorticotropic hormone, follicle stimulating hormone, luteinizing
hormone, thyroid stimulating
hormone, and vasopressin. (See, for example, Lodish et al. (1995) Molecular
Cell Biology, Scientific,
2 o American Books Inc., New York NY, pp. 856-864.)
Neuropeptides and vasomediators (NP/VM) comprise a large family of endogenous
signaling
molecules. Included in this family are neuropeptides and neuropeptide hormones
such as bombesin,
neuropeptide Y, neurotensin, neuromedin N, melanocortins, opioids, galanin,
somatostatin, tachykinins,
urotensin II and related peptides involved in smooth muscle stimulation,
vasopressin, vasoactive
a s intestinal peptide, and circulatory system-borne signaling molecules such
as angiotensin, complement,
calcitonin, endothelins, formyl-methionyl peptides, glucagon, cholecystokinin
and gastrin. NP/VMs can
transduce signals directly, modulate the activity or release of other
neurotransmitters and hormones,
and act as catalytic enzymes in cascades. The effects of NP/VMs range from
extremely brief to
long-lasting. (Reviewed in Martin, C.R. et al. (1985) Endocrine Physioloey,
Oxford University Press,
3 o New York, NY, pp. 57-62.)
The discovery of new secretory molecules satisfies a need in the art by
providing new
compositions which are useful in the diagnosis, study, prevention, and
treatment of diseases associated
with, as well as effects of exogenous compounds on, cell signaling and the
expression of secretory
CA 02419943 2003-02-20
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molecules.
SUMMARY OF THE INVENTION
The present invention relates to nucleic acid sequences comprising human
polynucleotides
s encoding secretory polypeptides that contain signal peptides andlor
transmembrane domains. These
human polynucleotides (sptm) as presented in the Sequence Listing uniquely
identify partial or full
length genes encoding structural, functional, and regulatory polypeptides
involved in cell signaling.
The invention provides an isolated polynucleotide selected from the group
consisting of a) a
polynucleotide comprising a polynucleotide sequence selected from the group
consisting of SEQ ID
to N0:1-184; b) a polynucleotide comprising a naturally occurring
polynucleotide sequence at least 90%
identical to a polynucleotide sequence selected from the group consisting of
SEQ ID NO:1-184; c) a
polynucleotide complementary to the polynucleotide of a); d) a polynucleotide
complementary to the
polynucleotide of b); and e) an RNA equivalent of a) through d). In one
alternative, the polynucleotide
comprises a polynucleotide sequence selected from the group consisting of SEQ
1D NO:1-184. In
is another alternative, the polynucleotide comprises at least 30 contiguous
nucleotides of a polynucleotide
selected from the group consisting of a) a polynucleotide comprising a
polynucleotide sequence
selected from the group consisting of SEQ ID N0:1-184; b) a polynucleotide
comprising a naturally
occurring polynucleotide comprising a polynucleotide sequence at least 90%
identical to a
polynucleotide sequence'selected~from the group consisting of SEQ III NO:1-
184; c) a polyriucleotide .
2 o complementary to the polynucleotide of a); d) a polynucleotide
complementary to the polynucleotide of
b); and e) an RNA equivalent of a) through d). In another alternative, the
polynucleotide comprises at
least 60 contiguous nucleotides.of a polynucleotide selected from the group
consisting of a) a
polynucleotide comprising a polynucleotide sequence selected from the group
consisting of SEQ ID
NO:l-184; b) a polynucleotide comprising a naturally occurring polynucleotide
comprising a
25 polynucleotide sequence at least 90% identical to a polynucleotide sequence
selected from the group
consisting of SEQ ~ NO:1-184; c) a polynucleotide complementary to the
polynucleotide of a); d) a
polynucleotide complementary to the polynucleotide of b); and e) an RNA
equivalent of a) through d).
The invention further provides a composition for the detection of expression
of secretory
polynucleotides comprising at least one isolated polynucleotide comprising a
polynucleotide selected
s o from the group consisting of a) a polynucleotide comprising a
polynucleotide sequence selected from
the group consisting of SEQ ID N0:1-184; b) a polynucleotide comprising a
naturally occurring
polynucleotide sequence at least 90% identical to a polynucleotide sequence
selected from the group
consisting of SEQ ID NO:1-184; c) a polynucleotide complementary to the
polynucleotide of a); d) a
6
CA 02419943 2003-02-20
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polynucleotide complementary to the polynucleotide of b); and e) an RNA
equivalent of a) through d);
and a detectable label.
The invention also provides a method for detecting a target polynucleotide in
a sample, said
target polynucleotide having a polynucleotide sequence of a polyneucleotide
selected from the group
s consisting of a) a polynucleotide comprising a polynucleotide sequence of a
polynucleotide selected
from the group consisting of SEQ ID N0:1-184; b) a polynucleotide comprising a
naturally occurring
polynucleotide sequence at least 90% identical to a polynucleotide sequence
selected from the group
consisting of SEQ ID NO:1-184; c) a polynucleotide complementary to the
polynucleotide of a); d) a
polynucleotide complementary to the polynucleotide of b); and e) an RNA
equivalent of a) through d).
to The method comprises a) amplifying said target polynucleotide or fragment
thereof using polymerase
chain reaction amplification, and b) detecting the presence or absence of said
amplified target
polynucleotide or fragment thereof, and, optionally, if present, the amount
thereof.
The invention also provides a method for detecting a target polynucleotide in
a sample, said
target polynucleotide having a polynucleotide sequence of a polynucleotide
selected from the group
is consisting of a) a polynucleotide comprising a polynucleotide sequence
selected from the group
consisting of SEQ ID N0:1-184; b) a polynucleotide comprising a naturally
occurring polynucleotide
sequence at least 90% identical to a polynucleotide sequence selected from the
group consisting of
SEQ ID NO:1-184; c) a polynucleotide complementary to the polynucleotide of
a); d) a polynucleotide
complementary to the polynucleotide of b); and e) an RNA equivalent of a)
through d). The method.
2o comprises a) hybridizing the sample with a probe comprising at least 20
contiguous nucleotides
comprising a sequence complementary to said target polynucleotide in the
sample; and which probe
specifically hybridizes to said target polynucleotide, under conditions
whereby a hybridization complex
is formed between said probe and said target polynucleotide, and b) detecting
the presence or absence
of said hybridization complex, and, optionally, if present, the amount
thereof. In one alternative, the
2 s invention provides a composition comprising a target polynucleotide of the
method, wherein said probe
comprises at least 30 contiguous nucleotides. In one alternative, the
invention provides a composition
comprising a target polynucleotide of the method, wherein said probe comprises
at least 60 contiguous
nucleotides.
The invention further provides a recombinant polynucleotide comprising a
promoter sequence
3 0 operably linked to an isolated polynucleotide selected from the group
consisting of a) a polynucleotide
comprising a polynucleotide sequence selected from the group consisting of SEQ
ID N0:1-184; b) a
polynucleotide comprising a naturally occurring polynucleotide sequence at
least 90% identical to a
polynucleotide sequence selected from the group consisting of SEQ ID NO:1-184;
c) a polynucleotide
7
CA 02419943 2003-02-20
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complementary to the polynucleotide of a); d) a polynucleotide complementary
to the polynucleotide of
b); and e) an RNA equivalent of a) through d). In one alternative, the
invention provides a cell
transformed with the recombinant polynucleotide. In another alternative, the
invention provides a
transgenic organism comprising the recombinant polynucleotide.
The invention also provides a method for producing a secretory polypeptide,
the method
comprising a) culturing a cell under conditions suitable for expression of the
secretory polypeptide,
wherein said cell is transformed with a recombinant polynucleotide, said
recombinant polynucleotide
comprising an isolated polynucleotide selected from the group consisting of i)
a polynucleotide
comprising a polynucleotide sequence selected from the group consisting of SEQ
ID N0:1-184; ii) a
so polynucleotide comprising a naturally occurring polynucleotide sequence at
least 90% identical to a
polynucleotide sequence selected from the group consisting of SEQ ID NO:1-184;
iii) a polynucleotide
complementary to the polynucleotide of i); iv) a polynucleotide complementary
to the polynucleotide of
ii); and v) an RNA equivalent of i) through iv), and b) recovering the
secretory polypeptide so
expressed. The invention additionally provides a method wherein the
polypeptide has an amino acid ,
is sequence selected from the group consisting of SEQ ID N0:185-369.
The invention also provides an isolated secretory polypeptide (SPTM) encoded
by at least one
polynucleotide comprising a polynucleotide sequence selected from the group
consisting of SEQ ~ID
NO:1-184. The invention further provides a method of screening for a test
compound that specifically
binds to the polypeptide having an amino acid sequence selected from the group
consisting of SEQ ID
a o N0:185-369. The method comprises a) combining the polypeptide having an
amino acid sequence=,
selected from the group consisting of SEQ ID N0:185-369 with at least one test
compound under
suitable conditions, and b) detecting binding of the polypeptide having an
amino acid sequence selected
from the group consisting of.SEQ ID N0:185-369 to the test compound, thereby
identifying a
compound that specifically binds to the polypeptide having an amino acid
sequence selected from the
25 group consisting of SEQ ID N0:185-369.
The invention further provides a microarray wherein at least one element of
the microarray is
an isolated polynucleotide comprising at least 30 contiguous nucleotides of a
polynucleotide selected
from the group consisting of a) a polynucleotide comprising a polynucleotide
sequence selected from
the group consisting of SEQ ID N0:1-184; b) a polynucleotide comprising a
naturally occurnng
s o polynucleotide sequence at least 90% identical to a polynucleotide
sequence selected from the group
consisting of SEQ E3 N0:1-184; c) a polynucleotide complementary to the
polynucleotide of a); d) a
polynucleotide complementary to the polynucleotide of b); and e) an RNA
equivalent of a) through d).
The invention also provides a method for generating a transcript image of a
sample which contains
8
CA 02419943 2003-02-20
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polynucleotides. The method comprises a) labeling the polynucleotides of the
sample, b) contacting
the elements of the microarray with the labeled polynucleotides of the sample
under conditions suitable
for the formation of a hybridization complex, and c) quantifying the
expression of the polynucleotides
in the sample.
s Additionally, the invention provides a method for screening a compound for
effectiveness in
altering expression of a target polynucleotide, wherein said target
polynucleotide comprises a
polynucleotide selected from the group consisting of a) a polynucleotide
comprising a polynucleotide
sequence selected from the group consisting of SEQ ID N0:1-184; b) a
polynucleotide comprising a
naturally occurring polynucleotide sequence at least 90% identical to a
polynucleotide sequence
so selected from the group consisting of SEQ ID NO:1-184; c) a polynucleotide
complementary to the
polynucleotide of a); d) a polynucleotide complementary to the polynucleotide
of b); and e) an RNA
equivalent of a) through d). The method comprises a) exposing a sample
comprising the target
polynucleotide to a compound, b) detecting altered expression of the target
polynucleotide, and c)
comparing the expression of the target polynucleotide in the presence of
varying amounts of the
is compound and in the absence of the compound.
The invention further provides a method for assessing toxicity of a test
compound, said
method comprising a) treating a biological sample containing nucleic acids
with the test compound; b)
hybridizing the nucleic acids of the treated biological sample with a probe
comprising at least 20
contiguous nucleotides of a polynucleotide selected from the group consisting
of i) a polynucleotide
2 o comprising a polynucleotide sequence selected from the group consisting of
SEQ ID NO:1-184; ii) a
polynucleotide comprising a naturally occurring polynucleotide sequence at
least 90% identical to a
polynucleotide sequence selected from the group consisting of SEQ ff~ NO:1-
184; iii) a polynucleotide
complementary to the polynucleotide of i); iv) a polynucleotide complementary
to the polynucleotide
of ii); and v) an RNA equivalent of i) through iv). . Hybridization occurs
under conditions whereby a
25 specific hybridization complex is formed between said probe and a target
polynucleotide in the
biological sample, said target polynucleotide comprising a polynucleotide
sequence of a polynucleotide
selected from the group consisting of i) a polynucleotide comprising a
polynucleotide sequence
selected from the group consisting of SEQ )D NO:1-184; ii) a polynucleotide
comprising a naturally
occurring polynucleotide sequence at least 90% identical to a polynucleotide
sequence selected from
s o the group consisting of SEQ ID NO:1-184; iii) a polynucleotide
complementary to the polynucleotide of
i); iv) a polynucleotide complementary to the polynucleotide of ii); and v) an
RNA equivalent of i)
through iv), and alternatively, the target polynucleotide comprises a
polynucleotide sequence of a
fragment of a polynucleotide selected from the group consisting of i-v above;
c) quantifying the
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
amount of hybridization complex; and d) comparing the amount of hybridization
complex in the treated
biological sample with the amount of hybridization complex in an untreated
biological sample, wherein
a difference in the amount of hybridization complex in the treated biological
sample is indicative of
toxicity of the test compound.
The invention further provides an isolated polypeptide selected from the group
consisting of a)
a polypeptide comprising an amino acid sequence selected from the group
consisting of SEQ ID
N0:185-369, b) a polypeptide comprising a naturally occurring amino acid
sequence at least 90%
identical to an amino acid sequence selected from the group consisting of SEQ
ID NO:185-369, c) a
biologically active fragment of a polypeptide having an amino acid sequence
selected from the group
to consisting of SEQ ID N0:185-369, and d) an immunogenic fragment of a
polypeptide having an amino
acid sequence selected from.the group consisting of SEQ ID N0:185-369. In one
alternative, the
invention provides an isolated polypeptide comprising an amino acid sequence
selected from the group
consisting of SEQ 1D N0:185-369.
The invention further provides an isolated polynucleotide encoding a
polypeptide selected from
15 the group consisting of a) a polypeptide comprising an amino acid sequence
selected from the group
consisting of SEQ 1D N0:185-369, b) a polypeptide comprising a naturally
occurring amino acid
sequence at least 90% identical to an amino acid sequence selected from the
group consisting of SEQ.
ID N0:185-369, c) a biologically active fragment of a polypeptide having an
amino acid sequence
selected from the group consisting of SEQ ff~ N0:185-369, and d) an
immunogenic fragment of a
2 o polypeptide having an amino acid sequence selected from the group
consisting of SEQ ID N0:185-
369. In one alternative, the polynucleotide encodes a polypeptide comprising
an amino acid sequence
selected from the group consisting of SEQ ID N0:185-369. In another
alternative, the polynucleotide
comprises a polynucleotide sequence selected from the group consisting of SEQ
ID NO:1-184.
Additionally, the invention provides an isolated antibody which specifically
binds to a
2s polypeptide selected from the group consisting of a) apolypeptide
comprising an amino acid sequence
selected from the group consisting of SEQ ID NO:185-369, b) a polypeptide
comprising a naturally
occurring amino acid sequence at least 90% identical to an amino acid sequence
selected from the
group consisting of SEQ ID N0:185-369, c) a biologically active fragment of a
polypeptide having an
amino acid sequence selected from the group consisting of SEQ ID N0:185-369,
and d) an
s o immunogenic fragment of a polypeptide having an amino acid sequence
selected from the group
consisting of SEQ ID N0:185-369.
The invention further provides a composition comprising a polypeptide selected
from the group
consisting of a) a polypeptide comprising an amino acid sequence selected from
the group consisting
to
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
of SEQ ID N0:185-369, b) a polypeptide comprising a naturally occurring amino
acid sequence at
least 90% identical to an amino acid sequence selected from the group
consisting of SEQ 1D N0:185-
369, c) a biologically active fragment of a polypeptide having an amino acid
sequence selected from
the group consisting of SEQ 1D N0:185-369, and d) an immunogenic fragment of a
polypeptide having
s an amino acid sequence selected from the group consisting of SEQ ID NO 185-
369, and a
pharmaceutically acceptable excipient. In one embodiment, the composition
comprises a polypeptide
having an amino acid sequence selected from the group consisting of SEQ ID
N0:185-369. The
invention additionally provides a method of treating a disease or condition
associated with decreased
expression of functional SPTM, comprising administering to a patient in need
of such treatment the
to composition.
The invention also provides a method for screening a compound for
effectiveness as an
agonist of a polypeptide selected from the group consisting of a) a
polypeptide comprising an amino
acid sequence selected from the group consisting of SEQ ID N0:185-369, b) a
polypeptide comprising
a naturally occurring amino acid sequence at least ~90% identical to an amino
acid sequence selected
15 from the group consisting of SEQ ID N0:185-369, c) a biologically active
fragment of a polypeptide
having an amino acid sequence selected from the group consisting of SEQ ID
N0:185;369~ and d) an
immunogenic fragment of a polypeptide having an amino acid sequence selected
from the group
consisting of SEQ ID NO:185-369. The method comprises a) exposing a sample
comprising the
polypeptide to a compound, and b) detecting agonist activity in the sample. In
one alternative, the
2 o invention provides a composition comprising an agonist compound identified
by the method and a
pharmaceutically acceptable excipient. In another alternative, the invention
provides a method of
treating a disease or condition associated with 'decreased expression of
functional SPTM, comprising
administering to a patient in need of such treatment the composition.
Additionally, the invention provides a method for screening a compound for
effectiveness as
a s an antagonist of a polypeptide selected from the group consisting of a) a
polypeptide comprising an
amino acid sequence selected from the group consisting of SEQ ID N0:185-369 b)
a polypeptide
comprising a naturally occurring amino acid sequence at least 90% identical to
an amino acid
sequence selected from the group consisting of SEQ )D N0:185-369, c) a
biologically active fragment
of a polypeptide having an amino acid sequence selected from the group
consisting of SEQ ID
s o NO:l 85-369, and d) an immunogenic fragment of a polypeptide having an
amino acid sequence
selected from the group consisting of SEQ 11~ N0:185-369. The method comprises
a) exposing a
sample comprising the polypeptide to a compound, and b) detecting antagonist
activity in the sample.
In one alternative, the invention provides a composition comprising an
antagonist compound identified
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by the method and a pharmaceutically acceptable excipient. In another
alternative, the invention
provides a method of treating a disease or condition associated with
overexpression of functional
SPTM, comprising administering to a patient in need of such treatment the
composition.
The invention further provides a method of screening for a compound that
modulates the
s activity of a polypeptide selected from the group consisting of a) a
polypeptide comprising an amino
acid sequence selected from the group consisting of SEQ ID N0:185-369, b) a
polypeptide comprising
a naturally occurring amino acid sequence at least 90% identical to an amino
acid sequence selected
from the group consisting of SEQ ID NO:185-369, c) a biologically active
fragment of a polypeptide
having an amino acid sequence selected from the group consisting of SEQ ID NO
185-369, and d) an
to immunogenic fragment of a polypeptide having an amino acid sequence
selected from the group
consisting of SEQ ID N0:185-369. The method comprises a) combining the
polypeptide with at least
one test compound under conditions permissive for the activity of the
polypeptide, b) assessing the
activity of the polypeptide in the presence of the test compound, and c)
comparing the activity of the
polypeptide in the presence of the test compound with the activity of the
polypeptide in the absence of
15 the test compound, wherein a change in the activity of the polypeptide in
the presence of the test
compound is indicative of a compound that modulates the activity of the
polypeptide.
DESCRIPTION OF THE 'TABLES
Table 1 shows the sequence identification numbers (SEQ ID NOa) and template
identification
2 o numbers (template mss) corresponding to the polynucleotides of the present
invention, along with they.
sequence identification numbers (SEQ ID NOa) and open reading frame
identification numbers (ORF
IDs) corresponding to polypeptides encoded by the template ID.
Table 2 shows the sequence identification numbers (SEQ ID NOa) and template
identification
numbers (template IDs) corresponding to the polynucleotides of the present
invention, along with
2 s polynucleotide segments of each template sequence as defined by the
indicated "start" and "stop"
nucleotide positions. The reading frames of the polynucleotide segments are
shown, and the
polypeptides encoded by the polynucleotide segments constitute either signal
peptide (SP) or
transmembrane (TM) domains, as indicated. The membrane topology of the encoded
polypeptide
sequence is indicated, the N-terminus (N) listed as being oriented to either
the cytosolic (N in) or non-
s o cytosolic (N out) side of the cell membrane or organelle.
Table 3 shows the sequence identification numbers (SEQ ID NOa) and template
identification
numbers (template IDs) corresponding to the polynucleotides of the present
invention, along with
component sequence identification numbers (component IDs) corresponding to
each template. The
12
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component sequences, which were used to assemble the template sequences, are
defined by the
indicated "start" and "stop" nucleotide positions along each template.
Table 4 shows the tissue distribution profiles for the templates of the
invention.
Table 5 shows the sequence identification numbers (SEQ ID NOa) corresponding
to the
s polypeptides of the present invention, along with the reading frames used to
obtain the polypeptide
segments, the lengths of the polypeptide segments, the "start" and "stop"
nucleotide positions of the
polynucleotide sequences used to define the encoded polypeptide segments, the
GenBank hits (GI
Numbers), probability scores, and functional annotations corresponding to the
GenBank hits.
Table 6 summarizes the bioinformatics tools which are useful for analysis of
the
so polynucleotides of the present invention. The first column of Table 6 lists
analytical tools, programs,
and algorithms, the second column provides brief descriptions thereof, the
third column presents
appropriate references, all of which are incorporated by reference herein in
their entirety, and the
fourth column presents, where applicable, the scores, probability values, and
other parameters used to
evaluate the strength of a match between two sequences (the higher the score,
the greater the
is homology between two sequences).
DETAILED DESCRIPTION OF THE INVENTION
Before the nucleic acid sequences and methods are presented, it is to be
understood that this
invention is not limited to the particular machines, methods, and materials
described. Although
2 o particular embodiments are described, machines, methods, and materials
similar or equivalent to these
embodiments may be used to practice the invention. The preferred machines,
methods, and materials
set forth are not intended to limit the scope of the invention which is
limited only by the appended
claims.
The singular forms "a", "an", and "the" include plural reference unless the
context clearly
25 dictates otherwise. All technical and scientific terms have the meanings
commonly understood by one
of ordinary skill in the art. All publications are incorporated by reference
for the purpose of describing
and disclosing the cell lines, vectors, and methodologies which are presented
and which might be used
in connection with the invention. Nothing in the specification is to be
construed as an admission that
the invention is not entitled to antedate such disclosure by virtue of prior
invention.
Definitions
As used herein, the lower case "sptm" refers to a nucleic acid sequence, while
the upper case
"SPTM" refers to an amino acid sequence encoded by sptm. A "full-length" sptm
refers to a nucleic
13
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acid sequence containing the entire coding region of a gene endogenously
expressed in human tissue.
"Adjuvants" are materials such as Freund's adjuvant, mineral gels (aluminum
hydroxide), and
surface active substances (lysolecithin, pluronic polyols, polyanions,
peptides, oil emulsions, keyhole
limpet hemocyanin, and dinitrophenol) which may be administered to increase a
host's immunological
s ~ response.
"Allele" refers to an alternative form of a nucleic acid sequence. Alleles
result from a
"mutation," a change or an alternative reading of the genetic code. Any given
gene may have none,
one, or many allelic forms. Mutations which give rise to alleles include
deletions, additions, or
substitutions of nucleotides. Each of these changes may occur alone, or in
combination with the
so others, one or more times in a given nucleic acid sequence. The present
invention encompasses allelic
sptm
"Amino acid sequence" refers to a peptide, a polypeptide, or a protein of
either natural or
synthetic origin. The amino acid sequence is not limited to the complete,
endogenous amino acid
sequence and may be a fragment, epitope, variant, or derivative of a protein
expressed by a nucleic
is acid sequence.
"Amplification" refers to the production of additional copies of a sequence
and is earned out.
using polymerase chain reaction (PCR) technologies well known in the art.
"Antibody" refers to intact molecules as well as to fragments thereof, such as
Fab, F(ab')2,
and Fv fragments, which are capable of binding the epitopic determinant.
Antibodies that bind SPTM
2 o polypeptides can be prepared using intact polypeptides or using fragments
containing small peptides of
interest as the immunizing antigen. The polypeptide or peptide used to
immunize an animal (e:g., a
mouse, a rat, or a rabbit) can be derived from the translation of RNA, or
synthesized chemically, and
can be conjugated to a'carrier protein if desired. Commonly used carriers that
are chemically coupled
to peptides include bovine serum albumin, thyroglobulin, and keyhole limpet
hemocyanin (KLI~. The
2 s coupled peptide is then used to immunize the animal.
"Antisense sequence" refers to a sequence capable of specifically hybridizing
to a target
sequence. ,The antisense sequence may include DNA, RNA, or any nucleic acid
mimic or analog
such as peptide nucleic acid (PNA); oligonucleotides having modified backbone
linkages such as
phosphorothioates, methylphosphonates, or benzylphosphonates; oligonucleotides
having modified
s o sugar groups such as 2'-methoxyethyl sugars or 2'-methoxyethoxy sugars; or
oligonucleotides having
modified bases such as 5-methyl cytosine, 2'-deoxyuracil, or 7-deaza-2'-
deoxyguanosine.
"Antisense sequence" refers to a sequence capable of specifically hybridizing
to a target
sequence. The antisense sequence can be DNA, RNA, or any nucleic acid mimic or
analog.
14
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l
"Antisense technology" refers to any technology which relies on the specific
hybridization of
an antisense sequence to a target sequence.
A "bin" is a portion of computer memory space used by a computer program for
storage of
data, and bounded in such a manner that data stored in a bin may be retrieved
by the program.
s "Biologically active" refers to an amino acid sequence having a structural,
regulatory, or
biochemical function of a naturally occurring amino acid sequence.
"Clone joining" is a process for combining gene bins based upon the bins'
containing sequence
information from the same clone. The sequences may assemble into a primary
gene transcript as well
as one or more splice variants.
to "Complementary" describes the relationship between two single-stranded
nucleic acid
sequences that anneal by base-pairing (5'-A-G-T-3' pairs with its complement
3'-T-C-A-5').
A "component sequence" is a nucleic acid sequence selected by a computer
program such as
PHRED and used to assemble a consensus or template sequence from one or more
component
sequences.
15 A "consensus sequence" or "template sequence" is a nucleic acid sequence
which has been
. assembled from overlapping sequences, using a computer program for fragment
assembly such as the
GELVIEW fragment assembly system (Genetics Computer Group (GCG), Madison WI)
or using a
relational database rrianagement system (RDMS).
"Conservative amino acid substitutions" are those substitutions that, when
made, least
a o .interfere with the properties of the original protein, i.e., the
structure and especially the function of the
protein is conserved and not significantly changed by such substitutions. The
table below shows amino
acids which may be substituted for an original amino acid in a protein and
which are regarded as
conservative substitutions.
25 Original Residue Conservative Substitution
Ala Gly, Ser
Arg His, Lys
Asn Asp, Gln, His
Asp Asn, Glu
3 o Cys Ala, Ser
Gln Asn, Glu, His
Glu Asp, Gln, His
Gly Ala '
His Asn, Arg, Gln, Glu
3 5 Ile Leu, Val
Leu Ile, Val
Lys Arg, Gln, Glu
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Met Leu, Ile
Phe His, Met, Leu, Trp, Tyr
Ser Cys, Thr
'Thr Ser, Val
s Trp Phe, Tyr
Tyr His, Phe, Trp
Val Ile, L,eu, Thr
to Conservative substitutions generally maintain (a) the structure of the
polypeptide backbone in
the area of the substitution, for example, as a beta sheet or alpha helical
conformation, (b) the charge
or hydrophobicity of the molecule at the target site, or (c) the bulk of the
side chain.
"Deletion" refers to a change in either a nucleic or amino acid sequence in
which at least one
nucleotide or amino acid residue, respectively, is absent.
is "Derivative" refers to the chemical modification of a nucleic acid
sequence, such as by
replacement of hydrogen by an alkyl, acyl, amino, hydroXyl, or other group.
"Differential expression" refers to increased or upregulated; or decreased,
downregulated, or
absent gene ox protein expression, determined by comparing at least two
different samples. Such
comparisons may be carried out between, for example, a treated and an
untreated sample, or a
2 o diseased and a normal sample.
The terms "element" and "array element" refer to a polynucleotide,
polypeptide, or other
chemical compound having a unique and defined position on a microarray.
"E-value" refers to the statistical probability that a match between two
sequences occurred by.
chance.
25 "Exon shuffling" refers to the recombination of different coding regions
(exons). Since an
exon may represent a structural or functional domain of the encoded protein,
new proteins may be
assembled through the novel reassortment of stable substructures, thus
allowing acceleration of the
evolution of new protein functions.
A "fragment" is a unique portion of sptm or SPTM which is identical in
sequence to but
3 o shorter in length than the parent sequence. A fragment may comprise up to
the entire length of the
defined sequence, minus one nucleotide/amino acid residue. For example, a
fragment may comprise
from 10 to 1000 contiguous amino acid residues or nucleotides. A fragment used
as a probe, primer,
antigen, therapeutic molecule, or for other purposes, may be at least 5, 10,
15, 16, 20, 25, 30, 40, 50, 60,
75, 100, 150, 250 or at least S00 contiguous amino acid residues or
nucleotides in length. Fragments
35 may be preferentially selected from certain regions of a molecule. For
example, a polypeptide
fragment may comprise a certain length of.contiguous amino acids selected from
the first 250 or 500
16
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amino acids (or first 25% or 50%) of a polypeptide as shown in a certain
defined sequence. Clearly
these lengths are exemplary, and any length that is supported by the
specification, including the
Sequence Listing and the figures, may be encompassed by the present
embodiments.
A fragment of sptm comprises a region of unique polynucleotide sequence that
specifically
s identifies sptm, for example, as distinct from any other sequence in the
same genome. A fragment of
sptm is useful, for example, in hybridization and amplification technologies
and in analogous methods
that distinguish sptm from related polynucleotide sequences. The precise
length of a fragment of sptm
and the region of sptm to which the fragment corresponds are routinely
determinable by one of
ordinary skill in the ait based on the intended purpose for the fragment.
to A fragment of SPTM is encoded by a fragment of sptm. A fragment of SPTM
comprises a
region of unique amino acid sequence that specifically identifies SPTM. For
example, a fragment of
SPTM is useful as an immunogenic peptide for the development of antibodies
that specifically
recognize SPTM. The precise length of a fragment of SPTM and the region of
SPTM to which the
fragment corresponds are routinely determinable by one of ordinary skill in
the art based on the
is intended purpose for the fragment.
A "full length" nucleotide sequence is one containing at least a start site
for translation to a
protein sequence, followed liy an open reading frame and a stop site, and
encoding a "full length"
polypeptide.
"Hit" refers to a sequence whose annotation will be used to describe a given
template.
~ o Criteria for selecting the top hit are as follows: if the template has one
or more exact nucleic acid
matches, the top hit is the exact match with highest percent identity. If the
template has no exact
matches but has significant protein hits, the top hit is the protein hit with
the lowest E-value. If the
template has no significant protein hits, but does have significant non-exact
nucleotide hits, the top hit
is the nucleotide hit with the lowest E-value.
2 s "Homology" refers to sequence similarity either between a reference
nucleic acid sequence
and at least a fragment of an sptm or between a reference amino acid sequence
and a fragment of an
SPTM.
"Hybridization" refers to the process by which a strand of nucleotides anneals
with a
complementary strand through base pairing. Specific hybridization is an
indication that two nucleic
s o acid sequences share a high degree of identity. Specific hybridization
complexes form under defined
annealing conditions, and remain hybridized after the "washing" step. The
defined hybridization
conditions include the annealing conditions and the washing step(s), the
latter of which is particularly
important in determining the stringency of the hybridization process, with
more stringent conditions
17
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allowing less non-specific binding, i.e., binding between pairs of nucleic
acid probes that are not
perfectly matched. Permissive conditions for annealing of nucleic acid
sequences are routinely
determinable and may be consistent among hybridization experiments, whereas
wash conditions may
be varied among experiments to achieve the desired stringency.
s Generally, stringency of hybridization is expressed with reference to the
temperature under
which the wash step is carried out. Generally, such wash temperatures are
selected to be about 5°C
to 20°C lower than the thermal melting point (T"~ for the specific
sequence at a defined ionic strength
and pH. The Tm is the temperature (under defined ionic strength and pH) at
which 50% of the target
sequence hybridizes to a perfectly matched probe. An equation for calculating
Tm and conditions for
to nucleic acid hybridization is well known and can be found in Sambrook et
al., 1989, Molecular Cloning:
A Laborator~Manual, 2°d ed., vol. 1-3, Cold Spring Harbor Press,
Plainview NY; specifically see
volume 2, chapter 9.
High stringency conditions for hybridization between polynucleotides of the
present invention
include wash conditions of 68°C in the presence of about 0.2 x SSC and
about 0.1 % SDS, for 1. hour.
15 Alternatively, temperatures of about 65°C, 60°C, or
55°C may be used. SSC concentration may be
varied from about 0.2 to 2 x SSC, with SDS being present at about 0.1 %.
Typically, blocking reagents
are used to block non-specific hybridization. Such blocking reagents include,
for instance, denatured
salmon sperm DNA at about 100-200 ~g/ml. Useful variations on these conditions
will be readily .
apparent to those skilled in the art. Hybridization, particularly under high
stringency conditions, may be
2 o suggestive of evolutionary similarity between the nucleotides. Such
similarity is strongly indicative of a
similar role for the nucleotides and their resultant proteins:
Other parameters, such as temperature, salt concentration, and detergent
concentration may
be varied to achieve the desired stringency. Denaturants, such as formamide at
a concentration of
about 35-50% v/v, may also be used under particular circumstances, such as
RNA:DNA
2s hybridizations. Appropriate hybridization conditions are routinely
determinable by one of ordinary skill
in the art.
"Immunologically active" or "immunogenic" describes the potential for a
natural, recombinant,
or synthetic peptide, epitope, polypeptide, or protein to induce antibody
production in appropriate
animals, cells, or cell lines.
3 0 "Insertion" or "addition" refers to a change in either a nucleic or amino
acid sequence in
which at least one nucleotide or residue, respectively, is added to the
sequence.
"Labeling" refers to the covalent or noncovalent joining of a polynucleotide,
polypeptide, or
antibody with a reporter molecule capable of producing a detectable or
measurable signal.
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"Microarray" is any arrangement of nucleic acids, amino acids, antibodies,
etc., on a
substrate. The substrate may be a solid support such as beads, glass, paper,
nitrocellulose, nylon, or an
appropriate membrane.
"Linkers" are short stretches of nucleotide sequence which may be added to a
vector or an
s sptm to create restriction endonuclease sites to facilitate cloning.
"Polylinkers" are engineered to
incorporate multiple restriction enzyme sites and to provide for the use of
enzymes which leave 5' or 3'
overhangs (e.g., BamHI, EcoRI, and HindlTl) and those which provide blunt ends
(e.g., EcoRV,
SnaBI, and Stul).
"Naturally occurring" refers to an endogenous polynucleotide or polypeptide
that may be
to , isolated from viruses or prokaryotic or eukaryotic cells.
"Nucleic acid sequence" refers to the specific order of nucleotides joined by
phosphodiester
bonds in a linear, polymeric arrangement. Depending on the number of
nucleotides, the nucleic acid
sequence can be considered an oligomer, oligonucleotide, or polynucleotide.
The nucleic acid can be
DNA, RNA, or any nucleic acid analog, such as PNA, may be of genomic or
synthetic origin, may be
is either double-stranded or single-stranded, and can represent either the
sense or antisense
(complementary) strand.
"Oligomer" refers to a nucleic acid sequence of at least about 6 nucleotides
and as many as
about 60 nucleotides, preferably about 15 to 40 nucleotides, and most
preferably between about 20 and
30, nucleotides, that may be used in hybridization or amplification
technologies. Oligomers may be
2 o used as, e.g., primers for PCR, and are usually chemically synthesized.
"Operably linked" refers to the situation in which a first nucleic acid
sequence is placed in a
functional relationship with the second nucleic acid sequence. For instance, a
promoter is operably
linked to a coding sequence if the promoter affects the transcription or
expression of the coding
sequence. Generally, operably linked DNA sequences may be in close proximity
or contiguous and,
2 s where necessary to join two protein coding regions, in the same reading
frame.
"Peptide nucleic acid" (PNA) refers to a DNA mimic in which nucleotide bases
are attached
to a pseudopeptide backbone to increase stability. PNAs, also designated
antigene agents, can
prevent gene expression by targeting complementary messenger RNA.
The phrases "percent identity" and "% identity", as applied to polynucleotide
sequences, refer
3 o to the percentage of residue matches between at least two polynucleotide
sequences aligned using a
standardized algorithm. Such an algorithm may insert, in a standardized and
reproducible way, gaps in
the sequences being compared in order to optimize alignment between two
sequences, and therefore
achieve a more meaningful comparison of the two sequences.
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Percent identity between polynucleotide sequences may be determined using the
default
parameters of the CLUSTAL V algorithm as incorporated into the MEGALIGN
version 3.12e
sequence alignment program. This program is part of the LASERGENE software
package, a suite of
molecular biological analysis programs (DNASTAR, Madison WI). CLUSTAL V is
described in
s Higgins, D.G. and Sharp, P.M. '(1989) CABIOS 5:151-153 and in Higgins, D.G.
et al. (1992) CABIOS
8:189-191. For pairwise alignments of polynucleotide sequences, the default
parameters are set as
follows: Ktuple=2, gap penalty=5, window=4, and "diagonals saved"=4. The
"weighted" residue
weight table is selected as the default. Percent identity is reported by
CLUSTAL V as the "percent
similarity" between aligned polynucleotide sequence pairs.
to Alternatively, a suite of commonly used and freely available sequence
comparison algorithms
is provided by the National Center for Biotechnology Information (NCBI) Basic
Local Alignment
Search Tool (BLAST) (Altschul, S.F. et al. (1990) J. Mol. Biol. 215:403-410),
which is available from
several sources, including the NCBI, Bethesda, MD, and on the Internet at
http://www.ncbi.nlm.nih.govBLAST/. The BLAST software suite includes various
sequence analysis
15 programs including "blastn," that is used to determine alignment between a
known polynucleotide
sequence and other sequences on a variety of databases. Also available is a
tool called "BLAST 2
Sequences" that is used for direct pairwise comparison of two nucleotide
sequences. "BLAST 2 .
Sequences" can be accessed and.used interactively at
http:l/www.ncbi.nlm.nih.gov/gorf/b12/. The
"BLAST 2 Sequences" tool can be used for both blastn and blastp (discussed
below). BLAST
2 o programs are commonly used with gap and other parameters set to default
settings. For example, to
compare two nucleotide sequences, one may use blastn with the "BLAST 2
Sequences'' tool Version
2Ø9 (May-07-1999) set at default parameters. Such default parameters may be,
for example:
Matrix: BLOSUM62
Reward for match: 1
2 s Penalty for mismatch: -2,
Opera Gap: S afad Extensiota Gap: 2 penalties
Gap x drop-off 50
Expect: 10
Word Size: 11
3 o Filter: on
Percent identity may be measured over the length of an entire defined
sequence, for example,
as defined by a particular SEQ ID number, or may be measured over a shorter
length, for example,
over the length of a fragment taken from a larger, defined sequence, for
instance, a fragment of at
CA 02419943 2003-02-20
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least 20, at least 30, at least 40, at least 50, at least 70, at least 100, or
at least 200 contiguous
nucleotides. Such lengths are exemplary only, and it is understood that any
fragment lengthsupported
by the sequences shown herein, in figures or Sequence Listings, may be used to
describe a length over
which percentage identity may be measured.
s Nucleic acid sequences that do not show a high degree of identity may
nevertheless encode
similar amino acid sequences due to the degeneracy of the genetic code. It is
understood that changes
in nucleic acid sequence can be made using this degeneracy to produce multiple
nucleic acid
sequences that all encode substantially the same protein.
The phrases "percent identity" and "% identity", as applied to polypeptide
sequences, refer to
to the percentage of residue matches between at least two polypeptide
sequences aligned using a
standardized algorithm. Methods of polypeptide sequence alignment are well-
known. Some alignment
methods take into account conservative amino acid substitutions. Such
conservative substitutions,
explained in more detail above, generally preserve the hydrophobicity and
acidity of the substituted
residue, thus preserving the structure (and therefore function) of the folded
polypeptide.
15 Percent identity between polypeptide sequences may be determined using the
default
parameters of the CLUSTAL V algorithm as incorporated into the MEGALIGN
version 3.12e
sequence alignment program (described and referenced above). For pairwise
alignments of ..
polypeptide sequences using CLUSTAL V, the default parameters are set as
follows: Ktuple=1, gap
penalty=3, window=5, and "diagonals saved"=5. The PAM250 matrix is selecfed as
the default
2 o residue weight table. As with polynucleotide alignments, the percent
identity is reported by
CLUSTAL V as the "percent similarity" between aligned polypeptide sequence
pairs.
Alternatively the NCBI BLAST software suite may be used. For example, for a
pairwise
comparison of two polypeptide sequences, one may use the "BLAST 2 Sequences''
tool Version 2Ø9
(May-07-1999) with blastp set at default parameters. Such default parameters
may be, for example:
25 Matrix: BLOSUM62
Open Gap: 11 arad Exte~asion Gap: 1 penalty
Gap x drop-off 50
Expect: l0
Word Size: 3
s o Filter: on
Percent identity may be measured over the length of an entire defined
polypeptide sequence,
for example, as defined by a particular SEQ 117 number, or may be measured
over a shorter length,
for example, over the length of a fragment taken from a larger, defined
polypeptide sequence, for
21
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instance, a fragment of at least 15, at least 20, at least 30, at least 40, at
least 50, at least 70 or at least
150 contiguous residues. Such lengths are exemplary only, and it is understood
that any fragment
length supported by the sequences shown herein, in figures or Sequence
Listings, may be used to
describe a length over which percentage identity may be measured.
"Post-translational modification" of an SPTM may involve lipidation,
glycosylation,
phosphorylation, acetylation, racemization, proteolytic cleavage, and other
modifications known in the
art. These processes may occur synthetically or biochemically. Biochemical
modifications will vary
by cell type depending on the enzymatic milieu and the SPTM.
"Probe" refers to sptm or fragments thereof, which are used to detect
identical, allelic or
to related nucleic acid sequences. Probes are isolated oligonucleotides or
polynucleotides attached to a
detectable label or reporter molecule. Typical labels include radioactive
isotopes, ligands,
chemiluminescent agents, and enzymes. "Primers" are short nucleic acids,
usually DNA
oligonucleotides, which may be annealed to a target polynucleotide by
complementary base-pairing.
The primer may then be extended along the target DNA strand by a DNA
polymerise enzyme.
15 Primer pairs can be used for amplification (and identification) of a
nucleic acid sequence, e.g., by the
polymerise chain reaction (PCR).
Probes and primers as used in the present invention typically comprise at
least 15 contiguous
nucleotides of a known sequence. In order to enhance specificity, longer
probes and primers may also
be employed, such as probes and primers that comprise ~at least 20, 30, 40,
50, 60, 70, 80, 90, 100, or at
z o least 150 consecutive nucleotides of the disclosed nucleic acid sequences.
Probes and primers may be
considerably longer than these examples, and it is understood that any length
supposed by the
specification, including the figures and Sequence Listing, may be.used.
Methods for preparing and using probes and primers are described in the
references, for
example Sambrook et al., 1989, Molecular Clanin~: A Laboratory Manual,
2°d ed., vol. 1-3, Cold
a5 Spring Harbor Press, Plainview NY; Ausubel et a1.,1987, Current Protocols
in Molecular Biolo~y,
Greene Publ. Assoc. & Wiley-Intersciences, New York NY; Innis et al., 1990,
PCR Protocols, A
Guide to Methods and Applications, Academic Press, San Diego CA. PCR primer
pairs can be
derived from a known sequence, for example, by using computer programs
intended for that purpose
such as Primer (Version 0.5, 1991, Whitehead Institute for Biomedical
Research, Cambridge MA).
s o Oligonucleotides for use as primers are selected using software known in
the art for such
purpose. For example, OLIGO 4.06 software is useful for the selection of PCR
primer pairs of up to
100 nucleotides each, and for the analysis of oligonucleotides and larger
polynucleotides of up to 5,000
nucleotides from an input polynucleotide sequence of up to 32 kilobases.
Similar primer selection
22
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WO 02/20756 PCT/USO1/27297
programs have incorporated additional features for expanded capabilities. For
example, the PrimOU
primer selection program (available to the public from the Genome Center at
University of Texas
South West Medical Center, Dallas TX) is capable of choosing specific primers
from megabase
sequences and is thus useful for designing primers on a genome-wide scope. The
Primer3 primer
s selection program (available to the public from the Whitehead Institute/MIT
Center for Genome
Research, Cambridge MA) allows the user to input a "mispriming library," in
which sequences to
avoid as primer binding sites are user-specified. Primer3 is useful, in
particular, for the selection of
oligonucleotides for microarrays. (The source code for the latter two primer
selection programs may
also be obtained from their respective sources and modified to meet the user's
specific needs.) The
to PrimeGen program (available to the public from the UK Human Genome Mapping
Project Resource
Centre, Cambridge UK) designs primers based on multiple sequence alignments,
thereby allowing
selection of primers that hybridize to either the most conserved or least
conserved regions of aligned
nucleic acid sequences. Hence, this program is useful for identification of
both unique and conserved
oligonucleotides and polynucleotide fragments. The oligonucleotides and
polynucleotide fragments
15 , identified by any of the above selection methods are useful in
hybridization technologies, for example,
as PCR or sequencing primers, microarray elements, or specific probes to
identify fully or partially
complementary polynucleotides in a sample of nucleic acids. Methods of
oligonucleotide selection are
not limited to those described above.
"Purified" refers to molecules, either polynucleotides or polypeptides that
are isolated or
2o separated from their natural environment and are at least 60% free,
preferably at least 75% free, and ,
most preferably at least 90% free from other compounds with which they are
naturally associated.
A "recombinant nucleic acid" is a sequence that is not naturally occurring or
has a sequence
that is made by an artificial combination of two or more otherwise separated
segments of sequence.
This artificial combination is often accomplished by chemical synthesis or,
more commonly, by the
25 artificial manipulation of isolated segments of nucleic acids, e.g., by
genetic engineering techniques
such as those described in Sambrook, su ra. The term recombinant includes
nucleic acids that have
been altered solely by addition, substitution, or deletion of a portion of the
nucleic acid. Frequently, a
recombinant nucleic acid may include a nucleic acid sequence operably linked
to a promoter sequence.
Such a recombinant~nucleic acid may be part of a vector that is used, for
example, to transform a cell.
3 o Alternatively, such recombinant nucleic acids may be part of a viral
vector, e.g., based on a
vaccinia virus, that could be use to vaccinatea mammal wherein the recombinant
nucleic acid is
expressed, inducing a protective immunological, response in the mammal.
23
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"Regulatory element" refers to a nucleic acid sequence from nontranslated
regions of a gene,
and includes enhancers, promoters, introns, and 3' untranslated regions, which
interact with host
proteins to carry out or regulate transcription or translation.
"Reporter" molecules are chemical or biochemical moieties used for labeling a
nucleic acid, an
amino acid, or an antibody. They include radionuclides; enzymes; fluorescent,
chemiluminescent, or
chromogenic agents; substrates; cofactors; inhibitors; magnetic particles; and
other moieties known in
the art.
An "RNA equivalent," in reference to a DNA sequence, is composed of the same
linear
sequence of nucleotides as the reference DNA sequence with the exception that
all occurrences of
to the nitrogenous base thymine are replaced with uracil, and the sugar
backbone is composed of ribose
instead of deoxyribose.
"Sample" is used in its broadest sense. Samples may contain nucleic or amino
acids,
antibodies, or other materials, and may be derived from any source (e.g.,
bodily fluids including, but not
limited to, saliva, blood, and urine; chromosome(s), organelles, or membranes
isolated from a cell;
is genomic DNA, RNA, or cDNA in solution or bound to a substrate; and cleared
cells or tissues or blots
or imprints from such cells or tissues).
"Specific binding" or "specifically binding" refers to the interaction between
a protein or
peptide and its agonist, antibody, antagonist, or other binding partner. The
interaction is dependent
upon the presence of a particular structure of the protein, e.g., the
antigenic determinant or epitope,
2 o recognized by the binding molecule. For example, if an antibody is
specific for epitope "A," the
presence of a polypeptide containing epitope A, or the presence of free
unlabeled A, in a reaction
containing free labeled A and the antibody will reduce the amount of labeled A
that binds to the
antibody.
"Substitution" refers to the replacement of at least one nucleotide or amino
acid by a different
2 s nucleotide or amino acid.
"Substrate" refers to any suitable rigid or semi-rigid support including,
e.g., membranes, filters,
chips, slides, wafers, fibers, magnetic or nonmagnetic beads, gels, tubing,
plates, polymers,
microparticles or capillaries. The substrate can have a variety of surface
forms, such as wells,
trenches, pins, channels and pores, to which polynucleotides or polypeptides
are bound.
3 o A "transcript image" refers to the collective pattern of gene expression
by a particular tissue
or cell type under given conditions at a given time.
"Transformation" refers to a process by which exogenous DNA enters a recipient
cell.
Transformation may occur under natural or artificial conditions using various
methods well known in
24
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
the art. Transformation may rely on any known method for the insertion of
foreign nucleic acid
sequences into a prokaryotic or eukaryotic host cell. The method is selected
based on the host cell
being transformed.
"Transformants" include stably transformed cells in which the inserted DNA is
capable of
s replication either as an autonomously replicating plasmid or as part of the
host chromosome, as well as
cells which transiently express inserted DNA or RNA.
A "transgenic organism," as used herein, is any organism, including but not
limited to animals
and plants, in which one or more of the cells of the organism contains
heterologous nucleic acid
introduced by way of human intervention, such as by transgenic techniques well
known in the art. The
so nucleic acid is introduced into the cell, directly or indirectly by
introduction into a precursor of the cell,
by way of deliberate genetic manipulation, such as by microinjection or by
infection with a
recombinant virus. The term genetic manipulation does.not include classical
cross-breeding, or in vitro
fertilization, but rather is directed to the introduction of a recombinant DNA
molecule. The transgenic
organisms contemplated in accordance with the present invention include
bacteria, cyanobacteria,
is fungi, and plants and animals. The isolated DNA of the present invention
can be introduced into the
host by methods known in the art, for example infection, transfection,
transformation or
transconjugation. Techniques for transferring the DNA of the.present invention
into such organisms w
are widely known and provided in references such as Sambrook et al. (1989),
supra.
A "variant" of a parkicular nucleic acid sequence~is defined as a nucleic acid
sequence having
2 o at least 25% sequence identity to the particular nucleic acid sequence
over a certain length of one of
the nucleic acid sequences using blastn with the "BLAST 2 Sequences" tool
Version 2Ø9 (May-07-
1999) set at default parameters. Such a pair of nucleic acids may show, for
example, at least 30%, at
least 50%~ at least 60%, at least 70%, at least 80%, at least 90%, at least 91
%, at least 92%, at least
93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or
at least 99% or greater
25 sequence identity over a certain defined length. The variant may result in
"conservative" amino acid
changes which do not affect structural and/or chemical properties. A variant
may be described as, for
example, an "allelic" (as defined above), "splice," "species," or
"poiymorphic" variant. A splice
variant may have significant identity to a reference molecule, but will
generally have a greater or
lesser number of polynucleotides due to alternate splicing of exons during
mRNA processing. The
s o corresponding polypeptide may possess additional functional domains or
lack domains that are present
in the reference molecule. Species variants are polynucleotide sequences that
vary from one species
to another. The resulting polypeptides generally will have significant amino
acid identity relative to
each other. A polymorphic variant is a variation in the polynucleotide
sequence of a particular gene
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
between individuals of a given species. Polymorphic variants also may
encompass "single nucleotide
polymorphisms" (SNPs) in which the polynucleotide sequence varies by one base.
The presence of
SNPs may be indicative of, for example, a certain population, a disease state,
or a propensity for a
disease state.
In an alternative, variants of the polynucleotides of the present invention
may be generated
through recombinant methods. One possible method is a DNA shuffling technique
such as
MOLECULARBREEDING (Maxygen Inc., Santa Clara CA; described in U.S. Patent
Number
5,837,458; Chang, C.-C. et al. (1999) Nat. Biotechnol. 17:793-797; Christians,
F.C. et al. (1999) Nat.
Biotechnol. 17:259-264; and Crameri, A. et al. (1996) Nat: Biotechnol. 14:315-
319) to alter or improve
to the biological properties of SPTM, such as its biological or enzymatic
activity or its ability to bind to
other molecules or compounds. DNA shuffling is a process by which a library of
gene variants is
produced using PCR-mediated recombination of gene fragments. The library is
then subjected to
selection or screening procedures that identify those gene variants with the
desired properties. These
preferred variants may then be pooled and further subjected to recursive
rounds of DNA shuffling and
is selectionlscreening. Thus, genetic diversity is created through
"artificial" breeding and rapid molecular
evolution. For example, fragments of a single gene containing random point
mutations may be
recombined, screened, and then reshuffled until the desired properties are
optimized. Alternatively,
fragments of a given gene may be recombined with fragments of homologous genes
in the same gene
family, either from the same or different species thereby maximizing the
genetic diversity of multiple
2o naturally occurring genes in a.directed and controllable manner.
A "variant" of a particular polypeptide sequence is defined as a polypeptide
sequence having
at least 40-% sequence identity to the particular polypeptide sequence over a
certain length of one of
the, polypeptide sequences using blastp with the "BLAST 2 Sequences" tool
Version 2Ø9 (May-07-
1999) set at default parameters. Such a pair of polypeptides may show, for
example, at least 50%, at
25 least 60%, at least 70%, at least 80%, A "variant" of a particular
polypeptide sequence is defined as
a polypeptide sequence having at least 40% sequence identity to the particular
polypeptide sequence
over a certain length of one of the polypepdde sequences using blastp with the
"BLAST 2 Sequences"
tool Version 2Ø9 (May-07-1999) set at default parameters. Such a pair of
polypeptides may show,
for example, at least 50%, at least 60%, at least 70%, at least 80%, at least
90%, at Ieast 91 %, at least
so 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%,
at least 98%, or at least
99% or greater sequence identity over a certain defined length of one of the
polypeptides.
sequence identity over a certain defined length of.one of the polypeptides.
26
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THE INVENTION
In a particular embodiment, cDNA sequences derived from human tissues and cell
lines were
aligned based on nucleotide sequence identity and assembled into "consensus"
or "template"
sequences which are designated by the template identification numbers
(template IDs) in column 2 of
s Table 2. The sequence identification numbers (SEQ ID NOa) corresponding to
the template IDs are
shown in column 1. Segments of the template sequences are defined by the
"start' and "stop"
nucleotide positions listed in columns 3 and 4. These segments, when
translated in the reading frames
indicated in column 5, have similarity to signal peptide (SP) or transmembrane
(TM) domain consensus
sequences, as indicated in column 6.
to The invention incorporates the nucleic acid sequences of these templates as
disclosed in the
Sequence Listing and the use of these sequences in the diagnosis and treatment
of disease states
characterized by defects in cell signaling. The invention further utilizes
these sequences in
hybridization and amplification technologies, and in particular, in
technologies which assess gene
expression patterns correlated with specific cells or tissues and their
responses in vivo or in vitro to
is pharmaceutical agents, toxins, and other treatments. In this manner, the
sequences of the present
invention are used to develop a transcript image for a particular cell or
tissue.
Derivation of Nucleic Acid Sequences
cDNA was isolated from libraries constructed using RNA derived from normal and
diseased
a o human tissues and cell lines. The human tissues and cell lines used for
cDNA library construction
were selected from a broad range of sources to provide a diverse population of
cDNAs representative
of gene transcription throughout the human body. Descriptions of the human
tissues and cell lines
used for cDNA library construction are provided in the LIFESEQ database
(Incyte Genomics, Inc.
(Incyte), Palo Alto CA). Human tissues were broadly selected from, for
example, cardiovascular;
25 dermatologic, endocrine, gastrointestinal, hematopoietic/immune system,
musculoskeletal, neural,
reproductive, and urologic sources.
Cell lines used for cDNA library construction were derived from, for example,
leukemic cells,
teratocarcinomas, neuroepitheliomas, cervical carcinoma, lung fibroblasts, and
endothelial cells. Such
cell lines include, for example, THP-1, Jurkat, HUVEC, hNT2,, WI38, HeLa, and
other cell lines
3 o commonly used and available from public depositories (American Type
Culture Collection, Manassas
VA). Prior to mRNA isolation, cell lines were untreated, treated with a
pharmaceutical went such as
5'-aza-2'-deoxycytidine, treated with an activating agent such as
lipopolysaccharide in the case of
leukocytic cell lines, or, in the case of endothelial cell lines, subjected to
shear stress.
27
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WO 02/20756 PCT/USO1/27297
Sequencing of the cDNAs
Methods for DNA sequencing are well known in the art. Conventional enzymatic
methods
employ the Klenow fragment of DNA polymerase I, SEQUENASE DNA polymerase (U.S.
s Biochemical Corporation, Cleveland OH), Taq polymerase (Applied Biosystems,
Foster City CA),
thermostable T7 polymerase (Amersham Pharmacia Biotech, Inc. (Amersham
Pharmacia Biotech),
Piscataway NJ), or combinations of polymerases and proofreading exonucleases
such as those found
in the ELONGASE amplification system (Life Technologies Inc. (Life
Technologies), Gaithersburg
MD), to extend the nucleic acid sequence from an oligonucleotide primer
annealed to the DNA
to template of interest. Methods have been developed for the use of both
single-stranded and double-
stranded templates. Chain termination reaction products may be electrophoresed
on urea-
polyacrylamide gels and detected either by autoradiography (for radioisotope-
labeled nucleotides) or
by fluorescence (for fluorophore-labeled nucleotides). Automated methods for
mechanized reaction
preparation; sequencing, and analysis using fluorescence detection methods
have been developed.
is Machines used to prepare cDNAs for sequencing can include the MICROLAB 2200
liquid transfer
system (Hamilton Company (Hamilton), Reno N~, Pettier thermal cycler (PTC200;
MJ Research,
Inc. (MJ Research), Watertown MA), and ABI CATALYST 800 thermal eycler
(Applied
Biosystems). Sequencing can be carried out using, for example, the ABI 373 or
377 (Applied
Biosystems) or MEGABACE 1000 (Molecular Dynamics, Inc. (Molecular Dynamics),
Sunnyvale
2 o CA) DNA sequencing systems, or other automated and manual sequencing
systems well known in the
art.
The nucleotide sequences of the Sequence Listing have been prepared by
current, state-of
the-art, automated methods and, as such, may contain occasional sequencing
errors or unidentified
nucleotides. Such unidentified nucleotides are designated by an N. These
infrequent unidentified
a s bases do not represent a hindrance to practicing the invention for those
skilled in the art. Several
methods employing standard recombinant techniques maybe used to correct errors
and complete the
missing sequence information. (See, e.g., those described in Ausubel, F.M. et
al. (1997) Short
Protocols in Molecular Biolo~y, John Wiley & Sons, New York NY; and Sambrook,
J. et al. (1989)
Molecular Cloning, A Laboratory Manual, Cold Spring 'Harbor Press, Plainview
NY.)
Assembly of cDNA Seguences
Human polynucleotide sequences may be assembled using programs or algorithms
well known
in the art. Sequences to be assembled are related, wholly or in part, and may
be derived from a single
28
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
or many different transcripts. Assembly of the sequences can be performed
using such programs as
PHRAP (Phils Revised Assembly Program) and the GELVIEW fragment assembly
system (GCG),
or other methods known in the art.
Alternatively, cDNA sequences are used as "component" sequences that are
assembled into
s "template" or "consensus" sequences as follows. Sequence chromatograms are
processed, verified,
and quality scores are obtained using PHRED. Raw sequences are edited using an
editing pathway
known as Block 1 (See, e.g., the LIFESEQ Assembled User Guide, Incyte
Genomics, Palo Alto, CA).
A series of BLAST comparisons is performed and low-information segments and
repetitive elements
(e.g., dinucleotide repeats, Alu repeats, etc.) are replaced by "n's", or
masked, to prevent spurious
to matches. Mitochondria) and ribosomal RNA sequences are also removed. The
processed sequences
are then loaded into a relational database management system (RDMS) which
assigns edited
sequences to existing templates, if available. When additional sequences are
added into the RDMS, a
process is initiated which modifies existing templates or creates new
templates from works in progress
(i.e., nonfmal assembled sequences) containing queued sequences or the
sequences themselves.
is After the new sequences have been assigned to templates, the templates can
be merged into bins. If
,multiple templates exist in,one bin, the bin can be split and the templates
reannotated.
Once gene bins have been generated based upon sequence alignments, bins are
"clone joined"
based upon clone information. Clone joining occurs when the 5' sequence of one
clone is present in
one bin and the 3' sequence from the same clone is present in a different bin,
indicating that the two
2 o bins should be merged into a single bin. Only bins which share at least
two different clones are
merged.
A resultant template sequence may contain either a partial or a full length
open reading frame,
or all or part of a genetic regulatory element. This variation is due in part
to the fact that the full
length cDNAs of many genes are several hundred, and sometimes several
thousand, bases in length.
25 With current technology, cDNAs comprising the coding regions of large genes
cannot be cloned
because of vector limitations, incomplete reverse transcription of the mRNA,
or incomplete "second
strand" synthesis. Template sequences may be extended to include additional
contiguous sequences
derived from the parent RNA transcript using a variety of methods known to
those of skill in the art.
Extension may thus be used to achieve the full length coding sequence of a
gene.
Analysis of the cDNA Sequences
The cDNA sequences are analyzed using a variety of programs and algorithms
which are
well known in the art. (See, e.g., Ausubel, 1997, supra, Chapter 7.7; Meyers,
R.A. (Ed.) (1995)
29
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
Molecular Biology and Biotechnology, Wiley VCH, New York NY> pp. 856-853; and
Table 6.) These
analyses comprise both reading frame determinations, e.g., based on triplet
codon periodicity for
particular organisms (Fickett, J.W. (1982) Nucleic Acids Res. 10:5303-5318);
analyses of potential
start and stop codons; and homology searches.
Computer programs known to those of skill in the art for performing computer-
assisted
searches for amino acid and nucleic acid sequence similarity, include, for
example, Basic Local
Alignment Search Tool (BLAST; Altschul, S.F. (1993) J. Mol. Evol. 36:290-300;
Altschul, S.F. et al.
(1990) J. Mol. Biol. 215:403-410). BLAST is especially useful in determining
exact matches and
comparing two sequence fragments of arbitrary but equal lengths, whose
alignment is locally maximal
to and for which the alignment score meets or exceeds a threshold or cutoff
score set by the user
(Karlin, S. et al. (1988) Proc. Nat!. Acad. Sci. USA 85:841-845). Using an
appropriate search tool
(e.g., BLAST or HMM), GenBank, SwissProt, BLOCKS, PFAM and other databases may
be
searched for sequences containing regions of homology to a query sptm or SPTM
of the present
invention.
Other approaches to the identification, assembly, storage, and display of
nucleotide and
polypeptide sequences are provided in "Relational Database for Storing
Biomolecule Information,"
U.S.S.N. 08/947,845, filed October 9, 1997; "Project-Based Full-Length
Biomolecular Sequence
Database," U.S.S.N. 08/811,758, filed March 6, 1997; and "Relational Database
and System for
Storing Information Relating to Biomolecular Sequences," U.S.S.N. 09/034,807,
filed:March 4; 1998,
2 o all of which are incorporated by reference herein in their entirety.
Protein hierarchies can be assigned to the putative encoded polypeptide based
ori, e.g., motif,
BLAST, or biological analysis. Methods for assigning these hierarchies are
described, for example, in
"Database System Employing Protein Function Hierarchies for Viewing
Biomolecular Sequence
Data," U.S.S.N. 08/812,290, filed March 6, 1997, incorporated herein by
reference.
Human Secretor's Sequences
The sptm of the present invention may be used for a variety of diagnostic ,and
therapeutic
purposes. For example, an sptm may be used to diagnose a particular condition,
disease, or disorder
associated with cell signaling. Such conditions, diseases, and disorders
include, but are not limited to, a
3 o cell proliferative disorder such as actinic keratosis, arteriosclerosis,
atherosclerosis, bursitis, cirrhosis,
hepatitis, mired connective tissue disease (MCTD), myelofibrosis, paroxysmal
nocturnal
hemoglobinuria, polycythemia vera, psoriasis, primary thrombocythemia, and
cancers including
adenocarcinoma, leukemia, lymphoma, melanoma, myeloma, sarcoma,
teratocarcinoma, and, in
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
particular, a cancer of the adrenal gland, bladder, bone, bone marrow, brain,
breast, cervix, gall
bladder, ganglia, gastrointestinal tract, heart, kidney, liver, lung, muscle,
ovary, pancreas, parathyroid,
penis, prostate, salivary glands, skin, spleen, testis, thymus, thyroid, and
uterus; an immune system
disorder such as such as inflammation, actinic keratosis, acquired
immunodeficiency syndrome
s (AIDS), Addison's disease, adult respiratory distress syndrome, allergies,
ankylosing spondylitis,
amyloidosis, anemia, arteriosclerosis, asthma, atherosclerosis, autoimmune
hemolytic anemia,
autoimmune thyroiditis, bronchitis, bursitis, cholecystitis, cirrhosis,
contact dermatitis, Crohn's disease,
atopic dermatitis, dermatomyositis, diabetes mellitus, emphysema,
erythroblastosis fetalis, erythema
nodosum, atrophic gastritis, glomerulonephritis, Goodpasture's syndrome, gout,
Graves' disease,
to Hashimoto's thyroiditis, paroxysmal nocturnal hemoglobinuria, hepatitis,
hypereosinophilia, irritable
bowel syndrome, episodic lymphopenia with lymphocytotoxins, mixed connective
tissue disease
(MCTD), multiple sclerosis, myasthenia gravis, myocardial or pericardial
inflammation, myelofibrosis,
osteoarthritis, osteoporosis, pancreatitis, polycythemia vera, polymyositis,
psoriasis, Reiter's syndrome,
rheumatoid arthritis, scleroderma, Sjogren's syndrome, systemic anaphylaxis,
systemic lupus
15 erythematosus, systemic sclerosis, primary thrombocythemia,
thrombocytopenic purpura, ulcerative
colitis, uveitis, Werner syndrome, complications of cancer, hemodialysis, and
extracorporeal
circulation, trauma, and hematopoietic cancer including lymphoma, leukemia,
and myeloma; and a
neurological disorder such as epilepsy, ischemic cerebrovascular disease,
stroke, cerebral neoplasms,
Alzheimer's disease, Pick's disease, Huntington's disease, dementia,
Parkinson's disease and other
2 o extrapyramidal disorders, amyotrophic lateral sclerosis and other motor
neuron disorders, progressive
neural muscular atrophy, retinitis pigmentosa, hereditary ataxias, multiple
sclerosis and other
demyelinating diseases; bacterial and viral meningitis, brain abscess,
subdural empyema, epidural
abscess, suppurative intracranial thrombophlebitis, myelitis and radiculitis,
viral central nervous system
disease, prion diseases including kuru, Creutzfeldt-Jakob disease, and
Gerstmann-Straussler-Scheinker
2 s syndrome, fatal familial insomnia, nutritional and metabolic diseases of
the nervous system,
neurofibromatosis, tuberous sclerosis, cerebelloretinal hemangioblastomatosis,
encephalotrigeminal
syndrome, mental retardation and other developmental disorder of the central
nervous system, cerebral
palsy, a neuroskeletal disorder, an autonomic nervous system disorder, a
cranial nerve disorder, a
spinal cord disease, muscular dystrophy and other neuromuscular disorder, a
peripheral nervous
s o system disorder, dermatomyositis and polymyositis, inherited, metabolic,
endocrine, and toxic
myopathy, myasthenia gravis, periodic paralysis, a mental disorder including
mood, anxiety, and
schizophrenic disorder, seasonal affective disorder (SAD), akathesia, amnesia,
catatonia,' diabetic
neuropathy, tardive dyskinesia, dystonias, paranoid psychoses, postherpetic
neuralgia, and Tourette's
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disorder. The sptm can be used to detect the presence of, or to quantify the
amount of, an sptm-
related polynucleotide in a sample. This information is then compared to
information obtained from
appropriate reference samples, and a diagnosis is established. Alternatively,
a polynucleotide
complementary to a given sptm can inhibit or inactivate a therapeutically
relevant gene related to the
s sptm.
Analysis of sptm Expression Patterns
The expression of sptm may be routinely assessed by hybridization-based
methods to
determine, for example, the tissue-specificity, disease-specificity, or
developmental stage-specificity of
to sptm expression. For example, the level of expression of sptm may be
compared among different cell
types or tissues, among diseased and normal cell types or tissues, among cell
types or tissues at
different developmental stages, or among cell types or tissues undergoing
various treatments. This
type of analysis is useful, for example, to assess the relative levels of sptm
expression in fully or
partially differentiated cells or tissues; to determine if changes in sptm
expression levels are correlated
is with the development or progression of specific disease states, and to
assess the response of a cell or
tissue to a specific therapy, for example, in pharmacological or toxicological
studies. Methods for the
analysis of sptm expression are based on hybridization and amplification
technologies and include .
membrane-based procedures such as northern blot analysis, high-throughput
procedures that utilize, for
example, microarrays, and PCR-based procedures.
Hybridization and Genetic Analysis
The sptm, their fragments; or complementary sequences, may be used to identify
the presence
of andlor to determine the degree of similarity between two (or more) nucleic
acid sequences. The
sptm may be hybridized to naturally occurring or recombinant nucleic acid
sequences under
appropriately selected temperatures and salt concentrations. Hybridization
with a probe based on the
nucleic acid sequence of at least one of the sptm allows for the detection of
nucleic acid sequences,
including genomic sequences, which are identical or related to the sptm of the
Sequence Listing. ,
Probes may be selected from non-conserved or unique regions of at least one of
the polynucleotides of
SEQ ID N0:~1-184 and tested for, their ability to identify or amplify the
target nucleic acid sequence
3 o using standard protocols.
Polynucleotide sequences that are capable of hybridizing, in particular, to
those shown in SEQ
ID NO:1-184 and fragments thereof, can be identified using various conditions
of stringency. (See,
e.g., Wahl, G.M. and S.L. Berger (1987) Methods Enzymol. 152:399-407; Kimmel,
A:R. (1987)
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Methods Enzymol. 152:507-51 l.) Hybridization conditions are discussed in
"Definitions."
A probe for use in Southern or northern hybridization may be derived from a
fragment of an
sptm sequence, or its complement, that is up to several hundred nucleotides in
length and is either
single-stranded or double-stranded. Such probes may be hybridized in solution
to biological materials
s such as plasmids, bacterial, yeast, or human artificial chromosomes, cleared
or sectioned tissues, or to
artificial substrates containing sptm. Microarrays are particularly suitable
for identifying the presence
of and detecting the level of expression for multiple genes of interest by
examining gene expression
correlated with, e.g., various stages of development, treatment with a drug or
compound, or disease
progression. An array analogous to a dot or slot blot may be used to arrange
and link polynucleotides
to to the surface of a substrate using one or more of the following:
mechanical (vacuum), chemical,
thermal, or UV bonding procedures. Such an array may contain any number of
sptm and may be
produced by hand or by using available devices, materials, and machines.
Microarrays may be prepared, used, and analyzed using methods known in the
art. (See, e.g.,
Brennan, T.M. et al. (1995) U.S. Patent No. 5,474,796; Schena, M. et al.
(1996) Proc. Natl. Acad.
15 Sci. USA 93:10614-10619; Baldeschweiler et al. (1995) PCT application
WO95/251116; Shalom D. et
al. (1995) PCT application W095/35505; Heller, R.A. et al. (1997) Proc. Natl.
Acad. Sci. USA
94:2150-2155; and Heller, M.J. et al. (1997) U.S. Patent No. 5,605,662.)
Probes may be labeled by either PCR or enzymatic techniques using a variety of
commercially available reporter molecules. For example, commercial kits are
available for radioactive
2 o and chemiluminescent labeling (Amersham Pharmacia Biotech) and for
alkaline phosphatase labeling
(Life Technologies). Alternatively, sptm may be cloned into commercially
available vectors for the
production of RNA probes. Such probes may be transcribed in the presence of at
least one labeled
nucleotide (e.g., 32P-ATP, Amersham Pharmacia Biotech).
Additionally the polynucleotides of SEQ ID N0:1-1 S4 or suitable fragments
thereof can be
a s used to isolate full length cDNA sequences utilizing hybridization and/or
amplification procedures well
known in the art, e.g., cDNA library screening, PCR amplification, etc. The
molecular cloning of such
full length cDNA sequences may employ the method of cDNA library screening
with probes using the ,
hybridization, stringency, washing, and probing strategies described above and
in Ausubel, supra,
Chapters 3, 5,' and 6. These procedures may also be employed with genomic
libraries to isolate
s o genomic sequences of sptm in order to analyze, e.g., regulatory elements.
Genetic Mapping
Gene identification and mapping are important in the investigation and
treatment of almost all
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conditions, diseases, and disorders. Cancer, cardiovascular disease,
Alzheimer's disease, arthritis,
diabetes, and mental illnesses are of particular interest. Each of these
conditions is more complex than
the single gene defects of sickle cell anemia or cystic fibrosis, with select
groups of genes being
predictive of predisposition for a particular condition, disease, or disorder.
For example,
s cardiovascular disease may result from malfunctioning receptor molecules
that fail to clear cholesterol
from the bloodstream, and diabetes may result when a particular individual's
immune system is
activated by an infection and attacks the insulin-producing cells of the
pancreas. In.some studies,
Alzheimer's disease has been linked to a gene on chromosome 21; other studies
predict a different
gene and location. Mapping of disease genes is a complex and reiterative
process and generally
to proceeds from genetic linkage analysis to physical mapping.
As a condition is noted among members of a family, a genetic linkage map
traces parts of
chromosomes that are inherited in the same pattern as the condition.
Statistics link the inheritance of
particular conditions to particular regions of chromosomes, as defined by RFLP
or other markers.
(See, for example, Larder, E. S. and Botstein, D. (1986) Proc. Natl. Acad.
Sci. USA 83:7353-7357.)
15 Occasionally, genetic markers and their locations are known from previous
studies. More often,
however, the markers are simply stretches of DNA that differ among
individuals. Examples of
genetic linkage maps can be found in various scientific journals or at the
Online Mendelian Inheritance
in Man (OMIM) World Wide Web site.
In another embodiment of the invention, sptm sequences may be used to generate
2 o hybridization probes useful in chromosomal mapping of naturally occurring
genomic sequences. Either
coding or noncoding sequences of sptm may be used, and in some instances,
noncoding sequences
may be preferable over coding sequences. For example, conservation of an sptm
coding sequence
among members of a multi-gene family may potentially cause undesired cross
hybridization during
chromosomal mapping. The sequences may be mapped to a particular chromosome,
to a specific
25 region of a chromosome, or to artificial chromosome constructions, e.g.,
human artificial chromosomes
(HACs), yeast artificial chromosomes (YACs), bacterial artificial chromosomes
(BACs), bacterial P1
constructions, or single chromosome cDNA libraries. (See, e.g:, Harrington,
J.J. et al. (1997) Nat.
Genet. 15:345-355; Price, C.M. (1993) Blood Rev. 7:127-134; and Trask, B.J.
(1991) Trends Genet.
7:149-154.)
s o Fluorescent in situ hybridization (FISH) may be correlated with other
physical chromosome
mapping techniques and genetic map data. (See, e.g., Meyers, supra, pp. 965-
968.) Correlation
between the location of sptm on a physical chromosomal map and a specific
disorder, or a
predisposition to a specific disorder, may help define the region of DNA
associated with that disorder.
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The sptm sequences may also be used to detect polymorphisms that are
genetically linked to the
inheritance of a particular condition, disease, or disorder.
In situ hybridization of chromosomal preparations and genetic mapping
techniques, such as
linkage analysis using established chromosomal markers, may be used for
extending existing genetic
s maps. Often the placement of a gene on the chromosome of another mammalian
species, such as
mouse, may reveal associated markers even if the number or arm of the
corresponding human
chromosome is not known. These new marker sequences can be mapped to human
chromosomes
and may provide valuable information to investigators searching for disease
genes using positional
cloning or other gene discovery techniques. Once a disease or syndrome has
been crudely correlated
to by genetic linkage with a particular genomic region, e.g., ataxia-
telangiectasia to 11q22-23, any
sequences mapping to that area may represent associated or regulatory genes
for further investigation.
(See, e.g., Gatti, R.A. et al. (1988) Nature 336:577-580.) The nucleotide
sequences of the subject
invention may also be used to detect differences in chromosomal architecture
due to translocation,
inversion, etc., among normal, carrier, or affected individuals.
15 Once a disease-associated gene is mapped to a chromosomal region, the gene
must be cloned
in order to identify mutations or other alterations (e.g., translocations or
inversions) that may be
correlated with disease. This process requires a physical map of the
chromosomal region containing
the disease-gene of interest along with associated markers. A physical map is
necessary for
determining the nucleotide sequence of and order of marker genes on a
particular chromosomal
2 o region. Physical mapping techniques are well known in the art and require
the generatibn of
overlapping sets of cloned DNA fragments from a particular organelle,
chromosome, or genome.
These clones are analyzed to reconstruct and catalog their order. Once the
position of a marker is
determined, the DNA from that region is obtained byconsulting the catalog and
selecting clones from
that region. The gene of interest is located through positional cloning
techniques using hybridization or
2s similar methods.
Diagnostic Uses
The sptm of the present invention may be used to design probes useful in
diagnostic assays.
Such assays, well known to those skilled in the art, may be used to detect or
confirm conditions,
3 o disorders, or diseases associated with abnormal levels of sptm expression.
Labeled probes developed
from sptm sequences are added to a sample under hybridizing conditions of
desired stringency. In
some instances, sptm, or fragments or oligonucleotides derived from sptm, may
be used as primers in
amplification steps.prior to hybridization. The amount of hybridization
complex formed is quantified
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
and compared with standards for that cell or tissue. If sptm expression varies
significantly from the
standard, the assay indicates the presence of the condition, disorder, or
disease. Qualitative or
quantitative diagnostic methods may include northern, dot blot, or other
membrane or dip-stick based
technologies or multiple-sample format technologies such as PCR, enzyme-linked
immunosorbent
s assay (ELISA)-like, pin, or chip-based assays.
The probes described above may also be used to monitor the progress of
conditions, disorders,
or diseases associated with abnormal levels of sptm expression, or to evaluate
the efficacy of a
particular therapeutic treatment. The candidate probe may be identified from
the sptm that are
specific to a given human tissue and have not been observed in GenBank or
other genome databases.
to Such a probe rnay be used in animal studies, preclinical tests, clinical
trials, or in monitoring the
treatment of an individual patient. In a typical process, standard expression
is established by methods
well known in the art for use as a basis of comparison, samples from patients
affected by the disorder
or disease are combined with the probe to evaluate any deviation from the
standard profile, and a
therapeutic agent is administered and effects are monitored to generate a
treatment profile. Efficacy
is is evaluated by determining whether the expression progresses toward or
returns to the standard
normal pattern. Treatment profiles may be generated over a period of several
days or several months.
Statistical methods well known to those skilled in the art may be use to
determine the significance of
such therapeutic agents.
The polynucleotides are also useful for identifying individuals from minute
biological samples;
2 o for example, by matching the RFLP pattern of a sample's~DNA to that of an
individual's DNA. The
polynucleotides of the present invention can also be used to determine the
actual base-by-base DNA ..
sequence of selected portions-of an individual's genome. These sequences can
be used to prepare
PCR primers for amplifying and isolating such selected DNA, which can then be
sequenced. Using
this technique, an individual can be identified through a unique set of DNA
sequences. Once a unique
25 )D database is established for an individual, positive identification of
that individual can be made from
extremely small tissue samples.
In a particular aspect, oligonucleotide primers derived from the sptm of the
invention may be
used to detect single nucleotide polymorphisms (SNPs). SNPs are substitutions,
insertions and
deletions .:hat are a frequent cause of inherited or acquired genetic disease
in humans. Methods of
a o SNP detection include, but are not limited to, single.-stranded
conformation polymorphism (SSCP) and
fluorescent SSCP (fSSCP) methods. In SSCP, oligonucleotide primers derived
from sptm are used to
amplify DNA using the polymerase chain reaction (PCR). The DNA may be derived,
for example,
from diseased or normal tissue, biopsy samples, bodily fluids, and the like.
SNPs in the DNA cause
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WO 02/20756 PCT/USO1/27297
differences in the secondary and tertiary structures of PCR products in single-
stranded form, and
these differences are detectable using gel electrophoresis in non-denaturing
gels. In fSCCP, the
oligonucleotide primers are fluorescently labeled, which allows detection of
the amplimers in high-
throughput equipment such as DNA sequencing machines. Additionally, sequence
database analysis
s methods, termed in silico SNP (isSNP), are capable of identifying
polymorphisms by comparing the
sequences of individual overlapping DNA fragments which assemble into a common
consensus
sequence. These,computer-based methods filter out sequence variations due to
laboratory preparation
of DNA and sequencing errors using statistical models and automated analyses
of DNA sequence
chromatograms. In the alternative, SNPs may be detected and characterized by
mass spectrometry
to using, for example, the high throughput MASSARRAY system (Sequenom, Inc.,
San Diego CA).
DNA-based identification techniques are critical in forensic technology. DNA
sequences
taken from very small biological samples such as tissues, e.g., hair or skin,
or body fluids, e.g., blood,
saliva, semen, etc., can be amplified using, e.g., PCR, to identify
individuals. (See, e.g., Erlich, 'H.
(1992) PCR Technolo~y, Freeman and Co., New York, NY). Similarly,
polynucleotides of the present
is invention can be used as polymorphic markers.
There is also a need for reagents capable of identifying the source of a
particular tissue.
Appropriate reagents can comprise, for example, DNA probes or primers prepared
from the
sequences of the present invention that are specific for particular tissues.
Panels of such reagents can
identify tissue by species and/or by organ type. In a similar fashion, these
reagents can be used to
2 o screen tissue cultures for contamination.
The polynucleotides of the present invention can also be used as molecular
weight markers on
nucleic acid gels or Southern blots, as diagnostic probes for the presence of
a specific mRNA in a
particular cell type, in the creation of subtracted cDNA libraries which aid
in the discovery of novel
polynucleotides, in selection and synthesis of oligomers for attachment to an
array or other support,
25 and as an antigen to elicit an immune response.
Disease Model Systems Using sntm
The polynucleotides encoding SPTM or their mammalian homologs may be "knocked
out" in
an animal model system using homologous recombination in embryonic stem (ES)
cells. Such
s o techniques are well known in the art and are useful for the generation of
animal models of human
disease. (See, e.g., U.S. Patent Number 5,175,353 and U.S. Patent Number
5,767,337.) For example,
mouse ES cells, such as the mouse 129/SvJ cell lined are derived from the
early mouse embryo and
grown in culture. The ES cells are transformed with a vector containing the
gene of interest disrupted
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CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
by a marker gene, e.g., the neomycin phosphotransferase gene (neo; Capecchi,
M.R.~(1989) Science
244:1288-1292). The vector integrates into the corresponding region of the
host genome by
homologous recombination. Alternatively, homologous recombination takes place
using the Cre-loxP
system to knockout a gene of interest in a tissue- or developmental stage-
specific manner (March, J.D.
s (1996) Clin. Invest. 97:1999-2002; Wagner, K.U. et al. (1997) Nucleic Acids
Res. 25:4323-4330).
Transformed ES cells are identified and microinjected into mouse cell
blastocysts such as those from
the C57BL/6 mouse strain. The blastocysts are surgically transferred
to~pseudopregnant dams, and
the resulting chimeric progeny are genotyped and bred to produce heterozygous
or homozygous
strains. Transgenic animals thus generated may be tested with potential
therapeutic or toxic agents.
so The polynucleotides encoding SPTM may also be manipulated in vitro in ES
cells derived from
human blastocysts. Human ES cells have the potential to differentiate into at
least eight separate cell
lineages including endoderm, mesoderm, and ectodermal cell types. These cell
lineages differentiate
into, for example, neural cells, hematopoietic lineages, and cardiomyocytes
(Thomson, J.A. et al.
(1998) Science 282:1145-1147).
15 The polynucleotides encoding SPTM of the invention can also be used to
create "knockin"
humanized animals (pigs) or transgenic animals (mice or rats) to model human
disease. With knockin ..
technology, a region of sptm is injected into animal ES cells, and the
injected sequence integrates into
the animal cell genome. Transformed cells are injected into blastulae, and the
blastulae are implanted
as described above. Transgenic progeny or inbred lines are studied and treated
with potential
2 o pharmaceutical agents to obtain information on treatment of a human
disease. Alternatively, a
mammal inbred to overexpress sptm, resulting, e.g., in the secretion of SPTM
in its milk, may also
serve as a convenient source of that protein (Janne, J. et al. (1998)
Biotechnol. Annu. Rev. 4:55-74).
Screening Assays
25 SPTM encoded by polynucleotides of the present invention may be used to
screen for
molecules that bind to or are bound by the encoded polypeptides. The binding
of the polypeptide and
the molecule may activate (agonist), increase, inhibit (antagonist), or
decrease activity of the
polypeptide or the bound molecule. Examples of such molecules include
antibodies, oligonucleotides,
proteins (e.g., receptors) or small molecules.
3 o Preferably, the molecule is closely related to the natural ligand of the
polypeptide, e.g., a ligand
or fragment thereof, a natural substrate, or a structural or functional
mimetic. (See, Coligan et al.,
(1991) Current Protocols in Immunolo~y 1 (2): Chapter 5.) Similarly, the
molecule can be closely
related to the natural receptor to which the polypeptide binds, or to at least
a fragment of the receptor,
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CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
e.g., the active site. In, either case, the molecule can be rationally
designed using known techniques.
Preferably, the screening for these molecules involves producing appropriate
cells which express the
polypeptide, either as a secreted protein or on the cell membrane. Preferred
cells include cells from
mammals, yeast, Drosophila, or E. coli. Cells expressing the polypeptide or
cell membrane fractions
s which contain the expressed polypeptide are then contacted with a test
compound and binding,
stimulation, or inhibition of activity of either the polypeptide or the
molecule is analyzed.
An assay may simply test binding of a candidate compound to the polypeptide,
wherein binding
is detected by a fluorophore, radioisotope, enzyme conjugate, or other
detectable label. Alternatively,
the assay may assess binding in the presence of a labeled competitor.
to Additionally, the assay can be carried out using cell-free preparations,
polypeptide/molecule
affixed to a solid support, chemical libraries, or natural product mixtures.
The assay may also simply
comprise the steps of mixing a candidate compound with a solution containing a
polypeptide,
measuring polypeptide/molecule activity or binding, and comparing the
polypeptide/molecule activity or
binding to a standard.
15 Preferably, an ELISA assay using, e.g., a monoclonal or polyclonal
antibody, can measure
polypeptide level in a sample. The antibody can measure polypeptide level by
either binding, directly
or indirectly, to the.polypeptide or by competing with the polypeptide for a
substrate. -
All of the above assays can be used in a diagnostic or prognostic context. The
molecules
discovered using these assays can be used to treat disease or to bring about a
particular result in a
2o patient (e.g., blood vessel growth) by activating or inhibiting the
polypeptide/molecule. Moreover, the
assays can discover agents which may inhibit or enhance the production of the
polypeptide from
suitably manipulated cells or tissues.
Transcript Ima~in~ and Toxicological Testing
2 s Another embodiment relates to the use of sptm to develop a transcript
image of a tissue or cell
type. A transcript image represents the global pattern of gene expression by a
particular tissue or cell
type. Global gene expression patterns are analyzed by quantifying the number
of expressed genes and
their relative abundance under given conditions and at a given time. (See
Seilhamer et al.,
"Comparative Gene Transcript Analysis," U.S. Patent Number 5,840,484,
expressly incorporated by
s o reference herein.) Thus a transcript image may be generated by hybridizing
the polynucleotides of the
present invention or their complements to the totality of transcripts or
reverse transcripts of a
particular tissue or cell type. In one embodiment, the hybridization takes
place in high-throughput
_ format, wherein the polynucleotides of the present invention or their
complements comprise a subset
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CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
of a plurality of elements on a microarray. The resultant transcript image
would provide a profile of
gene acti~rity pertaining to cell signaling.
Transcript images which profile sptm expression may be generated using
transcripts isolated
from tissues, cell lines, biopsies, or other biological samples. The
transcript image may thus reflect
s sptm expression iri vivo, as in the case of a tissue or biopsy sample, or in
vitro, as in the case of a cell
line.
Transcript images which profile sptm expression may also be used in
conjunction with in vitro
model systems and preclinical evaluation of pharmaceuticals, as well as
toxicological testing of
industrial and naturally-occurring environmental compounds. All compounds
induce characteristic
to gene expression patterns, frequently termed molecular fingerprints or
toxicant signatures, which are
indicative of mechanisms of action and toxicity (Nuwaysir, E. F. et al. (1999)
Mol. Carcinog. 24:153-
159; Steiner, S. and Anderson, N. L. (2000) Toxicol. Lett. 112-113:467-71,
expressly incorporated by
reference herein). If a test compound has a signature similar to that of a
compound with known
toxicity, it is likely to share those toxic properties. These fingerprints or
signatures are most useful and
15 refined when they contain expression information from a large number of
genes and gene families.
Ideally, a genome-wide measurement of expression provides the highest quality
signature. Even
genes whose expression is not altered by any tested compounds are important as
well, as the levels of
expression of these genes are used to normalize the rest of the expression
data. The normalization
procedure is useful for comparison of expression data after treatment with
different compounds.
a o While the assignment of gene function to elements of a toxicant signature
aids in interpretation of
toxicity mechanisms, knowledge of gene function is not necessary for the
statistical matching of
signatures which leads to prediction of toxicity. (See, for example, Press
Release 00-02 from the
National Institute of Environmental Health Sciences, released February 29,
2000, available at
http://www.niehs.nih.gov/oc/news/toxchip.htm.) Therefore, it is important and
desirable in
2s toxicological screening using toxicant signatures to include all expressed
gene sequences.
In one embodiment, the toxicity of a test compound is assessed by treating a
biological sample
containing nucleic acids with the test compound. Nucleic acids that are
expressed in the treated
biological sample are hybridized with one or more probes specific to the
polynucleotides of the present
invention, so that transcript levels corresponding to the polynucleotides of
the present invention may be
3 o quantified. The transcript levels in the treated biological sample are
compared with levels in an
untreated biological sample. Differences in the transcript levels between the
two samples are
indicative of a toxic response caused by the test compound in the treated
sample.
Another particular embodiment relates to the use of SPTM encoded by
polynucleotides of the
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
present invention to analyze the proteome of a tissue or cell type. The term
proteome refers to the
global pattern of protein expression in a particular tissue or cell type. Each
protein component of a
proteome can be subjected individually to further analysis. Proteome
expression patterns or profiles,
are analyzed by quantifying the number of expressed proteins and their
relative abundance under
s given conditions and at a given time. A profile of a cell's proteome may
thus be generated by
separating and analyzing the polypeptides of a particular tissue or cell type.
In one embodiment, the
separation is achieved using two-dimensional gel electrophoresis, in which
proteins from a sample are
separated by isoelectric focusing in the first dimension, and then according
to molecular weight by
sodium dodecyl sulfate slab gel electrophoresis in the second dimension
(Steiner and Anderson,
to , supra). The proteins are visualized in the gel as discrete and uniquely
positioned spots, typically by
staining the gel with an agent such as Coomassie Blue or silver or fluorescent
stains. The optical
density of each protein spot is generally proportional to the level of the
protein in the sample. The
optical densities of equivalently positioned protein spots from different
samples, for example, from
biological samples either treated or untreated with a test compound or
therapeutic agent, are
is compared to identify' any changes in protein spot density related to the
treatment. The proteins in the
spots are partially sequenced using, for example, standard methods employing
chemical or enzymatic:
cleavage followed by mass spectrometry. The identity of the protein in a spot
may be determined by .
comparing its partial sequence, preferably of at least 5 contiguous amino acid
residues, to the
polypeptide sequences of the present invention. In some cases, further
sequence data may be
20 obtained for definitive protein identification.
A proteoriiic profile may also be generated using antibodies specific for SPTM
to quantify the
levels of SPTM expression. In one embodiment, the antibodies are used as
elements on a microarray,
and protein expression levels are quantified by exposing the microarray to the
sample and detecting
the levels of protein bound to each array element (Lueking, A. et al. (1999)
Anal. Biochem. 270:103-
x5 11; Mendoze, L. G. et al. (1999) Biotechniques 27:778-88). Detection may be
performed by a variety
of methods known in the art, for example, by reacting the proteins in the
sample with a thiol- or amino-
reactive fluorescent compound and detecting the amount of fluorescence bound
at each array
element.
Toxicant signatures at the proteome level are also useful for toxicological
screening, and
3 o should be analyzed in parallel with toxicant signatures at the transcript
level. There is a poor
correlation between transcript and protein abundances for some proteins in
some tissues (Anderson,
N. L. and Seilhamer, J. (1997) Electrophoresis 18:533-537), so proteome
toxicant signatures may be
useful in the analysis of compounds which do not significantly affect the
transcript image, but which
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CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
alter the proteomic profile. In addition, the analysis of transcripts in body
fluids is difficult, due to rapid
degradation of mRNA, so proteomic profiling may be more reliable and
informative in such cases.
In another embodiment, the toxicity of a test compound is assessed by treating
a biological
sample containing proteins with the test compound. Proteins that are expressed
in the treated
s biological sample are separated so that the amount of each protein can be
quantified. The amount of
each protein is compared to the amount of the corresponding protein in an
untreated biological sample.
A difference in the amount of protein between the two samples is indicative of
a toxic response to the
test compound in the treated sample. Individual proteins are identified by
sequencing the amino acid
residues of the individual proteins and comparing these partial sequences to
the SPTM encoded by
to polynucleotides of the present invention.
In another embodiment, the toxicity of a test compound is assessed by treating
a biological
sample containing proteins with the test compound. Proteins from the
biological sample are incubated
with antibodies specific to the SPTM encoded by polynucleotides of the present
invention. The
amount of protein recognized by the antibodies is quantified. The amount of
protein in the treated
15 biological sample is compared with the amount in an untreated biological
sample. A difference in the
amount of protein between the two samples is indicative of a toxic response to
the test compound in
the treated sample.
Transcript images may be used to profile sptm expression in distinct tissue
types. This
process can be used to determine cell signaling activity in a particular
tissue type relative to this
a o activity in a different tissue type. Transcript images may be used to
generate a profile of sptm
expression characteristic of diseased issue. Transcript images of tissues
before and. after treatment
may be used for diagnostic purposes, to monitor the progression of disease,
and to monitor the efficacy
of drug treatments for diseases which affect cell signaling activity.
Transcript images of cell lines can be used to assess cell signaling activity
and/or to identify
a s cell lines that lack or misregulate this activity. Such cell lines may
then be treated with pharmaceutical
agents, and a transcript image following treatment may indicate the efficacy
of these agents in
restoring desired levels of this ,activity. A similar approach may be used to
assess the toxicity of
pharmaceutical agents as reflected by undesirable changes in cell signaling
activity. Candidate
pharmaceutical agents may be evaluated by comparing their associated
transcript images with those of
3 o pharmaceutical agents of known effectiveness.
Antisense Molecules
The polynucleotides of the present invention are useful in
antisense.technology. Antisense
42
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
technology or therapy relies on the modulation of expression of a target
protein through the specific
binding of an antisense sequence to a target sequence encoding the target
protein or directing its
expression. (See, e.g., Agrawal, S., ed. (1996) Antisense Therapeutics, Humana
Press Inc., Totawa
NJ; Alama, A. et al. (1997) Pharmacol. Res. 36(3):171-178; Crooke, S.T. (1997)
Adv. Pharmacol.
s 40:1-49; Sharma, H.W. and R. Narayanan (1995) Bioessays 17(12):1055-1063;
and Lavrosky, Y. et
al. (1997) Biochem. Mol. Med. 62(1):11-22.) An antisense sequence is a
polynucleotide sequence
capable of specifically hybridizing to at least a portion of the target
sequence. Antisense sequences
bind to cellular mRNA and/or genomic DNA, affecting translation and/or
transcription. Antisense
sequences can be DNA, RNA, or nucleic acid mimics and analogs. (See, e.g.,
Rossi, J.J. et al. (1991)
to Antisense Res. Dev. 1 (3):285-288; Lee, R. et al. (1998) Biochemistry
37(3):900-1010; Pardridge,
W.M, et al. (1995) Proc. Natl. Acad. Sci. USA 92(12):5592-5596; and Nielsen,
P. E. and Haaima, G.
(1997) Chem. Soc. Rev. 96:73-78.) Typically, the binding which results in
modulation of expression
occurs through hybridization or binding of complementary base pairs. Antisense
sequences can also
bind~to DNA duplexes through specific interactions in the major groove of the
double helix.
15 The polynucleotides of the present invention and fragments thereof can be
used as antisense
sequences to modify the expression of the polypeptide encoded by sptm. The
antisense sequences
can be produced ex vivo, such as by using any of the ABI nucleic acid
synthesizer series (Applied
Biosystems) or other automated systems known in the art. Antisense sequences
can also be produced .
biologically, such as by transforming an appropriate host cell with an
expression vector containing the
2 o sequence of interest. (See, e.g., Agrawal, supra.)
In therapeutic use, any gene delivery system suitable for introduction of the
antisense
sequences into appropriate target cells can be used. Antisense sequences can
be delivered
intracellularly in the form of an expression plasmid which, upon
transcription, produces a sequence
complementary to at least a portion of the cellular sequence encoding the
target protein. (See, e.g.,
25 Slater, J.E., et al. (1998) J. Allergy Clin. Immunol. 102(3):469-475; and
Scanlon, I~.J., et al. (1995)
9(13):1288-1296.) Antisense sequences can also be introduced intracellularly
through the use of viral
vectors, such as retrovirus and adeno-associated virus vectors. (See, e.g.,
Miller, A.D. (1990)Blood
76:271; Ausubel, F.M. et al. (1995) Current Protocols in Molecular Biolo~y,
John Wiley & Sons, New
York NY; Uckert~ W. and W. Walther (1994) Pharmacol. Ther. 63(3):323-347.)
Other gene delivery
3 o mechanisms include liposome-derived systems, artificial viral envelopes,
and other systems known in
the art. (See, e.g., Rossi, J.J. (1995) Br..Med. Bull. 51(1):217-225; Boado,
R.J. et al. (1998) J. Pharm.
Sci. 87(11):1308-1315; and Morris, M.C. et al. (1997) Nucleic Acids Res.
25(14):2730-2736.)
43
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WO 02/20756 PCT/USO1/27297
Expression
In order to express a biologically active SPTM, the nucleotide sequences
encoding SPTM or
fragments thereof may be inserted into an appropriate expression vector, i.e.,
a vector which contains
the necessary elements for transcriptional and translational control of the
inserted coding sequence in
s a suitable host. Methods which are well known to those skilled in the art
may be used to construct
expression vectors containing sequences encoding SPTM and appropriate
transcriptional and
translational control elements. These methods include in vitro recombinant DNA
techniques, synthetic
techniques, and in vivo genetic recombination. (See, e.g., Sambrook, supra,
Chapters 4, 8, 16, and 17;
and Ausubel, supra, Chapters 9, 10, 13, and 16.)
to A variety of expression vector/host systems may be utilized to contain and
express sequences
encoding SPTM. These include, but are not limited to, microorganisms such as
bacteria transformed
with recombinant bacteriophage, plasmid, or cosmid DNA expression vectors;
yeast transformed with
yeast expression vectors; insect cell systems infected with viral expression
vectors (e.g., baculovirus);
plant cell systems transformed with viral expression vectors (e.g.,
cauliflower mosaic virus, CaMV, or
15 tobacco mosaic virus, TMV) or with bacterial expression vectors (e.g., Ti
or pBR322 plasmids); or
animal (mammalian) cell systems. (See, e.g., Sambrook, supra; Ausubel, 1995,
supra, Van Heeke, G.
and S.M. Schuster (1989) J. Biol. Chem. 264:5503-5509; Bitter, G.A. et al.
(1987) Methods Enzymol.
153:516-544; Scorer, C.A. et al. (1994) Bio/Technology 12:181-184; Engelhard,
E.K. et al. (1994)
Proc. Natl. Acad. Sci. USA 91:3224-3227; Sandig, V. et al. (1996) Hum. Gene
Ther. 7:1937-1945;
2o Takamatsu, N. (1987) EMBO J. 6:307-311; Coruzzi, G. et al. (1984) EMBO J.
3:1671-1680; Brogue,
R. et al. (1984) Science 224:838-843; Winter, J. et al. (1991) Results Probl.
Cell Differ. 17:85-105;
The McGraw HillYearbook of Science and Technolo~y (1992) McGraw Hill, New York
NY, pp.
191-196; Logan, J. and T. Shenk (1984) Proc. Natl. Acad. Sci. USA 81:3655-
3659; and Harrington,
J.J. et al. (1997) Nat. Genet. 15:345-355.) Expression vectors derived from
retroviruses,
as adenoviruses, or herpes or vaccinia viruses, or from various bacterial
plasmids, may be used for
delivery of nucleotide sequences to the targeted organ, tissue, or cell
population. (See,.e.g., Di Nicola,
M. et al. (1998) Cancer Gen. Ther. 5(6):350-356; Yu, M. et al., (1993) Proc.
Natl. Acad. Sci. USA
90(13):6340-6344; Butler, R.M. et al. (1985) Nature 317(6040):813-815;
McGregor, D.P, et al. (1994)
Mol. Immunol: 31(3):219-226; and Verma, LM. and N. Somia (1997) Nature 389:239-
242.) The
s o invention is not limited by the host cell employed.
For long term production of recombinant proteins in mammalian systems, stable
expression of
SPTM in cell lines is preferred. For example, sequences encoding SPTM can be
transformed into cell
lines using expression vectors which may contain viral origins of replication
and/or endogenous
44
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
expression elements and a selectable marker gene on the same or on a separate
vector. Any number
of selection systems may be used to recover transformed cell lines. (See,
e.g., Wigler, M. et al.
(1977) Cell 11:223-232; Lowy, I. et al. (1980) Cell 22:817-823.; Wigler, M. et
al. (1980) Proc. Natl.
Acad. Sci. USA 77:3567-3570; Colbere-Garapin, F. et al. (1981) J. Mol. Biol.
150:1-14; Hartman, S.C.
s and R.C.Mulligan (1988) Proc. Natl. Acad. Sci. USA 85:8047-8051; Rhodes,
C.A. (1995) Methods
Mol. Biol. 55:121-131.)
Therapeutic Uses of sptm
The polynucleotides encoding SPTM of the invention may be used for somatic or
germline
to gene therapy. Gene therapy may be performed to (i) correct a genetic
deficiency (e.g., in the cases
of severe combined immunodeficiency (SCID)-Xl disease characterized by X-
linked inheritance
(Cavazzana-Calvo, M. et al. (2000) Science 288:669-672), severe combined
immunodeficiency
syndrome associated with an inherited adenosine deaminase (ADA) deficiency
(Blaese, R.M. et al.
(1995) Science 270:475-480; Bordignon, C. et al. (1995) Science 270:470-475),
cystic fibrosis (Zabner,
15 J. et al. (1993) Cell 75:207-216; Crystal, R.G. et al. (1995) Hum. Gene
Therapy 6:643-666; Crystal,
R.G. et al. (1995) Hum. Gene Therapy 6:667-703), thalassemias, familial
hypercholesterolemia, and
hemophilia resulting from Factor VIII or Factor IX deficiencies (Crystal, R.G.
(1995) Science
270:404-410; Verma~ LM. and Somia, N. (1997) Nature 389:239-242)), (ii)
express a conditionally
lethal gene product (e.g., in the case of cancers which result from
unregulated cell proliferation), or
20 (iii) express a protein which affords protection against intracellular
parasites (e.g., against human
retroviruses, such as human immunodeficiency virus (HIV) (Baltimore, D. (1988)
Nature 335:395-
396; Poeschla, E..et al. (1996) Proc. Natl. Acad. Sci. USA. 93:11395-11399),
hepatitis B or C virus
(HBV, HCV); fungal parasites, such as Candida alliicans and Paracoccidioides
brasiliensis; and
protozoan parasites such as Plasmodium falciparum and Tr<rpanosoma cruzi). In
the case where a
25 genetic deficiency in sptm expression or regulation causes disease, the
expression of sptm from an
appropriate population of transduced cells may alleviate the clinical
manifestations caused by the
genetic deficiency.
In a further embodiment of the invention, diseases or disorders caused by
deficiencies in sptm
are treated by constructing mammalian expression vectors comprising sptm and
introducing these
s o vectors by mechanical means into sptm-deficient cells. Mechanical transfer
technologies for use with
cells in vivo or ex vitro include (i) direct DNA microinjection into
individual cells, (ii) ballistic gold
particle delivery, (iii) liposome-mediated transfection, (iv) receptor-
mediated gene transfer, and (v) the
use of DNA transposons (Morgan, R.A. and Anderson, W.F. (1993) Annu. Rev.
Biochem. 62:191-
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
217; Ivics, Z. (1997) Cell 91:501-510; Boulay, J-L. and Recipon, H. (1998)
Curr. Opin. Biotechnol.
9:445-450).
Expression vectors that may be effective for the expression of sptm include,
but are not
limited to, the PCDNA 3.1, EPITAG, PRCCMV2, PREP, PVAX vectors (Invitrogen,
Carlsbad CA),
s PCMV-SCRIPT, PCMV-TAG, PEGSH/PERV (Stratagene, La Jolla CA), and PTET-OFF,
PTET-ON, PTRE2, PTRE2-LUC, PTK-HYG (Clontech, Palo Alto CA). The sptm of the
invention
may be expressed using (i) a constitutively active promoter, (e.g., from
cytomegalovirus (CMV), Rous
sarcoma virus (RSV), SV40 virus, thymidine kinase (TK), or (3-actin genes),
(ii) an inducible promoter
(e.g., the tetracycline-regulated promoter (Gossen, M. and Bujard, H. (1992)
Proc. Natl. Acad. Sci.
to U.S.A. 89:5547-5551; Gossen, M. et al., (1995) Science 268:1766-1769;
Rossi, F.M.V. and Blau,
H.M. (1998) Curr. Opin. Biotechnol. 9:451-456), commercially available in the
T-REX plasmid
(Invitrogen); the ecdysone-inducible promoter (available in the plasmids
PVGRXR and PIND;
Invitrogen); the FK506/rapamycin inducible promoter; or the RU486/mifepristone
inducible promoter
(Rossi, F.M.V. and Blau, H.M. suQra), or (iii) a tissue-specific promoter or
the native promoter of the
is endogenous gene encoding SPTM from a normal individual.
Commercially available liposome transformation kits (e.g., the PERFECT LIPID
TRANSFECTION KIT, available from Invitrogen) allow one with ordinary skill in
the art to deliver
polynucleotides to target cells in culture and require minimal effort to
optimize experimental .r
parameters. In the alternative, transformation is performed using the calcium
phosphate method
20 (Graham, F.L. and Eb, A.J. (1973) Virology 52:456-467), or by
electroporation (Neumann, E. et al.
(1982) EMBO J. 1:841-845). The introduction of DNA to primary cells requires
modification of these
standardized mammalian transfection protocols.
In another embodiment of the invention, diseases or disorders caused by
genetic defects with
respect to sptm expression are treated by constructing a retrovirus vector
consisting of (i) sptm under
25 the control of an independent promoter or the retrovirus long terminal
repeat (LTR) promoter, (ii)
appropriate RNA packaging signals, and (iii) a Rev-responsive element (RRE)
along with additional
retrovirus cis-acting RNA sequences and coding sequences required for
efficient vector propagation.
Retrovirus vectors (e.g., PFB and PFBNEO) are commercially available
(Stratagene) and are based
on published data (Riviere, I. et al. (1995) Proc. Natl. Acad. Sci. U.S.A.
92:6733-6737), incorporated
3 o by reference herein. The vector is propagated in an appropriate vector
producing cell fine (VPCL)
that expresses an envelope gene with a tropism for receptors on the target
cells or a promiscuous
envelope protein such as VSVg (Armentano, D. et al. (1987) J. Virol. 61:1647-
1650; Bender, M.A. et
al. (1987) J. Virol. 61:1639-1646; Adam, M.A. and Miller, A.D. (1988) J.
Virol. 62:3802-3806; Dull, T.
46
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
et al. (1998) J. Virol. 72:8463-8471; Zufferey, R. et al. (1998) J. Virol.
72:9873-9880). U.S. Patent
Number 5,910,434 to Rigg ("Method for obtaining retrovirus packaging cell
lines producing high
transducing efficiency retroviral supernatant") discloses a method for
obtaining retrovirus packaging
cell lines and is hereby incorporated by reference. Propagation of retrovirus
vectors, transduction of a
s population of cells (e.g., CD4+ T-cells), and the return of transduced cells
to a patient are procedures
well known to persons skilled in the art of gene therapy and have been well
documented (Ranga, U. et
al. (1997) J. Virol. 71:7020-7029; Bauer, G. et al. (1997) Blood 89:2259-2267;
Bonyhadi, M.L. (1997)
J. Virol. 71:4707-4716; Ranga, U. et al. (1998) Proc. Natl. Acad. Sci. U.S.A.
95:1201-1206; Su, L.
(1997) Blood 89:2283-2290).
to In the alternative, an adenovirus-based gene therapy delivery system is
used to deliver sptm to
cells which have one or more genetic abnormalities with respect to the
expression of sptm. The
construction and packaging of adenovirus-based vectors are well known to those
with ordinary skill in
the art. Replication defective adenovirus vectors have proven to be versatile
for importing genes
encoding immunoregulatory proteins into intact islets in the pancreas (Csete,
M.E. .et al. (1995)
15 Transplantation 27:263-268). Potentially useful adenoviral vectors are
described in U.S. Patent
Number 5,707,618 to Armentano ("Adenovirus vectors for gene therapy"), hereby
incorporated by
reference. For adenoviral vectors, see also Antinozzi, P.A: et al. (1999)
Annu. Rev. Nutr. 19:511-544:e
and Verma, LM. and Somia, N. (1997) Nature 18:389:239-242, both incorporated
by reference herein.'
In another alternative a herpes-based, gene therapy delivery system is used to
deliver sptm to
20 target cells which have one or more genetic abnormalities with respect to
the expression of sptm. The:
use of herpes simplex virus (HSV)-based vectors may be especially valuable for
introducing sptm to
cells of the central nervous system, for which HSV has a tropism. The
construction and packaging of
herpes-based vectors are well known to those with ordinary skill in the art. A
replication-competent
herpes simplex virus (HSV) type 1-based vector has been used to deliver a
reporter gene to the eyes
2s of primates (Liu, X. et al. (1999) Exp. Eye Res.169:385-395). The
construction of a HSV-1 virus
vector has also been disclosed in detail in U.S. Patent Number 5,804,413 to
DeLuca ("Herpes simplex
virus strains for gene transfer"), which is hereby incorporated by reference.
U.S. Patent Number
5,804,413 teaches the use of recombinant HSV d92 which consists of a genome
containing at least
one exogenous gene to be transferred to a cell under the control of the
appropriate promoter for
s o purposes including human gene therapy. Also taught by this patent are the
construction and use of
recombinant HSV strains deleted for ICP4, ICP27 and ICP22. For HSV vectors,
see also Goins, W.
F. et al. 1999 J. Virol. 73:519-532 and Xu, H. et al., (1994) Dev. Biol.
163:152-161, hereby
incorporated by reference. The manipulation of cloned herpesvirus sequences,
the generation of
47
CA 02419943 2003-02-20
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recombinant virus following the transfection of multiple plasmids containing
different segments of the
large herpesvirus genomes, the growth and propagation of herpesvirus, and the
infection of cells with
herpesvirus are techniques well known to those of ordinary skill in the art.
In another alternative, an alphavirus (positive, single-stranded RNA virus)
vector is used to
s deliver sptm to target cells. The biology of the prototypic alphavirus,
Semliki Forest Virus (SFV), has
been studied extensively and gene transfer vectors have been based on the SFV
genome (Garoff, H.
and Li, K-J. (1998) Curr. Opin. Biotech. 9:464-469). During alphavirus RNA
replication, a
subgenomic RNA is generated that normally encodes the viral capsid proteins.
This subgenomic RNA
replicates to higher levels than the full-length genomic RNA, resulting in the
overproduction of capsid
to proteins relative to the viral proteins with enzymatic activity (e.g.,
protease and polymerase).
Similarly, inserting sptm into the alphavirus genome in place of the capsid-
coding region results in the
production of a large number of sptm RNAs and the synthesis of high levels of
SPTM in vector
transduced cells. While alphavirus infection is typically associated with cell
lysis within a few days,
the ability to establish a persistent infection in hamster normal kidney cells
(BHK-21 ) with a variant of
is Sindbis virus (SIN) indicates that the lytic replication of alphaviruses
can be altered to suit the needs of
the gene therapy application (Dryga, S.A. et al. (1997) Virology 228:74-83).
The wide host range of
alphaviruses will allow the introduction of sptm into a variety of cell types.
The specific transduction .
of a subset of cells in a population may require the sorting of cells prior to
transduction. The methods
of manipulating infectious cDNA clones of alphaviruses~ performing alphavirus
cDNA and RNA
2 o transfections, and performing alphavirus infections, are well known to
those with ordinary skill in the
art.
Antibodies
Anti-SPTM antibodies may be used to analyze protein expression levels. Such
antibodies
as include, but are not limited to, polyclonal, monoclonal, chimeric, single
chain, and Fab fragments. For
descriptions of and protocols of antibody technologies, see, e.g., Pound J.D.
(1998) Immunochemical
Protocols, Humana Press, Totowa, NJ.
The amino acid sequence encoded by the sptm of the Sequence Listing may be
analyzed by
appropriate software (e.g., LASERGENE NAVIGATOR software, DNASTAR) to
determine
s o regions of high immunogenicity. The optimal sequences for immunization are
selected from the C-
terminus, the N-terminus, and those intervening, hydrophilic regions of the
polypeptide which are likely
to be exposed to the external environment when the polypeptide is in its
natural conformation.
Analysis used to select appropriate epitopes is also described by Ausubel
(1997, supra, Chapter 11.7).
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CA 02419943 2003-02-20
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Peptides used for antibody induction do not need to have biological activity;
however, they must be
antigenic. Peptides used to induce specific antibodies may-have an amino acid
sequence consisting of
at least five amino acids, preferably at least 10 amino acids, and most
preferably at least 15 amino
acids. A peptide which mimics an antigenic fragment of the natural polypeptide
may be fused with
s another protein such as keyhole limpet hemocyanin (KLH; Sigma, St. Louis MO)
for antibody
production. A peptide encompassing an antigenic region may be expressed from
an sptm, synthesized
as described above, or purified from human cells.
Procedures well known in the art may be used for the production of antibodies.
Various hosts
including mice, goats, and rabbits, may be immunized by injection with a
peptide. Depending on the
to host species, various adjuvants may be used to increase immunological
response.
In one procedure, peptides about 15 residues in length may be synthesized
using an ABI 431A
peptide synthesizer (Applied Biosystems) using fmoc-chemistry and coupled to
KI,H (Sigma) by
reaction with M-maleimidobenzoyl-N-hydroxysuccinimide ester (Ausubel, 1995,
supra). Rabbits are
immunized with the peptide-I~LH complex in complete Freund's adjuvant. The
resulting antisera are
15 tested for antipeptide activity by binding the peptide to plastic, blocking
with 1 % bovine serum albumin
(BSA), reacting with rabbit antisera; washing, and reacting with
radioiodinated goat anti-rabbit IgG.
Antisera with antipeptide activity are tested- for anti-SPTM activity using
protocols well known in the
art, including ELISA, radioimmunoassay (RIA), and immunoblotting.
In another procedure, isolated and purified peptide may be used to immunize
mice (about 100
ao ~g of peptide) or rabbits (about 1 mg of peptide). Subsequently, the
peptide is radioiodinated and used
to screen the immunized animals' B-lymphocytes for production of antipeptide
antibodies. Positive
cells are then used to produce hybridomas using standard techniques. About 20
mg of peptide is
sufficient for labeling and screening several thousand clones. Hybridomas of
interest are detected by
screening with radioiodinated peptide to identify those fusions producing
peptide-specific monoclonal
2s antibody. In a typical protocol, wells of a mufti-well plate (FAST, Becton-
Dickinson, Palo Alto, CA)
are coated with affinity-purified, specific rabbit-anti-mouse (or suitable
anti-species IgG) antibodies at
mg/ml. The coated wells are blocked with 1 % BSA and washed and exposed to
supernatants from
hybridomas. After incubation, the wells are exposed to radiolabeled peptide at
1 mg/ml.
Clones producing antibodies bind a quantity of labeled peptide that is
detectable above
a o background. Such clones are expanded and subjected to 2 cycles of cloning.
Cloned hybridomas are
injected into pristane-treated mice to produce ascites, and monoclonal
antibody is purified from the
ascitic fluid by affinity chromatography on protein A (Amersham Pharmacia
Biotech). Several
procedures for the production of monoclonal antibodies, including in vitro
production, are described in
49
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
Pound su ra). Monoclonal antibodies with antipeptide activity are tested for
anti-SPTM activity using
protocols well known in the art, including ELISA, RIA, and immunoblotting.
Antibody fragments containing specific binding sites for an epitope may also
be generated.
For example, such fragments include, but are not limited to, the F(ab~2
fragments produced by pepsin
s digestion of the antibody molecule, and the Fab fragments generated by
reducing the disulfide bridges
of the F.(ab~2 fragments. Alternatively, construction of Fab expression
libraries in filamentous
bacteriophage allows rapid and easy identification of monoclonal fragments
with desired specificity
(Pound, supra, Chaps. 45-47). Antibodies generated against polypeptide encoded
by sptm can be used
to purify and characterize full-length SPTM protein and its activity, binding
partners, etc.
Assays Using Antibodies
Anti-SPTM antibodies may be used in assays to quantify the amount of SPTM
found in a
particular human cell. Such assays include methods utilizing the antibody and
a label'to detect
expression level under normal or disease conditions. The peptides and
antibodies of the invention may
be used with or without modification or labeled by joining them, either
covalently or noncovalently,
with a reporter molecule.
Protocols for detecting and measuring protein expression using either
polyclonal or monoclonal
antibodies are well known in the art. Examples include ELISA, RIA, and
fluorescent activated cell
sorting (FACS). Such immunoassays typically involve the formation of complexes
between the SPTM
2 o and its specific antibody and the measurement of such complexes. These and
other assays are
described in Pound su ra).
Without further elaboration, it is believed that one skilled in the art can,
using the preceding
description, utilize the present invention to its fullest extent: The
following preferred specific
embodiments are, therefore, to be construed as merely illustrative, and not
liinitative of the remainder
of the disclosure in any way whatsoever.
The disclosures of all patents, applications, and publications mentioned above
and
below, including U.S: Ser. No. 60/230,517, U.S. Ser. No. 60/230,599, U.S. Ser.
No. 60/230,514, U.S.
U.S. Ser. No. 60/230,988, U.S. Ser. No. 60/230,518, U.S. Ser. No. 60/230,515,
U.S. Ser. No.
60/229,751, U.S. Ser. No. 60/230,016, U.S. Ser. No. 60/230,610, U.S. Ser. No.
60/229,749, U.S. Sex.
3o No. 60/229,750, U.S. Ser. No. 60/230,597, U.S. Ser. No. 60/230,505, U.S.
Ser. No. 60/231,163, U.S.
Ser. No. 60/229,747, U.S. Ser. No. 60/229,748, U.S. Ser. No. 60/230,583, U.S.
Ser. No. 60/230,519,
U.S. Ser. No. 60/230,595, U.S. Ser. No. 60/230,896, U.S. Ser. No. 60/230,990,
U.S. Ser. No.
60/230,865, U.S. Ser. No. 60/230,989, U.S. Ser. No. 60/230,897, U.S. Ser. No.
60/231,832, U.S. Sex.
CA 02419943 2003-02-20
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No. 60/230,596, U.S. Ser. No. 60/230,864, and U.S. Ser. No. 60/230,951 are
hereby expressly
incorporated by reference.
EXAMPLES
s I. Construction of cDNA Libraries
RNA was purchased from CLONTECH Laboratories, Inc. (Palo Alto CA) or isolated
from
various tissues. Some tissues were homogenized and lysed in guanidinium
isothiocyanate, while others
were homogenized and lysed in phenol or in a suitable mixture of denaturants,
such as TRIZOL (Life
Technologies), a monophasic solution of phenol and guanidine isothiocyanate.
The resulting lysates
to were centrifuged over CsCI cushions or extracted with chloroform. RNA was
precipitated with either
isopropanol or sodium acetate and ethanol, or by other routine methods.
Phenol extraction and precipitation of RNA were repeated as necessary to
increase RNA
purity. In most cases, RNA was treated with DNase. For most libraries,
poly(A+) RNA was isolated
using oligo d(T)-coupled paramagnetic particles (Promega Corporation
(Promega), Madison WI),
. is OLIGOTEX latex particles (QIAGEN, Inc. (QIAGEN), Valencia CA), or an
OLIGOTEX mRNA
purification kit (QIAGEN). Alternatively, RNA was isolated directly from
tissue lysates using other
RNA isolation kits, e.g., the POLY(A)PURE mRNA purification kit (Ambion, Inc.,
Austin TX).
In some cases, Stratagene was provided with RNA and constructed the
corresponding cDNA
libraries. 'Otherwise, cDNA was synthesized arid cDNA libraries were
constructed with the
2 o UNIZAP vector system (Stratagene Cloning Systems, Inc. (Stratagene), La
Jolla CA) or
SUPERSCRIPT plasmid system (Life Technologies), using the recommended
procedures or similar
methods known in.the art. (See, e.g., Ausubel, 1997, supra, Chapters 5.1
through 6.6.) Reverse
transcription was initiated using oligo d(T) or random primers. Synthetic
oligonucleotide adapters
were ligated to double stranded cDNA, and the cDNA was digested with the
appropriate restriction
2s enzyme or enzymes. For most libraries, the cDNA was size-selected (300-1000
bp) using
SEPHACRYL 51000, SEPHAROSE CL2B, or SEPHAROSE CL4B column chromatography
(Amersham Pharmacia Biotech) or preparative agarose gel electrophoresis. cDNAs
were ligated into
compatible restriction enzyme sites of the polylinker of a suitable plasmid,
e.g., PBLUESCRIPT
plasmid (Stratagene), PSPORT1 plasmid (Life Technologies), PCDNA2.1 plasmid
(Invitrogen,
s o Carlsbad CA), PBK-CMV plasmid (Stratagene), or pINCY (Incyte Genomics,
Palo Alto CA), or
derivatives thereof. Recombinant plasmids were transformed into competent E.
coli. cells including
XLl-Blue, XLl-BIueMRF, or SOLR from Stratagene or DHSa, DH10B, or ElectroMAX
DH10B
from Life Technologies. _
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II. Isolation of cDNA Clones
Plasmids were recovered from host cells by in vivo excision using the UNIZAP
vector system
(Stratagene) or by cell lysis. Plasmids were purified using at least one of
the following: the Magic or
WIZARD Minipreps DNA purification system (Promega); the AGTC Miniprep
purification kit (Edge
BioSystems, Gaithersburg MD); and the QIAWELL 8, QIAWELL 8 Plus, and QIAWELL 8
Ultra
plasmid purification systems or the R.E.A.L. PREP 96 plasmid purification kit
(QIAGEN). Following
precipitation, plasmids were resuspended in 0.1 ml of distilled water and
stored, with or without
lyophilization, at 4°C.
Alternatively, plasmid DNA was amplified from host cell lysates using direct
link PCR in a
to high-throughput format. (Rao, V.B. (1994) Anal. Biochem. 216:1-14.) Host
cell lysis and thermal
cycling steps were carried out in a single reaction mixture. Samples were
processed and stored in
384-well plates, and the concentration of amplified plasmid DNA was quantified
fluorometrically using
PICOGREEN dye (Molecular Probes, Inc. (Molecular Probes), Eugene OR) and a
FLUOROSKAN
II fluorescence scanner (Labsystems Oy, Helsinki, Finland).
III. Sequencing and Analysis
cDNA sequencing reactions were processed using standard methods or high-
throughput
instrumentation such as the ABI CATALYST 800 thermal cycler (Applied
Biosystems) or the PTC- '
200 thermal cycler (MJ Research) in conjunction with the HYDRA microdispenser
(Robbins
2 o Scientific Corp., Sunnyvale CA) or the MICROLAB 2200 liquid transfer
system (Hamilton). cDNA
sequencing reactions were prepared using reagents provided by Amersham
Pharmacia Biotech or
supplied in ABI sequencing kits such as the ABI PRISM BIGDYE Terminator cycle
sequencing
ready reaction kit (Applied Biosystems). Electrophoretic separation of cDNA
sequencing reactions
and detection of labeled polynucleotides were carried out using the MEGABACE
1000 DNA
sequencing system (Molecular Dynamics); the ABI PRISM 373 or 377 sequencing
system (Applied
Biosystems) in conjunction with standard ABI protocols and base calling
software; or other sequence
analysis systems known in the art. Reading frames within the cDNA sequences
were identified using
standard methods (reviewed in Ausubel, 1997, supra, Chapter 7.7). Some of the
cDNA sequences
were selected for extension using the techniques disclosed in Example VIII.
IV. Assembly and Analysis of Sequences
Component sequences from chromatograms were subject to PHRED analysis and
assigned a
quality score. The sequences having at least a required quality score were
subject to various pre-
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processing editing pathways to eliminate, e.g., low quality 3' ends, vector
and linker sequences, polyA
tails, Alu repeats, mitochondrial and ribosomal sequences, bacterial
contamination sequences, and
sequences smaller than 50 base pairs. In particular, low-information sequences
and repetitive
elements (e.g., dinucleotide repeats, Alu repeats, etc.) were replaced by
"n's", or masked, to prevent
s spurious matches.
Processed sequences were then subject to assembly procedures in which the
sequences were
assigned to gene bins (bins). Each sequence could only belong to one bin.
Sequences in each gene
bin were assembled to produce consensus sequences (templates). Subsequent new
sequences were
added to existing bins using BLASTn (v.1.4 WashLl) and CROSSHATCH. Candidate
pairs were
to identified as all BLAST hits having a quality score greater than or equal
to 150. Alignments of at least
82% local identity were accepted into the bin. The component sequences from
each bin were
assembled using a version of PHRAP. Bins with several overlapping component
sequences were
assembled using DEEP PHRAP. The orientation (sense or antisense) of each
assembled template
was determined based on the number and orientation of its component sequences
Template
15 sequences as disclosed in the sequence listing correspond to sense strand
sequences (the "forward"
reading frames), to the best determination. The complementary (antisense)
strands are inherently
disclosed herein. The component sequences which were used to assemble each
template consensus
sequence are listed in Table 3 along with their positions along the template
nucleotide sequences:
Bins were compared against each other and those having local similarity of at
least 82% were
ao combined and reassembled. Reassembled bins having templates of insufficient
overlap (less than 95%
local identity) were re-split. Assembled templates were also subject to
analysis by
STITCHER/EXON MAPPER algorithms which analyze the probabilities of the
presence of splice
variants, alternatively spliced exons, splice junctions, differential
expression of alternative spliced
genes across tissue types or disease states, etc. These resulting bins were
subject to several rounds
as of the above assembly procedures.
Once gene bins were generated based upon sequence alignments, bins were clone
joined
based upon clone information. If the 5' sequence of one clone was present in
one bin and the 3'
sequence from the same clone was present in a different bin, it was likely
that the two bins actually
belonged together in a single bin. The resulting combined bins underwent
assembly procedures to
3 o regenerate the consensus sequences.
The final assembled.templates were subsequently annotated using the following
procedure.
Template sequences were analyzed using BLASTn (v2.0, NCBn versus gbpri
(GenBank version
124). "Hits" were defined as an exact match having from 95% local identity
over 200 base pairs
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through 100% local identity over 100 base pairs, or a homolog match having an
E-value, i.e. a
probability score, of < 1 x 10-8. The hits were subject to frameshift FASTx
versus GENPEPT
(GenBank version 124). (See Table 6). In this analysis, a homolog match was
defined as having an
E-value of <_ 1 x 10-8. The assembly method used above was described in
"System and Methods for
s Analyzing Biomolecular Sequences," U.S.S.N. 09/276,534, filed March 25,
1999, and the LIFESEQ
Gold user manual (Incyte) both incorporated by reference herein.
Following assembly, template sequences were subjected to motif, BLAST, and
functional
analyses, and categorized in protein hierarchies using methods described in,
e.g., "Database System
Employing Protein Function Hierarchies for Viewing Biomolecular Sequence
Data," U.S.S:N.
l0 08/812,290, filed March 6, 1997; "Relational Database for Storing
Biomolecule Information," U.S.S.N.
08/947,845, filed October 9, 1997; "Project-Based Full-Length Biomolecular
Sequence Database,"
U.S.S.N. 08/811,758, filed March 6, 1997; and "Relational Database and System
for Storing
Information Relating to Biomolecular Sequences," U.S.S.N. 09/034,807, filed
March 4, 1998, all of
which are incorporated by reference herein.
15 The template sequences were further analyzed by translating each template
in all three
forward reading frames and searching each translation against the Pfam
database of hidden Markov
model-based protein families and domains using the HMMER software package
(available to the
public from Washington University School of Medicine, St. Louis MO). (See also
World Wide Web
site http://pfam.wustl.edu/ for detailed- descriptions of Pfam protein domains
and families.)
2 o Additionally, the template sequences were translated in all three forward
reading frames, and
each translation was searched against hidden Markov models for signal peptides
using the HMMER
software~package. Construction of hidden Markov models and their usage in
sequence analysis has
been described. (See, for example, Eddy, S.R. (1996) Curr. Opin. Str. Biol.
6:361-365.) Only those
signal peptide hits with a cutoff score of 11 bits or.greater are reported. A
cutoff score of 11 bits or
2s greater corresponds to at least about 91-94% true-positives in signal
peptide prediction. Template
sequences were also translated in all three forward reading frames, and each
translation was
searched against TMAP,, a program that uses weight matrices to delineate
transmembrane segments
on protein sequences. and determine orientation, with respect to the cell
cytosol (Persson, B. and
Argot, P. (1994) J. Mol. Biol. 237:182-192, and Persson, B. and Argos, P.
(1996) Protein Sci. 5:363-
3 0 371.) Regions of templates which, when translated, contain similarity to
signal peptide or
transmembrane consensus sequences are reported in Table 2.
Template sequences are further analyzed using the bioinformatics tools listed
in Table 6, or
using sequence analysis software known in the art such as MACDNASIS PRO
software (Hitachi
54
CA 02419943 2003-02-20
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Software Engineering, South San Francisco CA) and LASERGENE software
(DNASTAR).
Template sequences may be further quexied against public databases such as the
GenBank rodent,
mammalian, vertebrate, prokaryote, and eukaryote databases.
The template sequences were translated to derive the corresponding longest
open reading
s frame as presented by the polypeptide sequences as reported in Table 1.
Alternatively, a polypeptide
of the invention may begin at any of the methionine residues within the full
length translated
polypeptide. Polypeptide sequences were subsequently analyzed by querying
against the GenBank
protein database' (GENPEPT, (GenBank version 124)). Full length polynucleotide
sequences are also
analyzed using MACDNASIS PRO software (Hitachi Software Engineering, South San
Francisco
1 o CA) and LASERGENE software (DNASTAR). PolynucIeotide and polypeptide
sequence alignments
are generated using default parameters specified by the CLUSTAL algorithm as
incorporated into the
MEGALIGN muItisequence alignment program (DNASTAR), which also calculates the
percent
identity between aligned sequences.
Table 5 shows sequences with homology to the polypeptides of the invention as
identified by
15 BLAST analysis against the GenBank protein (GENPEPT) database. Column 1
shows the
polypeptide sequence identification number (SEQ >D NO:) for the polypeptide
segments of the
invention. Column 2 shows the reading frame used in the translation of the
polynueleotide sequences
encoding the polypeptide segments. Column 3 shows the length of the translated
polypeptide
segments. Columns 4 and 5 show the start and stop nucleotide positions of the
polynucleotide
2 o sequences encoding the polypeptide segments. Column 6 shows the GenBank
identification number
(GI Number) of the nearest GenBank homolog. Column 7 shows the probability
score for the match
between each polypeptide and its GenBank homolog. Column 8 shows the
annotation of the GenBank
homolog.
2 s V. Analysis of Polynacleotide Expression
Northern analysis is a laboratory technique used to detect the presence of a
transcxipt of a
gene and involves the hybridization of a labeled nucleotide sequence to a
membrane on which RNAs
from a particular cell type or tissue have been bound. (See, e.g., Sambrook,
su~a, ch. 7; Ausubel,
1995, supra, ch. 4 and 16.)
3 o Analogous computer techniques applying BLAST were used to search for
identical or related
molecules in cDNA databases such as GenBank or LIFESEQ (Incyte Genomics)~.
This analysis is
much faster than multiple membrane-based hybridizations. In addition, the
sensitivity of the computer
search can be modified to determine whether any particular match is
categorized as exact or similar.
CA 02419943 2003-02-20
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The basis of the search is the product score, which is defined as:
BLAST Score x Percent IdentiW
Xminimum {length(Seq. 1), length(Seq. 2)}
5
The product score takes into account both the degree of similarity between two
sequences and the
length of the sequence match. The product score is a normalized value between
0 and 100, and is
calculated as follows: the BLAST score is multiplied by the percent nucleotide
identity and the
product is divided by (5 times the length of the shorter of the two
sequences). The BLAST score is
t o calculated by assigning a score of +5 for every base that matches in a
high-scoring segment pair
(HSP), and -4 for every mismatch. Two sequences may share more than one HSP
(separated by
gaps). If there is more than one HSP, then the pair with the highest BLAST
score is used to calculate
the product score. . The product score represents a balance between fractional
overlap and quality in a
BLAST alignment. For example, a product score of 100 is produced only for 100%
identity over the
entire length of the shorter of the two sequences being compared. A product
score of 70 is produced
either by 100% identity and 70% overlap at one end, or by 88% identity and
100% overlap at the
other. A product score of 50 is produced either by 100% identity and 50%
overlap at one end, or 79%
identity and 100% overlap.
Alternatively, polynucleotide sequences encoding SPTM are analyzed with
respect to the
2 0 tissue sources from which they were derived. Polynucleotide sequences
encoding SPTM were
assembled, at least in part, withoverlapping Incyte cDNA sequences. Each cDNA
sequence is
derived from a cDNA library constructed from a human tissue. Each human tissue
is classified into
one of the following organ/tissue categories: cardiovascular system;
connective tissue; digestive
system; embryonic structures; endocrine system; exocrine glands; genitalia,
female; genitalia, male;
germ cells; heroic and immune system; liver; musculoskeletal system; nervous
system; pancreas;
respiratory system; sense organs; skin; stomatognathic system;
unclassified/mixed; or urinary tract.
The number of libraries in each category for each polynucleotide sequence
encoding SPTM is counted
and divided by the total number of libraries across all categories for each
polynucleotide sequence
encoding SPTM. Similarly, each human tissue is classified into one of the
following disease/condition
s o categories: cancer, cell line, developmental, inflammation, neurological,
trauma, cardiovascular, pooled,
and other, and the number of libraries in each category for each
polynucleotide sequence encoding
SPTM is counted and divided by the total number of libraries across all
categories for each
polynucleotide sequence encoding SPTM. The resulting percentages reflect the
tissue-specific and
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WO 02/20756 PCT/USO1/27297
disease-specific expression of cDNA encoding SPTM. Percentage values of tissue-
specific
expression are reported in Table 4. cDNA sequences and cDNA library/tissue
information are found
in the LIFESEQ GOLD database (Incyte Genomics, Palo Alto CA).
s VI. Tissue Distribution Profiling
A tissue distribution profile is determined for each template by compiling the
cDNA library
tissue classifications of its component cDNA sequences. Each component
sequence, is derived from
a cDNA library constructed from a human tissue. Each human tissue is
classified into one of the
following categories: cardiovascular system; connective tissue; digestive
system; embryonic
to structures; endocrine system; exocrine glands; genitalia, female;
genitalia, male; germ cells; heroic and
immune system; liver; musculoskeletal system; nervous system; pancreas;
respiratory system; sense
organs; skin; stomatognathic system; unclassified/mixed; or urinary tract.
Template sequences,
component sequences, and cDNA library/tissue information are found in the
LIFESEQ GOLD
database (Incyte Genomics, Palo Alto CA).
15 Table 4 shows the tissue distribution profile for the templates of the
invention. For each
template, the three most frequently observed tissue categories are shown in
column 2, .along with the
percentage of component sequences belonging to each category. Only tissue
categories with
percentage values of > 10% are shown. A tissue distribution of "widely
distributed'' in column 2
indicates percentage values of <10% in all tissue categories.
VII. Transcript Image Analysis
Transcript 'images are generated as described in Seilhamer et al.,
"Comparative Gene
Transcript Analysis," U.S. Patent Number 5,840,484, incorporated herein by
reference.
a s VIII. Extension of Polynucleotide Sequences and Isolation of a Full-length
cDNA
Oligonucleotide primers designed using an sptm of the Sequence Listing are
used to extend
the nucleic acid sequence. One primer is synthesized to initiate 5' extension
of the template, and the
other primer, to initiate 3' extension of the template. The initial primers
may be designed using OLIGO
4.06 software (National Biosciences, Inc. (National Biosciences), Plymouth
MN), or another
3 o appropriate program, to be about 22 to 30 nucleotides in length, to have a
GC content of about 50% or
more, and to anneal to the target sequence at temperatures of about
68°C to about 72°C. Any
stretch of nucleotides which would result in hairpin structures and primer-
primer dimerizations are
avoided. Selected human cDNA libraries are used to extend the sequence. If
more than one
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WO 02/20756 PCT/USO1/27297
extension is necessary or desired, additional or nested sets of primers are
designed.
High fidelity amplification is obtained by PCR using methods well known in the
art. PCR is
performed in 96-well plates using the PTC-200 thermal cycler (MJ Research).
The reaction mix
contains DNA template, 200 nmol of each primer, reaction buffer containing
Mg2+, (NH4)ZS04, and 13-
mercaptoethanol, Taq DNA polymerise (Amersham Pharmacia Biotech), ELONGASE
enzyme (Life
Technologies), and Pfu DNA polymerise (Stratagene), with the following
parameters for primer pair
PCI A and PCI B: Step 1: 94°C, 3 min; Step 2: 94°C, 15 sec; Step
3: 60°C, 1 min; Step 4: 68°C, 2
min; Step 5: Steps 2, 3, and 4 repeated 20 times; Step 6: 68°C, 5 min;
Step 7: storage at 4°C. In the
alternative, the parameters for primer pair T7 and SK+ are as follows: Step 1:
94°C, 3 min; Step 2:
94 ° C, 15 sec; Step 3: 57 ° C, 1 min; Step 4: 68 ° C, 2
min; Step 5: Steps 2, 3, and 4 repeated 20 times;
Step 6: 68°C, 5 min; Step 7: storage at 4°C.
The concentration of DNA in each well is determined by dispensing 100 ~1
PICOGREEN
quantitation reagent (0.25% (v/v); Molecular Probes) dissolved in 1X Tris-EDTA
(TE) and 0.5 ~1 of
undiluted PCR product into each well of an opaque fluorimeter plate (Corning
Incorporated (Corning),
Corning NY), allowing the DNA to bind to the reagent. The plate is scanned in
a FLUOROSKAN II
(Labsystems Oy) to measure the fluorescence of the sample and to quantify the
concentration of
DNA. A 5 ~l to 10 ~l aliquot of the reaction mixture is analyzed by
electrophoresis on a 1 % agarose
mini-gel to determine which reactions are successful in extending the
sequence.
The extended nucleotides are desalted and concentrated, transferred to 384-
well plates,
2 o digested with CviJI cholera virus endonuclease (Molecular Biology
Research, Madison WI), and
sonicated or sheared prior to religation into pUC 18 vector (Amersham
Pharmacia Biotech). For
shotgun sequencing, the digested nucleotides are separated on low
concentration (0.6 to 0.8%)
agarose gels, fragments are excised, and agar digested with AGAR ACE
(Promega). Extended
clones are religated using T4 lipase (New England Biolabs, Inc., Beverly MA)
into pUC 18 vector
(Amersham Pharmacia Biotech), treated with Pfu DNA polymerise (Stratagene) to
fill-in restriction
site overhangs, and transfected into competent E. coli cells. Transformed
cells are selected on
antibiotic-containing media, individual colonies are picked and cultured
overnight at 37°C in 384-well
plates in LB/2x carbenicillin liquid media.
The cells are lysed, and DNA is amplified by PCR using Taq DNA polymerise
(Amersham
s o Pharmacia Biotech) and Pfu DNA polymerise (Stratagene) with the following
parameters: Step 1:
94°C, 3 min; Step 2: 94°C, 15 sec; Step 3: 60°C, 1 min;
Step 4: 72°C, 2 min; Step 5: steps 2, 3, and 4
repeated 29 times; Step 6: 7.2°C, 5 min; Step 7: storage at 4°C.
DNA is quantified by PICOGREEN
reagent (Molecular Probes) as described above. Samples with low DNA recoveries
are reamplified
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using the same conditions as described above. Samples are diluted with 20%
dimethysulfoxide (1:2,
vlv), and sequenced using DYENAMIC energy transfer sequencing primers and the
DYENAMIC
DIRECT kit (Amersham Pharmacia Biotech) or the ABI PRISM BIGDYE Terminator
cycle
sequencing ready reaction kit (Applied Biosystems).
In like manner, the sptm is used to obtain regulatory sequences (promoters,
introns, and
enhancers) using the procedure above, oligonucleotides designed for such
extension, and an
appropriate genomic library.
IX. Labeling of Probes and Southern Hybridization Analyses
to Hybridization probes derived from the sptm of the Sequence Listing are
employed for
screening cDNAs, mRNAs, or genomic DNA. The labeling of probe nucleotides
between 100 and
1000 nucleotides in length is specifically described, but essentially the same
procedure may be used
with larger cDNA fragments. Probe sequences are labeled at room temperature
for 30 minutes using
a T4 polynucleotide kinase, y32P-ATP, and O.SX One-Phor-All Plus (Amersham
Pharmacia Biotech)
15 buffer and purified using a ProbeQuant G-50 Microcolumn (Amersham Pharmacia
Biotech).. The
probe mixture is diluted to 10' dpm/~g/ml hybridization buffer and used in a
typical membrane-based
hybridization analysis.
The DNA is digested with a restriction endonuclease such as Eco RV and is
electrophoresed
through a 0.7% agarose gel: The DNA fragments are transferred from the agarose
to nylon
20 membrane (NYTRAN Plus, Schleicher & Schuell, Inc., Keene NH) using
procedures specified by the
manufacturer of the membrane. Prehybridization is carried out for three or
more hours at 68 °C, and
hybridization is carried out overnight at 68 °C. To remove non-specific
signals, blots are sequentially
washed at room temperature under increasingly stringent conditions, up to O.lx
saline sodium citrate
(SSC) and 0.5% sodium dodecyl sulfate. After the blots are placed in a
PHOSPHORIMAGER .
as cassette (Molecular Dynamics) or are exposed to autoradiography film,
hybridization patterns of
standard and experimental lanes are compared. Essentially the same procedure
is employed when
screening RNA.
X. Chromosome Mapping of sptm
s o The cDNA sequences which were used to assemble SEQ ID NO:1-184 are
compared with
sequences from the Incyte LIFESEQ database and public domain databases using
BLAST and other
implementations of the Smith-Waterman algorithm. Sequences from these
databases that match SEQ
B7 N0:1-184 are assembled into clusters of contiguous and overlapping
sequences using assembly
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algorithms such as PHRAP (Table 6). Radiation hybrid and genetic mapping data
available from
public resources such as the Stanford Human Genome Center (SHGC), Whitehead
Institute for
Genome Research (WIGR)~ and Genethon are used to determine if any of the
clustered sequences
have been previously mapped. Inclusion of a mapped sequence in a cluster will
result in the
s assignment of all sequences of that cluster, including its particular SEQ ID
NO:, to that map location.
The genetic map locations of SEQ ID NO:l-184 are described as ranges, or
intervals, of human
chromosomes. The map position of an interval, in centiMorgans, is measured
relative to the terminus
of the chromosome's p-arm. (The centiMorgan (cM) is a unit of measurement
based on
recombination frequencies between chromosomal markers. On average, 1 cM is
roughly equivalent to
so 1 megabase (Mb) of DNA in humans, although this can vary widely due tohot
and cold spots of
recombination.) The cM distances are based on genetic markers mapped by
Genethon which provide
boundaries for radiation hybrid markers whose sequences were included in each
of the clusters.
XI. Microarray Analysis
15 Probe Preparation from Tissue or Cell Samples
Total RNA is isolated from tissue samples using the guanidinium thiocyanate
method arid
polyA+ RNA is purified using the oligo (dT) cellulose method. Each polyA+ RNA
sample is reverse
transcribed using MMLV reverse-transcriptase, 0.05 pg/~1 oligo-dT primer
(2lmer), 1X first strand
buffer, 0.03 units/~1 RNase inhibitor; 500 ~M dATP, 500 ~M dGTP, 500 ~M dTTP,
40 ~M dCTP, 40
2o pM dCTP-Cy3 (BDS) or dCTP-Cy5 (Amersham Pharmacia Biotech). The reverse
transcription
reaction is performed in a 25 ml volume containing 200 ng polyA+ RNA with
GEMBRIGHT kits
(Incyte). Specific control polyA+ RNAs are synthesized by in vitro
transcription from non-coding
yeast genomic DNA (W. Lei, unpubfished). As quantitative controls, the control
mRNAs at 0.002 ng,
0.02 ng, 0.2 ng, and 2 ng are diluted into reverse transcription reaction at
ratios of 1:100,000, '1:10,000,
25 1:1000, 1:100 (w/w) to sample mRNA respectively. The control mRNAs are
diluted into reverse
transcription reaction at ratios of 1:3, 3:1, 1:10, 10:1, 1:25, 25:1 (w/w) to
sample mRNA differential
expression patterns. After incubation at 37° C for 2 hr, each reaction
sample (one with Cy3 and
another with Cy5 labeling) is treated with 2.5 ml of 0.5M sodium hydroxide and
incubated for 20
minutes at 85° C to the stop the reaction and degrade the RNA. Probes
are purified using two
s o successive CHROMA SPIN 30 gel filtration spin columns (CLONTECH
Laboratories, Inc.
(CLONTECH), Palo Alto CA) and after combining, both reaction samples are
ethanol precipitated
using 1 ml of glycogen (1 mg/ml), 60 ml sodium acetate, and 300 ml of 100%
ethanol. The probe is
then dried to completion using a SpeedVAC (Savant Instruments Inc., Holbrook
NY) and resuspended
CA 02419943 2003-02-20
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in 14 ~1 SX SSC/0.2% SDS.
Microarray Preparation
Sequences of the present invention are used to generate array elements. Each
array element
s is amplified from bacterial cells containing vectors with cloned cDNA
inserts. PCR amplification uses
primers complementary to the vector sequences flanking the cDNA insert. Array
elements are
amplified in thirty cycles of PCR from an initial quantity of 1-2 ng to a
final quantity greater than 5 ~ g.
Amplified array elements are then purified using SEPHACRYL-400 (Amersham
Pharmacia Biotech).
Purified array elements are immobilized on polymer-coated glass slides. Glass
microscope
1 o slides (Corning) are cleaned by ultrasound in 0.1 % SDS and acetone, with
extensive distilled water
washes between and after treatments. Glass slides are etched in 4%
hydrofluoric acid (VWR
Scientific Products Corporation (VWR), West Chester, PA), washed extensively
in distilled water, and
coated with 0.05% aminopropyl silane (Sigma) in 95% ethanol. Coated slides are
cured in a 110°C
oven.
15 Array elements are applied to the coated glass substrate using a procedure
described in US
Patent No. 5,807,522, incorporated herein by reference. 1 p1 of the array
element DNA,. at an average
concentration of 100 ng/~1, is loaded into the open capillary printing element
by a high-speed robotic
apparatus. The apparatus then deposits about 5 n1 of array element sample per
slide.
Microarrays are UV-crosslinked using a STRATALINI~ER UV-crosslinker
(Stratagene). .
2 o Microarrays are washed at room temperature once in 0.2% SDS and three
times in distilled water..
Non-specific binding sites are blocked by incubation of microarrays in 0.2%
casein in phosphate
buffered, saline (PBS) (Tropix, Inc., Bedford, MA) for 30 minutes at
60° C followed by washes in
0.2% SDS and distilled water as before.
2 s Hybridization
Hybridization reactions contain 9 ~ul of probe mixture consisting of 0.2 yg
each of Cy3 and Cy5
labeled cDNA synthesis products in SX SSC, 0.2% SDS hybridization buffer. The
probe mixture is
heated to 65° C for 5 minutes and is aliquoted onto the microarray
surface and covered with an 1.8
cm2 coverslip. The arrays are transferred to a waterproof chamber having a
cavity just slightly larger
s o than a microscope slide. The chamber is kept at 100% humidity internally
by the addition of 140 p l of
Sx SSC in a corner of the chamber. The chamber containing the arrays is
incubated for about 6.5
hours at 60° C. The arrays are washed for 10 min at 45° C in a
first wash buffer (IX SSC, 0.1 %
SDS), three times for 10 minutes each at 45° C in a second wash buffer
(0.1X SSC), and dried. .
61
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
Detection
Reporter-labeled hybridization complexes are detected with a microscope
equipped with an
Innova 70 mixed gas 10 W laser (Coherent, Inc., Santa Clara CA) capable of
generating spectral lines
at 488 nm for excitation of Cy3 and at 632 nm for excitation of CyS. The
excitation laser light is
s focused on the array using a 20X microscope objective (Nikon, Inc., Melville
NY). The slide
containing the array is paced on a computer-controlled X-Y stage on the
microscope and raster-
scanned past the objective. The 1.8 cm x 1.8 cm array used in the present
example is scanned with a
resolution of 20 micrometers.
In two separate scans, a mixed gas multiline laser excites the two
fluorophores sequentially.
to Emitted light is split, based on wavelength, into two photomultiplier tube
detectors (PMT 81477,
Hamamatsu Photonics Systems, Bridgewater NJ) corresponding to the two
fluorophores. Appropriate
filters positioned between the array and the photomultiplier tubes are used to
filter the signals. The
emission maxima of the fluorophores used are 565 nm for Cy3 and 650 nm for
CyS. Each array is
typically scanned twice, one scan per fluorophore using the appropriate
filters at the laser source,
is although the apparatus is capable of recording the spectra from both
fluorophores simultaneously.
The sensitivity of the scans is typically calibrated using the signal
intensity generated by a
cDNA control species added to the probe mix at a known concentration. A
specific location on the
array contains a complementary DNA sequence, allowing the intensity of the
signal at that location to
be correlated with a weight ratio of hybridizing species of 1:200,000. When
two probes from different
a o sources (e.g., representing test and control cells), each labeled with a
different fluorophore, are
hybridized to a single array for the purpose of identifying genes that are
differentially expressed, the
calibration is done by labeling samples of the calibrating cDNA with the two
fluorophores and adding
identical amounts of each to the hybridization mixture.
The output of the photomultiplier tube is digitized using a 12-bit RTI-835H
analog-to-digital
as (A/D) conversion board (Analog Devices, Inc., Norwood, MA) installed in an
IBM-compatible PC
computer. The digitized data are displayed as an image where the signal
intensity is mapped using a
linear 20-color transformation to a pseudocolor scale ranging from blue (low
signal) to red (high
signal). The data is also analyzed quantitatively. Where wo different
fluorophores are excited and
measured simultaneously, the data are first corrected for optical crosstalk
(due to overlapping emission
3 o spectra) between the fluorophores using each fluorophore's emission
spectrum.
A grid is superimposed over the fluorescence signal image such that the signal
from each spot
is centered in each element of the grid. The fluorescence signal within each
element is then
integrated to obtain a numerical value corresponding to the average intensity
of the signal. The
62
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
software used for signal analysis is the GEMTOOLS gene expression analysis
program (Incyte).
XII. Complementary Nucleic Acids
Sequences complementary to the sptm are used to detect, decrease, or inhibit
expression of
s the naturally occurring nucleotide. The use of oligonucleotides comprising
from about 15 to 30 base
pairs is typical in the art. However, smaller or larger sequence fragments can
also be used.
Appropriate oligonucleotides are designed from the sptm using OLIGO 4.06
software (National
Biosciences) or other appropriate programs and are synthesized using methods
standard in the art or
ordered from a commercial supplier. To inhibit transcription, a complementary
oligonucleotide is
to designed from the most unique 5' sequence and used to prevent transcription
factor binding to the
promoter sequence. To inhibit translation, a complementary oligonucleotide is
designed to prevent
ribosomal binding and processing of the transcript.
XIII. Expression of SPTM
15 Expression and purification of SPTM is accomplished using bacterial or
virus-based
expression systems. For expression of SPTM in bacteria, cDNA is subcloned into
an appropriate
vector containing an antibiotic resistance gene and an inducible promoter that
directs high levels of
cDNA transcription. Examples of such promoters include, but are not limited
to, the trp-lac (tac)
hybrid promoter and the TS or T7 bacteriophage promoter in conjunction with
the lac operator
2o regulatory element. .Recombinant vectors are transformed into suitable
bacterial hosts, e.g.,
BL21 (DE3). Antibiotic resistant bacteria express SPTM upon induction with
isopropyl beta-D- '
thiogalactopyranoside (IPTG). Expression of SPTM in eukaryotic cells is
achieved by infecting insect
or mammalian cell lines with recombinant Auto~raphica californica nuclear
polyhedrosis virus
(AcMNPV), commonly known as baculovirus. The nonessential.polyhedrin gene of
baculovirus is
a s replaced with cDNA encoding SPTM by either homologous recombination or
bacterial-mediated
transposition involving transfer plasmid intermediates. Viral infectivity is
maintained and the strong
polyhedrin promoter drives high levels of cDNA transcription. Recombinant
baculovii~us is used to
infect Spodoptera fru~iperda (Sf9) insect cells in most cases, or human
hepatocytes, in some cases.
Infection of the latter requires additional genetic modifications to
baculovirus. (See e.g., Engelhard,
s o supra; and Sandig, supra.)
In most expression systems, SPTM is synthesized as a fusion protein with,
e.g., glutathione S-
transferase (GST) or a peptide epitope tag, such as FLAG or 6-His, permitting
rapid, single-step,
affinity-based purification of recombinant fusion protein from crude cell
lysates. GST, a 26-kilodalton
63
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
enzyme from Schistosoma japonicum, enables the purification of fusion proteins
on immobilized
glutathione under conditions that maintain protein activity and antigenicity
(Amersham Pharmacia
Bioteeh). Following purification, the GST moiety can be proteolytically
cleaved from SPTM at
specifically engineered sites. FLAG, an 8-amino acid peptide, enables
immunoaffinity purification
s using commercially, available monoclonal and polyclonal anti-FLAG antibodies
(Eastman Kodak
Company, Rochester NY). 6-His, a stretch of six consecutive histidine
residues, enables purification
on metal-chelate resins (QIAGEN). Methods for protein expression and
purification are discussed in
Ausubel (1995, supra, Chapters 10 and 16). Purified SPTM obtained by these
methods can be used
directly in the following activity assay.
XIV. Demonstration of SPTM Activity
An assay for SPTM activity measures the expression of SPTM on the cell
surface. cDNA
encoding SPTM is subcloned into an appropriate mammalian expression vector
suitable for high levels
of eDNA expression. The resulting construct is transfected into a nonhuman
cell line such as
NIH3T3. Cell surface proteins are labeled with biotin using methods known in
the art.
Immunoprecipitations are performed using SPTM-specific antibodies, and
immunoprecipitated samples
are analyzed using SDS-PAGE and immunoblotting techniques. The ratio of
labeled
immunoprecipitant to unlabeled immunoprecipitant is proportional to the amount
of SPTM expressed
on the cell surface.
2 o Alternatively, an assay for SPTM activity measures the amount of SPTM in
secretory,
membrane-bound organelles: Transfected cells as described above are harvested
and lysed. The
lysate is fractionated using methods known to those of skill in the art, for
example, sucrose gradient
ultracentrifugation. Such methods allow the isolation of subcellular
components such as the Golgi
apparatus, ER, small membrane-bound vesicles, and other secretory organelles.
Immunoprecipitations
as from fractionated and total cell lysates are performed using SPTM-specific
antibodies, and
immunoprecipitated samples are analyzed using SDS-PAGE and immunoblotting
techniques: The
concentration of SPTM in secretory organelles relative to SPTM in total cell
lysate is proportional to
the amount of SPTM in transit through the secretory pathway.
3 o XV. Functional Assays
SPTM function is assessed by expressing sptm at physiologically elevated
levels in
mammalian cell culture systems. cDNA is subcloned into a mammalian expression
vector containing
a strong promoter that drives high levels of cDNA expression. 'Vectors of
choice include pCMV
64
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
SPORT (Life Technologies) and pCR3.1 (Invitrogen Corporation, Carlsbad CA),
both of which
contain the cytomegalovirus promoter. 5-10 ~ g of recombinant vector are
transiently transfected into
a human cell line, preferably of endothelial or hematopoietic origin, using
either liposome formulations
or electroporation. 1-2 ~ g of an additional plasmid containing sequences
encoding a marker protein
s are co-transfected.
Expression of a marker protein provides a means to distinguish transfected
cells from
nontransfected cells and is a reliable predictor of cDNA expression from the
recombinant vector.
Marker proteins of choice include, e.g., Green Fluorescent Protein (GFP;
CLONTECH), CD64, or a
CD64-GFP fusion protein. Flow cytometry (FCM), an automated laser optics-based
technique, is
to used to identify transfected cells expressing GFP or CD64-GFP and to
evaluate the apoptotic state of
the cells and other cellular properkies.
FCM detects and quantifies the uptake of fluorescent molecules that diagnose
events
preceding or coincident with cell death. These events include changes in
nuclear DNA content as .
measured by staining of DNA.with propidium iodide; changes in cell size and
granularity as measured
15 by forward light scatter and 90 degree side light scatter; down-regulation
of DNA synthesis as
measured by decrease in bromodeoxyuridine uptake; alterations in expression of
cell surface and
intracellular proteins as measured by reactivity with specific antibodies; and
alterations in plasma
membrane composition as measured by the binding of fiuorescein-conjugated
Annexin V protein to the
cell surface. Methods. in flow cytometry are discussed in OrW erod, M. G.
(1994) Flow Cytometr~,
2 o Oxford, New York NY.
The influence of SPTM on gene expression' can be assessed using highly
purified populations
of cells transfected with sequences encoding SPTM and either CD64 or CD64-GFP.
CD64 and
CD64-GFP are expressed on the surface of transfected cells and bind to
conserved regions of human
immunoglobulin G (IgG). Transfected cells are efficiently separated from
nontransfected cells using
as magnetic beads coated with either human IgG or antibody against CD64
(DYNAL, Inc., Lake
Success NY). mRNA can be purified from the cells using methods well known by
those of skill in the
art. Expression of mRNA encoding SPTM and other genes of interest can be
analyzed by northern
analysis or microarray techniques.
3 o XVI. Production of Antibodies
SPTM substantially purified using polyacrylamide gel electrophoresis (PAGE;
see, e.g.,
Harrington, M.G. (1990) Methods Enzymol. 182:488-495), or other purification
techniques, is used to
immunize rabbits and to produce antibodies using standard protocols.
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
Alternatively, the SPTM amino acid sequence is analyzed using LASERGENE
software
(DNASTAR) to determine regions of high immunogenicity, and a corresponding
peptide is synthesized
and used to raise antibodies by means known to those of skill in the art.
Methods for selection of
appropriate epitopes, such as those near the C-terminus or in hydrophilic
regions are well described in
s the art. (See, e.g., Ausubel, 1995, supra, Chapter 11.)
Typically, peptides 15 residues in length are synthesized using an ABI 431A
peptide
synthesizer (Applied Biosystems) using fmoc-chemistry and coupled to I~LH
(Sigma) by reaction with
N-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) to increase
immunogenicity. (See, e.g.;
Ausubel, supra.) Rabbits are immunized with the peptide-KLH complex in
complete Freund's
to adjuvant. Resulting antisera are tested for antipeptide activity by, for
example, binding the peptide to
plastic, blocking with 1 % BSA, reacting with rabbit antisera, washing, and
reacting with radio-
iodinated goat anti-rabbit IgG. Antisera with antipeptide activity are tested
for anti-SPTM activity
using protocols well known in the art, including ELISA, RIA, and
immunoblotting.
15 XVII. Purification of Naturally Occurring SPTM Using Specific Antibodies
Naturally occurring or recombinant SPTM is substantially purified by
immunoaffinity
chromatography using antibodies specific for SPTM. An immunoaffinity column is
constructed by
covalently coupling anti-SPTM antibody to an activated chromatographic resin,
such as
CNBr=activated SEPHAROSE (Amersham Pharmacia Biotech). After the coupling, the
resin is
2 o blocked and washed according to the manufacturer's instructions.
Media containing SPTM are passed over the immunoaffinity column, and the
column is
washed under conditions that allow the preferential absorbance of SPTM (e.g.,
high ionic strength
buffers in the presence of detergent). The column is eluted under conditions
that disrupt
antibody/SPTM binding (e.g., a buffer of pH 2 to pH 3, or a high concentration
of a chaotrope, such as
2 s urea or thiocyanate ion), and SPTM is collected.
XVIII. Identification of Molecules Which Interact with SPTM
SPTM, or biologically active fragments thereof, are labeled with lzsI Bolton-
Hunter reagent.
(See, e.g., Bolton, A.E. and W.M. Hunter (1973) Biochem. J. 133:529-539.)
Candidate molecules
3 o previously arrayed in the wells of a multi-well plate are incubated with
the labeled SPTM, washed,
and any wells with labeled SPTM complex are assayed. Data obtained using
different concentrations
of SPTM are used to calculate values for the number, affinity, and association
of SPTM with the
candidate molecules.
66
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
Alternatively, molecules interacting with SPTM are analyzed using the yeast
two-hybrid
system as described in Fields, S. and O. Song (1989) Nature 340:245-246, or
using commercially
available kits based on the two-hybrid system, such as the MATCHMAKER system
(CLONTECH).
SPTM may also be used in the PATHCALLING process (CuraGen Corp., New Haven CT)
s which employs the yeast two-hybrid system in a high-throughput manner to
determine all interactions
between the proteins encoded by two large libraries of genes (Nandabalan, K.
et al. (2000) U.S.
Patent No. 6,057,101).
All publications and patents mentioned in the above specification are herein
incorporated by
to reference. Various modifications and variations of the described method and
system of the invention
will be apparent to those skilled in the art without departing from the scope
and spirit of the invention.
Although the invention has been described in connection with specific
preferred embodiments, it
should be understood that the invention as claimed should not be unduly
limited to such specific
embodiments. Indeed, various modifications of the above-described modes for
carrying out the
is invention which are obvious to those skilled in the field of molecular
biology or related fields are
intended to be within the scope of the following claims.
67
CA 02419943 2003-02-20
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TABLE 1
SEQ ID Template ID SEQ ID ORF ID
NO; NO:
1 LG:983076.3:2000SEP08185 LG:983076.3.orf3:2000SEP08
2 LG:1382987.7:2000SEP08186 LG;1382987.7.orf2:2000SEP08
3 LG:235557.15:2000SEP08187 LG:235557.15,orf2;2000SEP08
4 LG;018494.1:2000SEP08188 LG:018494.1.orf2:2000SEP08
LG;980494.1:2000SEP08189 LG:980494.1.orf3:2000SEP08
b LG;984457.2:2000SEP08190 LG:984457.2.orf1:2000SEP08
7 LG;406758.1;2000SEP08191 LG;40b758.1.orf1;2000SEP08
8 LG;902957.17;2000SEP08192 LG;902957.17.orf3:2000SEP08
9 LG:333 T 79. T 193 LG:333179. T .ort3;2000SEP08
;2000SEP08
LG;4065b8.1:2000SEP08194 LG;4065b8.1.orf2:2000SEP08
11 LG;353203.1:2000SEP08195 LG:353203.1.orf1:2000SEP08
12 LG;061277.1;2000SEP08196 LG;ObI 277.1.orf3;2000SEP08
13 LG:170666.1:2000SEP08197 LG:170b66.1.orf2:2000SEP08
14 LG:311197.1:2000SEP08198 LG;311197.1.orf2;2000SEP08
LG:220655.4:2000SEP08199 LG:220b55.4.orf2:2000SEP08
T 6 LG;1001893.1;2000SEP08200 LG; i 00 T 893, i
.orf2:2000SEP08
17 LG:004335.1:2000SEP08201 LG:004335.1.orf1:2000SEP08
18 LG:213092.6:2000SEP08202 LG:213092.b.orf2:2000SEP08
19 LG:407570.5;2000SEP08203 LG:407570.5.orf3;2000SEP08
LG;337835.8:2000SEP08204 LG;337835.8.orf1;2000SEP08
21 LG;1099283.1;2000SEP08205 LG:1099283.1.orf2;2000SEP08
22 LG;401274.2;2000SEP08206 LG;401274.2.orf2;2000SEP08
23 LG;222880.1:2000SEP08207 LG;222880.1.orf3;2000SEP08
24 LG:406389.1:2000SEP08208 LG;406389.1.orf1:2000SEP08
LG:055461.7 ;2000SEP08209 LG:055467.1.orf2:2000SEP08
26 LG:979059.5:2000SEP08210 LG:979059.5.orf1:2000SEP08
27 LG;399238.1;2000SEP08211 LG;399238.1.orf1:2000SEP08
28 LG:1382945.7;2000SEP08212 LG:1382945.7.orf1:2000SEP08
29 LG:1383b10.3;2000SEP08213 LG:1383610.3.orf2:2000SEP08
LG:1384030.1;2000SEP08214 LG;1384030.1.orf3;2000SEP08
31 LG:390475.1:2000SEP08215 LG;390475.1.orf1:2000SEP08
32 LG;229105.3:2000SEP08216 LG:229105.3.orf2;2000SEP08
33 LG:232578.3;2000SEP08217 LG;232578.3.orf1:2000SEP08
34 LG;1166387.9:2000SEP08218 LG:1166387.9.orf1:2000SEP08
LG:351357.1;2000SEP08219 LG:351357.1.orf1:2000SEP08
36 LG:465592.1:2000SEP08220 LG;465592.1.orf1:2000SEP08
37 LG:OOb848.5:2000SEP08221 LG:OOb848.5.orf1;2000SEP08
38 LG;198450.2;2000SEP08222 LG:198450.2.orf2:2000SEP08
39 LG:1008175.1:2000SEP08223 LG:1008175.1.orf1;2000SEP08
LG:437981.11:2000SEP08224 LG;437981.11.orf2;2000SEP08
41 LG;1025549.1;2000SEP08225 LG:1025549.1.orf3:2000SEP08
42 LG;327226.16:2000SEP08226 LG:327226.1 b.orfl
;2000SEP08
43 LG:1387394.5;2000SEP08227 LG:1387394.5.orf3;2000SEP08
44 LG:445188.3:2000SEP08228 LG;445i88.3.ort3;2000SEP08
LG;898864.11:2000SEP08229 LG;898864.11.orf3:2000SEP08
46 LG:0T8739.2:2000SEP08230 LG:018739.2.orf1;2000SEP08
'
47 LG:302915.6:2000SEP08231 LG:302915.b.orf3:2000SEP08
48 LG:404418.3;2000SEP08232 LG;404418.3.orf1;2000SEP08
49 LG:374853.2:2000SEP08233 LG;374853.2.orf1:2000SEP08
LG:228930.1:2000SEP08234 LG:228930.1.ort2:2000SEP08
6S
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 1
SEQ ID Template ID SEQ ID ORF ID
NO; NO:
51 LG:273593.6;2000SEP08235 LG:273593.b.orf1;2000SEP08
52 LG:008215.1;2000SEP08236 LG;008215.1.orf3;2000SEP08
53 LG:337160.1:2000SEP08237 LG;337160.1.orf1:2000SEP08
54 LG:3950b3.1;2000SEP08238 LG:3950b3, l .orf3:2000SEP08
55 LG;979069.4:2000SEP08239 LG:979069.4.orf1:2000SEP08
56 LG:346663.5:2000SEP08240 LG;346663.5.orf3:2000SEP08
57 LG:347b15.1:2000SEP08241 LG:347615.1.orf3:2000SEP08
58 LG:1397067.1;2000SEP08242 LG:1397067, l .orf1;2000SEP08
59 LG:120b75.1:2000SEP08243 LG;120675.1.orf1;2000SEP08
b0 LG;420050.18;2000SEP08244 LG;420050.18.orf3;2000SEP08
61 LG:220495.3;2000SEP08245 LG;220495.3.orf1:2000SEP08
b2 LG;274551.1:2000SEP08246 LG:274551.l .orf1;2000SEP08
63 LG:429658.27:2000SEP08247 LG;429658.27.orf2:2000SEP08
64 LG;246194.18:2000SEP08248 LG;246194.18.orf1;2000SEP08
65 LG:000874.1:2000SEP08249 LG:000874, l .orf1;2000SEP08
bb LG:2399b7.7:2000SEP08250 LG:239967.7.orf1:2000SEP08
67 LG:238388.1:2000SEP08~ 251 LG:238388.1.orf3;2000SEP08
b8 LG:233674.4:2000SEP08252 LG:233674.4.orf1:2000SEP08
69 LG;411327.2:2000SEP08253 LG:411327.2.orf3;2000SEP08
70 LG:1327310.1:2000SEP08254 LG;1327310, l .orf1:2000SEP08
71 LG;242019.13:2000SEP08255 LG:242019.13.orf2;2000SEP08
72 LG:012432.12;2000SEP08256 LG:012432.12.orf1;2000SEP08
73 LG;257088.9:2000SEP08257 LG:257088.9.orf3;2000SEP08
74 LG;997505.5:2000SEP08258 LG:997505.5.orf2;2000SEP08
75 LG:48143b.2:2000SEP08259 LG:481436.2.orf3:2000SEP08
76 LG:247776.14:2000SEP08260 LG;24777b.14.orf1:2000SEP08
77 LG;008606.14;2000SEP08261 LG:008606.14.orf2:2000SEP08
78 LG:985092.3:2000SEP082b2 LG:985092.3.orf2:2000SEP08
79 LG;236649.7;2000SEP08263 LG:236649.7.orf2:2000SEP08
80 LG:245014.2:2000SEP08264 LG;245014.2.orf1:2000SEP08
81 LG:170754.4:2000SEPOB265 LG:170754.4.orf2;2000SEP08
82 LG;988028.1;2000SEP08266 LG;988028.1.orfl
:2000SEP08
83 LG:427997.6:2000SEP082b7 LG;427997,b.orf3;2000SEP08
84 LG;464206.1:2000SEP08268 LG;464206.1.orf2;2000SEP08
85 LG:1400108.1:2000SEP08269 LG;1400108.1.orf3:2000SEP08
86 LG:254531.1:2000SEP08270 LG:254531.1.orf1:2000SEP08
87 LG:I 101317.1;2000SEP08271 LG:1101317.l .orf1;2000SEP08
88 LG;1074728.6;2000SEP08272 LG;1074728.6.orf1;2000SEP08
89 LG:1081684.1:2000SEP08273 LG;1081684. l .orf3:2000SEP08
90 LG:1076520.1:2000SEP08274 LG;1076520.1.orf1;2000SEP08
91 LG:1079477.1:2000SEP08275 LG:1079477.1.orf2:2000SEP08
92 LG;1076269.1:2000SEP08276 LG;1076269.1.orf2;2000SEP08
93 LG:1087195.1;2000SEP08277 LG;1087195.1.orf3:2000SEP08
94 LG:002588.7:2000SEP08278 LG;002588.7.orf2;2000SEP08
95 LG:1079470.6:2000SEP08279 LG:1079470.6.orf1;2000SEP08
96 LG;345705.3;2000SEP08280 LG:345705.3.orf1:2000SEP08
97 LG:1083654.1;2000SEP08281 LG:1083654.1.orfl
;2000SEP08
98 LG:198782.3;2000SEP08282 LG;198782.3.orfl
;2000SEP08
99 LG:981076.2:2000SEP08283 LG:981076.2.orf1:2000SEP08
100 LG:212023.3:2000SEP08284 LG;212023.3.orf3:2000SEP08
69
CA 02419943 2003-02-20
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TABLE 1
SEQ ID Template ID SEQ ID ORF ID
NO: NO:
101 LG:977929.3:2000SEP08285 LG:977929.3.orf3;2000SEP08
102 LG:201936.b:2000SEP08286 LG:201936.b.ort2:2000SEP08
103 LG:205b42.1;2000SEP08287 LG:205642.1.orf3;2000SEP08
104 LG:339653.b:2000SEP08288 LG:339653.b.orf3:2000SEP08
105 LG:978587.4:2000SEP08289 LG:978587.4.orf1:2000SEP08
106 LG:216848.17:2000SEP08290 LG:216848.17.ort1:2000SEP08
107 LG;219502.1:2000SEP08291 LG:219502.1.orf2:2000SEP08
108 LI:334211.1;2000SEP08292 LI:334211.1.orf2:2000SEP08
109 LI:231024.2:2000SEP08293 LI:231024.2.orf3:2000SEP08
110 LI;228425.5;2000SEP08294 LI:228.425.5.orf2:2000SEP08
1 i 1 LI:034493.1;2000SEP08295 LI:034493.1.orf3:2000SEP08
112 LI:33b218.1;2000SEP08296 LI;33b218.1.orf2;2000SEP08
113 LI:235891.3:2000SEP08297 LI:235891.3.orf1;2000SEP08
114 LI:344094.1;2000SEP08298 LI:344094.1.orf2:2000SEP08
115 LI:399945.2:2000SEP08299 LI;399945.2.orf3;2000SEP08
116 LI;051849.1:2000SEP08300 LI:051849.1.orf1:2000SEP08
117 LI:238379.3:2000SEP08301 LI;238379.3.orf2:2000SEP08
118 LI;352190.8:2000SEP08302 LI:352190.8.orfi3;2000SEP08
119 LI;432120.1;2000SEP08303 LI;432120.1.orf2;2000SEP08
120 LI;0554b1.1:2000SEP08304 LI:0554b1.1.orf2:2000SEP08
121 LI:197433.5:2000SEP08305 LI;197433.5.orf1:2000SEP08
122 LI:170b04.1;2000SEP08306 LI:170b04.1.orf2;2000SEP08
123 LI:205057.3:2000SEP08307 LI:205057.3.orf2;2000SEP08
124 LI:233795.1:2000SEP08308 LI:233795, l .orf1;2000SEP08
125 LI;311197.1;2000SEP08309 LI;3i 1197.1.orf2:2000SEP08
126 LI:441364.1;2000SEP08310 LI:4413b4.1.orf3;2000SEP08
127 LI:2103b7.6:2000SEP08311 LI;210367.b.orf3;2000SEP08
128 LI:238194.5;2000SEP08312 LI:238194.5.ort3:2000SEP08
129 LI;039258.5:2000SEP08313 LI:039258.5.orf1;2000SEP08
130 LI:1071842.1:2000SEP08314 LI:1071842.1.orf2;2000SEP08
131 LI;481356.3;2000SEP08315 LI;481356.3.orf2:2000SEP08
132 LI;103474.1:2000SEP08316 LI:103474.1.orf3;2000SEP08
133 LI;1073020.10:2000SEP08317 LI:1073020.10.orf2:2000SEP08
134 LI;000874.1:2000SEP08318 LI:000874.1.orf1;2000SEP08
135 LI:037298.2;2000SEP08319 LI:037298.2.orf1;2000SEP08
136 LI;422901.1;2000SEP08320 LI:422901.l .orf1:2000SEP08
137 LI;345815.1;2000SEP08321 LI:345815.1.orf2:2000SEP08
138 LI:1072014.2:2000SEP08322 LI:1072014.2.orf3:2000SEP08
139 LI:333138,3;2000SEP08323 LI:333138.3.orf3;2000SEP08
140 LI:414253.1:2000SEP08324 LI:414253.1.orf3;2000SEP08
141 LI;406389.1;2000SEP08325 LI:406389.1.orf1;2000SEP08
142 LI:108b171.1:2000SEP08326 LI:108b171.l.orf1;2000SEP08
143 LI:198782.4:2000SEP08327 LI:198782.4.orf3:2000SEP08
144 LI:2030279.1:2000SEP08328 LI:2030279.1.orf2;2000SEP08
145 LI;1 Ol 8424.3:2000SEP08329 LI:1 Ol 8424.3.orf2;2000SEP08
146 LI:1309b9.1;2000SEP08330 LI:1309b9.1.orf3:2000SEP08
147 LI:28b246.2;2000SEP08331 LI:28b246.2.orf1;2000SEP08
148 LI:001527.1;2000SEP08332 LI:001527.1.orf2:2000SEP08
149 LI;395063.1:2000SEP08333 LI:395063.1.orf1:2000SEP08
150 LI:1064460,1:2000SEP08334 LI:10644b0.1.orf1:2000SEP08
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE T
SEQ ID Template ID SEQ ID ORF ID
NO: NO;
151 LI:344b90.2:2000SEP08335 LI:344b90.2,orf1:2000SEP08
152 LI:061585.4:2000SEP08336 LI:Ob1585.4.orf3;2000SEP08
153 LI:378428.1:2000SEP08337 LI:378428.1.orf1:2000SEP08
154 LI:474108.2;2000SEP08338 LI:474108,2,orf3;2000SEP08
155 LI:23071 T ,2:2000SEP08339 LI:23071 T ,2.orf1:2000SEP08
156 LI;008942.1:2000SEP08340 LI:008942.1,orf1;2000SEP08
157 LI:732479.1:2000SEP08341 LI:732479.1.orf3:2000SEP08
158 LI:1190250,1:2000SEP08342 LI:1190250,1.orf3;2000SEP08
159 LI;1013717.1:2000SEP08343 LI:1013717.l .orf1:2000SEP08
160 LI:2049T25,2:2000SEP08344 LI;2049125,2.orf1:2000SEP08
161 LI:1092360.1:2000SEP08345 LI:1092360.1.orf3:2000SEP08
162 LI:791524,1;2000SEP08346 LI:791524.1,orf2;2000SEP08
163 LI;1084555.3:2000SEP08347 LI:1084555,3,orf1;2000SEP08
164 LI:815418,2:2000SEP08348 LI:815418.2,orf1:2000SEP08
165 LI:416766.1:2000SEP08349 LI:41 6766. l .orf1:2000SEP08
166 Ll: l T 71008.2:2000SEP08350 LI:1171008.2.orf1:2000SEP08
167 LI:1169888,3:2000SEP08351 LI:1169888.3,orf2:2000SEP08
1 b8 LI:412592.1:2000SEP08352 LI;412592.1,orf3:2000SEP08
169 LI;349808,1:2000SEP08353 LI:349808.1.orf3:2000SEP08
170 LI:349164.2:2000SEP08354 LI:349164,2.ort3:2000SEP08
171 LI;205413,1:2000SEP08' 355 LI;205413.1,orf2:2000SEP08
172 LI:2051508,2:2000SEP08356 LI:2051508.2.orf1:2000SEP08
173 LI:34b242,2;2000SEP08357 LI;346242,2,orf2;2000SEP08
174 LI:2052717,1;2000SEP08358 LI;2052717.1,orf2:2000SEP08
175 LI:40bb68,2:2000SEP08359 LI;4066b8.2,ort2:2000SEP08
175 LI:406668.2:2000SEP08360 LI:40b668,2.orf3:2000SEP08
176 LI;1178352,1:2000SEP08361 LI:1178352. l .orf1;2000SEP08
177 LI:8140T4,7:2000SEP08362 LI:814014.7.orf1;2000SEP08
i 78 Li;1170624,1:2000SEP08363 LI: i 170b24,1.orf2:2000SEP08
179 LI:1183171,1;2000SEP08364 LI;1183171. l ,orf3:2000SEP08
180 LI:1093491,1:2000SEP08365 LI;1093491.1.orfl
:2000SEP08
181 LI:046515.5:2000SEP08366 LI;046515.5,orf2;2000SEP08
182 LI;400171.2:2000SEP08367 LI;400171,2.orf3;2000SEP08
183 LI:3309T9,b:2000SEP08368 LI:3309i9,6,orf3;2000SEP08
184 LI;219502,1:2000SEP08369 LI;219502.1,orf3:2000SEP08
71
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TABLE 2
SEQ ID Template ID StartStop Frame Domain TypeTopology
NO:
1 LG:983076.3:2000SEP08775 861 forwardTM N out
1
1 LG;983076.3:2000SEP08479 565 forwardTM N in
2
1 LG;983076.3:2000SEP08707 793 forwardTM N in
2
1 LG:983076.3:2000SEP08114 200 forwardTM N out
3
1 LG;98307b.3:2000SEP08261 317 forwardTM N out
3
1 LG:983076.3:2000SEP08501 587 forwardTM N out
3
1 LG;983076.3:2000SEP08738 794 forwardTM N out
3
2 LG;1382987.7:2000SEP0828 102 forwardTM N out
1
3 LG:235557.15:2000SEP0855 141 forwardTM N in
1
3 LG;235557.15;2000SEP0856 118 forwardTM N in
2
3 LG:235557.15:2000SEP08149 211 forwardTM N in
2
3 LG:235557.15:2000SEP0833 95 forwardTM N in
3
3 LG:235557.15:2000SEP08135 197 forwardTM N in
3
4 LG:018494.1;2000SEP08175 243 forwardTM N in
1
4 LG:018494.1:2000SEP08298 360 forwardTM N in
1
4 LG;018494.1:2000SEP08370 432 forwardTM N in
1
4 LG:018494.1:2000SEP08460 546 forwardTM N in
1
4 LG;018494.1;2000SEP0829 115 forwardTM N out
2
4 LG:018494.1;2000SEP08188 274 forwardTM N out
2
4 LG:018494.1:2000SEP08347 409 forwardTM N out
2
4 LG:018494.1:2000SEP08.446508 forwardTM N out
2
4 LG;018494.1:2000SEP0824 101 forwardTM N in
3
4 LG:018494.1;2000SEP08345 431 forwardTM N in
3
4 LG;018494.1:2000SEP08453 509 forwardTM N in
3
4 LG;018494.1;2000SEP08567 653 forwardTM N in
3
LG:980494.1;2000SEP08304 357 forwardTM N in
1
5 LG:980494.1:2000SEP0812671335 forwardTM N in
1
5 LG:980494.1:2000SEP0813391410 forwardTM N in
1
5 LG;980494.1;2000SEP0814531524 forwardTM N in
1
5 LG:980494.1:2000SEP0815641611 forwardTM N in
' 1
5 LG:980494.1;2000SEP08563 628 forwardTM N out
2
5 LG:980494.1:2000SEP0813641450 forwardTM N out
2
5 LG:980494.1:2000SEP0815 101 forwardTM N in
3
5 LG:980494.1:2000SEP08855 941 forwardTM N in
3
5 LG:980494.1:2000SEP0812511337 forwardTM N in
3
5 LG:980494.1;2000SEP0813741424 forwardTM N in
3
5 LG:980494.1:2000SEP0814701523 forwardTM N in
3
6 LG;984457.2:2000SEP08418 504 forwardTM N out
1
b LG:984457.2:2000SEP08658 738 forwardTM N out
1
b LG:984457.2:2000SEP08889 975 forwardTM N out
1
6 LG:984457.2:2000SEP0811261188 forwardTM N out
1
6 LG;984457.2:2000SEP08765 836 forwardTM N in
3
7 LG:406758.1;2000SEP0810721146 forwardTM N out
1
7 LG:406758.1:2000SEP0812281314 forwardTM N out
1
7 LG:40b758.1:2000SEP08767 847 forwardTM N in
2
7 LG:40b758.1:2000SEP0866 119 forwardTM N out
3
7 LG:406758.1;2000SEP08753 824 forwardTM N out
3
7 LG:406758.1;2000SEP08849 911 forwardTM N out
_ -~ . 3
__
72
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TABLE 2
SEQ ID Template ID StartStop Frame Domain TypeTopology
NO;
7 LG:40b758.1:2000SEP08T T forwardTM N out
059 T 3
2
T
8 LG;902957.17:2000SEP08145 216 forwardTM N out
1
8 LG:902957.17;2000SEP08lbl 232 forwardTM N out
2
8 LG;902957.17;2000SEP0818 77 forwardTM N out
3
8 LG:902957.17;2000SEP08132 218 forwardTM N out
3
9 LG:333179.1:2000SEP08109 171 forwardTM N out
1
9 LG:333179.1:2000SEP08211 273 forwardTM N out
1
9 LG;333179.1:2000SEP08334 381 forwardTM N out
1
9 LG;333179.1;2000SEP08600 647 forwardTM N out
3
LG:40b568.1:2000SEP0819342017 forwardTM N out
2
10 LG;406568.1:2000SEP0820932173 forward~ TM N out
2
10 LG:406568.1;2000SEP08T 59 forwardTM N out
2 3
10 LG;4065b8.1:2000SEP08498 557 forwardTM N out
3
10 LG;406568.1;2000SEP081740.T826forwardTM N out
3
10 LG:4065b8.1;2000SEP0818331907 forwardTM N out
3
10 LG:406568.1:2000SEP0819262012 forwardTM N out
3
11 LG:353203.1:2000SEP0895 151 forwardTM N in
2
12 LG;Ob1277.1:2000SEP08217 303 forwardTM N out
1
13 LG:170666.1;2000SEP0884 134 forwardTM N out
3
14 LG:311197.1:2000SEP08241 315 forwardTM N in
1
14 LG;311197.1:2000SEP08527 613 forwardTM N out
2
LG;220655.4:2000SEP08200 2b8 forwardTM N in
2
15 LG:220b55.4:2000SEP08341 403 forwardTM N in
2
15 LG:220655.4;2000SEP08198 284 forwardTM N out
3
15 LG;220b55.4;2000SEP08675 722 forwardTM N out
3
16 LG:1001893.1:2000SEP08370 447 forwardTM N out
1
16 LG:1001893.1:2000SEP08463 534 forwardTM N out
1
16 LG:1001893.1:2000SEP08405 491 forwardTM N in
3
16 LG:1001893.1;2000SEP08666 752 forwardTM N in
3
17 LG:004335.1:2000SEP08607 660 forwardTM N in
1
17 LG:004335.1;2000SEP08877 9b3 forwardTM N in
1
T 7 LG;004335.1:2000SEP0814021485 forwardTM N in
1
17 LG:004335.1:2000SEP0814921578 forwardTM N in
1
T 7 LG;004335.1;2000SEP0817831839 forwardTM N in
1
17 LG:004335.1:2000SEP0821672253 forwardTM N in
1
17 LG;004335.1;2000SEP0812981384 forwardTM N in
2
17 LG:004335.1:2000SEP0814151486 forwardTM N in
2
17 LG:004335.1;2000SEP0823002386 forwardTM N in
2
17 LG:004335.1:2000SEP0811851241 forwardTM N out
3
17 LG;004335.1;2000SEP0814161502 forwardTM N out
3
17 LG:004335.1;2000SEP0816201685 forwardTM N out
3
17 LG;004335.1:2000SEP0818421925 forwardTM N out
3
17 LG;004335.1;2000SEP0823162393 forwardTM N out
3
18 LG:213092.b;2000SEP08217 303 forwardTM N out
1
19 LG:407570.5:2000SEP08433 519 forwardTM N in
1
19 LG;407570.5:2000SEP0811 85 forwardTM N out
2
19 LG;407570.5;2000SEP08440 493 forwardTM N out
2
19 LG:407570.5:2000SEP08569 625 forwardTM N out
- _ _ 2
.-. _
73
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TABLE 2
SEQ ID Template ID StartStopFrame Domain Topology
NO: Type
19 LG;407570.5:2000SEP08414 500 forwardTM N out
3
20 LG:337835.8:2000SEP0870 141 forwardTM N out
1
21 LG:1099283.1:2000SEP0856 133 forwardTM N in
2
21 LG:1099283.1:2000SEP08173 259 forwardTM N in
2
22 LG;401274.2:2000SEP08292 348 forwardTM N in
1
23 LG:222880.1:2000SEP08211 297 forwardTM
1
23 LG:222880.1:2000SEP081714 1788forwardTM
1
23 LG;222880.1:2000SEP081813 1899forwardTM
1
23 LG:222880.1:2000SEP081930 2013forwardTM
1
23 LG;222880.1:2000SEP082068 2133forwardTM
1
23 LG:222880.1;2000SEP081b67 1753forwardTM N out
2
23 LG;222880.1;2000SEP081805 18b7forwardTM N out
2
23 LG:222880.1;2000SEP081910 1972forwardTM N out
2
23 LG:222880.1:2000SEP082273 2320forwardTM N out
2
23 LG:222880.1;2000SEP08423 485 forwardTM N in
3
23 LG:222880.1:2000SEP08504 566 forwardTM N in
3
23 LG:222880.1;2000SEP08609 689 forwardTM N in
3
23 LG:222880.1;2000SEP08696 782 forwardTM N in
3
23 LG:222880.1:2000SEP08873 959 forwardTM N in
3
23 LG:222880.1:2000SEP081014 1076forwardTM N in
3
23 LG:222880.1:2000SEP081104 1166forwardTM N in
3
23 LG:222880.1:2000SEP081542 1628forwardTM N in
3
23 LG:222880.1;2000SEP081689 1772forwardTM N in
3
23 LG:222880.1:2000SEP081938 2024forwardTM N in
3
23 LG;222880,1;2000SEP082313 2393forwardTM N in
3
24 LG:406389.1:2000SEP0897 171 forwardTM N in
1
24 LG;40b389.1;2000SEP08481 5b7 forwardTM N in
1
24 LG:406389.1:2000SEP08574 660 forwardTM N in
1
24 LG:40b389.1;2000SEP081517 1585forwardTM N out
2
24 LG:406389.1:2000SEP08690 758 forwardTM N in
3
24 LG:40b389.1:2000SEP081485 1565forwardTM N in
3
25 LG:055461.1:2000SEP08322 408 forwardTM N in
1
25 LG:055461.1:2000SEP08131 193 forwardTM N out
2
25 LG:055461.1:2000SEP08227 289 forwardTM N out
2
25 LG:055461.1:2000SEP08650 709 forwardTM N out
2
25 LG:055461.1:2000SEP081400 1462forwardTM N out
2
25 LG;055461.1:2000SEP08117 200 forwardTM N out
3
25 LG:055461.1:2000SEP08324 383 forwardTM N out
3
25 LG:055461.1;2000SEP081326 1397forwardTM N out
3
26 LG:979059.5:2000SEP08448 522 forwardTM N out
1
27 LG:399238.1:2000SEP08280 342 forwardTM N in
1
27 LG:399238.1:2000SEP08370 432 forwardTM N in
1
27 LG;399238.1;2000SEP08469 531 forwardTM N in
1
27 LG:399238.1:2000SEP08550 612 forwardTM N in
1
27 LG;399238,1:2000SEP08658 744 forwardTM N in
1
27 LG:399238.1;2000SEP081559 1645forwardTM N out
2
27 LG:399238.1:2000SEP08177 230 forwardTM
3
27 LG;399238.1;2000SEP081551 1637forwardTM
3
74
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WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain TypeTopology
NO: Stop
28 LG:1382945.7:2000SEP0873 159 forwardTM N in
)
28 LG;1382945.7;2000SEP08229 303 forwardTM N in
1
28 LG:1382945.7:2000SEP0877 139 forwardTM N out
2
28 LG;1382945.7;2000SEP08152 214 forwardTM N out
2
28 LG:1382945.7:2000SEP08251 319 forwardTM N out
2
28 LG;1382945.7;2000SEP0866 152 forwardTM N out
3
28 LG:1382945.7:2000SEP08237 296 forwardTM N out
3
29 LG:1383b10.3:2000SEP08565 624 forwardTM N out
1
29 LG:1383b10.3:2000SEP08916 993 forwardTM N out
1
29 LG;1383610.3:2000SEP081126 1197forwardTM N out
1
29 LG:1383610.3:2000SEP081471 1557forwardTM N out
1
29 LG;1383610.3:2000SEP081861 1923forwardTM N out
1
29 LG;1383610.3:2000SEP081969 2031forwardTM N out
1
29 LG:1383610.3:2000SEP082053 2121forwardTM N out
1
29 LG:1383b10.3;2000SEP082185 2247forwardTM N out
1
29 LG;1383610.3;2000SEP082275 2337forwardTM N out
1
29 LG:1383610.3:2000SEP082365 2427forwardTM N out
1
29 LG:1383610.3:2000SEP081460 1546forwardTM
2
29 LG:1383610.3:2000SEP081658 1744forwardTM
2
29 LG;1383610.3:2000SEP081832 1918forwardTM
2
29 LG:1383610.3;2000SEP081934 1996forwardTM
2
29 LG:1383610.3:2000SEP082021 2083forwardTM
2
29 LG:1383610.3;2000SEP082153 2239forwardTM
2
29 LG:1383610.3:2000SEP082309 2371forwardTM
2
29 LG:1383610.3;2000SEP082399 2461forwardTM
2
29 LG:1383610.3:2000SEP08192 278 forwardTM N out
3
29 LG:13836 i 0.3;2000SEP08i i forwardTM N out
491 577 3
29 LG;1383b10.3:2000SEP081824 1889forwardTM N out
3
29 LG:1383610.3;2000SEP081926 2012forwardTM N out
3
29 LG:1383610.3:2000SEP082067 2153forwardTM N out
3
29 LG;1383610.3;2000SEP082187 2273forwardTM N out
3
29 LG;1383610.3:2000SEP082319 2405forwardTM N out
3
30 LG:1384030.1:2000SEP08370 420 forwardTM N out
1
30 LG:1384030. i 820 870 forwardTM N out
;2000SEP08 1
30 ~LG:1384030.1;2000SEP081069 1155forwardTM N out
1
30 LG:1384030.1:2000SEP081252 1308forwardTM N out
1
30 LG:1384030.1:2000SEP081396 1470forwardTM N out
1
30 LG:1384030.1;2000SEP081561 1632forwardTM N out
1
30 LG:1384030.1:2000SEP081810 1896forwardTM N out
1
30 LG:1384030.1;2000SEP082203 2286forwardTM N out
1
30 LG:1384030. i 83 1 forwardTM N in
:2000SEP08 b0 2
30 LG;1384030.1:2000SEP081046 1120forwardTM N in
2
30 LG:1384030.1:2000SEP081145 1219forwardTM N in
2
30 LG;1384030.1;2000SEP081376 1462forwardTM N in
2
30 LG:1384030.1:2000SEP081796 1882forwardTM N in
2
30 LG;1384030.1;2000SEP082003 2089forwardTM N in
2
30 LG:1384030.1:2000SEP08924 1007forwardTM
3
30 LG:1384030.1:2000SEP081083 1169forwardTM
_ . 3
__-_
75
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WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain TypeTopology
NO: Stop
30 LG:1384030.1:2000SEP081185 1271forwardTM
3
30 LG;1384030.1;2000SEP081419 1505forwardTM
3
30 LG:1384030.1:2000SEP081572 1658forwardTM
3
30 LG:1384030.1;2000SEP081878 19b4forwardTM
3
30 LG;1384030.1:2000SEP082079 2144forwardTM
3
31 LG:390475.1;2000SEP08559 621 forwardTM N in
1
31 LG:390475.1;2000SEP081648 1704forwardTM N in
1
31 LG;390475.1;2000SEP08542 592 forwardTM N out
2
31 LG:390475.1;2000SEP08935 1021forwardTM N out
2
31 LG:390475.1;2000SEP081217 1291forwardTM N out
2
31 LG;390475.1:2000SEP08600 686 forwardTM
3
31 LG;390475.1:2000SEP081053 1103forwardTM
3
31 LG:390475.1;2000SEP081182 1268forwardTM
3
32 LG:229i05.3:2000SEP08592 642 forwardTM N out
1
32 LG;229105.3;2000SEP08578 664 forwardTM N out
2
33 LG:232578.3:2000SEP081 228 forwardTM N in
b3 1
33 LG:232578.3;2000SEP08226 276 forwardTM N in
1
33 LG:232578.3;2000SEP08295 375 forwardTM N in
1
33 LG;232578.3:2000SEP081042 1119forwardTM N in
1
33 LG;232578.3:2000SEP08155 241 forward' TM N in
2
33 LG:232578.3;2000SEP08308 376 forwardTM N in
2
33 LG:232578.3:2000SEP08599 685 forwardTM N in
2
33 LG:232578.3:2000SEP08704 781 forwardTM N in
2
33 LG;232578.3:2000SEP08998 1084forwardTM N in
2
33 LG:232578.3:2000SEP081154 1240forwardTM N in
2
33 LG:232578.3:2000SEP081 239 forwardTM N in
b5 3
33 LG:232578.3;2000SEP08327 398 forwardTM N in
3
33 LG:232578.3;2000SEP08597 647 forwardTM N in
3
33 LG;232578.3;2000SEP08720 770 forwardTM N in
3
33 LG;232578.3;2000SEP081008 1094forwardTM N in
3
33 LG:232578.3;2000SEP081128 1214forwardTM N in
3
34 LG:1166387.9;2000SEP08565 615 forwardTM N out
1
34 LG;1166387.9;2000SEP08129 188 forwardTM
3
35 LG:351357.1:2000SEP08310 396 forwardTM
1
35 LG;351357.1;2000SEP08467 529 forwardTM N in
2
35 LG;351357.1:2000SEP08557 619 forwardTM N in
2
35 LG:351357.1:2000SEP08674 736 forwardTM N in
2
35 LG:351357.1:2000SEP08761 823 forwardTM N in
2
35 LG:351357.1:2000SEP08941 1021forwardTM N in
2
35 LG;351357.1:2000SEP08186 257 forwardTM N in
3
35 LG;351357.1:2000SEP081119 1199forwardTM N in
3
36 LG:4b5592.1;2000SEP08163 225 forwardTM N in
1
36 LG:465592.1;2000SEP08247 309 forwardTM N in
1
36 LG:465592.1:2000SEP08409 480 forwardTM N in
1
36 LG;4b5592.1;2000SEP0844 94 forwardTM N in
2
36 LG;465592.1;2000SEP08221 301 forwardTM N in
2
36 LG:465592.1;2000SEP0836 104 forwardTM N in
3
37 LG:OOb848.5:2000SEP08628 b90 forwardTM N out
1
76
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID StartStopFrame Domain Topology
NO: Type
38 LG;198450.2;2000SEP08245 331 forward TM N out
2
38 LG;198450.2:2000SEP08416 469 forward TM N out
2
38 LG:198450.2:2000SEP08803 889 forward TM N out
2
38 LG;198450.2;2000SEP08750 818 forward TM N in
3
39 LG:1008175.1:2000SEP0825 87 forward TM N out
1
39 LG:1008175.1:2000SEP08115 177 forward TM N out
1
39 LG;1008175.1;2000SEP08220 297 forward TM N out
1
39 LG:1008175.1:2000SEP08352 438 forward TM N out
1
39 LG;1008175.1;2000SEP08478 564 forward TM N out
1
39 LG:1008175.1:2000SEP08649 705 forward TM N out
1
39 LG;1008175.1;2000SEP08844 900 forward TM N out
1
39 LG:1008175.1:2000SEP08407 475 forward TM N in
2
39 LG:1008175.1;2000SEP08114 200 forward TM N out
3
39 LG:1008175.1:2000SEP08309 392 forward TM N out
3
40 LG;437981.1 i 17 79 forward TM N out
;2000SEP08 2
40 LG:437981.11;2000SEP0895 157 forward TM N out
2
40 LG;437981.11:2000SEP08381 467 forward TM
3 1
41 LG;1025549.1;2000SEP08161 241 forward TM N out
2
41 LG:1025549.1:2000SEP08468 530 forward TM N out
3
41 LG;1025549.1;2000SEP08540 602 forward TM N out
3
42 LG;327226.16:2000SEP08178 246 forward TM N in
1
42 LG;327226.16:2000SEP08256 312 forward TM N in
1
42 LG:327226.16:2000SEP08325 387 forward TM N in
1
42 LG:327226.16;2000SEP08403 465 forward TM N in
1
42 LG;327226.16:2000SEP08224 301 forward TM N in
2
42 LG:327226.16:2000SEP08353 439 forward TM N in
2
42 LG:327226.16:2000SEP08252 311 forward TM N in
3
43 LG:1387394.5:2000SEP08475 5b1 forward TM N in
1
43 LG:1387394.5:2000SEP08619 705 forward TM N in
1
43 LG;1387394.5:2000SEP08591 653 forward TM N in
3
43 LG:1387394.5;2000SEP086b9 731 forward TM N in
3
44 LG:445188.3;2000SEP08124 177 forward TM N out
1
44 LG;445188.3;2000SEP08196 258 forward TM N out
1
44 LG:445188.3:2000SEP08271 333 forward TM N out
1
44 LG;445188.3:2000SEP08346 408 forward TM N out
1
44 LG:445188.3;2000SEP08i 187 forward TM N in
16 2
44 LG:445188.3:2000SEP08467 526 forward TM N in
2
44 LG:445188.3;2000SEP08102 188 forward TM N in
3
44 LG:445188.3:2000SEP08195 281 forward TM N in
3
44 LG:445188.3;2000SEP08285 350 forward TM N in
3
44 LG;445188.3:2000SEP08390 476 forward TM N in
3
45 LG;8988b4.11:2000SEP08i06 192 forward TM N out
1
45 LG;8988b4.11:2000SEP0850 133 forward TM N out
2
46 LG:018739.2;2000SEP08124 189 forward TM N in
1
46 LG:018739.2:2000SEP08454 516 forward TM N in
1
46 LG:018739.2:2000SEP08553 615 forward TM N in
T
46 LG:018739.2;2000SEP08128 208 forward TM
2
46 LG:018739.2:2000SEP08536 598 forward TM
2
77
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID StartStop Frame Domain Topology
NO; Type
46 LG:018739.2:2000SEP08623 685 forwardTM
2
46 LG:018739.2:2000SEP08234 305 forwardTM N out
3
46 LG;018739.2:2000SEP08402 488 forwardTM N out
3
46 LG:018739.2;2000SEP08555 641 forwardTM N out
3
47 LG;302915.b:2000SEP08127 207 forwardTM N in
1
47 LG;302915.b;2000SEP08330 404 forwardTM N out
3
47 LG:302915.6:2000SEP08501 575 forwardTM N out
3
47 LG:302915.6:2000SEP08630 716 forwardTM N out
3
48 LG:404418.3:2000SEP086l 702 forwardTM N out
b 1
48 LG:404418.3;2000SEP08479 565 forwardTM N out
2
48 LG:404418.3:2000SEP08626 682 forwardTM N out
2
49 LG;374853.2:2000SEP08235 312 forwardTM N out
1
49 LG:374853.2:2000SEP08913 999 forwardTM N out
1
49 LG;374853.2;2000SEP0812761362 forwardTM N out
1
49 LG:374853.2:2000SEP0813841437 forwardTM N out
1
49 LG:374853.2;2000SEP0816061692 forwardTM N out
1
49 LG;374853.2:2000SEP08122 208 forwardTM N in
2
49 LG:374853.2:2000SEP08371 439 forwardTM N in
2
49 LG;374853.2:2000SEP0811211183 forwardTM N in
2
49 LG:374853.2;2000SEP0812081270 forwardTM N in
2
49 LG:374853.2;2000SEP08198 284 forwardTM N in
3
49 LG:374853.2:2000SEP08381 467 forwardTM N in
3
49 LG;374853.2;2000SEP0813111397 forward' TM N in
3
50 LG;228930.1:2000SEP08697 750 forwardTM N out
1
50 LG;228930.1:2000SEP08111 170 forwardTM N in
3
50 LG;228930.1;2000SEP08564 650 forwardTM N in
3
50 LG;228930.1:2000SEP08858 941 forwardTM N in
3
51 LG:273593.b;2000SEP08343 429 forwardTM
1
51 LG:273593.b:2000SEP08707 763 forwardTM N out
2
52 LG;008215.1;2000SEP08181 267 forwardTM N out
1
52 LG:008215.1:2000SEP08583 663 forwardTM N out
1
52 LG:008215.1;2000SEP0811231209 forwardTM N out
1
52 LG:008215.1:2000SEP0814041466 forwardTM N out
3
53 LG:337160.1:2000SEP08844 930 forwardTM N out
1
53 LG:337160.1:2000SEP08928 990 forwardTM N out
1
53 LG:337160.1:2000SEP0810751143 forwardTM N out
1
53 LG:337160.1:2000SEP0813541437 forwardTM N out
1
53 LG:337160.1:2000SEP08119 193 forwardTM N in
2
53 LG:3371b0.1:2000SEP08275 346 forwardTM N in
2
53 LG;337160.1:2000SEP08491 577 forwardTM N in
2
53 LG:337160.1:2000SEP0810371120 forwardTM N in
2
53 LG;337160.1;2000SEP0810141100 forwardTM N out
3
54 LG:395063.1:2000SEP08331 387 forwardTM N in
1
54 LG:395063.1:2000SEP08517 603 forwardTM N in
1
54 LG;395063.1;2000SEP08748 834 forwardTM N in
1
54 LG:395063.1;2000SEP08850 912 forwardTM N in
1
54 LG;395063.1;2000SEP08982 1068 forwardTM N in
1
54 LG:3950b3.1;2000SEP0811111197 forwardTM N in
_ - 1
_ _- ._
78~
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID StartStopFrame Domain TypeTopology
NO:
54 LG;395063.1:2000SEP081882 1929forwardTM N in
1
54 LG;395063.1:2000SEP082752 2838forwardTM N in
1
54 LG:3950b3.1:2000SEP08338 424 forwardTM N out
2
54 LG;395063.1;2000SEP08464 526 forwardTM N out
2
54 LG:395063.1;2000SEP08557 619 forwardTM N out
2
54 LG:395063.1:2000SEP08683 769 forwardTM N out
2
54 LG:395063.1;2000SEP08860 931 forwardTM N out
2
54 LG:395063.1;2000SEP081121 1207forwardTM N out
2
54 LG;395063.1:2000SEP081265 1351forwardTM N out
2
54 LG:3950b3.1:2000SEP081439 1519forwardTM N out
2
54 LG:395063.1;2000SEP081571 1657forwardTM N out
2
54 LG:395063.1:2000SEP081901 1987forwardTM N out
2
54 LG;395063.1;2000SEP082240 2290forwardTM N out
2
54 LG:395063.1:2000SEP082405 2491forwardTM N out
2
54 LG:395063.1;2000SEP081122 1199forwardTM
3
54 LG:3950b3.1;2000SEP081446 1532forwardTM
3
54 LG:395063.1;2000SEP081590 1676forwardTM
3
54 LG:3950b3.1;2000SEP081800 1883forwardTM
3
54 LG:395063.1;2000SEP082382 2465forwardTM
3
54 LG:395063.1:2000SEP082796 2846forwardTM
3
55 LG:979069.4:2000SEP0810 78 forwardTM N in
1
55 LG:979069.4;2000SEP08196 279 forwardTM N in
1
55 LG:9790b9.4:2000SEP08889 969 forwardTM N in
1
55 LG:979069.4;2000SEP081015 1086forwardTM N in
1
55 LG:979069.4:2000SEP081093 1179forwardTM N in
1
55 LG;9790b9.4;2000SEP081441 1515forwardTM N in
1
55 LG;979069.4;2000SEP081783 1860forwardTM N in
1
55 LG;979069.4:2000SEP08602 664 forwardTM N out
2
55 LG:979069.4;2000SEP08680 742 forwardTM N out
2
55 LG;979069.4:2000SEP081424 1492forwardTM N out
2
55 LG:9790b9.4;2000SEP081757 1843forwardTM N out
2
55 LG;9790b9.4:2000SEP081871 1957forwardTM N out
2
55 LG:9790b9.4:2000SEP08339 419 forwardTM N out
3
55 LG;979069.4;2000SEP08429 491 forwardTM N out
3
55 LG;9790b9.4;2000SEP08507 569 forwardTM N out
3
55 LG:979069.4:2000SEP08594 674 forwardTM N out
3
55 LG:979069.4;2000SEP081422 1472forwardTM N out
3
55 LG;9790b9.4:2000SEP081863 19i forwardTM N out
9 3
56 LG;34bbb3.5;2000SEP08457 513 forwardTM
1
57 LG;347615.1:2000SEP0852 123 forwardTM
1
57 LG;347615.1:2000SEP08144 216 forwardTM
3
58 LG:1397067.1;2000SEP08401 484 forwardTM N in
2
58 LG:1397067.1:2000SEP08456 524 forwardTM N out
3
58 LG:1397067.1;2000SEP08660 746 forwardTM N out
3
59 LG:120675.1;2000SEP08301 372 forwardTM N in
1
59 LG:120b75.1;2000SEP08559 630 forwardTM N in
1~
59 LG;120b75.1:2000SEP08668 754 forwardTM
2
59 LG:120b75.1:2000SEP08758 841 forwardTM
_____. 2
_ - _______
_ .
79
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template iD Start Frame Domain TypeTopology
NO: Stop
59 LG;120675.1;2000SEP081316 1372forwardTM
2
60 LG:420050.18;2000SEP08112 198 forwardTM N out
1
60 LG;420050.18:2000SEP08205 291 forwardTM ~ N out
1
60 LG:420050.18:2000SEP08155 241 forwardTM N out
2
60 LG:420050.18;2000SEP08413 472 forwardTM N out
2
60 LG:420050.18:2000SEP08210 290 forwardTM N out
3
6l LG;220495.3;2000SEP08208 294 forwardTM N out
1
6l LG:220495.3:2000SEP08502 582 forwardTM N out
1
61 LG;220495.3;2000SEP08631 717 forwardTM N out
1
61 LG:220495.3:2000SEP08790 846 forwardTM N out
1
61 LG;220495.3;2000SEP08865 930 forwardTM N out
1
61 LG:220495.3;2000SEP081432 1518forwardTM N out
1
6l LG:220495.3:2000SEP081663 1749forwardTM N out
1
6l LG;220495.3;2000SEP081801 1866forwardTM N'out
1
61 LG:220495.3:2000SEP081918 1977forwardTM N out
1
6l LG;220495.3;2000SEP08956 1030forwardTM N out
2
6l LG;220495.3:2000SEP081439 1501forwardTM N out
2
61 LG:220495.3:2000SEP081526 1588forwardTM N out
2
6l LG:220495.3:2000SEP081631 1699forwardTM N out
2
61 LG:220495.3:2000SEP081760 1843forwardTM N out
2
6l LG:220495.3:2000SEP081862 1945forwardTM N out
2
61 LG;220495.3:2000SEP08486 536 forwardTM
3
6l LG:220495.3:2000SEP08618 677 forwardTM
3
61 LG;220495.3;2000SEP08702 779 forwardTM
3
6i LG;220495.3:2000SEP08843 926 forwardTM
3
61 LG:220495.3;2000SEP08984 1070forwardTM
3
6l LG;220495.3:2000SEP081449 1499forwardTM
3
61 LG;220495,3:2000SEP081545 1619forwardTM
3
61 LG:220495.3;2000SEP081866 1928forwardTM
3
62 LG;274551.1:2000SEP0881 152 forwardTM N out
3
62 LG:274551.1:2000SEP08216 269 forwardTM N out
3
63 LG:429b58.27;2000SEP08373 432 forward~ TM N out
1
63 LG:429658.27:2000SEP08197 259 forwardTM N out
2
63 LG;429b58.27;2000SEP08368 430 forwardTM N out
2
64 LG:246194.18;2000SEP08718 804 forwardTM N out
1
64 LG;246194.18:2000SEP081816 1890forwardTM N out
1
64 LG;246194.18;2000SEP08716 763 forwardTM N in
' 2
64 LG:246194.18:2000SEP081793 1879forwardTM N in
2
64 LG;246194.18:2000SEP08726 782 forwardTM N in
3
64 LG;24b194.18;2000SEP081830 1883forwardTM N in
3
65 LG:000874.1:2000SEP08151 237 forwardTM N in
1
65 LG;000874.1:2000SEP081738 1824forwardTM N in
1
65 LG:000874.1:2000SEP081849 1935forwardTM N in
1
65 LG;000874.1:2000SEP08170 256 forwardTM N out
2
65 LG;000874.1:2000SEP081991 2047forwardTM N out
2
65 LG;000874.1:2000SEP082078 2155forwardTM N out
2
65 LG;000874.1:2000SEP08168 233 forwardTM N in
3
65 LG:000874.1:2000SEP08627 713 forwardTM N in
_ _ _ 3
____
g0
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain Topology
NO; Stop Type
65 LG:000874.1;2000SEP081383 1469forwardTM N in
3
65 LG:000874.1:2000SEP082109 2162forwardTM N in
3
66 LG:239967.7:2000SEP08136 189 forwardTM N out
1
67 LG;238388.1:2000SEP08544 618 forwardTM N out
1
67 LG:238388.1:2000SEP081477 1536forwardTM N out
1
67 LG;238388.1:2000SEP081975 2028forwardTM N out
1
67 LG:238388.1:2000SEP082029 2100forwardTM N out
1
67 LG:238388.1;2000SEP082275 2346forwardTM N out
1
67 LG;238388.1:2000SEP082377 2463forwardTM N out
1
67 LG;238388.1:2000SEP082734 2790forwardTM N out
1
67 LG:238388.1:2000SEP082803 2889forwardTM N out
1
67 LG:238388.1;2000SEP082998 3084forwardTM N out
1
67 LG:238388.1:2000SEP083118 3204forwardTM N out
1
67 LG:238388.1:2000SEP083265 3351forwardTM N out
1
67 LG:238388.1:2000SEP083415 3489forwardTM N out
1
67 LG:238388.1:2000SEP083550 3612forwardTM N out
1
67 LG:238388.1;2000SEP083664 3726forwardTM N out
1
67 LG:238388.1;2000SEP081337 1399forwardTM N out
2
67 LG:238388.1:2000SEP081727 1804forwardTM N out
2
67 LG:238388.1;2000SEP081883 1969forwardTM N out
2
67 LG:238388.1:2000SEP082003 2065forwardTM N out
2
67 LG;238388.1:2000SEP082084 2146forwardTM N out
2
67 LG:238388.1;2000SEP082459 2545forwardTM N out
2
67 LG:238388.1:2000SEP082600 2686forwardTM N out
2
67 LG;238388.1:2000SEP082714 2785forwardTM N out
2
67 LG:238388.1:2000SEP082837 2923forwardTM N out
2
67 LG;238388.1:2000SEP082945 3031forwardTM N out
2
67 LG:238388.1;2000SEP083176 3256forwardTM N out
2
67 LG:238388.1:2000SEP083308 3394forwardTM N out
2
67 LG:238388.1:2000SEP083545 3622forwardTM N out
2
67 LG:238388.1:2000SEP081473 1553forwardTM
3
b7 LG:238388.1:2000SEP081755 1805forwardTM
3
67 LG:238388.1;2000SEP081860 1928forwardTM
3
b7 LG;238388.1;2000SEP082232 2318forwardTM
3
67 LG:238388.1;2000SEP082634 2720forwardTM
3
67 LG:238388.1;2000SEP082766 2834forwardTM
3
67 LG:238388.1:2000SEP082877 2951forwardTM
3
67 LG:238388.1;2000SEP083021 3092forwardTM
3
b7 LG:238388.1:2000SEP083369 3452forwardTM
3
67 LG:238388.1:2000SEP083636 3719forwardTM
3
68 LG:233b74.4:2000SEP083175 3261forwardTM N in
1
68 LG:233674.4:2000SEP083526 3612forwardTM N in
1
68 LG:233674.4:2000SEP081826 1885forwardTM N out
2
68 LG:233674.4:2000SEP083285 3365forwardTM N out
3
68 LG:233674.4;2000SEP083474 3560forwardTM N out
3
69 LG:411327.2:2000SEP08226 312 forwardTM N out
1
69 LG:411327.2:2000SEP081327 1407forwardTM N out
1
69 LG:411327.2:2000SEP08239 322 forwardTM N out
2
81
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain TypeTopology
NO. Stop
69 ~ LG;411327.2;2000SEP08794 865forward TM N out
2
69 LG:411327.2:2000SEP08588 650forward TM N in
3
69 LG:411327.2:2000SEP08666 728forward TM N in
3
69 LG;411327.2;2000SEP08744 806forward TM N in
3
69 LG;411327.2:2000SEP08828 914forward TM N in
3
69 LG;411327.2:2000SEP081557 1643forward TM N in
3
69 LG:411327.2:2000SEP081749 1829forward TM N in
3
69 ' LG:411327.2;2000SEP081842 1925forward TM N in
3
70 LG:1327310.1:2000SEP08193 258forward TM N in
1
71 LG:242019.13;2000SEP08367 453forward TM N out
1
71 LG:242019.13:2000SEP08402 452forward TM N out
3
72 LG:012432.12:2000SEP08319 405forward TM N in
1
72 LG:O T 2432. T 526 612forward TM N in
2:2000SEP08 1
72 LG:012432.12:2000SEP08290 376forward TM N in
2
72 LG;Oi2432.T2:2000SEP08506 565forward TM N in
2
72 LG:012432.12;2000SEP08677 757forward TM N in
2
72 LG;012432.12:2000SEP08642 695forward TM N out
3
73 LG:257088.9;2000SEP081231 1302forward TM N out
T
73 LG;257088.9;2000SEP081339 1425forward TM N out
1
73 LG:257088.9:2000SEP08152 229forward TM
2
73 LG:257088.9:2000SEP08656 739forward TM
2
73 LG:257088.9:2000SEP0872 143forward TM
3
73 LG;257088.9;2000SEP08630 692forward TM
3
73 LG:257088.9:2000SEP08939 1010forward TM
3
74 LG;997505.5;2000SEP081036 1089forward TM N out
1
74 LG:997505.5:2000SEP081092 1142forward TM N in
3
74 LG:997505.5;2000SEP081224 1271forward TM N in
3
75 LG:481436.2;2000SEP0810 60 forward TM N out
1
75 LG;481436.2;2000SEP08256 324forward TM N out
1
75 LG:481436.2:2000SEP08430 516forward TM N out
1
75 LG;481436.2;2000SEP08562 648forward TM N out
1
75 LG:48143b.2:2000SEP08775 834forward TM N out
1
75 LG;481436.2:2000SEP081060 1122forward TM N out
1
75 LG:481436.2:2000SEP08T 1209forward TM N out
T47 1
75 LG;481436.2:2000SEP081414 1500forward TM N out
1
75 LG:481436.2:2000SEP081522 1608forward TM N out
1
75 LG;481436.2;2000SEP08230 316forward TM N in
2
75 LG:481436.2;2000SEP08359 436forward TM N in
2
75 LG:481436.2:2000SEP08536 607forward TM N in
2
75 LG;481436.2;2000SEP08635 697forward TM N in
2
75 LG:481436.2:2000SEP08710 772forward TM N in
2
75 LG;481436.2;2000SEP08785 847forward TM N in
2
75 LG:481436.2:2000SEP081385 1471forward TM N in
2
75 LG:481436.2;2000SEP081502 1570forward TM N in
2
75 LG;481436.2:2000SEP08276 353forward TM
3
75 LG:481436.2;2000SEP08522 608forward TM
3
75 LG;481436.2:2000SEP08840 926forward TM
3
75 LG:481436.2;2000SEP081383 1469forward TM
- ____ 3
__._
_
g2
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain TypeTopology
NO: Stop
75 LG;481436.2:2000SEP081500 1586forwardTM
3
76 LG:247776.i4:2000SEP08932 985 forwardTM N out
2
77 LG:008606.14:2000SEP08414 500 forwardTM N in
3
77 LG:008606.14:2000SEP08540 587 forwardTM N in
3
77 LG:008606.14:2000SEP081146 1199forwardTM N in
3
78 LG;985092.3;2000SEP0824 110 forwardTM N out
3
79 LG;236b49.7:2000SEP08370 456 forwardTM N in
1
79 LG:236649.7;2000SEP08377 463 forwardTM N out
2
79 LG;236649.7:2000SEP08366 440 forwardTM N in
3
80 LG:245014.2:2000SEP081345 1407forwardTM N in
1
80 LG:245014.2;2000SEP081426 1488forwardTM N in
1
80 LG:245014.2:2000SEP08464 541 forwardTM N in
2
80 LG:245014.2;2000SEP08599 655 forwardTM N in
2
80 LG;245014.2:2000SEP08731 817 forwardTM N in
2
80 LG:245014.2:2000SEP081325 1408forwardTM N in
2
80 LG:245014.2;2000SEP08252 338 forwardTM N out
3
80 LG;245014.2:2000SEP081335 1421forwardTM N out
3
81 LG:170754.4:2000SEP08244 330 forwardTM N out
1
81 LG:170754.4;2000SEP0871 157 forwardTM N in
2
81 LG:170754.4:2000SEP08206' 292 forwardTM N in
2
81 LG;170754.4:2000SEP08585 635 forwardTM N in
3
82 LG:988028.1:2000SEP08268 354 forwardTM N out
1
83 LG;427997.b:2000SEP08148 222 forwardTM ' N out
1
83 LG;427997.b:'2000SEP08739 822 forwardTM N out
1
83 LG:427997.b:2000SEP08859 939 forwardTM N aut
1
83 LG;427997.b:2000SEP08i 1395forwardTM N out
309 1
83 LG:427997.b:2000SEP081609 1695forwardTM N out
1
83 LG:427997.b:2000SEP081726 1812forwardTM N out
1
83 LG;427997.b;2000SEP082107 2187forwardTM N out
1
83 LG:427997.b:2000SEP08134 220 forwardTM N in
2
83 LG;427997.6:2000SEP08752 829 forwardTM N in
2
83 LG;427997.6:2000SEP081229 1309forwardTM N in
2
83 LG;427997.b:2000SEP081487 1540forwardTM N in
2
83 LG:427997.b;2000SEP081619 1687forwardTM N in
2
83 LG:427997.b:2000SEP081715 1789forwardTM N in
2
83 LG:427997.b:2000SEP08150 236 forwardTM N out
3
83 LG:427997.b:2000SEP08681 743 forwardTM N out
3
83 LG:427997.b:2000SEP08759 821 forwardTM N out
3
83 LG;427997.b:2000SEP081074 1142forwardTM N out
3
83 LG:427997.b;2000SEP081 1256forwardTM N out
i 3
85
83 LG:427997.b:2000SEP081449 1520forwardTM N out
3
83 LG:427997.b:2000SEP081740 1826forwardTM N out
3
84 LG:464206.1;2000SEP084 81 forwardTM N out
1
84 LG:464206.1:2000SEP0811 82 forwardTM N out
2
84 LG;464206.1:2000SEP08105 170 forwardTM N out
3
85 LG:1400108.1:2000SEP08283 351 forwardTM N out
1
85 LG:1400108.1;2000SEP08373 447 forwardTM N out
1
85 LG:1400108.1:2000SEP08598 684 forwardTM N out
1
83
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID StartStopFrame Domain TypeTopology
NO;
85 LG:1400108.1:2000SEP08724 786 forwardTM N out
1
85 LG:1400108.1:2000SEP08802 864 forwardTM N out
1
85 LG:1400108.1:2000SEP08871 948 forwardTM N out
1
85 LG;1400108.1:2000SEP0892 175 forwardTM N out
2
85 LG:1400108.1:2000SEP08293 355 forwardTM N out
2
85 LG:1400108.1:2000SEP08368 430 forwardTM N out
2
85 LG:1400108.1:2000SEP08587 649 forwardTM N out
2
85 LG:1400108.1:2000SEP08662 724 forwardTM N out
2
85 LG;1400108.1:2000SEP08785 862 forwardTM N out
2
85 LG:1400108.1;2000SEP08884 970 forwardTM N out
2
85 LG:1400108, i 300 37 forwardTM N out
:2000SEP08 i 3
85 LG:1400108.1;2000SEP08393 446 forwardTM N out
3
85 LG:1400108.1:2000SEP08651 737 forwardTM N out
3
85 LG:1400108.1;2000SEP08765 827 forwardTM N out
3
85 LG:1400108.1:2000SEP08852 914 forwardTM N out
3
86 LG:254531.1;2000SEP0831 111 forwardTM N in
1
86 LG;254531.1:2000SEP08277 327 forwardTM N in
1
86 LG:254531.1:2000SEP0853 133 forwardTM N out
2
86 LG:254531.1:2000SEP08548 634 forwardTM N out
2
86 LG;254531.1;2000SEP0860 110 forwardTM N in
3
86 LG;25453 i , i 231 305 forwardTM N in
:2000SEP08 3
86 LG:254531.1;2000SEP08495 581 forwardTM N in
3
86 LG:254531.1;2000SEP08687 761 forwardTM N in
3
87 LG;1101317.1:2000SEP08607 678 forwardTM N out
1
87 LG:1101317.1:2000SEP081186 1236forwardTM N out
1
87 LG;1101317.1:2000SEP082482 2568forwardTM N out
1
87 LG;1101317.1:2000SEP082825 2899forwardTM N out
2
87 LG:1101317.1;2000SEP081023 1091forwardTM N out
3
87 LG;1101317.1;2000SEP081194 1271forwardTM N out
3
88 LG:1074728.6:2000SEP081 250 forwardTM N in
b4 2
88 LG: T 074728.6;2000SEP08437 523 forwardTM N in
2
89 LG:1081684.1:2000SEP08124 210 forwardTM
1
89 LG:1081684.1:2000SEP08256 330 forwardTM
1
89 LG;1081684.1;2000SEP08361 423 forwardTM
1
89 LG:1081 b84.1:2000SEP08460 522 forwardTM
1
89 LG:1081684.1:20005EP08179 265 forwardTM N in
2
89 LG:1081684.1;2000SEP08626 673 forwardTM N in
2
89 LG;1081684.1;2000SEP08147 233 forwardTM N out
3
89 LG;1081684.1:2000SEP08273 341 forwardTM N out
3
89 LG:1081684.1:2000SEP08438 509 forwardTM N out
3
90 LG:1076520.1:2000SEP0870 132 forwardTM N out
1
90 LG;107b520.1:2000SEP08151 213 forwardTM N out
1
90 LG:1076520.1;2000SEP08232 294 forwardTM N out
1
90 LG:1076520.1:2000SEP08310 378 forwardTM N out
1
90 LG: i 076520.1:2000SEP08550 636 forwardTM N out
1
90 LG:1076520.1;2000SEP08760 828 forwardTM N out
1
91 LG:1079477.1:2000SEP0810 75 forwardTM N out
1
91 LG:1079477.1:2000SEP08115 189 forwardTM N out
_ _ 1
_
g4
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template !D Start Frame Domain TypeTopology
NO; Stop
91 LG:1079477.1:2000SEP0826 88 forwardTM N out
2
91 LG:T079477.1:2000SEP08122 184 forwardTM N out
2
91 LG:1079477.1:2000SEP0827 92 forwardTM N in
3
92 LG:107b2b9.1:2000SEP08299 358 forwardTM N out
2
92 LG:1076269.1:2000SEP08443 520 forwardTM N out
2
93 LG; T 087195.1;2000SEP08974 1045forwardTM N in
2
93 LG:1087195.1:2000SEP08945 1031forwardTM N out
3
93 LG:1087195.1:2000SEP08T 1271forwardTM N out
185 3
94 LG:002588.7:2000SEP0828 114 forwardTM N out
1
94 LG:002588.7:2000SEP08130 186 forwardTM N out
1
94 LG:002588.7:2000SEP08349 432 forwardTM N out
1
94 LG:002588.7:2000SEP08436 522 forwardTM N out
1
94 LG:002588.7:2000SEP08613 690 forwardTM N out
1
94 LG:002588.7:2000SEP0838 100 forwardTM N out
2
94 LG:002588.7:2000SEP08T T forwardTM N out
37 99 2
94 LG;002588.7:2000SEP08374 436 forwardTM N out
2
94 LG;002588.7;2000SEP08458 520 forwardTM N out
2
94 LG:002588.7;2000SEP08542 604 forwardTM N out
2
94 LG;002588.7:2000SEP08623 709 forwardTM N out
2
94 LG:002588.7:2000SEP0830 11 forwardTM N out
b 3
94 LG;002588.7;2000SEP08150 224 forwardTM N out
3
94 LG:002588.7;2000SEP08423 494 forwardTM N out
3
94 LG:002588.7;2000SEP08546 632 forwardTM N out
3
94 LG;002588.7;2000SEP08759 842 forwardTM N out
3
95 LG:1079470.6:2000SEP0825 111 forwardTM N in
1
95 LG;1079470.b;2000SEP08208 294 forwardTM N in
. 1
95 LG;1079470.b:2000SEP08448 531 forwardTM N in
1
95 LG;1079470.6;2000SEP081021 1107forwardTM N in
1
95 LG;1079470.6;2000SEP0856 142 forwardTM N out
2
95 LG:1079470.6:2000SEP08209 295 forwardTM N out
2
95 LG:1079470.6;2000SEP08389 442 forwardTM N out
2
95 LG;1079470.b:2000SEP086T 691 forwardTM N out
7 2
95 LG;1079470.b:2000SEP08863 946 forwardTM N out
2
95 LG:1079470.6:2000SEP081016 1078forwardTM N out
2
95 LG;1079470.6;2000SEP081097 1159forwardTM N out
2
95 LG:1079470.b:2000SEP08378 440 forwardTM N out
3
95 LG;1079470.b;2000SEP08474 536 forwardTM N out
3
95 LG;1079470.6:2000SEP08744 824 forwardTM N out
3
96 LG;345705.3;2000SEP08187 270 forwardTM N in
1
97 LG:1083654.1;2000SEP081066 1152forwardTM N out
1
97 LG;1083b54.1;2000SEP082836 2898forwardTM N out
1
97 LG:1083b54.1:2000SEP082917 2979forwardTM N out
1
97 LG:1083b54.1:2000SEP083337 3423forwardTM N out
1
97 LG:1083b54.1:2000SEP08197 283 forwardTM N out
2
97 LG;1083b54.1:2000SEP08650 736 forwardTM N out
2
97 LG:1083b54.1:2000SEP081049 1135forwardTM N out
2
97 LG:1083b54.1:2000SEP082681 2767forwardTM N out
2
97 LG; T 083b54.1:2000SEP082975 3061forwardTM N out
2
SJ
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain TypeTopology
NO: Stop
97 LG:1083654.1:2000SEP0831613247forward TM N out
2
97 LG:1083654.1:2000SEP08765 827 forward TM N in
3
97 LG:1083654.1:2000SEP0810471133forward TM N in
3
97 LG:1083654.1:2000SEP0825922666forward TM N in
3
97 LG:1083654.1:2000SEP0827332819forward TM N in
3
97 LG:1083b54,1:2000SEP0829253011forward TM N in
3
97 LG:1083b54.1:2000SEP0831293215forward TM N in
3
98 LG:198782,3:2000SEP08814 900 forward TM
1
98 LG:198782.3:2000SEP0813391392forward TM
1
98 LG:198782,3:2000SEP0816781764forward TM
1
98 LG:198782.3:2000SEP08233 283 forward TM N in
2
98 LG:198782.3:2000SEP08830 892 forward TM N in
, 2
98 LG:198782,3:2000SEP0816551741forward TM N in
2
98 LG:198782.3:2000SEP0820752137forward TM N in
2
98 LG:198782,3:2000SEP0826392695forward TM N in
2
98 LG:198782,3:2000SEP08792 878 forward TM N in
3
98 LG:198782.3:2000SEP0820402111forward TM N in
3
98 LG:198782,3:2000SEP0825772651forward TM N in
3
99 LG:981076.2:2000SEP0819 81 forward TM N out
1
99 LG:981076.2:2000SEP08409 495 forward TM N out
1
99 LG:981076,2:2000SEP08538 603 forward TM N out
1
99 LG:981076.2:2000SEP08437 523 forward TM N in
2
99 LG:981076.2:2000SEP08387 449 forward TM N in
3
100 LG:212023.3:2000SEP08265 342 forward TM N out
1
100 LG:212023,3:2000SEP0811291215forward TM N out
1
100 LG:212023.3:2000SEP08497 583 forward TM N in
2
100 LG:212023,3:2000SEP0811811231forward TM N in
2
100 LG:212023,3:2000SEP08501 587 forward TM N in
3
100 LG:212023.3:2000SEP08627 698 forward TM N in
3
100 LG:212023.3:2000SEP08732 794 forward TM N in
3
100 LG:212023,3:2000SEP08822 884 forward TM N in
3
100 LG:212023.3:2000SEP0810111064forward TM N in
3
101 LG:977929.3:2000SEP08115 177 forward TM N out
1
101 LG:977929.3:2000SEP08196 258 forward TM N out
1
102 LG:201936.b:2000SEP08445 519 forward TM N out
1
102 LG:201936,6:2000SEP0892 178 forward TM N out
2
102 LG:201936.6:2000SEP08449 511 forward TM N out
2
102 LG:201936.6:2000SEP08521 583 forward TM N out
2
102 LG:207 93b.6:2000SEP08641 709 forward TM N out
2
102 LG:201936,b:2000SEP08719 802 forward TM N out
2
102 LG:20193b,b:2000SEP08279 326 forward TM N out
3
103 LG:205642.1:2000SEP08172 237 forward TM N in
1
103 LG:205642.1:2000SEP08674 751 forward TM N out
2
103 LG:205642.1:2000SEP08752 832 forward TM N out
2
103 LG:205642.1:2000SEP08935 1021forward TM N out
2
103 LG:205642.1:2000SEP08117 197 forward TM N in
3
103 LG:205642.1:2000SEP08717 797 forward TM N in
3
104 LG:339653.b:2000SEP0828 105 forward TM N in
1
86
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain TypeTopology
NO: Stop
105 LG:978587.4;2000SEP08394 474 forwardTM N out
1
105 LG:978587.4;2000SEP0811 64 forwardTM N out
2
105 LG:978587.4:2000SEP08506 592 forwardTM N out
2
105 LG;978587.4:2000SEP0812 68 forwardTM N out
3
106 LG;21 b848, i 309 395 forwardTM N in
7:2000SEP08 3
107 LG;219502.1:2000SEP08109 195 forwardTM N out
1
i 07 LG:219502, i ;2000SEP082 289 forwardTM
. i 2
5
107 LG;219502,1:2000SEP08740 802 forwardTM
2
107 LG;2i9502.1;2000SEP08228 293 forwardTM N out
3
107 LG:219502,1:2000SEP08378 431 forwardTM N out
3
108 LI:334211.1;2000SEP0879 141 forwardTM N out
1
108 LI:334211.1:2000SEP0880 142 forwardTM N out
2
108 LI:334211.1;2000SEP08686 739 forwardTM N out
2
108 LI;334211,1:2000SEP0890 146 forwardTM N out
3
108 LI:334211.1;2000SEP0810831133 forwardTM N out
3
109 LI;231024,2;2000SEP08304 357 forwardTM
1
109 LI;231024.2;2000SEP0813601446 forwardTM
1
109 LI:231024.2;2000SEP085b3 628 forwardTM N in
2
109 LI;231024,2:2000SEP08851 937 forwardTM N in
2
109 LI;231024.2;2000SEP0812471333 forwardTM N in
2
109 LI;231024.2;2000SEP0813701420 forwardTM N in
2
109 LI:231024,2:2000SEP0814661519 forwardTM N in
2
109 LI;231024,2:2000SEP0815 101 forwardTM N in
3
109 LI;231024.2;2000SEP0812811349 forwardTM N in
3
109 LI;231024.2;2000SEP0813531424 forwardTM N in
3
109 LI;231024.2;2000SEP0814671538 forwardTM N in
3
109 LI;231024,2:2000SEP0815781625 forwardTM N in
3
110 LI:228425.5;2000SEP08178 255 forwardTM N out
1
110 L1:228425,5;2000SEP08385 435 forwardTM N out
1
110 LI;228425.5;2000SEP08778 864 forwardTM N out
1
110 LI;228425,5;2000SEP08401 487 forwardTM N in
2
110 LI:228425.5;2000SEP08644 715 forwardTM N in
2
110 LI:228425.5;2000SEP08770 850 forwardTM N in
2
i 10 Li;228425,5:2000SEP08147 233 forwardTM N in
3
110 LI:228425,5;2000SEP08267 338 forwardTM N in
3
110 LI:228425.5;2000SEP08381 467 forwardTM N in
3
110 LI:228425,5;2000SEP08624 680 forwardTM N in
3
111 LI;034493,1:2000SEP08319 381 forwardTM N in
1
111 LI;034493.1:2000SEP08421 483 forwardTM N in
1
111 LI:034493.1:2000SEP08323 391 forwardTM N out
2
111 LI:034493,1;2000SEP08410 493 forwardTM N out
2
11 i LI:034493.1:2000SEP08617 703 forwardTM N out
2
i 11 LI:034493, i ;2000SEP08285 3b8 forwardTM N in
3
111 LI:034493.1;2000SEP08408 470 forwardTM N in
3
11 i LI;034493.1:2000SEP08492 554 forwardTM N in
3
111 LI:034493.1;2000SEP08576 638 forwardTM N in
3
i 11 LI:034493,1:2000SEP08759 82i forwardTM N in
3
111 LI:034493.1;2000SEP08858 920 forwardTM N in
_ ~ _ 3
_ _ _-
g7
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain TypeTopology
NO: Stop
112 LI:336218.1:2000SEP0829 85 forwardTM N in
2
112 LI:336218.1:2000SEP08143 229 forwardTM N in
2
112 LI:33b218,1:2000SEP08278 349 forwardTM N in
2
113 LI:235891.3:2000SEP08380 436 forward, TM N out
2
114 LI:344094.1:2000SEP0837 123 forwardTM N in
1
114 LI:344094.1:2000SEP08223 306 forwardTM N in
1
114 LI:344094,1:2000SEP08493 579 forwardTM N in
1
114 LI:344094.1:2000SEP08631 717 forwardTM N in
1
114 LI:344094,1:2000SEP0844 1 forwardTM
Ob 2
114 LI:344094,1:2000SEP08122 184 forwardTM
2
114 LI:344094.1;2000SEP08218 280 forwardTM
2
114 LI:344094.1:2000SEP08521 607 forwardTM
2
114 LI:344094,1:2000SEP0824 110 forwardTM N in
3
114 LI:344094.1:2000SEP08210 287 forwardTM N in
3
114 LI:344094.1:2000SEP08534 620 forwardTM N in
3
115 LI:399945.2:2000SEP08546 605 forwardTM
3
116 LI:051849,1:2000SEP08175 261 forwardTM N out
1
117 LI:238379,3:2000SEP08450 524 forwardTM N in
3
118 LI:352190.8:2000SEP08194 241 forwardTM N out
2
119 LI:432120.1:2000SEP08681 767 forwardTM
3
120 LI:055461.1:2000SEP08349 435 forwardTM N in
1
120 LI:055461,1:2000SEP0813451407 forwardTM N in
1
120 LI:055461.1:2000SEP08146 208 forwardTM N out
2
120 LI:055461.1:2000SEP08242 304 forwardTM N out
2
120 LI:055461,1:2000SEP08662 724 forwardTM N out
2
120 LI:055461.1:2000SEP0813791450 forwardTM N out
2
121 LI:197433.5:2000SEP0819 81 forwardTM N out
1
121 LI:197433.5:2000SEP08100 162 forwardTM N out
1
121 LI:197433,5:2000SEP08175 261 forwardTM N out
1
121 LI:197433.5:2000SEP08355 417 forwardTM N out
1
121 LI:197433,5:2000SEP08442 504 forwardTM ~ N
1 out
121 LI:197433.5:2000SEP08529 591 forwardTM N out
1
7 22 LI:170b04.1:2000SEP08439 516 forwardTM N out
1
122 LI:170b04,1:2000SEP08429 515 forwardTM N out
3
123 LI:205057.3:2000SEP0810 72 forwardTM N in
1
123 LI:205057.3:2000SEP08139 210 forwardTM N in
1
123 LI:205057,3:2000SEP08437 499 forwardTM N in
2
123 LI:205057.3:2000SEP08512 574 forwardTM N in
2
123 LI:205057.3:2000SEP0812 98 forwardTM N out
3
123 LI:205057.3:2000SEP08150 236 forwardTM N out
3
124 LI:233795.1:2000SEP0892 1 forwardTM N in
b6 2
124 LI:233795.1:2000SEP08108 161 forwardTM N out
3
125 LI:311197.1:2000SEP08241 318 forwardTM
1
125 LI:311197.1:2000SEP08604 690 forwardTM
1
i 25 LI:311 i 97, i 530 592 forwardTM N out
:2000SEP08 2
125 LI:311197.1:2000SEP08614 676 forwardTM N out
2
125 LI:311197.1:2000SEP08621 701 forwardTM N in
3
126 LI:441364.1:2000SEP08321 377 forwardTM N in
3
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID StartStopFrame Domain TypeTopology
NO:
i 27 LI:2i 03b7.b:2000SEP08330 4i forward TM' N in
6 3
128 LI:238194.5:2000SEP08457 543 forward TM N in
1
129 LI;039258.5:2000SEP08396 482 forward TM N in
3
130 LI:1071842.1:2000SEP08439 486 forward TM N out
1
130 LI:1071842.1;2000SEP08504 575 forward TM N out
3
131 LI:481356.3:2000SEP08580 663 forward TM N out
1
132 Ll: ) 03474.1:2000SEP0810 90 forward TM N out
1
132 LI:103474.1:2000SEP08100 1 forward TM N out
b8 1
132 LI:103474.1:2000SEP08412 462 forward TM N out
1
132 LI;103474.1:2000SEP0811 76 forward TM N out
2
132 LI:103474.1:2000SEP0815 101 forward TM N out
3
132 LI:103474.1:2000SEP08117 185 forward TM N out
3
132 LI;103474.1:2000SEP082b7 353 forward TM ~ N
3 out
133 LI:1073020.10:2000SEP086i3 699 forward TM N out
i
133 LI;1073020.10:2000SEP0811 97 forward TM N out
2
133 LI:1073020.10:2000SEP08818 880 forward TM N out
2
133 LI;1073020.10:2000SEP0812591330forward TM N out
2
133 LI;1073020.10;2000SEP08270 353 forward TM N in
3
133 LI:1073020.10:2000SEP0810081094forward TM N in
3
134 LI:000874.1;2000SEP08151 237 forward TM N in
1
134 LI;000874.1:2000SEP0817381824forward TM N in
1
134 LI:000874.1:2000SEP0818491935forward TM N in
1
134 LI:000874.1:2000SEP0821042157forward TM N in
1
134 LI:000874.1:2000SEP08170 256 forward TM N in
2
134 LI:000874.1;2000SEP08168 233 forward TM N in
3
134 LI;000874.1:2000SEP08627 713 forward TM N in
3
134 Li;000874.1:2000SEP0813831469forward TM N in
3
134 LI:000874.1;2000SEP0820372093forward TM N in
3
134 LI:000874.1:2000SEP0821242201forward TM N in
3
135 LI:037298.2;2000SEP08373 441 forward TM N in
1
135 LI:037298.2;2000SEP084b0 540 forward TM N in
1
135 LI;037298.2:2000SEP08b25 675 forward TM N in
1
135 LI:037298.2;2000SEP08715 777 forward TM N in
1
135 LI:037298.2:2000SEP08793 855 forward TM N in
1
135 LI;037298.2:2000SEP08889 951 forward TM N in
1
135 LI:037298.2:2000SEP08997 1059forward TM N in
1
135 LI;037298.2:2000SEP0811171203forward TM N in
1
135 LI:037298.2;2000SEP0812731344forward TM N in
1
135 LI:037298.2:2000SEP0815071578forward TM N in
1
135 LI:037298.2:2000SEP0817831869forward TM N in
1
135 LI:037298.2:2000SEP0810671141forward TM N in
2
135 LI:037298.2:2000SEP0814751561forward TM N in
2
135 LI:037298.2:2000SEP0825042590forward TM N in
2
135 . LI:037298.2:2000SEP08471 551 forward TM
3
135 LI:037298.2;2000SEP0810621133forward TM
3
135 LI:037298.2:2000SEP0815181583forward TM
3
135 LI:037298.2:2000SEP0817791862forward TM
3
135 LI:037298.2;2000SEP0824842537forward TM
_ 3
_
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame DomainType Topology
NO; Stop
136 LI:422901.1:2000SEP0867 132 forward TM N in
1
136 LI:422901.1:2000SEP08256 336 forward TM N in
1
136 LI:422901.1:2000SEP08550 612 forward TM N in
1
136 LI;422901.1;2000SEP08625 687 forward TM N in
1
136 LI:422901.1;2000SEP08700 762 forward TM N in
1
136 LI;422901.1;2000SEP08413 466 forward TM N out
2
136 LI;422901.1:2000SEP08617 700 forward TM N out
2
136 LI;422901.1:2000SEP0811721249forward TM N out
2
137 LI:345815.1:2000SEP08580 666 forward TM N in
1
137 LI;345815.1;2000SEP0812161278forward TM N in
1
137 LI:345815.1:2000SEP0815041590forward TM N in
1
137 LI;345815.1:2000SEP0817891875forward TM N in
1
137 LI;345815.1:2000SEP08221 289 forward TM N in
2
137 LI:345815.1:2000SEP08320 367 forward TM N in
2
137 LI:345815.1:2000SEP08413 499 forward TM N in
2
137 LI;345815.1:2000SEP08665 727 forward TM N in
2
137 LI:345815.1:2000SEP08740 802 forward TM N in
2
137 LI;345815.1;2000SEP08878 949 forward TM N in
2
137 LI;~345815.1;2000SEP0810581138forward TM N in
2
137 LI:345815.1:2000SEP0817931879forward TM N in
2
137 LI:345815.1;2000SEP08372 428 forward TM N in
3
137 LI;345815.1:2000SEP08585 671 forward TM N in
3
137 LI:345815.1:2000SEP08813 899 forward TM N in
3
137 LI:345815.1;2000SEP0811401199forward TM N in
3
137 LI:345815.1:2000SEP0812901376forward TM N in
3
137 LI;345815.1:2000SEP0814761562forward TM N in
3
137 LI;345815.1:2000SEP0816681754forward TM N in
3
137 LI;345815.1:2000SEP0817761853forward TM N in
3
138 LI;1072014.2;2000SEP08674 760 forward TM N in
2
139 L1:333138.3:2000SEP0819 96 forward TM N out
1
139 LI;333138.3:2000SEP08116 181 forward TM N out
2
139 LI:333138.3:2000SEP08272 331 forward TM N out
2
140 LI:414253.1:2000SEP08115 201 forward TM N out
1
140 LI:414253.1:2000SEP0822722358forward TM N out
1
140 LI:414253.1:2000SEP08107 193 forward TM N in
2
140 LI;414253.1:2000SEP0817271777forward TM N in
2
140 LI:414253.1;2000SEP0822852365forward TM N in
2
140 LI:414253.1:2000SEP0824112464forward TM N in
2
140 LI;414253.1:2000SEP08108 182 forward TM N out
3
140 LI:414253.1:2000SEP0823462429forward TM N out
3
141 LI;406389.1;2000SEP08106 180 forward TM
1
141 LI:406389.1:2000SEP08490 576 forward TM
1
141 LI;406389.1:2000SEP08595 681 forward TM
1
141 LI:406389.1;2000SEP08709 780 forward TM
1
142 LI:108b171.1;2000SEP08226 312 forward TM
1
142 LI:1086171.1;2000SEP08218 304 forward TM N out
2
142 LI:1086171.1:2000SEP08240 311 forward TM N out
3
142 LI:108b171.1:2000SEP08420 _479forward TM N out
3
90
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain TypeTopology
NO: Stop
143 LI;198782.4;2000SEP08235 285 forwardTM N in
1
143 LI;198782.4:2000SEP08838 924 forwardTM N in
1
143 LI:198782.4:2000SEP0816901776 forwardTM N in
1
143 LI:198782.4:2000SEP0820892175 forwardTM N in
1
143 LI:198782.4:2000SEP0826772739 forwardTM N in
1
143 LI:198782.4:2000SEP0829052988 forwardTM N in
1
143 LI;198782.4:2000SEP0831033186 forwardTM N in
1
143 LI:198782.4:2000SEP0832623348 forwardTM N in
1
143 L1:198782.4:2000SEP0836853747 forwardTM N in
1
143 LI:198782.4:2000SEP0837693831 forwardTM N in
1
143 LI;198782.4;2000SEP0838533915 forwardTM N in
1
143 LI:198782.4;2000SEP0842104275 forwardTM N in
1
143 LI:198782.4:2000SEP0844834569 forwardTM N in
1
143 LI:198782.4;2000SEP0847564842 forwardTM N in
1
143 LI;198782.4;2000SEP0848914977 forwardTM N in
1
143 LI;198782.4;2000SEP08809 895 forwardTM N out
2
143 LI;198782.4;2000SEP08i T forwardTM N out
355 408 2
143 LI:198782.4:2000SEP0825672653 forward~ TM N out
2
143 LI:198782.4:2000SEP0826602725 forwardTM N out
2
143 LI:198782.4:2000SEP0827382797 forwardTM N out
2
143 LI:198782.4;2000SEP0829182974 forwardTM N out
2
143 LI:198782.4:2000SEP0837403802 forwardTM N out
2
143 LI:198782.4;2000SEP0838303892 forwardTM N out
2
143 LI:198782.4:2000SEP0842774363 forwardTM N out
2'
143 LI:198782.4:2000SEP0844754561 forwardTM N out
2
143 LI;198782.4;2000SEP0847304816 forwardTM' N out
2
143 LI:198782.4:2000SEP0848534939 forwardTM N out
2
143 LI:198782.4:2000SEP08816 899 forwardTM N in
3
143 LI;198782.4;2000SEP0812691355 forwardTM N in
3
T 43 LI;198782.4;2000SEP0823282408 forwardTM N in
3
143 LI:198782.4:2000SEP0826762756 forwardTM N in
3
143 LI:198782.4:2000SEP0837383800 forwardTM N in
3
143 LI:198782.4:2000SEP0838223884 forwardTM N in
3
143 LI;198782.4:2000SEP0841734238 forwardTM N in
3
143 LI:198782.4:2000SEP0842694337 forwardTM N in
3
143 LI:198782.4:2000SEP0848544940 forwardTM N in
3
143 LI:198782.4;2000SEP0849565036 forwardTM N in
3
144 LI:2030279.1:2000SEP08193 279 forwardTM N out
1
144 LI:2030279.1:2000SEP08496 558 forwardTM N out
1
144 LI:2030279. T 577 639 forwardTM N out
;2000SEP08 1
144 LI;2030279.1:2000SEP08649 723 forwardTM N out
1
144 LI:2030279. T 29 79 forwardTM N in
:2000SEP08 2
144 LI:2030279.1;2000SEP0898 157 forwardTM N in
2
144 LI:2030279.1:2000SEP08179 265 forwardTM N in
2
T 44 LI:2030279. T 374 436 forwardTM N in
;2000SEP08 2
144 LI;2030279.1:2000SEP08449 511 forwardTM N in
2
144 LI:2030279.1;2000SEP08662 739 forwardTM N in
2
144 LI;2030279.1:2000SEP081118_1204 forwardTM N in
2
X31
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID StartStop Frame Domain TypeTopology
NO:
144 LI:2030279.1:2000SEP08177 263 forwardTM N in
3
144 LI:2030279.1:2000SEP08288 374 forwardTM N in
3
144 LI:2030279.1:2000SEP08456 518 forwardTM N in
3
144 LI;2030279.1;2000SEP08534 596 forwardTM N in
3
144 LI:2030279.1:2000SEP086i5 701 forwardTM N in
3
144 LI:2030279.1:2000SEP0811401190 forwardTM N in
3
144 LI:2030279.1:2000SEP0813561424 forwardTM N in
3
145 LI:1018424.3:2000SEP08107 175 forwardTM N out
2
146 LI:130969.1:2000SEP08973 1044 forwardTM N out
1
146 LI:130969.1;2000SEP0826 91 forwardTM N out
2
146 LI:1309b9.1:2000SEP08458 544 forwardTM N out
2
146 LI;130969.1:2000SEP08108 167 forwardTM N out
3
147 LI:286246.2:2000SEP08373 447 forwardTM N in
1
147 LI:286246.2:2000SEP0817 1185 forwardTM N in
26 1
147 LI:286246.2;2000SEP0810971156 forwardTM N out
2
147 LI:28b246.2:2000SEP0815141576 forwardTM N out
2
147 LI:28b246.2;2000SEP0824262512 forwardTM N out
2
147 LI:286246.2:2000SEP08345 431 forwardTM N out
3
147 LI:28b246.2;2000SEP08528 590 forwardTM N out
3
147 LI:28b246.2:2000SEP0811641217 forwardTM N out
3
148 LI:001527.1:2000SEP08658 744 forwardTM N out
1
148 LI:001527.1;2000SEP08916 993 forwardTM N out
1
148 . LI:001527.1;2000SEP08335 421 forwardTM N out
2
148 LI:001527.7:2000SEP08566 652 forwardTM N out
2
148 LI:001527.1;2000SEP08686 766 ~ forwardTM N out
2
148 LI:001527.1:2000SEP08860 946 forwardTM N out
2
148 LI:001527.1:2000SEP08968 1054 forwardTM N out
2
148 LI;001527.1;2000SEP08405 491 forwardTM N out
3
148 LI:001527.1:2000SEP08705 791 forwardTM N out
3
148 LI:001527.1;2000SEP08936 986 forwardTM N out
3
148 LI;001527.1:2000SEP08984 1070 forwardTM N out
3
149 LI:395063.1:2000SEP08331 387 forwardTM N in
1
149 LI;395063.1:2000SEP08517 603 forwardTM N in
1
149 LI:395063.1:2000SEP08748 834 forwardTM N in
1
149 LI:395063.1;2000SEP08850 912 forwardTM N in
1
149 LI:395063.1;2000SEP08982 1068 forwardTM N in
1
149 LI:3950b3.1;2000SEP0811111197 forwardTM N in
1
149 LI;395063.1:2000SEP0815281614 forwardTM N in
1
149 LI:395063.1;2000SEP081,6151677 forwardTM N in
1
149 LI:395063.1:2000SEP0827762862 forwardTM N in
1
149 LI;395063.1:2000SEP08338 424 forwardTM N out
2
149 LI:395063.1:2000SEP08464 526 forwardTM N out
2
149 LI:395063.1:2000SEP08557 619 forwardTM N out
2
149 LI:3950b3.1:2000SEP08683 769 forwardTM N out
2
149 LI:395063.1:2000SEP08860 931 forwardTM N out
2
149 LI:395063.1:2000SEP0811211207 forwardTM N out
2
149 LI:395063.1;2000SEP0812591345 forwardTM N out
2
149 LI:395063.1:2000SEP0818261888 forwardTM N out
2
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template iD Start Frame Domain Topology
NO: Stop Type
149 LI:3950b3.1:2000SEP0822642314forward TM N out
2
149 LI:3950b3.1:2000SEP0824292515forward TM N out
2
149 LI:3950b3.1:2000SEP0811221199forward TM N in
3
149 LI:3950b3,1:2000SEP0816411727forward TM N in
3
149 LI:3950b3,1:2000SEP0824272513forward TM N in
3
149 LI:395063.1:2000SEP0828172867forward TM N in
3
150 LI:lOb4460.1:2000SEP08376 444 forward TM N out
1
150 LI:1064460.1:2000SEP08487 549 forward TM N out
1
150 LI:1064460.1:2000SEP08574 636 forward TM N out
1
150 LI:1064460.1:2000SEP08116 190 forward TM N out
2
150 LI:1064460.1:2000SEP08362 424 forward TM N out
2
150 LI:1064460.1:2000SEP08437 499 forward TM N out
2
150 LI: l Ob4460,1:2000SEP0854 116 forward TM N in
3
150 LI:1064460.1:20DOSEP08132 194 forward TM N in
3
150 LI:1064460.1:2000SEP08210 272 forward TM N in
3
150 LI:1064460.1:2000SEP08297 365 forward TM N in
3
150 LI:1064460.1:2000SEP08441 527 forward TM N in
3
150 LI:1064460.1:2000SEP08618 704 forward TM N in
3
150 LI:I 064460.1:2000SEP08750 836 forward TM N in
3
151 LI:344690,2:2000SEP08277 348 forward TM N out
1
151 LI:344b90,2:2000SEP08131 199 forward TM N in
2
151 LI:344690.2:2000SEP08284 337 forward TM N in
2
151 LI:344b90,2:2000SEP08129 215 forward TM N in
3
151 LI:344b90.2:2000SEP08465 524 forward TM N in
3
152 LI:061585,4:2000SEP08349 417 forward TM N out
1
152 LI:061585.4:2000SEP08628 714 forward TM N out
1
152 LI:061585,4:2000SEP08404 490 forward TM N in
2
152 LI:Ob1585,4:2000SEP08623 709 forward TM N in
2
152 LI:061585.4:2000SEP08953 1033forward TM N in
2
152 LI:061585.4:2000SEP08609 668 forward TM N in
3
152 LI:061585,4:2000SEP08882 962 forward TM N in
3
153 LI:378428.1:2000SEP0819572043forward TM N in
1
153 LI:378428,1:2000SEP0822332310forward TM N in
1
153 LI:378428.1:2000SEP08704 790 forward TM N in
2
153 LI:378428.1:2000SEP0822672353forward TM N in
2
153 LI:378428.1:2000SEP0813591445forward TM N out
3
153 LI:378428.1:2000SEP0816501715forward TM N out
3
153 LI:378428,1:2000SEP0819652027forward TM N out
3
154 LI:474108,2:2000SEP08982 1068forward TM N out
1
154 LI:474108,2:2000SEP0811321215forward TM N out
1
154 LI:474108.2:2000SEP0829022964forward TM N out
1
154 LI:474108,2:2000SEP0829923054forward TM N out
1
154 LI:474108.2:2000SEP0832593321forward TM N out
1
154 LI:474108,2:2000SEP0833943480forward TM N out
1
154 LI:474108.2;2000SEP0835803654forward TM N out
1
154 LI:474108,2:2000SEP0838593918forward TM N out
1
154 LI:474108.2:2000SEP0839464032foi~nrardTM N out
1
154 LI:474108.2:2000SEP0844504536forward TM N out
1
93
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID StartStop Frame Domain TypeTopology
NO;
154 LI:474108.2:2000SEP0846214707 forwardTM N out
1
154 LI:474108.2:2000SEP0847684854 forwardTM N out
1
154 LI;474108.2:2000SEP0811421219 forwardTM N out
2
154 LI:474108.2:2000SEP0812801360 forwardTM N out
2
154 LI:474108.2:2000SEP0815081570 forwardTM N out
2
154 LI:474108.2;2000SEP0815801666 forwardTM N out
2
154 LI;474108.2:2000SEP0818411909 forwardTM N out
2
154 LI;474108.2:2000SEP0820452101 forwardTM N out
2
154 LI:474108.2:2000SEP0824232506 forwardTM N out
2
154 LI;474108.2:2000SEP0826482734 forwardTM N out
2
154 LI:474108.2;2000SEP0829062992 forwardTM N out
2
154 LI:474108.2:2000SEP0833743460 forwardTM N out
2
154 LI:474108.2;2000SEP0835033565 forwardTM N out
2
154 LI:474108.2:2000SEP0835783640 forwardTM N out
2
154 LI;474108.2;2000SEP0838033889 forwardTM N out
2
154 LI;474108.2:2000SEP0843194372 forwardTM N out
2
154 LI:474108.2:2000SEP0845684654 forwardTM N out
2
154 LI;474108.2;2000SEP0851175179 forwardTM N out
2
154 LI:474108.2:2000SEP0830 116 forward~ TM N out
3
154 LI:474108.2:2000SEP0814191478 forwardTM N out
3
154 LI:474108.2;2000SEP0816021664 forwardTM N out
3
154 LI;474108.2:2000SEP0816801742 forwardTM N out
3
154 LI:474108.2;2000SEP0820882174 forwardTM N out
3
154 LI:474108.2:2000SEP0829012987 forwardTM N out
3
154 LI:474108.2;2000SEP0830933179 forwardTM N out
3
i54 LI:474108.2:2000SEP0834i 3497 forwardTM N out
1 3
154 LI;474108.2:2000SEP0835613647 forwardTM N out
3
154 LI:474108.2;2000SEP0842694325 forwardTM N out
3
154 LI;474108.2:2000SEP0845844637 forwardTM N out
3
154 LI:474108.2:2000SEP0847584844 forwardTM N out
3
154 LI;474108.2:2000SEP0852055276 forwardTM N out
3
155 LI:230711.2:2000SEP0872T 795 forwardTM N in
1
155 LI:230711.2;2000SEP08880 963 forwardTM N in
1
155 LI;230711.2:2000SEP0816961767 forwardTM N in
1
155 LI;230711.2:2000SEP08863 949 forwardTM
2
155 LI:230711.2:2000SEP0810851168 forwardTM
2
155 LI;230711.2:2000SEP08147 233 forwardTM N out
3
155 LI:230711.2:2000SEP08720 803 forwardTM N out
3
155 LI:230711.2:2000SEP0810801142 forwardTM N out
3
155 LI:230711.2:2000SEP0811551217 forwardTM N out
3
155 LI;230711.2;2000SEP0816201706 forwardTM N out
3
155 LI:230711.2:2000SEP0818151874 forwardTM N out
3
156 LI;008942.1:2000SEP08253 324 forwardTM N out
1
156 LI:008942.1;2000SEP0813091371 forwardTM N out
1
156 LI:008942.1:2000SEP0813931455 forwardTM N out
1
156 LI;008942.1;2000SEP08359 445 forwardTM N out
2
156 LI;008942.1:2000SEP08797 871 forwardTM N out
2
156 LI:008942.1:2000SEP0813551441 forwardTM N out
_ 2
_ .___
94
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID StartStop Frame Domain TypeTopology
NO:
156 LI:008942,1:2000SEP08696 776 forwardTM N in
3
156 LI:008942,1:2000SEP0813711442 forwardTM N in
3
156 LI:008942,1:2000SEP0819141997 forwardTM N in
3
157 LI:732479.1:2000SEP08649 723 forwardTM N out
1
157 LI:732479.1:2000SEP08721 807 forwardTM N out
1
157 LI:732479.1:2000SEP08829 909 forwardTM N out
1
157 LI:732479,1:2000SEP081084i forwardTM N out
134 1
157 LI:732479.1:2000SEP0812611323 forwardTM N out
1
157 LI:732479.1:2000SEP0814231509 forwardTM N out
1
157 LI:732479.1:2000SEP08869 928 forwardTM N in
2
157 LI:732479.1:2000SEP0812201306 forwardTM N in
2
157 LI:732479.1:2000SEP08534 605 forwardTM N in
3
157 LI:732479.1:2000SEP08846 902 forwardTM N in
3
157 LI:732479.1:2000SEP0815061568 forwardTM N in
3
158 LI:1190250,1:2000SEP08570 629 forwardTM N out
3
159 LI:1013717.1:2000SEP0825 87 forwardTM N out
1
159 LI:1013717,1:2000SEP08115 177 forwardTM N out
1
i 59 LI: i 0 i 3717.1:2000SEP08220 297 forwardTM N out
1
159 LI:1013717,1:2000SEP08352 438 forwardTM N out
1
159 LI:1013717,1:2000SEP08511 582 forwardTM N out
1
159 LI:1013717,1:2000SEP08407 478 forwardTM N in
2
159 LI:1013717,1:2000SEP08617 673 forwardTM N in
2
159 LI:1013717,1:2000SEP08812 868 forwardTM N in
2
159 LI:1013717.1:2000SEP08114 200 forwardTM N out
3
159 LI:1013717,1:2000SEP08309 392 forwardTM N out
3
1 b0 LI:2049125.2:2000SEP08129 215 forwardTM
3
161 LI:1092360,1:2000SEP0843 114 forwardTM N out
1
161 LI:1092360,1:2000SEP08400 474 forwardTM N out
1
lbl LI:1092360.1:2000SEP0865 118 forwardTM N out
2
161 LI:1092360.1:2000SEP08444 530 forwardTM N out
3
162 LI:791524,1:2000SEP0810 75 forwardTM N out
1
162 LI:791524.1:2000SEP08115 189 forwardTM N out
1
162 LI:791524.1:2000SEP0826 88 forwardTM N out
2
162 LI:79i 524,1:2000SEP08122 184 forwardTM N out
2
162 LI:791524,1:2000SEP0827 92 forwardTM N in
3
163 LI:1084555.3:2000SEP0837 123 forwardTM N in
1
1 b3 LI:1084555.3:2000SEP08176 256 forwardTM N in
2
163 LI:1084555,3:2000SEP08332 415 forwardTM N in
2
163 LI:1084555,3:2000SEP08' 158 forwardTM N out
72 3
1 b4 LI:8,15418,2:2000SEP0819852071 forwardTM N out
2
1 b4 LI:815418.2:2000SEP0819532039 forwardTM N in
3
165 LI:41 6766,1:2000SEP08259 318 forwardTM N in
1
1 b5 LI:416766.1:2000SEP08343 405 forwardTM N in
1
165 LI:416766.1:2000SEP08418 480 forwardTM N in
1
165 LI:416766.1:2000SEP0813121398 forwardTM N in
1
165 LI:416766.1:2000SEP0829563030 forwardTM N in
1
165 LI:416766,1:2000SEP0830973183 forwardTM N in
1
165 LI:416766.1:2000SEP0832773333 forwardTM N in
___- 1
9j
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID StartStop Frame Domain Topology
NO: Type
165 LI:416766,1;2000SEP08626 694 forwardTM N in
2
165 LI:416766.1:2000SEP08989 1051 forwardTM N in
2
165 LI:41b76b.1;2000SEP0812861372 forwardTM N in
2
1 b5 LI:4167bb.1;2000SEP0829723034 forwardTM N in
2
165 LI;416766,1:2000SEP0830623124 forwardTM N in
2
1 b5 LI:416766.1:2000SEP08375 455 forwardTM N in
3
165 LI;416766.1:2000SEP0812901376 forwardTM N in
3
1 b5 LI;41 6766,1:2000SEP0829403026 forwardTM N in
3
165 LI:416766.1;2000SEP0832343305 forwardTM N in
3
166 LI;1171008,2:2000SEP0861 117 forwardTM N in
1
1 b7 LI:11 b9888.3;2000SEP0879 165 forwardTM
1
1 b7 LI:1169888.3:2000SEP08286 372 forwardTM
1
i67 LI:1169888,3;2000SEP08505 591 forwardTM
i
1 b7 LI:1169888.3:2000SEP08781 864 forwardTM
1
1 b7 LI:1169888.3:2000SEP08913 999 forwardTM
i
167 LI:11b9888.3;2000SEP0898 178 forwardTM N in
2
1 b7 LI:1169888.3;2000SEP08290 352 forwardTM N in
2
1b7 LI:11b9888,3:2000SEP08374 436 forwardTM N in
2
167 LI;1169888.3:2000SEP08518 604 forwardTM N in
2
167 LI:11 b9888.3:2000SEP08704 754 forwardTM N in
2
167 LI;1169888.3:2000SEP08758 832 forwardTM N in
2
167 LI;1169888.3:2000SEP08866 952 forwardTM N in
2
1 b7 LI:1169888,3;2000SEP0893 155 forwardTM N out
3
1 b7 LI:11 b9888,3:2000SEP08186 248 forwardTM N out
3
1 b7 LI;1169888.3:2000SEP08402 473 forwardTM N out
3
1 b7 LI:1169888.3;2000SEP08519 581 forwardTM N out
3
1 b7 LI;1169888.3:2000SEP08591 653 forwardTM N out
3
1 b7 LI:1169888.3;2000SEP08813 893 forwardTM N out
3
1 b7 LI;11 b9888.3:2000SEP08963 1013 forwardTM N out
3
1 b8 LI;412592,1:2000SEP08145 225 forwardTM
1
1 b8 LI;412592,1;2000SEP0811381224 forwardTM
1
T 69 LI:349808.1:2000SEP0822 108 forwardTM N out
1
1 b9 LI:349808,1;2000SEP0825482613 forwardTM N out
1
169 LI:349808,1:2000SEP0826742730 forwardTM N out
T
1 b9 LI;349808,1;2000SEP0821382224 forwardTM N in
2
169 LI:349808,1:2000SEP0819021961 forwardTM N in
3
1 b9 LI:349808,1:2000SEP0827122765 forwardTM N in
3
170 LI:3491 b4,2:2000SEP08139 21 forwardTM N out
b 1
170 LI:349164.2:2000SEP082i4 300 forwardTM N out
1
170 LI:3491 b4.2;2000SEP08325 411 forwardTM N out
1
170 LI:349164.2:2000SEP08442 489 forwardTM N out
1
170 LI:349164,2;2000SEP08125 211 forwardTM N out
2
170 LI:349164.2:2000SEP08344 430 forwardTM N out
2
170 LI:349164,2:2000SEP08515 601 forwardTM N out
2 ~
170 LI;349164.2:2000SEP08998 1057 forwardTM N out
2
170 LI:3491 b4.2;2000SEP0812 68 forwardTM N out
3
170 LI:349164.2:2000SEP0896 182 forwardTM N out
3
170 LI:349164.2:2000SEP08315 401 forwardTM N out
3
96
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain TypeTopology
NO: Stop
170 LI:349164.2:2000SEP08543 611forward TM N out
3
171 LI;20541 3.1;2000SEP082194 2280forward TM N in
1
171 LI:205413.1:2000SEP082171 2233forward TM N in
2
171 LI;205413.1:2000SEP082600 2680forward TM N in
2
172 LI;2051508.2;2000SEP08268 354forward TM N out
1
173 LI;34b242.2:2000SEP08983 1042forward TM N out
2
173 LI;346242.2:2000SEP08882 9b8forward TM N in
3
173 LI;34b242.2;2000SEP081488 1574forward TM N in
3
174 LI;2052717.1:2000SEP081396 1470forward TM N in
1
174 LI:2052717.1;2000SEP081513 1575forward TM N in
1
174 LI:2052717.1:2000SEP081609 1671forward TM N in
1
174 LI;2052717.1:2000SEP081463 1534forward TM N out
2
174 LI:2052717.1:2000SEP081532 1615forward TM N out
2
174 LI;2052717.1:2000SEP081635 1700forward TM N out
3
175 LI;4066b8.2:2000SEP081474 1545forward TM N in
1
1 75 Li:406668.2:2000SEP081888 1 forward TM N in
9441
175 LI:406668.2;2000SEP082191 2277forward TM N in
1
175 LI;406668.2:2000SEP081871 1957forward TM N in
2
175 LI:40bb68.2;2000SEP082228 2290forward TM N in
2
175 LI;406668.2:2000SEP082109 2192forward TM N in
3
1 76 LI:1178352.1;2000SEP08568 654forward TM N in
1
176 LI:1178352.1;2000SEP081279 1365forward TM N in
1
176 LI:1178352.1:2000SEP081654'1716forward TM N in
1
176 LI;1178352.1:2000SEP081741 1803forward TM N in
1
176 LI:1178352.1;2000SEP082077 2148forward TM N in
1
17b LI;1178352.1:2000SEP0868 154forward TM N out
2
176 LI;1178352.1;2000SEP08359 442forward TM N out
2
176 LI:1178352.1:2000SEP081643 1729forward TM N out
2
176 LI:I 178352.1;2000SEP0821 107forward TM N out
3
176 LI:1178352.1:2000SEP08534 596forward TM N out
3
176 LI:I 178352.1:2000SEP08627 689forward TM N out
3
1 76 LI:1178352.1;2000SEP08705 782forward TM N out
3
176 LI:1178352.1;2000SEP08852 905forward TM N out
3
177 LI;814014.7:2000SEP08607 b69forward TM N out
1
177 LI:814014.7:2000SEP08599 685forward TM N out
2
177 L1:814014.7:2000SEP081 1193forward TM N out
107 3
178 LI:I 170624.1:2000SEP08299 358forward TM N out
2
178 LI:1 1 70624.1;2000SEP08443 520forward TM N out
2
179 LI;1183171.1:2000SEP08166 237forward TM
1
179 LI:1183171.1:2000SEP0829 115forward TM N out
2
179 L1:1183171.1;2000SEP081 218forward TM N out
b8 3
180 LI:1093491.1;2000SEP08154 207forward TM N in
1
180 LI:1093491.1;2000SEP08562 648forward TM N in
1
180 LI; 1 093491.1:2000SEP08649 702forward TM N in
1
180 LI:1093491.1;2000SEP0873b 807forward TM N in
1
180 LI;1093491.1:2000SEP08575 655forward TM N in
2
180 LI;1093491.1;2000SEP08695 748forward TM N in
2
180 LI:1093491.1:2000SEP08618 _689forward TM N in
~ 3
97
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 2
SEQ ID Template ID Start Frame Domain Topology
NO. Stop Type
181 LI;04b515.5;2000SEP084b0 546 forward TM N in
1
181 LI:046515.5:2000SEP0817 82 forward TM N out
2
181 LI;046515.5:2000SEP08314 388 forward TM N out
2
181 LI:04b5T5.5:2000SEP08515 571 forward TM N out
2
181 LI:04b515.5:2000SEP08141 209 forward TM N out
3
181 LI;046515.5;2000SEP08351 419 forward TM N out
3
181 LI:04b515.5:2000SEP08447 533 forward TM N out
3
T 82 LI:400T 71.2:2000SEP08511 597 forward TM N out
T
182 LI:400171.2:2000SEP08567 653 forward TM N out
3
183 LI:330919.6:2000SEP0870 150 forward TM N out
1
183 LI:330919.b;2000SEP08151 219 forward TM N out
1
183 LI;330919.b:2000SEP08256 309 forward TM N out
1
183 LI;330919.b;2000SEP08313 399 forward TM N out
1
183 LI;330919.b:2000SEP0856 130 forward TM N out
2
183 LI:330919.b:2000SEP08128 208 forward TM N out
2
183 LI:330919.6;2000SEP08311 358 forward TM N out
2
183 LI;330919.b;2000SEP0863 113 forward TM N in
3
183 LI:330919.b:2000SEP08135 194 forward TM N in
3
183 LI:330919.b:2000SEP08297 353 forward TM N in
3
184 LI:219502.1;2000SEP08316 402 forward TM N out
1
184 LI;219502.1:2000SEP08487 540 forward TM N out
1
184 LI:219502.1:2000SEP08233 319 forward TM N in
2
184 LI:219502.1:2000SEP08324 410 forward TM
3
184 LI:219502.1;2000SEP08861 923 forward TM
3
98
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
1 LG:983076,3:2000SEP087763237)1 782 1359
1 LG:983076.3:2000SEP087763237H1 527 1177
1 LG:983076,3:2000SEP086816661 J 303 880
1
1 LG:983076.3:2000SEP086816661 H 1 424
1
2 LG:1382987,7:2000SEP087221401H1 71 612
2 LG:1382987.7:2000SEP085539617H2 697 902
2 LG:1382987.7:2000SEP086911289) 188 790
1
2 LG:1382987,7:2000SEP087039246H1 1 489
3 LG:235557.15:2000SEP087013257H1 1 267
3 LG:235557,15:2000SEP083190036R6 1 267
3 LG:235557.15:2000SEP083190036H1 1 164
3 LG:235557.15:2000SEP083974159H1 140 380
3 LG:235557,15:2000SEP087714837) 197 291
1
3 LG:235557.15:2000SEP087714836H1 197 261
4 LG:O 7 8494.1:2000SEP08g5878576 358 801
4 LG:018494.1:2000SEP082875821 H1 665 800
4 LG:018494,1:2000SEP082875821 Fb 664 800
4 LG:018494.1:2000SEP08144378076 315 761
4 LG:018494.1:2000SEP08433194779 106 698
4 LG:O i 8494. i 433192079 150 671
:2000SEP08
4 LG:018494.1:2000SEP087267178H2 103 605
4 LG:018494.1:2000SEP08g6397885 133 294
4 LG:018494.1:2000SEP084676232H1 1 264
4 LG:018494.1:2000SEP082868107H1 665 805
LG:980494.1:2000SEP08g5541169 1219 1543
5 LG:980494.1:2000SEP08g2958236 1226 1414
5 LG:980494,1:2000SEP081 6l 875676 1247 1841
5 LG:980494,1:2000SEP087618177) 1629 21 O1
1
5 LG:980494,1:2000SEP081943565H1 1698 1877
5 LG:980494.1:2000SEP08g3331178 1075 1412
5 LG:980494,1:2000SEP086713531 H 11 b9 1537
1
5 LG:980494.1:2000SEP087683622H1 949 1454
5 LG:980494,1:2000SEP087682580H1 949 1455
5 LG:980494,1:2000SEP081784942H 957 1189
1
5 LG:980494.1:2000SEP08g6451273 1050 1412
5 LG:980494,1:2000SEP087684929H 949 1476
1
5 LG:980494.1:2000SEP08g3092119 930 1415
5 LG:980494,1:2000SEP08g2726574 947 11 b7
5 LG:980494,1:2000SEP084291592H 790 942
1
5 LG:980494,1:2000SEP084145168H 829 1119
1
5 LG:980494.1:2000SEP084125405H1 872 1148
5 LG:980494.1:2000SEP082078150H 742 1002
1
5 LG:980494,1:2000SEP08g 1785426 748 1188
5 LG:980494,1:2000SEP082430567H1 755 1007
5 LG:980494.1:2000SEP084576269F6 770 1374
5 LG:980494.1:2000SEP084291692H 788 881
1
5 LG:980494,1:2000SEP082214403H1 627 874
5 LG:980494.1:2000SEP084713559H1 499 750
~_._
99
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
LG:980494.1;2000SEP082214403F6 627 1059
5 LG:980494.1:2000SEP082212765H 1 627 881
5 LG:980494.1:2000SEP085515783H1 703 959
5 LG:980494.1;2000SEP084290144H1 738 996
5 LG:980494.1;2000SEP086725205H1 1 614
5 LG:980494.1;2000SEP08596464379 122 634
5 LG:980494.1:2000SEP08g2783200 342 848
5 LG;980494.1:2000SEP081618504H1 447 671
5 LG;980494.1;2000SEP081618756F6 447 854
5 LG:980494.1;2000SEP081618749H 1 447 649
5 LG:980494.1;2000SEP081686804H 1 450 670
5 LG:980494.1;2000SEP081785019H1 450 698
5 LG;980494.1:2000SEP08g2835145 474 737
6 LG:984457.2:2000SEP086784190H1 435 952
b LG:984457.2:2000SEP086715771 H 454 976
1
b LG:984457.2:2000SEP083368817F6 534 1083
6 LG:984457.2;2000SEP083368817H1 534 807
b LG:984457.2;2000SEP087353082H1 560 1130
b LG:984457.2;2000SEP085754149H1 750 1273
b LG;984457.2:2000SEP08249056976 1 546
6 LG:984457.2;2000SEP08379946776 25 358
6 LG:984457.2;2000SEP083413777H1 321 565
6 LG;984457.2;2000SEP087674559J1 396 946
7 LG:406758.1:2000SEP08g711128 930 1174
7 LG:406758.1:2000SEP082270260H1 803 1065
7 LG:406758.1;2000SEP083203437F6 518 1024
7 LG:406758.1;2000SEP085768790H 1 439 1013
7 LG;40b758.1;2000SEP082573541 H1 555 809
7 LG:406758.1;2000SEP083203437H1 520 770
7 LG:40b758.1;2000SEP086777565H1 1 634
7 LG;406758.1:2000SEP087690831 J 229 618
1
7 LG:406758.1:2000SEP085047721 R6 37 350
7 LG:406758.1;2000SEP087338496H1 867 1358
7 LG:406758.1;2000SEP083249541 H 1040 1354
1
7 LG;406758.1;2000SEP085690615H1 1042 1310
7 LG;40b758.1:2000SEP08g696761 930 1277
7 LG:40b758.1;2000SEP08g734852 930 1228
7 LG;406758.1;2000SEP08409935579 1518 1663
7 LG;40b758.1;2000SEP08g718527 1446 1656
7 LG;406758.1;2000SEP08g734770 1392 1647
7 LG:406758.1:2000SEP08g696594 1443 1647
7 LG:40b758.1;2000SEP08320343776 1124 1645
7 LG:40b758.1;2000SEP08424320876 1340 1645
7 LG:406758.1;2000SEP08g4451158 1390 1645
7 LG:406758.1:2000SEP08g4564394 1365 1645
7 LG;40b758.1:2000SEP08g3679317 1324 1645
7 LG;40b758.1;2000SEP08g2838157 1304 1645
7 LG:40b758.1:2000SEP08133437876 1315 1645
100
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
7 LG;406758.1:2000SEP087747262H1 1271 1645
7 LG;406758.1;2000SEP083343574Th 1206 1645
7 LG:406758.1:2000SEP081334378Fb 1156 1517
7 LG;406758.1:2000SEP081334378H 1156 1410
1
8 LG:902957.17:2000SEP085742915H1 1 294
9 LG:333179.1;2000SEP08g703579 183 475
9 LG;333179.1:2000SEP08g713006 183 4b4
9 LG;333179.1;2000SEP086026950H1 1 275
9 LG:333179.1;2000SEP085800088H 1 625
1
9 LG;333179.1:2000SEP081746609H1 5 2b1
9 LG;333179.1:2000SEP081746609Fb 5 555
9 LG:333179.1:2000SEP082006872H 6 148
1
9 LG:333179.1;2000SEP082473167F6 b 4b9
9 LG:333179.1:2000SEP082473167H 6 239
1
9 LG;333179.1:2000SEP08033784H1 27 298
9 LG:333179.1:2000SEP085282372H2 27 207
9 LG:333179.1:2000SEP084997b89H 33 287
1
9 LG:333179.1:2000SEP08g2034987 39 323
9 LG:333179.1;2000SEP08030695H 1 39 241
9 LG:333179.1:2000SEP08g2154206 61 161
9 LG;333179.1:2000SEP084995956H1 79 340
9 LG;333179.1:2000SEP086246345H1 1 526
9 LG:333179.1;2000SEP08g1924106 1 369
9 LG;333179.1;2000SEP08171289H 1 1 230
9 LG:333179.1:2000SEP08007672H 1 195 4b3
9 LG:333179.1;2000SEP084400180H 203 308
1
9 LG;333179.1:2000SEP084198119H1 221 473
9 LG:333179.1;2000SEP08gb697567 328 786
9 LG;333179.1:2000SEP08g4735257 332 784
9 LG;333179.1;2000SEP08g6661884 338 782
9 LG;333179.1;2000SEP08g4739770 351 786
9 LG;333179.1;2000SEP0883400569 360 .792
9 LG:333179.1;2000SEP081975405H1 370 556
9 LG;333179.1:2000SEP0883144451 371 782
9 LG:333179.1;2000SEP0884125056 375 791
9 LG:333179.1:2000SEP0885804179 381 786
9 LG;333179.1;2000SEP0884741035 384 785
9 LG:333179.1:2000SEP088280b047 387 787
9 LG;333179.1:2000SEP08864627b9 387 785
9 LG;333179.1:2000SEP0885765850 414 790
9 LG:333179.1:2000SEP0882821034 422 787
9 LG:333179.1;2000SEP0885631640 453 783
9 LG;333179.1;2000SEP0883737203 454 608
9 LG:333179.1:2000SEP0885393676 478 785
9 LG;333179.1:2000SEP0883118453 485 785
9 LG:333179.1;2000SEP0883162475 487 785
9 LG:333179.1:2000SEP08gb5b7793 503 787
9 LG;333179.1;2000SEP082010119H1 534 642
101
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
9 LG;333179.1;2000SEP082473167Th 613 739
9 LG:333179.1:2000SEP08g703479 648 933
9 LG:333179.1:2000SEP08g723722 674 921
LG:406568.1;2000SEP082324266H1 1336 1578
10 LG;406568.1;2000SEP083011865F6 1339 1760
10 LG:406568.1:2000SEP083576519H1 1338 1494
10 LG:406568. i ;2000SEP085176829H 1347 1599
l
10 LG:4065b8.1;2000SEP083873606H1 1350 1660
10 LG:406568.1;2000SEP084852614H 1362 1586
1
10 LG:406568.1;2000SEP087753572) 1376 1936
1
10 LG:406568. i :2000SEP083958442H 1376 1473
i
10 LG:406568.1:2000SEP083016113F6 1375 1646
10 LG;406568.1;2000SEP08058529H1 1375 1521
10 LG:406568.1;2000SEP085276870H1 1375 1534
10 LG:406568.1:2000SEP083011865H 1375 1458
1
10 LG:406568.1:2000SEP083578346H1 1381 1633
10 LG:406568.1:2000SEP083016113H 1382 1668
1
10 LG;406568.1;2000SEP086337413H 1389 1506
1
10 LG:406568.1:2000SEP086338013H1 1389 1880
10 LG;406568.1:2000SEP086335720H 1389 l 882
1
10 LG:406568.1:2000SEP083688195H1 1397 1694
10 LG;406568.1;2000SEP084151882H 1400 1640
1
10 LG:4065b8.1:2000SEP083874704Hi 1438 1713
10 LG;406568.1;2000SEP085169119H 1475 1609
1
10 LG:406568.1:2000SEP085278359H1 1474 1704
10 LG:40b568.1:2000SEP083685458H1 1485 1782
10 LG;40b568.1:2000SEP086332978H i 486 198
1 i
10 LG:406568.1:2000SEP085531678H 1494 1623
1
10 LG;40b5b8.1:2000SEP08g5395484 1512 1853
10 LG:406568.1;2000SEP083693626H 1511 1803
1
10 LG;4065b8.1:2000SEP084152946H1 1519 1791
10 LG:40b568.1;2000SEP083890704H 1547 1841
1
10 LG:40b568.1:2000SEP083875504H1 1548 1850
10 LG:406568.1:2000SEP084466716H1 1581 1838
10 LG:40b568.1:2000SEP08g395449 1590 1922
10 LG;40b5b8.1;2000SEP085i67337H1 1591 1799
10 LG:406568.1:2000SEP08g6836605 1592 1891
10 LG;4065b8.1:2000SEP08920392H 1 1610 1922
10 LG:406568.1;2(~OOSEP083011865Th 1617 ~ 1971
10 LG:406568. i :2000SEP08301 b i 13Th1673 2154
10 LG;406568.1:2000SEP085277911 H1 1696 1934
10 LG:406568.1:2000SEP083874759H 1 295
1
10 LG;406568.1;2000SEP083685395H1 3 270
10 LG:406568.1:2000SEP08g311 6688 4 442
10 LG:406568.1;2000SEP083692640H1 4 269
10 LG:40b568.1:2000SEP083692640F6 4 341
10 LG:406568.1:2000SEP083445829H2 6 263
10 LG:406568.1:2000SEP08306745H 1 13 385
102
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
LG:40b568,1:2000SEP08945423H 1 11 292
10 LG:4065b8.1:2000SEP087752844H1 21 658
10 LG:406568.1:2000SEP087369625H 40 556
1
10 LG:40b568,1:2000SEP0898824881 178 382
10 LG:406568.1:2000SEP08988248H1 178 290
10 LG:40b5b8.1:2000SEP084454887H1 249 518
10 LG:406568.1:2000SEP087753572H 272 808
1
10 LG:406568,1:2000SEP083877090H1 349 618
10 LG:40b568.1:2000SEP086901148H 367 824
1
10 LG:4065b8.1:2000SEP083028782F6 504 840
10 LG:406568.1:2000SEP083028782Hi 504 806
10 LG:406568.1:2000SEP087752949)1 532 1080
10 LG:4065b8,1:2000SEP087416011 Tl 548 952
10 LG:406568.1:2000SEP087754049H1 569 1211
10 LG:40b568.1:2000SEP081005592H1 598 888
10 LG:406568.1:2000SEP087754049) 639 1265
1
10 LG:406568.1:2000SEP087752949H1 683 1175
10 LG:40b5b8, l :2000SEP087753382) 726 1 i
1 18
10 LG:406568.1:2000SEP087752844)1 800 1346
10 LG:40b568.1:2000SEP084646393H1 800 1060
10 LG:40b568,1:2000SEP085278286H1 911 1124
10 LG:40b568.1:2000SEP082521969F6 949 1353
10 LG:40b568.1:2000SEP08983403H 1 1062 1334
10 LG:406568.1:2000SEP0898340386 1063 1314
10 LG:406568.1:2000SEP08188949H 1 1105 1305
10 LG:406568.1:2000SEP08983403T6 1122 1346
10 LG:406568.1:2000SEP08988925H 1 1125 1348
10 LG:40b568.1:2000SEP082521969H1 1128 1353
10 LG:406568. i :2000SEP082639257H 1176 1338
1
10 LG:406568.1:2000SEP08983273H1 1240 1543
10 LG:406568.1:2000SEP083692640T6 1246 1831
10 LG:406568.1:2000SEP084013213H1 1307 1597
10 LG:406568.1:2000SEP084010336H 1327 1610
1
10 LG:4065b8.1:2000SEP083877453H1 1330 1613
10 LG:406568,1:2000SEP083013979H 1876 21 b8
1
10 LG:406568.1:2000SEP08921802H1 1887 2213
10 LG:40b568.1:2000SEP08920885H1 1887 2203
10 LG:406568.1:2000SEP085278931 H1 1995 2213
10 LG:4065b8.1:2000SEP08058768H1 1997 2200
10 LG:40b568.1:2000SEP083045908H1 2012 2280
10 LG:406568,1:2000SEP08g2787073 2047 2206
10 LG:4065b8.1:2000SEP08979691 H1 1835 2119
10 LG:406568.1:2000SEP08g434193 1851 2025
7 0 LG:4065b8.1:2000SEP085803204H 1825 2023
1
10 LG:406568.1:2000SEP082636153H1 1699 1928
10 LG:406568.1:2000SEP081567405H 1705 1888
1
10 LG:406568.1:2000SEP0846251386 1705 2043
10 LG:406568,1:2000SEP08462513H1 1705 1930
.
~0J
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6~ Template ID Component Start Stop
ID NO: ID
LG:4065b8.1:2000SEP083877471 H1 1720 1989
10 LG;406568.1:2000SEP085277625H1 1722 1972
10 LG:40b568.1:2000SEP083016674H1 1738 2023
10 LG:406568.1:2000SEP083045188H1 1738 2024
10 LG:406568.1:2000SEP085299216H 1 1760 2000
10 LG;4065b8.1:2000SEP0897760571 1763 2178
10 LG:40b568.1:2000SEP08977605R1 1763 2142
10 LG:406568.1:2000SEP08977605H1 1763 2074
10 LG;4065b8.1:2000SEP083487465H1 1764 2053
10 LG:40b568.1:2000SEP08302878276 1766 2193
10 LG:4065b8.1:2000SEP08462261 H 1 1816 2013
10 LG:406568.1;2000SEP083683935H 1 1822 2108
10 LG:406568.1:2000SEP085170457H1 1822 2010
17 LG:353203.1:2000SEP08590602679 1 549
11 LG:353203.1:2000SEP085906026H1 7 299
11 LG;353203.1:2000SEP085906026F6 1 609
~
i 1 LG:353203.1:2000SEP085906026F8 7 277
11 LG:353203.1:2000SEP084569921 H 36 297
1
12 LG:061277.1:2000SEP081796939H 1 1 211
12 LG:061277.1:2000SEP081796939F6 1 504
12 LG:061277.1:2000SEP08g2934541 130 504
13 LG:170666.1:2000SEP083443916H1 88 361
13 LG:170666.1:2000SEP083443916F6 88 378
13 LG:170666.1;2000SEP08g4620823 258 606
13 LG:1706bb.1;2000SEP08g 1277971 1 191
13 LG;170666.1:2000SEP085834873F9 1 436
13 LG:170666.1:2000SEP085834873F8 1 470
13 LG:170666.1:2000SEP086327011 H 1 197
1
13 LG:170666.1;2000SEP086327111 H 1 282
1
13 LG:170b66.1;2000SEP086327111 F6 1 386
14 LG:311197.1;2000SEP086803514H1 503 984
14 LG:311197.1:2000SEP086803514) 1 242 823
14 ~ LG:311197.1:2000SEP086286909H2 316 799
14 LG:311197.1:2000SEP08g4568721 260 .685
14 LG:311 i 97.1:2000SEP08g3742689 209 684
14 LG:311197.1;2000SEP08g5365250 586 679
i 4 LG:311197, i :2000SEP08g5676607 451 679
14 LG:311197.1:2000SEP08g41 T 1591 263 679
14 LG:311197.1:2000SEP08g2539496 299 678
14 LG:3 i 1197,1;2000SEP08g5769156 224 677
14 LG:311197.1:2000SEP08g5232991 277 675
14 LG:311197.1:2000SEP08g3090059 202 676
14 LG:311197.1:2000SEP08g3751723 261 676
14 LG:311197.1:2000SEP08g4299176 248 676
14 LG;311197.1:2000SEP08g5630519 273 675
14 LG:311197.1:2000SEP08574367477 354 545
14 LG;311197.1;2000SEP085743674H1 1 297
14 LG;311197.1:2000SEP084127943H1 1 246
_ - __ _ ___
104
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ iD Template ID Component Start Stop
NO: ID
14 LG;311197.1;2000SEP08574367487 1 183
15 LG:220655.4:2000SEP086054260H1 1 534
15 LG:220655.4;2000SEP086053961 H 1 609
1
15 LG:220655.4:2000SEP087397741 H 417 i 004
1
16 LG:1001893.1:2000SEP086339672F8 1 624
16 LG:1001893.1:2000SEP086339672H1 1 590
1 b LG;1001893.1:2000SEP08633967278 483 868
17 LG:004335.1:2000SEP08824343H 1 193 T . 2213
17 LG;004335.1;2000SEP0882434386 1931 2448
17 LG:004335.1:2000SEP08261633776 1936 2505
17 LG;004335.1;2000SEP08745151771 1961 2473
17 LG:004335.1;2000SEP08589995476 1988 2499
17 LG:004335.1;2000SEP086866173H1 2014 2149
17 LG:004335.1:2000SEP086866273H 2014 2381
1
17 LG;004335.1;2000SEP08g1163587 1907 2156
17 LG:004335.1:2000SEP085895088H1 1910 2161
17 LG;004335.1:2000SEP08569738578 1925 2345
17 LG:004335.1;2000SEP085899954F6 1404 1958
17 LG:004335.1:2000SEP085897061H1 1404 1695
17 LG:004335.1:2000SEP085899922H 1404 1668
1
17 LG;004335.1:2000SEP084821288H1 1485 1757
17 LG;004335.1:2000SEP085602937H1 1497 1759
17 LG;004335.1:2000SEP08g4194776 1578 2040
17 LG:004335.1;2000SEP085697385F9 1627 1777
17 LG:004335.1:2000SEP087608724) i 738 230
1 i
17 LG;004335.1:2000SEP08g457371 b 1771 2190
17 LG:004335.1:2000SEP08g4573707 1771 2190
17 LG;004335.1;2000SEP086566503H1 1793 2347
17 LG:004335.1:2000SEP086211870H1 1797 2076
17 LG;004335.1;2000SEP087158924H1 1801 2071
17 LG;004335.1:2000SEP087037342H1 1807 1980
17 LG:004335.1:2000SEP08569738579 1889 2470
17 LG:004335.1;2000SEP086431671 H1 1907 2436
17 LG:004335.1:2000SEP083936275H1 6l 329
17 LG:004335.1:2000SEP086392874H1 99 369
17 LG;004335.1:2000SEP084726741H1 187 433
17 LG:004335.1:2000SEP084726741 F6 187 741
17 LG;004335.1:2000SEP08695i945H1 334 909
17 LG:004335.1;2000SEP085678094H1 477 738
17 LG:004335.1:2000SEP087082061H1 483 991
17 LG;004335.1:2000SEP086923277H1 512 1037
17 LG:004335.1:2000SEP084064256H1 708 944
17 LG:004335.1:2000SEP084064256F6 708 969
17 LG:004335.1:2000SEP087608724H 743 1204
1
17 LG:004335.1;2000SEP086426967H1 793 1368
17 LG;004335. i ;2000SEP086886093) 821 i 401
1
i 7 LG:004335.1:2000SEP082616337F6 846 1256
i7 LG:004335.1:200OSEP082616337H1 846 1099
10~
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
17 LG:004335.1:2000SEP087088351H1 927 1460
17 LG;004335.1;2000SEP086340018H1 931 1404
17 LG;004335.1;2000SEP084900977H1 976 1182
17 LG;004335.1:2000SEP084900977F9 991 1508
17 LG:004335.1:2000SEP086536713H 1 1019 1506
17 LG:004335.1:2000SEP085899231H1 1404 1686
17 LG:004335.1;2000SEP086872178H 1 1 274
17 LG;004335.1:2000SEP08g2784563 24 396
17 LG:004335.1;2000SEP086886093H 1 1 501
17 LG;004335.1:2000SEP08g2810592 1 176
17 LG:004335.1;2000SEP08g2896627 1 324
17 LG:004335.1:2000SEP08g3924468 ) 78
17 LG:004335.1;2000SEP08g4852361 1 260
17 LG;004335.1;2000SEP08g5233752 1 140
17 LG:004335.1;2000SEP082127851 H 2041 2307
1
17 LG;004335.1;2000SEP086436591 H 2054 2436
1
17 LG:004335.1:2000SEP08567809476 2073 2500
17 LG;004335.1:2000SEP08g5369879 2085 2545
17 LG;004335.1:2000SEP08g4684665 2087 2515
17 LG;004335.1;2000SEP08g3307962 2100 2544
17 LG;004335.1;2000SEP0882434376 2104 2511
17 LG:004335.1:2000SEP08g3841316 2116 2545
17 LG;004335.1;2000SEP087338633H1 2134 2538
17 LG:004335. i :2000SEP08g3162182 2 i 32 2545
17 LG:004335.1:2000SEP08g5530735 2142 2545
17 LG;004335.1;2000SEP08490097779 2175 2422
17 LG:004335.1:2000SEP08g6838724 2183 2545
17 LG:004335.1;2000SEP08g4983820 2188 2547
17 LG:004335.1;2000SEP08g4971326 2227 2543
17 LG:004335.1;2000SEP084726741 T6 2254 2512
17 LG;004335.1:2000SEP081799046H1 2259 2489
17 LG:004335.1:2000SEP08g5437793 2376 2546
17 LG:004335.1:2000SEP085878314H 1 2390 2544
18 LG:213092.b:2000SEP087394750H1 1 393
19 LG:407570.5;2000SEP082929935H1 1 288
19 LG:407570.5:2000SEP082929935F6 1 243
19 LG:407570.5;2000SEP083002522F6 19 306
19 LG:407570.5:2000SEP083002522H 1 20 329
19 LG:407570.5;2000SEP08195134976 137 634
19 LG:407570.5:2000SEP08165883876 148 531
19 LG;407570.5:2000SEP081658838F6 148 676
19 LG:407570.5;2000SEP081658838H1 148 376
20 LG:337835.8:2000SEP08g2079164 1 421
20 LG:337835.8;2000SEP084576482H1 1 119
20 LG:337835.8:2000SEP082817432H1 6 276
20 LG:337835.8:2000SEP085567451 H 10 227
1
20 LG:337835.8:2000SEP084631941 H1 10 271
20 LG;337835.8;2000SEP08_2444616F6 22 442
_ _ ___
lOG
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
20 LG:337835.8:2000SEP082444b16H1 22 ~ 245
20 LG:337835.8;2000SEP083535844H1 26 307
20 LG:337835.8:2000SEP0837370bOH1 27 193
20 LG:337835.8:2000SEP085293950H2 26 155
20 LG:337835.8;2000SEP086855696H1 50 654
20 LG:337835.8:2000SEP086955392H1 69 598
20 LG:337835.8:2000SEP08g 1984989 78 309
20 LG:337835.8;2000SEP084323489H1 111 339
20 LG:337835.8;2000SEP08517888H1 119 345
20 LG:337835.8;2000SEP0863177b4H1 160 443
20 LG;337835.8;2000SEP086317732H1 160 441
20 LG:337835.8;2000SEP086317796N 1 1 bb 443
20 LG:337835.8;2000SEP08g5231782 507 975
20 LG:337835.8;2000SEP08g56b4130 522 976
20 LG:337835.8:2000SEP081439187Fb 614 988
20 LG:337835.8;2000SEP081439187H1 614. 804
20 LG:337835.8:2000SEP08g46b43b5 700 9b9
20 LG:337835.8;2000SEP08323242H1 783 984
21 LG:1099283.1:2000SEP087415b54T2 1 470
21 LG:1099283.1;2000SEP08307b753H 1 4 2b2
21 LG:1099283.1:2000SEP084018474H1 16 233
21 LG;1099283.1;2000SEP084023162H 1 14 292
21 LG:1099283.1:2000SEP084023196H 1 15 291
21 LG:1099283.1;2000SEP083825381 H 120 401
1
21 LG:1099283.1:2000SEP084023549H T 133 442
21 LG:1099283.1:2000SEP085107901 H1 237 468
21 LG:1099283.1:2000SEP083073301 H 2b7 356
1
21 LG:1099283.1:2000SEP082346304F6 1 320
21 LG:1099283.1:2000SEP08591880H1 47 228
21 LG:1099283.1;2000SEP084017491 H 67 327
1
21 LG:1099283.1;2000SEP084020384H1 74 357
21 LG:1099283.1:2000SEP08593233H 1 47 223
21 LG:1099283.1:2000SEP085374682H 1 70 247
22 LG:401274,2:2000SEP087004550H1 1 548
22 LG:401274.2:2000SEP08gbb1242 10 108
23 LG;222880.1;2000SEP087081513H1 1 525
23 LG:222880.1:2000SEP087757 T 59N 188 788
1
23 LG:222880.1:2000SEP082509720H1 252 480
23 LG:222880.1:2000SEP0873b3062H1 332 912
23 LG:222880.1:2000SEP083242083H1 367 617
23 LG:222880.1;2000SEP083644b04H T 371 666
23 LG:222880.1;2000SEP083b44604Fb 3b9 627
23 LG;222880.1;2000SEP085741818H1 407 602
23 LG:222880.1:2000SEP087082116H1 438 981
23 LG:222880.1:2000SEP08957b45R6 1748 1887
23 LG:222880.1;2000SEP08957b45Tb 1748 1844
23 LG:222880.1:2000SEP08g2930525 1757 2092
23 LG:222880.1:2000SEP08_ _77b4378H1 582 1187
107
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
23 LG;222880.1:2000SEP082060770H1 626 870
23 LG;222880.1:2000SEP085113293H1 709 974
23 LG;222880, i ;2000SEP08g i 89 i 7 i 4 1 O
059 18
23 LG:222880.1:2000SEP086479360H 822 1303
1
23 LG:222880.1:2000SEP085784355H1 851 1113
23 LG:222880.1:2000SEP085794459H 851 1146
1
23 LG;222880.1:2000SEP085784455H 851 1086
1
23 LG:222880.1;2000SEP085784355F6 856 1391
23 LG:222880.1:2000SEP083734 i 22H 874 11 b0
1
23 LG;222880.1;2000SEP086832370H 874 1477
1
23 LG;222880.1;2000SEP086836219H 946 . 1257
1
23 LG;222880.1:2000SEP085104159H1 960 1199
.
23 LG;222880.1;2000SEP087757159)1 1009 1639
23 LG;222880.1;2000SEP084512707H1 1005 1260
23 LG;222880.1:2000SEP081836548H1 1055 1111
23 LG:222880.1:2000SEP081006724H 1061 1308
1
23 LG;222880.1;2000SEP08523339H 1 1125 1370
23 LG;222880.1:2000SEP08630148H1 1155 1437
23 LG;222880.1;2000SEP085897974H 1197 1466
1
23 LG;222880.1;2000SEP08459407H 1 1220 1503
23 LG:222880.1:2000SEP081930289F6 1290 1795
23 LG:222880.1;2000SEP081930289H1 1290 1560
23 LG;222880.1:2000SEP08193028976 1294 1818
23 LG;222880.1:2000SEP08578435576 1319 1729
23 LG:222880.1:2000SEP08365961876 1333 1821
23 LG:222880.1:2000SEP082762471 H 1354 1597
1
23 LG;222880.1:2000SEP083400826H1 1374 1601
23 LG;222880.1;2000SEP0883647867 1386 1793
23 LG:222880.1;2000SEP0884302533 1431 1885
.
23 LG:222880.1;2000SEP08954028H1 1437 1705
23 LG;222880.1;2000SEP0886398856 1471 1887
23 LG:222880.1;2000SEP0885368595 1475 1929
23 LG:222880.1;2000SEP0885662921 1485 1927
23 LG;222880.1;2000SEP0882279246 1492 _ 1887
23 LG;222880.1:2000SEP0883432804 1496 1929
23 LG:222880.1;2000SEP0883927268 1500 1897
23 LG;222880.1:2000SEP0883751389 1533 1886
23 LG:222880. i :2000SEP083028225H 1537 1818
i
23 LG:222880.1;2000SEP0883244749 1547 1892
23 LG:222880.1;2000SEP08169624176 1548 1868
23 LG:222880.1;2000SEP081696241 H 1555 1798
1
23 LG;222880.1:2000SEP081696241 F6 1555 1908
23 LG:222880.1;2000SEP0886471216 1558 1865
23 LG;222880.1:2000SEP0885110284 1566 2015
23 LG:222880.1:2000SEP0884373194 1573 1929
23 LG;222880. i ;2000SEP0885664143 1578 1927
23 LG:222880.1:2000SEP082293651 H 1577 1807
1
23 LG;222880.1;2000SEP0883 1586 1928
869595
_
_
108
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6~ Template ID Component Start Stop
ID NO: ID
23 LG;222880.1;2000SEP08725947H1 1586 1829
23 LG:222880.1:2000SEP08g24013b3 1590 1887
23 LG:222880.1:2000SEP086773275) 1 1593 2143
23 LG:222880.1;2000SEP08g 1156118 1607 1887
23 LG:222880.1:2000SEP087341844H 1 1648 1888
23 LG;222880.1;2000SEP08g1892265 1660 1926
23 LG;222880.1:2000SEP08g4242906 1678 18b1
23 LG:222880.1;2000SEP085399b80H 1 1685 1882
23 LG;222880.1:2000SEP08g7b4351 1721 1940
23 LG;222880.1;2000SEP08g2716497 1722 2099
23 LG;222880.1:2000SEP08g2804847 1744 2085
23 LG:222880.1;2000SEP08957645H 1 1748 1853
23 LG;222880.1:2000SEP0895764571 1748 1844
23 LG;222880.1:2000SEP08825718H1 1893 2188
23 LG:222880.1;2000SEP083169276Fb 1906 2316
23 LG;222880.1:2000SEP083169276H1 1907 ~ 2175
23 LG;222880.1:2000SEP084880539H1 2030 2263
23 LG;222880.1:2000SEP08551186H1 2089 2335
23 LG;222880.1:2000SEP085223525H1 2144 2295
23 LG:222880.1:2000SEP087734877H1 2168 2574
23 LG:222880.1:2000SEP08g 1959837 2259 2562
23 LG:222880.1:2000SEP08331447bF6 2281 2592
23 LG:222880.1:2000SEP083314476H1 2281 2531
23 LG:222880.1:2000SEP086773275H1 2282 2747
23 LG:222880.1;2000SEP08316927676 2501 2592
23 LG:222880.1:2000SEP082134443H 1 2501 2592
24 LG:406389.1:2000SEP08g42b4177 1 472
24 LG:406389.1;2000SEP086768921 J 1 570
1
24 LG:406389.1:2000SEP0867676b8) 1 1 462
24 LG:406389.1:2000SEP083787648H1 382 660
24 LG:406389.1;2000SEP08g709143 395 708
24 LG:40b389.1:2000SEP08g570324 4T 1 737
24 LG:40b389.1:2000SEP08gb94218 507 733
24 LG:406389.1:2000SEP083574983H1 642 930
24 LG:406389.1:2000SEP08g 1809833 642 903
24 LG:40b389.1:2000SEP084766304H 1 690 961
24 LG;406389.1;2000SEP084766304F6 690 1 Ob2
24 LG:406389.1:2000SEP08921701 H1 920 1242
24 LG;406389.1:2000SEP087091595H1 922 1457
24 LG:40b389.1:2000SEP0847b630476 1123 1508
24 LG;406389.1:2000SEP08g3308722 1139 1550
24 LG:406389.1:2000SEP086870105H1 1141 157b
24 LG:406389.1:2000SEP086870968H 1 1145 1 bbl
24 LG:40b389.1:2000SEP08g3805b40 1178 1554
24 LG:406389.1:2000SEP08g5b1297 1217 1550
24 LG;406389.1:2000SEP08g795551 1242 1560
24 LG:40b389. T :2000SEP088683370 12b6 1550
24 LG:40b389.1:2000SEP0882141803 1393 1554
109
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
24 LG;406389.1;2000SEP08478862776 1442 1509
25 LG:055461.1;2000SEP084592590H1 1 154
25 LG:0554b1.1:2000SEP084592590F8 1 566
25 LG:05546i.1:2000SEP082457736F6 14 468
25 LG:055461.1;2000SEP084194633H1 83 395
25 LG:0554b1.1:2000SEP084194633F7 87 672
25 LG:055461.1;2000SEP082073295H1 116 371
25 LG:055461.1;2000SEP082073295F6 116 472
25 LG;055461.1:2000SEP082895870H1 171 456
25 LG;055461.1;2000SEP08491179H1 219 482
25 LG;055461.1;2000SEP081400739H 280 523
1
25 LG;055461.1:2000SEP08228621 bRb 408 803
25 LG;0554b1.1:2000SEP082286216H1 408 591
25 LG;055461.1:2000SEP084670223H1 422 675
25 LG;055461.1;2000SEP087091663H1 710 1060
25 LG:055461.1:2000SEP084172005N 739 1009
1
25 LG:055461.1;2000SEP084172005F6 740 1250
25 LG;055461.1:2000SEP084054692H1 740 1019
25 LG:05546 i .1;2000SEP08240724076 i O 14 i 498
25 LG;055461.1;2000SEP08g6198775 1113 1514
25 LG:055461.1:2000SEP08g3118198 1149 1514
25 LG:0554b1.1:2000SEP081600869H1 1268 1516
25 LG;0554b1.1;2000SEP08g4329308 1292 1518
25 LG:055461.1:2000SEP08207329576 1409 1613
26 LG:979059.5:2000SEP087700332) 1 658
1
2b LG:979059.5:2000SEP081964843H 105 373
1
26 LG;979059.5:2000SEP081966204R6 112 354
26 LG:979059.5;2000SEP081966137H1 112 344
26 LG:979059.5;2000SEP081966137R6 112 570
26 LG:979059.5;2000SEP08g953428 112 , 303
26 LG:979059.5:2000SEP082542469H1 158 386
26 LG:979059.5:2000SEP08g766126 205 288
27 LG:399238.1:2000SEP08g4850653 1552 1974
27 LG:399238.1:2000SEP08g6475403 1800 1970
27 LG:399238.1:2000SEP08g3330309 1536 1967
27 LG;399238.1:2000SEP08g770433 1715 1963
27 LG:399238.1:2000SEP08g3888972 1717 1960
27 LG:399238.1:2000SEP08g4393908 1681 1961
27, LG:399238.1:2000SEP08g3843053 1681 1960
27 LG:399238.1;2000SEP081926452H 1899 1959
1
27 LG:399238.1:2000SEP08g3077521 1498 1959
27 LG:399238.1;2000SEP08g5877902 1486 1959
27 LG;399238.1:2000SEP08g3069392 1602 1956
27 LG:399238.1;2000SEP08g3109645 1544 1956
27 LG:399238.1:2000SEP08g2411261 1794 1956
27 LG:399238.1:2000SEP08g4083704 1511 1956
27 LG:399238.1:2000SEP08g3214844 1516 1956
27 LG:399238.1:2000SEP08g3077207 1505 1956
110
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE62 Template ID Component Start Stop
ID NO; ID
27 LG:399238.1;2000SEP08g4394965 1568 1954
27 LG:399238.1:2000SEP08g2715285 1646 1954
27 LG;399238,1:2000SEP08g5848683 1501 1953
27 LG;399238,1;2000SEP08g4153404 1608 1952
27 LG:399238.1;2000SEP08g4901426 1490 1951
27 LG;399238,1:2000SEP087336114H1 1453 1950
27 LG:399238,1;2000SEP08g4664620 1645 1950
27 LG:399238.1;2000SEP08g3888242 1533 1950
27 LG:399238.1:2000SEP08g4682808 1494 1950
27 LG:399238.1;2000SEP08g4111172 1531 1950
27 LG;399238,1:2000SEP08g5369024 1493 1950
27 LG;399238,1:2000SEP08g5589837 1492 1950
27 LG:399238.1:2000SEP08g4394945 1492 1950
27 LG:399238.1:2000SEP08g4148390 i 53 T 1950
27 LG;399238.1:2000SEP08g3174652 1473 1949
27 LG:399238,1;2000SEP08g3095707 1756 1941
27 LG:399238.1:2000SEP083930103T6 1409 1924
27 LG;399238.1:2000SEP08g2992787 1636 1914
27 LG;399238, 7 :2000SEP082102303T6 1318 1912
27 LG:399238.1:2000SEP082673478T6 1598 1912
27 LG:399238.1:2000SEP0828 T 60 T 1574 1866
2H 1
27 LG;399238.1:2000SEP08g4311150 1479 1826
27 LG:399238.1;2000SEP087102476H1 1528 ~ 1776
27 LG:399238.1:2000SEP08g201 6852 1442 1727
27 LG;399238.1;2000SEP083431253H1 1392 1641
27 ~ LG:399238.1;2000SEP083554944H 1 1352 1633
27 LG:399238,1:2000SEP083222446H 1 1282 1579
27 LG:399238.1:2000SEP08210230386 T 206 T 53
T
27 LG:399238.1:2000SEP082100380H1 1238 1511
27 LG:399238,1:2000SEP081213990H 1 1230 1454
27 LG;399238,1;2000SEP085005692H1 1148 1400
27 LG:399238.1:2000SEP082673478F6 1077 1383
27 LG:399238.1;2000SEP08g49911 b5 910 1367
27 LG:399238,1;2000SEP08g3151254 1094 1367
27 LG:399238.1:2000SEP084717584Th 954 1362
27 LG:399238.1:2000SEP084739746Th 1034 1362
27 LG;399238,1:2000SEP083930187F8 813 1357
27 LG;399238,1:2000SEP082102303H 1 1194 1351
27 LG:399238,1:2000SEP082673478H 1 1077 1331
27 LG:399238,1:2000SEP087625076) 1 653 1175
27 LG:399238,1;2000SEP08g770134 993 1170
27 LG:399238,1:2000SEP086316289H 1 997 11 b8
27 LG:399238.1:2000SEP086551645H1 577 1164
27 LG;399238.1:2000SEP083930103F6 813 1152
27 LG:399238.1:2000SEP083784920H1 804 1113
27 LG:399238.1:2000SEP086802648) 1 486 1080
27 LG:399238.1:2000SEP08137215H1 832 1017
27 LG;399238,1:2000SEP083930103H1 813 929
111
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
27 LG;399238.1:2000SEP087625076H1 1 650
27 LG;399238.1;2000SEP084717584F6 135 522
27 LG:399238.1:2000SEP086802648H 2 310
1
27 LG;399238.1:2000SEP083454431 H2 30 282
27 LG:399238.1;2000SEP083293457H1 8 273
27 LG:399238.1:2000SEP084717584H1 135 213
28 LG: l 382945.7:2000SEP086006836H 185 471
1
28 LG:1382945.7:2000SEP084642496T8 1 453
28 LG;1382945.7:2000SEP08464249679 116 572
~
29 LG:i3836i0.3:2000SEP083552107H1 1469 1615
29 LG;1383610.3:2000SEP086815380H 291 707
1
29 LG:1383610.3:2000SEP083321944F6 431 931
29 LG;1383610.3:2000SEP083321944H 431 651
1
29 LG:1383610.3:2000SEP082857659H 434 553
1
29 LG:1383610.3:2000SEP08279555H1 459 518
29 LG:1383610.3;2000SEP08g672824 478 659
29 LG:1383610.3:2000SEP08g672823 478 659
29 LG:1383610.3:2000SEP087035734H 494 640
1
29 LG;1383610.3:2000SEP083925445H 522 718
1
29 LG;1383610.3:2000SEP083925445F6 522 1030
29 LG:1383610.3:2000SEP08682691 H 573 754
1
29 LG:1383610.3:2000SEP084586429H 581 801
l
29 LG:1383610.3;2000SEP085883541 H1 584 859
29 LG:1383610.3:2000SEP08' 5881487H1 584 844
29 LG;1383610.3;2000SEP085890387H1 585 829
29 LG:1383610.3:2000SEP085157959H1 659 928
29 LG;1383b10.3:2000SEP081789327H1 680 927
29 LG:1383610.3:2000SEP086819005H1 742 849
29 LG;1383610.3:2000SEP082569594H 676 928
1
29 LG;1383610.3;2000SEP084691317H1 693 951
29 LG:1383610.3:2000SEP08236264086 737 1258
29 LG;1383610.3:2000SEP082362640Hi 693 934
29 LG:1383610.3;2000SEP084617404H1 700 948
29 LG;1383610.3:2000SEP086964192H1 749 837
29 LG;1383610.3:2000SEP086964292H 741 1138
1
29 LG:138361 0.3:2000SEP085888984H 829 992
1
29 LG:1383610.3:2000SEP085559661H1 765 870
29 LG:1383610.3:2000SEP081368378H1 765 886
29 LG:1383610.3:2000SEP086307006H 766 987
1
29 LG:1383610.3:2000SEP082939651 H 828 926
l
29 LG:1383610.3;2000SEP08164944576 1317 1573
29 LG:1383610.3:2000SEP08539686671 1318 1596
29 LG:1383610.3;2000SEP08g2819781 1326 1624
29 LG: l 383610.3:2000SEP083129583H 1328 1 6l
1 8
29 LG;1383610.3:2000SEP08g2178818 1352 1617
29 LG;1383610.3:2000SEP08g6993030 1388 1615
29 LG;1383610.3:2000SEP08141412576 1 500 1 577
29 LG:1383610.3:2000SEP081414125H 1501 1554
1
112
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
29 LG;1383610.3:2000SEP081414125F6 1501 1615
29 LG;1383610.3;2000SEP085679149H1 1502 1775
29 LG:1383610.3;2000SEP08g 1264772 1528 1619
29 LG:1383b10.3;2000SEP082760888H1 1698 1919
29 LG:1383610.3;2000SEP08276088886 1699 1949
29 LG:1383b10.3;2000SEP085726387H1 1805 1872
29 LG:1383b10.3:2000SEP08276088876 1848 2434
29 LG;1383610.3;2000SEP08g817671 2235 2485
29 LG:1383610.3:2000SEP0809386586 2363 2638
29 LG;1383610.3;2000SEP08g5175836 2448 2600
29 LG:1383610.3:2000SEP083418916H1 291 526
29 LG;1383610.3;2000SEP08g4243476 1300 1 6l
8
29 LG:1383610.3:2000SEP085038137F6 1 429
29 LG;1383610.3;2000SEP085038i37H1 1 236
29 LG:1383b10.3;2000SEP081639525H1 1146 1339
29 LG:1383610.3;2000SEP08g5435989 1223 1622
29 LG:1383610.3;2000SEP085792723H1 1223 1519
29 LG:1383b10.3:2000SEP08g3923642 1240 1622
29 LG;1383610.3;2000SEP08266860876 1251 1578
29 LG;1383b10.3:2000SEP08g1055507 1279 1617
29 LG;1383b10.3:2000SEP08g3095471 1296 1622
29 LG:1383b10.3;2000SEP081639525F6 1204 1598
29 LG:1383610.3;2000SEP08g2617139 1469 1615
29 LG:1383610.3:2000SEP08g2553584 1145 1583
29 LG:1383610.3;2000SEP08'5487273H1 1143 1389
29 LG:13836i0.3;2000SEP08043657H1 1087 1391
29 LG:1383610.3:2000SEP0899019371 l 1 b0 1577
29 LG:1383610.3;2000SEP083959408H1 1163 1451
29 LG;1383b10.3:2000SEP08g2824450 1174 1621
29 LG:1383610.3;2000SEP08g4136922 1175 1618
29 LG:1383610.3;2000SEP08g1727608 1180 1618
29 LG:1383b10.3;2000SEP08503813776 1180 1732
29 LG;1383610.3:2000SEP08g3933824 1181 1623
29 LG:1383610.3;2000SEP08163952576 1197 1578
29 LG:1383610.3:2000SEP08g2265436 1555 1615
29 LG;1383610.3:2000SEP085175102F6 838 1322
29 LG;1383610.3;2000SEP085175102H1 838 966
29 LG:1383610.3:2000SEP085613424H1 817 1058
29 LG:1383610.3:2000SEP084638701 H 820 1082
1
29 LG;1383610.3;2000SEP083904031 H 828 1112
1
29 LG:1383610.3:2000SEP084229042H1 863 1129
29 LG;1383610.3:2000SEP086817269H 914 1315
1
29 LG;1383610.3:2000SEP083164238H 925 1188
1
29 LG:1383610.3:2000SEP084443014H 927 1203
1
29 LG;1383610.3:2000SEP084856855H 928 1183
1
29 LG:1383610.3:2000SEP084081134H 939 1183
1
29 LG:1383610.3:2000SEP086717283H1 999 1496
29 LG:1383610.3:2000SEP08332194476 1007 1564
113
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
29 LG;1383610.3:2000SEP086352656H 953 1239
1
29 LG:1383b10.3:2000SEP086315818H1 1061 1320
29 LG:1383610.3:2000SEP08357758H 1 984 1229
29 LG: T 3836 T 0.3:2000SEP084369314H 950 1155
T
29 LG:1383b10.3;2000SEP08476559H1 998 1274
29 LG:1383610.3:2000SEP083902993H1 1033 1296
29 LG:1383b10.3:2000SEP08259131 F1 1034 1615
29 LG:13836T0.3;2000SEP086067265H1 1038 1589
29 LG;1383610.3:2000SEP08904451 H 1006 1276
1
29 LG:1383b10.3;2000SEP082668608F6 1054 1545
29 LG;1383610.3;2000SEP084320682H1 1002 1261
29 LG:1383610.3;2000SEP085287774H1 1004 1258
29 LG:1383610.3:2000SEP08236264076 1066 1573
29 LG;1383610.3:2000SEP08g 1423037 1011 1325
29 LG:1383610.3;2000SEP08938741 R1 1073 1587
29 LG;1383610.3:2000SEP08938741 T1 1073 1571
29 LG;1383610.3:2000SEP08938741 H 1015 1297
1
29 LG:1383610.3:2000SEP08g2030629 1015 1351
29 LG;1383b10.3;2000SEP085741851H1 1017 1302
29 LG;1383610.3:2000SEP084460572H1 1017 1278
29 LG;1383610.3:2000SEP081923660H1 1022 1297
29 LG;1383610.3;2000SEP08192366076 1080 1576
29 LG:1383610.3;2000SEP08192366086 1080 1585
29 LG;13836T0.3;2000SEP085730070H1 7082 1615
29 LG:1383b10.3:2000SEP083483646H1 1132 1447
29 LG:1383b10.3:2000SEP08225790786 1087 1577
29 LG;1383610.3;2000SEP082257907H1 1087 1323
29 LG;1383610.3:2000SEP081238605H 1031 1178
1
29 LG:1383b10.3:2000SEP081792952H1 1033 1307
29 LG:1383610.3;2000SEP08156151276 1096 1564
29 LG:1383610.3:2000SEP082409081H1 1052 1294
29 LG;1383610.3:2000SEP08517510276 1117 1605
29 LG:1383610.3:2000SEP08g2553606 1124 161
b
29 LG:1383610.3:2000SEP08225790776 1132 1593
29 LG;1383610.3:2000SEP083369566H1 1079 1359
29 LG:1383b10.3:2000SEP08g2958446 1143 1624
29 LG:1383610.3:2000SEP086053157) 798 1369
1
29 LG:1383610.3;2000SEP082745126H1 794 1024
29 LG;1383610.3:2000SEP087620203) 813 1367
1
29 LG:1383610.3:2000SEP084717276H1 767 845
29 LG;1383610.3:2000SEP086739535H 736 7299
1
29 LG;1383610.3:2000SEP082235421H1 1540 1615
29 LG:1383610.3:2000SEP08g2725931 1476 1615
29 LG:1383610.3:2000SEP086405358H1 1547 1615
29 LG;1383b10.3;2000SEP082234402H1 1540 1615
29 LG:1383610.3:2000SEP082241280H1 1540 1615
30 LG:1384030.1:2000SEP086777156H 365 873
1
30 LG:1384030.1;2000SEP086456081 H 378 945
1
114
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
30 LG;1384030.1:2000SEP08g813592 521 841
30 LG: i 384030.1:2000SEP081241458H 623 859
1
30 LG:1384030.1:2000SEP083232547H1 636 856
30 LG:1384030.1;2000SEP087740771 J bbl 1204
1
30 LG;1384030,1:2000SEP084226494H1 1925 2192
30 LG:1384030.1:2000SEP08279281776 1610 2152
30 ~LG:1384030.1;2000SEP08g1156926 1945 2192
30 LG;1384030.1;2000SEP08228462376 1975 2440
30 LG:1384030,1;2000SEP083084794H1 2008 2284
30 LG:1384030.1:2000SEP08g3149789 2021 2489
30 LG;1384030.1:2000SEP082685272H1 2048 2192
30 LG:1384030,1;2000SEP08g2955029 1752 2063
30 LG:1384030.1;2000SEP08g3962206 1755 2198
30 LG:1384030,1:2000SEP08766404H 1 1763 2008
30 LG:1384030.1:2000SEP08g2786198 1767 2198
30 LG:1384030,1:2000SEP086365044H1 1794 2063
30 LG;1384030,1:2000SEP08g2215615 1823 2192
30 LG:1384030,1:2000SEP08g 1948592 1370 1648
30 LG:1384030.1:2000SEP08g 1948812 1399 1675
30 LG:1384030.1:2000SEP086442338H1 1434 1968
30 LG:1384030,i;2000SEP08744753672 1486 2085
30 LG:1384030,1;2000SEP086777364H 80 303
1
30 LG;1384030.1:2000SEP083855585F6 98 610
30 LG;1384030,1;2000SEP08741974H 1 153 300
30 LG:1384030.1:2000SEP086777364J 80 303
1
30 LG;1384030.1;2000SEP083341227H 155 405
1
30 LG;1384030,1;2000SEP083341227F6 156 777
30 LG:1384030.1:2000SEP086906377H1 357 857
30 LG:1384030.1:2000SEP086778372H 365 946
1
30 LG:1384030.1;2000SEP084228325H1 1925 2192
30 LG;1384030,1:2000SEP08744925472 1501 2113
30 LG;i384030,1;2000SEP083822349H1 1563 1841
30 LG:1384030.1:2000SEP086159726H1 1600 1799
30 LG:1384030,1:2000SEP08334122776 1608 2057
30 LG;1384030.1:2000SEP085173885H1 1097 1347
30 LG;1384030.1:2000SEP085926007H1 1098 1398
30 LG:1384030.1;2000SEP086839205H1 1103 1632
30 LG:1384030.i:2000SEP084953785H1 1250 1497
30 LG;1384030.1;2000SEP087607682J1 1279 1820
30 LG;1384030,1;2000SEP086051418J1 1328 1823
30 LG:1384030.1:2000SEP086051418H1 1328 1795
30 LG;1384030.1:2000SEP085266960H1 1363 1625
30 LG:1384030,1:2000SEP08g2022434 667 903
30 LG:1384030,1:2000SEP08g892844 685 1082
30 LG;1384030.1;2000SEP08g4265266 703 1132
30 LG:1384030,1:2000SEP08g2838388 753 1199
30 LG:1384030.1:2000SEP082792817F6 794 1342
30 LG:1384030.1:2000SEP08g2215668 819 1227
115
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
30 LG:1384030.1:2000SEP083250390H 926 1239
1
30 LG:1384030.1:2000SEP086777156J1 790 1387
30 LG:1384030.1;2000SEP082792817H1 793 1083
~
30 LG:1384030.1;2000SEP087225486H 27 527
1
30 LG:1384030.1:2000SEP081648239H1 28 249
30 LG:1384030.1;2000SEP086482181 H1 63 537
30 LG:1384030.1;2000SEP087607682H 67 401
1
30 LG:1384030.1:2000SEP087236586H 77 459
1
30 LG:1384030.1;2000SEP08g6199353 1945 2196
30 LG:1384030.1:2000SEP08g3230137 i 744 2196
30 LG;1384030.1:2000SEP081601902H1 2095 2294
30 LG:1384030.1;2000SEP081601960F6 2095 2462
30 LG:1384030.1;2000SEP081601960H1 2095 2295
30 LG:1384030.1;2000SEP08281324176 2200 2428
30 LG;1384030.1:2000SEP082813241F6 2207 2466
30 LG:1384030.1:2000SEP082813241H1 2207 2420
30 LG:1384030.1;2000SEP082813110H1 2207 2415
30 LG:1384030.1:2000SEP08160196076 2285 2420
30 LG:1384030.1:2000SEP08g5706950 2073 2473
30 LG:1384030.1;2000SEP08' 1648239F6 28 404
30 LG:1384030. i :2000SEP08486891 H 30 278
1
30 LG;1384030.1;2000SEP083984662H 44 175
1
30 LG;1384030.1;2000SEP08~ 1839976H 45 215
1
30 LG:1384030.1;2000SEP087345515H1 50 582
30 LG:1384030.1:2000SEP083984662F7 b0 619
30 LG:1384030.1;2000SEP08g4005994 1825 2192
30 LG:1384030.1:2000SEP08g5177997 1829 2192
30 LG;1384030.1:2000SEP08g2873855 1828 2192
30 LG:1384030.1;2000SEP08g 1110202 1831 2192
30 LG:1384030.1:2000SEP08g 1 O 14137 1840 2187
30 LG:1384030.1:2000SEP08g4095889 1856 2192
30 LG; i 384030.1:2000SEP08g224 i 937 1862 2191
30 LG:1384030.1;2000SEP08g892736 1884 2208
30 LG:1384030.1:2000SEP08g2541551 1883 2178
30 LG;1384030.1:2000SEP08265663H1 b3 248
30 LG:1384030.1:2000SEP08291443H1 1045 . 1342
30 LG:1384030.1:2000SEP087740771H1 1074 1709
30 LG:1384030.1;2000SEP08279627476 1632 2144
30 LG:1384030.1:2000SEP08228462386 1635 2025
30 LG;1384030.1:2000SEP082284623H1 1635 ~ 1869
30 LG;1384030.1:2000SEP08781773H 1 1711 1953
30 LG;1384030.1;2000SEP08519946H1 1715 1951
30 LG:1384030. i :2000SEP08164823976 1720 2155
30 LG;1384030.1:2000SEP08385558576 1734 2144
30 LG:1384030.1:2000SEP08g3797322 1739 2192
30 LG: i 384030.1:2000SEP08g813504 1916 220
i
30 LG:1384030.1:2000SEP084352811H1 1888 2113
30 LG:1384030.1:2000SEP08__ 2832028H2 1 260
116
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
30 LG;1384030.1:2000SEP085480213H1 23 206
30 LG:1384030.1:2000SEP085478431 H 23 275
1
31 LG:390475.1:2000SEP08373158H 1 1425 1612
31 LG:390475.1:2000SEP08802683H1 1432 1636
31 LG;390475.1:2000SEP08467131H1 1461 1661
31 LG:390475.1;2000SEP08374250H 1 1424 1672
31 LG;390475.1:2000SEP08468011 H 1455 1688
1
31 LG;390475.1;2000SEP085680194H1 1523 1715
31 LG:390475.1;2000SEP082005982H1 1465 1658
31 LG;390475.1:2000SEP08g1203616 1490 1690
31 LG;390475.1:2000SEP081942223H1 1461 1697
31 LG:390475.1:2000SEP081875616H1 1509 1682
31 LG:390475.1:2000SEP081562493H1 1468 1694
31 LG;390475.1:2000SEP083321585H2 1490 1572
31 LG:390475.1:2000SEP08430766H1 1475. 1679
31 LG:390475.1:2000SEP08467859H1 1532 1630
31 LG:390475.1:2000SEP083916709H 1638 1931
1
31 LG;390475.1:2000SEP08g4457738 527 669
31 LG;390475.1;2000SEP08g1384862 537 684
31 LG;390475.1;2000SEP08g1242860 382 665
31 LG:390475.1:2000SEP08g766144 383 634
31 LG;390475.1:2000SEP08067238H1 392 551
31 LG;390475.1:2000SEP08633651 H 399 653
1
31 LG:390475.1:2000SEP081403384H1 399 604
31 LG;390475.1;2000SEP082209992H1 402 510
31 LG:390475.1;2000SEP08g787869 412 660
31 LG;390475.1:2000SEP08g2835653 418 663
31 LG:390475.1:2000SEP08043054H 1 424 709
31 LG;390475.1:2000SEP08g1274002 428 962
31 LG:390475.1:2000SEP08g3677757 443 671
31 ~ LG:390475.1:2000SEP08g3743491 250 438
31 LG:390475.1:2000SEP082923096H 251 474
1 v
31 LG:390475.1:2000SEP083249704H 291 431
1
31 LG:390475.1:2000SEP081338497H1 309 441
31 LG:390475.1;2000SEP083494313H1 325 467
31 LG:390475.1;2000SEP08g2820741 335 701
31 LG;390475.1;2000SEP084714287H1 365 607
31 LG;390475.1:2000SEP08511973H1 372 566
31 LG;390475.1:2000SEP083955111 H1 1182 1456
31 LG:390475.1:2000SEP08275438H1 1239 1434
31 LG:390475.1:2000SEP084539568H 1190 1442
1
31 LG:390475.1;2000SEP083919091 H 1195 1434
1
31 LG:390475.1:2000SEP082840455H 1203 1482
1
31 LG:390475.1:2000SEP08276868H 1 1238 1467
31 LG:390475.1;2000SEP08778666H1 1155 1385
31 LG:390475.1;2000SEP08024765H1 1156 1346
31 LG:390475.1;2000SEP08g723508 1169 1314
31 LG:390475.1:2000SEP08805277H1 1169 1391
117
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
31 LG:390475.1;2000SEP08g844491 1173 1495
31 LG:390475.1;2000SEP08g735070 1173 1416
31 LG:390475.1:2000SEP082290953H 1 1 1 73 1395
31 LG:390475. 1 :2000SEP084295636H 1 1126 1241
31 LG;390475.1:2000SEP086964476H1 1124 1272
31 LG;390475.1;2000SEP0842957 1 5H 1125 1373
1
31 LG;390475.1;2000SEP086519762H1 1150 1246
31 LG:390475.1;2000SEP08g 1357998 1143 1722
31 LG;390475.1:2000SEP082263032H1 1155 1393
31 LG:390475.1; 2000SEP08g6 1 7641 1 68 414
31 LG;390475.1:2000SEP082044802H1 185 354
31 LG;390475.1;2000SEP08g1958504 199 404
31 LG:390475.1:2000SEP08540922H1 223 442
3 1 LG:390475.1;2000SEP082770791 H 248 479
1
31 LG:390475.1;2000SEP08g 1873997 1 177
.
31 LG:390475.1:2000SEP084084288H1 8 189
31 LG:390475.1:2000SEP082174238H1 8 159
31 LG:390475.1;2000SEP081446053H1 9 264
31 LG:390475.1:2000SEP085298475H 1 9 246
31 LG;390475.1:2000SEP084341605H1 17 234
31 LG:390475.1:2000SEP084145764H 1 18 214
31 LG:390475.1:2000SEP0847 1 3341 19 141
H 1
31 LG;390475.1;2000SEP086441361 H 28 1 69
1
31 LG:390475.1:2000SEP081001468H1 40 276
31 LG:390475.1:2000SEP082723346H1 42 281
31 LG:390475.1:2000SEP081450049H 1 58 259
3i LG;390475.1:2000SEP081839414H1 59 293
31 LG;390475.1;2000SEP08g1364559 73 403
31 LG:390475.1;2000SEP08907971 H1 66 283
31 LG:390475.1:2000SEP08g3182476 8b 319
31 LG:390475.1:2000SEP082475265H1 86 306
31 LG:390475.1;2000SEP085965283H 1 109 264
31 LG:390475.1;2000SEP083580918H1 120 403
31 LG;390475.1;2000SEP083296172H1 1 176
3i LG:390475.1;2000SEP082130474H1 1 246
31 LG:390475.1:2000SEP083369963H1 1 237
31 LG:390475.1:2000SEP082494290H1 1 258
31 LG:390475.1;2000SEP083470236H 1 1 2l 1
31 LG:390475.1:2000SEP081839015H 1 1 117
31 LG;390475.1:2000SEP08630829H1 1 262
31 LG;390475.1:2000SEP083051970H1 1 242
31 LG;390475.1:2000SEP083961074H2 1 224
31 LG:390475.1:2000SEP082947052H2 1 215
31 LG:390475.1:2000SEP081 322474H 1 194
1
31 LG:390475.1:2000SEP081466377H1 1 157
31 LG;390475.1:2000SEP081561545H1 1 1b2
31 LG:390475.1:2000SEP083685341 Hi 1 246
31 LG:390475.1;2000SEP08g1336967 811 1059
IlS
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
31 LG;390475. i :2000SEP08g 1303305 812 929
31 LG:390475.1;2000SEP08795097H1 764 930
31 LG;390475.1:2000SEP08499861 H 1 553 671
31 LG:390475.1:2000SEP082857203H1 556 642
31 LG:390475.1:2000SEP08g 1146853 562 700
31 LG:390475.1:2000SEP0834661 32H 578 692
1 ~
31 LG;390475.1:2000SEP08452754H1 1095. 1326
31 LG:390475.1:2000SEP086962745H1 1091 1286
31 LG:390475, 1 :2000SEP08g 1983447 1272 T 512
31 LG:390475.1:2000SEP08302583H1 1272 1511
31 LG;390475.1:2000SEP082279888H1 1271 1517
31 LG:390475.1:2000SEP084354187H1 1267 1504
31 LG:390475.1:2000SEP081225050H 1 1269 1507
31 LG:390475.1:2000SEP08869885H T 1241 T 478
31 LG:390475.1:2000SEP08g918854 1262 1679
31 LG:390475.1;2000SEP082007247H1 1256 1422
3 1 LG:390475.1:2000SEP08g 1025808 1263 1493
31 LG;390475.1;2000SEP085218231 H 1267 1502
1
31 LG:390475.1:2000SEP08674150H1 1240 1497
31 LG;390475.1;2000SEP083877381 N 1307 1 508
1
31 LG:390475.1;2000SEP087650404H2 ~ .1309 1910
31 LG:390475.1:2000SEP082761640H 1 1319 1541
3 1 LG:390475.1:2000SEP081 21 b 133H 1349 1507
1
31 LG;390475.1:2000SEP083208733H1 1336 1591
31 LG:390475.1:2000SEP082489552N 1 1339 1 546
31 LG:390475.1:2000SEP082591840H1 1353 1590
31 LG;390475.1:2000SEP08146677H1 1286 1414
31 LG:390475.1:2000SEP08l9bOb80H1 1288 1487
31 LG:390475.1:2000SEP082298738H1 1297 1548
3l LG; 390475. T 4976830H 1 1294 1451
:2000SEP08
31 LG:390475.1:2000SEP081289679H T 1292 1536
31 LG:390475. 1 :2000SEP082480383H 1 1 1 b8
31 LG:390475.1:2000SEP083697730H1 2 228
31 LG:390475. 1 :2000SEP0824421 O1 H 1 164
1
31 LG:390475.1:2000SEP083267911 N 1 190
1
3i LG:390475.1;2000SEP08g1801609 1 258
31 LG:390475.1:2000SEP082304464H1 1 213
31 LG:390475.1;2000SEP083348740H1 1 201
31 LG:390475.1:2000SEP08g 1395505 1281 1442
31 LG:390475.1:2000SEP08552037H 1 1283 1 510
31 LG:390475. 1 :2000SEP08321116N 1 1 281 1518
31 LG:390475.1;2000SEP084652332N 1 1273 1489
31 LG:390475.1:2000SEP08g 1576768 1274 1437
31 LG;390475.1:2000SEP082532160H1 1279 1530
31 LG:390475.1:2000SEP081413859H1 1276 1527
31 LG:390475.1:2000SEP083992247H1 826 1100
31 LG:390475.1:2000SEP086443848H 1 880 1408
31 LG:390475.1:2000SEP08g 1640782 823 970
_ ___ _ _
.
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
31 LG;390475.1;2000SEP087254474H1 926 1362
31 LG:390475.1;2000SEP086967258H 1 926 i 060
31 LG:390475.1:2000SEP08696231 6H 926 1321
1
31 LG:390475.1; 2000SEP086961986H 1 926 1392
31 LG:390475.1:2000SEP08431342H 1 971 1206
31 LG:390475.1:2000SEP083534814H1 1018 1188
31 LG:390475.1:2000SEP082288879H 1 1041 1278
31 LG:390475.1:2000SEP082410736H1 1070 1303
31 LG:390475.1:2000SEP08g395465 1081 1409
31 LG:390475.1:2000SEP082727033H1 1086 1317
31 LG:390475.1:2000SEP08g 1295586 1414 1 bbl
,
31 LG:390475.1:2000SEP08g 1312999 1415 1672
31 LG:390475.1:2000SEP08530963N i 1406 1624
31 LG:390475.1;2000SEP08855339H1 1396 1634
31 LG:390475.1:2000SEP085962214N 1 1363 1526
31 LG:390475.1:2000SEP08452967H1 1366 1583
31 LG:390475.1:2000SEP081421903H1 1372 1545
32 LG;229105.3:2000SEP087435272Hi 1 596
32 LG;229105.3:2000SEP085835361H1 323 592
32 LG:229105.3:2000SEP087435218H1 1 596
32 LG;229105.3:2000SEP085835361 F6 323 712
33 LG:232578.3:2000SEP08g6663427 1120 1412
33 LG:232578.3:2000SEP08g3279586 i 080 i 417
33 LG;232578.3:2000SEP08g1384308 1026 1417
33 LG:232578.3;2000SEP08g1224514 1068 1416
33 LG:232578.3:2000SEP08g1365326 905 1417
33 LG;232578.3:2000SEP08g3674482 965 1417
33 LG:232578.3:2000SEP08g2810626 1034 1416
33 LG:232578.3:2000SEP08g2901324 957 1416
33 LG;232578.3:2000SEP08g6073460 1272 1417
33 LG;232578.3:2000SEP08g 151 b 131 11 b7 141
b
33 LG:232578.3:2000SEP08g 1390751 1093 1418
33 LG:232578.3:2000SEP08g2583395 970 141
b
33 LG:232578.3;2000SEP08g1712889 1123 ~ 1419
33 LG;232578.3:2000SEP08g3182153 1076 1419
33 LG:232578.3:2000SEP08g 1388644 1126 14 i
8
33 LG:232578.3:200DSEP08g3331087 1162 1418
33 LG:232578.3:2000SEP08g6041357 1120 1418
33 LG:232578.3:2000SEP08g4457829 1310 1419
33 LG:232578.3:2000SEP08g333 i 089 1058 1418
33 LG:232578.3:2000SEP08g4080845 1013 1424
33 LG:232578.3:2000SEP08g5450749 1028 1423
33 LG:232578.3:2000SEP08g3842825 1201 1420
33 LG:232578.3:2000SEP08g5527989 1022 1419
33 LG:232578.3:2000SEP08g5858042 1024 1420
33 LG:232578.3;2000SEP08g2540076 1086 1420
33 LG:232578.3:2000SEP083838381H1 827 1089
33 LG:232578.3:2000SEP084640194H 1 845 1077
120
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
33 LG;232578.3:2000SEP082342771 H 849 1063
1
33 LG:232578.3;2000SEP08386912bH1 814 1030
33 LG:232578.3:2000SEP08557 05686 615 1006
33 LG:232578.3:2000SEP08551056H 1 580 788
33 LG:232578.3:2000SEP08g 1978109 458 764
33 LG;232578.3;2000SEP083475224H1 319 650
33 LG:232578.3;2000SEP085523539H1 569 643
33 LG:232578.3;2000SEP08g2818319 132 489
33 LG:232578.3:2000SEP08725294bH 1 381
1
33 LG:232578.3;2000SEP087252946) 1 381
1
33 LG:232578.3:2000SEP08i 44b4bOH 46 288
1
33 LG:232578.3:2000SEP08g897273 i 167 1400
33 LG:232578.3;2000SEP08181975776 860 1399
33 LG:232578.3;2000SEP08g3178b43 912 1383
33 LG:232578.3;2000SEP08279342876 85b 1378
33 LG:232578.3:2000SEP08g2432b83 960 1371
33 LG;232578.3:2000SEP0837288b4H1 1062 1368
33 LG;232578.3:2000SEP08191534bH ' 1106 13b7
1
33 LG:232578.3:2000SEP08542238378 834 1176
33 LG:232578.3;2000SEP08744755372 690 1334
33 LG:232578.3:2000SEP08278007H i 1004 1334
33 LG;232578.3:2000SEP085980881H1 1003 1317
33 LG:232578.3:2000SEP082048520H 1045 i 301
1
33 LG:232578.3:2000SEP082b21273H 1022 1268
i
33 LG;232578.3;2000SEP08275474H1 1006 1224
33 LG:232578.3:2000SEP08286329576 880 1140
33 LG:232578.3:2000SEP08g 1425453 1093 1409
33 LG:232578.3:2000SEP08549352F1 903 1412
33 LG:232578.3:2000SEP08g3037b24 1243 1409
33 LG;232578.3:2000SEP08g5392b54 1303 1403
33 LG:232578.3:2000SEP08g607b333 1122 1416
33 LG:232578.3;2000SEP08g3754744 1083 1416
33 LG:232578.3:2000SEP08g 1729139 1047 1415
33 LG:232578.3;2000SEP08g45650b8 1341 1416
33 LG;232578.3;2000SEP08g6451170 1159 1416
33 LG;232578.3:2000SEP08g i 425344 1 i 27 1416
33 LG;232578.3:2000SEP08g4074741 1010 1415
33 LG:232578.3:2000SEP082847751 H 1218 1402
1
33 LG;232578.3;2000SEP08g5813374 1000 1087
33 LG:232b78.3:2000SEP08891702H1 9b2 1223
33 LG:232578.3;2000SEP08g6228941 951 1106
33 LG:232578.3:2000SEP086323b71 T8 885 1311
34 LG:1166387.9:2000SEP087684091 H1 1 490
34 LG:116b387.9:2000SEP087682011 H2 1 655
34 LG:1166387.9:2000SEP087207248H 1 608
1
34 LG:1166387.9:2000SEP08' 6963062H1 1 44i
34 LG;11 b6387.9:2000SEP086961001 H 1 312
1
34 LG:1166387.9:2000SEP08721035 i 1 626
H i
_ _ _
,
121- __ _
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
34 LG;1166387.9:2000SEP086966266H 1 1 513
34 LG;1166387.9:2000SEP08720721 OH 1 607
1
34 LG;1166387.9:2000SEP087211091 H 1 586
1
34 LG:1166387.9:2000SEP086289389H2 1 171
34 LG:1166387.9:2000SEP087208863H 1 1 556
34 LG:1166387,9;2000SEP087209326Hi 1 573
34 LG: ) 166387.9:2000SEP083580915H 1 36 324
34 LG:1166387.9:2000SEP087207309H 1 104 588
34 LG:1166387.9:2000SEP084759262F6 136 458
34 LG:116b387.9;2000SEP084759262H1 189 457
34 LG;116b387.9:2000SEP085664088H 1 381 648
34 LG;1166387.9:2000SEP082733526H 1 501 772
34 LG:1166387.9:2000SEP082763906H 1 625 862
35 LG:351357.1:2000SEP0883078297 743 1080
35 LG:351357,1:2000SEP087677606J 1 996 1463
35 LG:351357.1:2000SEP0882577431 9 341
35 LG;351357,1:2000SEP087369934H1 13 605
35 LG:351357.1;2000SEP087370474H1 12 598
35 LG:351357.1:2000SEP0885440203 173 592
35 LG;351357,1;2000SEP087463274H1 291 871
35 LG:351357.1:2000SEP0885440947 1 429
35 LG:351357.1:2000SEP0882553543 2 443
35 LG;351357.1;2000SEP0882590051 6 433
35 LG:351357,1:2000SEP087677606H 1 1 587
35 LG:351357.1;20005EP0881989041 1118 1417
36 LG:465592,1:2000SEP084567709H1 1 272
36 LG;465592,1:2000SEP081897368H 1 i 239
36 LG:4b5592,1;2000SEP084567709F6 82 307
36 LG;465592.1:2000SEP084551518H 1 161 4i 2
36 LG:465592.1;2000SEP0885111406 229 703
36 LG:465592.1:2000SEP084779885H1 5 297
36 LG:465592,1;2000SEP084779885F7 5 501
36 LG:465592,1:2000SEP085207964T6 648 749
36 LG:465592.1;2000SEP08g 1005124 393 656
37 LG:OOb848.5:2000SEP0886462516 353 731
37 LG:OOb848.5:2000SEP085184275H1 626 732
37 LG:006848.5:2000SEP086311035H1 1 579
37 LG:OOb848,5;2000SEP086308035H1 1 489
37 LG;006848.5:2000SEP085824936H1 418 732
37 LG:OOb848.5:2000SEP08550415Th 449 697
37 LG:OOb848.5:2000SEP083715678H1 455 729
37 LG:OOb848.5:2000SEP087679392) 1 361 464
37 LG:006848.5:2000SEP0886036344 343 732
37 LG:006848.5:2000SEP087171363H1 386 732
38 LG:198450.2:2000SEP083765347H 1 1 286
38 LG:198450.2:2000SEP082881536F6 23 509
38 LG:198450.2;2000SEP082881536H1 23 287
38 LG:198450.2:2000SEP083692305H l 226 529
_ . ~2.~__
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: iD
38 LG:198450,2;2000SEP082119916Th 467 1024
39 LG:1008175.1:2000SEP086798057F8 1 565
39 LG:1008175,1:2000SEP086798057H1 1 529
39 LG:1008175.1;2000SEP08679805778 490 1065
40 LG:437981,11:2000SEP087401501 H 1 569
1
40 LG:437981.11;2000SEP083254083H 37 260
1
41 LG;1025549.1;2000SEP08679b976H 1 253
1
41 LG:1025549,1:2000SEP086796976F8 45 582
41 LG;1025549,1;2000SEP08679697678 217 776
42 LG:327226.16:2000SEP08551971 H1 1 121
42 LG;327226.16;2000SEP083019718H1 1 281
42 LG:327226,16:2000SEP083479389H1 26 211
42 LG:32722b.16;2000SEP081696849Th 46 535
42 LG:327226.16:2000SEP0832289827b 273 546
42 LG;327226.16:2000SEP082948548H1 478 588
43 LG:I 387394,5:2000SEP086825507) 1 647
1
43 LG;1387394,5:2000SEP08673356H1 80 226
43 LG:1387394.5;2000SEP087b1227bJ1 151 694
43 LG;1387394,5:2000SEP086825507H1 309 818
44 LG;445188,3;2000SEP085434427H1 1 210
44 LG:445188.3:2000SEP085434427F9 T T 62
44 LG:445188,3;2000SEP087234208H1 40 323
44 LG:445188,3:2000SEP087689303)1 b7 612
44 LG;445188.3;2000SEP082588419H1 106 336
44 LG:445188,3:2000SEP082588419Fb 106 323
44 LG:445188,3:2000SEP081465708H1 21 235
44 LG:445188.3:2000SEP087234146H 39 323
1
44 LG;445188.3;2000SEP08233146486 20 608
44 LG:445188,3;2000SEP0823314b4H1 20 237
44 LG;445188,3:2000SEP087658611 H 15 324
1
44 LG:445188.3:2000SEP085802042H1 7 325
44 LG;445188,3:2000SEP0879408b3H1 1 323
45 LG;898864.11:2000SEP084179195Fb 1 506
45 LG:8988b4,11:2000SEP087734109H2 167 570
45 LG;898864.11;2000SEP08g3401261 321 750
45 LG;898864,11;2000SEP083323281 H 379 632
1
45 LG;898864,11:2000SEP083790088H 386 675
1
45 LG;898864.11;2000SEP0832 T 3979H 527 673
T
45 LG:898864,11:2000SEP084179195H1 1 267
46 LG:018739.2;2000SEP08143b493H 1163 1424
1
46 LG:018739.2:2000SEP08159249H 1 1444 1643
46 LG;018739.2;2000SEP08793 T T 11 1 484
H 1
46 LG:018739,2:2000SEP083522778H1 341 576
46 LG;018739.2:2000SEP0882883522 840 1191
46 LG:018739.2:2000SEP084333260F6 710 1141
46 LG;018739.2:2000SEP085203041 Fb 407 877
46 LG:OT8739,2:2000SEP08819b3365 1077 1572
46 LG:OI 8739.2:2000SEP085042285H 979 127
1 4
123
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
46 LG;018739.2:2000SEP083402768H1 994 1247
46 LG:018739.2:2000SEP084344137H1 1032 1305
46 LG:018739.2:2000SEP084333260N 1 865 1141
47 LG:302915.b;2000SEP086949968H1 193 786
47 LG:302915.6:2000SEP084217462H 1 512 767
47 LG:302915.6:2000SEP084217462F6 512 832
47 LG:302915.b;2000SEP087316858Hi 107 387
47 LG:302915.6:2000SEP085044773H1 1 274
47 LG:3029i5.b:2000SEP085044773F8 1 426
48 LG;404418.3:2000SEP087607614J1 215 804
48 LG:404418.3:2000SEP086009830H 1 233 520
48 LG;404418.3:2000SEP087607614H1 1 541
48 LG:404418.3;2000SEP087714061J1 127 689
48 LG:404418.3:2000SEP085515579H 1 2b7 423
48 LG:404418.3:2000SEP087714061H1 380 923
48 LG:4044i8.3:2000SEP0883231516 446 758
49 LG:374853.2:2000SEP083559850F6 1 530
49 LG:374853.2:2000SEP083559850H1 2 304
49 LG:374853.2;2000SEP088316550 i 93 475
49 LG:374853.2;2000SEP088713154 303 392
49 LG:374853.2:2000SEP087080736H 1 437 939
49 LG:374853.2:2000SEP08694901588 515 1137
49 LG:374853.2;2000SEP086326129H1 533 789
49 LG;374853.2:2000SEP08433967H1 655 862
49 LG:374853.2;2000SEP084891603H1 1049 1144
49 LG;374853.2:2000SEP086933322Hi 1349 1856
49 LG:374853.2;2000SEP081685549Th 1403 1872
49 LG:374853.2;2000SEP083286591 H2 893 1135
49 LG:374853.2:2000SEP083284066H1 895 1140
49 LG:374853.2:2000SEP081685549F6 1024 1499
49 LG:374853.2:2000SEP081685549H 1 1024 1144
49 LG:374853.2:2000SEP081685137H1 1024 1144
50 LG:228930.1:2000SEP083802974H 1 1027 1307
50 LG:228930.1;2000SEP087626122J1 541 1178
50 LG:228930.1:2000SEP084753733H 1 121 279
50 LG:228930, i :2000SEP084753733F8 122 b 13
50 LG:228930.1:2000SEP087065882H1 183 679
50 LG:228930.1:2000SEP0885676063 251 722
50 LG:228930.1;2000SEP081576986F6 270 661
50 LG:228930.1;2000SEP081576986H 1 270 349
50 LG:228930.1:2000SEP081576986Th 275 683
50 LG;228930.1:2000SEP0883843718 364 724
50 LG:228930.1:2000SEP0883750491 408 722
50 LG:228930.1:2000SEP083604635N1 409 584
50 LG:228930.1;2000SEP087738302J1 518 1130
50 LG:228930.1:2000SEP086991066H1 1 190
51 LG:273593.b:2000SEP086772637H1 1 466
51 LG:273593.6:2000SEP083459140H 1 35 283
124
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
51 LG;273593.6:2000SEP083459140F7 58 579
51 LG;273593.b:2000SEP081957812F6 413 838
51 LG:273593.6:2000SEP081957812H1 413 688
51 LG;273593.b;2000SEP081871603H1 511 636
51 LG;273593.6:2000SEP08~3939407H1 626 730
52 LG:008215.1;2000SEP08506018479 906 1498
52 LG;008215.1:2000SEP08506018478 1024 1488
52 LG:008215.1:2000SEP081263165N 1 1204 1346
52 LG;008215.1:2000SEP086357347F8 103 759
52 LG;008215.1:2000SEP086357347H 1 103 301
52 LG:008215.1:2000SEP08635734778 183 726
52 LG:008215.1:2000SEP083916841 H 415 704
1
52 LG:008215.1:2000SEP086860554H 1 11 290
52 LG:008215.1:2000SEP085089859H1 11 300
52 LG:008215.1;2000SEP086780977) 1 1 418
52 LG;008215.1;2000SEP086595552H2 1 448
52 LG:008215.1:2000SEP08g2112516 731 1225
52 LG:008215.1;2000SEP081319927H 1 788 1020
52 LG:008215.1;2000SEP081319927F6 788 1220
52 LG:008215.1;2000SEP085060184H1 848 1130
52 LG:008215.1;2000SEP087090761 H 445 971
1
52 LG;008215.1;2000SEP083916841 F8 433 954
'
53 LG:337160.1;2000SEP08g3239640 1313 1541
53 LG:337160.1:2000SEP08g2080803 1320 1544
53 LG:337160.1:2000SEP08g4269757 1330 1534
53 LG;337160.1:2000SEP08g1421937 1280 1537
53 LG:337160.1:2000SEP083749493F6 6 343
53 LG:337160.1:2000SEP08g 1925336 11 448
53 LG;337160.1:2000SEP08g2106751 105 561
53 LG:337160.1:2000SEP083457353F6 1. 483
53 LG:337160.1:2000SEP083457353H1 1 247
53 LG:337160.1:2000SEP083749493H1 6 294
53 LG:337160.1:2000SEP08g6439253 1077 1540
53 LG;337160.1:2000SEP08g3596807 1110 1534
53 LG:3371 b0.1:2000SEP08g5526629 1109 1539
53 LG:337160.1:2000SEP08g5232677 1075 1539
53 LG;337160.1:2000SEP084402877F6 140 733
53 LG:337160.1;2000SEP084402877H1 135 389
53 LG;337160.1:2000SEP085923530H1 108 394
53 LG:337160.1;2000SEP083664104H1 440 732
53 LG:3371 b0.1;2000SEP08g 1164166 349 674
53 LG:337160.1:2000SEP085189449F8 1 b2 723
53 LG:337160.1:2000SEP08g6837258 1034 1541
53 LG:337160.1;2000SEP08g752491 609 905
53 LG;337160.1:2000SEP08g1422034 686 1027
53 LG:337160.1:2000SEP085490664H1 696 990
53 LG;337160.1:2000SEP08374949376 916 1492
53 LG:337160.1;2000SEP08518944979 973 1433
__ ___
_
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
53 LG;337160.1;2000SEP0851673b8Hi 563 803
53 LG:337160.1;2000SEP0883593694 1073 1447
53 LG;337160.1:2000SEP088752492 1196 1539
53 LG:337160.1:2000SEP0885633598 1272 1538
53 LG:337160.1:2000SEP0884153397 1272 . 1538
53 LG:337160.1:2000SEP083457353T6 1110 1489
54 LG;3950b3.1;2000SEP0883849737 2713 2998
54 LG:395063.1:2000SEP084073057H T 2689 2978
54 LG;3950b3.1:2000SEP084073057F6 2688 2756
54 LG:395063.1:2000SEP084668484H 1 2671 2919
54 LG;395063.1:2000SEP083535582F6 2636 3182
54 LG:395063.1;2000SEP083535582H1 2636 29i
1
54 LG:3950b3.1;2000SEP083b9b54bTb 1915 2460
54 LG:395063.1:2000SEP083484210H1 2476 2809
54 LG;395063.i;2000SEP087093324F8 1378 1771
54 LG:395063.1:2000SEP086785022H 1 36 ~ 340
54 LG:395063. i :2000SEP083699565H 1 T 285
54 LG;395063.1;2000SEP0885054669 2128 2493
54 LG;3950b3.1:2000SEP0883181305 2249 2493
54 LG:3950b3.1:2000SEP082866136H1 2004 2248
54 LG:3950b3.1;2000SEP087093763F8 1397 1771
54 LG;395063.1:2000SEP082674222H1 1886 2108
54 LG:395063. i ;2000SEP083486280H 1 1566 1661
54 LG:395063.1:2000SEP087401964H 1 1654 2194
54 LG:3950b3.1:2000SEP086828352) 1 67 594
54 LG:3950b3. l :2000SEP084i 80711 H 54 201
1
54 LG:395063.1:2000SEP086788461 H 40 474
i
54 LG:3950b3.1;2000SEP087700096H i 254 855
54 LG:395063.1;2000SEP08031180H 1 206 365
54 LG;395063.1;2000SEP08030545H1 141 365
54 LG:3950b3.1:2000SEP087610103H 1 75 603
54 LG;3950b3.1:2000SEP083b9b54bFb 810 1323
54 LG:395063.1;2000SEP086828352H 1 619 i 1
b5
54 LG:395063.1:2000SEP087086817H1 402 950
54 LG:395063.1:2000SEP08~ 1426382H i 240 1511
1
54 LG:3950b3.1:2000SEP087093763H1 1378 1771
54 LG:395063.1:2000SEP085732526H 1 1047 1322
54 LG:3950b3.1:2000SEP083696546H 1 812 1096
54 LG:3950b3, i :2000SEP084668004H i 2426 2525
55 CG:9790b9.4:2000SEP083988928H 1 11 b8 1356
55 LG:979069.4;2000SEP08398892886 1168 1654
55 LG:9790b9.4:2000SEP084320977Hi 1238 1523
55 LG:979069.4:2000SEP083436719H 1 1300 1538
~
55 LG:979069.4;2000SEP083988928T6 1414 1924
55 LG:979069.4:2000SEP084822576H 1 1868 2130
55 LG:979069.4:2000SEP087664878H1 925 1423
55 LG:979069.4:2000SEP088839595 1022 1258
55 LG:9790b9.4:2000SEP085390613H1 1 259
126
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TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
55 LG:9790b9.4:2000SEP085390613F8 1 555
55 LG;9790b9.4;2000SEP087664878J1 364 998
65 LG;979069.4:2000SEP08424877986 543 993
55 LG:979069.4:2000SEP081681947H1 600 814
55 LG:9790b9.4:2000SEP08g2022820 722 1061
56 LG:346663.5:2000SEP083421067H1 127 327
56 LG;34b663.5:2000SEP085088677H 85 337
1
56 LG;346663.5:2000SEP08211828486 1 370
56 LG;346b63.5:2000SEP084616708H1 1b8 331
56 LG:34b663.5;2000SEP08g2241506 172 611
56 LG:346663.5;2000SEP08g2054215 202 587
56 LG:346663.5;2000SEP08g2882171 226 6l b
56 LG:346663.5;2000SEP08g6086765 236 615
56 LG:346663.5:2000SEP08g5547339 241 615
56 LG;346663.5;2000SEP08g3753350 243 615
56 LG;346b63.5:2000SEP08g2054056 253 616
56 LG:346663.5:2000SEP08g6477039 271 618
56 LG:346663.5;2000SEP08g2806480 332 612
56 LG;3466b3.5:2000SEP08053836H1 381 606
56 LG;346663.5:2000SEP08261802H1 88 258
56 LG:346663.5;2000SEP082118284H1 1 266
56 LG:346663.5;2000SEP08857738H1 54 354
56 LG:346663.5:2000SEP086844557H1 89 619
57 LG:3476i 5.1;2000SEP083532722H 1 184
1
57 LG:347615.1:2000SEP083532722F6 1 259
57 LG;347b15.1:2000SEP08353272276 40 560
58 LG:1397067.1:2000SEP08g6568429 39 440
58 LG: i 397067, i 7606974H i 417
:2000SEP08 i
58 LG:1397067.1:2000SEP084112486H1 547 812
58 LG:13970b7.1:2000SEP082207794F6 436 998
58 LG:1397067.1:2000SEP082207794H1 436 705
58 LG: i 3970b7.1:2000SEP082432882H 60 289
i
58 LG:13970b7.1;2000SEP084790250H 243 ' 506
1
58 LG:13970b7.1;2000SEP085095324H1 794 1055
58 LG:1397067.1;2000SEP087002940H1 754 961
59 LG:120675.1:2000SEP08399139386 20 532
59 LG:120675.1:2000SEP085356838H1 1 207
59 LG;120b75.1:2000SEP083191083H1 390 715
59 LG;120675.1:2000SEP08319108386 390 966
59 LG:120675.1:2000SEP083343664H 324 587
1
59 LG:120675. i ;2000SEP08166952H 1 111 413
59 LG;120b75.1:2000SEP086917760H1 137 619
59 LG:120675.1:2000SEP087143843H1 286 867
59 LG:120675.1:2000SEP083274716H1 571 817
59 LG:120675.1:2000SEP08g 1963587 434 837
59 LG:120b75.1:2000SEP08~ g2105985 623 1074
59 LG:120675.1:2000SEP087040182H1 623 1145
59 LG; i 20675.1:2000SEP087674849H2 605 1197
~ . ____________
__-
_
__ 127
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6~ Template ID Component Start Stop
ID NO: ID
59 LG;i20675.T:2000SEP082866547F6 1100 1490
59 LG;120675.1;2000SEP082908672H 804 1071
1~
59 LG:120b75.1:2000SEP08T 257063H 941 1 T
1 74
59 LG: l 20675.1:2000SEP084947527H 943 1 l
1 98
59 LG:120675.1;2000SEP08678 T 420H 980 1559
1
59 LG;120b75. l :2000SEP082813478H 2 291
1
59 LG: i 20675.1:2000SEP082908672F6 804 T 112
59 LG:120675.1:2000SEP08399739376 639 1134
59 LG;120675.1:2000SEP081553725H 756 953
1
59 LG:120b75.1:2000SEP081348958H i 224 1502
1
59 LG:120675.1:2000SEP086421838H1 1307 7575
59 LG:120b75.1:2000SEP086292026H 1136 1370
1
59 LG;120675.1:2000SEP082866547H1 1100 1415
59 LG:120675.1:2000SEP086295036H 1136 1438
1
59 LG:120675.1:2000SEP08g4686492 1334 1581
59 LG:120b75.1;2000SEP087750 T 50H 49 328
1
59 LG:120675.1;2000SEP083991393H1 20 316
59 LG:120675.1:2000SEP08g 1765138 29 93
60 LG;420050.18:2000SEP08b974290Hi 1 479
60 LG;420050.18:2000SEP084246487H 1 63
1
b0 LG;420050.18:2000SEP082720177H1 1 173
60 LG;420050.18:2000SEP08264909376 356 479
60 LG:420050.18;2000SEP08266128676 226 479
b0 LG:420050.18:2000SEP082649093F6 1l4 531
b0 LG:420050.18:2000SEP082720177F6 1 376
b0 LG;420050.18:2000SEP085623343H1 i0 330
60 LG:420050.18:2000SEP082649093H 114 315
1
60 LG:420050.18:2000SEP083454347H1 ' 114 215
61 LG:220495.3:2000SEP085638207H1 1516 1793
61 LG:220495.3;2000SEP08g827423 1530 1731
61 LG:220495.3:2000SEP08607345276 1547 1822
61 LG:220495.3:2000SEP081299193F6 1547 1860
6l LG:220495.3;2000SEP081299193H1 1547 1737
61 LG;220495.3:2000SEP08129919376 1547 1817
6l LG;220495.3;2000SEP082107254H1 1554 1826
6l LG:220495.3:2000SEP08g560355 1646 1860
61 LG:220495.3;2000SEP08g1784513 1688 1808
6l LG:220495.3;2000SEP082223448H1 1720 1856
61 LG:220495.3:2000SEP082660260H7 1745 1991
61 LG;220495.3:2000SEP084857157H1 1776 1981
6l LG:220495.3;2000SEP08g1398375 1108 1516
6l LG:220495.3;2000SEP086917411 H1 11 T 8 1695
61 LG;220495.3:2000SEP08745202471 1202 1717
61 LG:220495.3:2000SEP08744978672 1205 1750
61 LG:220495.3.2000SEP083 T 42603H 1252 T 408
l
6i LG:220495.3;2000SEP083742603F6 1253 1663
6l LG:220495.3:2000SEP08714314378 1275 1687
61 LG:220495.3;2000SEP08g4985474 1288 1713
128
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WO 02/20756 PCT/USO1/27297
TABLE 3
SE62 Template ID Component Start Stop
ID NO: ID
61 LG:220495.3:2000SEP085099033H i 310 1579
1
6l LG:220495.3:2000SEP084147460H1 1316 1507
b 1 LG:220495.3:2000SEP08314260376 i 365 1823
61 LG:220495,3:2000SEP083689942H1 1402 1b79~
61 LG:220495.3:2000SEP08g4394682 1427 1869
61 LG:220495.3:2000SEP08g3418148 1429 1868
61 LG:220495.3:2000SEP08g 1398292 1439 1845
61 ~ LG:220495,3:2000SEP086197152H1 1448 1883
61 LG:220495.3:2000SEP08263096076 1458 1827
61 LG:220495.3:2000SEP08g3424422 1492 1866
b 1 LG:220495.3:2000SEP08g 1139137 836 1180
61 LG:220495.3:2000SEP082555307H1 956 1207
61 LG:220495,3:2000SEP082554584H1 956 1205
6l LG:220495.3:2000SEP08g781616 1019 1303
6l LG:220495.3:2000SEP086937534H1 1045 1646
61 LG:220495,3:2000SEP086092295H1 1104 1361
6l LG:220495.3:2000SEP08g4435582 1 380
6l LG:220495.3:2000SEP086073452H1 72 231
61 LG:220495,3:2000SEP08~7204038H1 148 663
61 LG:220495.3:2000SEP086981431H1 294 824
61 LG:220495,3:2000SEP082630960H1 730 964
61 LG:220495.3:2000SEP082b309bOFb 730 1089
61 LG:220495.3:2000SEP08609082H 1 801 1064
6l LG:220495.3:2000SEP08g564302 804 1002
61 LG:220495.3:2000SEP086572570) 1 436
1
61 LG:220495.3:2000SEP08744778572 1788 2239
b1 LG:220495.3:2000SEP083242659H1 1893 2113.
6l LG:220495,3:2000SEP08g823764 2038 2374
62 LG:274551.1:2000SEP08g4325750 1 103
62 LG:27455 i .1:2000SEP084290049H 1 124
1
62 LG:274551.1:2000SEP08' 4290049F6 1 353
62 LG:274551.1:2000SEP085493752H 172 444
1
63 LG:429658.27:2000SEP08g5110045 203 646
63 LG:429658,27:2000SEP08g5665175 196 645
63 LG:429658,27:2000SEP08g3700373 299 642
63 LG:429658.27:2000SEP08g300i5i4 414 640
b3 LG:429658.27:2000SEP08g3835002 361 639
b3 LG:429b58.27:2000SEP08g 1367049 263 638
63 LG:429b58.27:2000SEP08g2i 10578 472 639
63 LG:429b58.27:2000SEP08g 1367140 292 625
63 LG:429658,27:2000SEP085825096H1 202 557
63 LG:429b58.27:2000SEP0894389481 1 539
63 LG:429658,27:2000SEP085825196H1 202 521
b3 LG:429b58.27:2000SEP08g1502057 260 460
b3 LG:429658.27:2000SEP08g3118005 249 457
b3 LG:429b58.27:2000SEP08g3041363 195 457
b3 LG:429658,27:2000SEP08g3048788 190 457
b3 LG:429b58.27:2000SEP08943894H 1 1 302
_ -129
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE62 Template (D Component Start Stop
ID NO: ID
b4 LG:246194.18:2000SEP086944445H 702 1175
1
64 LG;246194.18:2000SEP085028768H1 723 995
64 LG:246i 94.18;2000SEP08i 339502H 750 1023
i
64 LG:246194.18:2000SEP0843i7877H1 751 956
64 LG:246194,18:2000SEP081671388H1 703 782
64 LG:24b194.18:2000SEP081671264H1 703 910
64 LG:246194.18:2000SEP08502877bH1 723 991
b4 LG:246194.18:2000SEP082365229H 767 1007
1
64 LG:24b194.18:2000SEP08030557H1 7b8 896
64 LG:246194.18:2000SEP0803 i 790H 774 1007
1
64 LG;246194,18:2000SEP08031792Hi 774 1022
64 LG;246194.18;2000SEP0803194bH1 774 96b
b4 LG:246194.18:2000SEP08031427H 1 774 974
64 LG:24b194.18:2000SEP08032531H1 774 1025
64 LG:246194.18:2000SEP08031b08H1 774 995
64 LG:24b194.18:2000SEP08030659H1 774 950
b4 LG;246194. i 8:2000SEP08g 1270439 780 1 i
74
64 LG;246194.18;2000SEP08g 1241966 798 1082
b4 LG:24b194.18:2000SEP0881990977 798 982
64 LG;246194,18:2000SEP088981948 812 1087
b4 LG:24b194.18:2000SEP084700087H1 820 1076
64 LG:246194.18:2000SEP088749487 838 904
64 LG:24b194.18:2000SEP086744795H1 840 1422
64 LG:246194.18:2000SEP088711338 888 1160
64 LG;246194.18:2000SEP08871b153 898 1195
64 LG:24b194.18:2000SEP082720633H1 931 1164
64 LG:24b194,18:2000SEP08167b222H1 969 1178
64 LG;24b194.18:2000SEP085810107H1 9b9 1253
64 LG:246194.18:2000SEP08l 675139H 9b9 1175
1
64 LG;246194.18:2000SEP08b1b9319H1 969 1268
b4 LG:246194.18:2000SEP088828043 1033 1288
b4 LG:24b194.18:2000SEP085818587H1 1057 1163
64 LG:246194.18:2000SEP085818729H 1057 i 375
1
64 LG;246194.18:2000SEP085814910H1 1057 1366
64 LG:24b194.18:2000SEP085813775H1 1057 1359
64 LG:24b194.18;2000SEP085i88360H1 584 856
64 LG:24b194.18:2000SEP0881281006 b74 1140
64 LG:246 i 94. i 8869772 695 1019
8:2000SEP08
b4 LG:246194,18:2000SEP082888196H 1 278
1
64 LG;24bi94.18:2000SEP08288819bF6 1 5b2
64 LG;24b194.18:2000SEP08173997bRb 2 71
b4 LG:246i94.18:2000SEP081739976H1 3 229
64 LG;246194.18:2000SEP081315b33H1 87 280
64 LG:246194.18:20005EP08079560H 1 b94 852
64 LG:24b194.18:2000SEP084321295H1 699 964
64 LG:246194.18:2000SEP084144711 H1 96 451
64 LG:24b194.18:2000SEP08554b73bH1 2i0 419
64 LG:246194, i 8:2000SEP086450480H 237 805
1
__ __ _____
130
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WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ (D Template ID Component Start Stop
NO: fD
64 LG:246194.18;2000SEP084502146H1 241 513
64 LG:24b 194,18:2000SEP081945717H 1 276 513
.64 LG:246194.18:2000SEP083614166F6 363 888
64 LG:246194,18:2000SEP083614166H1 364 bbl
b4 LG;24b194.18:2000SEP085350605H1 385 629
64 LG:246194.18:2000SEP084752634H1 439 694
b4 LG:246194.18:2000SEP083607954H1 445 638
64 LG:24b194.18:2000SEP085260789H1 478 715
64 LG;246194,18:2000SEP08230783886 488 955
64 LG:24bi94.18:2000SEP082307838H1 488 746
64 LG;246194,18:2000SEP08g983783 554 908
64 LG:246194,18:2000SEP087936779H 1 566 960
64 LG:24b194.18:2000SEP084855732H2 1626 1898
64 LG:246194,18:2000SEP083665761H1 1628 1918
64 LG:246194.18;2000SEP086433267H1 1627 1935
b4 LG;24b194.18;2000SEP084367924H1 1630 1888
b4 LG;24b194.18:2000SEP084368189H1 1630 1875
64 LG;24b194.18;2000SEP081732794Th 1633 2006
b4 LG:246194.18:2000SEP08g718180 1638 1988
64 LG:24b194.18:2000SEP08g5592464 1638 2045
64 LG:246194.18:2000SEP08g4189341 1647 2045
64 LG:246194.18:2000SEP084500190H1 1649 1903
64 LG;246194.18:2000SEP08g1242740 1653 2046
64 LG;24b194,18;2000SEP08g2183532 1656 2043
b4 LG;24b i 94,18:2000SEP08g51 i 0196 1657 2098
64 LG;24bi94.18;2000SEP08g2335977 1659 2046
64 LG:246194.18:2000SEP08g T 267649 1661 2046
64 LG:24b194.18:2000SEP08g5530876 167i 2045
64 LG:24b194.18:2000SEP08g6133595 1674 1935
b4 LG:246194.18:2000SEP08g5529894 1674 2045
64 LG:24b194.1~8;2000SEP08g1226406 1679 2045
b4 LG;24b194.18:2000SEP08g4452780 1683 2045
64 LG:24b194.18:2000SEP08g1024458 1688 2044
b4 LG:24b194.18:2000SEP08g4295379 1692 2045
b4 LG:24b194.18:2000SEP084001495H1 1691 1856
64 LG;246194.18:2000SEP08g982479 1729 2038
b4 LG;246194,18:2000SEP08g3245992 1725 2053
b4 LG:246194,18:2000SEP08g341 6820 1732 2045
64 LG:24b194.18:20DOSEP08g1990978 1738 2045
b4 LG:246194.18:2000SEP08g3412158 1743 2051
64 LG;246194.18:2000SEP08g1027353 1744 2013
b4 LG:24b194.18:2000SEP08g1267651 1746 2046
64 LG:246194.18:2000SEP08g5528370 1763 2045.
b4 LG:246194.18:2000SEP08g6471524 1771 2045
b4 LG:246194.18:2000SEPD81929565Th 1793 2005
64 LG:246194.18:2000SEP08g2819147~ 1799 2045
b4 LG:246194,18:2000SEP08g 1209946 1800 2046
64 LG:24b194.18;2000SEP081929565F6 1804 2043
131
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
T!\BLE 3
SEQ ID Terniplate ID Component Start Stop
NO: ID
64 LG:246194.18:2000SEP08192942416 1804 2016
b4 LG:246194.18:2000SEP081929565H1 1804 2049
b4 LG:246194.18:2000SEP0883736234 1842 2046
64 LG;246194.18:2000SEP0882433089 1863 2050
b4 LG:246194.18:2000SEP0886398179 1872 2048
b4 LG:246194.18:2000SEP088718977 1881 2045
64 LG:246194.18:2000SEP088869773 1884 2055
64 LG:246194.18:2000SEP088749488 1890 2048
b4 LG:246194.18:2000SEP088734528 1908 2028
b4 LG:246194.18:2000SEP088981893 1921 2039
64 LG:246194.18:2000SEP088821354 1939 2043
b4 LG:246194.18:2000SEP0883422981 1560 1987
64 LG:246194.18:2000SEP0883597381 1590 2045
b4 LG:246194.18:2000SEP0885110713 1603 2045
b4 LG:246194.18:2000SEP085836193H1 1602 1877
b4 LG:246194.18:2000SEP088716154 1615 2037
64 LG:246194.18:2000SEP085763563H1 1612 2045
64 LG:246194.18;2000SEP0884175431 1613 2048
64 LG:246194.18:2000SEP0884327368 1615 1994
64 LG:246194.18:2000SEP08833671 b5 1618 2051
64 LG:246194.18:2000SEP0883446877 1621 2050
64 LG:246194.18:2000SEP0882879096 1622 2045
b4 LG:246194.18:2000SEP0886698474 1622 1986
64 LG:246194.18:2000SEP0839204876 1364 1942
b4 LG:246194.18:2000SEP086728329H1 1372 1979
64 LG:246194.18:2000SEP0827716286 1373 1854
64 LG:24b194.18:2000SEP0882178308 1385 1457
b4 LG:246194.18:2000SEP087242736H1 1431 1989
b4 LG:246194.18:2000SEP0883917453 1477 1935
b4 LG:246194.18:2000SEP087589751H1 1484 2021
b4 LG:246194.18:2000SEP0885886998 1487 1935
b4 LG:246194.18:2000SEP08173271376 1501 2002
b4 LG:246194.18:2000SEP084184039H1 1503 1789
64 LG:246194.18:2000SEP0884188392 1529 1987
b4 LG:246194.18:2000SEP0882189470 1550 1939
b4 LG:246194.18:2000SEP082612692H1 1556 1789
b4 LG:246194.18:2000SEP0883596759 1556 1987
b4 LG:246194.18:2000SEP084664359H1 1675 1921
64 LG:246194.18:2000SEP0882694553 1679 2045
64 LG:246194.18:2000SEP085816073H1 1057 1350
64 LG:246194.18:2000SEP085814685H1 1057 1359
64 LG:246194.18;2000SEP085813026H1 1057 1346
64 LG:246194.18:2000SEP085816684H1 1057 1349
64 LG:246194.18:2000SEP085817065H1 1057 1306
64 LG:24b194.18:2000SEP085818965H1 1057 1301
64 LG:246194.18:2000SEP085814606H1 1057 1366
64 LG:246194.18;2000SEP088718007 1063 1391
64 LG:246194.18:2000SEP085877327H1 1077 1269
132
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6~ Template ID Component Start Stop
ID NO: ID
b4 LG:24b194,18;2000SEP084553620H1 11 i.5 1383
64 LG:24b194.18;2000SEP085819710H1 1121 1311
64 LG:246194.18:2000SEP08g734631 1153 1269
64 LG;246194.18:2000SEP08g2185031 1168 1304
b4 LG:246194,18;2000SEP085592178H1 1180 1436
64 LG:246194.18:2000SEP081949202H1 1179 1436
b4 LG;246i94,i8;2000SEP084861510H1 1207 1476
b4 LG:246194.18:2000SEP086213288H1 1328 1480
64 LG:246194.18:2000SEP08361416676 1348 2000
65 LG:000874.1:2000SEP08417806476 i 870 2249
65 LG;000874.1:2000SEP08g2836242 1892 2253
b5 LG:000874,1:2000SEP08g4147256 1894 2282
65 LG:000874,1:2000SEP08g3678872 1895 2285
65 LG;000874.1;2000SEP084515055H 1904 2152
1
b5 LG:000874.1;2000SEP08676441 R6 1983 2282
65 LG:000874.1;2000SEP08676441H1 1983 2256
b5 LG:000874.1;2000SEP0867644176 1983 2241
65 LG;000874,1:2000SEP08672386H 1 1983 2241
65 LG:000874.1;2000SEP08g6043865 2104 2282
b5 LG;000874.1;2000SEP08g5663173 2145 2274
65 LG;000874.1:2000SEP084178064F6 520 1026
65 LG;000874.1:2000SEP086000160H1 968 1466
65 LG:000874.1;2000SEP086308558Hi 967 1528
65 LG:000874,1;2000SEP086558026H 1399 1961
1
65 LG;000874,1:2000SEP084311435H1 1417 1572
65 LG;000874,1:2000SEP086560545H 1429 1940
1
b5 LG:000874.1;2000SEP086445251 T8 1627 2176
65 LG;000874.1;2000SEP08346856476 i 858 2234
a.
65 LG:000874,1;2000SEP083468564H1 1865 2137
65 LG:000874.1;2000SEP083468564F6 1865 2218
65 LG;000874,1:2000SEP087073540H1 i 329
65 LG;000874.1:2000SEP087287283H1 82 396
65 LG;000874.1;2000SEP084711204H 92 226
. 1
65 LG:000874,1:2000SEP086444020H1 95 bll
65 LG;000874.1;2000SEP086445251 F8 339 900
65 LG:000874. i ;2000SEP08644525 i 339 460
H 1
65 LG;000874.1:2000SEP084178064H1 520 770
66 LG:239967.7;2000SEP084137638H1 67 340
~
66 LG:239967,7: 1002437H1 1 226
2000SEP08
66 LG:239967.7:2000SEP086452421 H2 4 321
66 LG:2399b7.7;2000SEP085699293H1 5 196
67 LG:238388.1;2000SEP08g4114i41 3607 3994
67 LG;238388,1;2000SEP08299608176 3611 3957
b7 LG;238388.1:2000SEP085550244H1 3628 3874
67 LG:238388.1:2000SEP084787718H1 3645 3890
67 LG;238388,1:2000SEP081712082H 3647 3849
1
67 LG:238388.1:2000SEP085205979H 3673 3911
1
67 LG:238388.1:2000SEP08865_21971 3741 3961
_ -. _ _
___
- 1 J j
.
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6~ Template ID Component Start Stop
ID NO; ID
67 LG:238388.1;2000SEP08865219H1 3740 3908
b7 LG:238388,1:2000SEP082819356H1 3749 3994
67 LG;238388,1:2000SEP08816218R1 3809 3994
67 LG:238388.1:2000SEP08816218H1 3809 3994
67 LG:238388.1:2000SEP08140689276 3811 3956
67 LG;238388.1:2000SEP081406892F6 3818 3994
67 LG:238388,1:2000SEP081406892H 1 3818 3997
67 LG:238388.1:2000SEP08g2017794 3852 3994
67 LG:238388,1:2000SEP0881621871 3856 3953
67 LG:238388.1:2000SEP08g 1210982 3872 4000
67 LG:238388.1;2000SEP08523420Ni 3936 3994
67 LG:238388,1:2000SEP0823545 l OH 3938 3994
1
b7 LG:238388,1:2000SEP085769009H1 749 1071
b7 LG;238388.1;2000SEP086772048H1 875 1470
67 LG:238388.1;2000SEP08g 1959595 922 1317
67 LG:238388.1:2000SEP086916395H1 1002 1294
67 LG:238388.1:2000SEP083563552H 1 1004 ~ l 310
67 LG:238388.1:2000SEP086916017H1 1005 1294
67 LG;238388,1;2000SEP087bb1482Hi 1098 1671
b7 LG:238388,1:2000SEP084777952H 1 1260 1531
67 LG:238388,1:2000SEP085431473H1 1426 1691
b7 LG:238388.1:2000SEP085431473F6 1426 1731
67 LG;238388.1;2000SEP087371680H) 1453 1844
67 LG;238388,1:2000SEP08g2825556 1497 2004
67 LG;238388,1:2000SEP08479240H 1 1549 1758
67 LG:238388,1:2000SEP08g5809860 1609 1891
67 LG;238388.1:2000SEP08543147376 1640 2165
b7 LG;238388,1;2000SEP082886276N 1 1656 1928
b7 LG:238388.1:2000SEP083788121 H 1797 2072
1
67 LG:238388, l :2000SEP084129226H2 1857 2111
67 LG:238388,1;2000SEP08766i 482) 1860 2408
1
67 LG:238388.1;2000SEP084129490H1 1858 2075
67 LG;238388.1;2000SEP085835769H1 1934 2196
67 LG;238388.1;2000SEP087677342)1 2028 2587
b7 LG;238388,1;2000SEP085727827H l 2057 2563
b7 LG:238388,1:2000SEP085975633H1 2082 2535
b7 LG;238388.1;2000SEP087006547H1 2095 2661
b7 LG;238388. l :2000SEP08g3146134 2211 2487
b7 LG:238388.1;2000SEP086757328H 1 2388 2954
b7 LG:238388.1:2000SEP087737258H1 2481 3065
b7 LG:238388.1:2000SEP082922871 H 2556 2836
l
b7 LG;238388,1:2000SEP085412320H 1 2576 2857
b7 LG:238388.1:2000SEP08382 l 375H 2611 2853
1
b7 . LG:238388.1;2000SEP083821375F6 2612 3062
67 LG;238388.1:2000SEP084337484H 1 2617 2942
67 LG;238388. l :2000SEP08143684676 2629 2852
67 LG:238388.1:2000SEP084219215H 1 2689 2971
b7 LG:238388.1:2000SEP087757483) 1 1 659
_ _ _,
-134 _
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
b7 LG:238388,1:2000SEP08g654095 320 576
b7 LG:238388.1:2000SEP087364320H 1 322 678
67 LG:238388,1:2000SEP086818455H 1 361 915
67 LG:238388.1:2000SEP08g6705027 398 887
67 LG:238388.1:2000SEP087757483H1 465 1078
67 LG:238388.1:2000SEP08g654019 668 1002
67 LG:238388.1:2000SEP083769421 H 675 954
1
67 LG:238388, i :2000SEP087345125H 1 2765 3298
b7 LG:238388.1:2000SEP087748144H1 2765 2975
b7 LG:238388, Z :2000SEP081263237H 1 2807 2976
b7 LG:238388,1:2000SEP087614563H 1 2812 3403
67 LG:238388.1:2000SEP083813395H 1 2812 3071
b7 LG:238388.1:2000SEP086757328) 1 2838 3432
67 LG:238388.1:2000SEP08g2000982 2844 3161
67 LG:238388,1:2000SEP087120315H 1 2846 3275
67 LG:238388.1:2000SEP085798452H1 2693 3156
b7 LG:238388. i :2000SEP08g2505206 2728 2979
67. LG:238388.1:2000SEP08250263676 2895 2976
67 LG:238388.1:2000SEP085561 SbbH 2895 3042
1
67 LG:238388,1:2000SEP085561335H 1 2900 3144
67 LG:238388.1:2000SEP083775391 H 2904 3188
1
67 LG:238388.1:2000SEP08g2008372 2970 3240
b7 LG:238388.1:2000SEP082996036H 1 3002 3188
67 LG:238388.1:2000SEP082996081 H 3002 3195
i
67 LG:238388.1:2000SEP082996081 F6 3002 3347
67 LG:238388,1:2000SEP085743469H1 3002 3276
67 LG:238388.1:2000SEP083559545H1 3002 3199
b7 LG:238388,1:2000SEP081213203H1 3021 3275
67 LG:238388.1:2000SEP083901271H1 3070 3340
67 LG:238388,1:2000SEP081481976H 1 3070 3260
67 LG:238388. i :2000SEP081481976F6 3070 3514
67 LG:238388.1:2000SEP085836014H 1 3085 3359
67 LG:238388,1:2000SEP083449721 H 3117 3359
i
67 LG:238388.1:2000SEP083271276H1 3122 3364
b7 LG:238388.1:2000SEP08Ob0887H i 3153 3371
67' LG:238388,1:2000SEP086515184H1 3158 3704
b7 LG:238388.1;2000SEP082020109F6 3241 3680
67 LG:238388,1:2000SEP082020109H 1 3241 3476
67 LG:238388.1:2000SEP082783585H2 3276 3553
67 LG:238388, i :2000SEP08612351 OH 3306 37 i
1 8
67 LG:238388.1:2000SEP085817552H 1 3322 3606
67 LG:238388,1:2000SEP085822881 H 3322 3591
1
67 LG:238388.1:2000SEP083526793H1 3322 3528
b7 LG:238388.1:2000SEP081415243H1 3327 3586
b7 LG:238388.1:2000SEP087329748H1 3343 3949
67 LG:238388.1:2000SEP08009867H1 3346 3668
67 LG:238388.1:2000SEP081808041 H 3351 3612
1
67 LG:238388.1:2000SEP081808041 Fb 335i 3753
____.__
~
_
135
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
67 LG:238388.1:2000SEP082878474H 3358 3638
1
67 LG:238388.1;2000SEP083794206H1 3412 3709
67 LG:238388.1:2000SEP082285 i 58H 3418 3580
1
67 LG;238388.1;2000SEP08304i557H1 3441 3722
67 LG:238388.1;2000SEP082133969H 3456 3710
1
67 LG:238388.1:2000SEP08382137576 3471 3958
67 LG:238388.1:2000SEP08202010976 3484 3952
67 LG;238388.1;2000SEP085139405H 3487 3752
1
67 LG:238388.1:20005EP08886271 Hl 3488 3788
b7 LG:238388.1:2000SEP081969228Hi 3496 3771
67 LG:238388.1;2000SEP08196922886 3496 3962
67 LG:238388.1:2000SEP08196922876 3496 3941
67 LG:238388.1:2000SEP084207304H1 3528 3747
67 LG:238388.1;2000SEP08g5i 10635 3531 3995
67 LG:238388.1:2000SEP08g665b8i9 3533 3994
b7 LG:238388.1:2000SEP084200482H 3534 3837
1
67 LG:238388,1:2000SEP08g3889867 3566 3984
67 LG:238388.1:2000SEP08180804176 3565 3962
67 LG:238388.1:2000SEP08g4850515 3579 3994
67 LG;238388.1:2000SEP08148197676 3593 3932
68 LG:233674,4;2000SEP08g3048238 2946 3414
68 LG:233674.4:2000SEP08g2820354 2909 3415
b8 LG:233674.4;2000SEP08195492476 2910 3380
b8 LG:233674.4:2000SEP087335679H1 2918 3430
68 LG:233674.4:2000SEP08626535H1 2922 3164
68 LG:233b74,4:2000SEP0862653586 2922 3413
68 LG:233674.4:2000SEP086599347H1 2932 3414
68 LG:233674,4;2000SEP08g5593063 2933 3420
68 LG;233674,4:2000SEP08347940H1 2932 3059
68 LG:233674.4;2000SEP08g666613 2948 3412
68 LG:233674.4:2000SEP08g6702571 2942 3414
b8 LG:233b74,4:2000SEP086343858H1 2943 3214
68 LG;233674.4:2000SEP086316286H1 2943 3121
68 LG:233674.4:2000SEP082598676H1 1647 1909
68 LG:233674.4;2000SEP082598676F6 1647 2146
68 LG;233b74.4:2000SEP087711995) 1667 2262
1
68 LG:233b74.4:2000SEP08g564225 1730 1960
68 LG:233b74.4:2000SEP084346248N 1731 2018
1
68 LG:233674,4:2000SEP08666846H 1 1757 2030
68 LG:233674,4:2000SEP082749327H1 1769 2023
b8 LG:233b74,4:2000SEP086798844H1 1889 2448
68 LG:233674.4:2000SEP08469885H 1 1890 2050
68 LG:233674.4:2000SEP08g5837868 2954 3425
68 LG:233674.4:2000SEP08g6986716 2960 3414
68 LG:233b74,4:2000SEP08g4311811 2960 3414
68 LG;233674.4;2000SEP08g5177329 2961 3392
68 LG:233674.4:20005EP08g6661879 2962 3418
68 LG:233b74,4;2000SEP085329124H1 2696 2943
_______
X36__
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
68 LG:233b74.4:2000SEP08g866548 2708 3015
b8 LG:233674.4:2000SEP082935b30Hi 2708 2976
68 LG;233674.4;2000SEP08g855143 2708 2983
68 LG:233674.4:2000SEP083854205H1 2725 3021
68 LG:233b74.4;2000SEP082755008H1 2727 2906
68 LG:233b74.4:2000SEP082232604H1 2729 2884
68 LG;233674.4:2000SEP08g1200259 2735 3017
68 LG:233674.4:2000SEP083970611H1 2740 2998
68 LG;233674.4:2000SEP083967950H1 2753 3000
68 LG;233b74.4:2000SEP084542927H1 2757 3019
68 LG;233674.4;2000SEP083967941 H1 2762 3000
68 LG:233674.4;2000SEP08527407079 2781 3319
68 LG;233674.4;2000SEP082831b82Hi 2818 3077
68 LG:233b74.4:2000SEP08211860476 2837 3385
68 LG:233674.4:2000SEP08g667071 2841 3729
68 LG:233674.4;2000SEP084379465H1 2855 3133
68 LG;233b74.4:2000SEP08642755F1 2882 3418
68 LG;233674.4;2000SEP085703055F6 463 677
68 LG:233b74.4;2000SEP086806348F8 472 1133
68 LG;233674.4:2000SEP08642752H1 83 301
68 LG:233b74.4:2000SEP086920944H 1 110 500
68 LG;233b74.4:2000SEP085379362H i 452 714
68 LG;233b74.4:2000SEP085703055H1 463 592
68 LG:233674.4:2000SEP086806348H 1 472 986
68 LG;233674.4:2000SEP084969531H1 495 699
68 LG;233b74.4:2000SEP085972826H1 550 1068
b8 LG;233674.4;2000SEP08g1976170 551 936
68 LG;233b74.4;2000SEP085972826F8 553 1140
68 LG:233b74.4;2000SEP086829707J1 578 1096
68 LG:233674.4:2000SEP08g2254998 3146 3414
b8 LG:233674.4;2000SEP08g21130i6 3158 3414
68 LG:233b74.4:2000SEP08g4326422 3163 3415
68 LG;233b74.4:2000SEP08g4086677 31 b5 3415
b8 LG:233b74.4;2000SEP0862653576 3135 3375
68 LG:233b74.4:2000SEP08g 1940748 3135 3414
68 LG;233b74.4:2000SEP083154259H1 3146 3410
68 LG;233b74.4:2000SEP08550108Hi 3117 3374
68 LG:233674.4:2000SEP086569734H 1 3118 3663
68 LG;233674.4:2000SEP08334525H1 3123 3358
68 LG:233674.4;2000SEP08637277iH1 3132 3405
68 LG;233674.4:2000SEP08g2058783 3135 3404
68 LG:233b74.4:2000SEP084858527H1 2690 2894
b8 LG:233674.4:2000SEP08548931 OH 2696 2980
1
68 LG:233674.4:2000SEP08g2154333 2681 3090
68 LG:233674.4:2000SEP086325343H 1 2648 2877
68 LG:233674.4:2000SEP081383313H 1 2682 2936
68 LG;233674.4;2000SEP086356947H1 2584 2794
68 LG:233674.4:2000SEP081954924H 1 2486 2708
137
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
b8 LG:233b74.4:2000SEP086724634H 1 2499 3021
68 LG:233b74.4:2000SEP086309069H 1 2547 3125
68 LG:233b74.4:2000SEP08g 1887548 2564 2919
b8 LG:233674,4:2000SEP081726341 H1 2561 2774
68 LG:233b74.4:2000SEP081726341 Fb 2561 3091
b8 LG:233b74.4:2000SEP081727741H1 2561 2774
68 LG:233b74,4:2000SEP081727741 F6 2561 2979
68 LG:233674.4:2000SEP086155590H 1 2575 2876
68 LG:233b74.4:2000SEP08968911 H 1 3181 3412
b8 LG:233674.4:2000SEP0882904859 3185 3418
68 LG:233674,4:2000SEP0884568372 3203 3413
68 LG:233674.4:2000SEP082743761 T6 3212 3354
68 LG:233674.4:2000SEP0882718646 3274 3436
68 LG:233b74.4:2000SEP0882141561 3279 3410
68 LG:233674.4:2000SEP085b03bbbH1 2466 2723
68 LG:233674.4:2000SEP081954924F6 2486 2808
68 LG:233b74,4:2000SEP084851872H1 2486 2666
b8 LG:233674.4:2000SEP082933230H2 2387 2652
b8 LG:233b74,4:2000SEP084635863H1 2407 2668
b8 LG:233674.4:2000SEP08217483H1 2421 2595
b8 LG:233674,4:2000SEP08218920H1 2421 2650
68 LG:233674.4:2000SEP085395195H 1 2428 2633
b8 LG:233674.4:2000SEP085275658H 1 2432 2628
68 LG:233b74.4:2000SEP084876783H 1 2447 2726
68 LG:233b74.4:2000SEP08031485H1 2453 2625
b8 . LG:233b74,4:20005EP085611850H1 2461 2670
b8 LG:233674.4:2000SEP08i43418H1 2463 2687
b8 LG:233b74,4:2000SEP082201263H1 3092 3352
b8 LG:233674.4:2000SEP0885769561 3093 3429
68 LG:233b74.4:2000SEP0886698727 3091 3414
68 LG:233674.4:2000SEP088866438 3096 3414
68 LG:233b74,4:2000SEP086383782H 1 3100 3359
b8 LG:233674.4:2000SEP0883240885 3101 3422
68 LG:233674,4:2000SEP088855630 3104 3388
68 LG:233b74.4:2000SEP0885446385 3110 3414
68 LG:233b74.4:2000SEP08g 1148830 3111 3418
68 LG:233674,4:2000SEP0882191 69b 3112 3413
68 LG:233b74.4:2000SEP0882874136 3115 3413
68 LG:233b74,4:2000SEP088560291 3i66 3414
b8 LG:233674.4:2000SEP0882053192 31 b9 3418
68 LG:233674.4:2000SEP081322413H1 2212 2449
68 LG:233b74.4:2000SEP082743761H7 2219 2489
b8 LG:233674.4:2000SEP082743761 F6 22 i 9 2764
68 LG:233b74.4:2000SEP085087795H1 2223 2488
68 LG:233b74.4:2000SEP087764777H1 2242 2717
68 LG:233674.4:2000SEP085616170H1 2334 2630
b8 LG:233b74.4:2000SEP085662688H1 2368 2567
68 LG:233674.4:2000SEP083643051 H 2372 2647
1
~~s-.
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template iD Component Start Stop
NO ID
68 LG:233b74.4:2000SEP0820801 68H 3738 4027
1
68 LG;233674.4:2000SEP084570665H1 3828 4045
68 LG;233b74.4:2000SEP0884568213 2965 3415
68 LG:233b74.4:20DOSEP0884888371 2968 3419
b8 LG:233674.4;2000SEP083285729Th 2969 3375
68 LG:233674.4:2000SEP0884991299 2970 3406
68 LG:233674.4;2000SEP0885848020 2971 34i
9
68 LG:233b74.4:2000SEP0883751226 2972 3416
68 LG;233b74.4:2000SEP086307793H 1 2975 3410
b8 LG:233674.4:2000SEP0885362584 2975 3396
68 LG;233674.4:2000SEP0883425044 2976 3421
b8 LG;233674.4;2000SEP085043094H1 2979 3228
68 LG:233674.4:2000SEP0886835931 2980 3418
b8 LG:233b74.4:2000SEP0886663640 2984 3387
68 LG:233674.4:2000SEP08g 1939538 2985 34 i
4
b8 LG;233674.4;2000SEP0882840778 2986 3422
68 LG:233674.4;2000SEP0885236328 2991 3414
68 LG:233b74.4:2000SEP0886199120 2997 3414
68 LG:233674.4;2000SEP0885850520 3001 3414
68 LG:233b74.4:2000SEP0883753765 3002 3414
68 LG:233b74.4:2000SEP08. 539519571 3006 3375
68 LG;233b74.4;2000SEP0884691044 3008 3414
68 LG:233b74.4:2000SEP0884649596 3013 3414
68 LG:233b74.4:2000SEP0886399696 3016 3414
68 LG:233b74.4:2000SEP0885394138 301 b 3414
68 LG:233674.4;2000SEP0886402223 3017 3414
68 LG:233b74.4:2000SEP0883245051 3026 3417
68 LG;233674.4:2000SEP086543018H1 3041 3410
b8 LG:233674.4;2000SEP08192736676 3047 3375
68 LG:233b74.4:2000SEP0885765795 3050 3418
68 LG;233674.4;2000SEP0886035660 3049 3418
68 LG:233b74.4:2000SEP084821838H 1 3050 3336
b8 LG:233674.4:2000SEP082835893F6 3052 3461
68 LG:233674.4:2000SEP082835893H1 3052 3313
68 LG;233674.4:2000SEP0886038394 3057 3426
68 LG:233674.4:2000SEP088290021 b 3055 3413
68 LG:233674.4;2000SEP0882768204 3066 3413
68 LG;233674.4;2000SEP081361188F6 3068 3414
68 LG:233b74.4:2000SEP081361072F1 3068 3414
b8 LG:233674.4:2000SEP081361188H 1 3068 3357
b8 LG:233b74.4:2000SEP08136118876 3075 3371
68 LG:233b74.4:2000SEP0882265459 3086 3418
b8 LG:233674.4:2000SEP0883308781 3086 3391
b8 LG:233674.4;2000SEP088880802 3614 3964
b8 LG:233674.4;2000SEP088831073 3616 3965
b8 LG:233674.4:2000SEP088770919 3626 3964
b8 LG:233674.4:2000SEP088874722 3647 3964
68 . LG:233b74.4:2000SEP0884850701 3287 3406
139
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Stark Stop
NO; ID
68 LG:233674.4:2000SEP08g4734601 3289 3406
b8 LG;233674.4:2000SEP084545520H1 3295 3407
68 LG:233b74.4:2000SEP08g2788765 3300 3418
68 LG;233674.4:2000SEP08g1887492 3304 3404
68 LG:233b74.4:2000SEP083349435Hi 3329 3406
68 LG;233b74.4;2000SEP08g6836622 3410 3869
68 LG:233674.4;2000SEP08g4990931 3412 3786
68 LG:233674.4;2000SEP08g315i 731 3412 3801
68 LG:233674.4:2000SEP082735154H 1 3595 3844
68 LG;233674.4;2000SEP082673809H1 3957 4045
68 LG:233674.4:2000SEP082673828H1 3963 4045
68 LG:233674.4:2000SEP086881871H1 3978 4045
68 LG;233674.4;2000SEP084772282F7 1908 2513
68 LG:233674.4:2000SEP084772282H1 1908 2165
68 LG:233674.4:2000SEP084982350H1 1971 2242
68 LG:233b74.4;2000SEP083073533H1 1979 2244
b8 LG:233b74.4:2000SEP087057649N1 2082 2531
68 LG:233b74.4;2000SEP086798844)1 2084 2726
68 LG;233b74.4:2000SEP08g2191282 2108 2416
68 LG;233674.4:2000SEP087239623H1 2203 2496
68 LG:233674.4;2000SEP083285729H1 1309 1566
b8 LG;233b74.4:2000SEP083285729F6 1309 i 891
68 LG:233674.4:2000SEP083599653H 1 1353 1652
~
68 LG;233674.4:2000SEP085617347H1 1437 1651
68 LG:233b74.4:2000SEP08~ i 893894H 1418 1651
1
68 LG;233674.4;2000SEP085800444H1 711 1191
b8 LG:233b74.4:2000SEP084051463H 1 1432 1607
b8 LG:233674.4:2000SEP085274070F9 1443 2084
68 LG:233b74.4:2000SEP087711995H1 984 1604
68 LG:233674.4:2000SEP087744971 J 992 1543
1
68 LG;233b74.4;2000SEP081483611Hi 1106 1351
68 LG:233674.4:2000SEP086197176H1 1302 1815
68 LG;233674.4;2000SEP085274070H1 1443 1685
b8 LG:233b74.4:2000SEP085274070F8 1443 1989
68 LG;233b74.4;2000SEP084051463F8 1444 2021
68 LG:233674.4;2000SEP086534830H1 1525 2050
b8 LG;233674.4:2000SEP087764777)1 1570 2170
68 LG:233674.4;2000SEP086806348)1 1553 2175
68 LG;233674.4:2000SEP087218885H1 1628 2143
68 LG:233674.4:2000SEP086757432F8 1 345
68 LG;233b74.4;2000SEP086757432H1 18 570
68 LG:233674.4:2000SEP083486566H1 49 267
68 LG:233674.4:2000SEP0864275581 83 642
69 LG;411327.2:2000SEP085608336H1 1160 1409
69 LG;411327.2;2000SEP085608336F6 1160 1676
69 LG;411327.2:2000SEP083521640H 1 1285 1457
69 LG:411327.2:2000SEP08352164086 1303 1469
69 LG:411327.2:2000SEP086841761H1 1402 1855
40 -__
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
69 LG:411327.2:2000SEP082417869H 1 1505 1727
69 LG:411327,2:2000SEP082417869F6 1505 1772
69 LG:411327,2:2000SEP086758624)1 1540 2134
69 LG:411327.2:2000SEP081342359F6 1580 2113
69 LG:411327.2:2000SEP087 342359H 1580 1800
1
69 LG:411327,2:2000SEP08g5848294 1651 2110
b9 LG:411327.2:2000SEP08g864162 1772 2090
69 LG:411327,2:2000SEP08g795684 1834 2099
69 LG:411327.2:2000SEP08g3134525 1842 2142
69 LG:411327.2:2000SEP082829095H 1 415 664
b9 LG:411327.2:2000SEP08800329H i 440 686
69 LG:41i327,2:2000SEP08224003iH1 447 699
69 LG:411327,2:2000SEP087462852H1 470 769
69 LG:411327,2:2000SEP087453938H1 606 1167
69 LG:411327,2:2000SEP087529 T 5H 617 894
1
69 LG:411327,2:2000SEP085083742H1 700 924
69 LG:411327.2:2000SEP086758624H 1 815 1390
69 LG:411327,2:2000SEP08300057iH1 1021 1312
b9 LG:411327,2:2000SEP087115241 H1 1136 1685
69 LG:411327.2:2000SEP083336908H1 73 304
b9 LG:411327.2:2000SEP0864260886 110 368
69 LG:411327,2:20005EP08642608H1 110 360
69 LG:411327,2:2000SEP08' 7763655H1 120 746
69 LG:411327,2:2000SEP08803330H1 400 642
b9 LG:411327,2:2000SEP086805668) i i 581
69 LG:411327.2:2000SEP08g1928792 1 296
b9 LG:411327,2:2000SEP083760715H 1 65 384
69- LG:411327,2:2000SEP082106453H1 53 344
b9 LG:411327.2:2000SEP08g1481768 66 374
69 LG:417 327.2:2000SEP08210645386 54 475
69 LG:411327.2:2000SEP08g1163559 61 449
69 ' LG:411327.2:2000SEP083473472H1 66 397
70 LG:1327310.1:2000SEP086795864H 1 1 240
70 LG:13273 i 0,1:2000SEP086795864F8 1 589
70 LG:1327310,1:2000SEP086791830H 1 2 403
70 LG:1327310,1:2000SEP08679586478 74 563
71 LG:242019,13:2000SEP084651672H 1 1 244
71 LG:242019.13:2000SEP087713679)2 24 634
72 LG:012432. i 2:2000SEP082227848 i 558 86 i
72 LG:012432.12:2000SEP08223917H1 558 733
72 LG:012432. i 2:2000SEP0822391786 558 863
72 LG:012432.12:2000SEP08g2620512 582 893
72 LG:012432,12:2000SEP087640335H2 182 783
72 LG:OI 2432,12:2000SEP083271885H 1 i 233
72 LG:OI 2432.12:2000SEP085638620H 1 1 184
72 LG:012432.12:2000SEP08g2 i 11772 1 436
72 LG:012432.12:2000SEP087640335)2 439 847
72 LG:012432, i 2:2000SEP085536760H 1 9 267
141-
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Starf Stop
NO; ID
72 LG;012432.12:2000SEP081484585F6 11 506
72 LG;012432.12:2000SEP081484585H1 11 277
72 LG;012432.12;2000SEP085957017H1 128 642
72 LG:012432.12:2000SEP08222784H1 558 739
73 LG;257088.9;2000SEP08g5542884 1351 1518
73 LG:257088.9:2000SEP08g2840861 1039 1520
73 LG;257088.9:2000SEP082657966Th 567 1061
73 LG;257088.9:2000SEP0881069716 680 1035
73 LG:257088.9;2000SEP084580838H1 772 985
73 LG;257088.9;2000SEP085526482H1 717 964
73 LG;257088.9:2000SEP081705927H1 677 898
73 LG;257088.9:2000SEP0883070773 546 837
73 LG;257088.9;2000SEP082657966H1 531 742
73 LG;257088.9:2000SEP086267885H1 181 688
73 LG;257088.9:2000SEP08bi34084H1 754 964
73 LG:257088.9;2000SEP0883177730 1071 1514
73 LG;257088.9:2000SEP0882782497 1077 1441
73 LG;257088.9:2000SEP0885395430 1052 1432
73 LG;257088.9:2000SEP0881295210 786 1223
73 LG;257088.9;2000SEP086336121H1 563 1076
73 LG;257088.9:2000SEP0885768628 1043 1409
~
73 LG:257088.9;2000SEP088576705i 1008 1388
73 LG:257088.9;2000SEP0885638685 1008 1284
73 LG;257088.9:2000SEP0877b3642Ji 628 1260
73 LG:257088.9;2000SEP087375784H1 1 270
73 LG;257088.9;2000SEP082657966F6 531 1025
73 LG;257088.9;2000SEP086354333H1 677 1001
'
73 LG:257088.9:2000SEP086317132H1 677 956
73 LG;257088.9;2000SEP0883094268 1093 1518
74 LG;997505.5;2000SEP081317853H1 1320 1483
74 LG;997505.5;2000SEP085853247H1 1291 1560
74 LG:997505.5:2000SEP084998506H 1 1 173
74 LG:997505.5;2000SEP084998506F9 1 550
74 LG;997505.5;2000SEP084998506F8 24 521
74 LG;997505.5:2000SEP085528150H 1 488 767
74 LG;997505.5;2000SEP083400869H1 723 974
74 LG;997505.5:2000SEP084740562H2 828 1094
74 LG:997505.5;2000SEP086778477J1 899 1521
74 LG:997505.5:2000SEP088878200 1067 1329
74 LG;997505.5;2000SEP088888053 1070 1382
74 LG:997505.5:2000SEP081391708H i 1153 i 370
74 LG;997505.5;2000SEP084534154F8 1175 1701
74 LG:997505.5:2000SEP08453431 OH 1 i 76 i 421
1
74 LG:997505.5:2000SEP084534310F8 1175 1682
74 LG;997505.5:2000SEP084534154H1 1175 1429
74 LG:997505.5;2000SEP085853215H1 1291 1561
75 LG;481436.2;2000SEP083324046H1 274 573
75 LG:481436.2:2000SEP083635943H 1 16 3 i
2
I42
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
75 LG:481436.2:2000SEP081547119H 1 1 202
75 LG:481436.2:2000SEP086518254H 1 96 609
75 LG:481436.2:2000SEP083635943F6 15 595
75 LG:481436,2:2000SEP087420366T2 455 585
75 LG:481436.2:2000SEP081912239H1 641 882
75 ~ LG:481436.2:2000SEP08g792143 587 870
75 LG:481436,2:2000SEP083332066H1 397 655
75 LG:481436.2:2000SEP08g2725865 663 859
75 LG:481436,2:2000SEP082825487H1 571 859
75 LG:481436,2:2000SEP08g2880948 509 789
75 LG:481436.2:2000SEP084815177H1 537 786
75, LG:481436.2:2000SEP08g3665739 371 783
75 LG:481436.2:2000SEP08g784809 492 779
75 LG:481436.2:2000SEP08g648147 632 781
75 LG:481436.2:2000SEP08g648152 523 781
75 LG:481436.2:2000SEP08g 1812276 427 780
75 LG:481436,2:2000SEP08g2357998 458 780
75 LG:481436.2:2000SEP08g192056i 631 780
75 LG:481436.2:2000SEP08g648i39 515 779
75 LG:481436.2:2000SEP08g 1925882 400 780
75 LG:481436.2:2000SEP08g648137 600 781
75 LG:481436.2:2000SEP08g648140 639 777
75 LG:481436.2:2000SEP085791538H1 633 780
75 LG:481436.2:2000SEP08g858400 474 767
75 LG:481436,2:2000SEP08435556Th 151 742
75 LG:481436,2:2000SEP083812421H1 432 734
75 LG:481436,2:2000SEP08g2767739 583 725
75 LG:481436.2:2000SEP084872051 T7 320 679
75 LG:481436,2:2000SEP08766955H1 745 986
75 LG:481436.2:2000SEP082551976F6 1322 1699
75 LG:481436.2:2000SEP08g 1897690 874 1282
75 LG:481436,2:2000SEP08g856883 963 1271
75 LG:481436.2:2000SEP085726574H1 678 1245
75 LG:481436.2:2000SEP08475140H1 956 1220
75 LG:481436.2:2000SEP086030447H1 909 i 175
75 LG:481436.2:2000SEP087070096N1 1097 1626
75 LG:481436.2:2000SEP08878401H1 1332 1605
75 LG:481436,2:2000SEP082551976H1 1322 1589
75 LG:481436.2:2000SEP085602444H 1 1244 1477
75 LG:481436.2:2000SEP087237776H1 915 1452
75 LG:481436.2:2000SEP08g 1521658 1062 1451
75 LG:481436,2:2000SEP0847514086 956 1349
75 LG:481436,2:2000SEP086339925H1 613 1109
75 LG:481436.2:2000SEP08g809825 864 1097
75 LG:481436,2:2000SEP081912239F6 647 1059
75 LG:481436.2:2000SEP087749017H1 1307 1762.
75 LG:481436.2:2000SEP08400544H 1 1662 1756
75 LG:481436.2:2000SEP08g3595194 1407 1756
143
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6~ Template ID Component Start Stop
)D NO: ID
75 LG;481436.2;2000SEP0884875574 1324 1756
75 LG:481436.2:2000SEP0885391715 1300 1756
75 LG:481436.2:2000SEP08942286H1 1607 1756
75 LG:481436.2;2000SEP08287888976 1418 1852
75 LG;481436.2:2000SEP0886030295 1398 1756
75 LG:481436.2;2000SEP0885885853 1305 1756
75 LG:481436.2:2000SEP08253686776 1383 1852
75 LG:481436.2;2000SEP088812334 1579 1755
75 LG;481436.2:2000SEP0882016599 1404 1750
75 LG;481436.2:2000SEP08i 68443376 1491 i 853
75 LG;481436.2;2000SEP08403054778 1501 1765
75 LG;481436.2:2000SEP0882809664 1359 1749
75 LG;481436.2;2000SEP0884703900 i 299 1705
75 LG:48 i 436.2:2000SEP08255197676 1367 1701
75 LG:481436.2:2000SEP086844365H1 1723 1892
75 LG;481436.2;2000SEP08056667H1 1682 1905
75 LG:481436.2:2000SEP0882881641 1492 1901
75 LG;481436.2:2000SEP088856884 1575 1898
75 LG:481436.2:2000SEP088792144 1572 1892
75 LG:481436.2:2000SEP08g 189769 1528 1892
i
75 LG:481436.2:2000SEP0884875377 1469 1892
75 LG:481436.2;2000SEP0882876435 1480 1892
75 LG;481436.2;2000SEP0885232555 1430 1892
75 LG:48143b.2:2000SEP0882342028 1543 1892
75 LG;481436.2;2000SEP081391226H1 1673 1892
75 LG:48143b.2:2000SEP08363594376 1397 1856
76 LG:247776.14:2000SEP0850227 i 3H 3 i 8 598
1
7b LG:247776.14;2000SEP086298110H1 321 394
76 LG:247776.14:2000SEP086298002H1 322 603
76 LG:247776.14:2000SEP085265311 H2 353 598
76 LG:24777b.14:2000SEP082583722F6 425 876
76 LG:247776.14:2000SEP085390936H1 195 410
7b LG;247776.14:2000SEP084493019H1 316 854
76 LG:247776.14:2000SEP082583722H1 425 651
76 LG:24777b.14:2000SEP082396674H1 549 776
7b LG:247776.14:2000SEP085082245H1 553 802
76 LG;24777b.14:2000SEP087063969Hi 748 984
76 LG;24777b.14:2000SEP083349303H1 1 287
76 LG:247776.14;2000SEP083469636H1 1 228
76 LG:247776.14:2000SEP084606239F6 39 248
76 LG:247776.14:2000SEP086545052H 118 558
1
76 LG:247776.14:2000SEP083967662H1 757 1029
77 LG:008606.14;2000SEP087593063H1 1 586
77 LG:008606.14:2000SEP083559041 H1 99 379
77 LG:008606.14:2000SEP08533589H 1 590 833
77 LG:008bOb.14;2000SEP082343384F6 649 1101
~
77 LG:008b06.14:2000SEP082343384H 649 922
1
77 LG:008b06.14;2000SEP087460317H 817 i 045
1
-.____.__
t44
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
77 LG:008606.14;2000SEP08g 1005379 907 1237
77 LG:008b06.14;2000SEP086994923H1 909 1417
77 LG:008606.14:2000SEP083714147H1 910 1191
77 LG:008b06.14:2000SEP082531438H 915 1194
1
77 LG;008b06.14;2000SEP084969732H1 943 1224
77 LG;008606, i 4:2000SEP082171139H 969 1 i
i 67
77 LG:008606.14:2000SEP08676459H1 974 1250
77 LG:008606.14;2000SEP085803841H1 1062 1365
77 LG:008606.14:2000SEP085803934H1 1062 1353
77 LG:008606.14;2000SEP081574221H1 1063 1293
77 LG;008b06.14;2000SEP081574221F6 1063 1598
77 LG:008606.14:2000SEP085803958H1 1068 1346
77 LG:008b06.14:2000SEP085803950H1 1068 1352
77 LG;008606.14:2000SEP085804133H1 1068 1378
77 LG:008606.14:2000SEP082836642H 410 653
1
77 LG;008606.14:2000SEP085538169H1 461 626
77 LG:008bOb.14:2000SEP083416607H 495 748
1
77 LG;008606.14;2000SEP082607480H1 515 799
77 LG:008606.14;2000SEP085609774H1 533 669
77 LG:008606.14:2000SEP083097711 H 238 387
1
77 LG:008606.14:2000SEP085575416F6 240 801
77 LG:008b06.14;2000SEP085575947F6 240 786
77 LG:008606.14:2000SEP085575416H 240 505
1
77 LG;008606.T4:2000SEP087192474H2 243 846
77 LG:008606.14:2000SEP087192601 H2 246 844
77 LG:008606.14:2000SEP087368491 H 243 656
1
77 LG:008606.14:2000SEP086784752H2 248 354
77 LG:008606.14:2000SEP082489661N1 295 548
77 LG;008606.14:2000SEP085996265H 236 . 456
1
77 LG;008606.14:2000SEP087734608J1 392 994
77 LG;008606.14:2000SEP082836642F6 410 844
77 LG;008606.14:2000SEP083559025H 101 383
1
77 LG:008606.14:2000SEP086380356H 223 536
1
77 LG;008b06.14:2000SEP086381595H1 223 387
77 LG;008606.14:2000SEP083727284H 228 511
1
77 LG:008b06.14;2000SEP082497273H1 230 470
77 LG:008606.14:2000SEP083365462H 230 394
1
77 LG;008b06.14:2000SEP08g55531 b8 233 658
77 LG:008606.14:2000SEP08g6301979 233 661
77 LG:008606.14;2000SEP085676084H1 235 516
77 LG;008b06.14;2000SEP085803849H1 1214 1368
77 LG:008bOb.14:2000SEP08453194H1 1302 1418
77 LG:008606.14:2000SEP081574221 T6 1130 1611
77 LG;008606.14:2000SEP08g4223830 1200 1650
77 LG:008b06.14:2000SEP085804149H1 1068 1373
77 ~ LG;008606.14:2000SEP085803951H1 1069 1350
77 LG:008b06.14:2000SEP082549778H1 1071 1318
77 LG:008606.14:2000SEP0843_61441 1083 1350
H1 _
145
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6? Template ID Component Start Stop
ID NO: ID
78 LG:985092,3:2000SEP082305005H1 1 239
78 LG:985092.3:2000SEP081719078H1 102 336
79 LG:23b649.7:2000SEP086717026F8 14 615
79 LG:236649.7:2000SEP08g 1985898 1 138
79 LG:236649,7:2000SEP086717026H1 1 310
80 LG:245014.2:2000SEP08g3446618 1344 1798
80 LG:245014,2:2000SEP083275925H1 1343 1581
80 LG:245014,2:2000SEP085299476H1 1344 1494
80 LG:245014,2:2000SEP08g2541414 1352 1795
80 LG:245014,2:2000SEP084602871 H1 1366 1613
80 LG:245014,2:2000SEP082884645H1 1373 1636
80 LG:245014,2:2000SEP084196193H1 1468 1602
80 LG:245014,2:2000SEP08g2139409 1482 1872
80 LG:245014,2:2000SEP082872604H1 1485 1746
80 LG:245014.2:2000SEP08g4901006 1507 1567
80 LG:245014,2:2000SEP084212104H1 1532 1800
80 LG:245014.2:2000SEP08960167H1 1655 1778
80 LG:245014.2:2000SEP081420057H 1 199
1
80 LG:245014,2:2000SEP087464312H1 6 106
80 LG:245014,2:2000SEP087006091H1 38 597
80 LG:245014,2:2000SEP086940106H1 118 610
80 LG:245014.2:2000SEP086448760H 262 855
1
80 LG:245014,2:2000SEP084875654H1 312 571
80 LG:245014.2:2000SEP087370769H 410 982
1
80 LG:245014.2:2000SEP084439530H1 1245 1489
80 LG:245014.2:2000SEP087595283H 412 860
1
80 LG:245014,2:2000SEP085399133H1 613 821
80 LG:245014.2:2000SEP087695655) 652 1151
1
80 LG:245014,2:2000SEP083450361 R6 679 1143
80 LG:245014.2:2000SEP083450361 H 679 934
1
80 LG:245014.2:2000SEP082731452F6 692 1026
80 ~LG:245014.2:2000SEP082731452H 692 927
1
80 LG:245014.2:2000SEP08263691H1 705 1032
80 LG:245014,2:2000SEP085674201H1 717 953
80 LG:245014.2:2000SEP0&2645021 H 8 i 5 1063
1
~
80 LG:245014,2:2000SEP086452902H1 818 1267
80 LG:245014.2:2000SEP087619528H 927 1349
1
80 LG:245014,2:2000SEP08g895569 952 1113
80 LG:245014,2:2000SEP084797535H1 966 1248
80 LG:245014.2:2000SEP084164512H 973 1273
1
80 LG:245014,2:2000SEP08g 1128352 979 1212
80 LG:245014.2:2000SEP08g2003284 1060 1310
80 LG:245014,2:2000SEP086513260H1 1130 1619
80 LG:245014.2:2000SEP087695655H 1138 1780
1
80 LG:245014.2:2000SEP08g878573 1147 1466
80 LG:245014.2:2000SEP085948141 H 1154 1492
1
80 LG:245014.2:2000SEP082749018H 1176 1422
1
80 LG:245014.2:2000SEP083450361T6 1193 1751
i~-6
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
80 LG:245014.2:2000SEP082910268H 1210 1468
1
80 LG:245014.2;2000SEP084372565H1 1212 1510
80 LG;245014.2;2000SEP08133005H1 1217 1398
80 LG:245014.2:2000SEP0813300586 1217 1651
80 LG:245014.2:2000SEP086345408H 1222 1495
1
81 LG:170754.4:2000SEP086450115H1 177 241
81 LG;170754.4;2000SEP087621674H1 293 761
81 LG:170754.4:2000SEP081525016H 316 525
1
81 LG:170754.4;2000SEP08338566476 656 1080
81 LG:170754.4:2000SEP08g 1291209 716 1120
81 LG;170754.4;2000SEP086578227H1 857 1088
81 LG;170754.4:2000SEP087586739H1 6 433
81 LG:170754.4:2000SEP08737351 OH 1 476
1
81 LG:170754.4;2000SEP083288727H1 1 251
81 LG.170754.4:2000SEP087621674) 1 672
1
82 LG;988028.1:2000SEP085770980F8 1085 1641
82 LG:988028.1:2000SEP085770980H1 1073 1594
82 LG:988028.1:2000SEP086267543F8 1 657
82 LG;988028.1:2000SEP086568067F8 1 665
82 LG:988028.1;2000SEP086267543H1 1 536
82 LG:988028.1;2000SEP08626754378 158 699
82 LG:988028.1:2000SEP087765486) 501 1150
' 1
82 LG:988028.1:2000SEP087765486H1 1353 1941
82 LG;988028.1;2000SEP08g1303480 1368 1608
82 LG:988028.1:2000SEP08' 6355546F7 1473 1797
82 LG:988028.1;2000SEP086355546H 1473 1677
1
82 LG;988028.1;2000SEP086568067H1 1 62
82 LG:988028.1:2000SEP087330071H1 870 1307
82 LG:988028.1;2000SEP085625452H1 511 641
82 LG:988028.1:2000SEP087388455H 649 1075
1
83 LG:427997.6:2000SEP083751266F6 1 358
83 LG;427997.6:2000SEP083751266H1 1 296
83 LG:427997.b:2000SEP085376913H1 683 945
83 LG;427997.b;2000SEP082681128F6 695 1170
83 LG:427997.b;2000SEP082681128H1 696 978
83 LG:427997.b:2000SEP08191573686 731 1130
83 LG:427997.6:2000SEP08g850466 493 823
83 LG;427997.6;2000SEP082557738H1 650 890
83 LG:427997.b:2000SEP082560627H1 650 908
83 LG:427997.6;2000SEP083321818H1 104 350
83 LG;427997.6:2000SEP086351045H2 329 660
83 LG:427997.b:2000SEP083321818F6 103 497
83 LG:427997.b;2000SEP081915736H1 731 964
83 LG:427997.b:2000SEP085296714H 764 1029
1
83 LG:427997.b:2000SEP083934538H1 892 1190
83 LG;427997.b:2000SEP083934758H1 892 1185
83 LG:427997.6;2000SEP083934357H1 892 1190
83 LG:427997.b:2000SEP083934758F6 892 1393
147
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: 1D
83 LG:427997.b:2000SEP084177726H1 807 1094
83 LG;427997.b:2000SEP087059995H1 797 1258
83 LG:427997,6;2000SEP085946829H1 1037 1312
83 LG:427997.b;2000SEP086202890H1 1069 1601
a
83 LG:427997.6:2000SEP085947885H1 1093 1350
83 LG;427997.b;2000SEP083662984H1 999 1241
83 LG;427997.b;2000SEP083931985H1 893 1172
83 LG;427997.b:2000SEP083964084H 1 999 1281
83 LG;427997.b:2000SEP083672409H1 932 1159
83 LG;427997.b:2000SEP084717156H1 1264 1486
83 LG:427997.b;2000SEP086329373H1 1222 1746
83 LG:427997.6:2000SEP084174878H 1 1130 1402
83 LG;427997.6;2000SEP083792653H1 1211 1415
83 LG:427997.b;2000SEP08g2198304 1125 1210
83 LG;427997.b;2000SEP08096309H1 1094 1310
83 LG:427997.b;2000SEP083408296H1 1533 1778
83 LG;427997.6;2000SEP08317395976 1555 2141
83 LG:427997.b;2000SEP08229837486 1591 2043
83 LG;427997.b;2000SEP083173959H1 1309 1560
83 LG:427997.b:2000SEP081865168H1 1333 1576
83 LG;427997.b:2000SEP082273538H1 1473 1729
83 LG:427997.6;2000SEP083022474H1 1514 1799
83 LG:427997.6:2000SEP085287845H 1 1301 1423
83 LG;427997.6:2000SEP085290693H1 1301 1581
83 LG:427997.6;2000SEP083173959F6 1309 1852
83 LG;427997.6:2000SEP08g 1981676 1309 1621
83 LG:427997.6:2000SEP085391806H1 1269 1461
83 LG;427997.6:2000SEP08g4901915 1782 2188
83 LG:427997.6;2000SEP08162359176 1785 2143
83 LG;427997.6:2000SEP081853191 H 1672 1926
1
83 LG:427997.b;2000SEP08614199H1 1672 1899
83 LG;427997.b:2000SEP08168357376 1691 2141
83 LG:427997.b:2000SEP08375126676 1691 2153
83 LG;427997.6:2000SEP08332181876 1696 2151
83 LG;427997.6:2000SEP08229837476 1701 2148
83 LG:427997.b:2000SEP08~ 3021149H1 1724 2013
83 LG:427997.6;2000SEP08g2879046 1741 2191
83 LG;427997.6;2000SEP081853191 Tb 1747 2342
83 LG;427997.b;2000SEP08g2444573 1760 2187
83 LG:427997.b;2000SEP08g6656753 1761 2188
83 LG;427997.b:2000SEP084778510H1 1768 2046
83 LG:427997,6:2000SEP081361366H1 1624 1800
83 LG;427997.b;2000SEP081683573H1 1638 1873
83 LG:427997.b:2000SEP081683557F6 1638 2193
83 LG:427997.b:2000SEP081336611H1 1664 1907
83 LG;427997.6;2000SEP081853191 F6 1672 2184
83 LG:427997.b:2000SEP081632340H1 1671 1888
83 LG:427997.b:2000SEP08_1 683573F6 1638 2052
148
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
83 LG:427997.6:2000SEP082298374H 1591 1861
1
83 LG:427997,b:2000SEP081361272F1 1624 2182
83 LG;427997,6:2000SEP08223027H1 2029 2188
83 LG;427997.6:2000SEP08223027F1 2030 2188
83 LG;427997.6:2000SEP08g2541182 2072 2390
83 LG;427997,b;2000SEP08g3039868 2113 2191
83 LG:427997,6:2000SEP082086141 H1 1830 2093
83 LG:427997,b:2000SEP08423944278 1850 2260
83 LG;427997,6;2000SEP082408847H1 1848 2098
83 LG:427997,6:2000SEP08g5855887 1867 2189
83 LG;427997,b:2000SEP0822302781 2030 2188
83 LG;427997,b;2000SEP08g5858285 1867 2190
83 LG;427997.6:2000SEP083409241 Tb 1877 2333
83 LG:427997,b:2000SEP085669576H1 1897 2087
83 LG;427997.b;2000SEP083628624H1 1902 2217
83 LG;427997,6:2000SEP08g2969311 1904 2188
83 LG;427997,b:2000SEP08g3151378 1926 2192
83 LG:427997.b;2000SEP08g1980460 1961 2279
83 LG;427997,b:2000SEP08191573676 1784 2142
83 LG:427997.b:2000SEP08g3155476 1787 2174
83 LG;427997,b:2000SEP08g5392637 1792 2188
83 LG:427997.b:2000SEP08g6975043 1806 2189
83 LG;427997,b;2000SEP081623591 Fb 1819 2188
83 LG:427997.b;2000SEP081623591 H 1819 2040
1
83 LG:427997.6:2000SEP08g2198272 18274 2186
83 LG:427997.6:2000SEP0896051681 1825 2188
~
83 LG;427997.b:2000SEP0896038771 1825 2151
83 LG;427997.6;2000SEP08960516H1 1825 2126
84 LG:464206,1:2000SEP087613386J 244 326
1
84 LG:4b4206.1;2000SEP087613386H 326 527
1
84 LG:464206.1;2000SEP087581049H1 353 883
84 LG:464206.1:2000SEP086999751 R8 360 972
84 LG;464206.1;2000SEP087613178J1 235 527
84 LG;464206.1:2000SEP083976831H1 53 172
84 LG:464206.1:2000SEP087613178H 229 527
1
84 LG:4b4206.1:2000SEP086999751H1 35 325
84 LG:464206.1:2000SEP08619642478 1 223
84 LG;464206.1;2000SEP086999751 F8 33 309
85 LG:1400108,1;2000SEP08627139678 413 839
85 LG;1400108.1:2000SEP08358415276 546 1021
85 LG:1400108.1;2000SEP081541986H1 565 737
85 LG:1400108,1;2000SEP08g6041310 1 309
85 LG;1400108.1:2000SEP085405685H 154 390
1
85 LG:1400108,1:2000SEP0871 6l 67788 191 434
85 LG:1400108.1:2000SEP086517842H 708 1223
1
85 LG:1400108.1:2000SEP082537227H 234 501
1
85 LG:1400108.1:2000SEP082537227F6 234 821
85 LG:1400108.1:2000SEP08g5436494 346 733
149
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
85 LG:1400108.1;2000SEP087040568H 306 711
1
85 LG:1400108.1;2000SEP084456536H 587 788
1
85 LG:1400108.1:2000SEP084303918H1 299 475
86 LG:254531.1:2000SEP08211674586 1 429
86 LG;254531.1;2000SEP08421393776 179 561
86 LG;254531.1:2000SEP08398352476 288 650
86 LG:254531.1;2000SEP08g5513295 318 743
86 LG:254531.1;2000SEP082116745H 1 238
1
86 LG;254531.1;2000SEP084771743H1 613 753
86 LG;254531.1:2000SEP08477174376 833 899
86 LG:254531.1;2000SEP084771743F6 613 894
86 LG;254531.1;2000SEP08g5678401 420 739
86 LG;254531.1:2000SEP08g4082588 420 740
86 LG;254531.1:2000SEP08g4148835 409 ' 739
86 LG;254531.1:2000SEP08g5369564 327 752
87 LG;1101317.1:2000SEP084111645H1 2107 2376
87 LG:1101317.1:2000SEP081719261H1 2130 2340
87 LG;1101317.1:2000SEP08217174H1 2135 2326
87 LG:1101317.1;2000SEP08234490876 2149 2693
87 LG;1101317.1:2000SEP087356032H1 2156 2648
87 LG:1101317.1:2000SEP081861240H1 1990 2250
87 LG:1101317.1:2000SEP083094796H1 2026 2320
87 LG:1101317.1;2000SEP087216644H1 2079 2650
87 LG:1101317.1:2000SEP08g1961970 2087 2358
87 LG;1101317.1:2000SEP085888913H1 2089 2335
87 LG:1101317.1:2000SEP085884544H1 2089 2350
87 LG:1101317.1;2000SEP08802026H 1 2092 2315
87 LG:1101317.1:2000SEP087243661H1 2103 2462
87 LG;1101317.1:2000SEP08g6402048 481 941
87 LG:1101317.1:2000SEP08'g2064228 489 942
87 LG;1101317.1;2000SEP087401413H 568 1059
1
87 LG:1101317.1:2000SEP087114194H2 712 1212
87 LG:1101317.1;2000SEP0814346176 738 1212
87 LG;1101317.1:2000SEP084763709H 754 1022
1
87 LG:1101317.1;2000SEP08223371 R1 1820 2310
87 LG:1101317.1;2000SEP081861240F6 1990 2391
87 LG;1101317.1;2000SEP08229654H 1 1820 2032
87 LG:1101317.1:2000SEP08223371 H 1820 2049
1
87 LG:1101317.1;2000SEP085604126H1 1845 2063
87 LG;1101317.1;2000SEP081598553H1 1851 2059
87 LG:1101317.1:2000SEP083387636H1 1865 1980
87 LG:1101317.1:2000SEP08907585H1 1866 2020
87 LG:1101317.1:2000SEP0890758582 1866 2433
87 LG:1101317.1:2000SEP086874748H1 1915 2386
87 LG:1101317.1:2000SEP08g1955731 1921 2228
87 LG:1101317.1;2000SEP083696080H1 1977 2259
87 LG:1101317.1:2000SEP08g 1963935 1 474
87 LG;1101317,1:2000SEP086817012H 1 ' 361
_ _ ___ 1
_-_
150
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
87 LG:1101317.1:2000SEP086823629H1 34 600
87 LG:1101317.1:2000SEP086812964J1 38 681
87 LG:11 O 1317.1:2000SEP086812964H 138 666
1
87 LG:11 O 1317,1:2000SEP086535644H 234 797
1
87 LG:1101317,1:2000SEP08143461 H 321 756
1
87 LG:1101317.1:2000SEP08143461 R6 321 745
87 LG:1101317.1:2000SEP086310047H1 1461 1989
87 LG:1101317.1:2000SEP086823629J1 1486 1952
87 LG:1101317.1:2000SEP082439960H1 1494 ' 1692
87 LG:1101317.1:2000SEP081956487H1 1503 1759
87 LG:1101317.1:2000SEP081452925H1 1724 1986
87 LG:1101317.1:2000SEP081452925F1 1724 2203
87 LG:1101317.1:2000SEP083522982H1 1728 1956
~
87 LG:1101317.1:2000SEP08g2110490 1734 2149
87 LG:1101317.1:2000SEP08g 126,6352 1743 2126
87 LG:1101317.1:2000SEP086535272H1 1742 2239
87 LG:1101317.1:2000SEP084510157H1 1749 2005
87 LG:1101317,1:2000SEP082915753H1 1761 1960
87 LG:1101317,1:2000SEP084571543H1 1778 2020
87 LG:1101317.1:2000SEP085054191 H 1795 2065
1
87 LG:1101317.1:2000SEP087609456J1 1539 2035
87 LG:1101317.1:2000SEP086578896H1 1566 2057
87 LG:1101317.1:2000SEP086332835H1 1566 1733
87 LG:1101317.1:2000SEP086578895H1 1571 1938
87 LG:1101317,1:2000SEP086817012J1 1583 2180
87 LG:1101317,1:2000SEP08g2016947 1597 1932
87 LG:1101317.1:2000SEP083785356H1 1654 1946
87 LG:1101317.1:2000SEP086335980H1 1660 2239
87 LG:1101317,1:2000SEP082354693F6 1660 2193
87 LG:1101317.1:2000SEP082354693H1 1660 1884
87 LG:1101317.1:2000SEP081877179H1 1695 1968
87 LG:1101317.1:2000SEP081877179F6 1695 1984
87 LG:1101317,1:2000SEP081620310H1 1697 1938
87 LG:1101317,1:2000SEP08g2059298 2344 2734
87 LG:1101317.1:2000SEP08g3434517 2343 2731
87 LG:1101317.1:2000SEP08g3753098 2346 2731
87 LG:1101317.1:2000SEP08344433H1 2346 2574
87 LG:1101317,1:2000SEP081877179Th 2346 2688
87 LG:1101317.1:2000SEP085512710H1 2351 2570
87 ~ LG:1101317.1:2000SEP08g3701288 2376 2737
87 LG:1101317.1:2000SEP08g2583867 2378 2732
87 LG:1101317.1:2000SEP082607706H1 2379 2639
87 LG:1101317.1:2000SEP08g3741708 2381 2733
87 LG:1101317.1:2000SEP08g3229991 2384 2731
87 LG:1101317,1:2000SEP08g5036157 2390 2726
87 LG:1101317.1:2000SEP085055888H1 2399 2671
87 LG:1101317.1:2000SEP082354693T6 2415 2690
87 LG:1101317.1:2000SEP08g4296980 2419 2727
151
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
87 LG:1101317.1:2000SEP085297009H1 2447 2718
87 LG:1101317.1:2000SEP085296909H1 2447 2654
87 LG;1101317.1:2000SEP08643900H 1 2475 2718
87 LG:1101317.1:2000SEP0864390086 2475 2731
87 LG:1101317.1;2000SEP0864390076 2475 2693
87 LG:1101317.1:2000SEP08g6640298 2487 2731
87 LG;1101317.1;2000SEP082318777H1 2625 2722
87 LG;1101317.1:2000SEP087074835H1 2633 3063
87 LG:1101317.1:2000SEP08g3148127 2640 2734
87 LG;1101317.1:2000SEP08g2984877 2643 2739
87 LG:1101317.1;2000SEP08g712170 2650 2735
87 LG:1101317.1:2000SEP08g2909104 2663 2741
87 LG;1101317.1;2000SEP082955382H1 2671 2802
87 LG;1101317.1:2000SEP08g 1225713 2310 2730
87 LG:1101317.1:2000SEP08g3203907 2321 2737
87 LG:1101317.1:2000SEP086439019H1 2337 2682
87 LG:1101317.1;2000SEP08g3239341 2298 2732
87 LG;1101317.1;2000SEP08g2675052 2340 2738
87 LG:1101317.1:2000SEP08g4153003 2340 2731
87 LG;1101317.1:2000SEP082945971H1 2182 2482
87 LG;1101317.1;2000SEP082944561H1 2183 2485
87 LG:1101317.1;2000SEP083016776H1 2183 2482
87 LG:1101317.1:2000SEP086191566H1 2194 2502
87 LG;1101317.1;2000SEP086209904H1 2196 2501
87 LG:1101317.1:2000SEP084908028H1 2176 2419
87 LG:1101317.1:2000SEP086190886H1 2201 2376
87 LG:1101317.1:2000SEP08186124076 2252 2707
87 LG;1101317.1;2000SEP08223371 Fl 2252 2731
87 LG:11 O 1317.1;2000SEP08683952H 1 2285 2484
87 LG;1101317.1:2000SEP08164305176 2285 2684
87 LG:1101317.1;2000SEP08g4243434 2286 2734
87 LG:1101317.1:2000SEP083860584H1 2291 2524
87 LG:1101317.1:2000SEP084337054H1 2297 2549
87 LG:1101317.1;2000SEP087439030H1 1225 1748
87 LG;1101317.1:2000SEP081313614H1 1234 1402
87 LG:1101317.1:2000SEP085633301H1 1255 1413
87 LG:1101317.1:2000SEP087932553H1 1296 1903
87 LG:1101317.1:2000SEP086864228H1 1333 1854
87 LG:1101317.1:2000SEP087171990H1 1346 1904
87 LG:1101317.1;2000SEP086316428H1 1362 1642
87 LG:1101317.1:2000SEP082344908F6 1448 1999
87 LG:1101317.1:2000SEP082344908H1 1448 1719
87 LG:1101317.1;2000SEP086303664H1 1461 1748
87 LG:I 101317.1;2000SEP081643051 H1 896 1111
87 LG:1101317.1:2000SEP08g2069483 923 1308
87 LG:1101317.1;2000SEP087762822H 806 1197
1
87 LG;1101317.1;2000SEP087762822) 806 1197
1
87 LG:1101317.1:2000SEP087712440) 876 1486
1
1.52
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
87 LG;11 O 1317.1:2000SEP081643051 F6 896 1251
87 LG:1101317.1:2000SEP084352568H1 933 1149
87 LG:1101317.1:2000SEP086955572H1 1013 1577
87 LG:1101317.1:2000SEP086954828H1 1100 1529
87 LG:1101317.1:2000SEP087056589H1 1120 1604
88 LG:1074728.b:2000SEP083115529H1 18 316
88 LG;1074728.b:2000SEP08g 1847994 177 565
88 LG:1074728.b:2000SEP082740256H1 1 194
88 LG:1074728.6:2000SEP08g697772 7 228
88 LG;1074728.b:2000SEP08g763738 1 95
88 LG;1074728.b;2000SEP083276680H 1 200
1
88 LG;1074728.b;2000SEP086289128H2 70 327
88 LG:1074728.b;2000SEP08g763739 368 564
88 LG:1074728.b;2000SEP081351083H1 303 559
88 LG:1074728.b:2000SEP081634580Th 186 561
88 LG:1074728.6:2000SEP082622314H 228 415
1
88 LG:1074728.6:2000SEP08g2245965 455 565
88 LG:1074728.6:2000SEP08g 1846405 446 564
89 LG:1081 b84.1;2000SEP08g3675256 556 967
89 LG:1081684.1:2000SEP086772905) 356 958
1
89 LG;1081 b84.1:2000SEP082189954F6 594 950
89 LG;1081684.1;2000SEP086198434H1 485 940
89 LG:1081684.1;2000SEP083215765F6 107 722
89 LG:1081684.1:2000SEP086772905H 1 577
1
89 LG:1081684.1:2000SEP083324630H1 29 289
90 LG:1076520.1;2000SEP08g5100723 625 705
90 LG:107b520.1:2000SEP086753645H 488 960
1
90 LG:1076520.1;2000SEP087602495H 634 881
1
90 LG:1076520.1:2000SEP086866862H1 296 824
90 LG;1076520.1:2000SEP086752544) 1 467
1
90 LG:1076520.1:2000SEP08g 1476958 1 288
90 LG:1076520.1:2000SEP087390806H 1 255
1
90 LG:1076520.1:2000SEP08g4147917 604 710
90 LG:1076520.1;2000SEP08g5448756 604 667
90 LG:107b520.1:2000SEP08g5837902 604 710
90 LG:107b520.1:2000SEP08g5592223 604 710
90 LG;1076520.1:2000SEP08g5886003 604 710
90 LG:1076520.1:2000SEP08g 1479703 1 398
90 LG:1076520.1:2000SEP08g6703597 604 710
90 LG:1076520.1:2000SEP08g 1425839 96 380
90 LG:1076520.1:2000SEP08g4453645 604 700
90 LG:1076520.1:2000SEP08g3053258 604 700
90 LG:1076520.1:2000SEP08g5590354 604 710
90 LG:107b520.1:2000SEP08g4078684 604 710
90 LG:107b520.1:2000SEP08g 1481928 1 324
90 LG:107b520.1:2000SEP08g4189996 604 710
90 LG:1076520.1:2000SEP08g5811183 604 710
90 LG:1076520.1;2000SEP08g4395305 604 710
153
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Starf Stop
NO; ID
90 LG:1076520.1:2000SEP08g5633967 604 710
90 LG:1076520.1:2000SEP08g5755395 604 667
90 LG:1076520.1;2000SEP08g6975933 1 435
90 LG:1076520.1;2000SEP08g4690380 1 369
90 LG:1076520.1:2000SEP08g4194536 604 710
90 LG:1076520.1:2000SEP08g5933592 604 697
90 LG:1076520.1:2000SEP08g4450845 604 697
90 LG:107b520.1:2000SEP08g5232666 604 667
90 LG:107b520.1:2000SEP08g5544709 604 700
90 LG:1076520.1:2000SEP08g 14819.20 1 274
90 LG:1076520.1;2000SEP08675254488 1 369
90 LG;1076520.1:2000SEP08g4389567 604 710
91 LG;1079477.1;2000SEP082998325F6 148 570
91 LG:1079477.1:2000SEP08g5664125 340 649
91 LG;1079477.1:2000SEP082642788F6 336 624
91 LG:1079477.1:2000SEP08264278876 329 607
91 LG:1079477.1;2000SEP08299832576 141 606
91 LG:1079477.1;2000SEP087752481 H1 1 576
91 LG:1079477.1:2000SEP082642788H 336 552
1
91 LG:1079477.1:2000SEP08~ 150330676 190 598
91 LG;1079477.1;2000SEP082998325H 148 431
1
91 LG:I 079477.1;2000SEP08' g2898782 497 624
91 LG:1079477.1:2000SEP08g3858507 359 624
91 LG:1079477.1;2000SEP08838460H1 202 436
92 LG:10762b9.1;2000SEP086125643H 1 524
1
92 LG;1076269.1:2000SEP086128061 H 1 464
1
92 LG:1076269.1:2000SEP086128061 F8 1 649
92 LG:1076269.1:2000SEP086l 25641 1 634
F8
92 LG;1076269.1:2000SEP086125641 H 1 494
1
92 LG:1076269.1:2000SEP086125643F8 1 619
92 LG:10762b9.1:2000SEP086125641 T8 113 678
92 LG:10762b9.1:2000SEP08612564378 124 681
92 LG:107b2b9.1;2000SEP086128061 T8 358 684
92 LG;10762b9.1;2000SEP08g4510592 441 765
93 LG:1087195.1:2000SEP08g4510116 1046 1229
93 LG:1087195.1;2000SEP082633038F6 1090 1676
93 LG;1087195.1:2000SEP087632576H 1 598
1
93 LG:1087195.1:2000SEP086603749H 223 327
1
93 LG:1087195.1:2000SEP086405233H2 397 598
93 LG:1087195.1:2000SEP08g 1080813 421 617
93 LG:1087195.1:2000SEP08g988674 485 595
93 LG:1087195.1:2000SEP08g749322 485 761
93 LG:1087195.1:2000SEP086407533H 487 784
1
93 LG:1087195.1:2000SEP086164637H 542 1046
1
93 LG:1087195.1:2000SEP086405483H1 598 873
93 LG;1087195.1:2000SEP084145385F6 641 1146
93 LG:1087195.1:2000SEP084145385H 642 947
1
93 LG:1087195.1:2000SEP08_208_214276 704 1190
154
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6~ Template ID Component Start Stop
ID NO: ID
93 LG;1087195.1:2000SEP087632576J 710 ~ 1197
1
93 LG:1087195.1;2000SEP08182400376 731 1180
93 LG:1087195.1:2000SEP08g851810 753 1165
93 LG:1087195.1:2000SEP084825109H1 764 1016
93 LG:1087195.1:2000SEP08344746477 794 1076
93 LG:1087195.1;2000SEP08g2705361 804 1229
93 LG:1087195.1;2000SEP08g2069614 829 1236
93 LG:1087195.1:2000SEP084145486H 836 1112
1
93 LG;1087195.1:2000SEP08g3932054 839 1229
93 LG;1087195.1:2000SEP08g2590302 842 1229
93 LG;1087195.1;2000SEP08g3895320 847 1230
93 LG:1087195.1;2000SEP08g3895326 849 1230
93 LG:1087195.1:2000SEP084558427H1 857 1105
93 LG:1087195.1:2000SEP08g2913535 863 1196
93 LG;1087195.1;2000SEP08273125H 1 877 1102
93 LG:1087195.1;2000SEP08208221176 899 1190
93 LG:1087195.1:2000SEP082082211 H 906 1178
1
93 LG:1087195.1:2000SEP082082211 F6 906 1234
93 LG:1087 T 95.1:2000SEP086394752H 912 1191
1
93 LG:1087195.1;2000SEP08g 1226999 924 ~ 1229
93 LG:1087195.1:2000SEP08g 1501463 934 1229
93 LG:1087195.1:2000SEP086396710H1 998 1275
93 LG:1087195.1:2000SEP082633038H1 1090 1338
93 LG:1087195.1;2000SEP08g3069203 1158 1529
93 LG:1087195.1;2000SEP08g3253858 11 b5 1229
94 LG:002588.7;2000SEP08713465688 1 595
94 LG:002588.7;2000SEP08g1952446 129 461
94 LG;002588.7:2000SEP08g2191622 244 619
94 LG;002588.7;2000SEP085513362H1 389 624
94 LG:002588.7:2000SEP085513362F6 389 719
94 LG;002588.7:2000SEP08551336286 547 933
94 LG;002588.7;2000SEP087134656H1 1 278
95 LG:1079470.b:2000SEP08g675102 77 433
95 LG:1079470.b:2000SEP083806727H1 62 187
95 LG:T079470.b;2000SEP08g883656 57 427
95 LG:1079470.b:2000SEP08g706516 56 284
95 LG:1079470.b;2000SEP084572811H1 1 129
95 LG:1079470.b:2000SEP087328676H 17 458
1
95 LG:1079470.6:2000SEP082718147H1 485 722
95 LG;1079470.b;2000SEP087747301 H1 530 1045
~95 LG:1079470.6;2000SEP084175267H1 584 869
95 LG:1079470.6:2000SEP083600095H 589 845
1
95 LG:1079470.b;2000SEP084219975H1 603 895
95 LG:1079470.6;2000SEP08g1439462 634 853
95 LG:1079470.6;2000SEP083948450F7 655 1174
95 LG;1079470.b:2000SEP083948450F8 656 1145
95 LG:1079470.6:2000SEP083946142H1 655 835
95 LG:1079470.b;2000SEP083948450H1 655 839
_ ____
155
CA 02419943 2003-02-20
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TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
95 LG:1079470,b:2000SEP083946142F8 658 1195
95 LG:1079470,6:2000SEP0863894286 662 1217
95 LG:1079470.6:2000SEP082550648F6 662 1027
95 LG:1079470,6:2000SEP08638942H1 662 917
95 LG:1079470.b:2000SEP082550648H 662 904
1
95 LG:1079470.b:2000SEP085219834H2 687 921
95 LG:1079470.6:2000SEP0854374886 480 1034
95 LG:1079470.b:2000SEP085546359H1 484 677
95 LG:1079470,b:2000SEP085219834F7 690 1243
95 LG:1079470,6:2000SEP082318794H1 715 860
95 LG:1079470.6:2000SEP085202357H1 763 985
95 LG:1079470,6:2000SEP083466746H 764 1006
1
95 LG:1079470,6:2000SEP08g1302678 799 1071
95 LG:1079470.6;2000SEP082050882H1 825 1077
95 LG:1079470,6:2000SEP082733580H 871 1094
1
95 LG:1079470,6:2000SEP084919329H1 893 1119
95 LG:1079470.b:2000SEP086167338H1 947 1025
95 LG:1079470,b:2000SEP086060464F8 411 1027
95 LG:1079470,6:2000SEP085996080F8 411 937
95 LG:1079470.b:2000SEP081451495F6 448 1072
95 LG:1079470.6:2000SEP081451495F1 448 917
95 LG:1079470,6:2000SEP081451495H1 448 695
95 LG:1079470.6:2000SEP08543748H 1 480 708
95 LG:1079470.b:2000SEP0854374881 480 865
95 LG:1079470,6:2000SEP085761741H1 299 577
95 LG:1079470.6:2000SEP084717311 H 302 535
1
95 LG:1079470.6:2000SEP08299i334Hi 80 419
95 LG:1079470,6:2000SEP08gb197278 293 750
95 LG:1079470,6:2000SEP085757130F8 296 883
95 LG:1079470.b:2000SEP085757130H1 296 576
95 LG:1079470.6:2000SEP085996080H 411 684
1
95 LG:1079470.6:2000SEP086541925H1 325 851
95 LG:1079470,6:2000SEP08g965206 353 699
95 LG:1079470,b:2000SEP084717311 F8 325 823
95 LG:1079470.b:2000SEP086779294H 372 944
1
95 LG:1079470,6:2000SEP082266214H1 382 648
95 LG:1079470.6:2000SEP085996080F7 41 T 9i9
95 LG:1079470,6:2000SEP085992238H1 411 700
96 LG:345705,3:2000SEP086866333H1 1 508
96 LG:345705,3:2000SEP086743970H 78 400
1
96 LG:345705.3:2000SEP085903130H1 1 270
9b LG:345705.3:2000SEP083134791 H 209 481
1
97 LG:1083b54,1:2000SEP08296137376 2740 2962
97 LG:1083654.1:2000SEP08243195H 1 2742 2862
97 LG:1083654.1:2000SEP082961373F6 2747 2997
97 LG:1083654.1:2000SEP082961373H 2749 2877
1
97 LG: 7083654, i 5308958H 2786 3006
:2000SEP08 1
97 LG:1083654.1:2000SEP081251913H 2867 3008
1
156
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
97 LG;1083b54.1;2000SEP081251913F6 2873 2951
97 LG:1083654.1:2000SEP08285359176 2884 3294
97 LG:1083654.1;2000SEP08171277876 2965 3407
97 LG:1083b54.1:2000SEP088897183 3094 3370
97 LG:1083654.1:2000SEP087932016H1 3113 3732
97 LG:1083654.1:2000SEP0882057056 2249 2605
97 LG:1083b54.1;2000SEP08655886578 2319 2892
97 LG:1083b54.1:2000SEP081712778H1 2320 2534
97 LG:1083654.1:2000SEP081712778F6 2320 2857
97 LG:1083654.1:2000SEP084787383H1 2530 2781
97 LG:1083b54.1;2000SEP0884629623 2555 3002
97 LG:1083654.1;2000SEP0885512397 2563 3003
97 LG;1083654.1:2000SEP0885661867 2578 3033
97 LG:1083654.1:2000SEP085445604H1 2591 2832
97 LG:1083b54.1:2000SEP0885511864 2593 3003
97 LG;1083654.1:2000SEP0882070577 2641 3010
97 LG:1083b54.1:2000SEP0884334177 2644 3011
97 LG:1083b54.1;2000SEP0882715986 2678 3002
97 LG:1083654.1:2000SEP086513330H1 2735 3027
97 LG:1083b54.1:2000SEP087664249H 250 808
1
97 LG:1083b54.1;2000SEP087011821 H1 430 863
97 LG:1083654.1;2000SEP0886438877 427 839
97 LG:1,083654.1:2000SEP0883679253 430 886
97 LG:1083b54.1:2000SEP0885231632 430 849
97 LG:1083b54.1;2000SEP0883665440 430 880
97 LG;1083654.1:2000SEP0885554573 430 894
97 LG:1083654.1;2000SEP0886040151 433 870
97 LG:1083654.1;2000SEP08g 1242884 435 770
97 LG:1083654.1:2000SEP0884190333 436 814
97 LG;1083654.1:2000SEP08569484176 481 910
97 LG:1083654.1;2000SEP0882240560 494 973
97 LG:1083654.1;2000SEP08g 1367813 558 1328
97 LG:1083654.1:2000SEP085695241 T8 635 1078
97 LG:1083b54.1;2000SEP08g 1367787 750 1356
97 LG:1083654.1:2000SEP084031856H 991 1223
1
97 LG;1083654.1:2000SEP0886986275 1015 1569
97 LG:1083654.1:2000SEP086558865F8 1261 1866
97 LG:1083654.1:2000SEP086558865H1 1261 1764
97 LG;1083654.1:2000SEP087664249) 1363 1917
1
97 LG:1083b54.1;2000SEP085695241F9 1382 1960
97 LG:1083654.1:2000SEP084249967H1 1401 1654
97 LG:1083b54.1:2000SEP084798922H1 1552 1795
97 LG:1083b54.1;2000SEP082853591F6 1617 1985
97 LG:1083b54.1:2000SEP082853591H1 1617 1703
97 LG;1083b54.1:2000SEP085694841F6 1665 1956
97 LG;1083b54.1;2000SEP081962583H 1668 1933
1
97 LG:1083654.1:2000SEP086843753F8 1691 2211
97 LG:1083654.1:2000SEP086843753H1 1691 2271
157
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
97 LG:1083b54.1:2000SEP085695241H1 1737 1960
97 LG:1083654.1;2000SEP084245847H1 1748 2009
97 LG;1083654.1:2000SEP084245325H1 1749 2014
97 LG:1083654.1:2000SEP084245361H1 1749 2013
97 LG:1083654.1:2000SEP083515501H1 1837 2080
97 LG:1083654.1:2000SEP082551309H1 2173 2418
97 LG:1083654.1:2000SEP087169394H 1 119
1
97 LG:1083b54.1:2000SEP08377885978 1 328
97 LG;1083b54.1:2000SEP08377885979 1 362
97 LG:1083b54.1.:2000SEP083778859F8 1 486
97 LG;1083b54.1:2000SEP083778859H 2 309
1
97 LG:1083654.1;2000SEP083778859F9 1 489
98 LG:198782.3:2000SEP083733962H1 6 299
98 LG;198782.3:2000SEP08g6946984 33 479
98 LG:198782.3:2000SEP081993570H 36 246
1
98 LG;198782.3:2000SEP081993470H 36 294
1
98 LG:198782.3:2000SEP081993570F6 36 514
98 LG:198782.3:2000SEP087727533)1 42 271
98 LG:198782.3:2000SEP087727533H1 57 275
98 LG:198782.3;2000SEP083232864H1 72 349
98 LG:198782.3:2000SEP08g2575182 80 444
98 LG;198782.3;2000SEP085375624H1 114 358
98 LG;198782.3:2000SEP085605969H1 181 450
98 LG;198782.3:2000SEP08g4458155 311 756
98 LG:198782.3:2000SER08g 1933416 316 808
98 LG:198782.3:2000SEP08g1924157 365 720
98 LG;198782.3:2000SEP084023516H1 383 688
98 LG:198782.3:2000SEP084165481 H 384 669
1
98 LG:198782.3;2000SEP084172681H1 383 645
98 LG;198782.3:2000SEP08g2219275 389 791
98 LG:198782.3:2000SEP087184257H1 456 1030
98 LG:198782.3:2000SEP08g 1526024 575 1045
98 LG;198782.3:2000SEP08g 1266311 625 888
98 LG;198782.3:2000SEP08g723408 672 951
98 LG:198782.3;2000SEP08g3070857 730 1203
98 LG:198782.3;2000SEP08g1933415 730 1187
98 LG:198782.3:2000SEP087213803H1 758 1175
98 LG;198782.3:2000SEP082433334H 769 982
1
98 LG:198782.3:2000SEP08g5854526 775 1200
98 LG;198782.3:2000SEP08g3784943 779 1203
98 LG;198782.3:2000SEP08g 1923460 785 1195
98 LG:198782.3:2000SEP084270541 H 811 1061
1
98 LG:198782.3:2000SEP08g 1525917 823 1200
98 LG:198782.3:2000SEP087090529H1 828 1374
98 LG;198782.3:2000SEP08g724366 842 1194
98 LG:198782.3:2000SEP08g5233108 862 ~ 1194
98 LG:198782.3:2000SEP08g2357872 906 1193
98 LG:198782.3:2000SEP08g2000179 1932 2187
_
158
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
98 LG:198782.3:2000SEP08g2000178 1932 2136
98 LG;198782.3;2000SEP086782233H 1820 2357
1
98 LG:198782.3;2000SEP087740905J1 1943 2550
98 LG:198782.3;2000SEP08g2715455 1696 2034
98 LG;198782.3;2000SEP083094122F6 1710 2122
98 LG;198782.3;2000SEP083094122H1 1710 1980
98 LG;198782.3;2000SEP086943391 H1 1759 2247
98 LG;198782.3;2000SEP087743465H1 1324 1941
98 LG:198782.3;2000SEP087740905H1 1566 ' 2180
98 LG:198782.3:2000SEP083069437F6 1148 1287
98 LG:198782.3;2000SEP083069437H1 1148 1418
98 LG:198782.3;2000SEP087042063H1 1207 1752
98 LG:198782.3:2000SEP08g1481929 1221 1671
98 LG;198782.3;2000SEP083382159H1 1 249
98 LG;198782.3:2000SEP083349345H1 1 218
98 LG;198782.3:2000SEP08g4435439 1 468
98 LG;198782.3:2000SEP086723501 H 2086 2517
1
98 LG;198782.3:2000SEP087727032H 2138 2554
1
98 LG:198782.3:2000SEP087591969H1 2241 2667
98 LG:198782.3:2000SEP086782233) 2352 2979
1
98 LG;198782.3:2000SEP08171482H 1 2396 2606
98 LG:198782.3:2000SEP08g2779400 913 1197
99 LG;981076.2;2000SEP087169509H1 1 476
99 LG;981076.2:2000SEP087409370H1 2 577
99 LG:981076.2;2000SEP086768733H1 57 291
99 LG:981076.2:2000SEP083176620H 65 319
1
99 LG:981076.2:2000SEP083176620F6 65 550
99 LG:981076.2;2000SEP084613367H1 71 325
99 LG;981076.2;2000SEP083082646H1 122 414
99 LG:981076.2:2000SEP083504115H1 161 476
99 LG:981076.2;2000SEP08g5178468 189 666
99 LG;981076.2:2000SEP08259700H1 1 277
99 LG;981076.2:2000SEP08351753386 331 652
99 LG:981076.2:2000SEP083517533H1 331 620
100 LG:212023.3:2000SEP081438825H1 226 479
100 LG;212023.3:2000SEP081438827H1 226 477
100 LG;212023.3:2000SEP085054201H1 234 536
100 LG:212023.3;2000SEP085054201 F6 239 743
100 LG:212023.3:2000SEP083699764H1 255 442
100 LG:212023.3:2000SEP084871594H1 276 522
100 LG:212023.3:2000SEP08g3215183 314 754
100 LG;212023.3:2000SEP08g2703431 326 761
100 LG:212023.3;2000SEP085654058H1 471 844
100 LG:212023.3:2000SEP082988962H 478 76b
1
100 LG;212023.3;2000SEP081387482H1 483 712
100 LG:212023.3;2000SEP08g2046330 598 933
100 LG:212023.3:2000SEP08068454H 1 656 808
100 LG;212023.3:2000SEP085654801 H1 693 981
159
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID ' Component Start Stop
NO; ID
100 LG:212023.3:2000SEP08g2106581 852 1214
100 LG:212023.3:2000SEP08594131476 994 11 b8
100 LG;212023.3:2000SEP085941314H1 993 1215
100 LG;212023.3:2000SEP081925734H1 1106 1333
100 LG:212023.3:2000SEP08192573476 1106 1316
100 LG:212023.3:2000SEP08192573486 1106 1316
100 LG:212023.3:2000SEP081438827F6 226 653
100 LG:212023.3:2000SEP08614697H1 1 233
100 LG:212023.3:2000SEP081284208F6 70 504
100. LG;212023.3:2000SEP081284208H1 70 255
100 LG:212023.3:2000SEP084828893H 1 115 341
100 LG;212023.3:2000SEP084873560H1 202 426
100 LG;212023.3:2000SEP084872742H1 218 488
1 O1 LG:977929.3:2000SEP086480392H 1 1 496
1 O1 LG:977929.3:2000SEP086480523H 1 1 479
1 O1 LG:977929.3:2000SEP08g 1974709 1 227
101 LG:977929.3:2000SEP08861670H1 8 274
101 LG;977929.3:2000SEP08g1988875 15 300
101 LG;977929.3;2000SEP083350527H1 62 153
102 LG;201936.6:2000SEP085089364F6 1 442
102 LG:201936.6:2000SEP085089364H 1 1 254
102 LG;201936.6;2000SEP085047236H1 283 536
102 LG:201936.6:2000SEP08649419489 413 987
102 LG:201936.6:2000SEP083590431 H ~ 520 836
1
102 LG;20193b.6:2000SEP085562609H1 585 804
102 LG:201936.6:2000SEP085562609F6 585 1000
102 LG:201936.b:2000SEP085137081 H 688 973
1
103 LG:205b42.1:2000SEP081787609H1 . 675. 911
103 LG;205642.1;2000SEP08631267678 866 1296
103 LG:205642.1:2000SEP08627470978 881 1354
103 LG:205b42.1:2000SEP08355515276 886 1382
103 LG:205642.1:2000SEP084753183H1 1294 1430
103 LG;205b42.1:2000SEP082120129H1 1 238
103 LG:205b42.1:2000SEP086312676H1 151 686
103 LG;205b42.1;2000SEP086273658F8 407 1073
103 LG;205642.1:2000SEP086274709H2 408 526
103 LG:205642.1:2000SEP083555152F6 497 924
103 LG;205b42.1:2000SEP083555152H1 497 782
104 LG:339653.6:2000SEP086038578H 1 7 301
104 LG;339653.6:2000SEP085957411H1 1 285
104 LG:339653.6:2000SEP086040578H 1 7 319
105 LG:978587.4:2000SEP08259209576 1 318
105 LG:978587.4;2000SEP08g6075351 143 601
105 LG:978587.4:2000SEP085000079H2 264 525
105 LG;978587.4:2000SEP084666688H1 328 521
105 LG:978587.4:2000SEP084666688F6 328 620
105 LG:978587.4:2000SEP084048832H 1 402 701
105 LG:978587.4:2000SEP08g2103012 485 712
160
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
106 LG;216848.17:2000SEP086477749H1 1 479
106 LG;216848,17:2000SEP087275903H2 89 475
107 LG:219502.1:2000SEP087343478H1 628 1213
107 LG:219502.1:2000SEP087587604H 653 828
1
107 LG:219502,1:2000SEP08g2114615 1 240
107 LG:219502,1:2000SEP08g2241493 1 337
107 LG:219502,1;2000SEP08288443376 1 303
107 LG:219502.1:2000SEP08g2112758 23 205
'
107 LG:219502,1:2000SEP08g2166952 76 388
107 LG:219502.1:2000SEP08g21 67050 264 727
107 LG;219502,1:2000SEP08g4186677 268 715
107 LG:219502,1:2000SEP08g6035041 268 602
107 LG:219502.1:2000SEP08g4896038 275 728
107 LG:219502,1:2000SEP087170772H 531 797
1
107 LG:219502,1:2000SEP086608778H 621 1133
1
108 LI;334211,1;2000SEP086096314F8 1 488
108 LI;334211.1:2000SEP084374110F6 140 448
108 LI:334211.1:2000SEP08437411 OH 140 421
1
108 LI;334211.1:2000SEP086096314H1 160 488
108 LI:334211.1:2000SEP087758978H 259 848
1
108 LI:334211,1:2000SEP086018226H 907 1301
1
108 LI;334211.1:2000SEP083119892F6 435 909
108 LI;334211,1;2000SEP083119892H1 435 697
108 LI;334211.1:2000SEP08311989276 811 1357
108 LI;334211.1;2000SEP087758978J1 824 1307
108 LI:334211.1:2000SEP086019811 H 833 1252
1
108 LI;334211,1:2000SEP083389338H 1109 1307
1
108 LI;334211,1:2000SEP088043131 H 1121 1330
1
109 LI:231024,2;2000SEP081784942H1 959 1191
109 LI:231024.2:2000SEP081618756F6 447 855
109 LI;231024.2:2000SEP081618504H1 447 671
109 LI;231024,2:2000SEP081618749H1 447 649
109 LI;231024.2:2000SEP081686804H1 450 670
109 LI:231024.2:2000SEP081785019H 450 698
1
109 LI:231024,2;2000SEP08g2835145 474 737
109 LI:231024.2:2000SEP08g6451273 1052 1414
109 LI;231024,2:2000SEP08g3331178 1077 1414
109 LI;231024.2:2000SEP08g1785426 748 1190
109 LI:231024,2:2000SEP082430567H1 755 1009
109 LI:231024,2:2000SEP084291692H1 788 883
109 LI;231024,2:2000SEP084291592H1 790 944
109 LI:231024,2:2000SEP084125405H1 874 1150
109 LI:231024.2:2000SEP084713559H1 499 750
109 LI;231024.2;2000SEP082214403F6 627 1061
109 LI:231024.2:2000SEP082214403H 627 876
1
109 LI;231024,2:2000SEP082212765H1 627 883
109 LI:231024.2:2000SEP085515783H1 703 934
109 LI:231024.2;2000SEP084290144H1 738 998
161
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
109 LI:231024.2:2000SEP082078150H 1 742 1004
109 LI;231024.2;2000SEP08g3092119 932 1417
109 LI:231024.2:2000SEP086725205H1 1 614
109 L1;231024.2:2000SEP08654199178 6b 662
109 LI;231024.2:2000SEP085964b4379 122 634
109 LI:231024.2;2000SEP08g2783200 342 848
109 LI;231024.2:2000SEP08g272b574 949 11 b9
109 Li:231024.2:2000SEP087684929H1 951 1478
109 LI:231024.2:2000SEP087683622H 1 951 1456
109 LI;231024.2:2000SEP087b82580H1 951 1457
109 LI:231024.2;2000SEP086713531H1 1171 1539
109 L1:231024.2:2000SEP08g55411 b9 1221 1545
109 LI:231024.2:2000SEP08g2958236 1228 1416
109 LI:231024.2;2000SEP08161875676 1249 1843
109 LI;231024.2:2000SEP087618177)1 1631 2103
109 LI;231024.2:2000SEP0819435b5H1 1700 1879
109 L1;231024.2:2000SEP0841451 b8H 829 1121
1
110 LI;228425.5:2000SEP088018076)1 184 780
110 LI:228425.5:2000SEP087589727H1 157 769
110 LI;228425.5;2000SEP087601932H1 425 b92
110 LI;228425.5:2000SEP087601924H1 502 692
110 LI;228425.5:2000SEP087601932)1 425 690
110 LI;228425.5;2000SEP087611327)1 22 658
110 LI:228425.5:2000SEP087743111H1 31 642
110 LI;228425.5:2000SEP088036091 J 123 643
1
110 LI:228425.5;2000SEP087601924)1 452 607
110 L1:228425.5:2000SEP088025995)2 27 575
110 LI:228425.5:2000SEP087627bO6H 1 1 518
110 LI:228425.5:2000SEP087750637) 1 158 419
110 LI;228425.5;2000SEP087983232H1 268 832
110 LI:228425.5:2000SEP0870942464V1 435 1030
110 L1:228425.5:2000SEP088018642) 1 353 989
110 LI:228425.5:2000SEP087667b52H1 261 836
111 LI:034493.1:2000SEP087016835H1 1 585
111 LI:034493.1;2000SEP08525053H1 35 267
111 LI:034493.1:2000SEP08g1b177b0 129 485
111 LI;034493.1:2000SEP087994078H1 474 996
112 LI;336218.1:2000SEP083154167H1 14 284
112 LI:336218.1:2000SEP085407028H1 14 216
112 LI:336218.1;2000SEP083267888H1 14 250
112 LI:336218.1:2000SEP085715861 H 31 501
1
112 LI;33b218.1:2000SEP082475067F6 41 400
112 LV:336218.1:2000SEP082475067H1 41 243
112 LI:336218.1:2000SEP083674722H 1 142 432
112 LI:33b218.1;2000SEP0829b4579H1 1b9 445
112 LI:33b218.1:2000SEP0860136174D1 241 424
112 LI;336218.1:2000SEP082475067Th 335 703
112 LI;336218.1;2000SEP087183496H1 8 512
162
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO. ID
112 LI:336218.1:2000SEP082840687F6 14 474
112 LI:33b218.1;2000SEP082840687H1 14 274
112 LI:336218.1:2000SEP087229614H1 1 266
112 LI:33b218.1:2000SEP085270428H1 1 185
112 LI:336218.1:2000SEP083233583H1 4 282
112 LI:336218.1:2000SEP087591929H 1 4 539
112 LI;336218.1:2000SEP083471708H1 407 595
113 LI:235891.3:2000SEP084893031 H2 1 282
113 LI:23~891.3;2000SEP0831169i4H1 22 323
113 . LI:235891.3:2000SEP0860268406D1 157 747
114 LI;344094.1;2000SEP08g667576 1 344
114 LI;344094.1:2000SEP08g697637 68 384
114 LI:344094.1;2000SEP08g1275867 105 547
114 LI:344094.1:2000SEP08g655556 207 516
114 LI:344094.1:2000SEP08g813917 237 594
114 LI:344094.1;2000SEP08g900307 237 547
114 LI:344094.1:2000SEP08g878170 237 635
114 LI:344094.1;2000SEP082922762F6 351 821
114 LI:344094.1:2000SEP082922762H1 351 425
114 LI:344094.1;2000SEP08g672909 359 546
114 LI:344094.1:2000SEP08g677456 359 543
114 LI:344094.1:2000SEP085492156H 1 421 654
114 LI:344094.1;2000SEP08g 1068390 487 743
114 LI:344094.1;2000SEP08g1043520 548 910
115 LI:399945.2;2000SEP08g5527625 356 685
115 LI:399945.2:2000SEP08g5236017 121 574
115 LI;399945.2;2000SEP08g47351 b0 144 574
i i 5 Li;399945.2:2000SEP08g57421 b8 131 574
115 LI:399945.2;2000SEP08g3932316 149 573
115 LI:399945.2:2000SEP08g3108874 211 571
115 LI:399945.2:2000SEP08~ g5594172 110 571
115 LI:399945.2;2000SEP08g5878951 129 571
115 LI:399945.2:2000SEP08g5054566 115 571
115 LI;399945.2:2000SEP08g4687041 128 571
115 LI:399945.2:2000SEP08g4393431 142 570
115 LI:399945.2;2000SEP08g4650676 221 569
115 LI:399945.2:2000SEP08g5769276 115 568
115 LI:399945.2;2000SEP08g6710022 113 568
115 LI:399945.2:2000SEP0885235149 102 568
115 LI:399945.2;2000SEP0883869794 95 568
115 LI:399945.2:2000SEP0887317560 283 568
115 LI;399945.2;2000SEP0882616056 271 560
115 LI:399945.2:2000SEP0884988831 417 521
115 LI:399945.2:2000SEP0885339565 1 474
115 LI:399945.2:2000SEP084207047H1 146 419
11 b LI:051849.1:2000SEP081468364F6 1 273
116 LI:051849.1:2000SEP081468364H1 1 140
116 LI;051849.1:2000SEP081468364T6 76 594
-
__ _ 16j
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
117 LI:238379.3:2000SEP0860220769D1 423 716
117 LI:238379.3:2000SEP084140076H1 187 484
117 LI:238379.3;2000SEP087203996H1 1 531
118 LI:352190.8:2000SEP087462355H 1 1 561
118 LI:352190.8:2000SEP082728751H1 99 359
118 LI:352190.8;2000SEP082728751 F6 99 610
118 LI;352190.8:2000SEP0870608138V1 476 732
118 LI:352190.8;2000SEP0870605012V1 473 1021
118 LI;352190.8:2000SEP0871485659V1 599 1205
119 LI:432120.1:2000SEP08g7375879 385 847
119 LI;432120.1:2000SEP08g6476525 387 846
119 LI:432120.1;2000SEP08g7281497 577 844
119 LI:432120.1:2000SEP08g4078434 408 842
119 LI:432120.1:2000SEP08g5755093 389 839
1 i 9 LI;432120.1:2000SEP086244543H 1 ~ 1 5b0
119 LI:432120.1;2000SEP086248154H 1 1 540
119 LI:432120.1:2000SEP086243676H 1 1 506
120 LI;0554b1.1;2000SEP084172005F6 752 1261
120 LI;055461.1:2000SEP084054692H1 752 1031
i 20 LI:0554b 1.1:2000SEP087057 i 894V 755 1327
1
120 LI;055461.1:2000SEP0870570215V1 812 1372
120 LI;055461.1:2000SEP0870571158V1 837 1341
120 LI:055461.1:2000SEP0870571350V1 861 1452
120 LI:0554b1.1;2000SEP0870569978V1 880 1517
120 LI;055461.1;2000SEP0870568295V1 879 1469
120 LI:055461.1;2000SEP0870564777V1 881 1289
120 LI:0554b1.1;2000SEP0870564128V1 886 1255
120 LI:055461.1:2000SEP0870563524V 887 1255
1
120 LI;0554b1.1:2000SEP0870567973V1 906 1369
120 LI:055461.1:2000SEP0870568827V1 909 1318
120 LI;055461.1:2000SEP0870571280V1 921 1479
120 LI:055461.1:2000SEP0870563885V1 914 1064
120 LI:055461. 7 :2000SEP0870572315V1 921 1478
120 LI:0554b1.1;2000SEP0870567979V1 947 1516
120 LI:055461.1;2000SEP082407240Th 1026 1509
120 LI;055461.1;2000SEP0870568955V1 1024 1627
120 LI:055461.1:2000SEP0870566789V1 1058 1324
1 20 LI:055461.1:2000SEP0870568622V 1065 1624
1
120 LI:055461.1:2000SEP0870570905V1 1090 1686
120 LI:0554b1.1;2000SEP0870570764V1 1089 1638
120 LI:0554b1.1:2000SEP08g6198775 1125 1525
120 LI:0554b1.1:2000SEP0870568160V1 1145 1627
120 LI:0554b1.1:2000SEP08g3118198 1161 1525
120 LI:0554b1.1;2000SEP0870568672V1 1183 1643
120 LI:055461.1:2000SEP0870568087V1 1205 1624
120 LI:055461.1:2000SEP0870568736V 1214 1628
1
120 LI:055461.1;2000SEP0870571754V1 1233 1627
120 LI:0554b1.1;2000SEP0870569000V1 1272 1648
~
164
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; iD
120 LI;055461.1:2000SEP081600869H1 1279 1527
120 LI;055461.1:2000SEP087974268H1 1 478
120 LI:0554b1.1:2000SEP084592590F8 13 578
120 LI;055461.1:2000SEP082457736F6 2b 480
120 LI;055461.1:2000SEP082457736H1 26 154
120 LI:055461.1:2000SEP084194633H1 95 407
120 LI;055461.1;2000SEP084194633F7 99 684
i 20 LI:055461.1:2000SEP082073295H 1 i 28 383
120 LI;055461.1;2000SEP082073295F6 128 484
120 LI;0554b1.1:2000SEP082895870H1 183 468
120 LI:055461.1;2000SEP08491179H 1 231 494
120 LI:0554b1.1:2000SEP081400739H1 292 535
120 LI;055461.1:2000SEP082286216H1 420 603
120 LI:055461.1:2000SEP08228621 6R6 420 815
120 LI;055461.1:2000SEP0870563652V1 428 619
120 LI:0554b1.1:2000SEP084670223H1 434 687
120 LI;055461.1:2000SEP0870571953V1 446 911
120 LI:055461.1:2000SEP0870571173V1 523 1020
120 LI:055461.1:2000SEP0870570848V1 530 1126
120 LI:055461.1:2000SEP0870570874V1 568 1142
120 LI;055461.1;2000SEP0870568559V1 571 1162
120 LI:055461.1:2000SEP0870568000V1 583 1137
120 LI:0554b1.1;2000SEP0870570860V1 601 911
120 LI:0554b1.1:2000SEP0870571070V1 657 ~ 1178
120 LI:055461.1:2000SEP0870569626V1 649 1262
120 LI:0554b1.1:2000SEP0870570457V1 658 1315
120 LI:055461.1:2000SEP087091663H 1 722 1072
120 LI:055461.1:2000SEP084172005H1 751 1021
120 LI;055461.1:2000SEP08g4329308 1303 1529
120 LI:055461.1;2000SEP0870569270V1 1315 1624
120 LI:055461.1:2000SEP082073295Th 1420 1624
120 LI;0554b1.1;2000SEP0870562650V1 1442 1644
120 LI:055461.1:2000SEP0870567951 V1 1457 1627
120 LI;055461.1:2000SEP0870568561V1 1511 1641
121 LI:197433.5:2000SEP08624501 OH 1 85
1
121 LI:197433.5;2000SEP084111192H1 2 292
121 LI:197433.5:2000SEP0870752461V1 9 511
121 LI:197433.5:2000SEP082923165F6 9 504
121 LI;197433.5:2000SEP0870756635V1 9 607
121 LI:197433.5:2000SEP0870751919V 9 514
1
121 LI:197433.5;2000SEP0870752173V1 9 481
121 LI:197433.5:2000SEP0870755802V 9 582
1
121 LI:197433.5:2000SEP082923165H1 9 296
121 LI:197433.5:2000SEP08534459H 1 14 244
121 LI:197433.5:2000SEP087075bObOVI 236 788
121 LI:197433.5:2000SEP0870747907V1 252 632
121 LI:197433.5:2000SEP0870754739V1 254 788
121 LI:197433.5:2000SEP083715708H 1 258 367
165
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Starf Stop
NO; ID
121 LI:197433.5:2000SEP0870772671 V1 267 688
121 LI:197433.5:2000SEP0870756945V1 267 813
121 LI:197433.5:2000SEP0870756464V1 266 788
121 LI:197433.5;2000SEP0870756300V1 266 788
121 LI:197433.5:2000SEP0870754898V 287 645
1
121 LI:197433.5:2000SEP0886087492 320 782
i2i LI:197433.5;2000SEP0883756770 361 783
121 LI;197433.5;2000SEP0883214623 364 784
121 LI:197433.5;2000SEP081454194Th 369 746
121 LI:197433,5:2000SEP0885176421 370 779
121 LI:197433.5:2000SEP0870752177V1 371 788
121 LI: i 97433.5:2000SEP081406510Th 429 737
121 LI:197433.5;2000SEP0883152247 429 792
121 LI: T 97433.5:2000SEP0870757065V 505 647
1
121 LI:197433.5:2000SEP0870753582V 550 776
1
122 LI:170604.1:2000SEP0881276258 1 257
122 LI:170b04.1;2000SEP0884371534 1 264
122 LI:170604.1:2000SEP086319666H 1 1 269
122 LI;170604, i :2000SEP084817706F6 6 579
122 LI:170604.1:2000SEP084817706H 1 6 281
123 LI:205057.3:2000SEP08g 1382854 173 486
123 LI:205057.3:2000SEP0884196450 289 485
123 LI:205057.3:2000SEP0883134110 98 485
123 LI:205057.3;2000SEP08831 61929 183 485
123 LI;205057.3:2000SEP0885855412 138 481
123 LI:205057.3:2000SEP0883299207 66 480
123 LI:205057.3:2000SEP08g 1269670 1 b2 480
123 LI:205057.3:2000SEP084367930H1 269 478
123 LI:205057.3;2000SEP0883744884 171 477
123 LI:205057.3;2000SEP0885445282 156 475
123 LI;205057.3:2000SEP087078181H1 119 466
123 LI:205057.3:2000SEP0882787551 101 453
123 LI:205057.3:2000SEP085445943H1 142 403
123 LI:205057.3;2000SEP086882994H1 102 653
123 LI;205057.3:2000SEP0871500229V1 38 633
123 LI;205057.3:2000SEP0871500709V1 15 599
123 LI:205057.3:2000SEP0871497289V 50 628
1
123 LI;205057.3:2000SEP0871496610V1 6l 626
123 LI:205057.3:2000SEP082890214F6 140 626
123 LI:205057.3;2000SEP0871496889V1 121 626
123 LI:205057.3:2000SEP0871497655V1 152 626
123 LI:205057.3;2000SEP0871495949V1 1 626
123 LI;205057.3:2000SEP087i685242V1 485 626
123 LI:205057.3:2000SEP0883230634 91 491
123 LI:205057.3:2000SEP084974269H1 269 486
124 LI:233795.1;2000SEP083082634H 1 1 285
125 LI:311197.1:2000SEP086803514H 1 503 984
125 LI:311 T 97.1:2000SEP086803514) 1 242 823
166
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Stark Stop
NO: ID
125 LI:311197,1:2000SEP086286909H2 316 799
125 LI:311197.1:2000SEP08g4568721 260 685
125 LI:311197,1:2000SEP08g3742689 209 684
125 LI:311197.1:2000SEP08g5365250 586 679
125 LI:311197.1:2000SEP08g5676607 451 679
125 LI:311197.1:2000SEP08g4111591 263 679
125 LI:311197.1:2000SEP08g2539496 299 678
125 LI:311197.1:2000SEP08g5232991 277 675
125 LI:311197,1:2000SEP08g5769156 224 677
125 LI:311197.1:2000SEP08g4299176 248 676
125 LI:311197.1:2000SEP08g3751723 261 676
125 LI:311197.1:2000SEP08g3090059 202 676
125 LI:311197,1:2000SEP08g5630519 273 675
125 LI:311197.1:2000SEP08574367477 354 545
125 LI:311197.1:2000SEP085743674H 1 1 297
125 LI:311197.1:2000SEP084127943H1 1 246
125 LI:311197,1:2000SEP08574367487 1 183
126 Li:441364.1:2000SEP0870611413V1 279 968
126 LI:441364,1:2000SEP0870610704V1 297 912
126 LI:441364.1:2000SEP08g6991360 349 914
126 LI:441364, l :2000SEP0870613163V 300 913
l
126 LI:441364.1:2000SEP0870610131V1 339 912
126 LI:441364,1:2000SEP087061 O 146V 323 912
l
126 LI:4413b4.1:2000SEP0870614931 V1 358 912
126 LI:4413b4,1:2000SEP0870613844V 261 912
1
126 LI:441364.1:2000SEP0870611321V1 367 906
126 LI:441364,1:2000SEP0870610435V 247 906
1
126 LI:4413b4.1:2000SEP0870613979V1 358 906
126 LI:441364.1:2000SEP0870613396V1 432 906
12b LI:441364.1:2000SEP0870613381V1 444 906
126 LI:4413b4.1:2000SEP0870611472V1 327 906
126 LI:441364,1:2000SEP0870613313V 386 906
1
126 LI:441364.1:2000SEP0870613466V1 259 906
126 LI:4413b4,1:2000SEP0870611896V 307 906
1
126 LI:441364.1:2000SEP087600446J 1 280 904
126 LI:441364,1:2000SEP0870611133V1 513 911
126 LI:44 i 364. T g5675202 435 884
:2000SEP08
126 LI:441364.1:2000SEP0870320441 D1 426 810
126 LI:441364,1:2000SEP0870610363V1 146 809
126 LI:441364.1:2000SEP0870611527V1 123 761
126 LI:441364,1:2000SEP0870320335D1 316 719
126 LI:4413b4.1:2000SEP087600446H 1 173 716
126 LI:441364.1:2000SEP0870358170D1 302 695
126 ' LI:4413b4,1:2000SEP0870320422D1 328 695
126 LI:441364.1:2000SEP0870320907D1 316 691
126 LI:441364.1:2000SEP0870610095V1 312 622
126 LI:4413b4.1:2000SEP0870613379V b5 597
1
126 LI:4413b4,1:2000SEP08_70613766V1 1 575
167
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
126 LI:4413b4.1:2000SEP0870611537V1 1 547
126 LI;441364.1;2000SEP0870614122V1 1 549
126 LI:441364.1:2000SEP0870612322V1 1 537
126 LI:4413b4.1:2000SEP0870610714V1 38 528
126 LI:441364.1;2000SEP0870613398V1 1 484
126 LI;441364.1;2000SEP082957262H1 221 472
126 LI;4413b4.1:2000SEP0870610973V1 1 423
126 LI:441364.1:2000SEP0870320525D1 4 413
126 LI;441364.1:2000SEP086820787H1 1 257
126 LI;441364.1:2000SEP082331551 R6 1 1 b7
126 LI:4413b4.1;2000SEP082331551 H 1 151
1
126 LI:441364.1:2000SEP08774009H 1 1 142
T 26 LI:441364.1:2000SEP08g5397181 427 884
126 LI;441364.1;2000SEP08g5769535 434 884
126 LI;441364.1:2000SEP08g5674965 457 884
126 LI:441364.1:2000SEP08g2959283 468 884
126 LI;441364.1:2000SEP08g2328116 590 884
126 LI:4413b4.1:2000SEP082666519F6 446 819
126 LI;441364.1:2000SEP082666519H 1 568 819
126 LI:4413b4.1;2000SEP0870319922D1 1 141
126 LI:4413b4.1:2000SEP081707105H1 1 134
127 LI:2103b7.6:2000SEP087664570) l 1 624
127 LI;210367.6:2000SEP087314310H1 247 812
127 LI;2103b7.6:2000SEP086393170H1 365 628
127 LI:2103b7.b:2000SEP087074279H1 396 950
127 LI:210367.6;2000SEP085961383H1 443 970
127 LI:2103b7.b:2000SEP085752701H1 487 971
127 LI:2103b7.6;2000SEP085753201 H1 489 927
127 LI;210367.6:2000SEP085536226H1 519 761
127 LI:210367.6;2000SEP087733323)2 553 1030
127 LI:210367.6:2000SEP081904192F6 568 1038
127 LI:2103b7.b:2000SEP082443147H1 618 846
127 LI;2103b7.6;2000SEP081904192H1 750 1038
127 LI:2103b7.6;2000SEP081904176H1 753 1038
127 LI;210367.b:2000SEP086772207H1 789 1055
127 LI;210367.6:2000SEP083881168H1 829 1104
127 LI;210367.6;2000SEP081963058H1 830 1024
127 LI:2103b7.b:2000SEP081963058R6 830 1039
127 LI:210367.b;2000SEP082719014H1 832 1088
127 LI:27 03b7.6:2000SEP082419884H 1 931 11 b0
127 LI;210367.b:2000SEP087757913)1 340 591
127 LI;210367.b;2000SEP087611830)1 699 1036
127 LI:2103b7.6:2000SEP087611869)1 627 1036
127 LI:210367.b:2000SEP087611869H 1 412 1003
127 LI:2103b7.b:2000SEP087611830H1 412 999
128 LI:238194.5:2000SEP086893225N 1 1 326
128 LI;238194.5:2000SEP087075927H1 28 387
128 LI:238194.5:2000SEP086912933) 1 50 656
16S
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
129 LI:039258.5;2000SEP087374628H1 1 497
129 LI;039258.5;2000SEP087260053H1 97 570
130 LI:1071842.1;2000SEP083295254H 1 172
1
130 LI;1071842.1:2000SEP086355170H1 1 313
130 LI:1071842.1;2000SEP086352664H1 1 237
130 LI;1071842.1:2000SEP084969508H1 5 150
130 LI:1071842.1:2000SEP088037001 H 17 463
1
130 LI:1071842.1:2000SEP086883378H1 23 424
130 LI;1071842.1;2000SEP08g2046377 47 436
130 LI;1071842.1;2000SEP086856114H 254 726
1
130 LI:1071842.1:2000SEP086856147H 254 786
1
130 LI:1071842.1:2000SEP08g2816492 351 798
130 LI:1071842.1;2000SEP085805030H1 380 646
130 LI:1071842.1:2000SEP084910380H1 394 709
131 LI:481356.3:2000SEP085675468H1 127 392
.131 LI:481356.3;2000SEP081646823H1 208 409
131 LI:48135b.3;2000SEP081699236H1 280 491
131 LI:481356.3:2000SEP08363353279 462 944
131 LI:481356.3:2000SEP086534988H1 27 414
131 LI:481356.3;2000SEP087271920H1 1 568
132 LI:103474.1:2000SEP086243379F8 1 524
132 LI:103474.1:2000SEP086243379H1 1 583
132 LI:103474.1:2000SEP08624337978 1 481
132 LI:103474.1;2000SEP08624798578 1 481
132 LI:103474.1:2000SEP08~ 624676278 1 467
132 LI:103474.1;2000SEP086247985H 1 558
1
132 LI:103474.1:2000SEP086246762H1 1 583
132 LI:103474.1:2000SEP086246762F8 1 526
132 LI:103474.1;2000SEP086243872H 1 598
1
132 LI:103474.1;2000SEP086247985F8 9 520
132 LI:103474.1:2000SEP082895966H 26b 560
1
133 LI:1073020,.10:2000SEP087925042H1 1 638
133 LI:1073020.10:2000SEP087432730H 505 950
1
133 LI;1073020.10:2000SEP0870043051 912 1415
D1
133 LI:1073020.10:2000SEP084599916H1 935 1206
134 LI;000874.1:2000SEP08676441H1 1983 2257
134 LI:000874.1;2000SEP08744437971 1567 2180
134 LI:000874.1:2000SEP086445251 T8 1627 2177
134 LI:000874.1:2000SEP08744443071 1648 2182
134 LI:000874.1;2000SEP08346856476 1858 2235
134 LI:000874.1:2000SEP083468564F6 1865 2219
134 LI;000874.1:2000SEP083468564H1 1865 2138
134 LI:000874.1:2000SEP08417806476 1870 2250
134 LI:000874.1:2000SEP08g2836242 1892 2254
134 LI:000874.1;2000SEP08g4147256 1894 2283
134 LI:000874.1:2000SEP08g3678872 1895 2286
134 LI:000874.1:2000SEP084515055H1 1904 2153
134 LI:000874.1:2000SEP08~ 672386H1 1983 2242
169
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6~ Template ID Component Start Stop
ID NO: ID
134 LI:000874.1;2000SEP08676441 T6 1983 2242
134 LI;000$74.1;2000SEP086445251 H1 339 460
134 LI;000874.1:2000SEP086445251 F8 339 900
134 LI:000874.1:2000SEP0871215968V1 520 667
134 LI:000874.1:2000SEP084178064H1 520 770
134 LI;000874.1:2000SEP084178064F6 520 1026
134 LI:000874.1;2000SEP086000160H 1 968 1466
134 LI:000874.1:2000SEP086308558N1 967 1528
134 LI;000874.1:2000SEP086558026H1 1399 1961
134 LI:000874.1;2000SEP084311435H1 1417 1572
134 LI;000874.1;2000SEP086560545H1 1429 1940
134 LI;000874.1:2000SEP087073540H1 1 329
134 LI;000874.1;2000SEP087287283H1 82 396
134 LI;000874.1;2000SEP084711204H1 92 226
134 LI:000874.1:2000SEP086444020H1 95 bll
134 LI:000874.1:2000SEP08676441 R6 1983 2283
134 LI;000874.1;2000SEP08g6043865 2105 2283
134 LI;000874.1;2000SEP08g5663173 2146 2275
135 LI:037298.2;2000SEP08g4310397 2252 2724
135 , LI;037298.2:2000SEP0884740199 2272 ' 2727
135 LI:037298.2:2000SEP0883778159 2286 . 2735
135 LI;037298.2;2000SEP0884991293 2298 2741
135 LI:037298.2:2000SEP08g 1289893 2301 2733
135 LI:037298.2:2000SEP0883015750 2350 2731
1,35 LI;037298.2;2000SEP0883181863 2351 2729
135 LI:037298.2:2000SEP0883755500 2356 2727
135 LI:037298.2:2000SEP085656848H1 2359 2609
135 LI;037298.2;2000SEP0887237i78 2367 2721
135 LI;037298.2:2000SEP0885636414 2385 2721
135 LI:037298.2:2000SEP088819400 2388 2739
135 LI:037298.2;2000SEP0884310951 1 452
135 LI;037298.2:2000SEP0884684224 i 480
135 LI:037298.2;2000SEP0884175610 1 485
135 LI:037298.2;2000SEP0886700878 2 277
135 LI;037298.2:2000SEP0886699894 2 439
135 LI:037298.2;2000SEP0886701663 2 529
135 LI:037298.2:2000SEP0885362582 10 485
135 LI:037298.2;2000SEP0884175608 19 485
135 LI;037298.2:2000SEP0885632072 32 487
135 LI;037298.2;2000SEP0883095847 147 231
135 LI:037298.2:2000SEP087177795H1 189 736
135 LI:037298.2:2000SEP0870850933V1 386 869
135 LI:037298.2;2000SEP0870849483V1 386 946
135 LI:037298.2:2000SEP0870853177V1 386 798
135 LI;037298.2:2000SEP084293646F6 386 787
135 LI:037298.2:2000SEP0870853110V1 386 636
135 LI;037298.2:2000SEP084293646H1 386 631
i 35 LI:037298.2:2000SEP087085 T 283V 502 855
1
170
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
135 LI:037298,2:2000SEP08g3250513 507 729
135 LI:037298,2:2000SEP0870854055V1 562 923
135 LI:037298.2:2000SEP0870853228V1 615 1182
135 LI:037298.2:2000SEP0870852672V1 642 1270
135 LI:037298.2:2000SEP0870853222V1 719 1053
135 LI:037298.2:2000SEP0870849522V1 776 1282
135 LI:037298.2:2000SEP0870803032V1 840 1048
135 LI:037298,2:2000SEP0870851817V1 854 1441
135 LI:037298.2:2000SEP0870853296V1 854 1426
135 LI:037298.2:2000SEP0870852005V1 914 1532
135 LI:037298,2:2000SEP0870852305V1 953 1588
135 LI:037298,2:2000SEP0870853794V1 974 1492
135 LI:037298.2:2000SEP086342011 H 999 1579
1
135 LI:037298,2:2000SEP08g676942 1016 1270
135 LI:037298.2:2000SEP08g574848 1016 1270
135 LI:037298,2:2000SEP08g870484 1017 1330
135 LI:037298.2:2000SEP08g824450 1017 1295
135 LI:037298.2:2000SEP08g774466 1018 1327
135 LI:037298,2:2000SEP0870848993V1 1023 1528
135 LI:037298,2:2000SEP0870853573V1 1031 1559
135 LI:037298.2:2000SEP08g774465 1045 1370
135 LI:037298.2:2000SEP0870851133V1 1049 1727
135 LI:037298.2:2000SEP0870854034V1 1109 1484
135 LI:037298.2:2000SEP08g3958586 1134 1556
135 LI:037298.2:2000SEP0870849240V1 1134 1774
135 LI:037298.2:2000SEP0870850404V1 1152 1437
135 LI:037298.2:2000SEP08.70851556V1 1256 1728
135 LI:037298.2:2000SEP0870850987V1 1291 1739
135 LI:037298.2:2000SEP0870853756V1 1343 1915
135 LI:037298,2:2000SEP0870849160V1 1385 1894
135 LI:037298.2:2000SEP0870853632V1 1398 1913
135 LI:037298.2:2000SEP0870854612V1 1448 1913
135 LI:037298.2:2000SEP084293646R6 1483 1913
135 LI:037298.2:2000SEP083973584F8 1881 2385
135 LI:037298.2:2000SEP083973584H 1 1881 2151
135 LI:037298.2:2000SEP083973584F6 1881 2241
135 LI:037298.2:2000SEP08g 1321459 1981 2410
135 LI:037298.2:2000SEP084323149H 1 1992 2162
135 LI:037298,2:2000SEP08g 1200548 1997 2284
135 LI:037298.2:2000SEP082270683R6 2019 2399
135 LI:037298.2:2000SEP082270683H 1 2019 2269
135 LI:037298.2:2000SEP08g1188713 2034 2178
135 LI:037298,2:2000SEP086129689H1 2064 2620
135 LI:037298.2:2000SEP086125735H 1 2065 2629
135 LI:037298.2:2000SEP08397358478 2088 2712
135 LI:037298.2:2000SEP081003739H1 2168 2404
135 LI:037298.2:2000SEP08g870485 2391 2738
135 LI:037298,2:2000SEP08g824261 2424 2738
_. _ -171
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
135 LI:037298.2:2000SEP08g824262 2466 2738
735 LI:037298.2;2000SEP08g3755677 2470 2727
135 LI:037298.2:2000SEP08g1188714 2483 2740
135 LI;037298.2;2000SEP08g567521 2486 2721
135 LI:037298.2;2000SEP08g671199 2510 2721
135 LI:037298.2;2000SEP08g1148845 2615 2721
135 LI:037298.2:2000SEP08g2842176 2624 2731
136 LI:422901.1:2000SEP087669538H1 1 487
136 LI:422901.1:2000SEP088009135H1 2 595
136 LI:422901.1:2000SEP088007884H2 1 564
136 LI:422901. 7 :2000SEP088011553H 1 1 559
136 LI:422901.1;2000SEP088010625H1 1 592
136 LI:422901.1:2000SEP088008669H 1 1 471
136 LI:422901.1:2000SEP088009653H1 2 489
136 LI:422901.1:2000SEP084586653H1 9 173
136 LI:422901.1:2000SEP0870481183V1 10 562
136 LI:422901.1:2000SEP084659525H 1 14 200
136 LI:422901.1;2000SEP0870466352V1 198 686
136 LI:422901.1;2000SEP0870471460V1 226 486
136 LI;422901.1;2000SEP0870465222V1 223 700
136 LI:422901.1;2000SEP0870479783V1 241 359
136 LI;422901.1:2000SEP0870495442V1 286 390
136 LI:422901.1;2000SEP0870464419V1 298 874
136 LI:422901.1:2000SEP0870466486V7 314 937
'
136 LI:422901.1:2000SEP0870481279V1 353 ' 889
136 LI;422901.1;2000SEP0870481193V1 359 921
136 LI:422901.1:2000SEP0870467356V1 361 898
~
136 LI:422901.1;2000SEP0870472724V 399 747
1
136 LI:422901.1:2000SEP0870469921V1 418 846
136 LI;422901.1:2000SEP0870496406V1 461 600
136 LI:422901.1:2000SEP0870468552V 843 1268
1
136 LI:422901.1;2000SEP0870469892V1 887 1128
.
136 LI:422901.1:2000SEP0870469095V1 887 1126
136 LI:422901.1:2000SEP0870465027V 905 1183
1
136 LI:422901.1;2000SEP0870467528V1 922 1530
136 LI:422907.1:2000SEP0870466219V1 462 659
136 LI:422901.1;2000SEP0870481675V1 469 1071
136 LI:422901.1:2000SEP0870480075V1 479 632
136 LI;422901.1;2000SEP0870466776V1 508 1009
136 LI:42290 T .1:2000SEP0870481912V 507 1 T
1 03
136 LI;422901.1:2000SEP0870465242V1 509 1091
136 LI:422901.1;2000SEP0870468287V1 547 1127
136 LI:422901.1:2000SEP0870467133V1 559 1182
136 LI:422901.1:2000SEP0870479655V1 559 1116
136 LI;422901.1;2000SEP0870466676V1 559 1114
136 LI:422901.1;2000SEP0870479011 V 569 797
1
136 LI;422901.1:2000SEP0870467578V1 584 1141
136 LI;422901.1:2000SEP0870465558V1 583 798
172
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
136 LI:422901.1:2000SEP0870481928V1 591 1121
136 LI:422901.1:2000SEP0870467547V1 604 1003
136 LI:422901.1:2000SEP0870465569V 611 1077
1
136 LI:422901.1:2000SEP0870467459V1 638 1003
136 LI:422901.1;2000SEP0870466607V1 631 891
136 LI:422901.1:2000SEP0870469051 V1 621 1183
136 LI:422901.1;2000SEP08580842378 648 1127
136 LI:422901.1;2000SEP0870479176V1 660 1111
136 LI:422901.1:2000SEP0870464261 V1 694 1129
136 LI:422901.1;2000SEP0870480212V1 692 861
136 LI:422901,1:2000SEP0870481058V 692 862
1
136 LI;422901.1:2000SEP0870480415V1 691 859
136 LI;422901.1;2000SEP0870479889V1 706 912
136 LI:422901.1:2000SEP0870464582V1 708 967
136 LI:422901.1:2000SEP0870466381V1 737 1254
136 LI;422901.1;2000SEP0870464506V1 780 1299
136 LI:422901.1:2000SEP0870466025V1 777 1317
136 LI;422901.1;2000SEP0870467618V1 791 1399
136 LI;422901.1;2000SEP084659870H1 799 1051
136 LI:422901.1:2000SEP0870477354V1 807 1308
136 LI;422901.1:2000SEP0870479682V1 816 ' 1005
136 LI;422901.1;2000SEP0870468490V1 846 1157
136 LI:422901.1:2000SEP0870465557V1 922 1357
136 LI:422901.1:2000SEP0870481594V1 939 1104
136 LI;422901.1:2000SEP0870478404V1 944 1104
136 LI;422901.1:2000SEP0870479902V1 979 1275
136 LI;422901.1;2000SEP0870465042V1 987 1576
136 LI;422901.1;2000SEP0870466835V1 998 1336
136 LI:422901.1:2000SEP0870467624V1 1052 1571
136 LI:422901.1;2000SEP0870477595V1 1075 1587
136 LI;422901.1:2000SEP0870468737V1 1080 1586
136 LI:422901.1:2000SEP0870481023V1 1089 1186
136 LI:422901.1:2000SEP084532750H 1 1297 1487
137 LI:345815.1:2000SEP081428801 F6 473 911
137 LI:345815.1;2000SEP082768967H1 772 1020
137 LI:345815.1:2000SEP087586434H 1 859 T 345
137 LI:345815.1;2000SEP081428801 T6 963 1574
137 LI:345815.1:2000SEP082174927H1 1272 1516
137 LI:345815.1:2000SEP0855013966) 1396 1867
1
137 LI:345815.1:2000SEP08g2329596 1396 1762
137 LI;345815.1;2000SEP0855013966H1 1507 2097
137 LI:345815.1:2000SEP087974188H1 1523 2001
137 LI;345815.1;2000SEP0890144686 1622 2104
137 LI:345815.1:2000SEP0890144681 1622 2163
137 LI:345815.1:2000SEP08901446H 1 1622 1911
137 LI:345815.1:2000SEP08g1164340 1653 2030
137 LI:345815.1;2000SEP081719224H 1 1806 2015
137 LI:345815.1:2000SEP08808303H 1 1865 2104
-
_
173
,
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
137 LI:345815. T :2000SEP087729928) 1 282 357
137 LI:345815.1:2000SEP083154867H1 1 270
.
137 LI:345815.1;2000SEP083482727H1 10 338
137 LI;345815.1:2000SEP085688762F6 37 340
137 LI:345815.1;2000SEP087151710H1 57 524
138 LI:1072014.2;2000SEP0871622669V1 236 768
138 LI;1072014.2:2000SEP085200035H1 604 676
138 LI:1072014.2:2000SEP08g6198267 1 405
138 LI:1072014.2:2000SEP0871626629V1 236 918
138 LI;1072014.2:2000SEP0871534676V1 236 728
i 38 LI;1072014.2:2000SEP0871625614Vi 236 791
138 LI;1072014.2:2000SEP0871537443V1 236 830
138 LI:1072014.2:2000SEP0871622576V1 236 778
138 LI:1072014.2:2000SEP0871625608V1 236 789
138 LI;1072014.2:2000SEP083253807H1 236 491
138 LI:1072014.2;2000SEP08325380786 236 660
138 LI:1072014.2:2000SEP0871534821 V1 236 647
138 LI:1072014.2;2000SEP0871553072V1 236 604
139 LI:333138.3;2000SEP08702496i3V1 1 197
139 LI:333138.3:2000SEP083269702H1 8 253
~
139 LI:333138.3:2000SEP0870249634V1 141 373
139 LI:333138.3:2000SEP084023624H1 1 285
140 LI:414253.1:2000SEP087188650H1 300 787
140 LI:414253.1;2000SEP0871033390V1 1798 2223
140 LI;414253, i :2000SEP083471178H1 43 304
i 40 LI:414253. i ;2000SEP08698354588 242 544
140 LI;414253.1:2000SEP086983545H1 242 544
140 LI:414253.1:2000SEP087077140H1 261 831
140 LI:414253:1:2000SEP083758801 H1 279 490
140 LI:414253.1:2000SEP086888331 H 25 338
1
140 LI:414253.1:2000SEP087076931 H 32 575
1
140 LI:414253.1:2000SEP087192833H2 38 230
140 LI:414253.1;2000SEP08g6302480 39 312
140 LI:414253.1:2000SEP08g872877 1787 2041
140 LI;414253.1:2000SEP087179630H1 1743 2246
140 LI:414253.1:2000SEP086272220H2 1776 2332
140 LI;414253.1;2000SEP0871237924V1 1782 2312
140 LI:414253.1:2000SEP0862309686 1786 2073
140 LI:414253.1;2000SEP08623096H1 1786 2020
140 LI:414253.1:2000SEP08g573153 1786 2116
140 LI:414253,1;2000SEP086055925H 1 1422 1563
140 LI;414253.1:2000SEP0871236732V1 1480 2057
140 LI;414253.1:2000SEP0871021573V1 1484 2066
140 LI;414253.1:2000SEP0871019327V1 1485 2089
140 LI:414253.1;2000SEP082809615H1 1501 1590
140 L1:414253.1:2000SEP0871237462V1 1508 2033
140 LI:414253.1;2000SEP0871033503V 1526 1717
1
140 LI:414253.1:2000SEP0871020456V1 1547 2040
174
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
140 LI;414253.1:2000SEP086882657H1 1572 2123
140 LI:414253.1;2000SEP0871022138V1 1579 2095
140 LI;414253.1:2000SEP087T240477VT 1598 1700
140 LI:414253.1;2000SEP085259755H 1 1700 1866
140 LI;414253.1:2000SEP0871020549V1 1732 2152
140 LI:414253.1:2000SEP086894886) 1 1734 2325
140 LI:414253.1:2000SEP0871020631 V 1920 2155
1
140 LI:414253.1:2000SEP087073747H1 1 544
140 LI:414253.1;2000SEP086765077H1 1900 2426
140 LI;414253.1;2000SEP086894886H1 1905 2510
i40 LI:414253.1:2000SEP0877022463V1 1906 2393
140 LI;414253.1:2000SEP08~71021156V1 1911 2555
140 LI:414253.1;2000SEP0871019356V1 1080 1765
140 LI:414253.1:2000SEP0871022935V1 1103 1573
140 LI;414253.1:2000SEP0871019614V1 1202 1344
140 LI;414253.1:2000SEP087115421H1 1209 1756
140 LI:414253.1:2000SEP087112040H2 1209 1703
140 LI:414253.1:2000SEP087112740H2 1209 1782
140 LI;414253.1:2000SEP0871019475V1 1224 1515
140 LI:414253.1:2000SEP0871021555V1 1224 1515
140 LI:414253.1:2000SEP084707917H1 1257 1525
140 LI:414253.1;2000SEP084692502H 1 1267 1520
140 LI:414253.1;2000SEP0871033330V1 1304 1472
140 LI:414253.1;2000SEP081945748H 1 1323 1553
140 LI;414253.7:2000SEP08g771044 1331 1712
140 LI:414253.1:2000SEP081543574H1 1358 1573
140 LI:414253.1:2000SEP08154357486 1358 1885
140 LI:414253.1:2000SEP087068113H1 1362 1955
140 LI:414253.1:2000SEP0871020257V1 1391 1920
140 LI:414253.1:2000SEP08925902H 1 1392 1585
140 LI:414253.1;2000SEP0871022851 V1 1405 1966
140 LI;414253.1;2000SEP0867580788 1415 1554
140 LI:414253.1:2000SEP08675807H1 1415 1554
140 LI:414253.1:2000SEP0867580786 1415 1554
140 LI;414253.1:2000SEP087292014H1 1052 1524
140 LI:414253.1;2000SEP082039968H1 1069 1346
140 LI:414253.1:2000SEP0871021025V 1072 1573
1
140 LI:414253.1;2000SEP085683551 F6 1000 1379
140 LI;414253.1;2000SEP0871020667V1 985 1259
140 LI;414253.1:2000SEP0871019368V1 1000 1454
140 LI:414253.1:2000SEP085683551 H 1000 1272
1
140 LI:414253.1:2000SEP0867580787 1415 1554
140 LI;414253.1:2000SEP0871034573V1 1830 1976
140 LI:414253.1;2000SEP0871237928V1 1842 2370
140 LI:414253.1:2000SEP081397602H1 686 775
140 LI:414253.1;2000SEP087287151 H 750 1231
1
140 LI:414253.1:2000SEP081417182H 1 536 764
140 LI:414253.1:2000SEP086888331)1 930 1518
175
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
140 LI:414253.1:2000SEP0871020886V1 1837 2393
140 LI:4i4253.1:2000SEP087997345H1 346 609
140 LI:414253.1:2000SEP08623096T6 1940 2533
140 LI:414253.1;2000SEP084343090H1 1947 2034
140 LI;414253.1;2000SEP085965294F8 1990 2570
140 LI:414253.1;2000SEP081543574Th 2029 2533
140 LI:414253.1:2000SEP082044247H1 2054 2322
140 LI;414253.1:2000SEP08204424786 2054 2393
140 LI;414253.1:2000SEP087060219H1 2077 2569
140 LI:414253.1;2000SEP088315040 2112 2572
140 LI:414253.1:2000SEP0886228972 2113 2569
140 LI;414253.1:2000SEP0871019196V1 2131 2570
140 LI:414253.1;2000SEP0871021676V1 2121 2589
140 LI;414253.1:2000SEP087578441 H2 2137 2324
140 LI;414253.1:2000SEP082043595H1 2214 2393
140 LI:414253.1:2000SEP088872878 2238 2579
140 LI;414253.1;2000SEP088819699 2270 2582
140 LI:414253.1:2000SEP082407286H1 2294 2522
i 40 LI:414253.1;2000SEP086882657) 1 2315 2508
140 LI:414253.1;2000SEP082157543H 1 2430 2526
140 LI:414253.1:2000SEP082157543T6 2431 2532
140 LI:414253.1;2000SEP082157543F6 2430 2569
140 LI:414253.1:2000SEP08674031 6H 2458 2560
1
141 LI:40b389.1:2000SEP086870105H1 1151 1588
141 LI;40b389.1;2000SEP086870968H l 1155 1673
141 LI:406389.1;2000SEP0883805640 1188 1567
141 LI:406389. i :2000SEP0885b 1297 1227 1562
141 LI:40b389.1;2000SEP088795551 1252 1573
141 LI;406389.1;2000SEP088683370 1276 1562
141 LI:40b389.1;2000SEP088214i 803 1405 1567
141 LI;40b389.1:2000SEP084788627Th 1454 1521
141 LI:406389.1:2000SEP084766304H 1 699 971
141 LI:406389.1:2000SEP08921701H1 930 1252
141 LI:40b389.1:2000SEP087091595H1 932 1469
141 LI:40b389.1:2000SEP084766304Th 1133 1520
141 LI;40b389.1;2000SEP0883308722 1149 1562
141 LI:40b389.1;2000SEP0884264177 1 481
141 LI:40b389.1:2000SEP086768921 J 10 579
1
141 LI;40b389.1:2000SEP086767668) 1 10 471
141 LI:40b389.1:2000SEP083787648H1 391 669
141 LI:40b389.1;2000SEP088709143 404 717
141 LI:406389.1:2000SEP088570324 420 741
141 LI:40b389.1:2000SEP088694218 516 743
141 LI:40b389.1;2000SEP083574983N 1 651 940
141 LI;406389.1:2000SEP0881809833 651 913
141 LI:40b389.1:2000SEP084766304F6 699 1072
142 LI:1086171.1:2000SEP086796022F8 1 550
142 LI:108b171.1:2000SEP086796022H1 1 575
176
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
142 LI:108b171.1:2000SEP08679602278 1 463
143 L!;198782.4:2000SEP087753061 J 4352 5049
1
143 LI:198782.4:2000SEP085778688H1 4358 4623
143 LI;198782.4;2000SEP08626505H1 4364 4633
143 LI;198782.4:2000SEP083620066H1 4365 4655
143 LI:198782.4:2000SEP086206412H1 4377 4941
143 LI:198782.4;2000SEP082969374H1 4386 4695
143 LI:198782.4;2000SEP081208501 R1 4417 4986
143 LI;198782.4:2000SEP081208501 H 4417 4656
1
143 LI;198782.4;2000SEP0885593068 4436 4933
143 LI;198782.4:2000SEP0872278681 4460 5054
143 LI:198782.4:2000SEP08722786H1 4460 4749
143 LI:198782.4:2000SEP085196343H1 4466 4740
143 LI;198782.4:2000SEP08g 1802558 3537 3893
143 LI:198782.4:2000SEP088124049H1 3532 4154
143 LI: i 98782.4;2000SEP084783988H 1 4687 4927
143 LI:198782.4:2000SEP083434336H 1 4709 4904
143 LI;198782.4;2000SEP087749827) 1 4719 5327
143 LI:198782.4;2000SEP083379948H1 3338 3615
143 LI:198782.4:2000SEP082174420F6 3426 3943
143 LI;198782.4;2000SEP082174420H1 3427 3668
143 LI:198782.4:2000SEP086348086H1 3449 3737
143 LI;198782.4;2000SEP087634241 J 3466 4048
1
143 LI:198782.4:2000SEP084289769H 1 3503 3763
143 LI;198782.4:2000SEP084174741 H 3506 3806
1
143 LI:198782.4;2000SEP085980307H1 3522 3822
143 LI;198782.4:2000SEP085088691 H 3529 3784
1
143 LI:198782.4:2000SEP08407900H1 4804 5045
143 LI:198782.4:2000SEP082972377H1 4812 5119
143 LI:198782.4;2000SEP08220b290Hi 4845 5110
143 LI:198782.4:2000SEP08177338676 4848 5323
143 LI;198782.4:2000SEP08177338686 4849 5361
143 LI;198782.4:2000SEP081916583H 1 4849 5112
143 LI:198782.4;2000SEP0881873598 4853 5354
143 LI:198782.4:2000SEP0885236486 4875 5354
i 43 Ll: i 98782.4;2000SEP0886575406 4888 5354
143 LI;198782.4:2000SEP0885812818 4890 5354
143 LI:198782.4:2000SEP083382159H1 1 251
143 LI:198782.4;2000SEP08" 84435439 1 470
143 LI;198782.4:2000SEP083349345H 1 3 220
143 LI:198782.4:2000SEP08g 1203045 4891 5190
143 LI:198782.4:2000SEP0883596839 4895 5363
143 LI:198782,4:2000SEP0885426010 4898 5354
143 LI:198782.4:2000SEP083620979H1 4899 5185
143 LI:198782.4:2000SEP08290991 OH 4323 4575
1
143 LI: i 98782.4:2000SEP08g 1982687 4332 4617
143 LI:198782.4:2000SEP08.6361528H2 3566 4075
143 LI:198782.4:2000SEP083687346H1 3633 3907
-
177
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6~ Template ID Component Start Stop
ID NO: ID
143 LI:198782,4:2000SEP084691212H1 3635 3899
143 LI:198782.4:2000SEP082972069H 1 3637 3959
143 LI:198782,4:2000SEP087698932H 1 3722 4404
143 LI:198782,4:2000SEP08g783276 3735 3943
143 LI:198782.4:2000SEP084891359H 1 3793 4054
143 LI:198782,4:2000SEP084780573H1 3803 4015
143 LI: i 98782.4:2000SEP085377536H 1 3827 4061
143 LI:198782,4:2000SEP082813773F6 3837 4403
143 LI:198782,4:2000SEP082813773H 1 3838 4118
143 LI:198782.4:2000SEP082294301 R6 3886 4404
143 L1:198782,4:2000SEP082294301 H 3886 4149
1
143 LI:198782.4:2000SEP082653851 H1 3901 4196
143 LI.198782.4:2000SEP08662328H1 3944 4198
143 LI:198782,4:2000SEP083529451 H1 3961 4274
143 LI:198782.4:2000SEP081878246F6 3967 4265
i43 LI:198782,4:2000SEP081878246H1 3967 4168
143 LI: i 98782,4:2000SEP082540450H 1 3971 4229
143 LI:198782.4:2000SEP08880264H1 3994 4297
143 LI:198782.4:2000SEP0875397581 4022 4558
143 LI:198782.4:2000SEP086518464H 1 4078 4679
143 LI:198782.4:2000SEP081393486H1 4078 4261
743 LI:198782,4:2000SEP083243025H1 4078 4261
143 LI:198782,4:2000SEP08753975H1 4078 4220
143 LI:198782,4:2000SEP084775758H1 4081 4352
143 L1:198782.4:2000SEP081415015H 1 4122 4397
143 L1:198782.4:2000SEP086309993H1 ~ 4150 4680
143 LI:198782,4:2000SEP08g1873712 4182 4584
143 LI:198782.4:2000SEP081463313H 1 4205 4453
143 LI:198782,4:2000SEP082287535H1 4218 4356
143 LI:198782,4:2000SEP082957618H1 4220 4382
143 LI: i 98782.4:2000SEP084539614H 1 4252 4432
143 LI:198782,4:2000SEP082608763H2 4252 4495
143 LI:198782,4:2000SEP086518447H1 4289 4679
143 LI:198782,4:2000SEP081512393H1 4305 4507
143 LI:198782,4:2000SEP085518530H1 4311 4478
143 LI:198782.4:2000SEP086328279H1 4602 5114
143 L1:198782.4:2000SEP086335657H1 4602 4755
143 LI:198782.4:2000SEP081495153H1 4620 4852
143 LI:198782,4:2000SEP083727136H1 4641 4951
143 LI:198782,4:2000SEP084216627H1 4652 4930
143 LI:198782.4:2000SEP082298651H1 4655 4923
143 LI:198782.4:2000SEP082924824H 1 4658 4936
143 LI:198782,4:2000SEP083733962H1 8 301
143 LI:198782.4:2000SEP08g6946984 35 481
143 LI:198782.4:2000SEP081993570F6 38 517
143 LI: i 98782.4:2000SEP081993470H 1 38 296
143 LI:198782.4:2000SEP08~ 1993570H 38 248
1
143 LI:198782,4:2000SEP087727533J1 44 273
178
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
143 LI;198782.4:2000SEP087727533H 1 59 277
143 Ll: i 98782.4:2000SEP083232864H 1 74 351
143 LI:198782.4:2000SEP08g2575182 82 446
143 LI:198782.4:2000SEP085375624H1 116 358
143 LI;198782.4;2000SEP085605969H1 183 452
143 LI:198782.4:2000SEP088024696) 1 227 856
143 LI:198782.4:2000SEP08g4458155 313 761
143 LI;198782.4;2000SEP08g1933416 318 812
143 LI:198782.4:2000SEP08g 1924157 367 724
143 LI:198782.4:2000SEP08402351 6H 385 . 691
1
143 LI:198782.4:2000SEP084165481 H 386 672
1
143 LI:198782.4:2000SEP084172681 H 385 648
7
143 LI:198782.4:2000SEP08g2219275 391 797
143 LI:198782.4:2000SEP087184257H1 458 1043
143 LI:198782.4:2000SEP08g 1526024 578 1058
143 LI:198782.4;2000SEP0881266311 628 897
143 LI;198782.4;2000SEP088723408 675 963
143 LI:198782.4;2000SEP0883070857 735 1217
143 LI;198782.4:2000SEP08g 1933415 735 1201
143 LI:198782.4;2000SEP087213803H1 763 1189
143 LI:198782.4;2000SEP082433334H1 775 995
143 LI:198782.4:2000SEP0885854526 781 1214
143 LI:198782.4:2000SEP0883784943 785 1217
143 LI:198782.4:2000SEP08g 1923460 791 1209
143 LI;198782.4:2000SEP084270541 H 818 1075
1
143 LI:198782.4;2000SEP0881525917 830 1214
143 LI:198782.4:2000SEP087090529H1 835 1392
143 LI:198782.4:2000SEP088724366 849 1208
143 LI:198782.4:2000SEP0885233108 870 1208
143 LI:198782.4;2000SEP0882357872 916 1207
143 LI;198782.4;2000SEP0882779400 923 1211
143 LI:198782.4:2000SEP083069437H 1 11 b2 1437
143 LI;198782.4:2000SEP083069437F6 1162 1301
143 LI;198782.4:2000SEP087042063H 1 1221 1772
143 LI:198782.4:2000SEP08g 1481929 1235 1691
143 LI:198782.4:2000SEP087743465H1 1338 1963
143 LI:198782.4:2000SEP087740905H 1 1586 2208
143 LI:198782.4:2000SEP0882715455 1716 2058
143 LI:198782.4:2000SEP083094122F6 1730 2149
143 LI:198782.4:2000SEP083094122H1 1730 2002
143 LI:198782.4:2000SEP086943391 H 1779 2275
l
143 LI:198782.4;2000SEP086782233H1 1841 2390
143 LI:198782.4:2000SEP0882000179 1954 2215
143 LI:198782.4:2000SEP0882000178 1954 21 b4
143 LI;198782.4:2000SEP087740905) 1 1965 2586
143 LI:198782.4;2000SEP086723501H1 2114 2553
143 LI: i 98782.4;2000SEP088124509H i 2 i 39 279
i
143 LI:198782.4:2000SEP087727032H1 2166 2590
-
179
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
143 LI:198782.4;2000SEP08759i9b9H1 2274 2703
143 LI:198782.4:2000SEP086782233) 1 2385 3017
143 LI:198782.4:2000SEP08171482H1 2429 2642
143 LI:198782.4:2000SEP088003320H1 2718 3298
143 LI:198782.4;2000SEP088004370H1 2718 3203
143 LI:198782.4:2000SEP086607576H1 2819 3350
143 LI:198782.4:2000SEP087698932) 1 2850 3322
143 LI:198782.4;2000SEP082069005H1 2880 3155
143 LI;198782.4:2000SEP087387576H1 2967 3545
143 LI:198782.4;2000SEP087730026)1 2990 3537
143 LI:198782.4:2000SEP086751302H1 2992 3574
143 LI:198782.4:2000SEP087634241 H 3019 3581
1
143 LI:198782.4:2000SEP083605946H1 3040 3363
143 LI:198782.4:2000SEP082717553F6 3050 3596
143 LI:198782.4:2000SEP082717553H1 3050 3319
143 LI:198782.4:2000SEP082670755H1 3050 3294
143 LI:198782.4:2000SEP08306943786 3069 3550
143 LI:198782.4;2000SEP08g6438212 3118 3588
143 LI:198782.4:2000SEP0843324b7Hi 3184 3274
143 LI;198782.4;2000SEP082723120H1 3201 3460
143 LI:198782.4:2000SEP087749827H1 3236 3784
143 LI:198782.4;2000SEP086584914H1 3293 3604
143 LI:198782.4:2000SEP08g825991 3338 3744
143 LI:198782.4:2000SEP086172567H1 4733 5040
143 LI;198782.4;2000SEP08281377376 4735 5316
143 LI:198782.4:2000SEP082222943H 1 4757 5024
143 LI:198782.4:2000SEP08271755376 4769 5319
143 LI:198782.4:2000SEP08539612471 4769 5268
i 43 LI: i 98782.4:2000SEP084650454H 1 4769 5047
143 LI.198782.4:2000SEP08g1238470 4772 5001
143 LI:198782.4:2000SEP08g867285 4793 5139
143 LI:198782.4:2000SEP08g789023 4792 5039
143 LI:198782.4:2000SEP0840790086 4804 5101
143 LI:198782.4:2000SEP087345790H1 4482 5102
143 LI;198782.4;2000SEP084139428H1 4480 4798
143 LI:198782.4:2000SEP081910341 H 4543 4796
i
143 LI:198782.4:2000SEP084893581 H 4581 4859
1
143 LI:198782.4:2000SEP085207501 H1 4581 4768
143 LI:198782.4:2000SEP085153162H1 4592 4710
143 LI:198782.4:2000SEP087753061 H 3542 4095
1
143 LI:198782.4:2000SEP088124058H1 3532 4141
143 LI:198782.4:2000SEP08g3988960 4901 5362
143 LI:198782.4:2000SEP08309412276 4904 5307
143 LI;198782.4:2000SEP08g5741223 4909 5357
143 LI:198782.4;2000SEP08g3038341 4910 5354
143 LI;198782.4:2000SEP08g3770738 4911 5359
143 LI:198782.4:2000SEP08217442076 4913 5315
143 LI:198782.4:2000SEP08_g38_42608 4923 5354
___
180
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
143 LI:198782.4:2000SEP081485957H1 4930 5223
143 LI:198782.4;2000SEP0840790076 4948 5329
143 LI:198782.4:2000SEP0884737776 4954 5354
143 LI:198782.4:2000SEP082294301 T6 4965 5316
143 LI:198782.4:2000SEP0882445541 4968 5361
143 LI;198782.4:2000SEP082569859H 1 4983 5254
143 LI;198782.4:2000SEP0882401656 4986 5354
143 LI:198782.4;2000SEP084314015H1 5000 5319
143 LI:198782.4:2000SEP0882902280 5002 5354
143 LI:198782.4;2000SEP0885862936 5010 5385
143 LI;198782.4:2000SEP081371538H 1 5020 5225
143 LI:198782.4;2000SEP082422265H1 5021 5291
143 LI:198782.4:2000SEP08366679H1 5033 5300
143 LI;198782.4:2000SEP0885546865 5044 5355
143 LI:198782.4:2000SEP0885541555 5059 5361
143 LI;198782.4:2000SEP087734334H2 5133 5354
143 LI:198782.4:2000SEP088867243 5123 5328
143 LI:198782.4:2000SEP088788934 5130 5351
143 LI;198782.4:2000SEP084515811 H 5130 5335
1
T 43 LI;198782.4:2000SEP082581176H 1 5147 5355
143 LI:198782.4:2000SEP0881192667 5201 5355
143 LI:198782.4:2000SEP088783039 5202 5354
143 ~ LI;198782.4:2000SEP0881481930 5240 ~ 5356
143 LI:198782.4:2000SEP088.6567865 5252 5354
143 LI:198782.4;2000SEP082129634H1 5300 5354
144 LI;2030279.1:2000SEP0884124200 1540 1885
144 LI:2030279.1;2000SEP0885664544 1561 1885
144 LI;2030279.1:2000SEP0885678269 1426 1885
144 LI:2030279.1:2000SEP084097504F8 121 b 1756
144 LI;2030279.1:2000SEP084097583H1 1206 1368
144 LI;2030279.1:2000SEP088819125 993 1354
144 LI;2030279.1;2000SEP088847513 998 1344
144 LI:2030279.1;2000SEP088894561 1133 1326
144 LI:2030279.1:2000SEP08239737076 1127 1322
144 LI;2030279.1:2000SEP088847512 711 1034
144 LI:2030279.1;2000SEP088894605 7 i 1 972
144 LI:2030279. 7 8819124 711 949
;2000SEP08
144 LI:2030279.1:2000SEP088969809 414 815
144 LI:2030279.1;2000SEP084958864H1 676 810
144 LI:2030279.1:2000SEP087702090H1 686 767
144 LI:2030279.1;2000SEP082397370H1 664 767
144 LI;2030279.1:2000SEP082397370F6 664 767
144 LI:2030279.1;2000SEP083048139H 1 658 767
144 LI:2030279.1:2000SEP088888757 395 681
144 LI:2030279.1:2000SEP088734030 136 566
144 LI;2030279.1;2000SEP088791917 i 81 465
144 LI:2030279.1:2000SEP08g 1013278 223 447
144 LI:2030279.1;2000SEP088838950 1 336
181
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
144 LI:2030279,1:2000SEP08g6989913 T 648 1887
144 LI:2030279.1:2000SEP08g3752364 1454 1886
145 LI:1 Ol 8424.3:2000SEP0871223752V 1083 1652
1
145 LI:1018424.3:2000SEP085781891 F6 685 1177
145 LI:1018424.3:2000SEP085781891 H1 685 934
145 LI:1018424.3:2000SEP0871222903V1 685 1288
145 LI:1018424,3:2000SEP083719168H1 933 1218
145 LI:1018424,3:2000SEP0837191 i58Fb 933 1403
145 LI:1018424.3:2000SEP0871223803V1 1083 1500
145 LI:1 Ol 8424.3:2000SEP0871223901 676 1359
V 1
i 45 LI:1018424.3:2000SEP087694804) 276 646
1
145 LI:1018424,3:2000SEP087639804H2 391 989
145 LI:10~18424,3:2000SEP087711179H1 474 935
145 LI:1018424.3:2000SEP085946909H1 480 802
145 LI:1018424,3:2000SEP086857771H1 537 1017
145 LI:1018424.3:2000SEP087383248H 64 468
1
145 LI:1018424.3:2000SEP087639804)2 b9 684
145 LI:1018424,3:2000SEP087226703H1 156 662
145 LI: T O 18424.3:2000SEP087222639H 1 482
1
145 LI:1018424.3:2000SEP0870842868V1 1118 1795
i 46 Ll: 7 30969,1:2000SEP084344164H 1895 1989
1
146 LI:130969.1:2000SEP086470643H1 1967 2601
146 LI:130969.1:2000SEP087720888) 2002 2357
1
146 LI:130969,1:2000SEP087579533H 2179 2762
1
146 LI:1309b9.1:2000SEP084874203H 2227 2497
1
146 LI:1309b9,1:2000SEP081506715H 2251 2520
1
146 LI:130969.1:2000SEP085334709F8 2355 2918
146 LI:130969.1:2000SEP085334709F6 2355 2643
146 LI:130969,1:2000SEP08524905H 1 467 716
146 LI:130969.1:2000SEP081871804H1 196 450
146 LI:1309b9.1:20DOSEP083661732F8 334 916
146 LI:130969,1:2000SEP087725180) 303 866
1
146 LI:130969.1:2000SEP08526216H 1 466 714
146 LI:130969,1:2000SEP083661732T8 1 487
146 LI:130969,1:2000SEP08g5544854 153 315
146 LI:1309b9.1:2000SEP087725592H1 720 1367
146 LI:130969,1:2000SEP087318481 H 683 1244
1
i 46 LI:130969.1:2000SEP086357 i 21 659 800
H 1
146 LI:130969.1:2000SEP087660892) 625 1053
1
146 LI:130969.1:2000SEP083661732H1 633 916
146 LI:130969,1:2000SEP081657839H 583 694
1
146 LI:130969.1:2000SEP087000424F8 620 1198
146 LI:130969.1:2000SEP08700042488 620 ~ 1198
146 LI:130969.1:2000SEP083788096H 921 1040
1
146 LI:130969.1:2000SEP087720888H 1092 1679
1
146 LI:130969,1:2000SEP087000424H 1137 1198
1
146 LI:130969.1:2000SEP088034483) 1261 1800
1
146 LI:130969.1:2000SEP087718653) 1555 2210
1
182
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
146 LI:1309b9.1;2000SEP087986828H1 ' 1661 2270
146 LI:130969.1:2000SEP087660868H1 1881 2490
146 LI:130969.1:2000SEP083460547H1 2469 2660
146 LI:1309b9.1:2000SEP085334709H 1 2355 2553
146 LI:1309b9.1;2000SEP084874203F7 2426 2760
146 LI:130969.1:2000SEP087726534H 1 737 1285
147 LI;286246.2;2000SEP0871537829V1 735 1233
147 LI:286246.2;2000SEP0870562337V1 1821 2410
147 LI:28b246.2:2000SEP0870843114V1 1565 1808
147 LI;286246.2;2000SEP0871553959V1 2070 2421
147 LI;286246.2:2000SEP0871670404V1 1621 1920
147 LI:28b246.2:2000SEP086152650H 1 2579 2710
147 LI:286246.2:2000SEP081941561H1 2459 2705
147 LI;286246.2:2000SEP0871539112V1 2393 2710
147 LI:28b246.2:2000SEP08003298H 1 2088 2574
1
147 LI:286246.2:2000SEP0871539117V1 943 1334
147 LI;28b246.2:2000SEP0871045845V1 744 1329
147 LI;28b246.2:2000SEP0871542189V1 1171 1330
147 LI;286246.2;2000SEP0871540655V1 1503 1889
147 LI;28b246.2:2000SEP08753064H1 207 446
147 LI;28b246.2:2000SEP0871554818V1 207 383
147 LI;286246.2;2000SEP084835102H1 70 336
147 LI:286246.2;2000SEP0871543193V1 1373 1761
147 LI:286246.2:2000SEP0871243325V1 1621 1745
147 LI:28b246.2:2000SEP08~ 71540358V1 1706 2207
147 LI:286246.2;2000SEP0870555497V1 1585 2205
147 LI:286246.2:2000SEP0870561143V1 1554 2200
147 LI:28b246.2;2000SEP0871223039V1 1822 1907
147 LI;286246.2;2000SEP0871047689V1 1555 1882
147 LI;286246.2;2000SEP0870561457V1 1427 1720
147 LI;286246.2:2000SEP0871047089V1 1228 1721
147 LI:286246.2;2000SEP0871537608V1 1390 1702
147 LI;286246.2;2000SEP0871246060V1 1629 1702
147 LI;28b246.2;2000SEP0870560146V1 1189 1696
147 LI:28b246.2:2000SEP0871539205V1 1022 1693
147 LI:286246.2;2000SEP0871538958V1 2000 2460
147 LI:28b246.2:2000SEP0870560255V1 1725 1868
147 LI:28b246.2;2000SEP0870561356V1 1408 1867
147 LI:286246.2:2000SEP0871537611V1 1540 1982
147 LI:286246.2;2000SEP0870561842V1 1369 1979
147 LI:28b246.2:2000SEP0871048263V 1221 1950
1
147 LI:28b246.2;2000SEP0871539434V1 1382 2023
147 LI:28b246.2;2000SEP087123394H1 214 510
147 LI:28b246.2;2000SEP0871045783V1 744 1431
147 LI:286246.2:2000SEP0871051139V1 1724 1913
147 LI;286246.2;2000SEP0871048786V1 1675 2130
147 LI:286246.2:2000SEP0871048066V1 1546 2112
147 LI:28b246.2;2000SEP0870561666V1 1586 2142
IgJ
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
147 LI;286246.2;2000SEP0870560120V1 1459 2096
147 LI:286246.2;2000SEP086274047H1 1495 2090
147 , LI:286246.2;2000SEP0871243756V1 1484 2083
147 LI;286246.2:2000SEP0870561769V1 1533 2141
147 LI:286246.2:2000SEP0871242738V1 1454 2082
147 LI:286246.2;2000SEP0871046929V1 1546 2082
147 LI:286246.2;2000SEP0871243123V1 1443 2078
147 LI:286246.2:2000SEP0871046075V1 1371 2078
147 LI:28b246.2;2000SEP0871054743V1 1868 2071
147 LI:286246.2;2000SEP0871540043V1 1860 2061
147 LI:28b246.2:2000SEP0871540268V1 1327 2060
147 LI:28b246.2;2000SEP0870560467V1 1377 2056
147 LI;28b246.2;2000SEP0871245530V1 2527 2710
147 LI:286246.2:2000SEP0871539404V1 2136 2589
147 LI;28b246.2;2000SEP0871539423V1 2109 2598
147 LI:286246.2;2000SEP08500290279 1992 2587
147 LI:28b246.2:2000SEP0871045716V1 2154 2520
147 LI:28b246.2;2000SEP0871540558V1 860 1321
147 LI:286246.2:2000SEP0871047312V1 2085 2710
147 LI:28b246.2;2000SEP086206414H1 2071 2710
147 LI:28b246.2;2000SEP0871553195V1 1028 1389
147 LI:286246.2:2000SEP0871538629V1 1373 1761
147 LI:28b246.2:2000SEP0871542943V1 1319 1759
147 LI:286246.2;2000SEP0871540253V1 792 1532
147 LI;286246.2;2000SEP0871045637V1 1129 1490
147 LI:28b246.2:2000SEP086271029H2 965 1523
147 LI:28b246.2:2000SEP0870555132V1 922 1520
147 LI:28b246.2;2000SEP0871542566V1 937 1475
147 LI:28b246.2:2000SEP0871242763V1 744 1465
147 LI;286246.2;2000SEP0871244412V1 1129 1454
147 LI:28b246.2:2000SEP0871544889V1 978 1451
147 LI:28b246.2;2000SEP0871538305V1 1111 1452
147 LI:28b246.2:2000SEP0871540285V1 1147 1592
147 LI:286246.2:2000SEP0871540983V1 1083 1593
147 LI:286246.2;2000SEP0871542737V1 1036 1565
147 LI:28b246.2;2000SEP086743680Ni 10i6 1567
147 LI:28b246.2;2000SEP0870559871 Vl 1258 1644
147 LI:28b246.2;2000SEP087123394F8 219 510
147 LI:286246.2:2000SEP087585860H2 1 633
147 LI;286246.2:2000SEP0871244615V1 1676 1872
147 LI:28b246.2;2000SEP0871540204V1 .1382 1632
147 LI:286246.2:2000SEP084122893H1 1970 2202
147 LI;286246.2;2000SEP0871046145V1 1579 2193
147 LI:28b246.2:2000SEP0871052465V1 1753 1913
147 LI;286246.2;2000SEP0871541476V1 1379 1920
147 LI:286246.2:2000SEP0871040490V1 1135 1220
147 LI:286246.2:2000SEP085680372F6 744 1136
147 LI:28b246.2:2000SEP0871545686V1 1545 1883
184
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component ' Start Stop
NO: ID
147 LI:286246,2:2000SEP0871244339V1 1203 1881
147 LI:286246,2:2000SEP08194156176 2455 267i
147 LI:286246.2:2000SEP0870843645V1 1609 2162
147 LI:28b246,2:2000SEP0871047111V1 1484 2145
147 LI:28b246,2:2000SEP0871244003V1 1235 1796
147 LI:286246.2:2000SEP08g672529 1426 1778
147 LI:28b246,2:2000SEP0870559901 V 1225 1773
1
147 LI:286246,2:2000SEP0871044921 V1 1336 1761
147 LI:286246.2:2000SEP0871047834V1 2048 2689
147 LI:28b246,2:2000SEP0871045886V1 1259 1848
147 LI:286246,2:2000SEP0871048727V1 1512 1832
147 LI:286246.2:2000SEP0871543038V1 7341 1839
147 LI:286246,2:2000SEP0871048663V1 1283 1821
147 LI:286246,2:2000SEP0870560339V1 1148 1819
147 LI:28b246.2:2000SEP0870561925V1 1988 2388
147 LI:28b246,2:2000SEP0871047478V1 1343 1864
147 LI:286246.2:2000SEP0871539466V1 1138 1866
147 LI:286246,2:2000SEP0871545826V1 1471 1687
147 LI:28b246,2:2000SEP0871540331 V1 931 1667
147 LI:28b246.2:2000SEP0871044926V1 1191 1666
147 LI:28b246,2:2000SEP0871540192V1 1155 1657
147 LI:28b246,2:2000SEP0871242808V1 2120 2727
147 LI:286246.2:2000SEP0871047724V1 2122 2729
147 LI:286246.2:2000SEP0871047489V1 2234 2712
147 LI:28b246,2:2000SEP0871047868V1 2067 2727
147 LI:286246.2:2000SEP0870449628V1 782 1120
147 ~ LI:28b246,2:2000SEP08500290278 1961 2586
147 LI:286246.2:2000SEP08g2410095 2232 2356
147 LI:28b246.2:2000SEP0870555842V1 1640 2314
147 LI:286246.2:2000SEP0871243779V1 1729 2294
147 LI:286246.2:2000SEP0871047027V1 1654 2270
147 L1:286246.2:2000SEP0871048396V1 1229 1882
147 LI:286246,2:2000SEP0871054331 Vl 1388 1889
147 LI:28b246.2:2000SEP0871542747V1 807 1290
147 LI:28b246.2;2000SEP0871542294V1 564 1304
147 LI:28b246,2:2000SEP0871542526V1 535 1289
147 LI:286246.2:2000SEP087 i 538262Vi 973 1289
147 LI:286246,2:2000SEP08g2880816 2281 2711
147 LI:28b246.2:2000SEP0871244177V1 2001 2710
147 LI:286246.2:2000SEP0871243630V1 2085 2710
147 LI:286246.2:2000SEP087192911 H2 2144 2715
147 LI:28b246,2:2000SEP085957753H1 927 1541
147 LI:286246.2:2000SEP0871539504V1 1039 1564
147 LI:28b246.2:2000SEP0871045343V1 1326 1723
147 LI:28b246,2:2000SEP0871044936V1 1104 1722
147 LI:28b246.2:2000SEP0871537853V1 1830 2259
147 LI:28b246,2:2000SEP0870560372V1 1957 2257
147 LI:28b246.2:2000SEP0871042212V1 1897 2252
_ .
1g5
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
147 LI:28b24b.2:2000SEP0871552852V1 1911 2248
147 LI:28b24b,2:2000SEP0871539575V1 1942 2229
147 LI:28b246,2:2000SEP0871541594V1 411 ~ 793
147 LI:28b24b.2:2000SEP0871541763V1 411 793
147 LI:28b24b.2:2000SEP0870560200V1 207 660
147 LI:286246,2:2000SEP087585591 H 87 640
1
147 LI:28624b.2:2000SEP0871046729V1 1219 1956
147 LI:286246.2:2000SEP0871242804V1 1268 1949
147 LI:28624b,2:2000SEP0871244132V1 1265 1949
147 LI:28b246.2:2000SEP0871046686V1 2270 2407
147 LI:28b246,2:2000SEP0871538241 803 1223
V1
147 LI:28b24b,2:2000SEP0871537787V1 1111 1688
147 LI:28624b.2:2000SEP0871545679V1 1471 1687
147 LI:286246,2:2000SEP0870562308V1 767 1425
147 LI:28b246,2:2000SEP0871541788V1 1752 2513
147 LI:28b246.2:2000SEP0870560053V1 2358 2528
147 LI:28b24b,2:2000SEP0871538135V1 1498 2226
147 LI:28b24b,2:2000SEP0871540782V1 1530 2226
147 LI:28b246.2:2000SEP0870561243V1 1999 2227
147 LI:28b24b,2:2000SEP0871047521V1 1324 1944
147 LI:28b24b,2:2000SEP0871048819V1 1540 1946
147 LI:286246.2:2000SEP0870560938V 1413 1933
1
147 LI:28b246,2:2000SEP0871046883V1 1862 1932
147 LI:28b24b,2:2000SEP0870560136V1 2015 2169
147 LI:286246.2:2000SEP0870559818V1 2016 2169
147 LI:28624b,2:2000SEP0871542272V1 1668 2172
147 LI:28b24b,2:2000SEP08g566024 2437 2710
147 LI:28b24b,2:2000SEP0871538083V1 627 1249
147 LI:28b246,2:2000SEP0870560000V1 2303 2508
147 LI:286246,2:2000SEP0871048026V1 1793 2518
147 LI:286246.2:2000SEP0871541892V1 1094 1688
147 LI:28624b,2:2000SEP0871245539V1 1441 1686
147 LI:28b24b.2:2000SEP0871048644V1 2061 2468
147 LI:286246,2:2000SEP0871539253V1 746 1262
147 LI:28624b.2:2000SEP087235757H1 1967 2515
147 LI:286246.2:2000SEP0871243486V1 2071 2691
147 LI:28b24b,2:2000SEP0871540750V1 832 1421
147 LI:28b24b,2:2000SEP0871045227V1 1769 2476
147 LI:286246.2:2000SEP087188311 H2 618 1094
147 LI:28624b,2:2000SEP0871044942V1 735 1068
147 LI:286246.2:2000SEP0871541024V1 670 1070
147 LI:28624b,2:2000SEP085680372H1 744 1033
147 L1:286246.2:2000SEP0871548384V 735 1 Ol
1 8
147 LI:286246.2:2000SEP0871541347V1 559 1017
147 LI:28b24b.2:2000SEP0871546203V1 798 919
147 LI:28b24b,2:2000SEP0871243338V1 1140 1750
147 LI:28b24b,2:2000SEP0870562153V1 1102 1744
147 LI:28624b.2:2000SEP0871045853V1. 2136 2366
186
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SECT Template ID Component Start Stop
ID NO: ID
147 LI:28b246.2;2000SEP0871553295V1 1028 1389
147 LI;286246.2:2000SEP0871553334V1 1028 1389
147 LI:28b246.2;2000SEP0871543267V1 1026 1352
147 LI;286246.2:2000SEP0871541778V1 2535 2710
147 LI:286246.2;2000SEP08i941561Rb 2455 2710
147 LI:28b246.2:2000SEP086340283H 1 2219 2710
147 LI:28b246.2:2000SEP08g6991789 2229 2710
147 LI:286246.2:2000SEP0871541225V1 2234 2710
147 LI:286246.2:2000SEP0871547092V1 2240 2366
147 LI;286246.2;2000SEP0871540847V1 2178 2384
147 LI;286246.2:2000SEP0871048693V1 1989 2700
147 LI:28b246.2;2000SEP0871541749V1 735 1261
147 LI:286246.2;2000SEP0871542963V1 1274 1834
147 LI;286246.2:2000SEP0871538861 V1 1834 2598
i47 LI:28b246.2;2000SEP087i539143Vi 1976 2460
147 LI;286246.2:2000SEP0871553879V1 2141 2478
147 LI:28b246.2:2000SEP0871044960V1 1787 2460
148 LI:001527.1:2000SEP08g5877753 987 1301
148 LI:001527.1;2000SEP08g6657300 1129 1301
148 LI:001527.1:2000SEP086341247H1 908 1301
148 LI;001527.1:2000SEP083409462F6 910 1301
148 LI:001527.1:2000SEP083409462H 1 910 11 b4
148 LI;001527.1:2000SEP0871548338V1 151 847
148 LI:001527.1;2000SEP0871548312V1 154 817
148 LI:001527.1:2000SEP084698934H1 504 763
148 LI:001527.1:2000SEP087735666H i 506 1 i
O 1
148 LI;001527.1:2000SEP087691689H2 679 1235
148 LI:001527.1;2000SEP08g3961333 791 1162
148 LI:001527.1;2000SEP08798740H1 1 246
148 LI:001527.1;2000SEP084041891 H 13 309
1
148 LI:001527.1:2000SEP086778992H1 15 534
148 LI:001527.1:2000SEP0871549018V 36 631
1
148 LI:001527.1:2000SEP083044046F6 36 406
148 LI:001527.1:2000SEP083044046H1 36 286
148 LI:001527.1;2000SEP087925481 H 36 179
1
148 LI:001527.1:2000SEP086778992F8 60 637
148 LI:001527.1:2000SEP08g2063121 60 410
149 LI;395063.1:2000SEP087093763H1 1378 1781
149 LI:395063.1;2000SEP087093324F8 1378 1781
149 LI;395063.1:2000SEP087093763F8 1398 1781
149 LI:395063.1;2000SEP0870678552V1 1438 2018
i 49 LI;395063.1:2000SEP0870669601 V i 453 T 679
i
149 LI;395063.1:2000SEP0870672059V1 1296 1514
149 LI:3950b3.1:2000SEP0870647192V1 1368 2058
149 LI:3950b3.1;2000SEP0870676346V1 1375 2026
149 LI:3950b3.1:2000SEP08573252678 1151 1651
149 LI;3950b3.1:2000SEP081426382H1 1240 1516
149 LI:3950b3.1:2000SEP085732526H1 1047 1322
187
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID ~ Component Start Stop
NO: ID
149 LI:3950b3.1:2000SEP085732526F8 1047 1701
149 LI:395063.1:2000SEP0870674516V1 1460 2065
149 LI;395063.1:2000SEP0870679043V1 1630 2200
149 LI:395063.1;2000SEP087401964H1 1662 2218
149 LI:395063.1:2000SEP0870674896V1 1687 2089
149 LI:395063.1;2000SEP0870671138V1 1742 2263
149 LI;395063.1:2000SEP084445453H1 599 868
149 LI:395063.1:2000SEP083486280H 1 1573 1669
149 LI;395063.1:2000SEP0870672954V1 1587 1842
149 LI:395063,1:2000SEP083859309H 1 318 405
149 LI:395063.1;2000SEP083859388H 1 379 454
149 LI:3950b3.1:2000SEP0870671032V1 1283 1608
149 LI:395063.1;2000SEP0870678302V1 1909 2233
149 LI:395063.1:2000SEP082674222H 1 1896 2131
149 LI:3950b3.1:2000SEP083696546F6 810 1323
149 LI;395063.1:2000SEP083696546H1 812 1096
149 LI:3950b3.1;2000SEP0814707086 384 585
149 LI:3950b3.1;2000SEP08g5361477 373 759
149 LI:3950b3.1;2000SEP08147070H 1 354 534
149 LI;3950b3.1:2000SEP087700096H1 254 855
149 LI;395063.1;2000SEP08030545H1 141 365
149 LI;395063.1;2000SEP084180711 H 54 201
1
149 LI:3950b3.1:2000SEP086828352) 1 67 594
149 LI:395063.1;2000SEP087610103H1 75 603
149 LI;3950b3.1;2000SEP086788461 H1 40 474
149 LI:395063.1;2000SEP087086817H 1 402 950
149 LI;395063.1:2000SEP08g3596902 494 933
149 LI:3950b3.1:2000SEP08g67,02152 1545 1738
149 LI;395063.1:2000SEP0870675746V1 1569 2106
149 LI;3950b3.1:2000SEP0870676091V1 1569 2170
149 LI;395063.1:2000SEP0870674954V1 1545 2135
149 LI;395063.1;2000SEP086828352H1 619 1165
149 LI:3950b3.1:2000SEP084445453F8 624 1198
149 LI;395063.1:2000SEP083696546T6 1926 2487
149 LI:395063.1;2000SEP0870677175V1 2017 2405
149 LI:395063.1;2000SEP08g4898324 445 712
149 LI:395063.1:2000SEP08g3424526 540 768
149 LI;395063. i :2000SEP08g867186 558 830
149 LI;395063.1;2000SEP083699565H1 1 285
149 LI;395063.1:2000SEP086785022H1 36 340
149 LI:395063.1;.2000SEP0803 T 180H 206 365
1
149 LI;395063.1:2000SEP085862502H 1 273 559
149 LI:395063.1;2000SEP08g3181305 2275 2520
149 LI;395063.1:2000SEP084668004H1 2453 2552
149 LI:3950b3.1;2000SEP082866136H 1 2024 2272
149 LI:395063.1:2000SEP08g5054669 2151 2520
149 . LI:395063.1;2000SEP083535582F6 2663 3209
149 LI:3950b3.1:2000SEP083535582H1 2663 2938
_.__ _ -_- -
-
1SS
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
T 49 LI:3950b3.1:2000SEP084668484H 1 2698 2946
149 LI:395063.1:2000SEP084073057H 1 2716 3005
149 LI:3950b3.1:2000SEP084073057F6 2715 2783
149 LI:3950b3.1;2000SEP08g3849737 2740 3025
149 LI;3950b3.1;2000SEP0870667545V1 1457 1594
149 LI;395063.1:2000SEP0870671598V1 1456 1799
149 LI:395063.1;2000SEP0870677802V1 1461 1655
149 LI;3950b3.1;2000SEP085455621 H1 740 986
149 LI;3950b3.1:2000SEP083484210H1 2503 2836
150 LI:1064460.1:2000SEP087088717H1 771 844
150 LI;1064460.1;2000SEP087088717F8 771 844
150 LI:1064460.1:2000SEP0871249237V1 290 833
150 Li;1064460.1;2000SEP0871249652V1 135 581
150 LI:lOb4460.1:2000SEP085872722F8 6 213
150 LI:1064460.1;2000SEP085872722H 1 1 176
150 LI:1064460. T 71 Ob501 OV 19 457
:2000SEP08 1
150 LI: l Ob44b0.1;2000SEP08708871788 783 879
150 LI:lOb4460.1:2000SEP0871062996V7 597 866
150 LI;10644b0.1:2000SEP08587272278 600 844
151 LI;344b90.2:2000SEP08g3278472 609 992
151 LI;344690,2;2000SEP08g2838775 609 919
151 LI:344690.2:2000SEP08614291778 388 846
151 LI:344b90.2:2000SEP086142917H1 1 313
151 LI:344690.2:2000SEP086142917F8 1 609
151 LI:344690.2;2000SEP08549530386 275 526
152 LI;061585.4:2000SEP086517628H1 548 1125
152 LI;061585.4:2000SEP086517628F8 571 1125
152 LI:061585.4;2000SEP082402474H1 945 1022
152 LI:0b1585.4:2000SEP082707794H1 519 827
152 LI:061585.4;2000SEP086756967J 1 54 692
152 LI:061585.4;2000SEP086757164J1 45 687
152 LI:Obl585.4:2000SEP08675716488 159 674
152 LI:Ob1585.4:2000SEP08g920342 364 639
152 Li;061585.4:2000SEP085215067H 1 1 254
153 LI:378428.1:2000SEP08681654388 1 646
153 ~ LI:378428.1:2000SEP087379418H1 36 610
153 LI:378428.1:2000SEP087379444H 1 36 600
153 LI;378428.1;2000SEP086816543H1 185 642
153 LI:378428.1;2000SEP086816543F8 285 643
153 LI:378428.1;2000SEP08742939 T H 439 964
1
153 LI:378428.1:2000SEP087273611 H1 550 1010
153 LI;378428.1;2000SEP086910888F8 561 712
T 53 LI:378428.1:2000SEP08440130576 2044 2406
153 LI:378428.1;2000SEP08g2006832 2145 2450
153 LI:378428.1:2000SEP087010551 H1 1657 2033
T 53 LI:378428.1;2000SEP08g6713687 2001 2431
153 LI:378428.1;2000SEP08g2912647 2015 2434
153 LI:378428.1:2000SEP08g2789298 2045 2440
189
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
153 LI:378428,1:2000SEP086755205H 1 599 1209
153 LI:378428.1:2000SEP086044422F8 729 1350
153 LI:378428,1:2000SEP086044422N 1 730 1031
153 LI:378428.1:2000SEP086910888) 1 772 1385
153 LI:378428.1:2000SEP08691088888 777 1458
153 LI:378428,1:2000SEP085045827F6 1037 1515
153 LI:378428.1:2000SEP085045827H1 1037 1314
153 LI:378428.1:2000SEP087004756H 1 1 Ob8 1508
153 LI:378428,1:2000SEP084401305F6 1240 1776
153 LI:378428,1:2000SEP084401305H 1 i 240 1495
153 LI:378428,1:2000SEP08604442288 1312 1909
153 LI:378428.1:2000SEP086044422) 1 1427 1909
153 LI:378428.1:2000SEP08549711388 1566 2121
153 LI:378428,1:2000SEP08549711389 1574 2121
153 LI:378428.1:2000SEP085497113H1 1574 1852
153 LI:378428.1:2000SEP086910888H 1 561 1116
'
i53 LI:378428,1:2000SEP085497113F9 1580 2121
154 LI:474108.2:2000SEP08g2835390 776 1001
154 LI:474108.2:2000SEP083529569F6 888 1348
154 LI:474108.2:2000SEP083529569H1 888 1186
154 LI:474108.2:2000SEP086476970H 1 1087 1614
154 LI:474108,2:2000SEP083966528H1 4444 4695
154 LI:474108.2:2000SEP082409325H 1 2467 2695
i 54 LI:474108,2:2000SEP085370460H 1 2490 2742
154 LI:474108.2:2000SEP083966735H1 4444 4655
154 LI:474108,2:2000SEP085999741 H 653 1131
1
154 LI:474108,2:2000SEP086076866H1 691 993
154 LI:474108.2:2000SEP087946383H1 330 1038
154 LI:474108,2:2000SEP087946383)2 380 996
154 LI;474108,2:2000SEP086312090H1 620 1172
154 LI:474108.2:2000SEP084610508H1 2681 2914
154 LI:474108,2:2000SEP085770375H1 2684 2762
154 LI:474108.2:2000SEP084706706H 1 2691 2860
154 LI:474108,2:2000SEP082858550H1 2732 3020
154 LI:474108,2:2000SEP0870413228D1 2467 2953
154 LI:474108.2:2000SEP082289904H1 4219 4373
154 LI:474108.2:2000SEP08g 1189353 4229 4414
154 LI:474108.2:2000SEP084720055H 1 4239 4420
154 LI:474108,2:2000SEP086441390H1 4253 4616
154 LI:474108,2:2000SEP084312223H1 4299 4591
154 LI:474108,2:2000SEP082289904Th 4219 4680
154 LI:474108.2:2000SEP08588846H 1 4218 4376
154 LI:474108,2:2000SEP08228990486 4219 4599
154 LI:474108.2:2000SEP082284264H 1 4702 4977
154 LI:474 i 08.2:2000SEP0870645 i 4H 4755 4858
1
154 LI:474108.2:2000SEP08g4305681 4158 4633
154 LI:474108.2:2000SEP08758041 OH 3714 4273
1
154 LI:474108.2:2000SEP081730089F6 3714 4203
190
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
154 LI:474108.2:2000SEP081730089H 1 3714 3927
154 LI:474108.2;2000SEP082863355H1 3735 3986
154 LI:474108.2:2000SEP084017503H1 3741 3932
154 LI:474108.2:2000SEP081459195H 1 3743 3984
154 LI:474108.2:2000SEP084313035H1 3425 3712
154 LI;474108.2:2000SEP083279026H 1 1983 2231
154 LI:474108.2:2000SEP08g4686653 4055 4419
154 LI;474108.2:2000SEP081834781 Rb 4060 4412
154 LI:474108.2:2000SEP083919109H 1 3913 4207
154 LI;474108.2;2000SEP082042616H1 3976 4226
154 LI:474108.2;2000SEP085906142H1 5010 5320
154 LI;474108.2:2000SEP08g5848670 5320 5666
154 LI:474108.2:2000SEP08g3798256 5326 5658
154 LI;474108.2:2000SEP084177811H1 4844 5104
154 LI;474108.2:2000SEP085679828H 1 4623 4862
154 LI:474108.2:2000SEP085021705H 1 2137 2404
154 LI;474108.2;2000SEP086745632H1 2176 2800
154 LI:474108.2;2000SEP088087586H1 2299 2906
154 LI:474108.2;2000SEP086438814H1 3601 4119
154 LI:474108.2:2000SEP08g 1858435 3602 3709
154 LI;474108.2;2000SEP08g1669982 3604 4073
154 LI:474108.2:2000SEP086709569H1 3607 4142
154 LI:474108.2;2000SEP08953289T1 3645 3909
154 LI:474108.2:2000SEP08953289H 1 3655 3941
154 LI;474108.2;2000SEP08g4078055 3436 3861
154 LI:474108.2;2000SEP081455911 H 3445 3706
1
154 LI;474108.2:2000SEP081455911 Fb 3446 3705
154 LI:474108.2:2000SEP08g5632830 3504 3954
154 LI:474108.2:2000SEP08g5663809 3524 3887
154 LI:474108.2:2000SEP08145911681 4048 4414
154 LI:474108.2;2000SEP081700656T6 4052 4576
154 LI:474108.2;2000SEP084062343H1 4035 4243
154 ' LI:474108.2:2000SEP082792204F6 1771 2115
154 LI:474108.2;2000SEP082792204H1 1771 2067
154 LI;474108.2:2000SEP081798559H1 1771 2021
154 LI:474108.2:2000SEP082790426H2 1772 2062
154 LI;474108.2;2000SEP087218380H1 1790 2335
154 LI:474108.2:2000SEP086950195H1 1882 2325
154 LI:474108.2;2000SEP084069262F6 1949 2530
154 LI:474 i 08.2:2000SEP084069262H 1 1949 2243
154 LI:474108.2:2000SEP081834781 H 4060 4288
1
154 LI:474108.2;2000SEP085018950H1 4063 4339
.
154 LI:474108.2:2000SEP083013662H 1 4065 4373
154 LI:474108.2:2000SEP083721022H1 4066 4296
154 LI:474108.2:2000SEP08g6197475 2629 3079
154 LI:474108.2;2000SEP084020928H1 4144 4560
154 LI:474108.2:2000SEP08g5111248 4153 4615
154 LI:474108.2:2000SEP08_5167817H1 2818 3091
- -
191
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SE6~ Template ID Component Start Stop
ID NO: ID
154 LI;474108.2:2000SEP08g3049489 3403 3810
154 LI;474108.2:2000SEP083529569Th 3379 3910
154 LI:474108,2:2000SEP082314219H1 2910 3140
154 LI:474108,2:2000SEP084465916H1 2962 3218
154 LI;474108.2:2000SEP082910754F6 3000 3335
154 LI;474108.2;2000SEP082910754H1 3000 3113
154 LI:474108,2:2000SEP08g1955179 3027 3359
154 LI;474108.2:2000SEP085997172H1 4321 4414
154 LI:474108,2;2000SEP083439856H1 3875 4129
154 LI;474108,2;2000SEP08g3050270 3883 3979
154 LI:474108.2:2000SEP083335693H1 3851 4128
154 LI:474108.2:2000SEP08g2254315 3854 4005
154 LI;474108,2;2000SEP08g3700678 4074 4414
154 LI:474108.2:2000SEP083468642H1 4092 4343
154 LI:474108,2;2000SEP086853588H1 4102 4598
154 LI;474108.2;2000SEP085679472H1 4119 4379
154 LI:474108.2:2000SEP085697968H1 4126 4414
154 LI;474108.2;2000SEP08g5755612 4455 4867
154 LI;474108.2:2000SEP082910754Th 4566 4822
154 LI;474108,2:2000SEP083317408Th 4571 4823
154 LI:474108.2;2000SEP085122440H1 4580 4831
154 LI:474108,2;2000SEP082326892H1 4580 4703
154 LI;474108.2:2000SEP086707429H1 4349 4616
154 LI;474108.2:2000SEP08g2238324 3810 4005
154 LI;474108.2:2000SEP083529938H1 3837 4120
154 LI:474108.2:2000SEP081619057Th 4167 4678
154 LI;474108,2;2000SEP08g1669983 4172 4414
154 LI:474108.2:2000SEP085689137H 1 3376 3651
154 LI;474108,2:2000SEP084177463H1 3040 3279
154 LI;474108,2:2000SEP08g1985965 3041 3140
154 LI:474108,2;2000SEP082838117H2 3043 3307
154 LI:474108,2:2000SEP084460047H1 3086 3331
154 LI;474108.2:2000SEP084755529H1 3138 3315
154 LI;474108.2;2000SEP084069262T6 3142 3740
154 LI;474108.2:2000SEP084032103H1 3170 3427
154 LI:474108.2;2000SEP082847451H1 3265 3533
154 LI:474108.2:2000SEP08g 1194500 3758 4011
154 LI:474108.2:2000SEP08145911 6H 3744 3898
1
154 LI:474108.2;2000SEP084337945H1 3432 3706
154 LI:474108.2:2000SEP086998557H1 1463 1993
154 LI:474108.2:2000SEP081618603H1 1470 1693
154 LI:474108.2;2000SEP081619057H1 1470 1680
154 LI:474108.2;2000SEP081619057F6 1479 1890
154 LI:474108.2;2000SEP086525893H1 1643 2292
154 LI:474108.2;2000SEP081566052H1 1759 1955
154 LI;474108.2:2000SEP085404641H1 1088 1184
154 LI;474108.2:2000SEP083531652F6 1118 1613
154 LI:474i 08.2;2000SEP083531652H i i 1 T 1444
8
_. _ X92
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
154 LI:474108,2:2000SEP087249758H2 1398 1959
154 LI:474108.2:2000SEP081834781 T6 4060 4377
154 LI:474108,2:2000SEP083713858H1 2615 2730
154 LI:474108.2:2000SEP08g7316893 2626 3079
154 LI:474108.2:2000SEP084711425H1 2494 2750
154 LI:474108,2:2000SEP085331713H1 2528 2793
154 LI:474108.2:2000SEP085181291 H 4642 4870
1
154 LI:474108.2:2000SEP085680433H1 2824 3088
154 LI:474108.2:2000SEP085680401 H 2826 3023
1
154 L1:474108.2:2000SEP086709569J 1 4414 4866
154 LI:474108.2:2000SEP08g5108937 4444 4867
154 LI:474108,2:2000SEP08g1193841 3977 4169
154 LI:474108.2:2000SEP08g3418917 4035 4414
154 LI:474108.2:2000SEP08g3539298 4035 4422
154 LI:474108,2:2000SEP08gi 190912 4653 4862
154 LI:474108.2:2000SEP085369964H 1 4314 4579
154 LI:474108,2:2000SEP085369955H1 4314 4582
154 LI:474108.2:2000SEP08g 1980043 4321 4601
154 LI:474108.2:2000SEP085388940H1 2032 2286
154 LI:474i08.2:2000SEP087087904H1 1 387
154 LI:474108.2:2000SEP083078492H 1 28 314
154 LI:474108.2:2000SEP087984912H1 31 586
154 LI:474108.2:2000SEP08g2220897 313 700
154 LI:474108,2:2000SEP083317408F6 4444 4863
154 LI:474108.2:2000SEP083317408H 1 4444 4693
154 LI:474108,2:2000SEP084177811 F6 4843 5390
155 LI:230711,2:2000SEP0871594734V1 109 686
155 LI:230711.2:2000SEP0871592650V1 1006 1701
155 LI:230711,2:2000SEP0871596872V1 311 1046
155 LI:230711.2:2000SEP0871596548V1 219 775
155 LI:230711.2:2000SEP0871596601 V 277 928
1
155 LI:230711.2:2000SEP0871592649V 296 946
1
155 LI:230711.2;2000SEP0870682403V1 309 861
155 LI:230711,2:2000SEP0870683980V1 309 835
155 LI:230711.2:2000SEP083925359H 1 309 620
155 LI:230711.2:2000SEP083925359F6 309 494
155 LI:230711,2:2000SEP0870682278V1 309 475
155 L1:230711.2:2000SEP08g387128 i i 648 2103
155 LI:230711.2:2000SEP08g5340629 1655 2055
155 LI:230711,2:2000SEP08g5441107 1659 2114
155 LI:230711.2:2000SEP08g6662367 1663 2126
155 LI:230711,2:2000SEP08g2934374 1667 2114
155 LI:230711.2:2000SEP08g7317140 1690 2126
155 LI:230711.2:2000SEP08g5055123 1692 2103
155 LI:230711,2:2000SEP081431377H1 1817 2063
155 LI:230711.2:2000SEP08g3047856 1835 2117
155 LI:230711.2:2000SEP08g4371425 1850 2114
155 LI:230711.2:2000SEP087127851 T8 1872 1932
193
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
155 LI;230711.2:2000SEP0870686041 V 1877 2038
1
155 LI:230711.2;2000SEP08g4327870 1893 2114
155 LI:230711,2:2000SEP08g3134381 1893 2114
155 LI:230711.2:2000SEP0871597167V1 341 1092
155 LI:230711,2;2000SEP083531535H1 364 703
155 LI;230711.2:2000SEP0871591783V1 421 1023
155 LI:230711.2;2000SEP0871596281 V1 440 1017
155 L1:230711.2;2000SEP087125750F8 26 563
155 LI;230711.2:2000SEP086452362F8 1 542
155 LI:230711.2;2000SEP0871591870V1 838 1445
155 L1:230711,2:2000SEP0871592920V1 876 1542
155 LI:230711.2:2000SEP0871591585V1 927 1619
155 LI:230711,2:2000SEP0870680431 V1 466 952
155 LI;230711.2:2000SEP0871594480V1 477 1181
155 LI:230711.2:2000SEP0871597256V1 525 1152
155 LI.230711.2:2000SEP0870683177V1 1071 1594
155 LI:230711.2;2000SEP0871592455V1 463 1113
155 LI:230711,2;2000SEP0871592682V1 806 1413
'
155 LI;230711.2:2000SEP0870708067V1 795 900
155 L1:230711.2:2000SEP0871596835V 824 1530
1
155 LI;230711,2:2000SEP084071965F6 29 403
155 , L1:230711.2:2000SEP0871592539V1 29 ~ 491
155 LI:230711.2;2000SEP084071965H1 29 335
155 L1;230711,2:2000SEP0870680197V1 1076 1454
155 LI:230711,2;2000SEP0871592442V1 1074 1767
155 LI;230711,2:2000SEP0871594276V1 1077 1824
155 LI:230711.2;2000SEP0871592619V1 1082 1771
155 LI;230711,2;2000SEP0871596528V1 1125 1881
155 LI:230711,2:2000SEP0871592914V1 1085 1786
155 LI:230711.2;2000SEP0871621181V1 1136 1668
155 LI;230711,2;2000SEP08~71597169V1 1146 1756
155 LI;230711,2:20bOSEP0871595423V1 1151 1772
155 LI:230711.2;2000SEP0871592214V1 1182 1807
155 LI;230711.2:2000SEP0871592569V1 1103 1855
155 LI:230711.2;2000SEP0871595077V1 1105 1746
155 LI:230711.2;2000SEP0871594784V1 1118 1930
155 LI;230711,2;2000SEP0870679832V1 1205 1830
155 L1;230711.2:2000SEP0870681040V1 1227 1834
155 LI:230711.2:2000SEP087282455H 1 1240 1845
155 LI:230711.2:2000SEP0871593068V1 1259 1927
155 LI;230711.2:2000SEP0870684191V1 1265 1406
155 LI:230711.2:2000SEP0871592819V1 1296 1986
155 LI;230711.2;2000SEP0871593883V1 1295 1956
155 LI:230711.2:2000SEP0871591755V1 1305 2015
155 LI:230711.2;2000SEP0871597148V1 1307 2018
155 LI;230711,2:2000SEP0870687607V1 1325 1749
155 L1:230711.2:2000SEP0871596350V 1330 1607
1
155 LI:230711.2:2000SEP0871597474V1 1345 2092
194
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
155 LI;230711.2;2000SEP0871595132V1 1353 1876
155 LI:230711.2:2000SEP0871593826V1 1357 2068
155 LI:230711.2:2000SEP0870680047V1 1347 2005
155 LI:230711.2:2000SEP0870679818V1 1374 2074
155 LI:230711.2:2000SEP0871595653V1 1385 1998
155 LI:230711.2:2000SEP086543969H 1 1385 1970
155 LI:230711.2:2000SEP084378643F7 1431 2051
155 LI:230711.2;2000SEP084379312F7 1431 1981
155 LI:230711.2:2000SEP084379312H1 1431 1723
155 LI:230711.2:2000SEP084378643H1 1432 1734
155 LI;230711.2;2000SEP0870682506V1 1450 2057
155 LI:230711.2:2000SEP086623832H 1 1450 i 969
155 LI:230711.2;2000SEP0870708640V1 1508 1735
155 LI:230711.2;2000SEP08392535976 1523 2082
155 LI:230711.2;2000SEP08510083979 1546 1866
155 LI:230711.2:2000SEP08437864377 1577 2010
155 LI:230711.2:2000SEP0870682 7 05V 1579 2114
1
155 LI:230711.2:2000SEP08407196576 1600 2121
155 LI:230711.2:2000SEP0871592368V1 1622 2113
155 LI:230711.2:2000SEP08532801276 1625 2076
155 LI;230711.2;2000SEP085328012F6 1632 2035
155 LI:230711.2:2000SEP085328012H1 1632 1889
155 LI:230711.2:2000SEP087127851 F8 35 683
155 LI;23071 i.2:2000SEP087125750H7 27 439
155 LI:230711.2:2000SEP083699779H 1 539 719
155 LI:230711.2;2000SEP0871596494V1 632 1324
155 LI:230711.2:2000SEP0871595289V1 640 1334
155 LI:230711.2:2000SEP0870681329V1 793 964
155 LI:23071 i .2:2000SEP0871593141 V 646 i 271
i
155 LI;230711.2:2000SEP0871594054V1 661 1250
I
155 LI:230711.2:2000SEP0871597118V1 705 1453
155 LI:230711.2;2000SEP0871592473V1 737 1471
155 LI:230711.2:2000SEP0871595446V1 451 1104
155 LI:230711.2:2000SEP0871597018V1 960 1687
155 LI:230711.2:2000SEP0871597402V1 968 1539
155 LI:230711.2:2000SEP0871596514V1 990 1501
i55 LI;230711.2;2000SEP087159i665V1 29 607
156 LI:008942.1:2000SEP086948684F8 1535 1965
156 LI:008942.1:2000SEP086948684H 1 1535 1758
156 LI:008942.1:2000SEP087281566H1 28 439
156 LI;008942.1:2000SEP087952051 H2 25 700
156 LI:008942.1;2000SEP08729055888 39 692
156 LI:008942.1;2000SEP082505107H1 53 157
156 LI:008942.1:2000SEP088116885H1 4 654
156 LI:008942.1;2000SEP087999428H1 38 663
156 LI;008942.1:2000SEP082779147H1 76 348
156 LI:008942.1:2000SEP08g2825239 90 271
156 LI:008942.1:2000SEP087 i 00462H 127 544
1
__ __ - _-
_.
195
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
156 LI:008942.1:2000SEP087997486H1 260 901
156 LI:008942,1:2000SEP086376692H1 1237 1428
156 LI:008942.1:2000SEP0870332069D1 11 b9 1428
156 L1:008942.1:2000SEP0870330927D1 1283 1776
156 LI:008942.1:2000SEP0870309464D 1233 1434
1
156 LI:008942,1:2000SEP0870311281 D1 1283 1798
156 LI:008942,1:2000SEP0870331388D1 1157 1428
156 LI:008942.1:2000SEP0870728082V1 1160 1425
156 LI:008942,1:2000SEP0870310348D1 1358 1803
156 LI:008942,1:2000SEP087966746H 1 1531 2090
156 L1:008942.1:2000SEP082369660H 1 1175 1414
156 LI:008942,1:2000SEP0870330650D1 1231 1426
156 LI:008942,1:2000SEP0870727843V 1175 1427
1
156 LI:008942.1:2000SEP082372577H1 1175 1423
156 LI:008942.1:2000SEP0870729702V1 1175 1414
156 LI:008942,1:2000SEP0870731634V1 1175 1427
156 LI:008942,1:2000SEP0870801702V 1175 1424
1
156 LI:008942.1:2000SEP0870729964V1 1175 1427
156 LI:008942.1:2000SEP082369660F6 1175 1428
156 LI:008942.1:2000SEP082372577F6 1175 1428
156 LI:008942.1:2000SEP087700714H1 1820 2410
156 LI:008942,1:2000SEP08'70311066D1 1158 1427
156 LI:008942.1:2000SEP0870730649V 1168 1428
1
156 LI:008942,1:2000SEP0870310381 D1 1169 1349
156 LI:008942.1:2000SEP0870310610D1 1251 1427
156 LI:008942,1:2000SEP082827340H2 68 362
156 LI:008942.1:2000SEP086918520H1 696 1194
156 Ll:008942,1:2000SEP081423821 Tb 940 1409
156 LI:008942,1:2000SEP087700714) 1 1108 1637
156 LI:008942.1:2000SEP087987293H1 1 572
157 LI:732479. ) :2000SEP086987581 H 1420 1724
1
157 LI:732479.1:2000SEP087006122H1 1551 1748
157 LI:732479.1:2000SEP081314671 Tb 1 360
157 LI:732479,1:2000SEP081314671 F6 8 398
157 LI:732479,1:2000SEP081314671 H 8 235
1
157 LI:732479,1:2000SEP08g1966425 56 531
157 LI:732479,1:2000SEP08g6993126 125 538
157 LI:732479.1:2000SEP08g2063478 170 406
157 LI:732479.1:2000SEP085453575H1 276 518
157 LI:732479.1:2000SEP087015239H 1 397 976
157 LI:732479.1:2000SEP086167048F8 798 1309
157 LI:732479.1:2000SEP083727038H1 530 818
157 LI:732479.1:2000SEP088006617H1 498 1153
157 LI:732479.1:2000SEP083727038F9 530 1069
157 LI:732479,1:2000SEP086167048H 1 980 1310
157 LI:732479.1:2000SEP087077449H 1 1214 1706
157 LI:732479,1:2000SEP081434378H 1 1291 1439
157 LI:732479.1:2000SEP086940364H7 869 1396
I96
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Starf Stop
NO: ID
157 LI:732479.1:2000SEP088112243H1 1 314
157 LI;732479.1:2000SEP087094636H1 789 1287
158 LI;1190250.1:2000SEP086779840H1 9 551
158 LI;1190250.1:2000SEP083386816H 1 1 185
158 LI:1190250.1:2000SEP083888124H 1 4 270
158 LI:1190250.1:2000SEP0870817678V1 294 831
158 LI:1190250,1;2000SEP08195134976 298 805
158 LI:1190250,1;2000SEP086947424H1 304 817
158 LI:1190250.1:2000SEP08g5054158 306 793
158 LI:1190250,1:2000SEP081658838H1 309 541
158 LI:1190250,1:2000SEP0870819393V 180 693
1
158 LI:1190250.1:2000SEP083002522F6 180 469
158 LI:1190250.1:2000SEP0871218340V1 181 693
158 LI:1190250.1;2000SEP083002522H1 181 ~ 492
158 LI:1190250,1:2000SEP0871218644V 202 417
1
158 LI:1190250,1;2000SEP08720361888 247 938
158 LI:1190250.1:2000SEP08g895347 1 84
158 LI:1190250,1:2000SEP08' 4005442F6 1 328
158 LI:1190250.1:200DSEP08338681 bFb 1 625
158 LI:1190250.1:2000SEP084005442H1 2 287
158 LI:1190250,1;2000SEP087946358H 1 14 762
158 LI:1190250, 7 6250084H 1 67 596
; 2000SEP08
158 LI:1190250,1:2000SEP082809974H 1 67 326
158 LI:1190250,1;2000SEP087607592H1 68 bbb
i 58 LI;1190250.1:2000SEP083159561 H 58 222
1
158 LI:1190250.1:2000SEP08412478H1 375 603
158 LI:1190250. 7 2862291 H1 377 656
;2000SEP08
158 Li:1190250.1;2000SEP08g3934153 378 847
158 LI:1190250,1;2000SEP08g3900180 406 846
i58 LI:1190250.1:2000SEP08391612F1 434 1065
158 LI:1190250,1:2000SEP088040158H 1 442 1055
158 LI:1190250.1:2000SEP08509350H1 107 309
158 LI:1190250.1:2000SEP087946358J2 99 722
158 LI;1190250.1;2000SEP0870821132V1 180 735
158 LI:1190250.1;2000SEP0870822288V1 180 737
158 LI:1190250.1:2000SEP0870818910V1 180 735
158 LI:1190250.1;2000SEP082929935H1 184 470
158 LI:1190250.1:2000SEP082929935F6 184 426
158 LI:1190250.1;2000SEP0870832705V1 719 828
158 LI:1190250.1:2000SEP0841247876 841 1021
158 LI;1190250.1;2000SEP086779840J1 900 1328
158 LI:1190250.1:2000SEP08g2631213 993 1063
158 LI:1190250.1:2000SEP085501816F6 1067 1306
158 LI:1190250.1;2000SEP08550181 bHl 1122 1225
158 LI;1190250.1:2000SEP0855024037H1 471 1049
158 LI:1190250.1:2000SEP08400544276 489 1016
158 LI:1190250.1;2000SEP0870818940V1 508 1082
158 LI;1190250,1:2000SEP0855_024037) 552 1217
_ ._ 1
197
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
158 LI:1190250.1:2000SEP08g5339250 562 1047
158 LI:1190250.1;2000SEP08059074H1 604 769
158 LI:1190250.1;2000SEP0870821167V1 631 1080
158 LI:1190250.1:2000SEP0870822737V1 647 955
158 LI:1190250.1:2000SEP088040158) 1 693 1219
158 LI:1190250.1:2000SEP083672749H 1 81 366
158 L1:1190250.1:2000SEP08653157H 1 82 3 i
2
158 LI:1190250.1;2000SEP08g3000968 312 608
158 LI:1190250.1:2000SEP08338681676 339 821
158 LI;1190250.1:2000SEP0839161281 355 904
158 LI:1190250.1:2000SEP0841247886 375 703
159 LI;1013717.1:2000SEP086798057F8 1 566
159 LI:1013717.1:2000SEP086798057H1 1 529
159 Lt: i Oi 3717.1;2000SEP08679805778 490 1066
i 60 LI:2049125.2:2000SEP0852918 i 8H i 259
i
160 LI;2049125.2:2000SEP0870803952V1 1 661
160 LI:2049125.2:2000SEP085542143H1 81 171
160 LI;2049125.2:2000SEP0870801203V1 73 267
T 60 LI:2049125.2;2000SEP086969152U 1 45 453
161 LI:10923b0.1:2000SEP086752529) 1 258 791
161 LI:10923b0.1:2000SEP08675252988 213 791
1 6l LI;10923b0.1:2000SEP086881093F8 1 605
161 LI:1092360.1:2000SEP08g3597108 494 810
161 LI:10923b0.1:2000SEP08g5364506 349 799
161 LI:10923b0.1:2000SEP08g5590217 452 908
161 LI:1092360.1:2000SEP086881093H1 125 605
1 b2 LI:791524.1:2000SEP082998325H 1 148 431
1 b2 LI:791524.1:2000SEP088050221 H 68 671
1
1 b2 LI:791524.1:2000SEP08299832576 141 606
1 b2 LI:791524. i ; 7752481 H 1 576
~ 2000SEP08 7
1 b2 LI;791524.1:2000SEP082998325F6 148 570
162 LI:791524.1;2000SEP08g5664125 340 652
1 b3 LI:1084555.3:2000SEP08g2004742 235 515
1 b3 LI:1084555.3:2000SEP086457442H 1 1 511
1 b3 LI:1084555.3:2000SEP086452042H 1 1 566
1 b3 LI:1084555.3:2000SEP084053081 H 103 373
1
i 64 LI;815418.2:2000SEP081939605H 1 1643 i 921
1 b4 LI:815418.2:2000SEP086079908H 1 1858 2307
164 LI;815418.2:2000SEP088097467H1 9 637
1b4 LI:815418.2;2000SEP087019587H1 2 481
164 LI:815418,2;2000SEP087696123)1 5 371
164 LI:815418.2:2000SEP0871592951 V1 1443 2126
1 b4 LI:815418.2:2000SEP087720648) 1 594 1173
164 LI;815418.2:2000SEP083524549H1 1247 1373
164 LI;815418.2:2000SEP081404445H1 1256 1526
1 b4 LI:815418.2:2000SEP087700830H 1 377 932
1 b4 LI:815418.2:2000SEP087646833H 1 381 1075
164 LI:815418.2;2000SEP087704184H1 379 1038
198
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID NO: Template ID Component Start Stop
ID
164 LI:815418.2:2000SEP087758306) 1 400 1075
164 LI:815418.2;2000SEP087959989)1 412 1048
164 LI:815418.2;2000SEP08829930H1 1411 1558
1 b4 LI;8 T 5418.2:2000SEP087726033H 1 ' 25 680
164 LI:815418.2:2000SEF087704184) 1 63 656
164 LI:815418.2:2000SEP0871591834V1 1746 2196
164 LI:815418.2:2000SEP085461482H 1 1 243
164 LI:815418.2;2000SEP085513865H1 1 224
164 LI;815418.2:2000SEP085116934H1 1082 1337
164 LI:815418.2:2000SEP087357218H T 1493 1688
164 LI:815418.2:2000SEP0871596065V 1758 2200
1
164 LI:8 T 5418.2:2000SEP087088412H 1 1733 2038
164 LI;815418.2:2000SEP084111224H1 1247 1495
T 64 LI:8154 T 8.2;2000SEP085440737H 1 T 27 T 1391
164 LI:815418.2:2000SEP087757516) 1 1279 1803
164 LI;8154i8.2:2000SEP083805239H1 1279 1543
1b4 LI;815418.2:2000SEP084432282H1 1279 1532
164 LI:815418.2;2000SEP085119476H1 1279 1528
1b4 LI:815418.2:2000SEP083327962H1 1279 1504
1 b4 LI; 8 T 5418.2;2000SEP085950033H 1 1279 1482
164 LI;815418,2:2000SEP081831675H1 1283 1612
164 LI;815418.2;2000SEP084551031H1 1293 1551
164 LI:815418.2:2000SEP086937361 H1 1279 1819
164 LI;815418.2:2000SEP087726033) T T 289 1929
164 LI:815418.2:2000SEP081638772H1 1292 1527
1 b4 LI:8 T 54 T 8.2; T 638676H 1292 1430
2000SEP08 1
164 LI:815418.2:2000SEP083356985H1 1295 1602
164 LI;815418.2:2000SEP08g2028647 1297 1559
164 LI;815418.2;2000SEP0870714789V1 1300 1916
1 b4 L1:815418.2:2000SEP086494084H 1 1307 1956
164 LI:815418.2;2000SEP085585079H1 1320 1561
164 LI;815418.2;2000SEP08g1887342 1316 1694
164 LI;815418.2:2000SEP0840759086 1323 1912
1 b4 LI:8154 T 8.2:2000SEP083539334H 1 1341 1526
164 LI:8 T 5418.2:2000SEP082434087H T T 463 171
T
1b4 LI:815418.2:2000SEP088033808H1 1 594
1 b4 LI:815418.2:2000SEP08585591778 1578 2176
164 LI;815418.2;2000SEP086543688F8 1519 2180
164 LI:815418.2;2000SEP0870713698V1 1026 1'173
1b4 LI;815418.2;2000SEP081646119H1 1438 1676
164 LI:815418.2:2000SEP084401248H 1 802 1028
1 b4 LI:815418.2:2000SEP087679539) 1 1561 1894
164 LI:815418.2;2000SEP087751992H1 1544 2240
164 LI:815418.2;2000SEP082445350H1 1548 1811
164 LI:815418.2:2000SEP08841793H1 1547 1761
164 LI:815418.2;2000SEP086588605H1 1536 1590
164 LI;815418.2:2000SEP081712775H1 1247 1442
l b4 LI:815418.2:2000SEP087368595H 1 473 1095
199
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
i64 LI;815418.2;2000SEP087601736)1 798 1004
l b4 LI:815418.2;2000SEP085852466H 1 1410 1727
164 LI;815418.2:2000SEP081635358H1 1594 1808
1 b4 LI:815418.2;2000SEP086129968H 1 1601 1697
1 b4 LI:815418.2;2000SEP083967245H 1 1605 1770
164 LI:815418.2:2000SEP085274225H 1 1618 1886
164 LI:815418.2:2000SEP081623289H 1 1618 1808
164 LI:815418.2;2000SEP082302331 H1 1618 1766
164 LI:8 i 5418.2:2000SEP08787538H 1 1405 1710
164 LI:815418.2:2000SEP085667061 H1 1406 1635
164 LI:815418.2:2000SEP087054622H 1 1410 1658
764 LI:815418.2;2000SEP081479523H1 1352 1602
164 LI:815418.2:2000SEP088010391 H 1 598
1
164 LI:815418.2:2000SEP082561408H1 1493 ' 1799
1 b4 LI;815418.2:2000SEP087699484H 1 601 1173
164 LI;815418.2:2000SEP08412737078 1897 2187
1 b4 LI:815418.2:2000SEP085162201 F8 13 682
164 LI:815418.2;2000SEP087702057)2 556 1151
i 64 LI:815418.2;2000SEP087369319H 1 1588 2013
164 LI:815418.2:2000SEP0871617263V1 1647 2308
1 b4 Ll;815418.2:2000SEP081255582H 1 1412 1670
164 LI;815418.2:2000SEP08653870578 1896 2184
164 LI:815418.2:2000SEP087632804) 1 905 1530
1b4 LI;815418.2:2000SEP087438620H1 899 1511
164 LI:815418.2;2000SEP08g653173 1783 2062
164 LI:815418.2;2000SEP0871596964V1 1772 2273
164 LI:815418.2:2000SEP085105432H 1 1790 1895
1 b4 LI;815418.2:2000SEP087699484) 1 238 969
164 LI;815418.2:2000SEP087975382H2 312 1037
1b4 LI:815418.2;2000SEP085291313H1 1247 1489
1 b4 LI :815418.2:2000SEP087720601 J 583 1190
1
164 LI:815418.2:2000SEP085401644H 1 1246 1385
164 LI:815418.2:2000SEP083538879H 1 1247 1554
164 LI:815418.2;2000SEP087987963H1 627 1261
1 b4 LI;815418.2:2000SEP084230039N i 1863 2138
l b4 LI;815418.2:2000SEP083899890H 1 1067 1366
164 LI;815418.2;2000SEP083992872H1 1067 1381
164 LI;815418.2:2000SEP087952270H2 476 1122
164 LI;815418.2:2000SEP0871596167V1 1762 2253
164 LI:815418.2;2000SEP085i 62201 T8 1682 2198
164 LI:8154i 8.2:2000SEP088113755H 1 1 548
1 b4 Li:815418.2:2000SEP084438341 T8 1523 2235
164 LI:815418.2:2000SEP087383380H1 1516 2071
164 LI:815418.2;2000SEP087460571 H1 1531 2186
1 b4 LI:815418.2;2000SEP086124892H 1 1001 i 653
164 LI:815418.2:2000SEP088122761 H 196 599
1
~
i b4 LI:815418.2;2000SEP085225160H 1 1409 1681
1 b4 LI:815418.2:2000SEP08" 1461381 1271 1471
_ _-__. H 1
-
~00
7
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
164 LI:815418.2:2000SEP086538705F8 1888 2182
164 LI;815418.2:2000SEP087228045H1 1412 1882
164 LI:815418.2:2000SEP082017950H 1 1633 1949
1 b4 LI:815418.2:2000SEP08800 i 279H 1 535
1
164 LI:815418.2:2000SEP084855067H1 1410 1683
164 LI:815418.2:2000SEP084773987H 1 1047 1329
164 LI:815418.2;2000SEP08612 T 184H 1001 1682
1
164 LI:815418.2:2000SEP083843355H1 1037 1139
164 LI:8154i8.2:2000SEP085018048H1 1042 1299
T 64 LI:B T 5418.2;2000SEP084525432H 1 12 105
1 b4 LI:8 T 5418.2:2000SEP081372242H 1 1489 1747
164 LI:815418.2;2000SEP082100808H1 1087 1351
164 Li.8154 T 8.2:2000SEP087426224H 1 i 082 i 669
164 LI:815418.2;2000SEP087646456)1 791 1296
164 LI:815418.2;2000SEP083175396H1 895 1146
T 64 LI:815418.2:2000SEP082354245H 1 2037 2143
164 LI:815418.2:2000SEP08654368818 2021 2219
164 LI :8154 T 8.2:2000SEP0871 625604V 2037 2319
1
164 LI:815418.2;2000SEP084732632H 1 ~ 1965 2059
1 b4 LI:815418.2;2000SEP086033960H 1 845 1376
1 b4 LI:815418.2:2000SEP088044133) 1 857 1173
164 LI;815418.2:2000SEP088057625)1 844 1523
164 LI;815418.2:2000SEP085070406H1 849 1132
164 LI:815418.2:2000SEP087076001 H 864 1101
1
1 b4 LI:815418.2;2000SEP087406388H 1 866 1485
164 LI;815418.2:2000SEP087738163)1 864 1479
164 LI;815418.2:2000SEP087644181)1 826 1176
1 b4 LI:815418.2;2000SEP087644181 H1 826 1173
164 LI;815418.2;2000SEP087736390) 1 20 703
1 b4 LI:815418.2:2000SEP087702057H 1 552 1151
164 LI:815418.2:2000SEP0.870715511 V 1439 1 b
1 16
164 LI;815418.2:2000SEP088102648H1 812 1485
164 LI:815418.2:2000SEP084399048H2 1270 1525
1b4 LI:815418,2:2000SEP081403839H1 1256 1499
1b4 LI:815418.2;2000SEP08697653H1 1259 1394
164 LI:815418.2:2000SEP08g944425 1268 1668
1 b4 LI:815418.2;2000SEP081259220H 1 1269 1587
1b4 LI;815418.2:2000SEP088001028H1 161 640
164 LI:815418.2:2000SEP087979296H1 1 598
T 64 LI:815418.2:2000SEP08601029H 1 1252 1527
1b4 LI:815418.2;2000SEP087247435H1 1886 2174
1b4 LI:815418.2;2000SEP084457273H1 1494 1801
164 LI:8 T 5418.2:2000SEP08200841 OH 1495 1593
1
164 LI:815418.2;2000SEP087456389H1 1863 2040
i b4 LI:815418.2:2000SEP087456775H 1 1867 2034
1 b4 LI:815418.2:2000SEP088015933) 1 1552 2034
1 b4 LI:815418.2:2000SEP08622713H 1 1555 1802
164 LI:815418.2:2000SEP088043833) 1 1580 2043
_ -201
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
164 LI:815418.2:2000SEP086314259H1 1557 2047
164 LI;815418.2:2000SEP086115729H1 1562 1846
1 b4 LI:815418.2:2000SEP0824531 O1 H 1560 1704
1
164 LI:815418.2;2000SEP082238844H1 1565 1827
164 ~ LI:815418.2:2000SEP082238836H1 1565 1827
164 ~ LI:815418.2:2000SEP086040576H1 1570 2034
1 b4 LI:815418.2;2000SEP085101212H 1 1570 1746
1 b4 LI:815418.2:2000SEP086831007) 1 1573 1795
164 LI:815418.2;2000SEP08g651765 1578 1944
164 LI:815418.2:2000SEP088047502H1 1581 2171
164 LI;815418.2:2000SEP083020957H1 1358 1603
1 b4 LI:815418.2:2000SEP081927501 H 1390 1690
1
164 LI:815418.2;2000SEP082014262H1 1395 1672
164 LI:815418.2:2000SEP087661174)1 1397 1856
164 LI:815418.2:2000SEP083561117H1 1397 1737
1b4 LI:815418.2:2000SEP085991862H1 1397 1701
164 LI;815418.2:2000SEP083373054H1 1400 1693
164 LI:8 i 54 i 8.2:2000SEP083333394H 1 1403 i 7
i 3
1b4 LI;815418.2:2000SEP084106163H1 1405 1703
164 LI:815418.2:2000SEP081906326H1 1364 1556
164 LI;815418.2;2000SEP0871594488V1 1376 1783
164 LI:815418.2;2000SEP081444273H1 1374 1656
164 LI:815418.2;2000SEP083026558H1 1380 1719
1 b4 LI:815418.2:2000SEP08633226H 1 1386 1656
164 LI;815418.2;2000SEP083983144F6 1109 1487
1 b4 LI:815418.2:2000SEP085578114H 1 1426 1682
164 LI:815418.2:2000SEP0871592121 V 1581 2203
1
164 LI;815418.2:2000SEP087719344)1 6 657
164 LI:815418.2:2000SEP085759255H1 1 295
164 LI:815418.2:2000SEP0871597062V1 1510 2196
1 b4 LI:815418.2:2000SEP081792905H 1 1505 1804
164 LI:815418.2:2000SEP083761879H1 921 1148
1 b4 LI:815418.2:2000SEP082194839H 1 926 1173
1 b4 LI;815418.2:2000SEP08g2009100 ~ 932 1173
164 LI:815418.2:2000SEP081813648H1 951 1169
164 LI;815418.2:2000SEP082464660H1 951 1173
164 LI;815418.2:2000SEP08g1695375 976 1155
1 b4 LI:815418.2:2000SEP08664490H 1 969 1173
1 b4 LI:815418.2:2000SEP084438341 H 982 1173
1
1 b4 LI:8 i 5418.2;2000SEP084098865H 1 976 1176
164 LI:815418.2;2000SEP086610789)1 973 1614
164 LI:815418.2:2000SEP084849354H1 997 1173
1 b4 LI:815418.2;2000SEP086610789H 1 984 1 b
14
1 b4 LI:815418.2:2000SEP08g 1963049 1002 1179
164 LI:815418.2:2000SEP085275129H1 1412 1683
1b4 LI;815418.2:2000SEP086196729F8 1772 1895
1 b4 LI:815418.2:2000SEP087700830) 1 1 639
1 b4 LI:815418.2:200OSEP088048312H 1 1752 2185
202
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
164 LI;815418.2;2000SEP087438277H1 743 1173
1 b4 LI:815418,2:2000SEP088037826) 1 763 1399
164 LI:815418.2;2000SEP087640014)2 767 1352
164 LI:815418.2:2000SEP088044155) 1 777 1173
1 b4 LI;8 i 54i 8.2;2000SEP084999883H 1 891 1 i
82
1b4 LI;815418,2;2000SEP084770709H1 1247 1509
1 b4 LI;815418.2:2000SEP083795463H 1 1247 1522
164 LI;815418.2;2000SEP087720648H1 158 774
164 LI:815418,2:2000SEP087605501 J 88 734
1
164 LI;815418.2;2000SEP08775830bHi 147 822
164 LI;815418,2;2000SEP087638818H1 158 637
164 LI;815418,2;2000SEP087959989H1 158 831
1 b4 L1:87 5418.2;2000SEP087646833) 1 158 783
164 LI;815418.2;2000SEP084161355H1 1220 1523
1 b4 LI:815418.2:2000SEP087736390H 1 1230 1966
164 LI;815418,2;2000SEP0855017124)1 499 654
164 LI:815418,2:2000SEP085855917F8 1568 2166
1 b4 LI:815418.2;2000SEP08384335578 1515 2220
164 LI;815418.2;2000SEP087618260H1 1517 2046
164 LI:815418,2;2000SEP085105938H1 1922 2215
164 LI:815418.2;2000SEP08g7037738 1930 2317
164 LI:815418,2;2000SEP08g7156198 1965 2317
164 LI;815418.2;2000SEP085855917H1 2016 2120
1 b4 LI:815418,2:2000SEP087984084H 1 480 976
164 LI:815418.2;2000SEP088033808)1 1588 2324
1 b4 LI:815418,2:2000SEP088008591 H 1 694
1
164 LI:815418,2;2000SEP086440272H1 1006 1317
1 b4 LI:815418.2:2000SEP087089726H 1 1902 2044
164 LI;815418,2;2000SEP087604808H1 1907 2167
1b4 LI;815418.2:2000SEP08- 7581949H1 1907 2133
164 LI:815418,2:2000SEP082570255H1 1015 1173
164 LI;815418,2;2000SEP083843355F8 1037 1774
164 LI:815418,2;2000SEP087966935H1 1906 2188
1b4 LI:815418.2;2000SEP084296937H1 907 1142
1b4 LI;815418,2:2000SEP086937329H1 626 1184
1 b4 LI:815418.2;2000SEP08g6577230 1916 2321
164 LI:815418.2:2000SEP08692642H 1 1417 1 b
12
164 LI;815418.2;2000SEP087620414)1 1423 2132
1 b4 LI:815418,2:2000SEP081267495H 1 1118 1359
164 LI:815418.2;2000SEP084672356H1 1213 1493
1b4 LI:815418,2:2000SEP08398314476 1139 1515
164 LI;815418,2:2000SEP0871654110Vi 1750 2273
1 b4 LI;815418.2;2000SEP088001501 H 1 406
1
164 LI;815418.2:2000SEP087977294H1 1 714
164 LI;815418,2;2000SEP082963978H1 1449 1700
164 LI:815418.2;2000SEP085838520H1 1450 1720
164 LI:815418.2;2000SEP081419517H1 1450 1714
164 LI:BI 5418.2:2000SEP087159_6483V 1735 2196
1
_
203
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
164 LI:815418.2:2000SEP081622188H1 1462 1686
1 b4 LI:815418.2;2000SEP084549877H 1 1469 1610
164 LI:815418.2:2000SEP08805118H 1 1473 1671
164 LI:815418.2;2000SEP081905966H1 1475 1763
l b4 LI:815418,2:2000SEP0870 T 130H 1487 1779
1
1 b4 LI;815418,2:2000SEP084854967H 1 1410 1683
164 LI:815418,2:2000SEP082240255H2 1411 1663
164 LI:815418,2:2000SEP082189166H1 1411 1524
1b4 LI;815418.2;2000SEP0871596336V1 1678 2106
164 LI:8 i 5418,2:2000SEP086476992H i 1692 2302
164 LI;815418,2;2000SEP087437046H1 1755 2318
164 LI;815418,2:2000SEP0871595986V1 1722 2294
1 b4 LI :815418.2:2000SE7676864) 1 1724 1809
P08
164 LI:815418.2:2000SEP087452964H1 647 1173
164 LI:815418,2;2000SEP087453093H1 647 1173
164 LI;815418,2:2000SEP087453048H1 647 1182
164 LI;815418.2:2000SEP087453010H1 647 1173
1b4 LI:815418.2;2000SEP087438496H1 655 1097
1 b4 LI:BI 5418.2:2000SEP087426104H l 675 1187
1 b4 LI:815418,2:2000SEP087455425N 1 727 ~ 1173
1 b4 LI:815418,2;2000SEP087601774) 1 729 1173
1 b4 LI:8154 T 8.2:2000SEP08g2070032 1272 1672
164 LI;815418,2:2000SEP084969651H1 1271 1532
164 LI:815418,2:2000SEP08g2189893 1271 1694
T64 LI;8T5418.2;2000SEP082170391HT 1271 1460
164 LI:815418.2:2000SEP087660291 H1 1282 1894
1 b4 LI:815418.2:2000SEP0871596226V 1989 2179
1
1 b4 LI;815418,2:2000SEP0871596061 V1 .1991 2179
1 b4 LI:815418,2;2000SEP083234752F6 1978 2356
164 LI:815418.2:2000SEP083234752H1 1957 2022
164 LI:815418,2;2000SEP0871592276V1 1674 2194
1b4 LI;815418.2:2000SEP087646456H1 1469 1846
1 b5 LI:4167bb.1:2000SEP084031856H 1 1252 1486
1b5 LI;4167bb.1:2000SEP086199132H1 1561 2074
165 LI:416766.1:2000SEP0870856083V1 1953 2465
165 LI;416766.1;2000SEP08g4190333 681 1064
1 b5 LI:416766.1:2000SEP08g4334177 2909 3276
165 LI:4 i 6766. i 708551 ObV 2939 35 i
:2000SEP08 1 1
165 LI;416766.1:2000SEP08g2715986 2943 3267
1 b5 LI:41 b7bb,1:2000SEP086513330H 1 3000 3292
1 b5 LI:416766, T :2000SEP08g 1242884 680 1022
1 b5 LI:416766.1:2000SEP087211424H 1 2357 2667
1 b5 LI:416766.1:2000SEP085694841 T6 728 1171
165 LI:416766.1:2000SEP08g2240560 741 1234
1 b5 LI;416766,1:2000SEP08g6438877 672 1094
165 LI:416766,1:2000SEP08g2070577 2906 3275
1 b5 LI:41 b766.1;2000SEP08g3665440 675 1140
l 65 LI:4l 6766.1:2000SEP08g5231632 675 17 Ob
~U4
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
165 LI;416766.1;2000SEP08g6040151 678 1130
1 b5 LI;416766.1:2000SEP08243195H 1 3007 3127
165 LI;41676b.1;2000SEP08296137376 3005 3227
165 LI:41 b7bb.1:2000SEP087011821 F8 673 T 20
i
165 LI:41 b7bb.1;2000SEP08g3679253 675 1146
165 LI;416766.1;2000SEP083515501 H 2101 2344
1
165 LI:41 b7b6.1:2000SEP0871225989V 2357 2930
1
165 LI:416766.1:2000SEP085695241 T8 884 1339
165 LI:41 b7bb.1;2000SEP08g 1367787 1001 1619
165 LI:41 b7bb,1;2000SEP08g5554573 675 1154
165 LI:41 b7bb.1;2000SEP087011821 H1 675 1121
165 LI;41676b. T :2000SEP0870854872V 2807 3398
T
i 65 LI;41 b7bb.1:2000SEP08g4629623 28 i 9 3267
165 LI:4167b6.1:2000SEP08g5512397 2827 3268
165 LI:41 b7bb.1:2000SEP08g5661867 2843 3298
1b5 LI;4167bb.1:2000SEP08g1367813 806 1584
1 b5 LI:416766.1:2000SEP08g5511864 2858 3268
165 LI;41 b7bb.1;2000SEP085445604H 1 2856 3097
165 LI;4167b6.1:2000SEP081251913H1 3132 3273
165 LI:41 b7bb.1;2000SEP081251913F6 3138 3216
165 LI:416766.1:2000SEP082853591 Tb 3149 3559
1 b5 LI:416766.1:2000SEP08g897183 3359 3638
165 LI;416766.1:2000SEP08g2057056 2513 2870
165 LI;4167b6.1;2000SEP082551309H1 2437 2682
165 LI:4167bb.1:2000SEP0870856882V1 2695 3332
165 LI:416766.1:2000SEP084787383H1 2794 3046
165 LI:416766.1:2000SEP082961373F6 3012 3262
165 LI;4167b6.1:2000SEP082961373H1 3014 3142
165 LI:416766.1:2000SEP085308958H1 3051 3271
1 b5 LI:4167bb.1;2000SEP087169394H 1 1 343
1 b5 LI:416766.1:2000SEP08377885979 212 607
1 b5 LI:4167b6.1;2000SEP08377885978 218 573
1 b5 LI:41 b7bb.1:2000SEP083778859F8 221 733
1 b5 LI:4l 67bb.1:2000SEP083778859F9 223 736
165 LI;41b7b6.1:2000SEP083778859H1 218 554
1 b5 LI:4167bb.1;2000SEP087664249H 1 493 1063
l b5 LI;416766.1;2000SEP086011335681 613 1062
1 bb LI;1171008.2:2000SEP088068537) 1 12 456
1 bb LI;1171008.2:2000SEP08g 1984991 1 260
166 LI:1171008.2:2000SEP08g4187090 1 210
1 b7 LI;1169888.3:2000SEP08g4082551 1 249
1 b7 LI:1169888.3:2000SEP08g6655195 5 260
167 LI:1169888.3;2000SEP083069995F6 54 502
1 b7 LI:11 b9888.3:2000SEP083069995H 1 54 348
1 b7 LI:1169888.3:2000SEP087598547H 1 154 710
1 b7 LI:11 b9888.3:2000SEP085678086H1 170 353
167 LI:1169888.3:2000SEP084731429H1 209 476
167 LI:1169888.3:2000SEP084387033H 1 264 519
_._ - _
-_.
20~
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
1 b7 LI:1169888.3:2000SEP088107619H 1 382 636
1 b7 LI:11 b9888.3;2000SEP082170650H 1 409 655
1 b7 LI:1169888.3:2000SEP085069257H 1 544 834
1 b7 LI: T T b9888.3:2000SEP086883261 F8 550 903
1 b7 LI:1 i b9888.3;2000SEP086883261 H 550 969
1
1 b7 LI:1169888.3;2000SEP086052251 J 660 1024
1
167 LI:1169888.3:2000SEP087939216H 1 836 1314
1 b7 LI:1169888.3:2000SEP088107287H 1 853 992
1 b7 LI:11 b9888.3:2000SEP088107287) 1 853 992
167 ' LI:1169888.3:2000SEP08g6656112 1 266
167 LI:11 b9888.3:2000SEP08g4086074 1 249
168 LI;4T 2592.1:2000SEP083722057H 1 805 1116
168 LI:4 T 2592. T 7695603) 1 587 1099
:2000SEP08
1 b8 Ll:4T 2592, i 6704220H 1 698 931
:2000SEP08
1 b8 L1:412592.1:2000SEP085321546F9 768 1275
168 LI;412592.1:2000SEP085302969F8 769 1327
1 b8 LI:412592.1:2000SEP087707755H 1 221 845
1b8 , LI;412592.1:2000SEP087623656H1 216 843
1 b8 LI;412592.1;2000SEP087611095) 1 227 909
~
1 b8 LI:412592.1;2000SEP08069300H 1 191 307
i 68 LI:412592.1:2000SEP088035862) 1 197 721
1 b8 LI;412592.1:2000SEP087728679) 1 217 842
T 68 LI:412592.1:2000SEP087441308H 1 211 811
T b8 LI:412592. T :2000SEP086907021 H 1 360
1
1 b8 LI:412592.1:2000SEP082573121 H 1 1 6l
1
1 b8 LI:412592.1;2000SEP08797431 6H 313 912
1
1 b8 LI;412592.1:2000SEP087623823H 1 318 809
1 b8 LI:4 T 2592.1;2000SEP087754904H 1 442 763
1 b8 LI;412592.1:2000SEP087696804) 1 543 1107
168 LI:412592.1:2000SEP087707 T 80J 257 927
1
1 b8 LI:412592.1:2000SEP088084683H 1 271 938
168 LI;412592.1:2000SEP088083727H1 271 958
1 b8 LI:412592.1:2000SEP088085224H 1 271 806
1 b8 LI:412592.1:2000SEP086599039H 1 286 399
1 b8 LI:412592.1:2000SEP087723213H2 309 890
168 LI;412592.1:2000SEP087620469H1 229 816
T 68 LI:412592. T :2000SEP08493 T 878T8 896 1 T
33
1 b8 L1:412592. i :2000SEP087439217H 1 9 T 9 1501
1 b8 LI:412592.1:2000SEP087743523) 1 1031 1174
169 LI:349808.1:2000SEP086819387H1 1704 2094
169 LI:349808.1:2000SEP086819295H1 1705 2094
169 LI;349808.1:2000SEP083597372H1 1 287
169 LI:349808.1:2000SEP081b27bbOH1 1090 1292
169 LI:349808.1:2000SEP087948057H1 1185 1758
169 LI:349808. T :2000SEP087110908F8 2576 2855
169 L1:349808.1:2000SEP087429309H1 2046 26T
1
1 b9 LI:349808.1:2000SEP08675535788 2403 2855
1 b9 LI:349808.1;2000SEP083_579765H 22 323
_.__ 1
_
.206 ' --_
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
1 69 Li:349808. T :2000SEP087281181 H 34 136
1
169 LI:349808.1;2000SEP086604801 H1 1061 1229
1 b9 LI:349808. 1 :2000SEP088035241 J 950 1 632
1
169 LI;349808, 1 :2000SEP082495529H 1 557 628
169 LI:349808.1:2000SEP087689446) 1 582 1147
169 LI:349808.1;2000SEP087584138H1 601 1169
l b9 LI:349808.1;2000SEP082306387H 1 613 878
169 LI:349808.1;2000SEP088018981 J 793 1402
1
1 b9 LI;349808, 1 ;2000SEP088035241 H 1415 1994
1
169 LI:349808.1;2000SEP086914347H 1 1242 1643
169 LI;349808.1;2000SEP081415229H1 1544 1811
169 LI:349808.1;2000SEP08803461 OJ 1896 2588
T
169 LI:349808.1:2000SEP086819387) 1 1705 2094
169 LI:349808.1;2000SEP086819295) 1 1705 2092
169 LI:349808.1:2000SEP08681938788 1705 2086
1 b9 LI:349808.1:2000SEP082653094H 1 1722 1947
169 LI;349808.1:2000SEP086819387F8 1791 2094
169 LI;349808.1:2000SEP086910219)1 1874 2060
169 LI:349808.1;2000SEP083381265H 1 2434 2701
169 LI;349808.1;2000SEP086755357)1 2433 2854
169 LI;349808.1:2000SEP087110908H1 2575 2854
169 LI;349808.1;2000SEP083319124F6 40 566
169 LI;349808.1;2000SEP083319124H1 40 314
169 LI;349808.1;2000SEP08g4080691 56 303
169 LI;349808.1:2000SEP087994037H1 68 646
169 LI;349808.1:2000SEP087253443H1 69 521
169 LI:349808.1;2000SEP08546961 H 1 9 288
1 b9 LI:349808.1;2000SEP081430402F6 477 779
1 b9 LI:349808.1;2000SEP081430402H 1 477 723
169 LI:349808.1;2000SEP087689446H 1 334 595
1 b9 LI:349808.1:2000SEP088034434) 1 348 1007
169 LI:349808.1:2000SEP087405584H1 306 794
169 LI:349808.1;2000SEP088117848H1 77 692
170 LI:3491b4.2:2000SEP087622234H1 1 446
170 LI;349164.2;2000SEP087622884H1 1b2 769
170 LI;349164.2:2000SEP086911488H1 588 1066
170 LI:3491 b4.2:2000SEP086274443H2 352 945
170 LI;349164.2:2000SEP083027742H1 431 716
170 LI;349164.2:2000SEP084301890H1 623 857
170 LI;3491b4.2:2000SEP086906443H1 645 1147
170 LI:3491b4.2;2000SEP0870857325V1 838 1059
170 LI;349164:2:2000SEP084457564H1 938 1178
170 LI:349i64.2:2000SEP084454771H1 944 1197
171 LI:205413.1;2000SEP08g2115617 1734 2232
171 LI:205413.1:2000SEP087950063H1 1730 2205
171 LI:205413.1:2000SEP08g 1548634 1462 1664
171 LI:205413.1:2000SEP08g 1476869 1462 1603
171 LI:20541 3.1:2000SEP082659489H 1 1508 1592
_-207
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
171 LI:205413.1;2000SEP082659489F6 1508 2121
171 LI:205413.1:2000SEP087707719) 1 1544 2120
171 LI:205413.1:2000SEP08g2115614 1729 2232
171 LI:2054 i 3. i 5314004H 1 103 345
:2000SEP08
171 LI;205413.1;2000SEP08g3109828 1738 2124
171 LI:205413.1;2000SEP082505546N 1 i 794 1917
171 LI:205413.1;2000SEP083247411 F6 833 1105
171 LI:205413.1:2000SEP083247411H1 833 931
171 LI;205413. i :2000SEP086541837H 1 462 957
171 LI;205413.1:2000SEP087203862H1 467 751
171 LI:205413.1:2000SEP084541365H1 1165 ~ 1407
171 LI:205413.1:2000SEP083253915Th 974 1429
171 LI:205413.1:2000SEP084291253F6 143 474
171 LI:205413.1:2000SEP084291253H1 145 396
171 LI:205413.1:2000SEP087411467H1 247 792
171 LI;205413.1:2000SEP08195208386 2042 2214
171 LI;205413.1;2000SEP086629950U1 2042 2214
171 LI:205413.1;2000SEP087386810H1 273 874
171 LI:205413.1:2000SEP083024202H1 313 598
171 LI:205413.1:2000SEP087411330H1 576 1117
~
171 LI:205413.1:2000SEP07950063) 1 1839 2418
8
171 LI;205413.1;2000SEP087065386H1 1899 2344
171 LI;205413.1:2000SEP085172246H1 1923 2150
171 LI:205413.1:2000SEP086873144H1 1956 2344
171 LI;205413.1;2000SEP082070268H1 553 702
171 LI:205413.1:2000SEP08g 1126720 493 725
171 LI:205413.1:2000SEP08g318198 560 859
171 LI:205413.1:2000SEP08178046H1 1028 1109
171 LI:205413.1:2000SEP087658127H1 992 1522
171 LI;205413.1:2000SEP08g3593212 1028 1336
171 LI:205413.1:2000SEP087707719H1 869 1514
171 LI:205413.1:2000SEP08325391586 956 1417
171 LI:205413.1:2000SEP087274523H1 1220 1625
171 LI:205413.1:2000SEP085996466H1 598 1217
171 LI;205413.1:2000SEP086101305H1 1215 1442
171 LI;205413.1:2000SEP082415b58Hi 2425 2616
171 LI:205413.1:2000SEP084757957H1 2464 2640
171 LI:205413.1:2000SEP084757957F6 2537 2848
171 LI:205413.1:2000SEP08g786768 2564 2857
171 LI;205413.1:2000SEP084757957Th 2704 2864
171 LI:205413.1;2000SEP081952083T6 2706 2762
171 LI:205413.1:2000SEP087073566H1 2718 2848
171 LI;205413.1:2000SEP081733231 Fb 2754 2848
171 LI;205413.1:2000SEP081733231H1 2754 2848
171 LI:205413.1:2000SEP08g812880 1 279
171 LI:205413.1:2000SEP081952083H1 2042 2214
171 LI:205413.1:2000SEP08g824629 2 285
171 LI:205413.1:2000SEP085311859H 1 103 365
_._
208
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
17i LI:205413.1:2000SEP08g769342 2 234
171 LI:205413.1;2000SEP083253915N 1 956 1228
171 LI:205413.1;2000SEP087360936H 1 1074 1593
171 LI:205413.1:2000SEP087314994H i 1161 1592
171 LI:205413,1:2000SEP08g6299856 1223 1653
171 LI:205413.1;2000SEP08g4457741 1283 1466
171 LI;205413,1:2000SEP08g824868 1385 1759
171 LI:205413.1;2000SEP08g764871 1395 1759
17 i LI;205413. i :2000SEP081412476H 1 1430 1592
171 LI;205413.1:2000SEP08289182876 1174 1430
171 LI;205413.1:2000SEP083331088H1 691 947
171 LI:205413. i ;2000SEP083524383H1 2102 2411
171 LI;205413.1:2000SEP08g1275525 2170 2492
171 LI:205413.1;2000SEP086629920U1 2246 2642
171 LI;205413.1:2000SEP088068036J1 2271 2616
i 71 LI:205413.1;2000SEP087950631 J 2079 2394
1
171 LI:205413,1:2000SEP087950631 H 2100 2394
1
171 LI;205413,1:2000SEP086982879H1 1 315
i72 LI:205i508.2:2000SEP085770980H1 1073 1594
172 LI:2051508.2:2000SEP085770980F8 1085 1641
172 LI:2051508,2:2000SEP0877b548bHi 1353 1941
172 LI:2051508,2;2000SEP08gi303480 1368 1608
172 LI:2051508.2;2000SEP086355546H1 1473 1677
172 LI;2051508,2;2000SEP086568067H1 0 1 62
172 LI;2051508.2:2000SEP086267543F8 1 657
i 72 LI:2051508,2;2000SEP086568067F8 1 665
172 LI:2051508,2:2000SEP086267543H1 1 536
i 72 LI:2051508.2:2000SEP08626754378 158 699
172 LI:2051508,2;2000SEP087765486J1 501 1 i50
172 LI:205i 508.2:2000SEP085625452H 1 511 641
172 LI:2051508.2;2000SEP087388455H1 649 1075
7 72 LI:205 i 508.2;2000SEP087330071 H 870 i 307
1
173 LI:346242.2:2000SEP083679845Hi 1018 1218
173 LI:346242.2:2000SEP08408199378 983 1487
173 LI:346242.2;2000SEP087620566Hi 1003 1637
173 LI:34b242,2;2000SEP087649709H1 1003 1572
173 LI:346242.2:2000SEP087327034H 1 955 1471
173 LI:346242.2:2000SEP088056268) 1 730 1284
173 LI:346242.2:2000SEP0877 i 8357H 841 1325
i
173 LI;346242.2;2000SEP087468459H1 946 1471
173 LI;346242,2:2000SEP087662015)1 1026 1642
i 73 LI:346242.2:2000SEP086906628H 1 1031 14 i
9
173 LI;346242,2:2000SEP08g1685690 1060 1433
173 LI:34b242.2:2000SEP084180807H1 1118 1412
173 LI;346242.2:2000SEP084180807F6 1 T T i 487
8
173 t1:34b242.2;2000SEP082720980H1 1265 1373
173 LI;34b242.2;2000SEP087978939H1 1 479
i 73 LI:346242,2:2000SEP087741 i 09H 17 487
i
209
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
173 LI:346242.2:2000SEP088017662) 1 24 630
173 LI:346242.2:2000SEP086780866H 1 178 473
173 LI:346242.2;2000SEP087724708) 1 336 473
173 LI:34b242.2:2000SEP086764336) 1 339 472
173 LI:34b242.2:2000SEP08800b8b4Hi 451 1094
i 73 LI:34b242.2:2000SEP086915577H 1 615 697
173 LI;346242.2;2000SEP086797389H1 691 1241
173 LI:346242.2:2000SEP084052477H 1 702 784
i 73 LI:34b242.2;2000SEP083679845F6 1018 1097
173 LI:34b242.2;2000SEP082720988F6 1265 1373
773 LI;346242.2;2000SEP0871220924Vi 1275 1503
173 LI;346242.2;2000SEP08272098876 1301 1372
174 LI;2052717.1;2000SEP085067091 T9 828 1475
174 LI:2052717.1:2000SEP084894635F6 1126 1550
174 LI;20527i7.1:2000SEP084894635H1 1126 1402
174 LI:2052717. i 258300276 1173 1451
:2000SEP08
174 LI:2052717.1:2000SEP0885540945 1206 1551
174 LI:2052717.1:2000SEP085696575H1 1275 1532
174 LI:2052717.1:2000SEP088273554 624 810
174 LI:2052717.1:2000SEP086595153) 1 1490 1788
174 LI:2052717.1:2000SEP083002561 T7 1603 1729
174 LI:2052717.1:2000SEP083002561 H 1603 1763
1
174 LI;2052717.1;2000SEP086148958H 1 222 789
174 LI:2052717, i 7714444H 1 233 841
:2000SEP08
i 74 LI:2052717.1;2000SEP085067091 H 520 763
1
i 74 LI:2052717.1;2000SEP085067091 F9 523 1007
174 LI:2052717.1:2000SEP085063731 F8 543 1081
174 LI;2052717.1:2000SEP083002561 Fb 1603 1763
174 LI:2052717.1;2000SEP08g T 147397 i b 16 17 i
3
i 74 LI:2052717.1;2000SEP0883593657 1697 1808
'
174 LI;2052717.1;2000SEP0887041600 1703 1808
i 74 LI:2052717.1:2000SEP082583002F6 552 1057
174 LI;2052717.1:2000SEP082583002H1 552 819
174 LI:2052717.1;2000SEP086619463H1 1 57i
175 LI:406b68.2;2000SEP085672240H1 1258 1537
175 LI:40b668.2:2000SEP087002774H 1 i 601 2268
175 LI;406668.2;2000SEP087626312) 1 1261 1948
175 LI:406668.2:2000SEP08g 1718534 1278 1350
175 LI:40b668.2:2000SEP08i 390315H i 186 1449
i
175 LI:4066b8.2:2000SEP083744324F6 1018 1356
175 LI:40b668.2:2000SEP0881779107 1337 1662
175 LI;406668.2:2000SEP085336594H1 1334 1569
175 LI;406668.2:2000SEP084933068H 1 1336 1623
175 LI:406668.2:2000SEP0886703684 1600 2038
175 LI:406668.2:2000SEP087024005H 1 1359 1809
T 75 LI;406668.2:2000SEP081390315F6 1185 1604
175 LI;406668.2:2000SEP0885397998 2262 2362
175 LI:40b668.2;2000SEP08410500H1 2284 2393
_
210
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Starf Stop
NO: ID
175 LI:40b668.2;2000SEP085434671H1 2167 2384
175 LI:40b668.2:2000SEP088042801 H 1347 1638
1
175 LI:406668.2:2000SEP083336387H1 69 296
175 LI:40bb68.2:2000SEP086788039H1 195 340
175 LI:406668.2;2000SEP083336387F6 69 413
175 LI;40b668.2;2000SEP087674468)1 306 806
175 LI:40b668.2:2000SEP088042709) 1 736 1233
175 LI :40b668.2:2000SEP088042801 J 749 1313
1
175 LI:406668.2:2000SEP084126078H1 906 1084
175 LI:40b668.2:2000SEP084212553H1 1295 1516
175 LI:40b668.2:2000SEP08g 1718535 2092 2395
175 LI:406668.2;2000SEP083723230H1 2092 2392
175 LI:40b668.2:2000SEP08g 1780142 2101 2392
175 LI:40bb68.2:2000SEP082441933H1 2118 2310
175 LI:40bb68.2:2000SEP083744324H 1 1018 1251
175 LI:406668.2;2000SEP086454883H1 11 481
175 LI:406b68.2:2000SEP086569407H 1 34 466
175 LI;406668.2;2000SEP087723868) 1 1 461
175 LI;406668.2;2000SEP087985745H2 1 606
175 LI;406668.2:2000SEP083473241H1 8 259
175 LI:4066b8.2:2000SEP0882347904 1895 2391
175 LI:406668.2:2000SEP08- 82269419 1907 2391
175 LI:406668.2;2000SEP08~ 83149881 1297 1602
175 LI:406668.2;2000SEP0885660211 1936 2356
175 LI:406b68.2;2000SEP0885053468 1908 2391
175 LI;406668.2:2000SEP0884988156 1912 ' 2385
175 LI;406668.2:2000SEP0885440700 1928 2392
T 75 LI:40b668.2:2000SEP08493306876 2001 2340
175 LI:40b668.2;2000SEP085022603F9 1636 2065
175 LI;406b68.2;2000SEP086576919H1 1705 2168
175 LI:40b668.2;2000SEP08374432476 1725 2318
175 LI:406668.2:2000SEP087626312H1 1734 2340
175 LI:406668.2:2000SEP08461495876 1766 1952
175 LI;406668.2:2000SEP086870730H1 1832 2347
175 LI;4066b8.2:2000SEP08502260378 1845 2219
175 LI:40bb68.2:2000SEP087369624H 1 1894 2387
175 LI:4066b8.2;2000SEP085022603H1 1603 1732
175 LI:40b668.2;2000SEP084614958H1 1003 1244
175 LI:4066b8.2;2000SEP08- 4614958F6 1003 1573
175 LI:406668.2:2000SEP08875654H1 921 1167
175 LI:406668.2:2000SEP088042709H1 977 1649
176 LI:1178352.1;2000SEP0870867102V1 1509 1808
176 LI:1178352.1:2000SEP0871229547V1 241 896
176 LI:1178352.1;2000SEP0870868356V1 1129 1765
176 LI:1178352.1;2000SEP0871229578V 236 908
1
176 LI:1178352.1;2000SEP0870867476V1 1267 1808
176 LI:1178352.1;2000SEP0871300356V 21 O1 2550
1
176 LI:I 178352.1:2000SEP08_ 71228758V1 1070 1548
211
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO; ID
176 LI:1178352.1;2000SEP0870868887V1 609 1119
176 LI:1178352.1:2000SEP0870869369V1 376 991
176 LI;1178352.1:2000SEP0871300122V1 2079 2563
176 LI:1178352.1;2000SEP08g 1924187 2232 2510
176 LI:1178352.1;2000SEP08g2817010 2129 2509
176 LI:1178352.1;2000SEP08g2903B90 2062 2509
176 LI:1178352.1;2000SEP08g2907503 2333 2509
176 LI;1178352.1;2000SEP08g2818919 2070 2509
176 LI:1178352.1:2000SEP08g2563340 2211 2509
176 LI:1178352.1;2000SEP08g2904085 2066 2509
176 LI: l l 78352.1:2000SEP087947363) 7 l 573 2097
176 LI:1178352.1;2000SEP087947363H 1 1574 2086
176 LI:1178352.1;2000SEP087286502H1 1512 2011
176 LI:1178352, i 70995572V 1345 1959
:2000SEP08 1
176 LI:1178352.1:2000SEP086946552H 1 1585 i 91
i
176 LI;1178352.1:2000SEP086752529F8 1249 1851
176 LI;1178352.1:2000SEP088107756) 1 1473 1671
176 LI;1178352.1;2000SEP087667535H 1 825 1470
176 LI:1 i 78352.1;2000SEP0870867282V 818 1451
1
176 LI:1178352.1:2000SEP087437082H1 1015 1421
176 LI;1178352.1;2000SEP0871230474V1 787 1369
176 LI:1178352.1;2000SEP0870870110V1 1052 1355
~
176 LI;1178352.1:2000SEP0870818444V 982 1348
1
176 LI;1178352.1:2000SEP0871230196V1 737 1324
176 LI:1178352.1:2000SEP086918556H 1 819 1191
176 LI;1178352.1;2000SEP0870839330V1 592 1013
176 LI:1178352.1;2000SEP087280482H 1 517 987
176 LI:1178352.1:2000SEP0871230041 V1 238' 757
176 LI:1178352.1:2000SEP0870869713V1 121 743
176 LI:1178352.1;2000SEP087372475H2 147 700
176 LI:1178352.1;2000SEP0870866849V1 122 660
176 LI;1178352.1;2000SEP0870870780V 122 630
1
176 LI:1178352.1:2000SEP087624286) 1 1 630
176 LI:1178352.1:2000SEP0871299463V 2453 2550
1
176 LI:1178352.1:2000SEP08g5554719 2642 2794
176 LI:1178352.1;2000SEP087318683H 1 2638 2794
176 LI:1178352.1:2000SEP08996955H 1 2560 2794
176 LI:1178352.1;2000SEP08840063H1 2619 2794
176 LI:1178352.1;2000SEP086336473H1 2269 2761
176 LI:1178352.1:2000SEP08647339H 1 2473 2731
176 LI:1178352.1;2000SEP0870997545V1 1976 2587
176 LI;1178352.1;2000SEP08g7155567 2331 2575
176 LI:1178352.1;2000SEP0871300294V1 2067 2566
176 LI:1178352.1:2000SEP0870997554V1 2045 2566
176 LI:1178352.1:2000SEP088018282) 1 1977 2565
176 LI:1178352.1;2000SEP0870870249V1 201 830
176 LI;1178352.1:2000SEP0870867112V1 632 1046
176 LI:1178352.1;2000SEP0870997702V1 2103 2550
__- _
_
212 _
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
176 LI:1178352,1:2000SEP0870996019V 2139 2550
1
176 LI:1178352.1:2000SEP0871360795V 2100 2451
1
176 LI:1178352.1:2000SEP0871299232V 2111 2550
1
176 LI: l T 78352,1:2000SEP0871299865V 2134 2550
1
176 LI:1178352,1:2000SEP08688 T 09388 1049 1652
1 77 LI:814014.7:2000SEP0870998520V1 714 1006
1 77 LI:814014, 7:2000SEP087655145) 1 1 629
177 LI:8 T 4014.7:2000SEP082082708H 1 25 291
177 LI:814014.7:2000SEP083408374H 1 179 435
177 LI:814014.7:2000SEP083990460H 1 242 466
177 - LI:814014,7:2000SEP08399046086 242 839
177 LI:814014.7:2000SEP083943011 H 263 563
1
177 LI:814014,7:2000SEP087655145H1 394 956
177 LI:814014,7:2000SEP08337601 6H 480 740
1
177 LI:814014,7:2000SEP08g2337690 604 846
177 LI:814014.7:2000SEP08g2322463 630 891
177 LI:8140 T 4, 7:2000SEP0870156084V 754 1283
1
177 LI:814014,7:2000SEP0870154580V1 784 1292
177 LI:814014,7:2000SEP085526959H1 850 1082
177 LI:814014,7:2000SEP0870152121 V1 894 1327
177 LI:814014,7:2000SEP0870153959V1 912 1244
177 LI:814014.7:2000SEP08g4330870 1109 1306
1 77 LI:814014.7:2000SEP08g4599679 1186 1304
1 78 LI:1170b24,1:2000SEP086125643H 1 1 524
178 LI:1 1 70b24.1:2000SEP086128061 H 1 464
1
178 LI:1170624.1:2000SEP086125643F8 1 619
178 LI:1170624.1:2000SEP086128061 F8 1 649
'
T 78 LI:1170b24, T 6l 25641 F8 1 634
:2000SEP08
178 LI:1170624,1:2000SEP086125641 H 1 494
1
178 LI:1170624.1:2000SEP086125641 T8 113 678
178 LI:1170b24.1:2000SEP08612564378 124 681
178 LI:1170624.1:2000SEP086128061 T8 358 684
T 79 LI:1183 T 71,1:2000SEP0871003043V 160 478
1
1 79 LI.1183 1 71,1:2000SEP0871003556V 36 T 644
1
179 LI: 1 183171,1:2000SEP0871001329V 188 644
1
179 LI:I i83171.1:2000SEP0871003370V1 528 6i 7
7 79 LI:1183171,1:2000SEP0871002471 V 158 633
1
179 LI:1183171,1:2000SEP0871360733V 435 644
1
179 LI:1183171.1:2000SEP0871032742V1 412 644
179 LI:1183171.1:2000SEP0871004869V1 375 644
179 LI:1183171.1:2000SEP0871134190V1 171 629
179 LI:1183171.1:2000SEP0871004452V1 189 644
179 LI:1183171.1;2000SEP0871360725V1 582 644
179 LI:1183171.1:2000SEP0871002607V1 214 644
179 LI:1183171.1:2000SEP0871002247V1 403 644
179 LI:1183171.1:2000SEP0871001135V1 498 644
179 LI:1183171.1:2000SEP0871001 b 14V 441 649
1
179 LI:1183171. T 71003157V 577 644
:2000SEP08 1
__ __ _ -
21j
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component mart Stop
NO: IG
179 LI: I 183171. 7 i 001437V 1 580
T :2000SEP08 i
179 LI;1183171.1:2000SEP0871361965V1 35 678
179 LI;1183171.1:2000SEP0871004338V1 148 644
179 LI:1183171.1:2000SEP0871004890V 7 67 657
1
179 LI:1183171.1;2000SEP0871361650V 307 644
1
179 LI:1183171.1:2000SEP0871361538V 322 644
1
179 LI:1183171.1;2000SEP0871003761 V1 410 627
179 LI:1183171.1:2000SEP0871002546V 431 644
1
179 LI:1183171.1:2000SEP0871002044V1 551 1176
179 LI:1183171.1;2000SEP0871004513V 574 1140
1
179 LI;1183171. i 71003659V1 153 478
:2000SEP08
179 LI;1183171.1:2000SEP0871002417V1 202 655
179 LI:1183 i 71.1:2000SEP0871361432V 268 643
1
179 LI;1183171.1:2000SEP0871004044V1 431 644
179 LI:1183171.1:2000SEP0871361369V1 359 644
179 LI; l i 83171.1;2000SEP0871360768V 198 644
1
179 LI:1183171.1:2000SEP0871361912V1 249 630
179 LI:I i83171.1:2000SEP0871360744V1 486 644
179 LI:1183171.1:2000SEP087 i 360842V 333 644
1
180 LI:1093491.1:2000SEP08710bObb9V1 35 149
180 LI:1093491.1:2000SEP0870093523V1 220 682
180 LI:1093491.1;2000SEP0870564985V1 274 693
180 LI:1093491.1:2000SEP0870091980V1 288 696
180 LI;1093491.1:2000SEP085027241 H 311 580
1
180 LI:1093491.1:2000SEP0870091089V1 313 693
180 LI:1093491.1:2000SEP0870092803V1 312 693
180 LI:1093491.1:2000SEP085027241 F6 317 839
180 LI;109349T.1;2000SEP08~ 71057674V1 338 877
180 LI;1093491.1;2000SEP0870088231 V1 346 693
180 LI;1093491.1;2000SEP0870092382V1 345 694
180 LI:1093491.1:2000SEP0870089644V1 356 689
180 LI:1093491.1:2000SEP0870092039V1 379 693
180 LI:1093491.1;2000SEP0870089206V1 394 709
180 LI:1093491.1:2000SEP085867358H 1 452 724
180 LI:1093491.1:2000SEP085867358F6 452 876
180 LI:1093491.1:2000SEP08423930278 462 842
180 LI:1093491.1;2000SEP08416104478 522 946
180 LI:1093491.1:2000SEP08443955578 523 825
180 LI:1093491.1;2000SEP0871056905V1 569 1138
180 L!: i 093491.1:2000SEP0871060651 V 606 847
1
180 LI:1093491.1:2000SEP08g6990044 676 1121
180 LI:1093491.1:2000SEP0871060240V1 687 1119
180 LI:1093491.1:2000SEP0871060229V1 70 541
180 LI:1093491.1;2000SEP0871059449V1 35 549
180 LI;1093491.1:2000SEP0871058389V1 55 675
180 LI:1093491.1:2000SEP084161044H1 50 336
180 LI;1093491.1:2000SEP081527841 H 70 283
l
i80 LI:1093491.1:2000SEP081527444H1 70 270
___.. ___. . _ _ ___
_
~~.4
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
180 LI:1093491.1:2000SEP086349295H2 76 388
180 LI:1093491.1:2000SEP084439555H 1 1 280
180 LI:1093491,1:2000SEP084439555F8 1 606
180 LI:1093491.1:2000SEP0870090163V1 10 645
180 LI:1093491.1:2000SEP0871059365V1 i40 777
180 LI:1093491,1:2000SEP081739070F6 10 275
180 LI; i 093491.1;2000SEP087293047H 1 151 422
180 LI:1093491.1:2000SEP0871057653V1 204 870
180 LI:1093491.1;2000SEP0870089451 V1 203 695
180 LI:1093491,1:2000SEP0870090909V 10 509
1
180 LI;1093491.1:2000SEP0871606561 V1 10 661
180 LI:1093491.1;2000SEP081739070H1 10 247
180 LI:1093491.1:2000SEP0870093679V1 10 436
180 LI;1093491.1;2000SEP0870093745V1 10 485
180 LI:1093491.1:2000SEP0870090843V1 10 434
180 LI:1093491.1:2000SEP0870090268V1 10 464
180 LI:1093491.1:2000SEP0870091238V 2 i 8 710
1
180 LI:1093491,1;2000SEP0871607589V1 10 663
180 LI:1093491.1:2000SEP0870088285V1 208 682
180 Lf; i 093491, g5362171 24 T 29
i :2000SEP08
180 LI;1093491,1;2000SEP0871058989V1 24 553
180 LI:1093491.1:2000SEP0870092668V1 24 512
180 LI:1093491.1;2000SEP0871060482V1 32 345
180 LI;1093491.1:2000SEP084161044F8 50 622
180 LI :1093491,1:2000SEP0870091458V 10 446
1
180 LI:1093491.1:2000SEP0870089238V1 417 689
i 80 LI;1093491,1:2000SEP0870092828V1 10 416
180 LI;1093491.1:2000SEP0870093647V1 10 342
i80 LI:1093491.1:2000SEP08710b0092Vi 35 424
180 LI:1093491.1;2000SEP0871 Ob0687V1 378 796
180 LI:1093491. i 71059386V1 588 796
:2000SEP08
180 LI:I 093491.1;2000SEP0871060760V1 124 645
180 LI:1093491.1:2000SEP0870092525V1 10 363
180 LI:1093491,1;2000SEP0870090309V 10 418
1
180 LI:1093491,1:2000SEP0870088745V1 10 311
181 LI:04b515.5:2000SEP085546289F8 78 199
181 LI:046515.5:2000SEP08685576778 1 618
181 LI:046515,5;2000SEP085546289H1 78 256
182 LI:400171.2;2000SEP087309340H1 634 931
182 LI:400171,2:2000SEP082669002H 1 684 939
182 LI:400171,2:2000SEP087066680H1 942 1053
i 82 LI;400171,2:2000SEP084706031 Fb 1 507
182 LI:400171,2:2000SEP084706031 H 1 137
1
182 Lf:400171.2;2000SEP083371804H1 30 153
182 LI:400171.2:2000SEP082923902H 1 30 133
182 LI:40017 i .2;2000SEP084671 i 04H 30 i 37
1
182 LI;400171.2;2000SEP081494879H1 30 132
182 LI;400171.2:2000SEP0867562_ 08H 60 669
1
_
215
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
TABLE 3
SEQ ID Template ID Component Start Stop
NO: ID
182 LI:400171.2:2000SEP081393067Th 427 894
182 ~ LI:400171.2:2000SEP086756208) 1 436 i 096
182 LI;400171.2;2000SEP081393067H1 439 587
182 LI:400171.2;2000SEP081393067F6 440 606
i 82 LI:400171.2:2000SEP08488 i 236H 46 i 536
i
182 LI;400171.2:2000SEP085014037H1 485 726
182 LI:400171.2;2000SEP082179865H1 510 781
182 LI;400171.2;2000SEP084970021H1 597 867
182 LI;400171.2:2000SEP084706031T6 604 1045
182 LI:400171.2:2000SEP084659655H1 628 870
183 LI:330919.6;2000SEP087606569H1 1 469
183 LI:330919.b;2000SEP087606569)1 1 469
184 LI;219502.1:2000SEP08g2114615 1 367
184 LI;219502.1;2000SEP08g2241493 99 464
184 LI:219502.1:2000SEP082884433T6 128 430
184 LI:219502.1:2000SEP08g2112758 150 332
184 LI;219502.1;2000SEP08g2166952 203 515
184 LI:219502.1:2000SEP08g2167050 391 854
184 LI:219502.1:2000SEP08g6035041 395 729
184 LI;219502.1:2000SEP08g4186677 395 842
184 LI:219502.1:2000SEP08g4896038 402 855
184 LI:219502.1:2000SEP087170772H 1 734 924
184 LI:219502.1:2000SEP086608778H1 748 1260
184 LI:219502.1:2000SEP087343478H1 755 1340
7 84 LI:219502.1;2000SEP087587604H i 780 955
216
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
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CA 02419943 2003-02-20
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<110> INCYTE GENOMICS, INC.
JACKSON, Stuart
LINCOLN, Stephen E.
ALTUS, Christina M.
DUFOUR, Gerard E.
CHALUP, Michael S.
HILLMAN, Jennifer L.
JONES, Anissa Lee
YU, Jimmy Y.
WRIGHT, Rachel J.
GIETZEN, Darryl
LIU, Tommy F.
YAP, Pierre E.
DAHL, Christopher R.
MOMIYAMA, Monika G.
BRADLEY, Diana L.
ROHATGI, Sameer D.
HARRIS, Bernard
ROSEBERRY, Ann M.
GERSTIN, Jr., Edward H.
PERALTA, Careyna H.
DAVID, Marie H.
PANZER, Scott R.
FLORES, Vincent
DAFFO, Abel
MARWAHA, Rakesh
CHEN, Alice J.
CHANG, Simon C.
AU, Alan P.
INMAN, Rebekah R.
<120> SECRETORY MOLECULES
<13.0> PT-1184 PCT
<140> To Be Assigned
<141> Herewith
<150> 60/229,751; 60/230,016; 60/229,749; 60/229,750; 60/229,747;
60/229,748; 60/230,583; 60/230,517; 60/230,599; 60/230,514;
60/230,988; 60/230,518; 60/230,515; 60/230,610; 60/230,597;
60/230,505; 60/230,519; 60/230,595; 60/230,990; 60/230,865;
60/230,989; 60/230,596; 60/230,864; 60/231,163; 60/230,896;
60/230,897; 60/231,832; 60/230,951
<151> 2000-09-05; 2000-09-05; 2000-09-05; 2000-09-05; 2000-09-05;
2000-09-05; 2000-09-05; 2000-09-06; 2000-09-06; 2000-09-06;
2000-09-06; 2000-09-06; 2000-09-06; 2000-09-06; 2000-09-06;
2000-09-06; 2000-09-06; 2000-09-06; 2000-09-06; 2000-09-06;
2000-09-06; 2000-09-06; 2000-09-06; 2000-09-07; 2000-09-07;
2000-09-07; 2000-09-07; 2000-09-07
<160> 369
<170> PERL Program
<210> 1
1/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
<211> 964
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:983076.3:2000SEP08
<400> 1
aatggaactt aatgtctatt cctcttttct cttttctgtg ttaccaaagg gatctagagc 60
cttatgggta ctacttagaa aatgagcact catacatata caatatctgg aaataccttg 120
aaagtaatca cgaatactcc ttctgtttca cttccatact gctgaattgc ctcttcatct 180
tctgtcacca taacaattgg catcctgtcc tggcagtgat gatagtacca aacagctgcg 240
ttgtatatgc tcctggtctg ccacttctcc atggactctc ctctttcccg tggcagatag 300
cagcattgct ggaattcatt agcaaagaga atgcaatcat gacgcgcatc cttcagcagg 360
tttcgcagtt tgttatactg tctgtgacac ggaggagaaa aataactcta acaaagcagg 420
tactcatcca ctgctatctc ttgctaagga tcaaaaaagg atggggtatt ttagagccaa 480
agattgatta ctttaaacct gaagaacatg tatttatatt ggtctcagtg tgctcagtcc 540
tatcgggctg ctattgtttc tcgattttgc ctgctagaag gtgcttgggc agggcctagt 600
taattagttt gcagatcaat taaactcctt ccctctcctt tccccttcag agacttccag 660
ccattttatt atgtaattat accaccttgc cctgcctagc cccttcttca aagcctgagt 720
gcacggaggc aagcagagag tttagcactt tatttttgga cctcactttg ttctataaat 78D
gtcattaagc attaatacat ttgacttttt gctggcagcc ctagacaaga acatgtaagg 840
taaataagtt tggggattta tctgtgaatt ttatttatgc agccaccctg atttacataa 900
atgagagcta gcctcatctg tcagacaact aacagaaagt tttaaggttt tgtccatcaa 96D
gaga 964
<210> 2
<211> 790
<212> DNA
<213> Homo sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:1382987.7:2000SEP08
<400> 2 ,
ggctgagcgg tgcccctccc accggcccac cagaggcccc ggctgttagc tgcatgtata 60
cttgtgttcc aggcttgcga actaaaattt cattggttaa ggatgtgaga agcgtcagcg 120
agatgtgtcc ttaacatctc tgaattcagc taaggtatgt gccttgtgaa gaaatctccc 180
acttggaaca cagccccaga agctctccat tcccagagtg gagttggaag caccacaagg 240
cttgctcagg gcaggcggag gagggccata ccatctcaca tccagccagc tcatggttcc 300
aacgggcttc catcaggctg tggagaacct gagggtagat ggtcaggatt ctggaaaagt 360
tctgcagacg gcttctgaaa cgctggtagg caaggtgcac ccacggtaag gaaacctctt 420
cctcgggcgc ttctgacgcc gctttcagtt tcctagtgca ggaccacaga gccatctgca 480
gaaactggga ttcaaacaag cttttagaat atatttttaa attacaaaaa tggtatgttt 540
tccccgtggg aaatccgaac tattggaaaa ccatacaaag aggaaattag aaaccatgtg 600
tgctattctg tcccgtttgc ttctgtccag ttttctatct gcctctcctc ccacgttaag 660
gtcaccgtgt cgtctgggct ttttgccact gaacatgaca tcatgaactt tccctatgac 720
tgagtatact tgaaacacag tggttttaat ggctgctcaa taacccatca tacggccata 780
ctttaatttc 790
<210> 3
<211> 291
<212> DNA
<213> Homo Sapiens
2/236
CA 02419943 2003-02-20
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<220>
<221> misc_feature
<223> Incyte ID No: LG:235557.15:2000SEP08
<400> 3
cctcttttta aaatttctgc taacttgata ggtgaaaaac agtagtttat ttaatctgca 60
tttctctgta tttcagatgc atgctgcctt ttttgtaaat tctagtcttc tttttgttca 120
tttacctttt gggatcttag tttccttatg aatatttgag ctttttagta tgtaaataat 180
aatcatgtgt cattctgtta cagacatact ttccattgtt gctcttaatt ttgtattaac 240
actcagatgc cctgagtctt tcagaggttt ttattttttg agaccgagtc t 291
<210> 4
<211> 805
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:018494.1:2000SEP08
<400> 4
atagatgctg aaaggctaat acacacatat gcacatgtat ttgattgtca aaggtatatt 60
cttaaatttg ggtattattg aaaatatttt ccatgccttg gtgctagcat ataagtttgg 120
aagtttgcca acatcacaat tcatcttgaa aagagctttt ttccctccta ccacatacac 180
cattcttagg gagcaatgag gtaacaggtc tgtgttgtct agatctttgc tttttatccc 240
cctatcagtc cagggcatat actaacctgc aaactgattc tgaatcagga aggtggtaat 300
caataagtat tctggctggg aaagaccgtg ggcccaatga tcaaagtctt cttggtgctg 360
ttcattaatt cttgtgcctt ttggcttgtt ttctagagtt tctgggcttt ggctgctgat 420
actgcctttc ttagactgta atttttatct gcatgcccag tttctgacct atcaacttgg 480
gttttattgt gcactctaac tgagcttgtc ttcataattt tctgtttatt gccctgggct 540
tggatatgtc tcaagacact catgtgaatc atgccacccc aaatcctggc ttatcaagtc 600
ccagactata aattatgaac tcccattagc ttggtactaa catatacttg atgtaggtat 660
ttatggactt gatgatccaa gaatattata ttcttcaaaa tggttaagct ccatggagtt 720
agatgactac acttaatgct attaagttga acttttgaat gtcaactaat ttgcaatcaa 780
ttaaagatac atatgcctag aaaaa 805
<210> 5
<211> 1877
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:980494.1:2000SEP08
<400> 5
cgttcagtgg ttcccggcac gctcagggtg cagtgtaggg tctgaatgtg tgtattgcgg 60
gggtaggggg agtggtgtca gttctaggcc acaggaattc gtggtctggc cccagaggtg 120
cggtgttttt ggccgggaag tcaggcagaa gtctgcagcg tgcaactcgc agcggggcgt 180
gtgtgtgcgt gtgtgcgcgc gcgtgtgcat gtttccggcc cggggtcgcg tgtgtggctg 240
cagcctgtct ccgtgacaag aagatccagg gatctacggg cggcacgggg aagggattaa 300
ggggaacata cttccctacg tcttttctgc ctccttttcc cagggtagca ttctttgctt 360
atccaatgat gcctctttct aacctctccc tctccactta catcccaaac ctgatgcggg 420
tcttctctga ttcggcactc taaggaggac tcagccgccg gctgcgacca ccgtggactc 480
cctctatgga agggaatcct gggagtcccg gccgtcagag gggttcccaa atccaaaggg 540
tccatctcca aggcgtcact ccaggggtcc gcggttcact ttgctgcccc ttgcaggtga 600
3/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
tgctgtgatt gttgcctgcg gactgggaag cacgatggct ccaaaaggcc agcggggccg 660
agaagcaggg acagagacca gcatttaagg gatctctggt gggggcctcc ctggagttct 720
ccacatgtaa atatagcgaa aaaagaaaac actacgttac tagaataaac acgtcctaaa 780
atttctgtgc aaaaacacct ttttgaacag caaagatttc acccaccaaa acaaggaagc 840
aagaagcttc cacagtgtgc aggatccgaa acaggaccgg cccggcgttg ggtgagagtg 900
ggggaggggt tgtcggcttc tccatccagc ttgccatgtt ctgtcgtcgg tttcacattt 960
tcagagagac tttaccagat gcaggaaatg aggatgaagg caactctgga aggatgcaaa 1020
gaagggagag caagcactgt acctactgtc tgctgtctaa gagggctggt ctcataacta 1080
aaaggtccag tccctgagat cttgtgtgca accgtcagaa atagttcagg acatcaaatt 1140
gcacagagaa gattcagatg aagggaaaca agaactctgt ggaaaactgt tggggacagt 1200
ggcacgagtt gtcttgcaag gtgatgtctg tcccgtggag ggacacaaga gcgaggacag 1260
catggttcta atggccaggg catgtcttct tattccatgt gacagtggct ggctgagttg 1320
ctagtacgtt tttgaaatat accctttgaa atatgttgat tattctggac atctgtaagg 1380
tgggagattt gaataaaaag acttctgctg tcattattgg cttttatggc tatgattttt 1440
ttcagtcatt attataaatc atgtgaaggt aaaataaatg atcattttag ccattctgtt 1500
ctgcacagaa tgctgttcaa ataaaacatc atctgatatg tttgcctgta ttgttttgtt 1560
tgggggtcac ggaagaggga aagtcggcca aattgattag ggtatcatat aaaagcagag 1620
attaggtccg ggtagatctt ttgagtgtgt gcaggaaaag gcttcattat agaatgctaa 1680
atttaatgtg agatgagaga atcttttact cccgatctcc tgcatttgaa atacaaagga 1740
ttgtaccctt gggctggttc ctggtcctta agtggaagcc taactggaca ctttttttct 1800
ggaggattgt tccctaaatc ctgggaagct ttagaaaata gtcaatggaa tttttgttgc 1860
cctaagataa agtcatt 1877
<210> 6
<211> 1273
<212> DNA
<213> Homo sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:984457.2:2000SEP08
<400> 6
tcactacagg tcaaaaccaa gagagaatgt gtagttttca aggtcttggc cagaaccttt 60
aggaaagaag acctgtttat acattgaagg aagaaaagaa ggaagcagtt gccttccgga 120
gggggctctg agagaatcta gcctcccctc tgtcctattg gagcaaagat tggagtgagt 180
gttgccacca acaggatttt atcgtttgac tccaatacct gaaattctga cttctctcct 240
gtgcttcaat gagaatgata aattatccta gcaaaggggc ctctggagac catcttgttc 300
cagcctctga agacagttga ggagatcaag cccagcaatg gtggcagaat cttactccac 360
agacttcagc agactagtca tttcaatacc caaagaaaga caagtgacag gggcaatgga 420
tctcaggctc tgagataagt atatcagatg acactggtgg ctctaaggat attgcaatta 480
agcagctacc tgtagccagg tattctgctg ctcttggcct tttcccacgc atcgtctcgt 540
gtcttctccg aaagaccttg gaagataggc ctggaagaga ctgttgatgc cactttgaag 600
aaaagaacac tgagaactag aggagggaac actttgccca agattactca caaagccaag 660
acccagagtc cagcttagag aatagagttg ttcaggctgc caattgcaag ctcattcctc 720.
tacctcatac ttcctctgag gattttgaca aaatggatta attgggtgag ccttggagac 780
atgtgggaaa cacctgcaga cacaaaatga gtagtcatcc tgtctccctt tcaataggga 840
tctgaacagg tgttttgata cttgaaagat gtgcatgtca agtgagggtt tctttctgcg 900
atgttcaact ggaactctcc catcagtagt tacaattaga aatacctact gatggttagt 960
ctgaaggcca ttctcatggt cacctataca gtgtgtttcc ctgtgagcta gcagacacaa 1020
tgaccaggaa aaaacctatg aattccattc ttaggtttcc cagccaattg ctcccttctg 1080
ctttagaagt gactaggtac tgagagtaca aacactccca ctttataatg aaggcgtcat 1140
gtcacccctt cctttacagg tcctggggtc caggagaccc agaatgaagg tgtcagttgg 1200
gcatgaagtg ttatttagtg tccattcttg atccttctga gcacctacag ctggaaacta 1260
agcagatact ggt 1273
4/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
<210> 7
<211> 1663
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:406758.1:2000SEP08
<400> 7
agcctgacac atgaggtctt ggttctatga cttgaccaca tcctgagagc aaggtggggc 60
cctcgactgt cttgtatggg ccccaagcct ttcttgctgt gaacagcttc attctcagtc 120
ccctttcctg gctcaaaaat cgagcctagc aaaagaagag gcacgggaag gaaacgcccc 180
tccctcctcc tgcaggcagc cactcagcct gtgccttcag cttggtgccc tggtgatggt 240
tgctatggag atctaaagat agagcaggag tcaggagacc tgggtccctc tcccagcttg 300
tcccactgag cggctgccga tcacggtcca cccaccccag gaacctggca cccctatccc 360
aggaacccag tgcccctggg cagcgcactg cagttaatga attctgcctt caacagcctg 420
ggctctagcg tgctgcacca gcttgggcta gctacaggca gtcccagaat tggaatatta 480
agccatggaa tcttagagtt agacttttat agtcatagaa atgtactctt caccatatca 540
aagctgaaag ttcaggactc taagaaacat acagtgtgac agacttatcg tatacacggg 600
aaactgaagc ccagtgagac taaatgactt gcccagggta aaacagtcca tggcagagcc 660
agcactagaa gcctcctagc ttcaacctca tgtgccaatt ctgcatcagt gattttcgaa 720
ctgcgctcta caggaagcaa gggttctttg catagcagca gtgaaaggac agctccagat 780
ggatccccag tacccccttc aaccagagca gctgcatttt tatcccattc atatattggg 840
gttttgaatt atttttattt gtacaaaagg attttcctag ccactaaaaa aaatggcaac 900
aaatctctct~gctgtgtcac atttaataaa taattgtgac acatagctgg ggggtggtgc 960
tggaggtttc agaccgcctc acaggcagag ttccagggtc agagagcatg gagtactgtg 1020
tgtgaccttc ttccagaagc acgggcacag gagcatgtta aaggccctga aaagaccagt 1080
ggcaaagaaa ctcctttcat tttgtttaat ccagagtttt tcaagcttat ttaactatag 1140
aacacttttc tgtgggtttc gtagcctctt tctagcacag tatagtgagt gggaaaggcc 1200
ctgactgaga gtcaaagatt ctgagcagga accatgatct cttcctgcct agtatgtgac 1260
ttgagcaaga cacttttcct ctccccaggc ctctgaggtc tgcgagtttt cctccaccct 1320
ttcctctccc tcagtttgca catcctattg actcagctcc aaatagcggg ggcctgtcag 1380
gagcctgact ctgttcagac accagggaaa caacagcaca ccaggcagat gcagttatgt 1440
ccatcgggga acttacagcc tagcaaggaa ggcagcaatt caacctgtaa gtctaaaagt 1500
gatgggtgtc atgaaaaggt aagtgcaggg tgctgggaga gtggttggca ggggctttag 1560
cctagtctag ggagagccca ggggcacacc actgccagcg gggatagagg cttcaaaagc 1620
tttgaagtca ctcatattaa gagtgttgcc ggacacggtg get 1663
<210> 8
<211> 294
<212> DNA
<213> Homo sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:902957.17:2000SEP08
<400> 8
tggatttacc tccactttag taataatgac ataacacatt ctaatgctaa aaaatattca 60
ttattactac actttggctt gggagaggaa ttattttaaa tagacattgg tactttttga 120
acttgatagc taaagattct aaaatgcatg ttttatacta agttttaacc agtcaggaaa 180
attttatgta actagtgata gtttattttt ttgtatgaat tttgtttagg ctgcaatgtt 240
tagcttttgt taactcctca ctcttgctgt cttaagttca ttactatgtt taat 294
<210> 9
51236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
<211> 933
<212> DNA
<213> Homo sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:333179.1:2000SEP08
<220>
<221> unsure
<222> 652, 657, 670, 821, 828, 853, 922
<223> a, t, c, g, or other
<400> 9
ggaaggtgac tgtgaggaag gtgtgtgtcc accgagcact tggattcagc ttcttcattt 60
ccaacatgga agaaacttac accgactccc tggaccctga gaagctattg caatgcccct 120
atgacaaaaa ccatcaaatc agggcttgca ggtttcctta tcatcttatc aagtgcagaa 180
agaatcatcc tgatgttgca agcaaattgg ctacttgtcc cttcaatgct cgccaccagg 240
ttcctcgagc tgaaattagt catcatatct caagctgtga tgacagaagt tgtattgagc 300
aagatgttgt caaccaaacc aggagcctta gacaagagac tctggctgag agcacttggc 360
agtgccctcc ttgcgatgaa gactgggata aagatttgtg ggagcagacc agcaccccat 420
ttgtctgggg cacaactcac tactctgaca acaacagccc tgcgagcaac atagttacag 480
aacataagaa taacctggct tcaggcatgc gagttcccaa atctctgccg tatgttctgc 540
catggaaaaa caatggaaat gcacagtaac tgaataccta tctcatcaaa tgccagaccc 600
tagaagactg ttgcttcttc ttctaccagt gggttctcat tttcctccta anctaantat 660
agaatggtan actccctgtg actttccaaa ctgacaagca cacttttttc ctcccccctt 720
gaatcctcat ttaatgcaag aaccctcata ctcagaagct tccaaataaa cctttgatac 780
agattgctta atagtttggt caagtacctg cagccccaag ntcagagntc atgtccaatt 840
ccacattaat ganccataat gatttaaagt tttcttaaag gttctaactg aaatagacag 900
gaaacttggg ttagaaaatt tnaaagggaa aaa 933
<210> 10
<211> 2280
<212> DNA
<213> Homo Sapiens
<220> .
<221> misc_feature
<223> Incyte ID No: LG:406568.1:2000SEP08
<220>
<221> unsure
<222> 445, 1360
<223> a, t, c, g, or other
<400> 10
cagcctgcca cttgcctccc tgcctgcttc tggctgcctt gaatgcctgg tccttcaagc 60
tccttctggg tctgacaaag cagggaccat gtctaccttt ggctaccgaa gaggactcag 120
taaatacgaa tccatcgacg aggatgaact cctcgcctcc ctgtcagccg aggagctgaa 180
ggagctagag agagagttgg aagacattga acctgaccgc aaccttcccg tggggctaag 240
gcaaaagagc ctgacagaga aaacccccac agggacattc agcagagagg cactgatggc 300
ctattgggaa aaggagtccc aaaaactctt ggagaaggag aggctggggg aatgtggaaa 360
ggttgcagaa gacaaagagg aaagtgagga agagcttatc tttactgaaa gtaacagtga 420
ggtttctgag gaagtgtata cagangagga ggaggaggag tcccaggagg aagaggagga 480
agaagacagt gacgaagagg aaagaacaat tgaaactgca aaagggatta atggaactgt 540
aaattatgat agtgtcaatt ctgacaactc taagccaaag atatttaaaa gtcaaataga 600
6/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
gaacataaat ttgaccaatg gcagcaatgg gaggaacaca gagtccccag ctgccattca 660
cccttgtgga aatcctacag tgattgagga cgctttggac aagattaaaa gcaatgaccc 720
tgacaccaca gaagtcaatt tgaacaacat tgagaacatc acaacacaga cccttacccg 780
ctttgctgaa gccctcaagg acaacactgt ggtgaagacg ttcagtctgg ccaacacgca 840
tgccgacgac agtgcagcca tggccattgc agagatgctc aaagtcaatg agcacatcac 900
caacgtaaac gtcgagtcca acttcataac gggaaagggg atcctggcca tcatgagagc 960
tctccagcac aacacggtgc tcacggagct gcgtttccat aaccagaggc acatcatggg 1020
cagccaggtg gaaatggaga ttgtcaagct gctgaaggag aacacgacgc tgctgaggct 1080
gggataccat tttgaactcc caggaccaag aatgagcatg acgagcattt tgacaagaaa 1140
tatggataaa cagaggcaaa aacgtttgca ggagcaaaaa cagcaggagg gatacgatgg 1200
aggacccaat cttaggacca aagtctggca aagaggaaca cctagctctt caccttatgt 1260
atctcccagg cactcaccct ggtcatcccc aaaactcccc aaaaaagtcc agactgtgag 1320
gagccgtcct ctgtctcctg tggccacacc tcctcctccn cgggactcct ccactcccag 1380
agaaaaagct cattaccaga aacattgcag aagtcatcaa acaacaggag agtgcccaac 1440
gggcattaca aaatggacaa aaaaagaaaa aagggaaaaa ggtcaagaaa cagccaaaca 1500
gtattctaaa ggaaataaaa aattctctga ggtcagtgca agagaagaaa atggaagaca 1560
gttcccgacc ttctacccca cagagatcag ctcatgagaa tctcatggaa gcaattcggg 1620
gaagcagcat aaaacagcta aagcgggtgg aagttccaga agccctgcga taaaaacatg 1680
atctttagaa gaggatgcag aactgttcag tggtattaca tgaaatgcat tgtgagatgt 1740
ttctaaaata ccttcttcaa ttcaaaatga tccctgactt taaaaataat ctcacccatt 1800
aattccaaag agaatcttaa gaaacaatca gcatgtttct tctgtaaata tgaaaataaa 1860
tttctttttt atgtcgtgag atttgtattg gcaagaagca gttaatttaa agatgctctt 1920
cctatctgtg gatgtgttgg taactccgag ttgtaatgag ttcatgaaat gtgctgttat 1980
ttttgtaatc tcaataaatg tggattgaag ttttttccct ttttttaaag ccaaactaat 2040
atttttctgt gacttgatac atctgtcaga tttttgtaat ctcgataaat gtgtattgaa 2100
gttttttccc tttttttaaa aagccaaact aatatttttc tgtgagttaa tacatctgtc 2160
aggtgtgtat gtaacattac tggacattaa aaaaaattat tacattctca cccaaaaagg 2220
gtttgggtcc taagcatttg cctttctttg tttcctcttg ttcaagaaaa tctgattaga 2280
<210> 2l
<211> 609
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:353203.1:2000SEP08
<400> 11
cgagagggcc ggagcagccg cagtcccggc gatcccgccg ccggtcgcec tcatacttcc 60
tcgtcactgc cttcgtttct ctttccaaca atcaagatga gccgtggcgg tagcgcgggt 120
ggtggtcaaa gttctctggg ttacctcttt ggaagcggtg agccccccaa accagcagtg 180
gcaccagctg taagtgctcc acctgctgct gcaggtgctc cacctgctga gaaaccacct 240
gctgcaaagc ctgatggcac cagtcagatt gctgctgggg ttaccagcca aaccaataac 300
taccacaggg ccgacggtca gaacaccgga aacttcctta cggaccgtcc ttcgaccaag 360
gtccacgctg ctcctggcgg tggctcttcc ctgggatacc tgtttggtgg caactgatgc 420
cgctcaccaa gctatcaccc cccactgtcg ccgcatgctt tttctgaatc gtgaacagac 480
cgttgtcatt tcatgtgtac ccaaacctcc cgtcccgcgt gggcccgtaa gctgcgccta 540
gatttcaggg ctaatcgtgt gcctgaaccc tcatcataag tcgtctctgt acccagcttc 600
tcgtttcgt 609
<210> 12
<211> 504
<212> DNA
<213> Homo Sapiens
7/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
<220>
<221> misc_feature
<223> Incyte ID No: LG:061277.1:2000SEP08
<220>
<221> unsure
<222> 475
<223> a, t, c, g, or other
<400> 12
attttttgat agacaccatt gcaaaacaac ataaagctgt tttgctcaca ggagagcagg 60
gaactgcaaa aactgtcatg gttaaggcct atttgaaaaa atatgatcct gaagtacagc 120
tatccaaaag tctaaacttt tcatctgcca cagaaccaat gatgtttcag agaacaattg 180
aaagctacgt ggataagcga attggaagca catatgggcc accaggaggg agaaaaatga 240
ctgtatttat tgatgatatt aatatgcctg tgattaatga gtggggagat cagataacta 300
atgagattgt gcgacagatg atggaaatgg aaggaatgta cagcttggac aagcctggag 360
acttcactac tattgttgat gtgcagctca tagcagcaat gatccaccct ggaggtggtc 420
gaaatgatat tccacaacgt ttaaaaagac aatttactgt gtttaattgt acatngcctt 480
caaatgcttc aatagacaaa attt 504
<210> 13
<211> 470
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:170666.1:2000SEP08
<400> 13
gtgaaacaca ttcatcttca tcagaaagag cctcatcttt aattgcataa aacagaaagg 60
ttggctcgca cttccctcgg ttggtgactc cgggcgcgtc gaagatcttg tacctgtcgt 120
tcgcagtggc tcactttccc aagccatggg cagcaggaag aaggaaattg ccctgcaggt 180
caacatcagc acccaagagc tttgggagga aatgctcagt tccaaaggac taactgttgt 240
tgatgtctat caaggctggt gtggcccctg caaacctgtg gtgagcctct tccagaagat 300
gaggatcgag gtcggcctgg accttctgca ctttgcatta gcagaggcag atcgtcttga 360
tgtcctcgaa aagtacagag ggaagtgcga gccaaccttt ctgttttatg caattaaaga 420
tgaggctctt tctgatgaag atgaatgtgt ttcccatgga aagaataatg 470
<210> 14
<211> 984
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:311197.1:2000SEP08
<400> 14
gctctccacg tgcgcgactg cgaggctgga cgctacgggc tcctggaaag gagttaagat 60
gccaaatttg atatctggag cacctggaac cttgcagagt gttttgaaga ggaggttaca 120
atgaaaaaca aaacaaaaaa gaaattttgc tctgcaagtc tactgacaag tcccgtttcc 180
ttcccacatt ccaatctatg tctgcctttg tgttcagcag acaccagcat ttgccacaat 240
gctgtcatcc actgacttta catttgcttc ctgggagctt gtggtccgcg ttgaccatcc 300
caatgaagag cagcagaaag acgtcacact gagagtatct ggagaccttc acgttggagg 360
agtgatgctc aagttagtag aacagatcaa tatatcccaa gactggtcag actttgctct 420
8/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
ttggtgggaa cagaagcatt gctggcttct gaaaacccac tggaccctgg acaaatatgg 480
ggtccaggca gatgcaaagc ttctcttcac ccctcagcat aaaatgctgc gccttcgtct 540
gccgaatttg aagatggtga ggttgcgagt cagcttctca gctgtggttt ttaaagctgt 600
cagtgatatc tgcaaaatcc tgaatattag aagatcagaa gagctttcct tgttaaagcc 660
gtctggtgac tattttaaga agaagaagaa aaaagacaaa aataataagg aacccataat 720
tgaagatatt ctaaacctgg agagttctcc aacagcttca ggttcatcag taagtcctgg 780
tttatacagt aaaaccatga cccctatata tgaccccatc aatggaacac cagcatcatc 840
caccatgact tggttcagtg acagcccttt gacggaacaa aactgcagca tcctcgcatt 900
cagccaaccc ccccagtccc cagaagcact tgcggatatg taccagcctc ggtctctggt 960
tgatacagcc aagctcaatg cagg 984
<210> 15
<211> 1004
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:220655.4:2000SEP08
<400> 15
gtcttggtta ccgcggcgtc aggcggggga ggcgctcaca gctggcctaa caggtgaagg 60
acggcgcagg tgggcggagg cctgtccgcc aaggaccgcc gggtccgcct gtgcgctggg 120
aaggcccgcc ccctagcggc cgatctttag agtacggagg gcagccccca aaggcctccc 180
atccctgtcc ccgcccctgg gcccctggac tcactttgat ctcgatctgc tcctccatct 240
ccttgctctt catggctgcg tactccttct ccagcctgca gatcgggagg gccccgtggt 300
tactgtcatg ccctcggggg tccccccttc ctaccagtac cctggttgcc cagcaggcac 360
cgacctcttc atcttcttgg ggttgtactt gacctggtag gctttgagga ccagcttgtc 420
cgggcagctg tcgaactggt gggggatcac tctctggaag tactgtgggg acacagccgg 480
tcacctggtc accaacatgc aggccctgac caccgggggc cgcccgagcc ctgttgccct.540
ggaggggggc aggaacgggt ctgcccgggg tgtcgccatg tcctcaacgc cgtgtcctca 600
gtgtttgtgg agcaatggaa agactgagcg tgaggggagg gaatggggga ctgcctgcag 660
ccaaagcctt agtttccaga agaaaagctg gcagatgctg gatgtggtgg ctcattcata 720
taatcccaat gatttaaaag cctgggacat cccctccatg tccagctgca tcagctccgc 780
ctggttcacc tgcagcaggg cgaggcccac tcggaacacg atctccagcc cctcatacat 840
gaagatgtca aagacccggg tggcgacggg gagtgggaag gtggtcagga acagtgtgag 900
gaaccaggac gaggcataca tggatgtgtg gaagctttgg gaacggaagt gggtgttgag 960
gtctgggagc tgctcctgca gccatgtact cgaactgata gatg 1004
<210> 16
<211> 868
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:1001893.1:2000SEP08
<400> 16
caggcctggt cagacatggg caaggagcac aagtcagaga agcacctcta caaagagtat 60
gtggaaaagc ccttgaagct ggtcctcaaa gtaggaggga aagcagttac tgagctctcc 120
acgggcagtt tggagcactg ctccagcctc ttcaaagaca aaaacaatca tgacaaacac 180
aaggacagaa agtggaaaaa gagaaagaaa ggagagaagc cagttctggg ggaagaaaag 240
gggagaaagc agaattgaag aggacaaaaa taagcgagat caagaccatg tggagaatga 300
ggcagaaaaa gacttccagt gtcatgcccc tatgagatta gacttgcctc ctgagaagcc 360
tctcacaagc tgtttatcca aacaagaaga agtagaacag atgtcccttc aagatgtttg 420
9/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
tttgtttgag acagggtatt gctctgttgc ttaggctggc atgcagttgc acaatcatat 480
gtgcaattgt agcttgttga gcacccgatt ttattgctgt ttggggcagt acttgtagcc 540
tgcatggacc ttgggagact gaacaaaggg ggtgtaacgc gggaataaaa gacaaagaca 600
aaagagtata attggaagaa ggggtcgggg gcaccttgcc tctagtggac agggtccttg 660
agctttttca gccctctgaa tgtattaggt aaaagagata acgagaaaga gagggtgatt 720
gttgagtaat tatcagtcag cctgtttggt tcacagcagg cttgtgagac tgcatccttc 780
gaacaatagg tgcaggcttg tgagactgca tccttcgaac aataggtgct agatttccca 840
gtagataact tcaaggagcc cagcgcca 868
<210> 17
<211> 2545
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:004335.1:2000SEP08
<400> 17
ctctcttctc gagctctctt ctcgaggttc tagatcgcga cggcggctgc agctggagcc 60
aggcgctcgc ccgtccgccg gttggctcgc cgggacctcg cgcaccggcg gcagagtccc 120
ttgcgtggat tggcaagcga cgccccacct gccccgagct caccattttc tttcgcgctg 180
gctgcagctg acccggcgaa gggagccgac cgggccctgg gctggaggta aaaccccacg 240
gaaagaacat gaggttccct tggaaatcat tcaagaggaa gatggaaggg gctgtttaga 300
agagcttaaa agctacaggc tgtaagactg gggcctgagt gctggcagtg gaaaacactg 360
ttgggctgtg atctctccct gaaaagttcc aggtgccttt ttccttcctg caaaagaaag 420
gaagcgaaag agaagaccat gtccatagcc ctgaagcagg tattcaacaa ggacaagacc 480
ttccgaccca agaggaaatt tgaacctggc acacagaggt ttgagctgca caaacgggct 540
caggcatccc tcaactcggg tgtggacctg aaggcggctg tgcagttgcc cagtggggag 600
gaccagaatg actgggtggc agtacatgtg gtggacttct tcaatcggat caacctcatc 660
tatggcacca tctgtgagtt ctgcaccgag cggacctgtc ctgtgatgtc agggggcccc 720
aaatatgagt atcggtggca ggatgatctc aagtataaga agccaacagc gctgccagct 780
ccccagtaca tgaaccttct tatggattgg attgaggttc agatcaacaa cgaggaaata 840
tttccaacat gcgtgggtgt tcccttccca aagaacttcc ttcagatctg caagaagatc 900
ctgtgccgcc ttttccgggt ctttgtccac gtctatatcc accacttcga ccgggtcatt 960
gtgatgggtg cagaggccca tgtcaacacc tgctacaaac acttctatta ctttgtcaca 1020
gagatgaacc tcatagaccg caaggagcta gagcctttga aagaaatgac gagcaggatg 1080
tgtcactaat gctccacctc accctttgga agaaaggaaa gctgtttcct cctggtgccc 1140
tgagcgggca ggaggtggac caccctggct gaaatgacac acctactccc aggaacagca 1200
gaggtggagg caagcagtga ctcctgagag acattcccca ctcactttgt gtgctcttaa 1260
ccttctgagt gctgctagcc cagacctgtg gacgaggcag accacaacgt gaaagaagga 1320
ccagcccctt gaccgttctg gctggggaat tgtccacgag gaagcctctg cacttccaca 1380
catggcacag ttctgcctgt gacctgccgc ctaagcttta ctggaattca ggttttgaga 1440
ctgagatgcg tgttcgtatt tttccactta tctgtcttgt cagctggccg acttctctgt 1500
gattggtttt ttaagtgccg ggtgaatttt ggacctctgg atgtgcagca agtttttatg 1560
caataagcct tcctttcagg tctctaaaag ctcctgctct gatctgtggt ttaacactgt 1620
gcagggctgt ggagctctga gagacctgaa cccctaccca tcccctgcac ctccctactc 1680
tccctgccga ggcgtccata gcatttccct aataaatagt tttatcaggg acactccatg 1740
gggtggcttg tctctgcccc aaacagggtc ttcacgttct aactgcaggg aagagactgg 1800
ttactagcgt aaagctgaca agttaccagt tcacctgatc agaatgtatt ttttataacc 1860
accatcattc cttgtctctt cagtggaaga tattctttcc tgtttcccag aagagagaaa 1920
taaaagtcct aaataactaa gaatgatcag cgggagcgtt gggcatgatt actgcagtgt 1980
ttgttcttta ttaaagacag ggagtcgtgg ctgtctctac ataatactaa catttcagtg 2040
aaaaaagtac agttggttat ttggtacgtg tagatttaac acccagacgg ctgacttgta 2100
accctcccca ctagccttct gagcatttaa acccagccgt cattctctcc ccactccacg 2160
ctccccactc ctctgccacg ttatgttacg gcacaaaatt aatttctgcc cctgtgattg 2220
10/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
ggccacggtt gccaaggtaa cagtggagct ggagctaact tccttctttc atcctttcca 2280
atttttctat tccagaagac agtcagaata atgcattctg tactacatcc tgccttttga 2340
aacctaaatg agttttcgtt gatgaaatgt tgccttctct gattcattca caaaactgtg 2400
gtggttctga ccacctgtga cgaggggggt cataatactt ccagtgatcc ttttaattta 2460
gcaaaatatt tgtcggtgga gggaagtaga taagaatgta ttagtgtatt ttaaagtaat 2520
aatgattaaa gaataactaa atgaa 2545
<210> 18
<211> 393
<212> DNA
<213> Homo sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:213092.6:2000SEP08
<220>
<221> unsure
<222> 30
<223> a, t, c, g, or other
<400> 18
catccctggc caggaccccg ggggccccan ggggactgtg ccccaggccc ctccctcgtg 60
gaccgaccgc cttcctcgct tctccttggc ctggagaggt tccttccaat ggccctgagc 120
tgggggagac agacaaatgc gacaaggacg ggaggagcag ctggagggac cgacatttga 180
cttaccaagt agctctttgc gcgccttctt ctgcagcttc agcgtgtccg acgacttctt 240
tttgatctca tgccgggctc gtttgtactc tttcgcgtgg tccttgtcca gctggttggc 300
cgccttcttc cagtcctcga tgcgctcctg cagcgggttg atgaggctct ccagcagtgc 360
gttggtgaac tgccgcagct tggtctcgat get 393
<210> 19
<211> 676
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:407570.5:2000SEP08
<400> 19
agagtcttct ggctgtgctg cctcctctgc ttttaatctt ctgtggatat ttctccgatt 60
tcccccaaaa gcccacagat cctgggggaa gccaccccat tcggccaccg atcatggaag 120
aaagtattac tttttattta ccaaacattt taaagatgtt ggaagatcca tatgctgcct 180
ggaggctgtt gtttctaggg acacaagtat ccagcctata ttgttacaat tttttttcaa 240
gaactgccgt cttctttgcc tgcactaatg gaacatcatc ttcagcgggc tatttcagca 300
cagcaggtgt atggcgagaa gagggataat atggttatac cggtcccaga ggcagaaagt 360
aatattgctt actatgagtc tatatatcct ggggaattta agatgccaaa gcagctcatt 420
cacatacagc gtaagtaatg attctcttaa tgattgtatt ttctatattt ctcttattat 480
gtaacttgac agatttttat ctcttcagga gcgtttttgt tatttgtttc attgaccagc 540
atcagcttaa gagttagtag agtccatttt caacttttaa aatgatattt gagatatctg 600
acaagaaaag atagcattta ttattctaca ttttttgttt cattccagca tacaatctta 660
tgctgcttta gtcaag 676
<210> 20
<211> 988
<212> DNA
11/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
<213> Homo Sapiens
<220>
<221> misc_feature
<2-23> Incyte ID No: LG:337835.8:2000SEP08
<400> 20
cagcagtcag aaaagaggtg caggggcccg ggctgggaca gtgaagagtg ctgggcagtc 60
tgtggtcctc tgtatctcaa ctttttcatc ttaaaaaaac aaatagggtt gtgtgtgtgg 120
ctggtggtca taaggtcctt tctggctcta ataacctgag cttctgttat gaagctggga 180
cccttagagc ctcaggatga tcctctgttt gtttgtgaag ccccaatcag gtgctaagca 240
ccatagtggc acttagctga agctcctctg taactcctgt gggccctgcc ttgcccaccc 300
ccgacagctg ctgcagtgct cctgagcagc acaggcctga tggagcttct ggagaagatg 360
ctggccctca ccttggcaaa ggcagattct cccaggactg cactcctctg ctctgcctgg 420
ctgctcactg cctccttctc tgcccagcag cacaagggca gtttgcagaa ggaccctcta 480
ttgtcccagg cctgtgttgg ctgcctggag gccttgcttg actacctgga tgcccggagc 540
ccagacattg ctctccacgt ggcctcccag ccttggaatc ggttttgctg tttaccctct 600
tggatgctgg agagaattcc ttcctcagac ctgagatttt gaggctcatg accctgctcc 660
agagcatggg acacctggct gaccacagca tggcccagac cctgcaggcc tccttggagg 720
gccttccccc tagcacctcc tcaggccagc cacccctgca ggacatgctc tgcctgggag 780
gggtggctgt atccctgtcc cacatcagaa actgatcctc aggacttgaa ggcccagaag 840
tggagagaga atgagacctg gagacaaagg gcataattgt tggggaaatg gatgacagct 900
gaagctattc atatggagcc atatactcta ttgttgaaat agaataagga aataaaatga 960
tacactcaca taaaaaaaaa aaaaaggg 988
<210> 21
<211> 470
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:1099283.1:2000SEP08
<400> 21
gcccggctgg ggacccgcgc acctgcagcg cccgctgctc ggccctgcat cctgcctggg 60
catcctgcgc ccggccatga cggcgcactc attcgccctc ccggtcatca tcttcaccac 120
gttctggggc ctcgtcggca tcgccgggcc ctggttcgtg ccgaagggac ccaaccgcgg 180
agtgatcatc accatgctgg tcgccaccgc cgtctgctgt tacctcttct ggctcatcgc 240
catcctggcg cagctgaacc ccctgttcgg gccccagctg aagaatgaga ccatctggta 300
cgtgcgcttc ctgtgggagt gacccgccgc ccccgaccca ggttcagctc gaagatattt 360
aaaaaccact tgctccatga acctttccat aagatcccag tcttcaatga ttccatgtcg 420
tatcggccac tggaggaaga gacaccagag ttgtcaagcc tcagttcaca 470
<210> 22
<211> 548
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:401274.2:2000SEP08
<400> 22
caggctgggg catcctcgcc ctggctcttt gagcccggac cagacaggtg ctgggaatac 60
agcaaggaat tagaccagtt ccctgctctc tggtatggtg ggacagatac taaaaagaga 120
12/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
tgtgataatg gatcatcatg tttctaccat caagcctcga agaatccaaa accaaaatgt 180
cattcaccgt ttggaacgcc ggcggatcag ttcaggcaag gcaggtaccc actggcacca 240
agtccgagtg ttccatcaga atgtcttccc caacttcaca gttgtcaacg ttgaaaagcc 300
tccttgtttc ttgcgtaaat tctcacctga tggacgctac tttattgctt tttcttcaga 360
ccagacatct cttgaaatct atgagtacca gggctgccag gcagcagagg acctactgca 420
gggatacgaa ggagaaatcc tgtccaatgg caatgaccag cggtcagtga atatccgggg 480
ccggctcttt gaacgctttt ttgtcctgct gcacattacc aatgttgcgg ccaatggtga 540
gcacctga 548
<210> 23
<211> 2592
<212> DNA
<213> Homo Sapiens
<220>
<221> misc feature
<223> Incyte ID No: LG:222880.1:2000SEP08
<400> 23
cgtcgcccaa ggtcgcgggc cgcttgggga gtcagcagcg cgccaggccc cttcgggccc 60
cacacgcatt aggtgccttc tagaagggta cggagtgaac gcgggcggcg gcgggaccga 120
ggcagcgccc agtttgtaac cgccgcgccg cccgtgcccg cgcgcgccac accccagcgc 180
gcttccggcc gggccacgtg accgcgcgtg cacgtgttcc ggcctctccg cttcgccgct 240
ccgaacctcc tcctggtcgt cccggcattc gtccacgcgg agccggcttg ggcggggccc 300
gggaggcggc ggccggagaa gccgcggaga cgcgagcgcc gagcgtcgcg agggagcagg 360
cccgggcagg caatgcggcg gcctccgcca tgaaccccag gggcctgttc caggacttca 420
accccagtaa gtttctcatc tacacctgcc tgctgctctt ctcggtgctg ctgcccctcc 480
gcctggacgg catcatccaa tggagctact gggccgtott tgcccccata tggctgtgga 540
agcttctagt cgtcgcaggc gcctccgtgg gcgcgggcgt ttgggcccgc aaccctcgct 600
accgcaccga gggagaggcc tgtgtggagt tcaaagccat gctgatcgct gtgggcatcc 660
acctgctgct gctcatgttc gaagtcctgg tctgcgacag ggtggagagg ggcacccact 720
tctggctgct ggtcttcatg cctctcttct tcgtgtcccc cgtgtccgtg gctgcctgcg 780
tctggggctt tcgacacgat aggtcgctgg agctggagat cctgtgctcg gtcaacatcc 840
tgcagttcat cttcatcgcc ctaaagctgg acaggattat tcactggccg tggctggtgg 900
tgtttgtgcc cctgtggatc ctcatgtcgt tcctttgcct ggtcgtcctc tattacatcg 960
tctggtccct cctgttcctg cggtccctgg atgtggttgc cgagcagcgg agaacacacg 1020
tgaccatggc tatcagttgg ataacgattg tcgtgcctct gctcactttt gaggtcctgc 1080
tggttcacag attggatggc cacaatacat tctcctacgt ctccatattt gtcccccttt 1140
ggctttcctt actaacttta atggccacaa catttaggcg aaaggggggc aatcattggt 1200
ggtttggcat tcgcagagac ttctgtcagt ttctgcttga aattttccca tttttaagag 1260
aatatgggaa catttcatat gatctccatc acgaagatag tgaagatgct gaagaaacat 1320
cagttccaga agctccgaaa attgctccaa tatttggaaa gaaggccaga gtagttataa 1380
cccagagccc tgggaaatac gttccccccc ctcccaagtt aaatattgat atgccagatt 1440
aaactcctag agaggaccca ggcacacaca gactccactt ggccttcgcc tcttgttcat 1500
tcatcccaaa cctggaaatg gaaacaggct tcaaacactc gtctcacgcc gtgtttgaga 1560
tcaccgcctc atcagtatgc atcatagatg gaggtggttt cagtatgtgg gtgtgtgtgg 1620
tgtgtacctg ggtaagagac ttgctttcca ggttcgcact ttcaggtgta gctgggggca 1680
gtaagtcgaa ttgttttagt aggtcctcaa aaggaataac cacacagctg tttgtttaaa 1740
tgctactgta cctatcaaaa ctattgttta aaaagtattt ttatacactg ctaatctaaa 1800
attgtatttc agattgtgcc tgtcataaca atagcaaatg taaaaagttc tctttcccac 1860
cacttgttta taaacctcat agttgatatt tttagtgttc ctactgttaa aatactctct 1920
ccttgggctt tgctgatact ggtctttaat attctgatag gtgaattttt ctaatggaat 1980
gaacccatgc atatatagta tttatatgaa tattttagca gtgtaatatg ttgaattcta 2040
gttctctgca ttaccattat tacgttaaag tattttttaa agcttaggtg tgaagatatg 2100
tgtctattgc agatgtcctt gaaaactgca taaaacagta tgtgcctggt gtggatctta 2160
ccaaagtact aggcatgaat gtagggactg caaatcccat gggtcttaat atttaggtgt 2220
13J236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
tagtaaccaa ggtctctggt agtacccgtt agtagaggaa gaggccactg cccttgggaa 2280
cttgtgacag gctctagtgt ggtaccaggc cataaagtga cactgttatt tagcaacttg 2340
aatttttcca cacaggtagt aactgtgtgg aaataagcaa caagtggttt gtccatttct 2400
aagaatctta aactattagt tggctgtagt gtgaagcatt acttgtcatt ggaaagatgg 2460
agagagtggc cttaaccgga agtggtcagt agaagcaggt gtcattttaa gggccaaact 2520
ttaatctgtc agcaataggg aaacaactgt tcaaattatc tttgtagata agaacagtgt 2580
ttcttttttc tt 2592
<210> 24
<211> 1661
<212> DNA
<213> Homo sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:406389.1:20005EP08
<220>
<221> unsure
<222> 677
<223> a, t, c, g, or other
<400> 24
gacgcggggc gccagctgcc aactgtgcgc gcgcggagct ccccggcggt gcagtcccgt 60
cccggcggcg cgggcggcat gaagactagc cgccgcggcc gagcgctcct ggccgtggcc 120
ctgaacctgc tggcgctgct gttcgccacc accgctttcc tcaccacgca ctggtgccag 180
ggcacgcagc gggtccccaa gccgggctgc ggccagggcg ggcgcgccaa ctgccccaac 240
tcgggcgcca acgccacggc caacggcacc gccgcccccg ccgccgccgc cgtcgccgac 300
aacgcctcgg ggaacggccc ccctggcggc gcgctctaca gctgggagac cggcgacgac 360
cgcttcctct tcaggaattt ccacaccggc atctggtact cgtgcgagga ggagctcagc 420
gggcttggtg aaaaatgtcg cagcttcatt gacctggccc cggcgtcgga gaaaggggtc 480
ctgtggctgt ctgtggtctc cgaggtgctg tacattctgc tgctggtggt tggcttcagc 540
ctcatgtgtc tcgagctctt ccactccagc aatgtcatcg acgggctcaa gctcaatgcc 600 w
ttcgcggctg tcttcacggt gctctcaggc ctcctgggaa tggtcgccca catgatgtac 660
acgcaggtgt tccaggncac cgtgagcctc ggtcctgagg actggagacc ccattcctgg 720
gactacgggt ggtccttctg cctggcgtgg ggctccttta cctgctgcat ggcagcctct 780
gtcaccacgc tcaactccta caccaagacg gtcattgagt tccggcacaa gcgcaaggtc 840
tttgagcagg gctaccggga agagccgacc ttcatagacc ctgaggccat caagtacttc 900
cgggagagga tggagaagag ggacgggagc gaggaggact ttcacttaga ctgccgccac 960
gagagatacc ctgcccgaca ccagccacac atggcggatt cctggccccg gagctccgca 1020
caggaagcac cagagctgaa ccgacagtgc tgggtcttgg ggcactgggt gtgaccaaga 1080
cctcaacctg gcccgcggac ctcaggccat cgctggcacc agcccctgct gcaagaccac 1140
cagagtggtg cccccagaac cctggcctgt gtgccgtgaa ctcagtcagc ctgcgtggga 1200
gatgccaggc ctgtcctgcc catcgctgcc tgggtcccat ggccttggaa atggggccag 1260
ggcaggccca agggaatgca cagggctgca cagagtgact ttgggacagc agccccggac 1320
tcttgccatc atcacatgag ccctgctggg cacagctgcg atgccaggag acacatggcc 1380
actggccact gaatggctgg cacccacaag ccagtcaggt gcccagaggg gcagagccct 1440
ttggggggca gagagtggct tcctgaagga gggggcagtg gcgcaggcac tgcaggggtg 1500
tcacacagca ggcacacagc aggggctcaa taaatgcttg ttgaacttgt tttcttgcgg 1560
tgtatggagg ccagttgctc tgtgggctgt ggctggcctg tcattgaccc aacccattac 1620
tcctcaagga ggctgaaggt gacagcagag actgtgcaga g 1661
<210> 25
<211> 1613
<212> DNA
<213> Homo Sapiens
14/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
<220>
<221> misc_feature
<223> Incyte ID No: LG:055461.1:2000SEP08
<220>
<221> unsure
<222> 1554
<223> a, t, c, g, or other
<400> 25
tcaacttgaa atgccaagca ttaaatgagc cacaaagatt gaattcccat tatccttgaa 60
tcagagcaag cctgtcgtgc agccatgagt tagtggagaa aaatccggag ttagagaaga 120
ttataaatgg cagagccatt taactgtggt tagctgttgg attctgatac tttcttaaaa 180
atacgttctt gcaccaacat cttcattggg aacagttcag aaaaagcaaa gaggagagcc 240
aacattcaca tttaccatgg ctataccaat aacagtgctt gactgtgacc tcttgctata 300
tggccgtggt caccggacat tggaccgttt taagctggat gatgtgactg atgaatactt 360
gatgtccatg tatgggtttc cacggcagtt catttattac ttggtggagc tcttgggggc 420
gaatctttct aggcctactc agcgatccag ggctattagc ccagagacac aggtccttgc 480
agcattgggt ttttatacct caggttcctt ccagactcgg atgggagatg ccattggaat 540
cagtcaggcg tctatgagtc gttgtgttgc caatgtcact gaagcacttg tggaaagggc 600
ctcacagttc attcgctttc cagctgatga agcctccatt caggctctga aggatgaatt 660
ctatgggttg gcagggatgc catgggtgat gggggtggtt gactgtatcc atgtggccat 720
caaggcacca aatgctgaag acctctccta tgtgaaccga aaaggcctgc attctttaaa 780
ctgcctgatg gtgtgtgaca ttagagggac actaatgacc gtggagacaa actggcccgg 840
cagcctacag gactgtgctg tgctgcagca gtcttccctc agtagtcagt ttgaagcggg 900
tatgcacaaa gatagctggc ttctgggtga cagttccttc tttcttcgaa cctggctcat 960
gaccccactt cacattcctg aaactccagc agaatatcgc tataacatgg cccattctgc 1020
aactcacagt gtgattgaga agactttccg aaccctctgc tcccgattcc gctgcctgga 1080
tggatccaag ggggcactgc agtactcacc agagaaatcc agccatatca tcttggcctg 1140
ttgtgtcctc cacaaacatc tccctggagc atgggatgga tgtttggtcc tctccaatga 1200
caggacccat ggaacagccc ccggaagagg agtatgagca catggagtcc ctggacttag 1260
aggctgaccg tattcgtcag gagctaatgc tcactcattt tagctaatgt agaaggtgga 1320
gaggagggat acttcccagg agttgtgaca gactttcctc ctcatcacct tttacacagt 1380
tccatcatct agcatgactg agtatacaga tacttgtcat aaactgacat ttaatatgtg 1440
tgttttggta aggttggggc tatgccagaa tatcttgatt catttgcata tgcattaatt 1500
aaactgaaac caagacagcg gctccctact atccagtgaa ctctaggttg agtnccacta 1560
atttgaaagc tcagtggttg caaacattta tgactgtgcc tacaaagtca tag 1613
<210> 26
<211> 658
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:979059.5:2000SEP08
<400> 26
gctactgaca tgcagaggaa gagaagcagc gaatgccttg atggcacatt gactccttct 60
gatggacaga gtatggagag agctgagagc cccacaccag gaatggccca gggaatggag 120
ccagatggct acaaggtagt attcgtgcgc cggagcccgc tggtgctagt ggcggtggct 180
cgtacgcggc agtcggcaca agagctggcg caggagctgc tctacatcta ctaccagatc 240
ctaagccttc ttaccggtgc gcagctgagc cacatcttcc agcagaagca gaactatgat 300
ttgcggcgcc tactctcggg ctcagagcgc atcaccgaca acctgctgca gctcatggca 360
cgagacccca gcttcctgat gggggcggca cggtgcctgc ccctggcggc ggccgtgcgc 420
gacactgtga gcgccagcct gcagcaggcg cgtgcgcgca gcctggtctt ctccatcctg 480
15/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
ctggcccgca accagctcgt ggcactcgtg cgcgcagcct ggtcttctcc atcctgctgg 540
cccgcaacag ctcgtggcac tcgtgcgccg aaaggacaat ttctgcaccc atcgactgac 600
ctgtcttcac tcattagttc tcctcgtctt tcgcgaggcg agctggagcc gtgtgctg 658
<210> 27
<211> 1950
<212> DNA
<213> Homo Sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:399238.1:2000SEP08
<400> 27
ccgggcgctc gggcaggtat tgtccgtccc tccgggcttt gtagaatcgt cgccggctta 60
cctggccgtg ggcgcgtcct ggccgctgca gcccggagca gggtgcggcg gcggcggcgg 120
cggctgatgg tgtccccggt cactgtggtg aagagtgaag gacccaaact ggtgccgttc 180
ttcaaggcca cctgcgtgta ttttgtgctc tggctgccct catctagccc atcgtgggtc 240
agcaccctca tcaagtgcct gcctatcttc tgcctctggc tcttccttct ggcccatggc 300
ctgggattcc tgctggccca ccccagcgcc acccgcatct ttgtggggct tgtcttctct 360
gctgtaggtg acgccttcct catctggcag gaccaaggat acttcgtgca tggtctgctg 420
atgtttgctg tgacccacat gttctacgcc tcggcctttg gcatgcagcc actggctctt 480
cggacaggtc tggtgatggc agcgctgtcg ggcctgtgct atgccctcct ctacccatgc 540
ctctcaggtg ccttcaccta cctggtgggg gtctatgtgg cccttatcgg cttcatgggc 600
tggcgagcta tggcagggct gcggctggcc ggggcagact ggcgctggac agagctggca 660
gctggcagtg gtgcactctt ctttatcatc tcagacctga ccatcgccct caacaaattc 720
tgttttcctg tgccctactc tcgggcgctt atcatgtcca cctactatgt ggcccagatg 780
ctcgtcgcct tgtcagctgt cgaaagccgg gagcctgtgg aacactacag actgaccaag 840
gccaactgag gtgccagggt ctggtcaccc ctctctcctc ctggggctgg ggcccagatc 900
ctggggacct gcaggagctg gatcaggatg gctgcagtgc cagcctgggg cagcaggtac 960
tgcctgagga atttgcaagt tcgtgtgggg aggctggaaa aggatctccc tagcagaact 1020
cgtggttcag gacaatgctg agagctaaaa gagccagcct aatatcagac tggagccaga 1080
agtagacatc tccccacctc taccctatca ggactttcaa aacccccctg gaaggtgatg 2140
gtgctcagct tcttgacacc cccccaccca cttcccctac caggtggaag agtctgactc 1200
catgtgttat gcctaggacc aatggcagtg ctgggttcac ccactcctcc cctttttcat 1260
ctgcacaaag ttgagggaaa cgggagaatc tgtgctgaga agactcagac ccaggagccc 1320
ctttcacagg cccctagttg ggaagactgg atgagagtgg agcctccgtt tcttttccct 1380
cttctatcct tgttacttga acaatctcag tctctaagtg gtgggtgggg acatgaagag 1440
gtgtgggtcc aggttaggca ggagagagaa cttctttggt ctcggagttt ggggtctcaa 1500
gaattcaagt agccccatct ctctctcggg caagcccaaa gctcaggtcc catacgctac 1560
ccttagatcc tatccccagg gctagggtga accatgccaa aggaaggggc cagcttctct 1620
gtgtgtgtgt gtagcctgtc tcctagtctg cctatgtctt tctctgctat atgtttctgt 1680
cctgctgtct gtcaaaagtc tcacagagag agggcctcag ggagctgctg aggggtgctg 1740
agcctggctc agggagctgg gacccctccc ctccaacaca catactcaca gtgctttcct 1800
ccttgtccct cctacactag gagcaagagg agggggctcc aatgacactc tggggtttta 1860
agacccaagg cacattcaat gcaaggggag caaggatggg acaactccat cttctgctgc 1920
ccatgtgaaa aataataaaa atcaagagcc 1950
<210> 28
<211> 572
<212> DNA
<213> Homo sapiens
<220>
<221> misc_feature
<223> Incyte ID No: LG:1382945.7:2000SEP08
16/236
CA 02419943 2003-02-20
WO 02/20756 PCT/USO1/27297
<400> 28
tatttaattg gaaattttaa ttcgctccca gtgaatcaat agagtgtcat ttaggaggat 60
ttcctaatgc tggctcttta attctagaga tattatatgc ctggtaagtc aagggttgat 120
tttcagaagc tccattattt tataagaatt gtgtgttttt tatttgaaac atagtatata 180
gatcgtttaa atgaaatttc acaaaactat tctttttttc ccatcactca gggttatgtg 240
cttcacatga tcactactgc agcagaatgg tctatgtcat tttccttctt tggttttttc 300
ctgacttaca ttcgtgattt tcagaaaatt tctttacggg tggaagccaa tttacatgga 360
ttaaccctct atgacactgc accttgccct attaacaatg aacgaacacg gctactttcc 420
agagatattt gatgaaagga taaaatattt ctgtaatgat tatgattctc agggattggg 480
gaaaggttca cagaagttgc ttattcattc atcatgaaat atttcaacca cttaatcaag 540
gctgacagta acacatgatg aatgctgata at 572
<210> 29
<211> 2638
<212> DNA
<213> Homo sapiens
<220>
<221> misc feature
<223> Incyte ID No: LG:1383610.3:2000SEP08
<220>
<221> unsure
<222> 1998
<223> a, t, c, g, or other
<400> 29
ataattatgg cggccacagt tcattggccc tttcaagtag aaggaatagt ttttgaccat 60
aaaaagtcaa actagaaagc cattaatgtc caacttccag agtagatttt ctgccttgga 120
gcagaagagg tgtgtgccga gttttatgtc tggcaaataa tgttagaggc caaacttggg 180 , .
ccctgcagct ccctcactcc acctggcatt tgccaggtac tgctctgggc tctggggaca 240
ccgcggtaaa caatatcctg gccctcatgg gagccatctt ctactcaggg gaagcattgg 300
ataaacaggt ttcgttgagt gccctaatca gggtactgtg ttagagtagg agggaggaag 360
gcagaaggaa ggatgccacc ttgaatagca tggtcacaga aggcctgtct gaagaggagc 420
aatttgagtg ggaccttgaa ggtgagaagg agccaagggg gcagagctgg gaggacattc 480
caggcagagg gaatcccaag tgcagtcctt aaggtgagga agccagcagg aggtggcgtg 540
tctctagatg aggtcaggga ggtgggcggg caggactcag gcacagaagg ctctgaatag 600
gccatggtgc tctttatatt aatctcgaga acagtgggaa ggagggatct ttaaatgggg 660
agcaacttgt ttggtttgtg tttttaaagc tctctggaga aggaattaca ggcagaaaag 720
ggtagggcca gtggctggag gggagacccc ccaagattta gaggccagca aggaggagag 780
gacaggccaa tagctaggag gaaatccagg agaggatgtg ttaccaaaac gaaaagagga 840
gcttcttcag gaggaggaag tggaccctct gctcagggca acggaactat caaggagggt 900
gagaagagac gtgtccctcc atccttagtt aaatgagtct ggggtgccaa gaattttcat 960
gcatgtttat taatctcaat aacaatcctg tttttttgcc ctcaaaatgt gttcaagata 1020
tttgtagatg tcatcttctt aatccttgta gtatcctgtt tgagtaagtg tcatcttgga 1080
agttaagtaa gttgtcaagg gtcagctcac taggaagtgg tgataccaga atatgaacca 1140
gtttcccgaa gcccagagcc tgaagccatg cgaaagccag cagggtgact cctccggctg 1200
caggcgagtg ggctgtgttt cacccggttt ctccttttct aggttacggc ctccgctgcc 1260
acagggccat catcaccatc tgccggctca ttggcatcaa agacatgtat gccaaggtct 1320
ctgggtccat taatatgctc agcctcaccc agggcctctt ccgtgggctc tccagacagg 1380
aaacccatca acagctggga ggacattcca ggcagaggga atcccaagtg cagtccttaa 1440
ggtgaggaag ccagcaggag gtggcgtgtc tctggccttg tgcagccagt tcctgtgctg 1500
ccctgcacct aggagagact cagcccctca cagcttggga tgttaccttg ccttttgttt 1560
gttttgaggg aagtttaatc tttaaactct ttggaaataa ataattatag ctttcatttg 1620
ttgagcacat gttatatgcc aatgtgatag aacctttaca tacatatctc agttcaagac 1680
tactttaaat attcatccaa agtaacaaaa gtaaatgaat tagggagacg gggttaataa 2740
17/236
DEMANDE OU BREVET VOLUMINEUX
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