Note: Descriptions are shown in the official language in which they were submitted.
TOMATO VARIETY NUN 09247 TOF
FIELD OF THE DISCLOSURE
[1] The disclosure relates to the field of plant breeding and, more
specifically, to the tomato
variety NUN 09247 TOF. The disclosure further relates to vegetative
reproductions of tomato
variety NUN 09247 TOF, methods for tissue culture of tomato variety NUN 09247
TOF, methods
for regenerating a plant from such a tissue culture, and to phenotypic
variants of tomato variety
NUN 09247 TOF.
BACKGROUND
[2] The goal of plant breeding is to combine various desirable traits in a
single variety. Such
desirable traits may include greater yield, resistance to diseases, insects or
other pests, tolerance to
heat and drought, better agronomic quality, higher nutritional value, enhanced
growth rate and
improved fruit properties.
1131 Breeding techniques take advantage of a plant's method of pollination.
There are two
general methods of pollination: self-pollination and cross-pollination. A
plant self-pollinates if
pollen from one flower is transferred to the same or another flower of the
same genotype. A plant
cross-pollinates if pollen comes to it from a flower of a different genotype.
[4] Plants that have been self-pollinated and selected for (uniform) type
over many generations
become homozygous at almost all gene loci and produce a uniform population of
true breeding
progeny of homozygous plants. A cross between two such homozygous plants of
different lines
produces a uniform population of hybrid plants that are heterozygous for many
gene loci. The
extent of heterozygosity in the hybrid is a function of the genetic distance
between the parents.
Conversely, a cross of two plants each heterozygous at a number of loci
produces a segregating
population of hybrid plants that differ genetically and are not uniform. The
resulting non-
uniformity makes performance unpredictable.
151 The development of uniform varieties requires the development of
homozygous inbred
plants, the crossing of these inbred plants to make hybrids, and the
evaluation of the hybrids
resulting from the crosses. Pedigree breeding and recurrent selection are
examples of breeding
methods that have been used to develop inbred plants from breeding
populations. Those breeding
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methods combine the genetic backgrounds from two or more plants or various
other broad-based
sources into breeding pools from which new lines are developed by selfing and
selection of desired
phenotypes. The new plants are evaluated to determine which have commercial
potential.
[6] Tomato (Solanum lycopersicum and closely related species) is naturally
a diploid and the
basic chromosome number of the genus is x=12, most are 2n=2x=24, including the
cultivated ones.
It originated in the New World and has since become a major food crop.
[7] Tomato cultivars may be grouped by maturity, i.e., the time required
from planting the seed
to the stage where fruit harvest can occur. Standard maturity classifications
include 'early',
`midseason' or late-maturing'. Another classification for tomatoes is the
developmental timing of
fruit set. 'Determinate' plants grow foliage, then transition into a
reproductive phase of flower
setting, pollination and fruit development. Consequently, determinant
cultivars have a large
proportion of the fruit ripen within a short time frame. Growers that harvest
only once in a season
favor determinant type cultivars. In contrast, 'indeterminate' types grow
foliage, then enter a long
phase where flower and fruit development proceed along with new foliar growth.
Growers that
harvest the same plants multiple times favor indeterminate type cultivars.
[8] Tomatoes can also be classified by their target markets: fresh market
and processing
tomatoes. Fresh-market tomatoes are primarily used for salads, salad bar and
sandwiches, and
require good storage properties. On the other hand, processing tomatoes
generally requires red
colored and pink to red/crimson fruit flesh and higher percentage of soluble
solids. Processing
tomatoes can be canned whole, canned, diced or chopped, dried, roasted,
pasted, puréed or
concentrated, juiced, frozen, or put into ready- made dishes, for example,
sauces, stews or soups.
[9] In 2017, World Atlas reported that the worldwide production of tomatoes
amounted to
170.8 million tons. United States is ranked as the third largest producer of
tomatoes in the world,
next to China and India. Tomatoes are available in the United States year-
round, with California
and Florida being the major producers. Fresh-market tomatoes are available
from May to
December although supply peaks in July and in September through October.
Processing tomatoes
have the greatest supply from August to September.
[10] In response to more recent consumer demands for dietary diversity, tomato
breeders have
developed a wider range of colors. In addition to expanding the range of red
colored fruits, there
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Date Recue/Date Received 2020-08-31
are cultivars that produce fruits that are creamy white, lime green, yellow,
green, golden, orange
and purple. Additionally, there are multi-colored varieties exemplified by
mainly red fruited
varieties with green shoulders, and both striped- and variegated-colored
fruit.
SUMMARY OF VARIOUS ASPECTS OF THE DISCLOSURE
[11] The disclosure provides for tomato variety NUN 09247 TOF, products
thereof, and
methods of using the same. NUN 09247 TOF is a red cherry saladette (grape)
tomato variety for
the fresh market (snack segment) and is suitable for growing in a protected
(greenhouse)
environment.
[12] In another aspect, the disclosure provides a seed of tomato variety NUN
09247 TOF,
wherein a representative sample of said seed has been deposited under
Accession Number NCIMB
43642. The disclosure also provides for a plurality of seeds of tomato variety
NUN 09247 TOF.
The seed of tomato variety NUN 09247 TOF may be provided as an essentially
homogeneous
population of tomato seed. The population of seed of tomato variety NUN 09247
TOF may be
particularly defined as being essentially free from other seed. The seed
population may be grown
into plants to provide an essentially homogeneous population of tomato plants
as described herein.
[13] The disclosure also provides a plant grown from a seed of tomato variety
NUN 09247 TOF
and a plant part thereof In another aspect, the disclosure provides for a
hybrid tomato variety NUN
09247 TOF. The disclosure also provides for a progeny of tomato variety NUN
09247 TOF. In
another aspect, the disclosure provides a plant or a progeny retaining all or
all but one, two, or
three of the "distinguishing characteristics" or all or all but one, two or
three of the "morphological
and physiological characteristics" of tomato variety NUN 09247 TOF and methods
for producing
that plant or progeny.
[14] In another aspect, the disclosure provides a plant or a progeny having
all the physiological
and morphological characteristics of variety NUN 09247 TOF when grown under
the same
environmental conditions. In another aspect, the plant or progeny has all or
all but one, two, or
three of the physiological and morphological characteristics of tomato variety
NUN 09247 TOF
when measured under the same environmental conditions and e.g., evaluated at
significance levels
of 1%, 5% or 10% significance (which can also be expressed as a p-value) for
quantitative
characteristics and determined by type or degree for non-quantitative
characteristics, wherein a
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Date Recue/Date Received 2020-08-31
representative sample of seed of variety NUN 09247 TOF has been deposited
under Accession
Number NCIMB 43642. In another aspect, the plant or progeny has all or all but
one, two, or three
of the physiological and morphological characteristics as listed in Tables 1
and 2 for variety NUN
09247 TOF when measured under the same environmental conditions and e.g.,
evaluated at
significance levels of 1%, 5% or 10% significance (which can also be expressed
as a p-value) for
quantitative characteristics and determined by type or degree for non-
quantitative characteristics.
[15] In
another aspect, the plant of tomato variety NUN 09247 TOF or a progeny thereof
has
10, 11, or more or all of the following distinguishing characteristics as
shown in Table 3: 1) longer
plant height; 2) horizontal leaf attitude; 3) shorter leaf length; 4) smaller
size of leaflets (in middle
of leaf); 5) horizontal attitude of petiole of leaflet in relation to main
axis; 6) equally uniparous
and multiparous inflorescence type; 7) absence of green shoulder before
maturity; 8) lighter
ripened fruit weight; 9) elliptic fruit shape in longitudinal section; 10)
flat fruit shape at blossom
end; and 11) thicker pericarp, when grown under the same environmental
conditions.
[16] In another aspect, the plant of tomato variety NUN 09247 TOF or a progeny
thereof
comprises resistance to Fulvia fulva Groups A-E and Tomato Mosaic Virus (ToMV)
Strains 0, 1,
2, and 1-2, measured according to UPOV standards described in TG/44/11.
[17] In another aspect, the disclosure provides for a plant part obtained from
tomato variety
NUN 09247 TOF, wherein said plant part is: a fruit, a harvested fruit, a part
of a fruit, a leaf, a part
of a leaf, pollen, an ovule, a cell, a petiole, a shoot or a part thereof, a
stem or a part thereof, a root
or a part thereof, a root tip, a cutting, a seed, a part of a seed, seed coat
or another maternal tissue
which is part of a seed grown on said variety, a hypocotyl, a cotyledon, a
scion, a stock, a rootstock,
a pistil, an anther, or a flower or a part thereof Fruits are particularly
important plant parts. In
another aspect, the plant part obtained from variety NUN 09247 TOF is a cell,
optionally a cell in
a cell or tissue culture. That cell may be grown into a plant of variety NUN
09247 TOF.
[18] The disclosure also provides a cell culture of tomato variety NUN 09247
TOF and a plant
regenerated from tomato variety NUN 09247 TOF, wherein the plant has all the
characteristics of
variety NUN 09247 TOF when grown under the same environmental conditions, as
well as
methods for culturing and regenerating tomato variety NUN 09247 TOF.
Alternatively, a
regenerated plant may have one characteristic that is different from tomato
variety NUN 09247
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TOF and otherwise has all of the physiological and morphological
characteristics of the plant of
tomato variety NUN 09247 TOF.
[19] The disclosure further provides a vegetatively propagated plant of
variety NUN 09247 TOF
having all or all but one, two, or three of the morphological and
physiological characteristics of
tomato variety NUN 09247 TOF when grown under the same environmental
conditions.
[20] The disclosure furthermore provides a tomato fruit produced on a plant
grown from a seed
of variety NUN 09247 TOF.
[21] In another aspect, the disclosure provides a seed growing or grown on a
plant of variety
NUN 09247 TOF (i.e., produced after pollination of the flower of tomato
variety NUN 09247
TOF).
[22] The invention as claimed relates to:
1. A cell of tomato plant variety NUN 09247 TOF, or a cell of a part
thereof, wherein the
representative sample of seed of said tomato variety is deposited under
Accession
Number 43642.
2. A cell of a seed of tomato variety NUN 09247 TOF, wherein a representative
sample
of seed of said tomato variety is deposited under Accession Number 43642.
3. A cell of a plant or a cell of a part thereof, produced by growing the
seed as defined in
embodiment 2.
4. A cell of a seed that produces the plant as defined in embodiment 1.
5. A cell of a plant part of embodiment 1, wherein the plant part is a
leaf, pollen, an ovule,
a fruit, a scion, a root, a rootstock, a cutting, or a flower.
6. The cell of a plant part of embodiment 5, when the plant part is a fruit
or part thereof
7. A cell of a tomato plant having all of the physiological and morphological
characteristics of the tomato plant as defined in embodiment 1.
8. A cell of a tomato plant or a cell of a part thereof, which does not differ
from the
physiological and morphological characteristics of the plant of tomato variety
NUN
Date Recue/Date Received 2020-08-31
09247 TOF listed in Table 1, when grown under the same environmental
conditions,
and wherein a representative sample of seed of said tomato variety is
deposited under
Accession Number NCIMB 43642.
9. A tissue or cell culture of regenerable cells of the plant as defined
in embodiment 1.
10. The tissue or cell culture according to embodiment 9, comprising cells or
protoplast
from a plant part, wherein the plant part is a meristem, a cotyledon, a
hypocotyl, a
pollen, a leaf, an anther, a root, a root tip, a pistil, a petiole, a flower,
a fruit, a stalk, or
a stem.
11. A cell of a tomato plant regenerated from the tissue or cell culture of
embodiment 9,
wherein the plant has all of the physiological and morphological
characteristics of the
plant of tomato variety NUN 09247 TOF, when grown under the same environmental
conditions, and wherein a representative sample of seed of said variety is
deposited
under Accession Number NCIMB 43642.
12. A cell of a tomato plant produced by vegetatively propagating at least a
part of the plant
of tomato variety NUN 09247 TOF, wherein a representative sample of seed of
said
tomato variety is deposited under Accession Number NCIMB 43642.
13. The cell of embodiment 12, wherein said vegetative propagation comprises
regenerating a whole plant from said part of the plant of tomato variety NUN
09247
TOF, wherein a representative sample of seed of said tomato variety is
deposited under
Accession Number NCIMB 43642.
14. The cell of embodiment 12, wherein said part is a cutting, a cell culture,
or a tissue
culture.
15. A cell of a vegetatively propagated tomato plant, or a cell of a part
thereof, wherein the
plant or part thereof have all of the physiological and morphological
characteristics of
the plant of tomato variety NUN 09247 TOF, when grown under the same
environmental conditions, and wherein a representative sample of seed of said
tomato
variety is deposited under Accession Number NCIMB 43642.
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Date Recue/Date Received 2020-08-31
16. A cell of a tomato plant produced by a method comprising crossing the
plant as defined
in embodiment 1 with a second tomato plant at least once, and selecting a
progeny
tomato plant from said crossing and optionally allowing the progeny tomato
plant to
form seed.
17. A cell of a tomato plant produced by a method comprising selfing the plant
as defined
in embodiment 1 one or more times, and selecting a progeny tomato plant from
said
selfing selfing and optionally allowing the progeny tomato plant to form seed.
18. A cell of a tomato plant produced by a method comprising crossing tomato
plants and
harvesting the resultant seed, wherein at least one tomato plant is the plant
as defined
in embodiment 1, wherein a representative sample of seed of said tomato
variety NUN
09247 TOF is deposited under NCIMB Accession Number 43642.
19. A cell of the tomato seed produced by the method as defined in embodiment
18.
20. A cell of the tomato plant or a cell of part thereof produced by a method
comprising
growing the seed as defined in embodiment 19.
21. A cell of a tomato plant having one physiological or morphological
characteristic which
is different from those of the plant as defined in embodiment 1, and which
otherwise
has all of the physiological and morphological characteristics of the plant of
tomato
variety NUN 09247 TOF, when grown under the same environmental conditions, and
wherein a representative sample of seed of said tomato variety is deposited
under
Accession Number NCIMB 43642.
22. A cell of a single locus converted plant of tomato variety NUN 09247 TOF,
wherein
the plant has otherwise all of the physiological and morphological
characteristics of the
plant of tomato variety NUN 09247 TOF when grown under the same environmental
conditions, wherein the single locus is introduced by genetic transformation,
and
wherein a representative sample of seed of said tomato variety is deposited
under
Accession Number NCIMB 43642.
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Date Recue/Date Received 2020-08-31
23. The cell of embodiment 22, wherein the single locus conversion confers
yield, size,
compactness, dry matter content, firmness, flavor, fruit quality, enhanced
nutritional
quality, post-harvest quality, storage properties, color, male sterility,
herbicide
tolerance, insect resistance, pest resistance, disease resistance, Powdery
mildew
resistance without necrosis, environmental stress tolerance, modified
carbohydrate
metabolism, modified protein metabolism, or ripening.
24. A cell of a doubled haploid tomato plant produced by a method of making
doubled
haploids from haploid cells made from the plant of tomato variety NUN 09247
TOF,
wherein a representative sample of seed of said tomato variety is deposited
under
Accession Number NCIMB 43642.
25. A cell of a tomato plant produced by a method comprising transforming the
plant of
tomato variety NUN 09247 TOF with a transgene that confers the desired trait,
wherein
the transformed plant otherwise retains all of the physiological and
morphological
characteristics of tomato variety NUN 09247 TOF and contains the desired
trait,
wherein a representative sample of seed of said tomato variety is deposited
under
Accession Number NCIMB 43642.
26. The cell of embodiment 25, wherein the desired trait is the desired trait
is yield, size,
compactness, dry matter content, firmness, flavor, fruit quality, enhanced
nutritional
quality, post-harvest quality, storage properties, color, male sterility,
herbicide
tolerance, insect resistance, pest resistance, disease resistance, Powdery
mildew
resistance without necrosis, environmental stress tolerance, modified
carbohydrate
metabolism, modified protein metabolism, or ripening.
27. A cell of a tomato plant having a trait, produced by a method comprising
mutating a
plant or plant part tomato variety NUN 09247 TOF and selecting a mutated plant
with
a desired trait, wherein the mutated plant otherwise retains all of the
physiological and
morphological characteristics of tomato variety NUN 09247 TOF, when grown
under
the same environmental conditions, wherein a representative sample of seed of
said
tomato variety is deposited under Accession Number NCIMB 43642.
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Date Recue/Date Received 2020-08-31
28. The cell of embodiment 27, wherein the desired trait is yield, size,
compactness, dry
matter content, firmness, flavor, fruit quality, enhanced nutritional quality,
post-harvest
quality, storage properties, color, male sterility, herbicide tolerance,
insect resistance,
pest resistance, disease resistance, Powdery mildew resistance without
necrosis,
environmental stress tolerance, modified carbohydrate metabolism, modified
protein
metabolism, or ripening.
29. A cell of a tomato plant produced by a method comprising growing a plant
as defined
in embodiment 1 until it sets at least one fruit and collecting the fruit.
30. A method of determining the genotype of the plant as defined in embodiment
1,
comprising obtaining a sample of nucleic acids form said plant and detecting
in said
nucleic acids a plurality of polymorphisms, thereby determining the genotype
of the
plant and storing the results of detecting the plurality of polymorphisms on a
computer
readable medium.
31. A method comprising packaging seeds as defined in embodiment 2 in a
container.
32. A method comprising packaging a tomato fruit as defined in embodiment 5 in
a
container.
33. Use of a tomato plant as defined in embodiment 1 as a scion or a
rootstock.
34. Use of a tomato fruit or part thereof as defined in embodiment 5 as a
food, a feed
product, or a processed product, or to produce a food, a feed, or a processed
product.
35. Use of a tomato plant variety NUN 09247 TOF, or a part thereof, for
producing a seed,
a crop, or a food or a processed product, wherein a representative sample of
seed of
said tomato variety is deposited under Accession Number NCIMB 43642.
36. Use of a seed of tomato variety NUN 09247 TOF, for producing tomato
plants, wherein
a representative sample of seed of said tomato variety is deposited under
Accession
Number NCIMB 43642.
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Date Recue/Date Received 2020-08-31
37. Use of a tomato plant having all of the physiological and morphological
characteristics
of the tomato plant as defined in embodiment 1 for producing a seed, a crop,
or a food
or a processed product.
38. Use, for producing a seed, a crop, or a food or a processed product, of a
tomato plant
or a part thereof which does not differ from the physiological and
morphological
characteristics of tomato variety NUN 09247 TOF CUL listed in Table 1, when
grown
under the same environmental conditions, and wherein a representative sample
of seed
of said tomato variety is deposited under Accession Number NCIMB 43642.
39. Use, for producing a seed, a crop, or a food or a processed product, of a
tomato plant
regenerated from the tissue or cell culture as defined in embodiment 9,
wherein the
plant has all of the physiological and morphological characteristics of tomato
variety
NUN 09247 TOF, when grown under the same environmental conditions, and wherein
a representative sample of seed of said tomato variety is deposited under
Accession
Number NCIMB 43642.
40. Use of a vegetatively propagated plant to produce a seed, a crop, or a
food or a
processed product, wherein the vegetatively propagated plant has all of the
physiological and morphological characteristics of tomato variety NUN 09247
TOF,
when grown under the same environmental conditions, and wherein a
representative
sample of seed of said tomato variety is deposited under Accession Number
NCIMB
43642.
41. Use, for producing a seed, a crop, or a food or a processed product, of a
tomato plant
produced by crossing a plant as defined in embodiment 1 with a second tomato
plant at
least once, and selecting a progeny tomato plant from said crossing and
optionally
allowing the progeny tomato plant to form seed.
42. Use, for producing a seed, a crop, or a food or a processed product, of a
tomato plant
produced by selfing the plant as defined in embodiment 1 one or more times,
and
selecting a progeny tomato plant from said selfing and optionally allowing the
progeny
tomato plant to form seed.
Date Recue/Date Received 2020-08-31
43. Use of the plant of tomato variety NUN 09247 TOF, wherein a representative
sample
of seed of said tomato variety is deposited under NCIMB Accession Number
43642, to
cross with a second tomato plant.
44. Use of the plant of tomato variety NUN 09247 TOF, wherein a representative
sample
of seed of said tomato variety is deposited under NCIMB Accession Number
43642,
and a second tomato plant to produce a tomato seed.
45. Use of the plant of tomato variety NUN 09247 TOF, wherein a representative
sample
of seed of said tomato variety is deposited under NCIMB Accession Number
43642,
and a second tomato plant to produce a descendant plant.
46. Use of tomato variety NUN 09247 TOF, wherein a representative sample of
seed of
said tomato variety is deposited under NCIMB Accession Number 43642, to breed
a
tomato plant.
47. Use, for producing a seed, a crop, or a food or a processed product, of a
tomato plant
having one physiological or morphological characteristic which is different
from those
of the plant as defined in embodiment 1, and which otherwise has all of the
physiological and morphological characteristics of the plant of tomato variety
NUN
09247 TOF, when grown under the same environmental conditions, and wherein a
representative sample of seed of said tomato variety is deposited under
Accession
Number NCIMB 43642.
48. Use, for producing a seed, a crop, or a food or a processed product, of a
single locus
converted plant, wherein the plant has otherwise all of the physiological and
morphological characteristics of the plant of tomato variety NUN 09247 TOF
when
grown under the same environmental conditions, wherein the single locus is
introduced
by genetic transformation, and wherein a representative sample of seed of said
tomato
variety is deposited under Accession Number NCIMB 43642.
49. Use of tomato variety NUN 09247 TOF, and wherein a representative sample
of seed
of said tomato variety is deposited under Accession Number NCIMB 43642, as a
recipient of a single locus conversion.
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Date Recue/Date Received 2020-08-31
50. Use, for producing a seed, a crop, or a food or a processed product, of a
doubled haploid
of tomato variety NUN 09247 TOF produced by making doubled haploids from
haploid
cells made from the plant of tomato variety NUN 09247 TOF, wherein a
representative
sample of seed of said tomato variety is deposited under Accession Number
NCIMB
43642.
51. Use, for producing a seed, a crop, or a food or a processed product, of a
tomato plant
produced by a method comprising transforming the plant of tomato variety NUN
09247
TOF with a transgene that confers the desired trait, wherein the transformed
plant
otherwise retains all of the physiological and morphological characteristics
of tomato
variety NUN 09247 TOF and contains the desired trait, wherein a representative
sample
of seed of said tomato variety is deposited under Accession Number NCIMB
43642.
52. Use of tomato variety NUN 09247 TOF, and wherein a representative sample
of seed
of said tomato variety is deposited under Accession Number NCIMB 43642, a
recipient
of a transgene.
53. Use, for producing a seed, a crop, or a food or a processed product, of a
tomato plant
having a trait, wherein the tomato plant is produced by a method comprising
mutating
a plant or plant part tomato variety NUN 09247 TOF and selecting a mutated
plant with
a desired trait, wherein the mutated plant otherwise retains all of the
physiological and
morphological characteristics of tomato variety NUN 09247 TOF, when grown
under
the same environmental conditions, wherein a representative sample of seed of
said
tomato variety is deposited under Accession Number NCIMB 43642.
BRIEF DESCRIPTION OF DRAWINGS
23] Fig. 1 shows the plant of tomato variety NUN 09247 TOF.
24] Fig. 2 shows the flower and cross-section of mature fruit of tomato
variety NUN 09247
TOF.
ps] Fig. 3 shows the leaf of tomato variety NUN 09247 TOF.
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Date Recue/Date Received 2020-08-31
DEFINITIONS
26] "Tomato" refers herein to plants of the species Solanum lycopersicum,
or a closely related
species, and fruits thereof. Solanum lycopersicum is also known as
Lycopersicon lycopersicum
(L.) H. Karst. or Lycopersicon esculentum Mill. The most commonly eaten part
of a tomato is the
fruit or berry.
[27] "Cultivated tomato" refers to plants of Solanum lycopersicum, or a
closely related species
(e.g., varieties, breeding lines or cultivars of the species S. lycopersicum
as well as crossbreds
thereof, or crossbreds with other Solanum species), cultivated by humans and
having good
agronomic characteristics.
[28] The terms "tomato plant designated NUN 09247 TOF," "NUN 09247 TOF," "NUN
09247," "NUN 09247 Fl," "09247 TOF," "tomato 09247," or "Luvion" are used
interchangeably
herein and refer to a tomato plant of variety NUN 09247 TOF, representative
seed of which is
deposited under Accession Number NCIMB 43642.
29] A "seed of NUN 09247 TOF" refers to a tomato seed which can be grown
into a plant of
variety NUN 09247 TOF, wherein a representative sample of viable seed of
tomato variety NUN
09247 TOF has been deposited under Accession Number NCIMB 43642. A seed can be
in any
stage of maturity, for example, a mature, viable seed, or an immature, non-
viable seed. A seed
comprises an embryo and maternal tissues.
[30] An "embryo of NUN 09247 TOF" refers to an "Fl hybrid embryo" as present
in a seed of
tomato variety NUN 09247 TOF, a representative sample of said seed of tomato
variety NUN
09247 TOF is deposited under Accession Number NCIMB 43642.
[31] A "seed grown on NUN 09247 TOF" refers to a seed grown on a mature plant
of variety
NUN 09247 TOF or inside a fruit of tomato variety NUN 09247 TOF. The "seed
grown on NUN
09247 TOF" contains tissues and DNA of the maternal parent, tomato variety NUN
09247 TOF.
The "seed grown on NUN 09247 TOF" contains an F2 embryo. When said seed is
planted, it grows
into a first generation progeny plant of variety NUN 09247 TOF.
[32] A "fruit of NUN 09247 TOF" refers to a fruit containing maternal tissues
of tomato variety
NUN 09247 TOF as deposited under Accession Number NCIMB 43642. The fruit
comprises
pericarp, septa, epidermis, columella, locular cavity, vascular bundles and
optionally seed.
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Date Recue/Date Received 2020-08-31
Pericarp, septa, epidermis, columella, locular cavity, vascular bundles, and
seed coat of the seed
are maternal tissues, e.g., they are genetically identical to the plant on
which they grow. In one
aspect, the fruit contains seed grown on tomato variety NUN 09247 TOF. In
another aspect, the
fruit does not contain seed, i.e., the fruit is parthenocarpic. The skilled
person is familiar with
methods for inducing parthenocarpy. Those methods comprise chemically or
genetically inducing
parthenocarpy. Compounds suitable for chemically inducing parthenocarpy
comprise auxins,
gibberellins and cytokinins. Methods for genetically inducing parthenocarpy
comprise the
methods described in US 9125353, US 2002/0010953, US 6060648, EP 1057401 and
EP 1428425.
[33] An "essentially homogeneous population of tomato seed" is a population of
seeds where at
least 97%, 98%, 99% or more of the total population of seed are seed of tomato
variety NUN
09247 TOF.
[34] An "essentially homogeneous population of tomato plants" is a population
of plants where
at least 97%, 98%, 99% or more of the total population of plants are plants of
variety NUN 09247
TOF.
[35] The phrase "essentially free from other seed" refers to a population of
seed where less than
3%, 2%, 1% or less of the total population of seed is seed that is not a
tomato seed or, in another
aspect, less than 3%, 2%, 1% or less of the total population of seed is seed
that is not seed of
tomato variety NUN 09247 TOF.
[36] "Tissue
culture" or "cell culture" refers to a composition comprising isolated cells
of the
same or a different type or a collection of such cells organized into parts of
a plant. Tissue culture
of various tissues of tomato and regeneration of plants therefrom is well
known and widely
published (see, e.g., Bhatia et al. (2004), Plant Cell, Tissue and Organ
Culture 78: 1-21). Similarly,
methods of preparing cell cultures are known in the art.
[37] "USDA descriptors" are the plant variety descriptors described for
tomatoing the
"Objective Description of Variety Tomato (Solanum lycopersicum or Lycopersicon
esculentum
Mill)", as published by the US Department of Agriculture, Agricultural
Marketing Service, Plant
Variety Protection Office, Beltsville. "Non-USDA descriptors" are other
descriptors suitable for
describing tomato.
14
Date Recue/Date Received 2020-08-31
[38] "UPOV descriptors" are the plant variety descriptors described for tomato
in the
"Guidelines for the Conduct of Tests for Distinctness, Uniformity and
Stability, TG/44/11 (Geneva
2011, revised 2013-03-20), as published by UPOV (International Union for the
Protection of New
Varieties and Plants). Likewise, "UPOV methods" to determine specific
parameters for the
characterization of tomato are described at upov.int.
[39] "RHS"
refers to the Royal Horticultural Society (RHS) which publishes an official
botanical color chart quantitatively identifying colors according to a defined
numbering system.
The chart may be purchased from Royal Horticulture Society Enterprise Ltd RHS
Garden; Wisley,
Woking; Surrey GU236QB, UK, e.g., the RHS color chart 2007.
[40] "Reference Variety for NUN 09247 TOF" refers herein to variety Sweetelle
from Syngenta,
which has been planted in a trial together with NUN 09247 TOF. The
characteristics of tomato
variety NUN 09247 TOF are compared with the characteristics of the Reference
Variety as shown
in Tables 1 and 2. The distinguishing characteristics between tomato variety
NUN 09247 TOF and
the Reference Variety are shown in Table 3.
[41] "Plant part" includes any part of a plant, such as a plant organ (e.g.,
harvested or non-
harvested fruits), a plant cell, a plant protoplast, a plant cell tissue
culture or a tissue culture from
which a whole plant can be regenerated, a plant cell that is intact in a
plant, a clone, a
micropropagation, plant callus, a plant cell clump, a plant transplant, a
vegetative propagation, a
seedling, a fruit, a harvested fruit, a part of a fruit, a leaf, a part of a
leaf, pollen, an ovule, an
embryo, a petiole, a shoot or a part thereof, a stem or a part thereof, a root
or a part thereof, a root
tip, a cutting, a seed, a part of a seed, hypocotyl, cotyledon, a scion, a
graft, a stock, a rootstock, a
pistil, an anther, and a flower or parts of any of these and the like. Seed
can be mature or immature.
Pollen or ovules may be viable or non-viable. Also, any developmental stage is
included, such as
seedlings, cuttings prior or after rooting, mature plants or leaves.
Alternatively, a plant part may
also include a plant seed which comprises one or two sets of chromosomes
derived from the parent
plant, e.g., from tomato variety NUN 09247 TOF. An Fl progeny produced from
self-pollination
of tomato variety NUN 09247 TOF will thus comprise two sets of chromosomes
derived from
tomato variety NUN 09247 TOF, while an Fl progeny derived from cross-
fertilization of tomato
variety NUN 09247 TOF will comprise only one set of chromosomes from tomato
variety NUN
09247 TOF, and the other set of chromosomes from the other parent.
Date Recue/Date Received 2020-08-31
[42]
"Harvest maturity" is referred to as the stage at which a tomato fruit is ripe
or ready for
harvest or the optimal time to harvest the fruit for the market, for
processing or for consumption.
In one aspect, harvest maturity is the stage which allows proper completion of
the normal ripening.
[43] "Harvested plant material" refers herein to plant parts (e.g., single
fruits or clusters of fruits
detached from the whole plant), which have been collected for further storage
and/or further use.
[44] "Yield" means the total weight of all tomato fruits harvested per hectare
of a particular line
or variety. It is understood that "yield" expressed as weight of all tomato
fruits harvested per
hectare can be obtained by multiplying the number of plants per hectare times
the "yield per plant".
[45] "Marketable yield" means the total weight of all marketable tomato
fruits, especially fruit
which is not cracked, damaged or diseased, harvested per hectare of a
particular line or variety. A
"marketable fruit" is a fruit that has commercial value.
[46] "Rootstock" or "stock" refers to the plant selected for its roots, in
particular for the
resistance of the roots to diseases or stress (e.g., heat, cold, salinity
etc.). Normally the quality of
the fruit of the plant providing the rootstock is less important.
[47] "Scion" refers to a part of the plant attached to the rootstock. This
plant is selected for its
stems, leaves, flowers, or fruits. The scion contains the desired genes to be
duplicated in future
production by the stock/scion plant and may produce the desired tomato fruit.
[48] "Stock/scion" or grafted plant refers to a tomato plant comprising a
rootstock from one
plant grafted to a scion from another plant.
[49] A plant having "all the physiological and morphological characteristics"
of a referred-to-
plant means a plant showing the physiological and morphological
characteristics of the referred-
to-plant when grown under the same environmental conditions, preferably in the
same experiment;
the referred-to-plant can be a plant from which it was derived, e.g., the
progenitor plant, the parent,
the recurrent parent, the plant used for tissue- or cell culture, etc. A
physiological or morphological
characteristic can be a numerical characteristic or a non-numerical
characteristic. In one aspect, a
plant has "all but one, two or three of the physiological and morphological
characteristics" of a
referred-to-plant, or "all the physiological and morphological
characteristics" of Tables 1 and 2 or
"all or all but one, two or three of the physiological and morphological
characteristics" of Tables
1 and 2.
16
Date Recue/Date Received 2020-08-31
[50] The physiological and/or morphological characteristics mentioned above
are commonly
evaluated at significance levels of 1%, 5% or 10% if they are numerical
(quantitative), or for
having an identical degree (or type) if not numerical (not quantitative), if
measured under the same
environmental conditions. For example, a progeny plant or a Single Locus
Converted plant or a
mutated plant of variety NUN 09247 TOF may have one or more (or all) of the
essential
physiological and/or morphological characteristics of said variety listed in
Tables 1 and 2, as
determined at the 5% significance level (i.e., p <0.05) for numerical
characteristics and determined
by type or degree for non-numerical characteristics, when grown under the same
environmental
conditions.
[51] "Distinguishing characteristics" or "distinguishing morphological and/or
physiological
characteristics" refers herein to the characteristics which distinguish (i.e.,
are different) between
the new variety and other tomato varieties, such as the Reference Variety,
when grown under the
same environmental conditions. The distinguishing characteristics between
tomato variety NUN
09247 TOF and Reference Variety are described in Table 3. When comparing
tomato variety NUN
09247 TOF with different varieties, the distinguishing characteristics may be
different. In one
aspect, the distinguishing characteristics may therefore include at least one,
two, three or more (or
all) of the characteristics listed in Tables 1 and 2. All numerical
distinguishing characteristics are
statistically significantly different at p < 0.05 between tomato variety NUN
09247 TOF and the
other variety (e.g., Reference Variety).
[52] Tomato variety NUN 09247 TOF has the following distinguishing
characteristics when
compared to the Reference Variety as shown in Table 3: 1) longer plant height;
2) horizontal leaf
attitude; 3) shorter leaf length; 4) smaller size of leaflets (in middle of
leaf); 5) horizontal attitude
of petiole of leaflet in relation to main axis; 6) equally uniparous and
multiparous inflorescence
type; 7) absence of green shoulder before maturity; 8) lighter ripened fruit
weight; 9) elliptic fruit
shape in longitudinal section; 10) flat fruit shape at blossom end; and 11)
thicker pericarp, when
grown under the same environmental conditions.
[53] Thus, a tomato plant "comprising the distinguishing characteristics of
tomato variety NUN
09247 TOF" (such as a progeny plant) refers herein to a plant which does not
differ significantly
from said variety in the distinguishing characteristics above. Therefore, in
one aspect, the
17
Date Recue/Date Received 2020-08-31
disclosure provides a plant that does not differ significantly from tomato
variety NUN 09247 TOF
in the distinguishing characteristics above.
[54]
Similarity and differences between two different plant lines or varieties can
be determined
by comparing the number of morphological and/or physiological characteristics
(e.g., the
characteristics as listed in Tables 1 and 2) that are the same (i.e.,
statistically not significantly
different) or that are different (i.e., statistically significantly different)
between the two plant lines
or varieties when grown under the same environmental conditions. A numerical
characteristic is
considered to be "the same" when the value for a numeric characteristic is not
significantly
different at the 1% (p < 0.01) or 5% (p < 0.05) significance level, using one
way analysis of
variance (ANOVA), a standard method known to the skilled person. Non-numerical
or "degree"
or "type" characteristic is considered "the same" when the values have the
same "degree" or "type"
when scored using USDA and/ or UPOV descriptors, if the plants are grown under
the same
environmental conditions.
[55] A "plant line" is, for example, a breeding line which can be used to
develop one or more
varieties. A breeding line is typically highly homozygous.
[56] "Hybrid variety" or "Fl hybrid" refers to the seeds harvested from
crossing two inbred
(nearly homozygous) parental lines. For example, the female parent is
pollinated with pollen of
the male parent to produce hybrid (F1) seeds on the female parent.
[57] "Regeneration" refers to the development of a plant from cell culture or
tissue culture or
vegetative propagation.
[58] "Vegetative propagation", "vegetative reproduction" or "clonal
propagation" are used
interchangeably herein and mean a method of taking a part of a plant and
allowing that plant part
to form at least roots, and also refer to the plant or plantlet obtained by
that method. Optionally,
the vegetative propagation is grown into a mature plant. The skilled person is
aware of what plant
parts are suitable for use in the method.
[59] "Selfing" refers to self-pollination of a plant, i.e., the transfer of
pollen from the anther to
the stigma of the same plant.
[60] "Crossing" refers to the mating of two parent plants. The term
encompasses "cross-
pollination" and "selfing".
18
Date Recue/Date Received 2020-08-31
[61] "Cross-pollination" refers to the fertilization by the union of two
gametes from different
plants.
[62] As used herein, the terms "resistance" and "tolerance" are used
interchangeably to describe
plants that show no symptoms or significantly reduced symptoms to a specified
biotic pest,
pathogen, abiotic influence or environmental condition compared to a
susceptible plant. These
terms are optionally also used to describe plants showing some symptoms but
that are still able to
produce marketable product with an acceptable yield.
[63] The term "traditional breeding techniques" encompasses herein crossing,
selfing, selection,
doubled haploid production, embryo rescue, protoplast fusion, marker assisted
selection, mutation
breeding, etc. as known to the breeder (e.g., methods other than genetic
modification/transformation/transgenic methods), by which, for example, a
genetically heritable
trait can be transferred from one tomato line or variety to another.
[64] "Backcrossing" is a traditional breeding technique used to introduce a
trait into a plant line
or variety. The plant containing the trait is called the donor plant and the
plant into which the trait
is transferred is called the recurrent parent. An initial cross is made
between the donor parent and
the recurrent parent to produce a progeny plant. Progeny plants which have the
trait are then
crossed to the recurrent parent. After several generations of backcrossing
and/or selfing the
recurrent parent comprises the trait of the donor. The plant generated in this
way may be referred
to as a "single trait converted plant". The technique can also be used on a
parental line of a hybrid.
[65] "Progeny" as used herein refers to a plant obtained from a plant variety
designated NUN
09247 TOF. A progeny may be obtained by regeneration of cell culture or tissue
culture or parts
of a plant of said variety or selfing of a plant of said variety or by
producing seeds of a plant of
said variety. In further aspects, progeny may also encompass plants obtained
from crossing of at
least one plant of said variety with another tomato plant of the same variety
or another variety or
(breeding) line, or with wild tomato plants. A progeny may comprise a mutation
or a transgene. A
"first generation progeny" or is the progeny directly derived from, obtained
from, obtainable from
or derivable from the parent plant by, e.g., traditional breeding methods
(selfing and/or cross-
pollinating) or regeneration (optimally combined with transformation or
mutation). Thus, a plant
of tomato variety NUN 09247 TOF is the male parent, the female parent or both
of a first
generation progeny of that variety. Progeny may have all the physiological and
morphological
19
Date Recue/Date Received 2020-08-31
characteristics of tomato variety NUN 09247 TOF when grown under the same
environmental
conditions. Using common breeding methods such as backcrossing or recurrent
selection, mutation
or transformation, one or more specific characteristics may be introduced into
said variety, to
provide a plant comprising all but 1, 2, or 3 or more of the morphological and
physiological
characteristics of variety NUN 09247 TOF (e.g., as listed in Tables 1 and 2).
[66] The terms "gene converted" or "conversion plant" or "single locus
converted plant" in this
context refer to tomato plants which are developed by traditional breeding
techniques e.g.,
backcrossing, or via genetic engineering or through mutation breeding, wherein
essentially all of
the desired morphological and physiological characteristics of the parent
variety or line are
recovered, in addition to the one or more genes transferred into the parent
via e.g., the backcrossing
technique (optionally including reverse breeding or reverse synthesis of
breeding lines). It is
understood that not only the addition of a further characteristic (e.g.,
addition of gene conferring a
further characteristic, such as a disease resistance gene), but also the
replacement/modification of
an existing characteristic by a different characteristic is encompassed herein
(e.g., mutant allele of
a gene can modify the phenotype of a characteristic).
[67] Likewise, a "Single Locus Converted (Conversion) Plant" refers to plants
developed by
plant breeding techniques comprising or consisting of mutation and/or by
genetic transformation
and/or by traditional breeding techniques, such as backcrossing, wherein
essentially all of the
desired morphological and physiological characteristics of a tomato variety
are recovered in
addition to the characteristics of the single locus having been transferred
into the variety via the
above mentioned technique. In case of a hybrid, the gene may be introduced in
the male or female
parental line.
[68] "Average" refers herein to the arithmetic mean.
[69] The term "mean" refers to the arithmetic mean of several measurements.
The skilled person
understands that the appearance of a plant depends to some extent on the
growing conditions of
said plant. Thus, the skilled person will know typical growing conditions for
tomato variety NUN
09247 TOF. The mean, if not indicated otherwise within this application,
refers to the arithmetic
mean of measurements on at least 10 different, randomly selected plants of a
variety or line.
DETAILED DESCRIPTION OF VARIOUS ASPECTS OF THE DISCLOSURE
Date Recue/Date Received 2020-08-31
[70] The disclosure also relates to a plant of variety NUN 09247 TOF, wherein
a representative
sample of seeds of said variety has been deposited under the Budapest Treaty,
with Accession
number NCIMB 43642. NUN 09247 TOF is a red cherry saladette (grape) tomato
variety for the
fresh market (snack segment) and is suitable for growing in a protected
(greenhouse) environment.
[71] The disclosure also relates to a seed of tomato variety NUN 09247 TOF,
wherein a
representative sample of said seed has been deposited under the Budapest
Treaty, with Accession
number NCIMB 43642.
[72] In another aspect, the disclosure provides for a tomato plant part of
variety NUN 09247
TOF, preferably a fruit, a representative sample of seed from said variety is
deposited under the
Budapest Treaty, with Accession number NCIMB 43642.
[73] In another aspect, a seed of hybrid variety NUN 09247 TOF is obtainable
by crossing the
male parent of said variety with the female parent of said variety and
harvesting the seeds produced
on the female parent. The resultant seeds of said variety can be grown to
produce plants of said
variety. In one aspect, a seed or a plurality of seeds of said variety are
packaged into a container
of any size or type (e.g., bags, cartons, cans, etc.). The seed may be
disinfected, primed and/or
treated with various compounds, such as seed coatings or crop protection
compounds. The seed
produces a plant of variety NUN 09247 TOF.
[74] Also provided is a plant of tomato variety NUN 09247 TOF, or a fruit or
other plant part
thereof, produced from a seed, wherein a representative sample of said seeds
is deposited under
the Budapest Treaty, with Accession Number NCIMB 43642.
[75] Also provided is a plant part obtained from variety NUN 09247 TOF,
wherein said plant
part is a fruit, a harvested fruit, a part of a fruit, a leaf, a part of a
leaf, pollen, an ovule, a cell, a
petiole, a shoot or a part thereof, a stem or a part thereof, a root or a part
thereof, a root tip, a
cutting, a seed, a part of a seed, seed coat or another maternal tissue which
is part of a seed grown
on said variety, a hypocotyl, a cotyledon, a scion, a stock, a rootstock, a
pistil, an anther, and a
flower or a part thereof Fruits are particularly important plant parts. Fruits
may be parthenocarpic,
or seedless, or contain immature and/or nonviable seeds. In a further aspect,
the plant part obtained
from variety NUN 09247 TOF is a cell, optionally a cell in a cell or tissue
culture. That cell may
be grown into a plant of variety NUN 09247 TOF. A part of the plant of variety
NUN 09247 TOF
(or of progeny of that variety or of a plant having all physiological and/or
morphological
21
Date Recue/Date Received 2020-08-31
characteristics but one, two or three which are different from those of tomato
variety NUN 09247
TOF) further encompasses any cells, tissues, or organs obtainable from the
seedlings or plants in
any stage of maturity.
[76] The disclosure also provides for a food, a feed, or a processed product
comprising or
consisting of a plant part described herein. Preferably, the plant part is a
tomato fruit or part thereof
and/or an extract from a fruit or another plant part described herein
comprising at least one cell of
tomato variety NUN 09247 TOF. The food or feed product may be fresh or
processed, e.g., dried,
grinded, powdered, pickled, chopped, cooked, roasted, in a sauce, in a
sandwich, pasted, puréed
or concentrated, juiced, pickled, canned, steamed, boiled, fried, blanched
and/or frozen, etc.
[77] Such a plant part of variety NUN 09247 TOF can be stored and/or processed
further. The
disclosure thus also provides for a food or feed product comprising one or
more of such parts, such
as canned, chopped, cooked, roasted, in a sauce, in a sandwich, pasted, puréed
or concentrated,
juiced, frozen, dried, pickled, or powdered tomato fruit from variety NUN
09247 TOF or from
progeny of said variety, or from a derived variety, such as a plant having all
but one, two or three
physiological and/or morphological characteristics of tomato variety NUN 09247
TOF.
[78] In another aspect, the disclosure provides for a tomato fruit of variety
NUN 09247 TOF, or
a part of a fruit of said variety. The fruit can be in any stage of maturity,
for example, immature or
mature. In another aspect, the disclosure provides for a container comprising
or consisting of a
plurality of harvested tomato fruits or parts of fruits of said variety, or
fruits of progeny thereof, or
fruits of a derived variety.
[79] In another aspect, the plant, plant part or seed of tomato variety NUN
09247 TOF is inside
a container, for example, containers such as cans, boxes, crates, bags,
cartons, Modified
Atmosphere Packaging, films (e.g., biodegradable films), etc. comprising a
plant or a part of a
plant (fresh and/or processed) or a seed of tomato variety NUN 09247 TOF. In a
particular aspect,
the container comprises a plurality of seeds of tomato variety NUN 09247 TOF
or a plurality of
plant parts of tomato variety NUN 09247 TOF.
[80] The disclosure further relates to a tomato variety, referred to as NUN
09247 TOF, which
when compared to its Reference Variety has the following distinguishing
characteristics as shown
in Table 3: 1) longer plant height; 2) horizontal leaf attitude; 3) shorter
leaf length; 4) smaller size
of leaflets (in middle of leaf); 5) horizontal attitude of petiole of leaflet
in relation to main axis;
22
Date Recue/Date Received 2020-08-31
6) equally uniparous and multiparous inflorescence type; 7) absence of green
shoulder before
maturity; 8) lighter ripened fruit weight; 9) elliptic fruit shape in
longitudinal section; 10) flat fruit
shape at blossom end; and 11) thicker pericarp, when grown under the same
environmental
conditions. Also encompassed by the present disclosure are parts of that
plant.
[81] In another aspect, the plant of variety NUN 09247 TOF or a progeny plant
thereof,
comprises all of the following morphological and/or physiological
characteristics (i.e., as indicated
on the UPOV Test Guidelines for tomato) as shown in Tables 1 and 2, when grown
under the same
environmental conditions. A part of this plant is also provided.
[82] In another aspect, tomato variety NUN 09247 TOF or a progeny thereof
comprises
resistance to Fulvia fulva Groups A-E and Tomato Mosaic Virus Strain 0, 1, 2,
and 1-2, measured
according to UPOV standards described in TG/44/11.
[83] The disclosure further provides a tomato plant which does not differ from
the physiological
and morphological characteristics of the plant of variety NUN 09247 TOF as
determined at the
1%, 2%, 3%, 4% or 5% significance level for numerical characteristics and
determined by type or
degree for non-numerical characteristics, when grown under the same
environmental conditions.
In a particular aspect, the plants are measured in the same trial (e.g., the
trial is conducted as
recommended by USDA or UPOV). The disclosure also comprises a part of said
plant.
[84] The disclosure also provides a tissue or cell culture comprising cells of
tomato variety NUN
09247 TOF. Such a tissue culture can, for example, be grown on plates or in
liquid culture, or be
frozen for long term storage. The cells of tomato variety NUN 09247 TOF used
to start the culture
can be selected from any plant part suitable for vegetative reproduction, or,
in a particular aspect,
can be cells of an embryo, meristem, a cotyledon, a hypocotyl, pollen, a leaf,
an anther, a root, a
root tip, a pistil, a petiole, a flower, a fruit, seed, or a stem. In another
particular aspect, the tissue
culture does not contain somaclonal variation or has reduced somaclonal
variation. The skilled
person is familiar with methods to reduce or prevent somaclonal variation,
including regular
reinitiation.
[85] In another aspect, the disclosure provides a tomato plant regenerated
from the tissue or cell
culture of tomato variety NUN 09247 TOF, wherein the regenerated plant is not
significantly
different from tomato variety NUN 09247 TOF in all, or all but one, two, or
three, of the
physiological and morphological characteristics (e.g., determined at the 5%
significance level
23
Date Recue/Date Received 2020-08-31
when grown under the same environmental conditions). Optionally, the plant has
one, two, or three
the physiological and morphological characteristics that are affected by a
mutation or by
transformation. In another aspect, the disclosure provides a tomato plant
regenerated from the
tissue or cell culture of tomato variety NUN 09247 TOF, wherein the plant has
all of the
physiological and morphological characteristics of said variety determined
(e.g., 5% significance
level) when grown under the same environmental conditions. Similarity or
difference of a
characteristic is determined by measuring the characteristics on a
representative number of plants
grown under the same environmental conditions, determining whether type/degree
characteristics
are the same and determining whether numerical characteristics are different
at the 5% significance
level.
[86] Tomato variety NUN 09247 TOF, or its progeny, or a plant having all
physiological and/or
morphological characteristics but one, two, or three which are different from
those of tomato
variety NUN 09247 TOF can also be reproduced using vegetative reproduction
methods.
Therefore, the disclosure provides for a method of producing a plant or plant
part of tomato variety
NUN 09247 TOF, comprising vegetative propagation of tomato variety NUN 09247
TOF.
Vegetative propagation comprises regenerating a whole plant from a plant part
of variety NUN
09247 TOF, from a progeny or from or a plant having all physiological and/or
morphological
characteristics of said variety but one, two or three different
characteristics, such as a cutting, a
cell culture or a tissue culture.
[87] The disclosure also provides methods of vegetatively propagating a part
of the plant of
variety NUN 09247 TOF. In certain aspects, the method comprises: (a)
collecting tissue or cells
capable of being propagated from tomato variety NUN 09247 TOF; (b) cultivating
said tissue or
cells to obtain proliferated shoots; and (c) rooting said proliferated shoots,
to obtain rooted
plantlets. Steps (b) and (c) may also be reversed, i.e., first cultivating
said tissue to obtain roots
and then cultivating the tissue to obtain shoots, thereby obtaining rooted
plantlets. The rooted
plantlets may then be further grown, to obtain plants. In one embodiment, the
method further
comprises step (d) growing plants from said rooted plantlets. Therefore, the
method also comprises
regenerating a whole plant from said part of the plant of variety NUN 09247
TOF. In a particular
aspect, the part of the plant to be propagated is a cutting, a cell culture or
a tissue culture.
24
Date Recue/Date Received 2020-08-31
[88] The disclosure also provides for a vegetatively propagated plant of
variety NUN 09247
TOF (or from progeny of tomato variety NUN 09247 TOF or from or a plant having
all but one,
two, or three physiological and/or morphological characteristics of that
variety), wherein the plant
has all of the morphological and physiological characteristics of tomato
variety NUN 09247 TOF,
when the numerical characteristics are determined at the 5% significance level
and determined by
type or degree for non-numerical characteristics for plants grown under the
same environmental
conditions. In another aspect, the propagated plant has all but one, two or
three of the
morphological and physiological characteristics of tomato variety NUN 09247
TOF, when the
numerical characteristics are determined at the 5% significance level and
determined by type or
degree for non-numerical characteristics for plants grown under the same
environmental
conditions. A part of said propagated plant or said propagated plant with one,
two, or three
differences is also provided.
[89] In another aspect, the disclosure provides a method for producing a
tomato plant part,
preferably a fruit, comprising: growing a plant of variety NUN 09247 TOF until
it sets at least one
fruit, and collecting the fruit. Preferably, the fruit is collected at harvest
maturity. In another
embodiment, the fruit is collected when the seed is ripe. A plant of variety
NUN 09247 TOF can
be produced by seeding directly in the soil (e.g., field) or by germinating
the seeds in controlled
environment conditions (e.g., greenhouses, hydroponic cultures, etc.) and
optionally then
transplanting the seedlings into the field. For example, the seed can be sown
into prepared seed
beds where they will remain for the entire production the crop (see, e.g.,
Hartz, et. al., 1996,
University of California, Division of Agriculture and Natural Resources,
Publication 7228, 1-5).
Tomatoes can be grown with a support system such as poles (i.e., stakes) to
keep the fruit from
touching the ground or as bushes without support. Alternatively, plastic row
covers can also be
used to control the temperature. Mulches or plastic tunnels can also be used
to protect the plant
from frost (see, e.g., Strange, et. al., 2000, University of California,
Division of Agriculture and
Natural Resources, Publication 7228, 1-5 ). Tomato can also be grown entirely
in greenhouses.
Moreover, said variety can be grown in hydroponic cultures as described herein
in, e.g., US
2008/0222949, and the skilled person is familiar with various type of
hydroponic cultures.
[90] In still another aspect, the disclosure provides a method of producing a
tomato plant,
comprising crossing a plant of tomato variety NUN 09247 TOF with a second
tomato plant at least
once, allowing seed to develop and optionally harvesting said progeny seed.
The skilled person
Date Recue/Date Received 2020-08-31
can select progeny from said crossing. Optionally, the progeny (grown from the
progeny seed) is
crossed twice, thrice, or four, five, six or seven times, and allowed to set
seed. In one aspect, the
first "crossing" further comprises planting seeds of a first and a second
parent tomato plant, often
in proximity so that pollination will occur; for example, mediated by insect
vectors. Alternatively,
pollen can be transferred manually. Where the plant is self-pollinated,
pollination may occur
without the need for direct human intervention other than plant cultivation.
After pollination the
plant can produce seed.
[91] In still another aspect, the disclosure provides a method of producing a
plant, comprising
selfing a plant of variety NUN 09247 TOF one or more times, and selecting a
progeny tomato
plant from said selfing. In one aspect, the progeny plant retains all the
distinguishing characteristics
of tomato variety NUN 09247 TOF described above when grown under the same
environmental
conditions. In a different aspect, the progeny plant comprises all of the
physiological and
morphological characteristic of tomato variety NUN 09247 TOF of Tables 1 and
2.
[92] In other aspects, the disclosure provides a progeny plant of variety NUN
09247 TOF such
as a progeny plant obtained by further breeding of tomato variety NUN 09247
TOF. Further
breeding with tomato variety NUN 09247 TOF includes selfing that variety one
or more times
and/or cross-pollinating tomato variety NUN 09247 TOF with another tomato
plant or variety one
or more times. In particular, the disclosure provides for a progeny plant that
retains all the essential
morphological and physiological characteristics of tomato variety NUN 09247
TOF or, in another
aspect, a progeny plant that retains all, or all but one, two or three, of the
morphological and
physiological characteristics of tomato variety NUN 09247 TOF, optionally all
or all but one, two
or three of the characteristics as listed in Tables 1 and 2, when grown under
the same environmental
conditions, determined at the 5% significance level for numerical
characteristics and determined
by type or degree for non-numerical characteristics. In a particular aspect,
the progeny is a first
generation progeny, i.e., the ovule or the pollen (or both) used in the
crossing is an ovule or pollen
of variety NUN 09247 TOF, where the pollen comes from an anther and the ovule
comes from an
ovary of variety NUN 09247 TOF. In another aspect, the disclosure provides for
a vegetative
reproduction of the variety and a plant having all, or all but 1, 2, or 3 of
the physiological and
morphological characteristics of tomato variety NUN 09247 TOF (e.g., as listed
in Tables 1 and
2).
26
Date Recue/Date Received 2020-08-31
[93] The disclosure also provides a method for collecting pollen of tomato
variety NUN 09247
TOF, comprising collecting pollen from a plant of variety NUN 09247 TOF.
Alternatively, the
method comprises growing a plant of variety NUN 09247 TOF until at least one
flower contains
pollen and collecting the pollen. In a particular aspect, the pollen is
collected when it is mature or
ripe. A suitable method for collecting pollen comprises collecting anthers or
the part of the anther
that contains pollen, for example, by cutting the anther or the part of the
anther off. Pollen can be
collected in a container. Optionally, collected pollen can be used to
pollinate a tomato flower.
[94] The morphological and/or physiological differences between two different
individual
plants described herein (e.g., between tomato variety NUN 09247 TOF and a
progeny of said
variety) or between a plant of variety NUN 09247 TOF or progeny of said
variety, or a plant having
all, or all but 1, 2, or 3, of the physiological and morphological
characteristics of tomato variety
NUN 09247 TOF (or all, or all but 1, 2, or 3 of the characteristics as listed
in Tables 1 and 2) and
another known variety can easily be established by growing said variety next
to each other or next
to the other variety (in the same field, under the same environmental
conditions), preferably in
several locations which are suitable for said tomato cultivation, and
measuring morphological
and/or physiological characteristics of a number of plants (e.g., to calculate
an average value and
to determine the variation range/uniformity within the variety). For example,
trials can be carried
out in Acampo CA, USA (N 38 degrees 07'261" / W 121 degrees 18' 807", USA),
whereby various
characteristics, for example, maturity, days from seeding to harvest, plant
habit, plant attitude, leaf
shape, leaf color, blistering, numbers of flowers per leaf axil, number of
calyx lobes, number of
petals, fruit group, immature fruit color, mature fruit color, pungency,
flavor, fruit glossiness, fruit
size, fruit shape, average number of fruits per plant, seed size, seed weight,
anthocyanin level,
disease resistance, insect resistance, can be measured and directly compared
for species of tomato.
Thus, the disclosure comprises tomato plant having one, two or three
physiological and/or
morphological characteristics which are different from those of the plant of
variety NUN 09247
TOF and which otherwise has all the physiological and morphological
characteristics of the plant
of variety NUN 09247 TOF, when determined at the 5% significance level for
numerical
characteristics and determined by type or degree for non-numerical
characteristics for plants grown
under the same environmental conditions. In one aspect, the different
characteristic(s) is/are result
of breeding with tomato variety NUN 09247 TOF and selection of a progeny plant
comprising 1,
2 or 3 characteristics which are different than in tomato variety NUN 09247
TOF. In another
27
Date Recue/Date Received 2020-08-31
aspect, the different characteristic is the result of a mutation (e.g.,
spontaneous mutation or a
human induced mutation through e.g., targeted mutagenesis or traditional
mutagenesis such as
chemically or radiation induced mutagenesis), or it is the result of
transformation.
[95] The morphological and physiological characteristics (and the
distinguishing
characteristics) of tomato variety NUN 09247 TOF are provided in Tables 1 and
2. Encompassed
herein is also a plant obtainable from tomato variety NUN 09247 TOF (e.g., by
selfing and/or
crossing and/or backcrossing with said variety and/or progeny of said variety)
comprising all or
all but one, two or three of the physiological and morphological
characteristics of tomato variety
NUN 09247 TOF listed in Tables 1 and 2 as determined at the 5% significance
level for numerical
characteristics and determined by type or degree for non-numerical
characteristics, when grown
under the same environmental conditions and/or comprising one or more (or all;
or all except one,
two or three) characteristics when grown under the same environmental
conditions. The
morphological and/or physiological characteristics may vary somewhat with
variation in the
environment (such as temperature, light intensity, day length, humidity, soil,
fertilizer use), which
is why a comparison under the same environmental conditions is preferred.
Colors can best be
measured using the Royal Horticultural Society (RHS) Chart.
[96] In yet a further aspect, the disclosure provides for a method of
producing a new tomato
plant. The method comprises crossing tomato variety NUN 09247 TOF, or a plant
comprising all
but 1, 2, or 3 of the morphological and physiological characteristics of
tomato variety NUN 09247
TOF (e.g., as listed in Tables 1 and 2), or a progeny plant thereof, either as
male or as female
parent, with a second tomato plant (or a wild relative of tomato) one or more
times, and/or selfing
a tomato plant of variety NUN 09247 TOF, or a progeny plant thereof, one or
more times, and
selecting progeny from said crossing and/or selfing. The second tomato plant
may, for example,
be a line or variety of the species Solanum Lycopersicon, S. chilense, S.
habrochaites, S. penelli,
S. peruvianum, S. pimpinellifolium or other Solanum species.
[97] The disclosure provides for methods of producing plants which retain all
the morphological
and physiological characteristics of a plant described herein. The disclosure
also provides for
methods of producing a plant comprising all but 1, 2, or 3 or more of the
morphological and
physiological characteristics of tomato variety NUN 09247 TOF (e.g., as listed
in Tables 1 and 2),
but which are still genetically closely related to said variety. The
relatedness can, for example, be
28
Date Recue/Date Received 2020-08-31
determined by fingerprinting techniques (e.g., making use of isozyme markers
and/or molecular
markers such as Single-nucleotide polymorphism (SNP) markers, amplified
fragment length
polymorphism (AFLP) markers, microsatellites, minisatellites, Random Amplified
Polymorphic
DNA (RAPD) markers, restriction fragment length polymorphism (RFLP) markers
and others). A
plant is "closely related" to tomato variety NUN 09247 TOF if its DNA
fingerprint is at least 80%,
90%, 95% or 98% identical to the fingerprint of that variety. In a particular
aspect, AFLP markers
are used for DNA fingerprinting (see, e.g., Vos et al. 1995, Nucleic Acid
Research 23: 4407-4414).
A closely related plant may have a Jaccard's Similarity index of at least
about 0.8, preferably at
least about 0.9, 0.95, 0.98 or more (see, e.g., Sharifova, S., et. al.,
(2013), Journal of Hort.
Research, 21(1):83-89; Ince et al., (2010), Biochem. Genet. 48:83-95;
Parvathaneni et al., (2011),
J. Crop Sci. Biotech, 14 (1): 39-43; Pisanu, et. al., (2004), Acta Hort. 660,
83-89). The disclosure
also provides a plant and a variety obtained or selected by applying these
methods on tomato
variety NUN 09247 TOF. Such a plant may be produced by crossing and/or
selfing, or
alternatively, a plant may simply be identified and selected amongst plants of
said variety, or
progeny of said variety, e.g. by identifying a variant within tomato variety
NUN 09247 TOF or
within progeny of said variety (e.g., produced by selfing) which variant
differs from the variety
described herein in one, two or three of the morphological and/or
physiological characteristics
(e.g., in one, two or three distinguishing characteristics), e.g. those listed
in Tables 1 and 2. In one
aspect, the disclosure provides a tomato plant having a Jaccard's Similarity
index with tomato
variety NUN 09247 TOF of at least 0.8, e.g. at least 0.85, 0.9, 0.95, 0.98 or
even at least 0.99.
[98] In some aspects, the disclosure provides a tomato plant comprising
genomic DNA having
at least 95%, 96%, 97%, 98% or 99% sequence identity compared to the genomic
DNA sequence
of a plant of variety NUN 09247 TOF as deposited under Accession Number NCIMB
43642. In
some aspects, the tomato plant further comprises all or all but 1, 2, or 3 of
the morphological and
physiological characteristics of tomato variety NUN 09247 TOF (e.g., as listed
in Tables 1 and 2).
In other aspects, the tomato plant is a hybrid or other derived from a seed or
plant of variety NUN
09247 TOF. In other aspects, the tomato plant comprises the distinguishing
characteristics of
tomato variety NUN 09247 TOF.
[99] For the purpose of this disclosure, the "sequence identity" of nucleotide
sequences,
expressed as a percentage, refers to the number of positions in the two
optimally aligned sequences
which have identical residues (x100) divided by the number of positions
compared. A gap, i.e., a
29
Date Recue/Date Received 2020-08-31
position in the pairwise alignment where a residue is present in one sequence
but not in the other,
is regarded as a position with non-identical residues. A pairwise global
sequence alignment of two
nucleotide sequences is found by aligning the two sequences over the entire
length according to
the Needleman and Wunsch global alignment algorithm described in Needleman and
Wunsch,
1970, J. Mol. Biol. 48(3):443-53). A full implementation of the Needleman-
Wunsch global
alignment algorithm is found in the needle program in The European Molecular
Biology Open
Software Suite (see, e.g., EMBOSS, Rice et al., Trends in Genetics June 2000,
vol. 16, No. 6. pp.
276-277).
[100] The disclosure also provides methods for determining the identity of
parental lines of
plants described herein, in particular the identity of the female line. US
2015/0126380, relates to
a non-destructive method for analyzing maternal DNA of a seed. In this method,
the DNA is
dislodged from the seed coat surface and can be used to collect information on
the genome of the
maternal parent of the seed. This method for analyzing maternal DNA of a seed
comprises
contacting a seed with a fluid to dislodge DNA from the seed coat surface, and
analyzing the DNA
thus dislodged from the seed coat surface using methods known in the art. The
skilled person is
thus able to determine whether a seed has grown on a plant of variety NUN
09247 TOF or is a
progeny of said variety, because the seed coat of the seed is a maternal
tissue genetically identical
to tomato variety NUN 09247 TOF. In one aspect, the present disclosure relates
to a seed coat
comprising maternal tissue of tomato variety NUN 09247 TOF. In another aspect,
the disclosure
relates to a tomato seed comprising a maternal tissue of tomato variety NUN
09247 TOF. In
another particular aspect, the disclosure provides for a method of identifying
the female parental
line of tomato variety NUN 09247 TOF by analyzing the seed coat of a seed of
that variety. In
another aspect, the disclosure provides for a method of determining whether a
seed is grown on
tomato variety NUN 09247 TOF by analyzing the seed coat or another maternal
tissue of said seed.
[101] By crossing and/or selfing, (one or more) single traits may be
introduced into tomato
variety NUN 09247 TOF (e.g., using backcrossing breeding schemes), while
retaining the
remaining morphological and physiological characteristics of said variety
and/or while retaining
one or more or all distinguishing characteristics. A single trait converted
plant may thereby be
produced. For example, disease resistance genes may be introduced, genes
responsible for one or
more quality traits, yield, etc. Both single genes (e.g., dominant or
recessive) and one or more
Date Recue/Date Received 2020-08-31
QTLs (quantitative trait loci) may be transferred into tomato variety NUN
09247 TOF by breeding
with said variety.
[102] Alternatively, a single trait converted plant or single locus converted
plant of variety NUN
09247 TOF may be produced by (i) genetically transforming or mutating cells of
tomato variety
NUN 09247 TOF; (ii) growing the cells into a plant; and (iii) optionally
selecting a plant that
contains the desired single locus conversion. The skilled person is familiar
with various techniques
for genetically transforming a single locus in a plant cell, or mutating said
cells.
[103] Any pest or disease resistance genes may be introduced into a plant of
tomato variety NUN
09247 TOF, progeny of said variety or into a plant comprising all but 1, 2, or
3 or more of the
morphological and physiological characteristics of tomato variety NUN 09247
TOF, respectively
(e.g., as listed in Tables 1 and 2). Resistance to one or more of the
following diseases or pests may
be introduced into the plant described herein: Colorado potato beetle,
Southern root knot
nematode, Spider mites, Sugarfly beet army worm, Tobacco flea beetle, Tomato
hornworm,
Tomato fruitworm, Whitefly, Bacterial canker, Bacterial soft rot, Bacterial
speck, Bacterial wilt
(Pseudomonas syringae pv. Tomato), Bacterial, Anthracnose (Gloeosporium
pperatum), Brown
rot or corky root (Pyrenochaeta lycopersici), Alternaria, Fusarium wilt (E
oxysporum races),
Gray leaf spot (Stemphylium spp.), Late blight (Phytophthora infestans races),
and Leaf mold
(Cladosporium fulvum races), Nematode (Illeloidogyne spp.), Verticillium Wilt
(Verticillium
dahliae), Ralstonia solanacearum (Rs), Leveillula Taurica (Lt), and/or O/d/um
neolycopersici
(On). Other resistance genes, against pathogenic viruses (e.g., Tomato Mosaic
Virus (ToMV),
Curly TOF Virus, Tomato Mottle Virus, Potato Y Virus, Blotchey Ripening,
Tobacco Etch Virus,
the various Tobacco Mosaic Virus races, Concentric cracking, Tomato Spotted
Wilt Virus
(TSWV), Tomato Yellow Leaf Curl Virus (TYLCV), Gold Fleck, Tomato Torrado
Virus (ToTV)),
fungi, bacteria, nematodes, insects or other pests may also be introduced.
[104] The disclosure also provides a method for developing a tomato plant in a
tomato breeding
program, using a tomato plant described herein, or its parts as a source of
plant breeding material.
Suitable plant breeding techniques are recurrent selection, backcrossing,
pedigree breeding, mass
selection, mutation breeding and/or genetic marker enhanced selection. In one
aspect, the method
comprises crossing tomato variety NUN 09247 TOF or progeny of said variety, or
a plant
comprising all but 1, 2, or 3 or more of the morphological and physiological
characteristics of
31
Date Recue/Date Received 2020-08-31
tomato variety NUN 09247 TOF (e.g., as listed in Tables 1 and 2), with a
different tomato plant,
and wherein one or more offspring of the crossing are subject to one or more
plant breeding
techniques: recurrent selection, backcrossing, pedigree breeding, mass
selection, mutation
breeding and genetic marker enhanced selection (see, e.g., Vidaysky and
Czosnek, (1998)
Phytopathology 88(9): 910-4). For breeding methods in general, see, e.g.,
Principles of Plant
Genetics and Breeding, 2007, George Acquaah, Blackwell Publishing, ISBN-13:
978-1-4051-
3646-4.
[105] The disclosure also provides a tomato plant comprising at least a first
set of the
chromosomes of tomato variety NUN 09247 TOF, a sample of seed of said variety
is deposited
under Accession Number NCIMB 43642; optionally further comprising a single
locus conversion
or a mutation, wherein said plant has essentially all of the morphological and
physiological
characteristics of the plant comprising at least a first set of the
chromosomes of said variety. In
another aspect, this single locus conversion confers a trait, wherein the
trait is yield, storage
properties, color, flavor, male sterility, herbicide tolerance, insect
resistance, pest resistance,
disease resistance, environmental stress tolerance, modified carbohydrate
metabolism, modified
protein metabolism or ripening, or the mutation occurs in any of the following
genes acs2, acs4,
rin, pp2c1, arP, intense, myb12.
[106] In one aspect, a plant of variety NUN 09247 TOF may also be mutated (by
e.g., irradiation,
chemical mutagenesis, heat treatment, etc.) and mutated seeds or plants may be
selected in order
to change one or more characteristics of said variety. Methods such as TILLING
may be applied
to tomato populations in order to identify mutants. Similarly, tomato variety
NUN 09247 TOF
may be transformed and regenerated, whereby one or more chimeric genes are
introduced into the
variety or into a plant comprising all but 1, 2, 3, or more of the
morphological and physiological
characteristics (e.g., as listed in Tables 1 and 2). Transformation can be
carried out using standard
methods, such as Agrobacterium tumefaciens mediated transformation or
biolistics, followed by
selection of the transformed cells and regeneration into plants. A desired
trait (e.g., gene(s)
conferring pest or disease resistance, herbicide, fungicide or insecticide
tolerance, etc.) can be
introduced into tomato variety NUN 09247 TOF, or progeny of said variety, by
transforming said
variety or progeny of said variety with a transgene that confers the desired
trait, wherein the
transformed plant retains all or all but one, two or three of the phenotypic
and/or morphological
32
Date Recue/Date Received 2020-08-31
and/or physiological characteristics of tomato variety NUN 09247 TOF or the
progeny of said
variety and contains the desired trait.
[107] The disclosure also provides a plant or a cell of a plant comprising a
desired trait produced
by mutating a plant of variety NUN 09247 TOF or a cell thereof and selecting a
plant the desired
trait, wherein the mutated plant retains all or all but one of the phenotypic
and morphological
characteristics of said variety, optionally as described for each variety in
in Tables 1 and 2, and
contains the desired trait and wherein a representative sample of seed of
variety NUN 09247 TOF
is deposited under Accession Number NCIMB 43642. In a further aspect, the
desired trait is yield,
storage properties, color, flavor, male sterility, herbicide tolerance, insect
resistance, pest
resistance, disease resistance, environmental stress tolerance, modified
carbohydrate metabolism,
modified protein metabolism or ripening, or the mutation occurs in any of the
following genes
acs2, acs4, rin, pp2c1, ar/9, intense, myb12.
[108] In one aspect, the disclosure provides a method for inducing a mutation
in tomato variety
NUN 09247 TOF comprising:
a) exposing the seed, plant, plant part, or cell of tomato variety NUN
09247 TOF to a
mutagenic compound or to radiation, wherein a representative sample of seed of
said tomato variety is deposited under Accession Number NCIMB 43642;
b) selecting the seed, plant, plant part, or cell of tomato variety NUN 09247
TOF
having a mutation; and
c) optionally growing and/or multiplying the seed, plant, plant part, or
cell of tomato
variety NUN 09247 TOF having the mutation.
[109] The disclosure also provides a plant having one, two, or three
physiological and/or
morphological characteristics which are different from those of tomato variety
NUN 09247 TOF
and which otherwise has all the physiological and morphological
characteristics of said variety,
wherein a representative sample of seed of tomato variety NUN 09247 TOF is
deposited under
Accession Number NCIMB 43642. In particular, variants which differ from tomato
variety NUN
09247 TOF, in none, one, two or three of the characteristics mentioned in
Tables 1 and 2 are
encompassed.
33
Date Recue/Date Received 2020-08-31
[110] A part of the plant of variety NUN 09247 TOF (or of progeny of said
variety or of a plant
having all physiological and/or morphological characteristics but one, two, or
three which are
different from those of said variety) encompasses any cells, tissues, organs
obtainable from the
seedlings or plants, such as but not limited to: a tomato fruit or a part
thereof, a cutting, a hypocotyl,
a cotyledon, seed coat, pollen and the like. Such parts can be stored and/or
processed further. The
disclosure further provides for food or feed products comprising a part of
tomato variety NUN
09247 TOF or a part of progeny of said variety, or a part of a plant having
all but one, two, or three
physiological and/or morphological characteristics of tomato variety NUN 09247
TOF,
comprising one or more of such parts, optionally processed (such as canned,
chopped, cooked,
roasted, in a sauce, in a sandwich, pasted, puréed or concentrated, juiced,
frozen, dried, pickled, or
powdered).
[111] In another aspect, the disclosure provides for a haploid plant and/or a
doubled haploid plant
of tomato variety NUN 09247 TOF, or of a plant having all but one, two, or
three physiological
and/or morphological characteristics of tomato variety NUN 09247 TOF, or
progeny of any of
these, is encompassed herein. Haploid and doubled haploid (DH) plants can, for
example, be
produced by cell or tissue culture and chromosome doubling agents and
regeneration into a whole
plant. DH production chromosome doubling may be induced using known methods,
such as
colchicine treatment or the like. In one aspect, the method comprises inducing
a cell or tissue
culture with a chromosome doubling agent and regenerating the cells or tissues
into a whole plant.
[112] In another aspect, the disclosure comprises a method for making doubled
haploid cells from
haploid cells of tomato variety NUN 09247 TOF comprising doubling cells of
tomato variety NUN
09247 TOF with a doubling agent, such as colchicine treatment (see, e.g.,
Nikolova V,
Niemirowicz-Szczytt K (1996) Acta Soc Bot Pol 65:311-317).
[113] In another aspect, the disclosure provides for haploid plants and/or
doubled haploid plants
derived from tomato variety NUN 09247 TOF that, when combined, make a set of
parents of
tomato variety NUN 09247 TOF. The haploid plant and/or the doubled haploid
plant of variety
NUN 09247 TOF can be used in a method for generating parental lines of tomato
variety NUN
09247 TOF.
[114] Using methods known in the art such as "reverse synthesis of breeding
lines" or "reverse
breeding," it is possible to produce parental lines for a hybrid plant such as
tomato variety NUN
34
Date Recue/Date Received 2020-08-31
09247 TOF. A skilled person can take any individual heterozygous plant (called
a "phenotypically
superior plant" in Example 2 of US 2015/0245570, tomato variety NUN 09247 TOF
is such plant)
and generate a combination of parental lines (reverse breeding parental lines)
that, when crossed,
produce the variety NUN 09247 TOF. It is not necessary that the reverse
breeding parental lines
are identical to the original parental lines. Such new breeding methods are
based on the segregation
of individual alleles in the spores produced by a desired plant and/or in the
progeny derived from
the self-pollination of that desired plant, and on the subsequent
identification of suitable progeny
plants in one generation, or in a limited number of inbred cycles. Such a
method is known from
US 2015/0245570 or from Wijnker et al., Nature Protocols Volume: 9, Pages: 761-
772 (2014)
DOT: doi:10.1038/nprot.2014.049. Thus, the disclosure provides a method for
producing parental
lines for a hybrid organism (e.g., tomato variety NUN 09247 TOF), comprising
in one aspect: a)
defining a set of genetic markers present in a heterozygous form (H) in a
partially heterozygous
starting organism; b) producing doubled haploid lines from spores of the
starting organism; c)
genetically characterizing the doubled haploid lines thus obtained for the
said set of genetic
markers to determine whether they are present in a first homozygous form (A)
or in a second
homozygous form (B); and d) selecting at least one pair of doubled haploid
lines that have
complementary alleles for at least a subset of the genetic markers, wherein
each member of the
pair is suitable as a parental line for the hybrid organism.
[115] In another aspect, the method for producing parental lines of a hybrid
organisms, e.g., of
tomato variety NUN 09247 TOF, which when crossed reconstitute the genome of
tomato variety
NUN 09247 TOF, comprising:
a) defining a set of genetic markers that are present in a heterozygous form
(H) in a
partially heterozygous starting organism;
b) producing at least one further generation from the starting organism by
self-
pollination (e.g., F2 or F3 generation);
c) selecting at least one pair of progeny organisms in which at least
one genetic
marker from the set is present in a complementary homozygous form (B vs. A, or
A vs. B); and
Date Recue/Date Received 2020-08-31
d) optionally repeating steps b) and c) until at least one pair of progeny
organisms
that have complementary alleles for at least a subset of the genetic markers
has
been selected as parental lines for a hybrid.
[116] The disclosure also provides a method for producing parental lines for
hybrid NUN 09247
TOF comprising: genetically characterizing a doubled haploid line from tomato
variety NUN
09247 TOF to determine whether one or more genetic markers are present in a
first homozygous
form or in a second homozygous form in said line, wherein the one or more
genetic markers are
present in a heterozygous form in tomato variety NUN 09247 TOF; and selecting
at least one pair
of doubled haploid lines that have complementary alleles for the one or more
the genetic markers,
wherein each member of the pair is suitable as a parental line for a hybrid
organism, optionally
this method further comprises defining a set of genetic markers present in a
heterozygous form in
tomato variety NUN 09247 TOF; and producing doubled haploid lines from tomato
variety NUN
09247 TOF. Doubled haploid lines generated as described herein can be used in
such a method.
[117] Thus, in one aspect, the disclosure relates to a method of producing a
combination of
parental lines of a plant of variety NUN 09247 TOF comprising making doubled
haploid cells
from haploid cells or seed of that plant; and optionally crossing these
parental lines to produce and
collecting seeds. In another aspect, the disclosure relates to a combination
of parental lines
produced by this method. In still another aspect, the combination of parental
lines can be used to
produce a seed or plant of variety NUN 09247 TOF when these parental lines are
crossed. In still
another aspect, the disclosure relates to a combination of parental lines from
which a seed or plant
having all physiological and/or morphological characteristics of tomato
variety NUN 09247 TOF
(when the characteristics are determined at the 5% significance level for
plants grown under the
same conditions).
[118] In another aspect, the disclosure provides a method of introducing a
single locus
conversion, single trait conversion, or a desired trait into tomato variety
NUN 09247 TOF
comprising:
a) obtaining a combination of a parental lines of tomato variety NUN 09247
TOF,
optionally through reverse synthesis of breeding lines,
b) introducing a single locus conversion, single trait conversion, or a
desired trait in at
least one of the parents of step a); and
36
Date Recue/Date Received 2020-08-31
c) crossing the converted parent with the other parent of step a) to obtain
seed of tomato
variety NUN 09247 TOF.
[119] A combination of a male and a female parental line of tomato variety NUN
09247 TOF can
be generated by methods described herein, for example, through reverse
synthesis of breeding
lines.
[120] In another aspect, the disclosure provides a method of introducing a
single locus
conversion, single trait conversion, or a desired trait into tomato variety
NUN 09247 TOF,
comprising introducing a single locus conversion, single trait conversion, or
a desired trait in at
least one of the parents of tomato variety NUN 09247 TOF; and crossing the
converted parent with
the other parent of tomato variety NUN 09247 TOF to obtain seed of tomato
variety NUN 09247
TOF.
[121] In another aspect, the step of introducing a single locus conversion,
single trait conversion,
or a desired trait in at least one of the parents comprises:
a) obtaining a cell or tissue culture of cells of the parental line of
tomato variety NUN
09247 TOF;
b) genetically transforming or mutating said cells;
c) growing the cells into a plant; and
d) optionally selecting plants that contain the single locus conversion, the
single trait
conversion, or the desired trait.
[122] In another method, the step of introducing a single locus conversion,
single trait conversion,
or a desired trait in at least one of the parents comprises genetically
transforming or mutating cells
the parental line of tomato variety NUN 09247 TOF; growing the cells into a
plant; and optionally
selecting plants that contain the single locus conversion, the single trait
conversion, or the desired
trait.
[123] In another aspect, the step of introducing a single locus conversion,
single trait conversion,
or a desired trait in at least one of the parents comprises:
37
Date Recue/Date Received 2020-08-31
a) crossing the parental line of tomato variety NUN 09247 TOF with a second
tomato
plant comprising the single locus conversion, the single trait conversion, or
the desired
trait;
b) selecting F 1progeny plants that contain the single locus conversion,
the single trait
conversion, or the desired trait;
c) crossing said selected progeny plants of step b) with the parental line
of step a) to
produce a backcross progeny plant;
d) selecting backcross progeny plants comprising the single locus
conversion, the single
trait conversion or the desired trait and otherwise all or all but one, two or
three of the
morphological and physiological characteristics the parental line of step a)
to produce
selected backcross progeny plants; and
e) optionally repeating steps c) and d) one or more times in succession to
produce
selected second, third or fourth or higher backcross progeny plants comprising
the
single locus conversion, the single trait conversion or the desired trait and
otherwise
all or all but one, two or three of the morphological and physiological
characteristics
the parental line of step a) to produce selected backcross progeny plants,
when grown
in the same environmental conditions.
The disclosure further relates to plants obtained by this method.
[124] In any of the above methods, where the single locus conversion, single
trait conversion, or
a desired trait concerns a trait, the trait may be yield or pest resistance or
disease resistance. In one
aspect, the trait is disease resistance and the resistance are conferred to
Colorado potato beetle,
Southern root knot nematode, Spider mites, Sugarfly beet army worm, Tobacco
flea beetle,
Tomato hornworm, Tomato fruitworm, Whitefly, Bacterial canker, Bacterial soft
rot, Bacterial
speck, Bacterial wilt (Pseudomonas syringae pv. Tomato), Bacterial,
Anthracnose (Gloeosporium
pperatum), Brown rot or corky root (Pyrenochaeta lycopersici), Alternaria,
Fusarium wilt (F.
oxysporum races), Gray leaf spot (Stemphylium spp.), Late blight (Phytophthora
infestans races),
and Leaf mold (Cladosporium fulvum races), Nematode (Aleloidogyne spp.),
Verticillium Wilt
(Verticillium dahliae), Ralstonia solanacearum (Rs), Leveillula Taurica (Lt),
and/or O/d/um
neolycopersici (On). Other resistance genes, against pathogenic viruses (e.g.,
Tomato Mosaic
38
Date Recue/Date Received 2020-08-31
Virus (ToMV), Curly TOF Virus, Tomato Mottle Virus, Potato Y Virus, Blotchey
Ripening,
Tobacco Etch Virus, the various Tobacco Mosaic Virus races, Concentric
cracking, Tomato
Spotted Wilt Virus (TSWV), Tomato Yellow Leaf Curl Virus (TYLCV), Gold Fleck,
Tomato
Torrado Virus (ToTV)), fungi, bacteria, nematodes, insects or other pests may
also be introduced.
[125] The disclosure also provides a combination of parental lines which, when
crossed, produce
a seed or plant having all physiological and/or morphological characteristics
of tomato variety
NUN 09247 TOF but one, two or three which are different (when grown under the
same
environmental conditions), as well as a seed or plant having all physiological
and/or morphological
characteristics of tomato variety NUN 09247 TOF but one, two, or three which
are different (when
the numerical characteristics are determined at the 5% significance level and
determined by type
or degree for non-numerical characteristics for plants grown under the same
environmental
conditions).
[126] Also provided is a plant part obtainable from variety NUN 09247 TOF or
from progeny of
said variety or from a plant having all but one, two or three tomato variety
09247 TOF or from a
vegetatively propagated plant of variety NUN 09247 TOF (or from its progeny or
from a plant
having all or all but one, two or three physiological and/or morphological
characteristics which are
different from those of tomato variety NUN 09247 TOF), wherein the plant part
is a fruit, a
harvested fruit, a part of a fruit, a leaf, a part of a leaf, pollen, an
ovule, a cell, a petiole, a shoot or
a part thereof, a stem or a part thereof, a root or a part thereof, a root
tip, a cutting, a seed, a part
of a seed, seed-coat or another maternal tissue which is part of a seed grown
on tomato variety
NUN 09247 TOF, or a hypocotyl, a cotyledon, a scion, a stock, a rootstock, a
pistil, an anther, or
a flower or a part thereof
[127] The disclosure also provides a combination of parental lines which, when
crossed, produce
a seed or plant having all physiological and/or morphological characteristics
of tomato variety
NUN 09247 TOF one, two or three which are different (when grown under the same
environmental
conditions), as well as a seed or plant having all physiological and/or
morphological characteristics
of tomato variety NUN 09220 TOF, but one, two or three which are different
(when the
characteristics are determined at the 5% significance level for plants grown
under the same
conditions).
39
Date Recue/Date Received 2020-08-31
[128] In another aspect, the disclosure provides a method of determining the
genotype of a plant
described herein comprising detecting in the genome (e.g., a sample of nucleic
acids) of the plant
at least a first polymorphism or an allele. The skilled person is familiar
with many suitable methods
of genotyping, detecting a polymorphism or detecting an allele including SNP
(Single Nucleotide
Polymorphism) genotyping, restriction fragment length polymorphism
identification (RFLP) of
genomic DNA, random amplified polymorphic detection (RAPD) of genomic DNA,
amplified
fragment length polymorphism detection (AFLP), polymerase chain reaction
(PCR), DNA
sequencing, allele specific oligonucleotide (ASO) probes, and hybridization to
DNA microarrays
or beads. Alternatively, the entire genome could be sequenced. The method may,
in certain
embodiments, comprise detecting a plurality of polymorphisms in the genome of
the plant, for
example by obtaining a sample of nucleic acid from a plant and detecting in
said nucleic acids a
plurality of polymorphisms. The method may further comprise storing the
results of the step of
detecting the plurality of polymorphisms on a computer readable medium.
[129] The disclosure also provides for a food or feed product comprising or
consisting of a plant
part described herein. Preferably, the plant part is a tomato fruit or part
thereof and/or an extract
from a fruit or another plant part described herein. The food or feed product
may be fresh or
processed, e.g., dried, grinded, powdered, pickled, chopped, cooked, roasted,
in a sauce, in a
sandwich, pasted, puréed or concentrated, juiced, pickled, canned, steamed,
boiled, fried, blanched
and/or frozen, etc.
[130] Marketable tomato fruits are generally sorted by size and quality after
harvest.
Alternatively, the tomato fruits can be sorted by expected shelf life, pH, or
Brix.
[131] Tomato variety NUN 09247 TOF may also be grown for use as rootstocks
(stocks) or
scions. Typically, different types of tomatoes are grafted to enhance disease
resistance, which is
usually conferred by the rootstock, while retaining the horticultural
qualities usually conferred by
the scion. It is not uncommon for grafting to occur between cultivated tomato
varieties and related
tomato species. Methods of grafting and vegetative propagation are well-known
in the art.
[132] In another aspect, the disclosure provides to a plant comprising a
rootstock or scion of
tomato variety NUN 09247 TOF.
[133] References:
Date Recue/Date Received 2020-08-31
UPOV, Guidelines for the Conduct of Tests for Distinctness, Uniformity and
Stability, TG/13/11,
March 2013.
US Department of Agriculture, Agricultural Marketing Service, Objective
Description of Variety
Tomato (Solanum lycopersicum or Lycopersicon esculentum Mill), June 2015.
Acquaah, Principles of Plant Genetics and Breeding, 2007, Blackwell
Publishing, ISBN-13: 978-
1-4051-3646-4 .
Bhatia, P., et al., Tissue Culture Studies of Tomato (Lycopersicum
esculentum), Plant Cell, Tissue
and Organ Culture, 2004, vol. 78, pp. 1-21.
Ince, A. G., et al., Genetic Relationship Within and Between Capsicum Species,
Biochem Genet,
2010, vol. 48, pp. 83-95.
Hartz, et. al., "Processing Tomato Production in California," University of
California Division of
Agriculture and Natural Resources, 2008, Publication 7228, pp. 1-5.
Needleman, S.B., et. al., A General Method Applicable to the Search for
Similarities in the Amino
Acid Sequence of Two Proteins, Journal of Molecular Biology, 1970, vol. 48(3),
pp. 443-53.
Nikolova, V., et. al., Diploidization of Cucumber (Cucumis sativus L.)
Haploids by Colchini
Treatment,Acta Societas Botanicorum Poloniae, 1996, vol. 65, pp. 311-317.
Pisanu, A. B., et. al., "Yield and Biometric Characteristics of 9 Clones
Selected from the
Population of "Spinoso sardo "Artichokes, Acta Hort., 2004, ISHS 660, pp. 83-
89.
Rice et al., EMBOSS: The European Molecular Biology Open Software Suite,
Trends in Genetics,
2000, vol. 16, Issue 6. pp. 276-277.
Sharifova, S., et. al., "Assessment of Genetic Diversity in Cultivated Tomato
(Solanum
lycopersicum L.) Genotypes Using RAPD Primers", Journal of Horticultural
Research, 2013, vol.
21, no. 1, pp. 83-89.
Strange, et. al., "Fresh-Market Tomato Production in California," University
of California
Division of Agriculture and Natural Resources, 2000, Publication 8017, pp. 1-
8.
Vidaysky F, Czosnek H., 1998, Tomato Breeding Lines resistant and tolerant to
tomato yellow
leaf curl virus issued from Lycopersicum hirsutum, Phytopathology,
Sep;88(9):910-4.
41
Date Recue/Date Received 2020-08-31
Vos, P., et al., AFLP: A New Technique for DNA Fingerprinting 1995, Nucleic
Acids Research,
1995, vol. 23, No. 21, pp. 4407-4414.
Wijnker, E., et al., Hybrid Recreation by Reverse breeding in Arabidopsis
thaliana, Nature
Protocols, 2014, vol. 9, pp. 761-772.
US2008/0222949
US9125353
US2002/0010953
US6060648
EP1057401
EP1428425
US2008/0222949
US2015/0126380
US2015/0245570
42
Date Recue/Date Received 2020-08-31
Development of Tomato Variety NUN 09247 TOF
[134] The hybrid variety NUN 09247 TOF was developed from a male and female
proprietary
inbred line of Nunhems, selected mainly for its fruit number and good flower.
The female and
male parents were crossed to produce hybrid (F1) seeds of tomato variety NUN
09247 TOF. The
seeds of tomato variety NUN 09247 TOF can be grown to produce hybrid plants
and parts thereof
(e.g., tomato fruit). The hybrid variety NUN 09247 TOF can be propagated by
seeds or
vegetatively.
[135] The hybrid variety is uniform and genetically stable. This has been
established through
evaluation of horticultural characteristics. Several hybrid seed production
events resulted in no
observable deviation in genetic stability. Coupled with the confirmation of
genetic stability of the
female and male parents the Applicant has concluded that tomato variety NUN
09247 TOF is
uniform and stable.
Deposit Information
[136] A total of 2500 seeds of the hybrid variety NUN 09247 TOF will be
deposited according
to the Budapest Treaty by Nunhems B.V. on July 22, 2020, at the NCIMB Ltd.,
Ferguson Building,
Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, United Kingdom (NCIMB). The
deposit will
be assigned NCIMB number 43642. A statement indicating the viability of the
sample will be
provided. A deposit of tomato variety NUN 09247 TOF and of the male and female
parent line is
also maintained at Nunhems B.V. The seed lot number for Tomato variety NUN
09247 TOF is
28438601002.
[137] The deposit will be maintained in NCIMB for a period of 30 years, or 5
years after the most
recent request, or for the enforceable life of the patent whichever is longer
and will be replaced if
it ever becomes nonviable during that period. Access to the deposit will be
available during the
pendency of this application to persons determined by the Director of the U.S.
Patent Office to be
entitled thereto upon request. All restrictions imposed by the depositor on
the availability to the
public of the deposited material will be irrevocably removed upon the granting
of the patent.
Applicant does not waive any rights granted under this patent on this
application or under the Plant
Variety Protection Act (7 U.S.C. 2321 et seq.). Accordingly, the
requirements of 37 CFR 1.801-
1.809 will be satisfied.
43
Date Recue/Date Received 2020-08-31
Characteristics of Tomato Variety NUN 09247 TOF
[138] The most similar variety to NUN 09247 TOF is Sweetelle, a variety from
Syngenta.
[139] In Tables 1 and 2, a comparison between tomato variety NUN 09247 TOF and
the
Reference Variety is shown based on a trial in the Netherlands during the
trial season 2017.
[140] Two replications of 12 plants of each variety, from which at least 10
plants or plant parts
were randomly selected and were used to measure characteristics. For numerical
characteristics
averages were calculated. For non-numerical characteristics, the type/degree
were determined.
[141] In another aspect, the disclosure provides a plant having the
physiological and
morphological characteristics of tomato variety NUN 09247 TOF as presented in
Tables 1 and 2.
Table 1. Characteristics of Tomato Variety NUN 09247 TOF and the Reference
Variety
Characteristics Application Variety Reference Variety
(NUN 09247 TOF) (Sweetelle)
Seedling:
Anthocyanin coloration of hypocotyl: Present Present
1= Absent; 2= Present
Plant:
Growth type: 1= Determinate; 2= Indeterminate Indeterminate
Indeterminate; 3= Semi-determinant;
4= Semi indeterminant
Plant height: 1= Very short; 3= Short; Very long Medium to
long
5= Medium; 7= Long; 9= Very long
Stem:
Anthocyanin coloration: 1= Absent or Absent or very weak
Weak
very weak; 3= Weak; 5= Medium; 7=
Strong; 9= Very strong
Length of internode: 3= Short; 5= Medium Medium
Medium; 7= Long
Leaf
Attitude: 1= Erect; 2= Semi-erect; 5= Horizontal Horizontal
to semi-
Horizontal; 7= Semi-drooping; 9= drooping
Drooping
Length: 3= Short; 5= Medium; 7= Medium Long
Long
Width: 3= Narrow; 5= Medium; 7= Medium Medium
Broad
Type of blade: 1= Pinnate; 2= Bipinnate Bipinnate
Bipinnate
44
Date Recue/Date Received 2020-08-31
Characteristics Application Variety Reference Variety
(NUN 09247 TOF) (Sweetelle)
Size of leaflets (in middle of leaf): 1= Small Medium to large
Very small; 3= Small; 5= Medium; 7=
Large; 9= Very large
Intensity of green color: 1= Very light; Dark Dark
3= Light; 5= Medium; 7= Dark; 9=
Very dark
Glossiness: 3= Weak; 5= Medium; 7= Medium Medium
Strong
Blistering: 3= Weak; 5= Medium; 7= Medium Weak to medium
Strong
Attitude of petiole of leaflet in relation Horizontal Semi-
erect
to main axis: 3= Semi-erect; 5=
Horizontal; 7= Semi-drooping
Flower:
Inflorescence type: 1= Mainly Equally uniparous and Mainly multiparous
uniparous; 2= Equally uniparous and multiparous
multiparous; 3= Mainly multiparous
Color: 1= Yellow; 2= Orange Yellow Yellow
Pubescence of style: 1= Absent or Present Present
very scarce; 9= Present
Peduncle:
Abscission layer: 1= Absent; 2= Present Present
Present
Pedicel length: 3= Short; 5= Medium; Medium Medium
7= Long
Fruit:
Green shoulder (before maturity): 1= Absent Present
Absent; 9= Present
Extent of green shoulder (before No green shoulder Medium to
large
maturity): 1= Very small; 3= Small;
5= Medium; 7= Large
Intensity of green color of shoulder No green shoulder Dark
(before maturity): 3= Light; 5=
Medium; 7= Dark
Intensity of green color excluding No green shoulder Light to
medium
shoulder (before maturity): 1= Very
light; 3= Light; 5= Medium; 7= Dark;
9= Very dark
Green stripes (before maturity): 1= Absent Absent
Absent; 9= Present
Size: 1= Very small; 2= Very small to Very small to small Very
small to small
small; 3= Small; 4= Small to medium;
5= Medium; 6= Medium to large; 7=
Date Recue/Date Received 2020-08-31
Characteristics Application Variety Reference
Variety
(NUN 09247 TOF) (Sweetelle)
Large; 8= Large to very large; 9=
Very large
Weight of ripened fruit (grams): 10 g 12g
Shape in longitudinal section: 1= Elliptic Cordate
Flattened; 2= Oblate; 3= Circular; 4=
Oblong; 5= Cylindrical; 6= Elliptic;
7= Cordate; 8= Ovate; 9= Obovate;
10= Pyriform; 11= Obcordate
Ribbing at peduncle end: 1= Absent or Weak Very weak
to weak
very weak; 3= Weak; 5= Medium; 7=
Strong; 9= Very strong
Depression at peduncle end: 1= Absent or very weak Very weak
to weak
Absent or very weak; 3= Weak; 5=
Medium; 7= Strong; 9= Very strong
Size of peduncle scar: 1= Very small; Very small Very
small
3= Small; 5= Medium; 7= Large; 9=
Very large
Size of blossom scar: = Very small; 3= Very small Very
small
Small; 5= Medium; 7= Large; 9= Very
large
Shape at blossom end: 1= Indented; 2= Flat Indented to flat
Indented to flat; 3= Flat; 4= Flat to
pointed; 5= Pointed
Size of core in cross section; 1= Very Very small
Very small to small
small; 3= Small; 5= Medium; 7=
Large; 9= Very thick
Thickness of pericarp: 1= Very thin; Medium Very thin to thin
3= Thin; 5- Medium; 7= Thick; 9=
Very thick
Number of locules: 1= Only two; 2= Only two Only two
Two or three; 3= Three or four; 4=
Four, five, or six; 5= More than six
Color of immature fruit: Green Green
Color at maturity: 1= Cream; 2= Red Red
Yellow; 3= Orange; 4= Pink; 5= Red;
6= Brown; 7= Green
Color of flesh (at maturity): 1= Cream; Red Red
2= Yellow; 3= Orange; 4= Pink; 5=
Red; 6= Brown; 7= Green
Glossiness of skin: 1= Weak; 2= Medium Medium
Medium; 3= Strong
Color of epidermis: 1= Colorless; 2= Colorless Colorless
Yellow
46
Date Recue/Date Received 2020-08-31
Characteristics Application Variety Reference
Variety
(NUN 09247 TOF) (Sweetelle)
Firmness: 1= Very soft; 3= Soft; 5= Firm Firm
Medium; 7= Firm; 9= Very firm
Shelf life: 1= Very short; 3= Short; 5= Very long,
24 days Very long, 24 days
Medium; 7= Long/ 9= Very long
Maturity:
Time of flowering (50% of the plants Medium Early to
medium
with at least one open flower from
seed sowing): 3= Early; 5= Medium;
7= Late
Time of maturity: 1= Very early; 3= Very early
to early Very early to early
Early; 5= Medium; 7= Late; 9= Very
late
Table 2. Disease Resistances of Tomato Variety NUN 09247 TOF and the Reference
Variety
Resistances Application Variety Reference
Variety
(NUN 09247 TOF) (Sweetelle)
Meloidoygne incognita (Mi) Not tested Resistant
1= Susceptible; 2= Intermediate
Resistant; 3= Resistant
Verticillium dahliae Race 0 Absent Absent
1= Absent; 9= Present
Fusarium oxysporum f. sp. Absent Present
Lycopersici Race 0
1= Absent; 9= Present
Fusarium oxysporum f. sp. Absent Absent
Lycopersici Race 1
1= Absent; 9= Present
Fusarium oxysporum f. sp. Absent Not observed
Lycopersici Race 2
1= Absent; 9= Present
Fusarium oxysporum f. sp. radicis Absent Absent
lycopersici
1= Absent; 9= Present
Fulvia fulva Race 0 Not tested Not tested
0= not tested; 1= Absent; 9= Present
Fulvia fulva Group A Present Present
1= Absent; 9= Present
Fulvia fulva Group B Present Present
1= Absent; 9= Present
Fulvia fulva Group C Present Present
1= Absent; 9= Present
47
Date Recue/Date Received 2020-08-31
Resistances Application Variety Reference
Variety
(NUN 09247 TOF ) (Sweetelle)
Fulvia fulva Group D Present Present
1= Absent; 9= Present
Fulvia fulva Group E Present Present
1= Absent; 9= Present
Tomato Mosaic Virus (ToMV) Strain Present Present
0
1= Absent; 9= Present
Tomato Mosaic Virus (ToMV) Strain Present Present
1
1= Absent; 9= Present
Tomato Mosaic Virus (ToMV) Strain Present Present
2
1= Absent; 9= Present
Tomato Mosaic Virus (ToMV) Strain Present Present
1-2
1= Absent; 9= Present
Phytophthora infestans Absent Absent
1= Absent; 9= Present
Pyrenochaeta lycopersici Not tested Not tested
0= not tested; 1= Absent; 9= Present
Stemphylium spp. Not tested Not tested
0= not tested; 1= Absent; 9= Present
Pseudomonas syringae pv tomato Not tested Not tested
0= not tested; 1= Absent; 9= Present
Ralstonia solanacearum Race 1 Not tested Not tested
0= not tested; 1= Absent; 9= Present
Tomato Yellow Leaf Curl Virus Absent Absent
(TYLCV)
1= Absent; 9= Present
Tomato Spotted Wilt Virus Race 0 Absent Absent
1= Absent; 9= Present
Leveillula Taurica Not tested Not tested
0= not tested; 1= Absent; 9= Present
O/d/um neolypersici Not tested Not tested
0= not tested; 1= Absent; 9= Present
Torrado Virus Not tested Not tested
0= not tested; 1= Absent; 9= Present
48
Date Recue/Date Received 2020-08-31
Table 3. Distinguishing Characteristics between Tomato Variety NUN 09247 TOF
and the
Reference Variety
Characteristics Application Variety Reference Variety
(NUN 09247 TOF) (Sweetelle)
Plant height:
Plant height: 1= Very short; 3= Short; Very long Medium to
long
5= Medium; 7= Long; 9= Very long
Leaf
Attitude: 1= Erect; 2= Semi-erect; 5= Horizontal Horizontal
to semi-
Horizontal; 7= Semi-drooping; 9= drooping
Drooping
Length: 3= Short; 5= Medium; 7= Medium Long
Long
Width: 3= Narrow; 5= Medium; 7= Broad Medium
Broad
Size of leaflets (in middle of leaf): 1= Small Medium to
large
Very small; 3= Small; 5= Medium; 7=
Large; 9= Very large
Attitude of petiole of leaflet in relation Horizontal Semi-
erect
to main axis: 3= Semi-erect; 5=
Horizontal; 7= Semi-drooping
Flower:
Inflorescence type: 1= Mainly Equally uniparous and Mainly multiparous
uniparous; 2= Equally uniparous and multiparous
multiparous; 3= Mainly multiparous
Fruit:
Green shoulder (before maturity): 1= Absent Present
Absent; 9= Present
Extent of green shoulder (before No green shoulder Medium to large
maturity): 1= Very small; 3= Small;
5= Medium; 7= Large
Intensity of green color of shoulder No green shoulder
Dark
(before maturity): 3= Light; 5=
Medium; 7= Dark
Intensity of green color excluding No green shoulder
Light to medium
shoulder (before maturity): 1= Very
light; 3= Light; 5= Medium; 7= Dark;
9= Very dark
Weight of ripened fruit (grams): 10 g 12g
Shape in longitudinal section: 1= Elliptic Cordate
Flattened; 2= Oblate; 3= Circular; 4=
Oblong; 5= Cylindrical; 6= Elliptic;
7= Cordate; 8= Ovate; 9= Obovate;
10= Pyriform; 11= Obcordate
49
Date Recue/Date Received 2020-08-31
Characteristics Application Variety Reference
Variety
(NUN 09247 TOF) (Sweetelle)
Shape at blossom end: 1= Indented; 2= Flat Indented to
flat
Indented to flat; 3= Flat; 4= Flat to
pointed; 5= Pointed
Thickness of pericarp: 1= Very thin; Medium Very thin to
thin
3= Thin; 5- Medium; 7= Thick; 9=
Very thick
Date Recue/Date Received 2020-08-31
