Canadian Patents Database / Patent 2649145 Summary

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(12) Patent: (11) CA 2649145
(54) English Title: A METHOD OF PRODUCING SELENIUM-ENRICHED YEAST PRODUCTS, AND USES THEREOF
(54) French Title: UNE METHODE DE PRODUCTION DE PRODUITS DE LEVURE ENRICHIS AU SELENIUM ET LEURS UTILISATIONS
(51) International Patent Classification (IPC):
  • C12N 1/16 (2006.01)
  • A23L 33/14 (2016.01)
  • A23L 33/16 (2016.01)
  • A61K 33/04 (2006.01)
  • C12N 1/18 (2006.01)
  • A21D 8/04 (2006.01)
(72) Inventors (Country):
  • MIROSHNYCHENKO, OLEKSANDR (Canada)
  • MIROSHNYCHENKO, ZORYNA (Canada)
(73) Owners (Country):
  • MIROSHNYCHENKO, OLEKSANDR (Canada)
(71) Applicants (Country):
  • MIROSHNYCHENKO, OLEKSANDR (Canada)
(74) Agent: NA
(45) Issued: 2013-09-24
(22) Filed Date: 2008-12-22
(41) Open to Public Inspection: 2010-06-22
Examination requested: 2009-02-17
(30) Availability of licence: N/A
(30) Language of filing: English

English Abstract


The present invention describes a process for preparing selenium-enriched
yeast products
that have high contents of organically bound selenium. It is achieved by
adding the selenium
compound to the nutrient medium wherein formation of multiple enzyme-metal
complexes is
optimized, which increases the maximum rate of the fermentation process and
boosts the
concentration of organic selenium in these products. The invention also
provides a means for the
production of practically non-toxic selenium-enriched yeast products for use
in foods, dietary
supplements, or drugs.


French Abstract

La présente invention décrit une méthode de production de produits de levure enrichis au sélénium qui ont une teneur élevée en sélénium organique. La méthode est réalisée en ajoutant le composé de sélénium au support de nutriment où la formation de multiples complexes enzyme-métal est optimisée, ce qui diminue le taux maximal du processus de fermentation et augmente la concentration de sélénium organique dans ces produits. L'invention fournit également une méthode de production de produits de levure enrichis au sélénium pratiquement non toxiques pouvant servir dans les aliments, les suppléments alimentaires ou les médicaments.


Note: Claims are shown in the official language in which they were submitted.

16
What is claimed is:
1. A method for producing a selenium-enriched yeast products having an
organically bound
selenium content of between approximately 3000 ppm and 5000 ppm, calculated on
a dry
matter in the yeast, comprising the steps of:
(a) preparing trace element composition for a 5 L nutrient medium as NaCl
(0.2045 g),
KH2PO4 (0.2755 g), CaCl2 .cndot 2H2O (0.2054 g), NH4Cl (2.1094 g), MgSO4
.cndot 7H2O
(1.3662 g), FeSO4 .cndot 7H2O (0.567 mg), Na2B407 .cndot 10H2O (18.55 µg),
ZnSO4 (1.074
mg), CuSO4 .cndot 5H2O (0.375 mg), Co(NO3)2 .cndot 6H2O (29.10 µg),
(NH4)6Mo7O24 .cndot 4H2O
(0.2649 mg), NiSO4 .cndot 6H2O (10.52 mg), MnSO4 .cndot H2O (1.014 mg), KI
(0.1667 mg),
biotin (0.60 mg);
(b) preparing an aqueous yeast grown fermentable sugar containing nutrient
medium in
which molasses is the sole carbon source;
(c) adjusting the pH of the nutrient medium to 4.9-5.0;
(d) adjusting the temperature of the aqueous nutrient medium to 37-38
°C;
(e) adjusting the aeration rate to the nutrient medium;
(f) adding yeast to the nutrient medium;
(g) attaining a pH of about 4.45, at which formation of multiple enzyme¨metal
complexes are optimized;
(h) preparing an aqueous solution of selenite salt;
(i) adding the selenite solution in the range of 0.13 to 0.20 g/l to the
nutrient medium at a
pH about 4.45;
(j) cultivating for a period from about eight (8) to about (18) hours while
gradually
decreasing the pH to about 4.0;
(k) adjusting the pH with ammonium hydroxide to 4.6-4.7 when the pH of the
fermentation reaction reaches 4.0 during cultivation;
(l) harvesting the selenium yeast product and concentrating it by
centrifugation in order
to separate the selenium yeast product from the nutrient medium; and
(m) washing the selenium yeast product.
2. The method of claim 1, further comprising a step of drying the washed the
selenium yeast
products for use as large-scale baker's or other manufactured yeasts.

17
3. The method of claim 1, further comprising a step of pasteurizing and drying
the washed
the selenium yeast products for use in dietary supplements and drugs.
4. The method of claim 1, wherein said harvested selenium-enriched yeast
products are
washed until achieve a negative reaction with SnCl2, which indicates that
water-soluble
residual selenium compounds are removed.
5. The method of claim 1, wherein said selenite salt is Na2Se2O3.
6. The method of claim 1, wherein said the yeast growth on carbon source
comprises one of
the members of the group selected from beet molasses or cane molasses.
7. The method of claim 1, wherein said the yeast growth carbon source
comprises a mixture
of beet and cane molasses.
8. The method of claim 1, wherein said yeast selected is baker's yeast
(Saccharomyces
cerevisiae).
9. The method of claim 1, wherein said yeast selected is brewer's yeast
(Saccharomyces
uvarum).
10. The method of claim 1, wherein said the airflow rate on oxygen transfer
for a 5 L nutrient
medium is 9 L/min and agitation rate is 250 rpm.


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Admin Status

Title Date
(22) Filed 2008-12-22
Examination Requested 2009-02-17
(41) Open to Public Inspection 2010-06-22
(45) Issued 2013-09-24

Maintenance Fee

Description Date Amount
Last Payment 2016-10-25 $100.00
Next Payment if small entity fee 2017-12-22 $100.00
Next Payment if standard fee 2017-12-22 $200.00

Note : If the full payment has not been received on or before the date indicated, a further fee may be required which may be one of the following

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  • the late payment fee set out in Item 22.1 of Schedule II of the Patent Rules; or
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Payment History

Fee Type Anniversary Year Due Date Amount Paid Paid Date
Filing $200.00 2008-12-22
Request for Examination $400.00 2009-02-17
Maintenance Fee - Application - New Act 2 2010-12-22 $50.00 2010-10-07
Maintenance Fee - Application - New Act 3 2011-12-22 $50.00 2011-10-26
Maintenance Fee - Application - New Act 4 2012-12-24 $50.00 2012-10-18
Final $150.00 2013-07-03
Maintenance Fee - Patent - New Act 5 2013-12-23 $100.00 2013-12-17
Maintenance Fee - Patent - New Act 6 2014-12-22 $300.00 2015-11-16
Maintenance Fee - Patent - New Act 7 2015-12-22 $100.00 2015-11-16
Maintenance Fee - Patent - New Act 8 2016-12-22 $100.00 2016-10-25

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Abstract 2008-12-22 1 19
Description 2008-12-22 15 826
Claims 2008-12-22 2 68
Cover Page 2010-06-07 1 34
Description 2009-02-17 15 815
Abstract 2011-05-09 1 14
Claims 2011-05-09 2 49
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Description 2011-05-09 15 790
Abstract 2011-10-26 1 15
Description 2011-10-26 12 652
Claims 2011-10-26 2 58
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Abstract 2012-04-05 1 15
Description 2012-04-05 12 636
Claims 2012-04-05 2 59
Abstract 2012-12-18 1 15
Description 2012-12-18 15 797
Claims 2012-12-18 2 72
Drawings 2012-12-18 1 16
Cover Page 2013-08-29 1 31
Prosecution-Amendment 2011-03-01 9 559
Correspondence 2009-02-05 1 23
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Correspondence 2011-01-04 2 89
Correspondence 2010-12-09 2 83
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