Note: Descriptions are shown in the official language in which they were submitted.
. -
1
METHOD AND MEANS FOR ACCLIMATIZING SEEDLINGS FOR OUTDOOR
LIFE
This is a division of Canadian patent application serial no. 2,815,886 filed
December 9,
2011.
TECHNICAL FIELD OF INVENTION
The invention relates to a method and device for acclimatizing seedlings for
outdoor life.
More particularly the invention relates to lighting method and devices that
can be used to
treat a seedling in a growth chamber or greenhouse prior to the introduction
of the seedling
to outdoors.
BACKGROUND
Industrially produced tree seedlings are typically grown in growth chambers or
greenhouses at early stages of their life. When the seedlings reach a certain
age or size,
they are then subsequently planted outdoors in accordance with the prior art.
The move
out-doors is a very shocking experience to the plants. Among other things, the
"transplantation shock" to the plant from moving outdoors comes from the
difference in
the light spectrum inside (the greenhouse or laboratory growth chamber
conditions) and
outside. In order to avoid this plants are often kept under shading curtains
for a few weeks
in order to limit the direct exposure to sunlight. This period causes
additional work and
investment to the grower and delays the maturing of the harvest.
The shock to the plants is caused by the abrupt exposure to the Sun, as the
light they have
received in the greenhouse or growth chamber has had a limited light spectrum
compared
to the outdoors and the light spectrum of the Sun. The light conditions
indoors is
comprised of Sun light, typically filtered through the greenhouse glass or
polycarbonate,
and in most cases also additional artificial light from high-pressure sodium
lamps during
hours with less natural light. Fluorescent tubes are often used in growth
chambers. Special
LED based lights are also getting more common, which may/will replace UPS
lights and
fluorescent lights in the future.
CA 3055322 2019-09-12
2
Tree seedlings have thus been reported to suffer from a 'transplant shock',
i.e. seedling
mortality or impaired growth, after they have been introduced outdoors.
US 2008/0120736 describes a method of illumination of plants in the PAR
(Photosynthetically active radiation) and UV-A and UV-B, or infrared regions
of the
spectrum. The UV illumination is alleged to increase insect resistance, immune
response,
enhance pigmentation and aroma, and alter plant architecture such as shape,
flower number
and volume and trichome density. This document is also cited here as
reference.
It is further known that UV radiation stimulates the production of phenolic
compounds
protective of excess radiation. Also it is known that many plants in high
light environments
increase the reflectance of their leaves by acquiring a coat of leaf hairs or
wax, as a means
of external photoprotection. This document is also cited here as reference.
These two
phenomena are well known among professional plant photobiologists, but they
have not
been utilized in any practical way.
EP 0364952 A2 shows a method of irradiating seeds with UV. The viability of
seeds is
tested with this method, as non-viable seeds cause fluorescence of sinapine.
In summary, it appears that in the prior art UV enhancement of plants is known
to provide
a variety of photomorphogenic and other effects. In addition UV illumination
is used as a
method to detect the viability of seeds.
The methods of the prior art have considerable shortcomings. The detection of
seed
viability is essentially useless if the seedling eventually dies of the
aforementioned
transplantation shock. Furthermore, photomorphogenical enhancement of plants
by
increasing their size or flower number is also ineffective in view of the
eventual
CA 3055322 2019-09-12
3
outcome, if the seedling does not survive the transplantation shock. Using
shading by
curtains is uneconomical as it disproportionately increases the sorting of the
seedlings
done by the plant producer. This is because transporting the plants under the
shades
and removing them from under the shades also adds one additional costly work
phase
to the seedling producer.
SUMMARY
The invention under study is directed towards a system and a method for
effectively
treating tree seedlings against the transplantation shock prior to their entry
to
outdoors.
First objective of the invention is to present this treatment to the seedling
in a manner
that takes place in a short period of time, thereby minimising the sorting of
seedlings
that the plant grower needs to manage. Further objective is to provide a
treatment for
transplantation shock that is effective on northern and southern latitudes, or
any
different latitudes, as the amount of sunlight, and therefore the requirements
for
successful transplantation shock treatment might vary with latitude.
Another objective of the invention is to provide a transplantation shock
treatment that
can be applied to seedlings grown in a dark growth chamber, or in shadow due
to e.g.
stacking, or in any growth environment characterized by the absence of natural
sunlight.
Third objective of the invention is to provide a transplantation shock
treatment that
can be applied to plants housed in a greenhouse wherein the seedlings receive
some
natural sunlight and artificial light is used to supplement this natural light
for
transplantation shock treatment. An even further objective of the invention is
to
provide artificial light that can be applied during the night in a greenhouse
to
seedlings and/or plants for transplantation shock treatment.
CA 3055322 2019-09-12
4
- One aspect of the invention is a light by which the plants can be
prepared for the
outdoor conditions, by giving them certain wavelengths of light they do not
currently
receive from the light in the greenhouse or growth chambers. The light of the
invention can be applied in smaller doses during the major part of nursing of
the
seedlings or as a "sun-shock" period in the end of the indoors nursing period.
By
giving the seedlings light from the invention, they are prepared to Sun light
and do not
need to spend a few weeks under the sunshade curtains.
In one embodiment of the invention, the growth chamber lighting device emits
radiation that gives the plants the parts of radiation they have not received
during their
growth period. The key spectral areas of the device are the UV-A (315-400 nm),
UV-
B (280-315 nm) and as well as the violet and blue areas (400-500 nm), as well
as red
and far red areas (600-800 nm). In some embodiments of the invention, the
device
may also contain green and yellow areas of the spectrum (500-600 nm).
The invention thus improves the growth cycle of tree seedlings, enhances the
proportion of viable seedlings and eliminates one work phase in the growth
process,
thus improving the economics of seedling cultivation and growth.
A method of treating plants is in accordance with the invention and
characterized in
that,
-at least one said plant seedling is housed indoors,
-the said at least one plant seedling is exposed to artificial UV light
indoors prior to
outdoor life,
-at least a part of the incident UV light is produced by light emitting diodes
(LEDs).
A method of treating plants is in accordance with the invention and
characterized in
that,
-at least one said plant seedling is housed indoors in a transparent
greenhouse,
-the said at least one plant seedling is exposed to artificial light indoors
prior to
outdoor life,
-at least a part of the artificial light is produced by light emitting diodes
(LEDs),
CA 3055322 2019-09-12
. -
. 5
-the spectrum of said artificial light when combined with sunlight spectrum
transmitted through the said greenhouse is arranged as similar to sunlight
outdoors on
Earth.
A method of treating plants is in accordance with the invention and
characterized in
that,
-at least one said plant is a tree seedling and is housed indoors,
-the said at least one tree seedling is exposed to artificial UV light indoors
prior to
outdoor life,
-at least a part of the incident UV light is produced by light emitting diodes
(LEDs).
A light device for plant treatment is in accordance with the invention and
characterized in that, at least one said light device is a UV LED and is
arranged to
illuminate at least one plant seedling indoors prior to the transfer of said
at least one
plant seedling to outdoor life.
A light device for plant treatment is in accordance with the invention and
characterized in that, at least one said light device is a UV LED and is
arranged to
illuminate at least one tree seedling indoors prior to the transfer of said at
least one
tree seedling to outdoor life.
Use of artificial light to treat transplantation shock in plants is in
accordance with the
invention.
According to one aspect of the invention, the tree seedlings are grown in a
greenhouse
with transparent or semi-transparent walls and ceiling, typically made of
polycarbonate or any other plastic and/or glass. In this embodiment artificial
light is
shone on the seedlings from LEDs that supplement the natural spectrum that
transmits
through the walls and ceilings of the greenhouse. As we know that high energy
photons get cut off by some materials in greenhouse walls or ceilings, the
artificial
light typically emits UV photons. The compound spectrum of the natural light
and the
artificial light is arranged to treat the seedling against transplantation
shock. In further
CA 3055322 2019-09-12
6
aspects of the invention the artificial light is used during the night or when
the Sun is low
on the horizon to treat the transplantation shock.
According to another aspect of the invention, the tree seedlings are housed in
a dark
growth chamber where there is no natural sunlight. In embodiments where the
sole source
of light for the plant is artificial light, the transplantation shock
treatment can be arranged
to be conducted by the primary light source at some stage in the cultivation
period of the
seedling, or by a special light source that will be used at different times.
The special light
source providing the transplantation shock treatment may be integrated in the
primary
growth light device. The spectrum of the light for transplantation shock
treatment needs to
form a preferred compound spectrum with primary light source when it is in
use, and/or
form the entire transplantation shock treatment spectrum when no other lights
are in use.
According to yet another aspect of the invention, there is provided a method
of treating
plants, wherein at least one of said plants is at least one tree seedling, and
wherein the
method comprises: before planting the at least one tree seedling outdoors,
cultivating the
tree seedlings indoors while also exposing the at least one tree seedling to
daily, non-
continuous doses of high-intensity artificial UV light, in order to
acclimatize the at least
one tree seedling to outdoor life, wherein at least a part of the UV light is
produced by light
emitting diodes (LEDs).
According to still another aspect of the invention, there is provided a
lighting arrangement
light device for plant treatment, the lighting arrangement comprising: a
housing
comprising at least one tree seedling within the housing, at least one said
light device
wherein the light device comprises a UV LED and is configured to illuminate
the at least
one tree seedling with daily, non-continuous doses of high-intensity
artificial UV light
prior to transfer of the at least one tree seedling to outdoor life in order
to acclimatize the
at least one tree seedling to the outdoor life.
CA 3055322 2019-09-12
.=
6a
According to one aspect of the invention, there is provided a method of
treating plants,
wherein at least one of said plants is at least one plant seedling wherein the
plant is not a
tree, and wherein the method comprises:
before planting said at least one plant seedling outdoors, cultivating the
plant seedlings
indoors while also exposing said at least one plant seedling to daily, non-
continuous doses
of high-intensity artificial UV light, in order to acclimatize said at least
one plant seedling
to outdoor life, wherein at least a part of the UV light is produced by light
emitting diodes
(LEDs).
According to another aspect of the invention, there is provided a lighting
arrangement for
plant treatment, the lighting arrangement comprising:
a housing comprising at least one plant seedling wherein the plant is not a
tree within the
housing,
at least one light device wherein the at least one light device comprises a UV
LED and is
configured to illuminate said at least one plant seedling with daily, non-
continuous doses
of high-intensity artificial UV light prior to transfer of said at least one
plant seedling to
outdoor life in order to acclimatize said at least one plant seedling to the
outdoor life.
Some or all of the aforementioned advantages of the invention are accrued when
the
transplantation shock treatment is adjusted so that it interferes with the
growth of the plant
as little as possible.
In addition and with reference to the aforementioned advantage accruing
embodiments, the
best mode of the invention is considered to be the provision of small doses of
high energy
UV pulses to tree seedlings housed in a chamber with very limited access to
sunlight.
BRIEF DESCRIPTION OF THE DRAWINGS
In the following the invention will be described in greater detail with
reference to
exemplary embodiments in accordance with the accompanying drawings, in which
CA 3055322 2019-09-12
6b
Figure 1 demonstrates an embodiment of the inventive method of treating plants
for
transplantation shock as a flow diagram.
CA 3055322 2019-09-12
7
Figure 2 demonstrates an embodiment 20 of the transplantation shock treatment
of the
invention when used in a greenhouse as a block diagram.
Figure 3 demonstrates an embodiment 30 of the transplantation shock treatment
of the
invention when used in a growth chamber as a block diagram.
Figure 4 demonstrates an embodiment 40 of the inventive method of treating
plants in
a greenhouse for transplantation shock as a flow diagram.
Figure 5 demonstrates an embodiment 50 of the inventive method of treating
plants in
a growth chamber with limited or no sunlight for transplantation shock as a
flow
diagram.
Figure 6 demonstrates an embodiment 60 of preferable LED spectra used in
accordance with the invention that have been built and tested by the
applicant.
Some of the embodiments are described in the dependent claims.
DETAILED DESCRIPTION OF EMBODIMENTS
Figure 1 shows a method of treating plants against transplantation shock as a
flow
diagram 10. Typically the said plants are tree seedlings and are housed
indoors at an
early stage of their life. For a tree seedling the planting event is
especially important
as any injury in this phase might mean years of belated or inhibited growth,
or
outright early death to the tree seedling, amounting to substantial economic
loss to the
grower. The method of the invention can in principle be applied with all tree
seedlings, but is especially suited for treating any of the following species
against
transplantation shock: Oak, acacia, pine, birch, maple, sequoia, redwood,
eucalyptus,
bamboo, palm, spruce, aspen, alder, linden, cypress, and/or any other tree
species that
is cultivated indoors in phase 100. In phase 110 the said tree seedlings are
exposed to
artificial UV light indoors prior to outdoor life. At least a part of the
incident UV light
CA 3055322 2019-09-12
8
is produced by light emitting diodes (LEDs) in phase 110. In one embodiment
the said
artificial light is applied in small doses during the major part of nursing of
the
seedlings. In other embodiments a single period and/or a pulse in the end of
the in-
doors nursing period is used.
In some embodiments the said artificial light is applied in any of the
following bands:
UV-A (315-400 nm), UV-B (280-315 nm), violet and blue areas (400-500 nm), red
and far red areas (600-800 nm) andJor green and yellow areas of the spectrum
(500-
600 nm). In some embodiments of the invention the light device is any of the
following: a Light Emitting Diode (LED), AlInGaP red and AlInGaN green and/or
blue HB-LEDs, a light wavelength up-conversion phosphorescing material which
is
deposited in direct proximity of the LED chip, europium-cerium co-doped
BaxSryZnS3
phosphorescing materials and/or cerium doped lanthanide oxide sulfides in
direct
proximity of the LED chip, and/or a wavelength up-conversion device by means
of at
least one semiconductor quantum dot, which is placed near the LED.
In this application "phosphor" is construed to refer to any phosphorescing
material,
which can be for example element phosphor, but it is not limited to only the
element
phosphor. Subscripts x and y denote numerical variables in a chemical formula
in this
application.
It should also further be noted that the embodiment 10 can be readily permuted
and/or
combined with any of the embodiments 20, 30, 40, 50 and/or 60 and be used to
create
any of the embodiments 20, 30, 40, 50 and/or 60.
Figure 2 shows an embodiment where the inventive treatment is administered in
a
greenhouse 200. The greenhouse 200 has typically transparent walls, which are
in
some embodiments made of glass or plastic or a like transparent material.
These
materials typically block the high energy UV with wavelengths of 300- 400 nm
or less
from entering into the greenhouse, resulting to a modification in the spectrum
from
sunlight 230 to filtered sunlight 240. At least one tree seedling 210 is grown
in the
greenhouse in accordance with the invention. The artificial light 220 is
typically
CA 3055322 2019-09-12
9
physically attached to a location from which it has the maximum exposure and
coverage of tree seedlings 210.
In some embodiments the said artificial light spectrum 250 combined with the
spectrum of Sun light transparent through greenhouse walls or ceilings 230
amounts
to a combined sum spectrum similar to the solar spectrum observed on the
ground of
the Earth. This acclimatizes the seedlings to outdoor life in preferable
embodiments of
the invention.
In some embodiments of the invention the green and yellow photons 500-600 rim
are
omitted from the artificial light 220 and its spectrum 250. In some
embodiments of the
invention the greenhouse walls and ceilings might comprise a filter for 500-
600 nin
light, because this band is not as photosynthetically active as blue or red
band as
plants reflect green light, and as this band might create unwanted heat. Green
light is
important to plants in other purposes, for example the plants derive a lot of
photomorphogenetic information from green light, and its spectral ratios with
other
bands. Therefore in a preferred embodiment of the invention, there is green
light
present in the spectrum administered to treat transplantation shock, but this
band of
the spectrum has a smaller relative intensity to blue and red bands than in
the
spectrum of sunlight. In some embodiments of the invention even when the
objective
is to otherwise create a spectrum similar to the Sun indoors, the relative
intensity of
the 500-600 nm band is deliberately left smaller than in the sunlight
spectrum.
The light device 220 is any of the following: a Light Emitting Diode (LED),
AlInGaP
red and AlInGaN green and/or blue HB-LEDs, a light wavelength up-conversion
phosphorescing material which is deposited in direct proximity of the LED
chip,
europium-cerium co-doped BaxSryZnS3 phosphorescing materials and/or cerium
doped lanthanide oxide sulfides which is deposited in direct proximity of the
LED
chip, and/or a wavelength up-conversion device by means of at least one
semiconductor quantum dot, which is placed near the LED. Suffixes x and y
denote
variables in the chemical formula of the compound. Furthermore in some
CA 3055322 2019-09-12
= 10
embodiments of the invention the light device 220 may be equipped with any of
the
following phosphorescing materials expressed with the following chemical
formula:
- MAlSiN3X (where in M is a Metal such as Ca, Sr, Ba and X is
rare earth
element such as Eu in any various ratios and combinations, or X is Mn in any
various ratios and combinations),
- MMgSiOX (where in M is a Metal such as Ca, Sr, Ba and X is
rare earth element such
as Eu in any various ratios and combinations, or X is Mn in any various ratios
and
combinations).
In some embodiments the light device 220 is arranged to transmit a different
spectrum
at night than during the day. In some embodiments the spectrum is arranged to
be
changed dynamically with the time of day or season (i.e. date) or both in
accordance
with the invention.
It should also further be noted that the embodiment 20 can be readily permuted
and/or
combined with any of the embodiments 10, 30, 40, 50 and/or 60 and be used to
create
any of the embodiments 10, 30, 40, 50 and/or 60.
In figure 3 the tree seedlings are housed in at least one growth chamber 360.
The
growth chambers 360 are typically inside a building 300, and the growth
chambers
360 are stacked to save space and cost for the grower. In some embodiments the
growth chambers are transparent and the building is a transparent greenhouse
as
explained before, in some embodiments the building 300 is opaque, in some
embodiments the growth chamber sides are made of opaque material in accordance
with the invention.
In some embodiments where the growth chamber sides 360 as well as the building
300 are of transparent material there is only one or few light sources 320 for
several
tree seedlings. In other embodiments where all or some sides of the growth
chamber
are opaque light sources 321 may be placed closer to, or within the growth
chambers
360 themselves to ensure sufficient treatment against transplantation shock.
CA 3055322 2019-09-12
11
It should also further be noted that the embodiment 30 can be readily permuted
and/or
combined with any of the embodiments 10, 20, 40 and/or 50 and be used to
create any
of the embodiments 10, 20, 40, 50 and/or 60.
.. Figure 4 shows the treatment method embodiment used in the setup of figure
2 as a
flow diagram. In phase 400 the spectrum emerging through the greenhouse walls
and/or ceiling is recorded, for example with a spectrometer. This spectrum is
supplemented by artificial light in phase 410. In many embodiments of the
invention
the artificial light of phase 410 is primarily UV light. This is because the
high energy
.. component typically in the UV has been reflected by the walls and/or
ceilings of the
greenhouse in some embodiments. In phase 420 the compound spectrum is shone on
the tree seedlings.
In some embodiments the artificial lighting supplements the spectrum
differently,
depending what time it is and how much sunlight is available. For example
during the
night the artificial light can be used to produce the whole spectrum, which in
some
embodiments resembles sunlight spectrum.
It should also further be noted that the embodiment 40 can be readily permuted
and/or
combined with any of the embodiments 10, 20, 30, 50 and/or 60 and be used to
create
any of the embodiments 10, 20, 30, 50 and/or 60.
Figure 5 shows an embodiment 50 of the method that is used with the setup of
figure
3 in some embodiments. The said tree seedlings are predominantly exposed to
the
artificial light spectrum, as seedlings are housed in a dark or shaded growth
cavity 360
in phase 500.
In phase 510 the artificial lighting produces a spectrum that resembles
sunlight, as the
artificial light is nearly the sole source of light. In some embodiments the
artificial
light produces short pulses of UV from a location that is very close to the
seedling to
acclimatize the seedlings to outdoor life in phase 520. This is preferable in
some
embodiments of the invention as it minimizes the energy used in illumination
and a
CA 3055322 2019-09-12
12
great majority of the high UV photons intended to reach the seedling do reach
the
seedling, rather than being shined off target.
It should also further be noted that the embodiment 50 can be readily permuted
and/or
combined with any of the embodiments 10, 20, 30 and/or 40 and be used to
create any
of the embodiments 10, 20, 30, 40 and/or 60.
It should be noted that the artificial UV light generated to overcome
transplantation
shock can be arranged in various device configurations. In one embodiment the
artificial UV light can be a LED light that emits solely or mainly in the UV-B
band in
accordance with the invention. In other embodiments the UV light is integrated
to and
with other light emitting devices, such as LEDs, that may emit in any of the
following
bands: UV, visible light, far red band (700-800 nm), infra red band (800nm+).
In some embodiments the light is produced by electroluminence or by
phosphorence
or both in accordance with the invention. For example, in one embodiment the
UV
light is produced by electroluminence and the light in the visible or IR band
is
produced by phosphor or phosphorescing material in the proximity of the UV
light
that absorbs the UV light and then emits light of longer wavelengths. In this
phosphorence based embodiment it is possible to adjust the ratio of the
intensities of
UV emission and visible-infrared emission by adjusting the type and quantity
of the
phosphorescing material when the light device is manufactured.
Each LED may have one or more emission peaks in accordance with the invention
both in the UV and in the visible, far red and/or infrared bands in accordance
with the
invention.
Figure 6 shows preferable LED spectra used in accordance with the invention
that
have been built and tested by the applicant. Wavelength is shown on the
horizontal x-
axis and relative intensity in the vertical y-axis. One preferable embodiment
is known
to be a lUV LED + G2 LED of figure 6 or a UV LED and AP9 LED of the applicant
CA 3055322 2019-09-12
= 13
from figure 6. Another preferred embodiment of the invention combines the
spectra
AP6 and AP7 of figure 6 with UV LEDs.
In one embodiment of the invention, the at least one UV LED or LEDs emit in
the
UV-A (315-400 nm) and UV-B (280-315 nm), but not in the UV C (100-280 nm)
bands in accordance with the invention.
It should be noted that any advantageous dosage regime of artificial light to
treat
transplantation shock maybe applied in accordance with the invention. In one
embodiment small exposures of artificial light are administered at known or
random
intervals. In other embodiments the light exposure to treat transplantation
shock is
administered at the end of the indoors growth period in accordance with the
invention.
It should further be noted that it is in accordance with the invention to
combine the
artificial light treatment of the invention with other transplantation shock
treatments,
such as cooling of the seedlings. In some embodiments the artificial lights of
the
invention are housed in a refrigerator, so that seedlings are arranged to be
acclimatised to outdoor winter conditions in the said refrigerator. Similarly,
the
artificial light treatment of transplantation shock, may be combined with
artificial
wind, or controlled diet of minerals for the seedlings in accordance with the
invention.
It should further be noted that in any of the preceding embodiments of the
invention
the light device arranged to produce articial light for the treatment of
transplantation
shock may be equipped with any of the following phosphorescing materials:
- MAlSiN3X (where in M is Metal such as Ca, Sr, Ba and X is rare earth
element such as Eu or Mn in any various ratios and combinations),
- MMgSiOX (where in M is Metal such as Ca, Sr, Ba and X is
rare earth
element such as Eu or Mn in any various ratios and combinations).
The invention has been explained above with reference to the aforementioned
embodiments and several commercial and industrial advantages have been
demonstrated. The methods and arrangements of the invention allow treating
tree
CA 3055322 2019-09-12
14
seedlings against transplantation shock, and thereby increase the likelihood
of a successful
planting of the tree seedling to the outdoors. The treatment of the invention
reduces work phases
for the grower, as the invention removes the need for sunshade curtains during
nursing of the tree
seedlings, and subsequently the need to move the seedlings to and from the
shade area.
The invention has been explained above with reference to the aforementioned
embodiments.
However, it is clear that the invention is not only restricted to these
embodiments but comprises
all possible embodiments within the scope of the invention.
Date Recue/Date Received 2021-03-15
15
REFERENCES
"The physiological basis of containerised tree seedlings 'transplant shock': a
review",
Dugald C. Close, Christopher L Beadle and Philip H. Brown, Australian Forestry
2005, Vol. 68 No. 2 pp. 112-120.
EP 0364952 A2, Determining seed viability, Taylor et al., 1990.
US 2008/0120736, Process of photomorphogenically enhancing plants, William E.
Hurst, 2008.
Photobiology of higher plants, Maurice S. McDonald, John Wiley & Sons, 2003.
CA 3055322 2019-09-12
