Note: Descriptions are shown in the official language in which they were submitted.
31, 326
NOVEI. E3RO~D 8P~C~R~M ~rIMIcRoBIAL
Thi~ i,veIlltion r~31ate~ to an alkaline basQd
~urfa(:e cleaner having broa~ spectrUDI di~3in~ecta~ ia~d
ao~tailling pi~ oilO
A ~umber of pin~ oil aontaining s~lealler3 ~re
knowIl ~Eor cleani~g h~rd ~urf~Ge~, i.e. poroelain,
enaDIel ~ plastic~ ate~ and tha li~e. æu~h clean~rs
are ~ffeative ~or oleaning greasy soil, and in
combinatioll with Gertai~ ~urfactant~3 th2y have been
ei~Eective i~l cle~ni~g grimy ~oil from su:cfac:e.
however, pi~e oil b.sed oleaner3 hurQ ~ot be~ll found to
ha~e broad spe¢trum anti~icrobial aotivity since they
are o~ly effeotiYe again~t gra~ negative organi~m~ and
~ot against gram posiltive oxga~ism U~ 8 sauater~ary
ammonium c:o~psunas are pr~ ent. Th~ use o~ quaternary
~o~polma~ in a oleaner i~ ~ot desirable 5illl:e the~e
compound~ have a r~umber of unde~irable gualitie~, A
higll r~ lue is le~t o~ the surfaae after c:lea~i~gO I~
addiltiont quaternary ammo~ium co~npou~d are too tonic
for this U~38 ~nd are highly irritati:llg to the skin,
eye~, etc.
Ill IJnit d 8tate~ Patent ~umber 4,867,898, it
is di~closed t~hat a broad spç30trum pine oil
d:L~infec:ta~ay liquid cleaner, efiEeo1:ive against both
gram positive and gram negativ0 orga~lism~, aould be
~ 2 ~ J~
obtained by a co~bination of pi~e oil and oil solllble
organic aaids a~ a pH of O to 6. ~lthough this
formulation ~as a~ efiE6!~tive, broad 8peCtrllm~
~isi~a~ecting c:leanzr, it ~uf~erad frs:m the di3advantage
that it was hars~ to the satin~ eye5 and also to the
surPaGes to be cleaner~ Tlhus, this clea~er wa~ not
entireïy satisfactory ~or general ~leani~g U5e~o
It i~ kno~n that alkaline ba~sed pine oil
~yYtem3 are ~ot so harsh. ~Iowever, it has not
1~ heretofore been pos~ible to obtain ~n alkallne based
pine oil cleaner haviny broad spec:tsum di~in~ectallc:y
against both gram positive and gr~ negative organism~.
8uch formulations were ef:Eeati~e only agai:nst gram
negative ors~anism3.
It is there~ore a~ obj Qat o~ this invention
to provide a pine oil con1:aini~g hard ~urface cleaner
that has broad spectrum disinectancy, but is not harsh
to skin, eyes and the surfa~es bei~g ~lea~ed.
A ~urther objeat is to o~tain a pi~e oil
aontaini~g hard ~Ur~Ge ~leaner~ in an alkali~e medium
having broad spectrum disi~feotanc~0
We ha~e now foun~ that pi~e oil becomes a
true broad spectrum disi~fec~ant in the prese~ce of
small amou~ts~ chelati~g agent and hydrotrope in a
alkaline based e~ulsion produc The raw material in
the amounts thereo~ are non-toxi~, non-irritating, ~o
medici~al odor, compatible with pi~e oil i~ a properly
emulsi~ied system~ co~Yenient and easy to use i~ a
v riety o~ consumer and indu~trial hard surface
disinfe~tant cleaning pro~u~ts~
Numerou~ household, nstitutional,
industrial, and agricultuxal surfaces becom~
cont2minated with bo~h gram negative and gram posit v~
baateria. The presen~e o~ pine sil, tetraso~ium EDTA,
and ~odi~m xylene sulfonate in an al~aline based
.
cleaning preparation will totally diainfect that hard
~urface and deodorize the room without harm to human~
an~ animals.
The ingredient~ and amount~ that mak~ up tha
haxd ~urface cleaner of the pre~ent inve~tion ars
listed in Table I belo~. It will be under~tood that
the listing of chelating agent~ and hydrotropes i~
e~emplary and not in¢lu~ive.
~ 9 ~
TABI.13 I
PINE: OIL DISINF}:C:TING COMPOæITION
WIT~ CON_E~TRA~rION RANGES
INGREDIENTS P13RCENT
Pi~le Oil 10 . 00 to 30 . Ob
Tetra~odium EDTA O . 01 to 5 . 00
Sodium Xyle~-e ~;ul~onate 1. 00 to 15 . 00
I~opropallol 3 . 00 to 10 . 00
Pareth-25 Sulfate 2 . 00 to 16 . 00
Sodiu~ C14-C16 Ole~in sul~onate 2 . 00 to 16 . 00
Li~3ar AlkylBe~ze~l~ æul~o~ate 2.00 to 16.00
Pa~eth 91-8 2.00 tc~ lO.O~
Wa~er ~t.
' - '
::
- ~ D ~ 3 ~
-- 5 --
A~ previou~ly de~cribed, pine oil i3 not
e~estive again~t gram positive organi~m~, although it
is effectiYe against gr~n ~egative organi~m~. It ha~
moreover only been po~ible to obtain di~inf ~¢tancy in
5a pine oil compositio~ against both gram negative and
gram po3iti~re organi~ms in an acid me~ium. It iR,
therefore, surprising that a combinatio~ of pine oil
and a chelating agent,~ an oil soluble organics aci~,~
together with a hydrotrope ha~ broad spe¢trwn
di~infectancy and is effe~cti~r0 against both gram
negative and gram po3itiYs bacterial at a pH o:E 8 lto
20 we haYe :~o~md that this i~ a truly ~ynergi~tic
effect since a composition oo~taining pine oil but no
chelating agent was ine~fe~tive again~t taphylo¢oc~us
aureu~ ~gram po~itive). ~ ~imilar compo~ition
containing pine oil but no hydrothrope wa~ also
i~æ~ f ecti.ve against ~ 0 aureu~ . A ~imil~r compo~ition
with neither chelate ~or hydrotrope wa~ al80
inef~ective again~t SO aureus. Thu~ thi~ compositio~
ha~ broad ~peotrum disinfecta~cy ~i~ce it is ef~e¢tive
agai~st both gram po~itive a~d gram ~egati~e organism~.
Ths pre~ent liquid hard ~urface cleaner thu3
has us~ in hospital~ " duR to it~ broad spsctrum
a~tivity, in i~du~trial u~e and oo~sumer use. q!lle
2 5co~positio~ cordiIIg to the i:nvelltio~, give~
excellent clea~ing o~ grea~y, grimy, fiIlgerpri~lt 2md
~oap ~cum oil~. The combination of pille o;l,
chelati~g agent arld hydrotrope i~ ~afe enc>ugh ~or u~e
a~ a consumer produat.
I~ order to demon~trat2 the . ynergi~tic broad
~pes:trum di in~ec:tanoy e*~ect o~ the clea~er of ~he
pre~ent in~rantio3l, the following ~pecific Example~ are
set ~orth:
6 --
Example~ I to IV
~ ~ormulatio~ ~I) accordi~g to the pre~ent
application was prepared with the ingredient ana
amounts thereof a~ ~hown i~ T~ble I and havi~g a p~ of
betwee~ ~ and 12.
For~ulation~ II, ]~II, IV and V were prepar2d
identical to ~ormulation I, with ~he paramet~rs varied
a3 ~hown i~ Table II,
Exampl0 II
Formulation~ of I to V o~ Example I were
subjected to microbiological te3ting according to the
As~ociation of Official Analytical Chemi~t~ - U~e
dilution Nethod (AOAC-~DM) proedure as used by the
- 15 Environmental Prote~tion Agency (EPA~ and i~dust~y in
ge~3ral, again~t S. aureus. The re~ult~ are ~ho~ i~
Table XI.
~; 20
.
;
- 7 -
,9 ~
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:~: ~
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e ~0 ~
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WD ~ . . . .
E~ C H H H P P
O liil 1-1 H 1-1
i5 0C H
xample III 2 ~
A number of Example~ o~ Formulation I of
Example I were submitted to microbiological te~ting
again~t staphylococcu3 aureu~* (~ynthetic nutrie~t),
5almonella choleraesuiq* ~ynthatic nutrient~,
P~eudomona~ aeru~ino~a~ ~utrient) and E~cherichia
coli* ~nutrient). The resul.ts are ~hown in Table III.
It will be ~een that these formulation~ have
di~in~ectancy activity again~t both gram po~itive a~d
gram negative organism~ and thu~ broad ~pectrum
disinfectancy:
* ~. aureu~ ~ gram po~itiva
8. cholerae3ui~ - gram ~egatiYe
P. aerugino~a - gram negativ~ -
E. coli ~ gram ~egative
9 ~ 3 ~
TABLE III
8UMMARY OF MICROBIOLOGI~AL TESTING OF_~
PINE OI~ BAS~D DISINFECTING COMPOSITION
NOTEBOO~ &
~ICRO. REQUE8T PASS
NUMBERS ORGANI8~ RE8ULT* FAI~
I. 2742-54-31 S. aureu~ 60 /60 P
90-047 gram ~
II. 2709-122-1 S. aureus 60-~60 P
90-051 gram ~
III. 2709-122-~ S. aureu~ 60-/60 P
90-051 gram ~
IV. 27~2-131 g. choleraesuis 60-/60 P
90-134 qr~m -
V. 2742-131 E. coli 20-/20 P
90-134 gram -
VI. 2742-131 P~. aerugino~a 20-~20 P
90-13~ gram ~
* The disi~$ectancy test procedure~ wer~ co~ducted
accordi~g to the Official ~ethod~ of Analysi~ o~ the
A~ociation of OPficial ~nalytical Chemist~ th ~a.
1984~ Chapter ~ Di i~ecta~t~ P~rayraph~ 4.007-4~0~1
~e Dilutio~ Nathod~
9 ~ $
-- ~o --
The unexpected co~binatio~ oP small amount~
of a chelating agent su~h a ~etrasodium EDTA ~0.1%)
and a hydrotrope ~uch a~ ~odiu~ xyl~ne sulfonate tl.5%)
can broad~ the antimicrobial aatiYity of pine oil
(2D%) in an emul~ified ~ystem. In the presenc~ of the
aforementioned chemical specie~, pi~e oil can ~ot
exhibit broad ~pectrum di~3i~fectant activity by
achieving 100% ~ill of both gram positive ~. aureu~)
and gra~ negative ba~teria ~S. cholerae~ui~ ~. coli,
Ps. aerugino~a) as defined by the O~icial Method~ of
~alysis of the aoAc. ~ata in TABLE III veri~ies bro~d
spectxum aotivity a~d TAB~E II establishea the
- combinational effeat of chelant a~d hydrotrope to
potentiate pine oil aetivity.
It will b9 see~ that the combination o~ pine
oil and chelato with a hy~rotrope ha~ a clear and,
qurprisingly, ~y~ergi~tic e~ect i~ providi~g
~ disi~f~cta~cy aotivity a~ai~st both gram po~itive and
- gram negative organi~ms and that a liquid cleaner i~
provided for hard surface~ having a broad ~pectrum
activity whioh i~ ~ot harsh to 3ki~, eye~ and ~ur~aoe~
.
