Note : Les descriptions sont présentées dans la langue officielle dans laquelle elles ont été soumises.
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PERFORMANCE PET FOOD PRODUCT
The present invention relates to a foodstuff comprising feather hydrolysate
for use in
improving performance during exercise and/or improving recovery after exercise
in a dog.
It also relates to a method of improving the performance in a dog during
exercise and/or
improving recovery after exercise, the method comprising administering to a
dog a
foodstuff which comprises feather hydrolysate.
Background
Dogs are commonly selected and trained to assist and/or entertain their
owners. Working
dogs are trained to perform tasks. Such tasks may be intense exercises
performed in
short durations (resistance exercise), as well as prolonged exercises which
require
endurance. Working dogs, such as police or army dogs, include dogs which
perform
rescue, search, herding or hunting. Sporting dogs are selected for their
ability to perform
at high levels of intensity. Sporting dogs, include dogs which race in tracks
or perform
agility tests.
Such working or competing dogs become tired, loose their energy and stamina
and are
unable to perform the next task and/or exercise.
Most commonly, high in carbohydrates products are used as the main source of
fuel in
energy foods for dogs performing short intense exercises.
There is a continuing need to improve performance, enhance stamina and hasten
the
recovery after exercise for working dogs or competing dogs to maintain a high
performance level for each potential new intervention or competition run that
may occur
within the same day.
The present invention provides a composition that addresses this need.
The first aspect of this invention relates to a foodstuff for use in improving
performance
during exercise and/or improving recovery after exercise in a dog.
In particular, the foodstuff can be used for enhancing endurance and/or
prolonging the
stamina of the dog while exercising.
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Improving performance in a dog during exercise can be enhancing the dog's
performance
during the exercise by allowing the dog to continue the exercise without
fatigue. Improving
the recovery period of a dog after an exercise can be reducing the dog's
fatigue.
Enhancing endurance and prolonging stamina of a dog during exercising includes
sustaining performance or boosting the dog's energy to perform tasks during
exercise in
particular successive exercise. Successive exercise can be repetitive exercise
of short
duration, optionally including maximum intensity exercises, repetitive long
duration
exercises or mixed endurance and resistance exercises.
Improving performance and/or improving recovery, includes reducing tissue and
cellular
stresses in the dog's body, for example reducing muscle damage, reducing
inflammation,
reducing oxidative stress and/or reducing body temperature.
It is a major interest for working dogs (search and rescue, army, etc.) to
recover quickly in
order to be used quickly on other missions. Some sports, including agility,
contain several
trials within a day. The use of the present invention after each session aids
the working
dog to maintain their performance level by improving recovery after each
exercise.
A first aspect of the invention provides a foodstuff comprising feather
hydrolysate for use
in improving performance during exercise and/or improving recovery after
exercise in a
dog.
The foodstuff of the first aspect of the present invention has shown to be
effective in
improving the performance during exercise, including successive exercise
and/or
improving the recovery after exercise in a dog. The composition has been
demonstrated
to provide, inter alia, one or more of the following:
- reducing muscle damage,
- reducing inflammation,
- reducing oxidative stress and/or
- reducing body temperature.
All of the above contribute in improving performance during exercise and/or
improving the
recovery after exercise in a dog.
The foodstuff comprises feather hydrolysate.
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Feather hydrolysate is the product produced from hydrolysing feathers, in
particular
poultry feathers. Feather hydrolysate usually contains at least 75% crude
protein (total
amino acid content) of which at least 70% are free amino acids.
Poultry industries produce a large amount of feather wastage. Feathers have a
high
source of keratin protein, in particular 8-keratins that are composed of
protein strands
hydrogen-bonded into 8-pleated sheets to produce tough structures. However,
raw
feathers are insoluble and have a low digestibility, thus the feathers are
hydrolysed in
order for the protein to be digestible.
The feathers (or products containing feathers) may have been hydrolysed under
pressure,
hydrolysed with elevated levels of heat, hydrolysed by using acid hydrolysis
including
using a preconditioning agent such as enzymes before the hydrolysing process
begins or
any combination thereof. Different processing conditions such as time of
hydrolysis,
pressure, temperature and moisture can affect its digestibility, solubility
and bioavailability
of the resulting feather hydrolysate. The resulting feather hydrolysate has
specific
characteristics due to the high level of hydrolysation required; in particular
a high source
of total amino acid (>80%), at least 70% free amino acid, 95% of molecular
weight (<
1000 Dalton (1kDa)).
Feather hydrolysate includes any protein hydrolysate that has a component
which is
made from feathers, in particular poultry feathers. Feather hydrolysate,
includes feather
meal.
Preferably, feather hydrolysate is a source of low molecular weight amino
acids and
L- oligopeptides, for example protamine manufactured by BCF.
Feather hydrolysate can be in the form of a powder, gel or liquid. Feather
hydrolysate can
be mixed with a liquid to form a paste. Preferably, feather hydrolysate is in
the form of a
powder.
Typically, the profile of feather hydrolysate is set out in table 1 below:
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Table 1
Feather hydrolysate profile % on dry matter
Taurine 0.01
Hydroxyproline 0.23
Aspartic Acid 5.33
Threonine 3.70
Serine 7.88
Glutamic Acid 8.13
Proline 8.41
Lanthionine 1.65
Glycine 6.25
Alanine 3.57
Cysteine 4.99
Valine 6.28
Methionine 0.57
lsoleucine 3.79
Leucine 6.59
Tyrosine 2.33
Phenylalanine 3.97
Hydroxylysine 0.01
Histidine 0.61
Ornithine 0.30
Lysine 1.79
Arginine 5.68
Tryptophan 0.47
Moisture 6
Ash 7
Protein 83
Fibre 0.31
The foodstuff may further comprise hydrolysed carbohydrate. Hydrolysed
carbohydrate is
any carbohydrate that is hydrolysed, also called a fast sugar.
In particular, hydrolysed carbohydrate can be one or more of any simple sugar,
maltose,
dextrose, fructose, maltodextrin or a combination thereof.
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Preferably, the hydrolysed carbohydrate is maltodextrin or dextrose or
combination
thereof.
In particular, the hydrolysed carbohydrate is highly soluble and dispersible
in cold water
(temperature below 20 C).
Maltodextrin is an oligosaccharide which is obtained from hydrolysing starch.
Maltodextrin
is a polysaccharide (complex) carbohydrate, which consists of repeating units
of sugars
such as glucose or dextrose.
Dextrose is a simple carbohydrate derived from starch but also naturally found
in foods
such as fruits and honey. Dextrose has the same molecular form as glucose.
However,
dextrose is the biologically active form.
Digestible carbohydrates are hydrolysed carbohydrates. Hydrolysed
carbohydrates are
easily absorbed by the body and thus function rapidly to restore glycogen in
muscle after
exercise.
The foodstuff of the invention is any composition which a dog may consume as
part of its
diet.
The foodstuff of the invention may be or may be used in combination with a
complete and
balanced food which provides all the recommended vitamins and minerals for the
dog in
questions, for example, as described in National Research Council, 1985,
Nutritional
Requirements for Dogs, National Academy Press, Washington DC (ISBN:0-309-03496-
5);
or Association of American Feed Control Officials, Official Publication 1996.
It can be any foodstuff, such as dry product, semi moist product, wet food
product or a
liquid and includes food supplement, a snack or a treat.
The foodstuff according to the present invention encompasses any product which
a pet
consumes in its diet. Thus, the invention covers standard food products
including liquids,
as well as pet food snacks (for example, snack bars, pet chew, crunchy treat,
cereal bars,
snacks, biscuits and sweet products) and supplements.
The foodstuff is preferably a commercial pet food product. Such a product is
preferably
sold as a product for feeding to a dog.
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A typical pet foodstuff contains about 20-30% crude protein and about 10-20%
fat, the
remainder being carbohydrate, including dietary fibre and ash. A typical wet
or moist
product contains (on a dry matter basis) about 40% fat, 50% protein and the
remainder
being fibre and ash. The foodstuff of the invention may be a dry product (with
approximately 5 to approximately 15% moisture), a semi-moist product (with
approximately 15 to approximately 70% moisture) or a wet product (with
approximately 70
to approximately 90% moisture).
The remaining components of the foodstuff are not essential to the invention
and typical
standard products can be included. The combined ingredients of the foodstuff
according
to the invention can provide all of the recommended vitamins and minerals for
the
particular animal in question (a complete and balanced food).
The foodstuff can be provided as a food supplement. The food supplement can be
a
powder, sauce, topping, biscuit, kibble, pocket or tablet that can be
administered with or
without an additional foodstuff. Where the food supplement is administered
with an
additional foodstuff, the food supplement can be administered sequentially
simultaneously
or separately. The food supplement may be mixed with the foodstuff, sprinkled
over the
foodstuff or served separately. Alternatively, the food supplement can be
added to a
liquid provided for drinking such as water or milk.
The foodstuff is preferably a cooked product. It may incorporate meat or
animal derived
material (such as beef, chicken, turkey, lamb, fish, blood plasma, marrow bone
etc. or one
or more thereof). The product alternatively may be meat free (preferably
including a meat
substitute such as soya, maize gluten or a soya product) in order to provide a
protein
source. The foodstuff may contain additional protein sources such as soya
protein
concentrate, milk proteins, gluten etc. The foodstuff may also contain a
starch source
such as one or more grains (e.g. wheat, corn, rice, oats, barley etc.), or may
be starch
free.
Alternatively the foodstuff of the invention may be added, mixed, sprinkled
onto a
complete balanced diet. The diet can be dry, semi moist or wet.
In particular, the foodstuff of the invention may be a powder with a liquid or
is a gel
formulation. The liquid may be any liquid that a dog consumes. The amount of
liquid
used to dilute the mixture is dependent on the dog's palatability when
consuming and can
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be any amount required.
The foodstuff may be a supplement. The supplement may be a liquid or in
particular a
powder. The supplement may be added to any other foodstuff, for example a
liquid
supplement may be added to any food. A powder supplement may be sprinkled on a
dry,
wet or semi-moist food before eating.
The foodstuff may be a composite product containing a part which is a dry food
and a part
which is either semi-moist or wet. In such a product, the feather hydrolysate
may be in
any of the different parts of the product or in only the dry, semi-moist or
wet part. Any
hydrolysed carbohydrate may be in any of the different parts of the product or
in only the
dry, semi-moist or wet part. The feather hydrolysate and any hydrolysed
carbohydrate
need to be in the same part or different parts of the product, e.g. the
feather hydrolysate
may be in a semi-moist centre part and the hydrolysed carbohydrate in a dry
outer shell.
The foodstuff of the first aspect of the invention may comprise feather
hydrolysate at an
amount ranging from about 10-50% on a dry matter basis of the foodstuff.
Preferably, the amount of feather hydrolysate can be at an amount ranging from
about 15-
25%, 20-35% or 30-40% on a dry matter basis.
The foodstuff of the first aspect of the invention may further comprise
hydrolysed
carbohydrate at an amount ranging from about 50-90% on a dry matter basis of
the
foodstuff.
Preferably, the amount of hydrolysed carbohydrate can be at an amount ranging
from
about 60-80% or 65-75% on a dry matter basis.
The foodstuff of the first aspect of the invention may be administered to a
dog in a range
of an amount containing from 0.1 to 1g of feather hydrolysate per kilogram of
dog.
Preferably, the amount of feather hydrolysate can be at an amount ranging from
about
0.2-0.5g, 0.3-0.6g, 0.4-0.8g, 0.7-1g of feather hydrolysate per kilogram of
dog or any
amount of about 0.1g, 0.2g, 0.3g, 0.4g, 0.5g, 0.6g, 0.7g, 0.8g, 0.9 or 1g of
feather
hydrolysate per kilogram of dog.
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The foodstuff of the first aspect of the invention may be administered to a
dog in a range
of an amount containing from 0.5 to 4g of hydrolysed carbohydrate per kilogram
of dog.
Preferably, the amount of hydrolysed carbohydrate can be at an amount ranging
from
about 0.5-2g, 1.5-3g, 2.5-4g or 1-3.5g of hydrolysed carbohydrate per kilogram
of dog or
any amount of about 0.5g, 1g, 1.5g, 2g, 2.5g, 3g, 3.5g or 4g of hydrolysed
carbohydrate
per kilogram of dog.
The foodstuff of the first aspect of the invention may have any ratio of
feather hydrolysate
to hydrolysed carbohydrate from 5:1 to 1:10. Preferably, the ratio: 3:1 to 1
to 5.
Preferably, the ratio is 1:4.
The present invention relates, for all aspects, to a dog that is in need of
improving
performance during exercise and/or improving recovery after exercise.
The second aspect of this invention provides a method for improving
performance during
exercise and/or improving recovery after exercise. In particular, the method
comprises
administering to a dog a foodstuff which comprises feather hydrolysate (the
foodstuff of
the first aspect of the invention).
Further, the method is preferably administered to a dog, in particular a
working dog after
exercise to improve performance of the next exercise. Reducing muscular and
cellular
stresses improves the performance in the next exercise.
The foodstuff of the first aspect of the invention can be administered before
or after
exercise. The foodstuff may be administered 10 minutes, 20 minutes, 30
minutes, 45
minutes, 1 hour, 1.5 hours or 2 hours before or after exercise or immediately
after the dog
stops to rest after exercising, within 10 minutes, 20 minutes, 30 minutes, 45
minutes or an
hour after exercise.
The foodstuff may be administered in a dietary regime in accordance with the
usual
dietary regime of the dog. The foodstuff may comprise 100% of the diet of the
companion
animal or a lesser proportion, depending on the level required. The foodstuff
can be
administered by the animal's owner or trainer. The foodstuff may be available
at any
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outlet selling pet food products or may be available from a veterinarian. The
foodstuff may
be as described above according to the first aspect of the invention.
As used herein, the term "administration" also includes feeding or any other
method of
oral administration.
The foodstuff of the second aspect is as described for the first aspect of the
invention
(above).
Preferred features of the first aspect of the invention apply as for the
second aspect of the
invention mutatis mutandis.
In a third aspect of the invention there is provided a foodstuff comprising 10-
50
% of feather hydrolysate on a dry matter basis.
Preferred features of the first and second aspect of the invention apply as
for the third
aspect of the invention mutatis mutandis.
Preferably, the amount of feather hydrolysate can be at an amount ranging from
about 15-
25%, 20-35% or 30-40% on a dry matter basis.
The foodstuff according to the third aspect of the invention may further
comprise
hydrolysed carbohydrate.
The foodstuff may further comprise hydrolysed carbohydrate at an amount
ranging from
about 50-90% on a dry matter basis of the foodstuff.
Preferably, the amount of hydrolysed carbohydrate can be at an amount ranging
from
about 60-80% or 65-75% on a dry matter basis.
The foodstuff of the third aspect of the invention may have any ratio of
feather hydrolysate
to hydrolysed carbohydrate from 5:1 to 1:10. Preferably, the ratio: 3:1 to 1
to 5.
Preferably, the ratio is 1:4.
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The present invention also relates to a method of enhancing endurance and
prolonging
stamina of a dog during exercising includes sustaining performance or boosting
the dog's
energy to perform tasks during exercise in particular successive exercise.
The present invention also relates to a method of improving performance and/or
improving
recovery, includes reducing tissue and cellular stresses in the dog's body,
for example
reducing muscle damage, reducing inflammation, reducing oxidative stress
and/or
reducing body temperature.
The present description includes a method for preparing the foodstuff of the
invention.
The foodstuff can be made according to any method known in the art, for
example as
described in Waltham Book of Dog and Cat Nutrition, Ed. ATB Edney, Chapter by
A.
Rain bird, entitled "A Balanced Diet" in pages 57 to 74 Pergamon Press Oxford.
For example, a process for the manufacture of a foodstuff as defined herein
comprises
mixing together ingredients with the composition comprising feather
hydrolysate and
forming a foodstuff, in particular a pet foodstuff. Heating/cooking may be
applied to any
one or more of the ingredients prior to, during or following the mixing.
The feather hydrolysate and/or hydrolysed carbohydrate can be sprayed onto the
foodstuff, mixed in with the foodstuff or incorporated into the foodstuff in a
matrix. Methods
of inclusion of the feather hydrolysate and/or hydrolysed carbohydrate
composition are
known in the art.
The invention will now be further described by way of reference to the
following Examples
and Figures, which are provided for the purpose of illustration only and are
not to be
construed as being limiting on the invention.
Figure 1: shows a schematic diagram cross over design (n=10) of the design of
the trial.
The figure shows the stages of exercise (T0-T1, T2-T3 and T4-T5) stages of
rest (T1-T2, T3-
T4 and T5-T6), time of blood sampling (-10, T4, T5 and T6) and time of
supplementation (T1,
T3 and T5). One exercise consists of 5 intensive sprints (100m), 20 min of
endurance at
20 km/h and 5 intensive sprints (100m). Each dog is its own control (C) 2
weeks later
when the dog repeats the same exercise and is supplemented with the foodstuff
of the
invention (S).
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Figure 2: shows the results obtained when assessing muscle damage. Figure 2a
shows
the results obtained when assessing Pro BNP. Figure 2b shows the results
obtained when
assessing creatine kinase.
Figure 3: shows the results obtained when assessing inflammation. Figure 3a
shows the
results obtained when assessing C Reactive protein (CRP). In figure 3b, 3c,
3d, 3e, the
letters in lower case are used to assess the effect of check point (time) for
the control
group (within group comparison). Upper case letters are used to assess the
effect of
check point (time) for the supplemented group (between group comparison).
Different
letters show significant difference at 5%.
Figure 3b shows the results obtained when assessing Myeloperoxidase (MPO).
Figure 3c
shows the results obtained when assessing TNFa. Figure 3d shows the results
obtained
when assessing interleukin 6 (IL6). Figure 3e shows the results obtained when
assessing
interleukin 10 (IL10).
Figure 4: shows the results obtained when assessing oxidative stress. Figure
4a shows
the results obtained when assessing Advanced Oxidative Protein product (AOPP).
Figure
4b shows the results obtained when assessing the ratio of reduced glutathione
(GSH) to
oxidized glutathione (GSSH). In figures 4a and b, the letters in lower case
are used to
assess the effect of check point (time) for the control group (within group
comparison).
Upper case letters are used to assess the effect of check point (time) for the
supplemented group (between group comparison). Different letters show
significant
difference at 5%.
Figure 5: shows the results of internal body temperature of control (C) and
supplemented
(S) groups. Figure 5a shows the results of the body temperature at each
interval in the
trial. Figure 5b shows the mean average results of body temperature between
the control
(C) and supplemented (S) groups.
Example
Ten dogs belonging to the French army were used in the trial. All dogs were
fed with the
same diet (Profine ); comprising 33% crude protein, 22% crude fat, 2.5% fibre,
10%
moisture, 7% ash, 1.6% calcium, 1.2% phosphorus plus Vitamins A, D3, E,
Copper, Zinc
and Selenium, Omega 3 and B. Total Metabolized energy = 4,379 kcal / kg.
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All dogs had the same body score, were homogenous in age (2 to 5 years) and
were
trained the same way for at least two months.
Each exercise consisted of 5 intensive sprints of 100 meters, 20 minutes of
endurance
running at 20km/h and 5 intensive sprints of 100 meters.
The intensive stamina test is composed of resistance and endurance exercises:
- going in front of the dog for 50 meters and coming back to the handler after
grabbing a tool by an assistant man, repeated five times while being timed;
- running for 20 minutes at constant speed (20km/h);
- going in front of the dog for 50 meters and coming back to the handler after
grabbing a tool by an assistant man, repeated five times while being timed.
Dogs performed the stamina test three times within a day with one hour of rest
between
each session. All dogs perform the trial twice (once as control (C) and once
as
supplemented (S) separately at least for 15 days) so that each dog was its own
control
Unlimited water was provided before the exercise and during the recovery
period. A
standardized quantity of water was provided taking into account the weight of
the dog at
the time of administration of the placebo or the supplement.
The ten dogs were divided in two groups to allow a cross-over design (each dog
was its
own control):
-A: dogs from this group did not eat the supplement (C);
-B: dogs from this group ate the supplement (S).
A supplement was fed to the dogs after each exercise at Ti, T3 and T5; see
figure 2).
The dogs in group A were fed 150 ml of water as a placebo (control).
The supplement fed to the dogs in group B consisted of the following powder,
instantaneously dissolved in 150m1 of water:
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- 75% of maltodextrine (IT 18) at 1.5g of maltodextrine/kg of dog
- 25% of feather hydrolysate (Protamine, BCF) at 0.5g of feather meal/kg of
dog
- Mixed in 150 ml of water.
Statistical Analysis
Data were analysed using the mixed procedure of SAS version 9.3 software (SAS
Institute
Inc., Cary, N.C., USA). The fixed effects were: group effect in 2 levels
(supplemented/control), check point effect either in 16 levels for
physiological parameters
(TO, Ti, T1+10min, T1+20min, T1+30min, T2, T3, T3+10min, T3+20min, T3+30min,
T4,T5, T5+10min, T5+20min, T5+30min, T6) or in 4 levels for blood parameters
(TO, T4,
T5, T6) and the interaction Group effect x Check point effect. Sprinting time
was added as
a covariate in the statistical model to adjust the analysis to sprinting
performance.
Specifically for Interleukin 10 analysis, the value of interleukin 10 within
group at TO was
added as covariate.
Dog was defined as a random term to fit with the cross over design (data
pairment).
According to the distribution of residuals of the statistical model, variables
were previously
logarithmic transformed (Interleukin 6 parameter) or not and ranked (creatine
kinase
parameter) or not. Level of significance was set at 5%. A trend noticed T in
the graphs
defined a P-value between 5 and 10%. One star "*" in the graphs defined a P-
value
between 1 and 5% (significant effect). Two stars"' in the graphs defined a P-
value
between 1 and 0.1% (very significant effect). Three stars"*""in the graphs
defined a P-
value between under 0.1% (highly significant effect).
Results
Four blood samples were performed to see the effect on inflammation, oxidative
stress
and muscle damage: before the first exercise (TO), before the last exercise
(T4 :
encompassing the 2 first exercises), at this end of the last exercise (T5) and
one hour
after the end of the last exercise (T6).
Blood samples to measure lactates, CKmm, inflammatory, oxidative stress, and
biochemistry parameters was performed at the beginning of the test (TO),
before and just
after the third exercise (T4 and T5), and 1 hour after the last exercise (T6).
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Parameters studied Biological measured
Physiological parameters Heart and respiratory frequencies [data not
shown], body
temperature.
Biochemical markers Urea, creatinine, total protein, glucose,
triglycerides, Na+,
K+, lactate, albumin. [data not shown]
Muscular damage marker Creatine Kinase, Pro-Bnp
Inflammatory markers MPO, cytokines (IL6, ILI 0, TNFa)
Oxidative stress marker AOPP, GSH/GSSG, MPO
Physiological parameters were measured before all exercises periods, and for
30 minutes
after the end of exercises (just after, 10 minutes after, 20 minutes after,
and 30 minutes
after).
Muscle damage
The results show that supplementation reduced muscle damage measured with
several
markers.
Pro-BNP is a marker of heart cell stress and more globally of muscle damage.
Supplementation tends to decrease this marker level whatever the check point
considered
(P=0.054). This can be seen in figure 2a.
Creatine kinase is released into the blood when tissue is damaged due to cell
lysis and
therefore indicative of muscle cell stress. Supplementation significantly
decreases this
marker level especially after the two first exercises (T4; P=0.002), and at
the end of the
third exercise (T5; P=0.022). One hour the last exercise the difference
between groups
tend to remain in favour of supplementation (P=0.09).This can be seen in
figure 2b.
Inflammation
The results show that supplementation reduced inflammation.
C Reactive Protein (CRP) is a marker indicative of inflammation.
Supplementation highly
decreases this marker level at the end of each exercise (P<0.001). CRP in the
supplemented group was reduced by 50.25% compared to the control group
whatever the
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check point considered (no significant interaction between group and check
point effects).
This is shown in Figure 3a.
Myeloperoxydase (MPO) is a marker indicative of inflammation and oxidative
stress.
Supplementation reduces very significantly inflammation and oxidative stress
after the 2
first exercises (T4) compared to control group and reduces highly
significantly
inflammation and oxidative stress after the last erxercise (T5) and one hour
after the last
exercise (T6) compared to control group. Although inflammation increases at
the end of
the third exercise compared to basal state in the control group, the
inflammation level
stays at the basal level for the supplemented group. This is shown in figure
3b.
TNFa is a pro-inflammatory cytokine produced by T cells and macrophages during
the
inflammation process. Supplementation significantly reduces inflammation one
hour after
the last exercise compared to control. This is show in figure 3c.
IL6 is a pro-inflammatory interleukin produced by T cells and macrophages
during the
inflammation process. It is also a myokine: a cytokine produced by muscle
during
contraction. Moreover, in a human it has been shown that depletion in glycogen
storage is
linked with an increase in IL6 level. Supplementation significantly reduced
1L6 level
(Inflammation) one hour after the last exercise compared to control. This is
indicative of
reducing inflammation, as well as reducing depletion in glycogen storage in
the muscles.
This is shown in figure 3d
11_10 is an interleukin produced during inflammation. Supplementation highly
significantly
reduces 11_10 level at the end of the last exercise (P<0.001) and one hour
after the last
exercise (P<0.001), indicative of reducing inflammation. This is shown in
figure 3e.
Oxidative stress
The results show that supplementation educed oxidative stress.
Advance Oxidation Protein Product (AOPP) is a marker of protein oxidation (a
type of
oxidative stress). Supplementation very significantly reduces AOPP level at
the end of the
last exercise and highly significantly reduces AOPP level one hour after the
last exercise
compared to control, indicative of reducing oxidative stress. This is shown in
figure 4a.
CA 02915006 2015-12-10
WO 2014/202772 PCT/EP2014/063060
A decrease of the ratio of reduced glutathione (GSH) to oxidized glutathione
(GSSG) is
considered indicative of oxidative stress. Supplementation highly
significantly increases
the ratio of GSH/GSSH at the end of the last exercise and one hour after the
last exercise,
indicative of reducing oxidative stress. This is shown in figure 4b.
Temperature
A significant reduction of temperature during exercise is also observed with
the group of
dogs which were fed the supplement. Indeed the supplementation permits to
decrease
body temperature by 0.2% during exercises, this is statistically significant.
This is shown in
figure 5.
Conclusion/Discussion
The results showed that supplementing a dog with the foodstuff of the present
invention
after each exercise improved their subsequent performance and improved their
recovery
after each exercise. Biomarkers relating to muscle damage, inflammation and
oxidative
stress in the blood samples taken from the dogs supplemented with the
composition of the
present invention were shown to be reduced compared to dogs not supplemented
after
exercising. In addition, it was shown that the body temperature of the dogs
was also
reduced.
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