Note: Descriptions are shown in the official language in which they were submitted.
^~ Milk co~tains one or more proteins o~ ~he globulin
group although the amount present is usually quite sm~ll. The
globulin group is generally considered to be comprised of
lactoglobulin or beta lactoglobulin. The globulin of milk is
very important. Under certain conditions it enables a mother
to transmit immunity toward certain infections to her suckling.
' This takes place during the first 2 or 3 days of the young ani-
~ malls life.
- During the first few days of lactation, the secretion
of the mammary gland differs a great deal from that of normal
milk. The secretion is called colostrum. The first colostrum
is very rich in glcbulin and fills the special needs of the
newborn animal. Among these is a need for globulin, for the
blood of the newborn of many species contains much less that the
normal amount of this protein. Colostrum is an important source
of antibodies for the newborn. The antibodies may either be the
globulins themselves or are associated with the globulins.
Colostrum is generally believed to be important in the case of
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the cow, goat, sheep, pig and mare.
As previously stated, colostrum milk has been shown to
be an important factor in the resistance of infectuous diseases
,~ in the young newborn which was nature's purpose for providing
,; colostrum milk. The antibodies found in colostrum milk help the
,; newborn fight diseases as well as protect them from future
infection through immunal responses. It has also been found that
a cow can be stimulated to produce certain antibodies to bacteria
by injecting the cow before calving with antigens to the anti-
bodies desired. Further, it has been found that a cow which has
been subjected to many diseases during its lifetime will also
;' 30 have produced certain antibodies. When the cow calves, the first
milk produced by the cow will contain antibodies to the stimulants
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: as well as the normal environmental antibodies present in the
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mammalory.
The colostrum milk is collected and the antibodies are
removed therefrom and are manufactured into an injectible solution
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;~1 which may also be used orally to benefit other animals which may
be subjected to the diseases which the particular antibodies are
peculiar to. -
Assuming that the cow has either been injected with cer-
tain antigens or that the cow simply has the normally occurring
antibodies in the colostrum milk, the colostrum milk is collected
1~ 10 after calving. Preferably, the colostrum milk is collected and
:~ frozen until a suitable quantity has been collected to enable the
process to be carried out. The freezing of the colostrum milk
also aids in subsequent removal of the fat from the colostrum
milk. Examples I and II are two examples wherein the antibodies
were recovered from the colostrum milk. Example I is the preferred
~ process of recovering the antibodies while Example II is an alter-
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,;¦ nate method for recovering the antibodies.
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,i EXAMPLE I (PREFERRED )
The colostrum milk is collected and frozen. The milk is
defatted by partially thawing the frozen milk and skimming the
top liquid therefrom. Irhe remaining milk is then completely
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'. ~ thawed and centrifugally separated to remove as much of the remain-
ing fat as possible,
The defatted colostrum milk is then precipitated by add-
,' ing 1.5 mg of CaC12 per liter of milk and by adding one tablet
;` (1.5 gram) of commercially available rennin per liter of milk.
The mi~ture is then thoroughly stirred. The solution is then
heated to 20-80C and again stirred. The solution is permitted to
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. stand for 2-5 hours and the casein in the solution is then removed
by filtration. The resultant solution is termed "bovine colosteral
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whey".
The whey is then dialyzed against tap water at 47F with
saline added for approximately 96 tG 120 hours. The pH o~ the
dialysate is adjusted to 4.0 to 4.5 with acetic acid. The whey
is then precipitated by placin~ the same in vats having a controlled
temperature of 35-65F. The supernatant is siphoned off and cen-
trifu~ed. The supernatant is then clarified by filtration. The
titre of the solution is determined in conventional fashion and a
water, saline and 0.3~ Phenol mixture is added to make a final
bovine IGG concentration of lOOmg~.
The solution is then passed through a water sterilizer
(ultraviolet) and placed in sterile 250 mg bottles and capped in
ultraviolet light. The product is then ready for use.
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EXA~PLE II
The colostrum milk is collected and centrifugally se~arated
to remove as much of the fat as possible.
The defatted colostrum milk is then precipitated by adding
~"~ 1.0 - 2.0 mg. of CaC12 per liter of milk and by adding 0.5 - 1.5
tablets of commercially available rennin per liter of milk. The
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mixture is then thoroughly stirred. The solution is then heated
`~ to 20-80C and again stirred. The solution is permitted to stand
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for 2 5 hours and the casein in the solution is then removed by
,.,.:
; filtration. The resultant solution is termed "bovine colosteral
:, : .
~` whey".
The whey is then dialyzed against tap water at 40-54F
with saline added for approximately 96 to 120 hours. The pH of
~: the dialysate is adjusted to 4.0 to 4.5 with acetic acid. The
; ~ ,
whey is then precipitated by placing the same in vats having a
controlled temperature of 35-65F. The supernatant is siphoned
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'~ 30 off and centrifuged. The supernatant is then clarified by filtra-i, ..................................................................... . .
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tion. The titre oE the solution is determined in conven-tional
fashion and a water, saline and 0.3% Phenol mixture is added to ,-
; make a final bovine IGG concentration of lOOmg%.
` The solution is then passed through a water sterilizer
(ultraviolet) and placed in sterile 250 mg bottles and capped in
ultraviolet light. The product is then ready for use.
:.
The finished product may then be injected into cattle to
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' provide the cattle with the necessary antibodies for disease pro- '
,- tection and immunity. The liquid product may also be formed into
;, lO tablets by conventional methods for oral adminlstration if desired.
Thus it can be seen that the method of this invention i-
accomplishes at least all of its stated objectives.
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