Note: Descriptions are shown in the official language in which they were submitted.
SENT BY:PENNIE ~ EOMONDS ~ 2e-94 ; 3'45PM ; PENNIE 8 EDMCNDS~ PER!EY-ROBERTSON;# 3
.. .. . .. . . . . . .. .. . ... . .. . . . . . . ..
~ 2.~.30~97~
WO 9~ J23035 P~/A'r94f O0039~ :
~bln~nt ~y~
ara~ ~9 th~ t ~ear~t ~ t llorols~
o~currlng durln~ th~ ~u.. . ~ thul 20~ o~ lv~ 'co
l~ort~t or~ n ~lorgen- Lnclud- Oro~ I t1~, ~up V ~2~, Cro~p
dl-oo~d to ~ ~r-rs pollo gu~ ~7~ 3)- ~
allorg r~o ~$~ nd ~o l~ologLe-lly or ~truo~ ,ly olo~ly
rol-tot ~ poll~ o~ a~f-r-At gr~ ~lo~, ~d r-l~t-t
~11~ o~ o~o group ~h~w oro~- rewtlv~ty wlth ~tl~ 50 ~r,
~1~ D~ th~ .~ ~ ~ L~ tod b~r r~bl~t ~hnl~
~n~ ps~ ooll ~9~. ~ny o~ t~ ~llor~n~ ~pr-~ ln
E oolJ how ~ro~ r to th~- o~ tu~ rot L~, ~na c~
th-~nlor- b us d ~Dr ~ho dl~o-L~ u~d th-r~y Or llor~l~ dL~
(10, 11, 11). Jl~, do~crl~d ~os t~ ~ t tl~ 1- ~ol oul~
ohnru~ elon o~ o~plolt oD~A rhloh aod for Pbl p I~, ~nd th
lon o~ ot-Ln ln ~. ooll. ~nt~l no~ t- ~ca~bln~nt
~rou~ rI~III or~u ~ ll~n q~ lhbl~ d, ~o o n ~ ~:
o_n ~rom th ~pl~u, Lt o~n ~ u~ llho t~ n~tu~ ro~ln ~or : -
b--~ on ~ ntl~o~y ll~t~r~tlol~, uoh ~e ~ u--t ~o~ :
o ~lol~ ~OO-U~ UJ- .11 S-~ll ~Lto~o r ~nt ln tl~
s~ln-nt ~o~oaul-, ln th- n tur-1 ~l-cul- rurt~or ~ th
r~o~blsu~t ~1~1 p 11 1~ eult~1~ for proo ~ whloh s uult ln
~ur bl- ~dL-to~ r-l--~e. ~ th~s-~tlo u~- Or r~o~ bl p
I~, b-od on ~n d~ ~t on L~u~or gul~tory ~roo ~ , ~e~n-~At~body
lnt-r-ctlon, S-o-11 r~tlvlty ~d ~odl~tor re1-~u~, L~ th con-4uon4-
Or t~ ot~otur~ u~d blolo~lo~l o~l1 rlty ~tw n th- ~tur-1 ~d th-
r-oolobln~nt ~-~ tr-~e~t l~ Atlon ~1~- ~ ca~pl~ oD~la
~hloh cod-- rOr ~ bl~t ~hl p II ~ll~q~ 01~ th b l- or tho
~no ~ald ~u~ ~duo d fro~ eh~ cD~ c~ d ~c-ll ~lto~ o~
tb- ~hl p II nll-rg~- ~ dot ~l~d Th r-oo bl~nt ~1 p ~S
~llor~n n- pr~d Ln ~ ~oll ~ h~- ~rop rtl~ r to tho~- o~
~urnl Oroup ~ S~ gr~ oll n 110r~no ro~ ~hl- lt ~ollow~ th-t
~ho r~ombln~nt ~hl p lI ~ rg~n, ~Llko th- n~tus-l Group II/III
ll~n-, o~n b- u--q ~oo pr~ o- ba~d on nt~n--ntl~o~y
13to~ot~on, ntlg~n~ nt T~-11 ~Lon or ~tLg~n~nd ~t -~
- ~: .
SENT BY:PENNIE ~ EDMONDS ~ 28-94 , 3:5~PM ; PENNIE ~ EDMONDS` PERLEY-ROBERTSON:# 7
. .
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Z1!~ 7~
m~l~or rdo~-e, wh~r~, ~ow~vor~ the r~oa~lbLn~nt Ll~r~n~ ha~ th~
~tdltlonal aav~nta~ of g~nt~r ~urlty and hlqhor ~ lol'cy.
4toril-1 ~4 thod~ ~
: ~
~rl~othy qr4~1~ pl~ar, ~All~rgon ~ ~nq~lhol~, Bw~n), whloh h~,d ~e~n
ln ~lgated ~or E~urity ~y ~ n~ o~ llght ~loro~co~y n~ l ctro~
~ro~o~y, ~aa ~ood ~or tho l~olat~n of ~oly d-nyl~tod lINA ~13). ODI~
~ynthanl~ ua- oarr ~ d out w~tb oll~ nd ~ ri ~ r~; tho end~ o~ ~ -
th oD~A ~ro dl~nntod wlth ~4 poly~4r~- and provldad wlth ~ooXI
l~n~or~ Th~ l~nb r~d oD~A wa- llg t-d lnto do~ho-~horylatod l~bd~
gtll r~b nd ~ d A oDNA g~a lLbrary o~ ~DO~OOO indYp~n4~t
clono~ w~- obtaln-d ~
: . ~ -: '
na~y~l-
~ ,~
Iot ~r~-n~n~ of tho tl~othy ~ra~- Fol~on ~D~A o-n lLbrary w o carsid
out ~ d-ocr~b d ~y ~rolt-n-t-r t al ~14) S~-bin413g olo~-~ w~r-
~rl~ d and gha~a-DNa w~ ~r-p r-d ~rc~ th-~- clon-~ ) 3y ~oanr of
~pn I ~n~ ~o I cut- t~o D~A ~r 4m~t~ u~r- obtaln-~ whloh oont-lned
hoth ~ortlon~ o~ th oo~pl t- ~h~ ~ IS OD~A ~d ~I-nkln~ lambd- Ctll
~-~u Do-o~ Th r--~ltlng ~p I/~-c~ nd B~cl D~A ~r~gm nt- w-ro -~
rubclo~ d lnto th pl~-mld p~C 18~ ~nd tr~n~ormed ~nto ~ coli ~
~lu~ 9ult-bl- clonn~ w~r ld ~tlfled by re~trlotlon ~n~lyc$~ ~nd bcth
~tr~nd~ wYr- r-qy-nc-d by mo~n- o~ l~mbG~ ~t11 forw~rd oqu nclng prlm r
~2~ r) 4n~ l rbd- gtll r~v~r~ qu~ncln4 prlm r ~22-m~r~, Clo~t-ch
~bor-to~o~, P~lo Alto~ ~A, ~nd by m~ nG o~ H13 ~Cl~ forw rd ~nd
ro~ r~ rl~ r-, ~o-hrlngor-~n~h-im, ~ rm~ny, ~ccordLng to ~n4~r
16~. -' ' '
~ RNA_L~o~th4~ blot~
10 ocO o~ tot~ A fro~ poll-~ ol tl~othy yr~-~ rPhl-un pr ton~eJ nd
ry~ g~ r~ollUS P-r-n~-) w~ p~r~t-d by den~turln~ gel elec~o-
~hor~ nd blotte~ onto nlt~oc-11ulo~e ~17~ ~o l~ol~t- the ~DNA
codlng ~or ~bl p II, the oorse~pon~lng D~A ln--rt w~ u~plifl d by ~CR,
~tnrtlnq ~rom r-oo~bln nt ph y~ 5 pioo~ol-- o~ primer ~ bd- gtll ;~
~osw ¢d -quonciAq ~ri~ r l~2-~er~ ~na la~b~4 ~tll r~ o ~eqY-noln~
S ' ~'
., ; .~
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SENT BY:PENNIE ~ EDM~NDS ;11-23-94: 3:51PM; PENNIE ~ EDMONDS~ PERLE~'-R3~ERTS~N;# ~
-- ~ .
7~
prl~r ~2~r~, Clont~h ~oro,tor~o-, ~3A~, w~o uond ln oaoh cao~.
Th~ PCR pra~lat WB~ l~ to 14 6~7Qro~ ~ol ~.nd th~ b-nd~ w~r nlute~d
~y ~an~ of ~ DJAII loll ~ch~ngor p~r ~ . T2~ D~IA oil~taln~d w~ ~
l~l-d by r~ndara ~ 13). Prohybrldi~lon an~ h~brldl~-'clon woro
carrlod o~t l~y ot~d ~ho~o ~15). ~h~ blot~ ~re .t~.hea ~Lth
3.0 ~ 88C ~20 x ~sC ~ 3 11 ~aCl, O.g M ~ altrato, p~ 7.0), O.lt 5D8
(~odl~u~ dod-ayl oul~llto~ 1.5 ssc, 0.1~ t nd ~ lly wlth 0.75~ sW~
0,10 8D~ at 50'~, nd th~ utor~ r pb~d t~f~ ~, A~rd~
L~don, m~ .
l~y ~Qn~ o~ Igll ~or~nq ~ t-- oD~ oloo-- ~-o c~t~ w~ch
~or ~ Phl ,1~ llo~n. Sho 1Y~Og~A1C J!. coll l~t;ral~ Y 108q var
~l~td w1th r~coo~l~nt l~bd~ g~ll ~d t~o p-pl-Gtoeld~e ~u~
prot~ln w~.~ obt~lno~ fro~ tho ~ch ~ao~. Tho b~tch w~ rat~
oSrop~o~n~ y ln n 7.5~ yu:rylu~d~ and blott~ onto
~rocollulooe ~Z2). Th- ~u~lon ~r~tolD w~ toot~ by ~s o,~
Igl~ o~ lont- ~llorgLo to ~ n ~ n l,odL~ l d r~lt
antlhu~n Ig8 ne~body ~Ph~ol~, ~aala, 8~).
Th- att-c2~ Flgur ~ ~orvo to lllwtr-t- thO u-~billty o~ tl~
r~a~ AZ p rl all~ ~ lor ~ro~-~o~ d or~ ~tlgon-~t~
lnt-ra~tLon, ~,ntl~n-d4~n~nt m~tor rQlo~, ~d nt~n-~ndant
c-~.lul~ r~ot~vL~y. ~9. 1 ~ th~ A o~ t~o r ~rb~nt Phl p rI .
llg rl ~d tho l~tt-r'~ cld so~ duood ~r~ ~ld DNlL
Th- oa~lot- cD~ ~u~- o~ tl~O PAl p II tl~ lo oho~ b~--
nonoodln~ o~-nc- -o ~ound ~t th 5~ ~nd Of th ~DNa, whloh 1-
~ollo~d ~y 7~-bn~- lo~,dor ~ na-, ~hloh oodoo ~or th~ lgnnl
~tld undll~d L~ tho rl~ ~23). Sh ~lno ~c~d ooquonoo of th~
~tur proto~ ~ d duc-d rroD ba~ ~8 on, ~ b gln- ~lth v~l~. ~ ~
Th~ atop oodo~ ~AO, whlob b~-~lu Or~ tbo codlns~ t tho 3~ ond, ~ ~;
~ot~ t~lc~c. Th 3 ncodlng ~-~o~ -nd- lr tho ~oly A
rLg. 2 oho~- th~ ty o~ tl~ ~lno ~cl~ o- o~ th- . -
~conbln~nt Phl p 1~ ~llorg~n to oth-r Oroup II/IIT cr~o~ n ;~
: .:
: :-.
SENT BY:PENNIE J ED~ONDS ~ 28-94 ; 3:51PM ; PENNIE ~ EDMONDS~ PERLEY-ROBERTSON;# 3
2~ ,g7~ ~
~ . 3 preo~tE th- d~-criptl~n o~ ll ~ltope9 of tho rooombl~ t
PAI p 1~ ollor~-n~ ~d Flg. 4 th~t o~ th~ S-CQ11 opltOp~-
~
Illg. 5 ~ tho ro~ot~rlty o~ tho r~oaablnu~t Phl ,p Il ~ rg n with
~orum ~g~ o~ ~ti~ nll~ to ~ ~oo~ln~t Pbl p I~ ~
r~ c~ ~ ~ l~to~ u~lon ~Iro~ 2f~o~nlo J. ool~ - .
Y Ioa9 by ~h~g ~ootl~ ~ Im~u~lo~ Ylth l~TO. ~h~ oll
~rotolno; ~vhlo~ oo~t~ln t~ r~o~ PhZ p Tl, w ~ el-~tro~
~hor-tlcnllr ~-~r~ted ~d tr~ ~rd o~to nJtroucll~loro ~oul
1 w ~ I~ o~ ~ O~Ou~ SI~S21-r- otl~e patl~tl ~llo ~ ~
2 ana 3 th d t~l w~- ~on- ulth I~ o~ all rglc ~tLent- ~rho ~ o
r-~tlon wLth Cro~ II/~lI ~11~9~ ~ 4 ~ ~ co~trol
~r~nt wltb ~ o~ ~ non ll~lo ~ont~o~ ~r-o~.
F~g 6 ~re~nt- ~ t bl- whloh ll~to ~ ~c~t4- ~ gr~- ~ollon~
~llorglo ~t~-nt~ wlth ~g~ rowtl~lty wlth ~clfle ~r ~o ~ollor~
~llo~no ~ fr~u~y of r~tl~ltY of gr~s~ ~ollen ~ rglo
pa~clonto wlth ~ r nt 5~r~ rg~ ~ ~lwwn Th~ flg~r
r~-~t pprox~t- valu~a obt~ d by t-ct~ w~ tur~
r o~ln~nt gsar~ r pr ~nt-tlv- ~ ol
~-tl~t~
~1~. 7 ~rov~ th t r~lnnnt Phl p II o~rr1~ th- ~a I0 ~lt~
tl- tho o- wlth~ n~tur~l arOup IS/TI~ r~/Ig~ hlbltlon~ ~ho
of ~ ~tLont wltll gr-o~ pllon ~11 rgy, who ~hh~ Ig~ r--otl~lty
~1~ orou~ ISJ~ ll~n~ /10~ u~ Oroup S ~ rgon~ ~t ubout
30 Im~ w-o ~r~ t-d ~ r~a-bln~nt Pbl p II t~n- 2),-o~bln~nt
Phl p S tJ~ 3~. ~bln nt ~t v I tLu~ 4) or J ooll p~
1) Pr-lnou~-tlon wlth r oo~bln~t ~ p II s~r th Ig~ blndlng ~o
r~t~u~ol JthJ, p II ~hllo-t ca~--t--ly dl~p~ r i~ th 12 kD ~ glon,
wh-r~ ch- r~otlrlty ~lth Phl p I at 30 l~ not ~f-ot~
~oqllbln nt P*l p ~ r~uc-~ o~ly th hlndln~ to n~tur~l Plll p T, bu~ h~
~ rr-ct on th- ~ b~lng to 1~1 p ~ pr lnoub-tlon llth
oontrol p~ot-ln~, r-o411bln nt ~-t v s ~ '. coll ~rot-;lnr ~oo not
l~r th~ Ig~ bln~ to s~tur~ nd Ph~ p Ir~ ~o~ thlr lt
rollowu ~ut r oom~n~nt ~ I h~ ~lto~- that ~ ~ r o~
ld ntl~-l to tho-- of n~tur~ PhJ p II, ~ut ehoro 1- ~o untl~nlc
r-lut-dnu~- to natur~l or ~ollbln~nt aro~p ~ ll-rg
Flg. B h4W th hybrldl~-t~on o~ th~ o~hA oo~ or 7hl p ~ wlth
~rom tlsothy gr ~ ry~ ot~l ~A w~ ol-tod ~ron~ rn~
: ::: '; -~" ' ':
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5ENT BY:PENNIE ~ E~MONDS ;11-28-94; 3:52PM, PENNIE ~ EDM~NDSl PERLEY-RBBERTSDN;#l~
2~ 97~
nd tl~y gr~ ~na ~). IO ~ag wa~
ln denAtusing ~roae 9~ d blott~d ~to nitsoo~llulo~e. S~ 3~P-
l~l-d c~A, cotl~ fos P~l p II~ hybr~dLz-l~ wlth botll ry gr~
arld tlmothy gra~d Rrla ~t aE~l~t~ly th l~al of la9 ~ t ~ven
dl~tL~ly ~lo~ th~, ~t ~ ts~o~lpt ~ o~ ~bollt 60D b-s~
cro~ hy~rldlratlon ~how~ ~uctur~ il-rlty o~ th- ~orL~t~
vblA~h co~ ~or Oroup 21/III allganl- ln dlLf~rent 0s~-~ al~-a1o~-
P~ur~ A ~d 9 ~ 1~ th- ra~Lvlty o~ r~o~bLnant PA~ p IT
~11~ wth ~ ~ntibody ~oi~io to a-o~ II~lTl ~ .8~ pollen
A~ tll ~o~ uhloh ~ro~ tl~otlly ~ oll r~ ~llorg~8 ~d
tho ~irclp~ ~iroh t~ ,ll~g~ ot ~ Co~
n~r~bl~nt ~ t-~ted 1~ tha dot blot ~rooe-~ Uit~l
~nt~o~ ~olflo ~co gs -~ poll~ ll~e~. 4~ to.oro~p Y
llor~n-, R4 d t~- a~p ~ rpDs~ ~nd ~5 l~tlLlo~
~o~p II/llI ~ll-rg ~-
: , ~
1~ Th- dl~ lllu-tr t~ th- cl~ne d--Lg~4tlon ~ln nt
PhJ p 1~ 1~ ~rc~ y clot~ A.
~,v-le ~ th~ Phl n 11 ~
~he D~ no~ o~ the Phl p ~J, Uler~n w~l~ det~lrl d ~y ~oqu-noln~ ;
th oD~ ocord~g to th~ ~thod Or 8~gor ~16~ rlg~ u t~o
dotermln~d D~A -qu-no- ~nd th~ o ~cld e~ t duc-d t~rdro~
A 1CD~1 ~ ptSd in rrcnt o~ th- s~tur- prok-Ln~ ~bl4h poctLda how~
~lgnl~lo nt ho~olooy ~lth oth r uk~syot~o ign~l }-~tldo~
uno~ui~oa~lly provo- th~t Oroup Il/rI~ xga~o of dlotlnct g~c~
~r~g~t~ ~rn ood d ~nd do t ro~ult rro~ Osoup ~ ~ll~rg-~ throu~h
prot-olytlo d oo~po-ltlon Th~ hlgh d-~r-~ of -qu-n~- ho3Dlogy ~-~out ~ .
70~ ~quenco ld ntlty) of th- r~comblnu~t ~hl p Il wlth thD ~o~olo~o~
~rot-ln~ ~ro~ r~ gr~ oI p IT ~nd Lol p ~ r~. 2~ ohow~ th-
oloro ~tr~otur41 rd ~e~d~uo~ of tho~- protolna ~d th~rQby provlde- th
mol oul~r b~ or th ~munolo~ic~l rel~t-dno~ Or Oroup II/III
~ller~ ac Or ~ ront p ol~
: : :
SENT aY:PENN]E 8 EDMONDS ,11-2B-~4 : 3'52PM : PEN~IE 8 EDMONDSl PERLEY-RC3ERTSCN:#ll
.. . . . ..... . . . . . .. . .
2~ '.~ ~
D-terr~ of ~-o~ ~ T-cell~ ~
0~1 tha b~ o~ t~o dodYco~ ~no cld n~ o ~ tbo P~J1 p ~I ~llqr~-n
tb~ ~ell ~d ~-ccll ~topo~ ln d wlth ~1 ~d o~
ao~tcr ~r~- 124, 25)- Th- ~-lor~t ~oll uld ~-cell
~pLtopo~ r~ bwnp~l d Ln F~ 3 ~d ~- ~Iynthe~l~ pept~ whlCb
oorr-~pon4 to ~ plto~c, b~Lnd Igll ol c pOlloG-cllorglC
~tl-rlt~, Ybor~ ynth~t~o ~ptl,~l c~ ~1ch oo~apond to ~-oell
~lt;opo~ ot~t- ~-~llo o~ gr~o~ polloA~ o ~tLen~- to
prolLf-r~to~ u~d r ~ult Ln an l~r-~od ~-ehylD14in~ UpW~
~o~ln~nt ph~o- oon~lnl~ th cDN1~ Sor P~ .o Tl wore ~ ~-d for
l~-otlon ~Lth ly~og~lo t, ooll Y 1009. ~obl~nt ,O-gal~to-~da~
fu~ protoln w~ obt~ 4 by l~tlon ~1th IP~O lS~o;arapyl-
~
th~o l~oto~ uld oulturo ~0)~ ~rho control pro~ln p- --
ctw~ ho~ Io8 ~ludl~ ltl t~ ~-t~rn blot ~-~t~ wh-r-u~
th- r ~ln~t Phl p rI ~u~ pro'c-ln bo~ dl-t~ot Iq~ ~lndln~
~ol~lo~lly to ~ D Ip ol o tL~t- who r aot-d wlth Oroup ''fIlI
~11~A~ 0~ d~S-r, nt Cr~ pol~
~g~b~nd~o o~o ol~v S~ r ~P~ 12 IL A~r~
8-rum obt-~d rrO~ ~ gr~- pllon~ lo p~tllt Wh~ r-~ct~ w~th
n-tur~l ~roUp II~ r~, W~ pr-lncu~to~ wlth r~ bln~nt ~Al p
II, wh-r~ lsy arOu~ rT/rIl--p ol~lo Ig- w~- ~o~d rlg. 6 show- th-t th~ ,
blrldl~ to n~tt~r~l oroup I~J~ sgen~ ~oot co~pl-toly nnull-d
by th- prolnoub~tLon, ~ro-~ th~ blndln~ to Oroup I 4t Group V
llo~n~ w~ not .~r~t~. Thl- ~ h~t th~ lto~o~ o~ ~ur~
croup Il~III ~lorg ~ - blo~}od by ~omb~nt ~ TI, ~d th~t .
th r lu h~rtly ~ r l~ r~iv~ty b~w~A Crou~ I, Croup v : ;
~4 Oraup SI/IIS ~
-:, - -: ;~ -:
Q~o~- hvb~d~
~ot-l ~A rro~ rgo ~r~- ~a tl~othy ~r~-- P11-A W~ ol~
~t-d ln d~ urln~7 ~ ro~ ~1 ~d tr~r~ r~ onto nlt~-llulo~e - .: ~`
m~br no~. ~ho ~r~ hrbrldl~-d wlth ~ pl t- oDllA whlch ~o4~
9 '' ~
.-:: ~ .: ~
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:
SENT DY:PENNIE ~ EDMONDS ;11-2~-94 ; 3:53PM ; PENNIE ~ EDM~NDS- PERLEY-RO~ERTSON:#l~
21L~
For Pn~ p II. 2ybridlzlng blu~d~ ~r3 ~llw~y~ fo~nd ~t thN lo~el o~ th
1f~8 r~o~om~l F~A ~nd ~l~o ~onsid-r~ly balow th~t. ~t~ hybrldl~tlo~
1~ cl-~ly ~r- ln't-n~l /e wlth tlr~othy gr-o~ F~liA t~ Lt~ rye gs~-
~~, Although, ~oco~dl~ ~o the g 1 ~t~ ~g wlth ethldlun~ bro~
bollt ~ha o~ ~ou~t~ o~ to~ lll uor ~d ~e~rthe~ roo~
~rldl~tlos~. ce~uld b- utt~ a und~r ~tr~go~t ao~ld$tlon~ d~r
o~rt-ln c~-tu~ the l~-r tc~ orlp~ r~roo~nto L~rcl-r ~
~tlll ~turo ~ 04 th~ hybrldl,-atlorl with~t~d~ ~n 3trln~nt
wa-hl~. ~ lo ~t~d~ to do~t tho ~o109~ o~ t~o Oroul7
ll/I~r g~ao~ llorgon~ o~ dlff~nt ~ole-.
: :::~::.-
- :: : ~ : : : ~
"~.,'-' ~''."'',
::~;:
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SENT BY:PENNIE & EDMOHDS .11-2~-94 ; ~'53PM; PEI~INIE ~ EOM~NDSl PERLEY-RCBE~T30N;#13
.. ... . .. .. . . . . ~ .. .. . .. . . .. .
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WO 94123035 2.~ T9410003g
.
aibllog~aphy
1.1~reidl~off, ~.R., 3~hrlich ~tzky, E.,GI~lt, J~H., 1~syers, D.A~, ~nd
Manh, D.~. (19~ AUergy Cl~n Immunol 7~, 1190-1201
a. Matthlesen, ~., Lowensteil~, H. Clin 13xp Allergy 21, 309-320.
3. ~ns~ .A.,Shen~amtl~thi, P.,and l~a~sh, ~.~. (198g) J. Biol. Chem, :
264, 11181-11185
4. Kisil, F. T., Ja~ . S., Lin, Z. W., Ekranwdullah, A, X. M. (19g9) in
Sdwn ~ , D., Kunkcl, G. eds. Ep~topcs of Atopic Allcrgens, 22~
5. ~ei, K. S., ~kramDdullah, A. K. M., Kisil, P. T., ~zuba Pischcr, J. M. . ~ -;
M., Reclor, E. S., ~nt Sehon, A. El. (1989) J Allcrgy Clin Im~nuno183, 845-852.
5. v~n Rce, R., Dne~sen, ~. B. M., van Lc~uwen, W. A., Stapd, S. O., and .Aalbcrse, R. C. ~1992) Clin ~p t~iler~y.
7. Valenta, R" Puchene, M., Ebner, (:., Valent, P., Slllaber, C., Deviller,
P., ~errdra, P., Tejkl, M., Edelmann, H" Klaft, P., and Scheiner, O. (199~) J
8xp Med 17S, 377-38$. . . -
8. V~lenta, R., Dud~ene, M., Vrtala, S., Va~ent, P., Sillaber, C., Ferrdra,
F., l~kl, M., Hirschwehrl R., Ebner, C., Ksaft, 1:~., and Sch~iner, O. (1993) In¢ ~: -
Arch Allergy Appl Immunol 99, ~ 273.
9. Scheiner, O., Bohle, B., Broitenbach, M., Breitencd~r, H., Duch~ne, M.,
Ebner, C., Perrelra, F., Hirschwehr, ~, Hoffmann~ommergmber, K.,
Pettenburger, K., Rump~ld, H., Stciner, R., TejW;M., Valenh, R., and ~Craft, O. ~ `
tl992) in: Advanccs in Allergolo~y aod Clinical Immunology, Goda~t, P.,
~ousquet, ~., and ~Iichel, P. 13. eds. The Par~henon Publishing Group. .
10. Valen~a, R., Duchene, M., Vrtah, S., Birkner, T., Ebner, C.,
Hirschw~hr, R, Brcitenbach, M., Rumpcld, E~., Scheiner, O., and Kraft, D. (l991) --~
J Aller~y Clln Immunol ~8, 88g-8g4. . ` ~ ~-
11. Valenea, ~., Vrtala, S., Ebner, C., Kraft, D., and Schoinel, O. (1932) Int ~ ~ -
Arch Al~ergy Appl Immunol g7, 287-294
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12. Valenla, R., Spur, W. R., Per~e~rA, F, Valeflt, P., Sillaber, C., Tejkl,
M., Duch~nc, M., Ebner, C" Lechncr, ~., Kraft, D., and Scheincr, O. (1993). J
Allgy C1Jn ~mmunol 91, 88-91.
13. Vstal~, S, Spcrr, W. R., Ramittcr. I., Y~n~ec, R., Laffer, S., M~lbr,
W.-D., V~ , P., I.ochncr, K.l Rumpold, H., Kraf~, D., Scheiner, O" and
Y~Jenta, R. (1993) J Immunol (accepted proY~ded rwlsion), ~;
14. B~llened~r, H., Pcttenburser, K., Blto, A., Val~nta, ~.~ Kraft, 1).,
Rumpold, II., Scheincr, O., and Brciten~aeh, M. ~198g) El~BO J 8, 1935-19~8 .
15. Ausubel, P. M. tl990) in Current protocols in molecular biology, Wiley,
New York.
16. Sanger, ~., Ni~1den, S., Coulson, A. R, (lg77~ Proc Natl Acad Sci USA
74, 5463 5468.
t7. Vslenta, ~., Breilcneder, H., Pettenburger, K., Bre~tenbach, M.,
Rumpold, X., E~raft, D., and Schoiner, O. (l991) J Aller~y Clin Immunol 87, :~
677-682.
18. Sambrook,.~., Fritsch, E. P., Maniuis, T. (198g~ Molecular clonlng: a
Iaboratosy manu~l. Cold Sprin~ Harbor Laboratory Press.
19, Fdn~cr~, A. P., ant Vog~stein, B, (1983) ~nal Bioohcm 132, 6-13.
20. Huynh, T. V., Youn~, R. A., Davis, R. W. (1985) in: cl:~N~ clonin~
O~ord, ~L Press, v~ A7g~
21. Laemml; ~. ~C. (1970) Naturc 227, 680-685.
22 Towbin, H., Staehdin, T., Gordon, 1. (19i9~ Proc ~atl Acad Sci IJSA 76,
435W3~4. . :~
23. ~3a~el, Y., and He~ne, G. tl9~0) FEBS Lctt 261, 455-45~
24. Kyte, J. and Doolittle, R. F. (1982) J Mol Biol 157, 105-132.
2S, Margaiit, H., Spouge, J. L., Comette, J. L" Cease, K E~., Delisi, C., and ~-
~enofsky, J. A. (1987) J Immunol 138, 2213-2229.
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