Note: Descriptions are shown in the official language in which they were submitted.
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
New GP27-like polypeptides and DNA sequences thereof
Field of the Invention
This invention relates to newly identified polypeptides and
s polynucleotides encoding such polypeptides, to their use in diagnosis and
in identifying compounds that may be agonists, antagonists that are
potentially useful in therapy, and the production of such polypeptides and
polynucleotides, sometimes hereinafter referred to as HE8N124 belong to
the class of G-protein coupled receptors.
to Background of the Invention
The drug discovery process is currently undergoing a fundamental
revolution as it embraces "functional genomics", that is, high throughput
genome- or gene-based biology. This approach as a means to identify
genes and gene products as therapeutic targets is rapidly superceding
is earlier approaches based on "positional cloning". A phenotype, that is a
biological function or genetic disease, would be identified and this would
then be tracked back to the responsible gene, based on its genetic map
position.
Functional genomics relies heavily on high-throughput DNA sequencing
2o technologies and the various tools of bioinformatics to identify gene
sequences of potential interest from the many molecular biology databases
now available. There is a continuing need to identify and characterise
further genes and their related polypeptides/proteins, as targets for drug
discovery.
2s It is well established that many medically significant biological processes
are mediated by proteins participating in signal transduction pathways that
involve G-proteins and/or second messengers, e.g., cAMP (Lefkowitz,
Nature, 1991, 351:353-354). Herein these proteins are referred to as
proteins participating in pathways with G-proteins or PPG proteins. Some
3o examples of these proteins include the GPC receptors, such as those for
adrenergic agents and dopamine (Kobilka, B.K., et al., Proc. Natl Acad.
Sci., USA, 1987, 84:46-50; Kobilka, B.K., et al., Science, 1987, 238:650-
656; Bunzow, J.R., et al., Nature, 1988, 336:783-787), G-proteins
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
_2_
themselves, effector proteins, e.g., phospholipase C, adenyl cyclase, and
phosphodiesterase, and actuator proteins, e.g., protein kinase A and
protein kinase C (Simon, M.I., et al., Science, 1991, 252:802-8).
For example, in one form of signal transduction, the effect of hormone
s binding is activation of the enzyme, adenylate cyclase, inside the cell.
Enzyme activation by hormones is dependent on the presence of the
nucleotide GTP. GTP also influences hormone binding. A G-protein
connects the hormone receptor to adenylate cyclase. G-protein was shown
to exchange GTP for bound GDP when activated by a hormone receptor.
io The GTP-carrying form then binds to activated adenylate cyclase.
Hydrolysis of GTP to GDP, catalyzed by the G-protein itself, returns the G-
protein to its basal, inactive form. Thus, the G-protein serves a dual role,
as
an intermediate that relays the signal from receptor to effector, and as a
clock that controls the duration of the signal.
is The membrane protein gene superfamily of G-protein coupled receptors
has been characterized as having seven putative transmembrane domains.
The domains are believed to represent transmembrane a-helices
connected by extracellular or cytoplasmic loops. G-protein coupled
receptors include a wide range of biologically active receptors, such as
2o hormone, viral, growth factor and neuroreceptors.
G-protein coupled receptors (otherwise known as 7TM receptors) have
been characterized as including these seven conserved hydrophobic
stretches of about 20 to 30 amino acids, connecting at least eight divergent
hydrophilic loops. The G-protein family of coupled receptors includes
2s dopamine receptors which bind to neuroleptic drugs used for treating
psychotic and neurological disorders. Other examples of members of this
family include, but are not limited to, calcitonin, adrenergic, endothelia,
cAMP, adenosine, muscarinic, acetylcholine, serotonin, histamine,
thrombin, kinin, follicle stimulating hormone, opsins, endothelial
3o differentiation gene-1, rhodopsins, odorant, and cytomegalovirus receptors.
Most G-protein coupled receptors have single conserved cysteine residues
in each of the first two extracellular loops which form disulfide bonds that
are believed to stabilize functional protein structure. The 7 transmembrane
regions are designated as TM1, TM2, TM3, TM4, TMS, TM6, and TM7.
~s TM3 has been implicated in signal transduction.
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-3-
Phosphorylation and lipidation (palmitylation or farnesylation) of cysteine
residues can influence signal transduction of some G-protein coupled
receptors. Most G-protein coupled receptors contain potential
phosphorylation sites within the third cytoplasmic loop and/or the carboxy
s terminus. For several G-protein coupled receptors, such as the b-
adrenoreceptor, phosphorylation by protein kinase A and/or specific
receptor kinases mediates receptor desensitization.
For some receptors, the ligand binding sites of G-protein coupled receptors
are believed to comprise hydrophilic sockets formed by several G-protein
to coupled receptor transmembrane domains, said socket being surrounded
by hydrophobic residues of the G-protein coupled receptors. The
hydrophilic side of each G-protein coupled receptor transmembrane helix is
postulated to face inward and form polar ligand binding site. TM3 has been
implicated in several G-protein coupled receptors as having a ligand
is binding site, such as the TM3 aspartate residue. TM5 serines, a TM6
asparagine and TM6 or TM7 phenylalanines or tyrosines are also
implicated in ligand binding.
G-protein coupled receptors can be intracellularly coupled by heterotrimeric
G-proteins to various intracellular enzymes, ion channels and transporters
20 (see, Johnson et al., Endoc. Rev., 1989, 10:317-331 ) Different G-protein
a-subunits preferentially stimulate particular effectors to modulate various
biological functions in a cell. Phosphorylation of cytoplasmic residues of G-
protein coupled receptors have been identified as an important mechanism
for the regulation of G-protein coupling of some G-protein coupled
2s receptors. G-protein coupled receptors are found in numerous sites within
a mammalian host.
Over the past 15 years, nearly 350 therapeutic agents targeting 7
transmembrane (7 TM) receptors have been successfully introduced onto
the market.
3o Summary of the Invention
The present invention relates to HE8N124, in particular HE8N124
polypeptides and HE8N124 polynucleotides, recombinant materials and
methods for their production. Such polypeptides and polynucleotides are of
interest in relation to methods of treatment of certain diseases, including,
~s but not limited to, infections such as bacterial, fungal, protozoan and
viral
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-4-
infections, particularly infections caused by HIV-1 or HIV-2; pain; cancers;
diabetes, obesity; anorexia; bulimia; asthma; Parkinson's disease; acute
heart failure; hypotension; hypertension; urinary retention; osteoporosis;
angina pectoris; myocardial infarction; stroke; ulcers; asthma; allergies;
s benign prostatic hypertrophy; migraine; vomiting; psychotic and
neurological disorders, including anxiety, schizophrenia, manic depression,
depression, delirium, dementia, and severe mental retardation; and
dyskinesias, such as Huntington's disease or Gilles dela Tourett's
syndrome, hereinafter referred to as " diseases of the invention". In a
to further aspect, the invention relates to methods for identifying agonists
and antagonists (e.g., inhibitors) using the materials provided by the
invention, and treating conditions associated with HE8N124 imbalance
with the identified compounds. In a still further aspect, the invention
relates to diagnostic assays for detecting diseases associated with
Is inappropriate HE8N124 activity or levels.
Description of the Invention
In a first aspect, the present invention relates to HE8N124 polypeptides.
Such polypeptides include:
20 (a) an isolated polypeptide encoded by a polynucleotide comprising the
sequence of SEQ ID N0:1;
(b) an isolated polypeptide comprising a polypeptide sequence having at
least 95%, 96%, 97%, 98%, or 99% identity to the polypeptide sequence
of SEQ ID N0:2;
2s (c) an isolated polypeptide comprising the polypeptide sequence of SEQ
ID N0:2;
(d) an isolated polypeptide having at least 95%, 96%, 97%, 98%, or 99%
identity to the polypeptide sequence of SEQ ID N0:2;
(e) the polypeptide sequence of SEQ ID N0:2; and
~o (f) an isolated polypeptide having or comprising a polypeptide sequence
that has an Identity Index of 0.95, 0.96, 0.97, 0.98, or 0.99 compared to
the polypeptide sequence of SEQ ID N0:2;
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-s-
(g) fragments and variants of such polypeptides in (a) to (f).
Polypeptides of the present invention are believed to be members of the G
protein-coupled receptors family of polypeptides.
The biological properties of the HE8N124 are hereinafter referred to as
s "biological activity of HE8N124 or HE8N124 activity". Preferably, a
polypeptide of the present invention exhibits at least one biological
activity of HE8N124.
Polypeptides of the present invention also includes variants of the
aforementioned polypeptides, including all allelic forms and splice variants.
to Such polypeptides vary from the reference polypeptide by insertions,
deletions, and substitutions that may be conservative or non-conservative,
or any combination thereof. Particularly preferred variants are those in
which several, for instance from 50 to 30, from 30 to 20, from 20 to 10, from
to 5, from 5 to 3, from 3 to 2, from 2 to 1 or 1 amino acids are inserted,
Is substituted, or deleted, in any combination.
Preferred fragments of polypeptides of the present invention include an
isolated polypeptide comprising an amino acid sequence having at least
30, 50 or 100 contiguous amino acids from the amino acid sequence of
SEQ ID NO: 2, or an isolated polypeptide comprising an amino acid
2o sequence having at least 30, 50 or 100 contiguous amino acids truncated
or deleted from the amino acid sequence of SEQ ID NO: 2. Preferred
fragments are biologically active fragments that mediate the biological
activity of HE8N124, including those with a similar activity or an improved
activity, or with a decreased undesirable activity. Also preferred are those
2s fragments that are antigenic or immunogenic in an animal, especially in a
human.
Fragments of the polypeptides of the invention may be employed for
producing the corresponding full-length polypeptide by peptide synthesis;
therefore, these variants may be employed as intermediates for
producing the full-length polypeptides of the invention.The polypeptides of
the present invention may be in the form of the "mature" protein or may
be a part of a larger protein such as a precursor or a fusion protein. It is
often advantageous to include an additional amino acid sequence that
contains secretory or leader sequences, pro-sequences, sequences that
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-6-
aid in purification, for instance multiple histidine residues, or an
additional
sequence for stability during recombinant production.
Polypeptides of the present invention can be prepared in any suitable
manner, for instance by isolation form naturally occuring sources, from
s genetically engineered host cells comprising expression systems (vide
infra) or by chemical synthesis, using for instance automated peptide
synthesisers, or a combination of such methods.. Means for preparing
such polypeptides are well understood in the art.
to In a further aspect, the present invention relates to HE8N124
polynucleotides. Such polynucleotides include:
(a) an isolated polynucleotide comprising a polynucleotide sequence
having at least 95%, 96%, 97%, 98%, or 99% identity to the
polynucleotide squence of SEQ ID N0:1;
Is (b) an isolated polynucleotide comprising the polynucleotide of SEQ ID
N0:1;
(c) an isolated polynucleotide having at least 95%, 96%, 97%, 98%, or
99% identity to the polynucleotide of SEQ ID N0:1;
(d) the isolated polynucleotide of SEQ ID N0:1;
20 (e) an isolated polynucleotide comprising a polynucleotide sequence
encoding a polypeptide sequence having at least 95%, 96%, 97%, 98%, or
99% identity to the polypeptide sequence of SEQ ID N0:2;
(f) an isolated polynucleotide comprising a polynucleotide sequence
encoding the polypeptide of SEQ ID N0:2;
2s (g) an isolated polynucleotide having a polynucleotide sequence encoding
a polypeptide sequence having at least 95%, 96%, 97%, 98%, or 99%
identity to the polypeptide sequence of SEQ ID N0:2;
(h) an isolated polynucleotide encoding the polypeptide of SEQ ID N0:2;
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
(i) an isolated polynucleotide having or comprising a polynucleotide
sequence that has an Identity Index of 0.95, 0.96, 0.97, 0.98, or 0.99
compared to the polynucleotide sequence of SEQ ID N0:1;
(j) an isolated polynucleotide having or comprising a polynucleotide
s sequence encoding a polypeptide sequence that has an Identity Index of
0.95, 0.96, 0.97, 0.98, or 0.99 compared to the polypeptide sequence of
SEQ ID N0:2; and
polynucleotides that are fragments and variants of the above mentioned
polynucleotides or that are complementary to above mentioned
to polynucleotides, over the entire length thereof.
Preferred fragments of polynucleotides of the present invention include an
isolated polynucleotide comprising an nucleotide sequence having at least
15, 30, 50 or 100 contiguous nucleotides from the sequence of SEQ ID
NO: 1, or an isolated polynucleotide comprising an sequence having at
Is least 30, 50 or 100 contiguous nucleotides truncated or deleted from the
sequence of SEQ ID NO: 1.
Preferred variants of polynucleotides of the present invention include
splice variants, allelic variants, and polymorphisms, including
polynucleotides having one or more single nucleotide polymorphisms
20 (SNPs).
Polynucleotides of the present invention also include polynucleotides
encoding polypeptide variants that comprise the amino acid sequence of
SEQ ID N0:2 and in which several, for instance from 50 to 30, from 30 to
20, from 20 to 10, from 10 to 5, from 5 to 3, from 3 to 2, from 2 to 1 or 1
2s amino acid residues are substituted, deleted or added, in any combination.
In a further aspect, the present invention provides polynucleotides that
are RNA transcripts of the DNA sequences of the present invention.
Accordingly, there is provided an RNA polynucleotide that:
(a) comprises an RNA transcript of the DNA sequence encoding the
3o polypeptide of SEQ ID N0:2;
(b) is the RNA transcript of the DNA sequence encoding the polypeptide
of SEQ ID N0:2;
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
_g_
(c) comprises an RNA transcript of the DNA sequence of SEQ ID N0:1;
or
(d) is the RNA transcript of the DNA sequence of SEQ ID N0:1;
and RNA polynucleotides that are complementary thereto.
s
The polynucleotide sequence of SEQ ID N0:1 is a cDNA sequence that
encodes the polypeptide of SEQ ID N0:2. The polynucleotide sequence
encoding the polypeptide of SEQ ID N0:2 may be identical to the
polypeptide encoding sequence of SEQ ID N0:1 or it may be a
to sequence other than SEQ ID N0:1, which, as a result of the redundancy
(degeneracy) of the genetic code, also encodes the polypeptide of SEQ
ID N0:2. The polypeptide of the SEQ ID N0:2 is related to other proteins
of the G protein-coupled receptors family, having homology and/or
structural similarity with Gi-2739105 (Genomics 47 (2), 310-313; 1998)
is Preferred polypeptides and polynucleotides of the present invention are
expected to have, inter alia, similar biological functions/properties to their
homologous polypeptides and polynucleotides. Furthermore, preferred
polypeptides and polynucleotides of the present invention have at least one
H E8 N 124 activity.
2o Polynucleotides of the present invention may be obtained using standard
cloning and screening techniques from a cDNA library derived from mRNA
of human 8 week old whole embryo (see for instance, Sambrook et al.,
Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor
Laboratory Press, Cold Spring Harbor, N.Y. (1989)). Polynucleotides of
2s the invention can also be obtained from natural sources such as genomic
DNA libraries or can be synthesized using well known and commercially
available techniques.
When polynucleotides of the present invention are used for the
recombinant production of polypeptides of the present invention, the
3o polynucleotide may include the coding sequence for the mature
polypeptide, by itself, or the coding sequence for the mature polypeptide in
reading frame with other coding sequences, such as those encoding a
leader or secretory sequence, a pre-, or pro- or prepro- protein sequence,
or other fusion peptide portions. For example, a marker sequence that
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-9-
facilitates purification of the fused polypeptide can be encoded. In certain
preferred embodiments of this aspect of the invention, the marker sequence
is a hexa-histidine peptide, as provided in the pQE vector (Qiagen, Inc.)
and described in Gentz et al., Proc Natl Acad Sci USA (1989) 86:821-824,
s or is an HA tag. The polynucleotide may also contain non-coding 5' and 3'
sequences, such as transcribed, non-translated sequences, splicing and
polyadenylation signals, ribosome binding sites and sequences that
stabilize mRNA.
Polynucleotides that are identical, or have sufficient identity to a
to polynucleotide sequence of SEQ ID N0:1, may be used as hybridization
probes for cDNA and genomic DNA or as primers for a nucleic acid
amplification reaction (for instance, PCR). Such probes and primers may
be used to isolate full-length cDNAs and genomic clones encoding
polypeptides of the present invention and to isolate cDNA and genomic
is clones of other genes (including genes encoding paralogs from human
sources and orthologs and paralogs from species other than human) that
have a high sequence similarity to SEQ ID N0:1, typically at least 95%
identity. Preferred probes and primers will generally comprise at least 15
nucleotides, preferably, at least 30 nucleotides and may have at least 50, if
2o not at least 100 nucleotides. Particularly preferred probes will have
between 30 and 50 nucleotides. Particularly preferred primers will have
between 20 and 25 nucleotides.
A polynucleotide encoding a polypeptide of the present invention, including
homologs from species other than human, may be obtained by a process
2s comprising the steps of screening a library under stringent hybridization
conditions with a labeled probe having the sequence of SEQ ID NO: 1 or a
fragment thereof, preferably of at least 15 nucleotides; and isolating full-
length cDNA and genomic clones containing said polynucleotide sequence.
Such hybridization techniques are well known to the skilled artisan.
3o Preferred stringent hybridization conditions include overnight incubation
at
42oC in a solution comprising: 50% formamide, 5xSSC (150mM NaCI,
15mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5x Denhardt's
solution, 10 % dextran sulfate, and 20 microgram/ml denatured, sheared
salmon sperm DNA; followed by washing the filters in 0.1x SSC at about
3s 65oC. Thus the present invention also includes isolated polynucleotides.
preferably with a nucleotide sequence of at least 100, obtained by
screening a library under stringent hybridization conditions with a labeled
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-10-
probe having the sequence of SEQ ID N0:1 or a fragment thereof,
preferably of at least 15 nucleotides.
The skilled artisan will appreciate that, in many cases, an isolated cDNA
sequence will be incomplete, in that the region coding for the polypeptide
s does not extend all the way through to the 5' terminus. This is a
consequence of reverse transcriptase, an enzyme with inherently low
"processivity" (a measure of the ability of the enzyme to remain attached
to the template during the polymerisation reaction), failing to complete a
DNA copy of the mRNA template during first strand cDNA synthesis.
io There are several methods available and well known to those skilled in
the art to obtain full-length cDNAs, or extend short cDNAs, for example
those based on the method of Rapid Amplification of cDNA ends (RACE)
(see, for example, Frohman et al., Proc Nat Acad Sci USA 85, 8998-
9002, 1988). Recent modifications of the technique, exemplified by the
is Marathon (trade mark) technology (Clontech Laboratories Inc.) for
example, have significantly simplified the search for longer cDNAs. In the
Marathon (trade mark) technology, cDNAs have been prepared from
mRNA extracted from a chosen tissue and an 'adaptor' sequence ligated
onto each end. Nucleic acid amplification (PCR) is then carried out to
2o amplify the "missing" 5' end of the cDNA using a combination of gene
specific and adaptor specific oiigonucleotide primers. The PCR reaction
is then repeated using 'nested' primers, that is, primers designed to
anneal within the amplified product (typically an adaptor specific primer
that anneals further 3' in the adaptor sequence and a gene specific
2s primer that anneals further 5' in the known gene sequence). The
products of this reaction can then be analysed by DNA sequencing and a
full-length cDNA constructed either by joining the product directly to the
existing cDNA to give a complete sequence, or carrying out a separate
full-length PCR using the new sequence information for the design of the
~0 5' primer.
Recombinant polypeptides of the present invention may be prepared by
processes well known in the art from genetically engineered host cells
comprising expression systems. Accordingly, in a further aspect, the
present invention relates to expression systems comprising a
~s polynucleotide or polynucleotides of the present invention, to host cells
which are genetically engineered with such expression sytems and to the
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-11-
production of polypeptides of the invention by recombinant techniques.
Cell-free translation systems can also be employed to produce such
proteins using RNAs derived from the DNA constructs of the present
invention.
s For recombinant production, host cells can be genetically engineered to
incorporate expression systems or portions thereof for polynucleotides of
the present invention. Polynucleotides may be introduced into host cells by
methods described in many standard laboratory manuals, such as Davis et
al., Basic Methods in Molecular Biology (1986) and Sambrook et al.(ibid).
to Preferred methods of introducing polynucleotides into host cells include,
for
instance, calcium phosphate transfection, DEAE-dextran mediated
transfection, transvection, microinjection, cationic lipid-mediated
transfection, electroporation, transduction, scrape loading, ballistic
introduction or infection.
is Representative examples of appropriate hosts include bacterial cells, such
as Streptococci, Staphylococci, E. coli, Streptomyces and Bacillus subtilis
cells; fungal cells, such as yeast cells and Aspergillus cells; insect cells
such as Drosophila S2 and Spodoptera Sf9 cells; animal cells such as
CHO, COS, HeLa, C127, 3T3, BHK, HEK 293 and Bowes melanoma cells;
2o and plant cells.
A great variety of expression systems can be used, for instance,
chromosomal, episomal and virus-derived systems, e.g., vectors derived
from bacterial plasmids, from bacteriophage, from transposons, from yeast
episomes, from insertion elements, from yeast chromosomal elements,
2s from viruses such as baculoviruses, papova viruses, such as SV40,
vaccinia viruses, adenoviruses, fowl pox viruses, pseudorabies viruses and
retroviruses, and vectors derived from combinations thereof, such as those
derived from plasmid and bacteriophage genetic elements, such as
cosmids and phagemids. The expression systems may contain control
3o regions that regulate as well as engender expression. Generally, any
system or vector that is able to maintain, propagate or express a
polynucleotide to produce a polypeptide in a host may be used. The
appropriate polynucleotide sequence may be inserted into an expression
system by any of a variety of well-known and routine techniques, such as,
~s for example, those set forth in Sambrook et al., (ibid). Appropriate
secretion
signals may be incorporated into the desired polypeptide to allow secretion
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-12-
of the translated protein into the lumen of the endoplasmic reticulum, the
periplasmic space or the extracellular environment. These signals may be
endogenous to the polypeptide or they may be heterologous signals.
If a polypeptide of the present invention is to be expressed for use in
s screening assays, it is generally preferred that the polypeptide be
produced at the surface of the cell. In this event, the cells may be
harvested prior to use in the screening assay. If the polypeptide is
secreted into the medium, the medium can be recovered in order to
recover and purify the polypeptide. If produced intracellularly, the cells
to must first be lysed before the polypeptide is recovered.
Polypeptides of the present invention can be recovered and purified from
recombinant cell cultures by well-known methods including ammonium
sulfate or ethanol precipitation, acid extraction, anion or cation exchange
chromatography, phosphocellulose chromatography, hydrophobic
is interaction chromatography, affinity chromatography, hydroxylapatite
chromatography and lectin chromatography. Most preferably, high
performance liquid chromatography is employed for purification. Well
known techniques for refolding proteins may be employed to regenerate
active conformation when the polypeptide is denatured during intracellular
2o synthesis, isolation and/or purification.
Polynucleotides of the present invention may be used as diagnostic
reagents, through detecting mutations in the associated gene. Detection of
a mutated form of the gene characterised by the polynucleotide of SEQ ID
N0:1 in the cDNA or genomic sequence and which is associated with a
2s dysfunction will provide a diagnostic tool that can add to, or define, a
diagnosis of a disease, or susceptibility to a disease, which results from
under-expression, over-expression or altered spatial or temporal expression
of the gene. Individuals carrying mutations in the gene may be detected at
the DNA level by a variety of techniques well known in the art.
~o Nucleic acids for diagnosis may be obtained from a subject's cells, such as
from blood, urine, saliva, tissue biopsy or autopsy material. The genomic
DNA may be used directly for detection or it may be amplified enzymatically
by using PCR, preferably RT-PCR, or other amplification techniques prior to
analysis. RNA or cDNA may also be used in similar fashion. Deletions and
~s insertions can be detected by a change in size of the amplified product in
comparison to the normal genotype. Point mutations can be identified by
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-13-
hybridizing amplified DNA to labeled HE8N124 nucleotide sequences.
Perfectly matched sequences can be distinguished from mismatched
duplexes by RNase digestion or by differences in melting temperatures.
DNA sequence difference may also be detected by alterations in the
s electrophoretic mobility of DNA fragments in gels, with or without
denaturing agents, or by direct DNA sequencing (see, for instance, Myers
et al., Science (1985) 230:1242). Sequence changes at specific locations
may also be revealed by nuclease protection assays, such as RNase and
S1 protection or the chemical cleavage method (see Cotton et al., Proc Natl
to Acad Sci USA (1985) 85: 4397-4401).
An array of oligonucleotides probes comprising HE8N124 polynucleotide
sequence or fragments thereof can be constructed to conduct efficient
screening of e.g., genetic mutations. Such arrays are preferably high
density arrays or grids. Array technology methods are well known and
Is have general applicability and can be used to address a variety of
questions in molecular genetics including gene expression, genetic linkage,
and genetic variability, see, for example, M.Chee et al., Science, 274, 610-
613 (1996) and other references cited therein.
Detection of abnormally decreased or increased levels of polypeptide or
2o mRNA expression may also be used for diagnosing or determining
susceptibility of a subject to a disease of the invention. Decreased or
increased expression can be measured at the RNA level using any of the
methods well known in the art for the quantitation of polynucleotides,
such as, for example, nucleic acid amplification, for instance PCR, RT-
2s PCR, RNase protection, Northern blotting and other hybridization
methods. Assay techniques that can be used to determine levels of a
protein, such as a polypeptide of the present invention, in a sample derived
from a host are well-known to those of skill in the art. Such assay methods
include radioimmunoassays, competitive-binding assays, Western Blot
~o analysis and ELISA assays.
Thus in another aspect, the present invention relates to a diagonostic kit
comprising:
(a) a polynucleotide of the present invention, preferably the nucleotide
sequence of SEQ ID NO: 1, or a fragment or an RNA transcript thereof;
~s (b) a nucleotide sequence complementary to that of (a);
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-14-
(c) a polypeptide of the present invention, preferably the polypeptide of
SEQ ID N0:2 or a fragment thereof; or
(d) an antibody to a polypeptide of the present invention, preferably to the
polypeptide of SEQ ID N0:2.
s It will be appreciated that in any such kit, (a), (b), (c) or (d) may
comprise
a substantial component. Such a kit will be of use in diagnosing a
disease or susceptibility to a disease, particularly diseases of the
invention, amongst others.
The polynucleotide sequences of the present invention are valuable for
to chromosome localisation studies. The sequence is specifically targeted to,
and can hybridize with, a particular location on an individual human
chromosome. The mapping of relevant sequences to chromosomes
according to the present invention is an important first step in correlating
those sequences with gene associated disease. Once a sequence has
is been mapped to a precise chromosomal location, the physical position of
the sequence on the chromosome can be correlated with genetic map data.
Such data are found in, for example, V. McKusick, Mendelian Inheritance in
Man (available on-line through Johns Hopkins University Welch Medical
Library). The relationship between genes and diseases that have been
2o mapped to the same chromosomal region are then identified through
linkage analysis (co-inheritance of physically adjacent genes). Precise
human chromosomal localisations for a genomic sequence (gene
fragment etc.) can be determined using Radiation Hybrid (RH) Mapping
(Walter, M. Spillett, D., Thomas, P., Weissenbach, J., and Goodfellow, P.,
2s (1994) A method for constructing radiation hybrid maps of whole
genomes, Nature Genetics 7, 22-28). A number of RH panels are
available from Research Genetics (Huntsville, AL, USA) e.g. the
GeneBridge4 RH panel (Hum Mol Genet 1996 Mar;S(3):339-46 P,
radiation hybrid map of the human genome. Gyapay G, Schmitt K,
3o Fizames C, Jones H, Vega-Czarny N, Spillett D, Muselet D, Prud'Homme
JF, Dib C, Auffray C, Morissette J, Weissenbach J, Goodfellow PN). To
determine the chromosomal location of a gene using this panel, 93 PCRs
are performed using primers designed from the gene of interest on RH
DNAs. Each of these DNAs contains random human genomic fragments
~s maintained in a hamster background (human / hamster hybrid cell lines).
These PCRs result in 93 scores indicating the presence or absence of
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-15-
the PCR product of the gene of interest. These scores are compared
with scores created using PCR products from genomic sequences of
known location. This comparison is conducted at
http://www.genome.wi.mit.edu/.
s The polynucleotide sequences of the present invention are also valuable
tools for tissue expression studies. Such studies allow the determination of
expression patterns of polynucleotides of the present invention which may
give an indication as to the expression patterns of the encoded
polypeptides in tissues, by detecting the mRNAs that encode them. The
io techniques used are well known in the art and include in situ hydridisation
techniques to clones arrayed on a grid, such as cDNA microarray
hybridisation (Schena et al, Science, 270, 467-470, 1995 and Shalon et al,
Genome Res, 6, 639-645, 1996) and nucleotide amplification techniques
such as PCR. A preferred method uses the TAQMAN (Trade mark)
is technology available from Perkin Elmer. Results from these studies can
provide an indication of the normal function of the polypeptide in the
organism. In addition, comparative studies of the normal expression
pattern of mRNAs with that of mRNAs encoded by an alternative form of
the same gene (for example, one having an alteration in polypeptide coding
2o potential or a regulatory mutation) can provide valuable insights into the
role
of the polypeptides of the present invention, or that of inappropriate
expression thereof in disease. Such inappropriate expression may be of a
temporal, spatial or simply quantitative nature.
The polypeptides of the present invention are expressed in human fetal
2s heart, ovarian tumour, infant brain, embryo and testis
A further aspect of the present invention relates to antibodies. The
polypeptides of the invention or their fragments, or cells expressing them,
can be used as immunogens to produce antibodies that are immunospecific
for polypeptides of the present invention. The term "immunospecific"
~o means that the antibodies have substantially greater affinity for the
polypeptides of the invention than their affinity for other related
polypeptides
in the prior art.
Antibodies generated against polypeptides of the present invention may be
obtained by administering the polypeptides or epitope-bearing fragments, or
~s cells to an animal, preferably a non-human animal, using routine protocols.
For preparation of monoclonal antibodies, any technique which provides
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-16-
antibodies produced by continuous cell line cultures can be used.
Examples include the hybridoma technique (Kohler, G. and Milstein, C.,
Nature (1975) 256:495-4.97), the trioma technique, the human B-cell
hybridoma technique (Kozbor et aL, Immunology Today (1983) 4:72) and
s the EBV-hybridoma technique (Cole et aL, Monoclonal Antibodies and
Cancer Therapy, 77-96, Alan R. Liss, Inc., 1985).
Techniques for the production of single chain antibodies, such as those
described in U.S. Patent No. 4,946,778, can also be adapted to produce
single chain antibodies to polypeptides of this invention. Also, transgenic
to mice, or other organisms, including other mammals, may be used to
express humanized antibodies.
The above-described antibodies may be employed to isolate or to identify
clones expressing the polypeptide or to purify the polypeptides by affinity
chromatography. Antibodies against polypeptides of the present invention
Is may also be employed to treat diseases of the invention, amongst others.
Polypeptides and polynucleotides of the present invention may also be
used as vaccines. Accordingly, in a further aspect, the present invention
relates to a method for inducing an immunological response in a mammal
that comprises inoculating the mammal with a polypeptide of the present
2o invention, adequate to produce antibody and/or T cell immune response,
including, for example, cytokine-producing T cells or cytotoxic T cells, to
protect said animal from disease, whether that disease is already
established within the individual or not. An immunological response in a
mammal may also be induced by a method comprises delivering a
?s polypeptide of the present invention via a vector directing expression of
the polynucleotide and coding for the polypeptide in vivo in order to
induce such an immunological response to produce antibody to protect
said animal from diseases of the invention. One way of administering the
vector is by accelerating it into the desired cells as a coating on particles
30 or otherwise. Such nucleic acid vector may comprise DNA, RNA, a
modified nucleic acid, or a DNA/RNA hybrid. For use a vaccine, a
polypeptide or a nucleic acid vector will be normally provided as a
vaccine formulation (composition). The formulation may further comprise
a suitable carrier. Since a polypeptide may be broken down in the
~s stomach, it is preferably administered parenterally (for instance,
subcutaneous, intramuscular, intravenous, or intradermal injection).
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-17-
Formulations suitable for parenteral administration include aqueous and
non-aqueous sterile injection solutions that may contain anti-oxidants,
buffers, bacteriostats and solutes that render the formulation instonic with
the blood of the recipient; and aqueous and non-aqueous sterile
s suspensions that may include suspending agents or thickening agents.
The formulations may be presented in unit-dose or multi-dose containers,
for example, sealed ampoules and vials and may be stored in a freeze-
dried condition requiring only the addition of the sterile liquid carrier
immediately prior to use. The vaccine formulation may also include
to adjuvant systems. for enhancing the immunogenicity of the formulation,
such as oil-in water systems and other systems known in the art. The
dosage will depend on the specific activity of the vaccine and can be
readily determined by routine experimentation.
Polypeptides of the present invention have one or more biological functions
is that are of relevance in one or more disease states, in particular the
diseases of the invention hereinbefore mentioned. It is therefore useful to
to identify compounds that stimulate or inhibit the function or level of the
polypeptide. Accordingly, in a further aspect, the present invention
provides for a method of screening compounds to identify those that
2o stimulate or inhibit the function or level of the polypeptide. Such methods
identify agonists or antagonists that may be employed for therapeutic and
prophylactic purposes for such diseases of the invention as hereinbefore
mentioned. Compounds may be identified from a variety of sources, for
example, cells, cell-free preparations, chemical libraries, collections of
2s chemical compounds, and natural product mixtures. Such agonists or
antagonists so-identified may be natural or modified substrates, ligands,
receptors, enzymes, etc., as the case may be, of the polypeptide; a
structural or functional mimetic thereof (see Coligan et al., Current
Protocols in Immunology 1 (2):Chapter 5 (1991 )) or a small molecule.
3o The screening method may simply measure the binding of a candidate
compound to the polypeptide, or to cells or membranes bearing the
polypeptide, or a fusion protein thereof, by means of a label directly or
indirectly associated with the candidate compound. Alternatively, the
screening method may involve measuring or detecting (qualitatively or
3s quantitatively) the competitive binding of a candidate compound to the
polypeptide against a labeled competitor (e.g. agonist or antagonist).
Further, these screening methods may test whether the candidate
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
_18_
compound results in a signal generated by activation or inhibition of the
polypeptide, using detection systems appropriate to the cells bearing the
polypeptide. Inhibitors of activation are generally assayed in the
presence of a known agonist and the effect on activation by the agonist
s by the presence of the candidate compound is observed. Further, the
screening methods may simply comprise the steps of mixing a candidate
compound with a solution containing a polypeptide of the present
invention, to form a mixture, measuring a HE8N124 activity in the mixture,
and comparing the HE8N124 activity of the mixture to a control mixture
io which contains no candidate compound.
Polypeptides of the present invention may be employed in conventional
low capacity screening methods and also in high-throughput screening
(HTS) formats. Such HTS formats include not only the well-established
use of 96- and, more recently, 384-well micotiter plates but also emerging
Is methods such as the nanowell method described by Schullek et al, Anal
Biochem., 246, 20-29, (1997).
Fusion proteins, such as those made from Fc portion and HE8N124
polypeptide, as hereinbefore described, can also be used for
high-throughput screening assays to identify antagonists for the
2o polypeptide of the present invention (see D. Bennett et al., J Mol
Recognition, 8:52-58 (1995); and K. Johanson et al., J Biol Chem,
270(16):9459-9471 (1995)).
One screening technique includes the use of cells which express receptor
of this invention (for example, transfected CHO cells) in a system which
2s measures extracellular pH or intracellular calcium changes caused by
receptor activation. In this technique, compounds may be contacted with
cells expressing the receptor polypeptide of the present invention. A
second messenger response, e.g., signal transduction, pH changes, or
changes in calcium level, is then measured to determine whether the
3o potential compound activates or inhibits the receptor.
Another method involves screening for receptor inhibitors by determining
inhibition or stimulation of receptor-mediated cAMP and/or adenylate
cyclase accumulation. Such a method involves transfecting a eukaryotic
cell with the receptor of this invention to express the receptor on the cell
~s surface. The cell is then exposed to potential antagonists in the presence
of the receptor of this invention. The amount of CAMP accumulation is then
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-19-
measured. If the potential antagonist binds the receptor, and thus inhibits
receptor binding, the levels of receptor-mediated CAMP, or adenylate
cyclase, activity will be reduced or increased.
Another methods for detecting agonists or antagonists for the receptor of
s the present invention is the yeast based technology as described in U.S.
Patent 5,482,835.
Screening techniques
The polynucleotides, polypeptides and antibodies to the polypeptide of the
to present invention may also be used to configure screening methods for
detecting the effect of added compounds on the production of mRNA and
polypeptide in cells. For example, an ELISA assay may be constructed
for measuring secreted or cell associated levels of polypeptide using
monoclonal and polyclonal antibodies by standard methods known in the
is art. This can be used to discover agents that may inhibit or enhance the
production of polypeptide (also called antagonist or agonist, respectively)
from suitably manipulated cells or tissues.
A polypeptide of the present invention may be used to identify membrane
bound or soluble receptors, if any, through standard receptor binding
2o techniques known in the art. These include, but are not limited to, ligand
binding and crosslinking assays in which the polypeptide is labeled with a
radioactive isotope (for instance, X251), chemically modified (for instance,
biotinylated), or fused to a peptide sequence suitable for detection or
purification, and incubated with a source of the putative receptor (cells,
2s cell membranes, cell supernatants, tissue extracts, bodily fluids). Other
methods include biophysical techniques such as surface plasmon
resonance and spectroscopy. These screening methods may also be
used to identify agonists and antagonists of the polypeptide that compete
with the binding of the polypeptide to its receptors, if any. Standard
3o methods for conducting such assays are well understood in the art.
Examples of antagonists of polypeptides of the present invention include
antibodies or, in some cases, oligonucleotides or proteins that are closely
related to the ligands, substrates, receptors, enzymes, etc., as the case
may be, of the polypeptide, e.g., a fragment of the ligands, substrates,
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
20-
receptors, enzymes, etc.; or a small molecule that bind to the polypeptide of
the present invention but do not elicit a response, so that the activity of
the
polypeptide is prevented.
Screening methods may also involve the use of transgenic technology
s and HE8N124 gene. The art of constructing transgenic animals is well
established. For example, the HE8N124 gene may be introduced through
microinjection into the male pronucleus of fertilized oocytes, retroviral
transfer into pre- or post-implantation embryos, or injection of genetically
modified, such as by electroporation, embryonic stem cells into host
to blastocysts. Particularly useful transgenic animals are so-called "knock-
in" animals in which an animal gene is replaced by the human equivalent
within the genome of that animal. Knock-in transgenic animals are useful
in the drug discovery process, for target validation, where the compound
is specific for the human target. Other useful transgenic animals are so-
ts called "knock-out" animals in which the expression of the animal ortholog
of a polypeptide of the present invention and encoded by an endogenous
DNA sequence in a cell is partially or completely annulled. The gene
knock-out may be targeted to specific cells or tissues, may occur only in
certain cells or tissues as a consequence of the limitations of the
2o technology, or may occur in all, or substantially all, cells in the animal.
Transgenic animal technology also offers a whole animal expression-
cloning system in which introduced genes are expressed to give large
amounts of polypeptides of the present invention
Screening kits for use in the above described methods form a further
2s aspect of the present invention. Such screening kits comprise:
(a) a polypeptide of the present invention;
(b) a recombinant cell expressing a polypeptide of the present invention;
(c) a cell membrane expressing a polypeptide of the present invention; or
(d) an antibody to a polypeptide of the present invention;
3o which polypeptide is preferably that of SEQ ID N0:2.
It will be appreciated that in any such kit, (a), (b), (c) or (d) may comprise
a substantial component.
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-21-
Glossary
The following definitions are provided to facilitate understanding of certain
terms used frequently hereinbefore.
s "Antibodies" as used herein includes polyclonal and monoclonal
antibodies, chimeric, single chain, and humanized antibodies, as well as
Fab fragments, including the products of an
Fab or other immunoglobulin expression library.
"Isolated" means altered "by the hand of man" from its natural state, i.e.,
io if it occurs in nature, it has been changed or removed from its original
environment, or both. For example, a polynucleotide or a polypeptide
naturally present in a living organism is not "isolated," but the same
polynucleotide or polypeptide separated from the coexisting materials of
its natural state is "isolated", as the term is employed herein. Moreover,
is a polynucleotide or polypeptide that is introduced into an organism by
transformation, genetic manipulation or by any other recombinant method
is "isolated" even if it is still present in said organism, which organism
may be living or non-living.
"Polynucleotide" generally refers to any polyribonucleotide (RNA) or
2o polydeoxribonucleotide (DNA), which may be unmodified or modified
RNA or DNA. "Polynucleotides" include, without limitation, single- and
double-stranded DNA, DNA that is a mixture of single- and double-
stranded regions, single- and double-stranded RNA, and RNA that is
mixture of single- and double-stranded regions, hybrid molecules
2s comprising DNA and RNA that may be single-stranded or, more typically,
double-stranded or a mixture of single- and double-stranded regions. 1r
addition, "polynucleotide" refers to triple-stranded regions comprising
RNA or DNA or both RNA and DNA. The term "polynucleotide" also
includes DNAs or RNAs containing one or more modified bases and
3o DNAs or RNAs with backbones modified for stability or for other reasons.
"Modified" bases include, for example, tritylated bases and unusual bases
such as inosine. A variety of modifications may be made to DNA and
RNA; thus, "polynucleotide" embraces chemically, enzymatically or
metabolically modified forms of polynucleotides as typically found in
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
22-
nature, as well as the chemical forms of DNA and RNA characteristic of
viruses and cells. "Polynucleotide" also embraces relatively short
polynucleotides, often referred to as oligonucleotides.
"Polypeptide" refers to any polypeptide comprising two or more amino
s acids joined to each other by peptide bonds or modified peptide bonds,
i.e., peptide isosteres. "Polypeptide" refers to both short chains,
commonly referred to as peptides, oligopeptides or oligomers, and to
longer chains, generally referred to as proteins. Polypeptides may
contain amino acids other than the 20 gene-encoded amino acids.
to "Polypeptides" include amino acid sequences modified either by natural
processes, such as post-translational processing, or by chemical
modification techniques that are well known in the art. Such
modifications are well described in basic texts and in more detailed
monographs, as well as in a voluminous research literature.
is Modifications may occur anywhere in a polypeptide, including the peptide
backbone, the amino acid side-chains and the amino or carboxyl termini.
It will be appreciated that the same type of modification may be present
to the same or varying degrees at several sites in a given polypeptide.
Also, a given polypeptide may contain many types of modifications.
2o Polypeptides may be branched as a result of ubiquitination, and they may
be cyclic, with or without branching. Cyclic, branched and branched
cyclic polypeptides may result from post-translation natural processes or
may be made by synthetic methods. Modifications include acetylation,
acylation, ADP-ribosylation, amidation, biotinylation, covalent attachment
2s of flavin, covalent attachment of a heme moiety, covalent attachment of a
nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid
derivative, covalent attachment of phosphotidylinositol, cross-linking,
cyclization, disulfide bond formation, demethylation, formation of covalent
cross-links, formation of cystine, formation of pyroglutamate, formylation,
~o gamma-carboxylation, glycosylation, GPI anchor formation,
hydroxylation, iodination, methylation, myristoylation, oxidation,
proteolytic processing, phosphorylation, prenylation, racemization,
selenoylation, sulfation, transfer-RNA mediated addition of amino acids to
proteins such as arginylation, and ubiquitination (see, for instance,
~s Proteins - Structure and Molecular Properties, 2nd Ed., T. E. Creighton,
W. H. Freeman and Company, New York, 1993; Wold, F., Post-
translational Protein Modifications: Perspectives and Prospects, 1-12, in
Post-translational Covalent Modification of Proteins, B. C. Johnson, Ed.,
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-23-
Academic Press, New York, 1983; Seifter et al., "Analysis for protein
modifications and nonprotein cofactors", Meth Enzymol, 182, 626-646,
1990, and Rattan et al., "Protein Synthesis: Post-translational
Modifications and Aging", Ann NY Acad Sci, 663, 48-62, 1992).
s "Fragment" of a polypeptide sequence refers to a polypeptide sequence
that is shorter than the reference sequence but that retains essentially the
same biological function or activity as the reference polypeptide.
"Fragment" of a polynucleotide sequence refers to a polynucloetide
sequence that is shorter than the reference sequence of SEQ ID N0:1..
to "Variant" refers to a polynucleotide or polypeptide that differs from a
reference polynucleotide or polypeptide, but retains the essential
properties thereof. A typical variant of a polynucleotide differs in
nucleotide sequence from the reference polynucleotide. Changes in the
nucleotide sequence of the variant may or may not alter the amino acid
is sequence of a polypeptide encoded by the reference polynucleotide.
Nucleotide changes may result in amino acid substitutions, additions,
deletions, fusions and truncations in the polypeptide encoded by the
reference sequence, as discussed below. A typical variant of a
polypeptide differs in amino acid sequence from the reference
2o polypeptide. Generally, alterations are limited so that the sequences of
the reference polypeptide and the variant are closely similar overall and,
in many regions, identical. A variant and reference polypeptide may differ
in amino acid sequence by one or more substitutions, insertions,
deletions in any combination. A substituted or inserted amino acid
2s residue may or may not be one encoded by the genetic code. Typical
conservative substitutions include Gly, Ala; Val, Ile, Leu; Asp, Glu; Asn,
Gln;
Ser, Thr; Lys, Arg; and Phe and Tyr. A variant of a polynucleotide or
polypeptide may be naturally occurring such as an allele, or it may be a
variant that is not known to occur naturally. Non-naturally occurring
3o variants of polynucleotides and polypeptides may be made by
mutagenesis techniques or by direct synthesis. Also included as variants
are polypeptides having one or more post-translational modifications, for
instance glycosylation, phosphorylation, methylation, ADP ribosylation
and the like. Embodiments include methylation of the N-terminal amino
3s acid, phosphorylations of serines and threonines and modification of C-
terminal glycines.
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
24-
"Allele" refers to one of two or more alternative forms of a gene occuring
at a given locus in the genome.
"Polymorphism" refers to a variation in nucleotide sequence (and
encoded polypeptide sequence, if relevant) at a given position in the
s genome within a population.
"Single Nucleotide Polymorphism" (SNP) refers to the occurence of
nucleotide variability at a single nucleotide position in the genome, within
a population. An SNP may occur within a gene or within intergenic
regions of the genome. SNPs can be assayed using Allele Specific
to Amplification (ASA). For the process at least 3 primers are required. A
common primer is used in reverse complement to the polymorphism
being assayed. This common primer can be between 50 and 1500 bps
from the polymorphic base. The other two (or more) primers are identical
to each other except that the final 3' base wobbles to match one of the
is two (or more) alleles that make up the polymorphism. Two (or more)
PCR reactions are then conducted on sample DNA, each using the
common primer and one of the Allele Specific Primers.
"Splice Variant" as used herein refers to cDNA molecules produced from
RNA molecules initially transcribed from the same genomic DNA
2o sequence but which have undergone alternative RNA splicing.
Alternative RNA splicing occurs when a primary RNA transcript
undergoes splicing, generally for the removal of introns, which results in
the production of more than one mRNA molecule each of that may
encode different amino acid sequences. The term splice variant also
2s refers to the proteins encoded by the above cDNA molecules.
"Identity" reflects a relationship between two or more polypeptide
sequences or two or more polynucleotide sequences, determined by
comparing the sequences. In general, identity refers to an exact
nucleotide to nucleotide or amino acid to amino acid correspondence of
~o the two polynucleotide or two polypeptide sequences, respectively, over
the length of the sequences being compared.
"% Identity" - For sequences where there is not an exact
correspondence, a "% identity" may be determined. In general, the two
sequences to be compared are aligned to give a maximum correlation
~s between the sequences. This may include inserting "gaps" in either one
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-25-
or both sequences, to enhance the degree of alignment. A % identity
may be determined over the whole length of each of the sequences being
compared (so-called global alignment), that is particularly suitable for
sequences of the same or very similar length, or over shorter, defined
s lengths (so-called local alignment), that is more suitable for sequences of
unequal length.
"Similarity" is a further, more sophisticated measure of the relationship
between two polypeptide sequences. In general, "similarity" means a
comparison between the amino acids of two polypeptide chains, on a
to residue by residue basis, taking into account not only exact
correspondences between a between pairs of residues, one from each of
the sequences being compared (as for identity) but also, where there is
not an exact correspondence, whether, on an evolutionary basis, one
residue is a likely substitute for the other. This likelihood has an
is associated "score" from which the "% similarity" of the two sequences
can then be determined.
Methods for comparing the identity and similarity of two or more
sequences are well known in the art. Thus for instance, programs
available in the Wisconsin Sequence Analysis Package, version 9.1
20 (Devereux J et al, Nucleic Acids Res, 12, 387-395, 1984, available from
Genetics Computer Group, Madison, Wisconsin, USA), for example the
programs BESTFIT and GAP, may be used to determine the % identity
between two polynucleotides and the % identity and the % similarity
between two polypeptide sequences. BESTFIT uses the "local
2s homology" algorithm of Smith and Waterman (J Mol Biol, 147,195-197,
1981, Advances in Applied Mathematics, 2, 482-489, 1981 ) and finds the
best single region of similarity between two sequences. BESTFIT is
more suited to comparing two polynucleotide or two polypeptide
sequences that are dissimilar in length, the program assuming that the
~o shorter sequence represents a portion of the longer. In comparison, GAP
aligns two sequences, finding a "maximum similarity", according to the
algorithm of Neddleman and Wunsch (J Mol Biol, 48, 443-453, 1970).
GAP is more suited to comparing sequences that are approximately the
same length and an alignment is expected over the entire length.
~s Preferably, the parameters "Gap Weight" and "Length Weight" used in
each program are 50 and 3, for polynucleotide sequences and 12 and 4
for polypeptide sequences, respectively. Preferably, % identities and
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-26-
similarities are determined when the two sequences being compared are
optimally aligned.
Other programs for determining identity and/or similarity between
sequences are also known in the art, for instance the BLAST family of
s programs (Altschul S F et al, J Mol Biol, 215, 403-410, 1990, Altschul S F
et al, Nucleic Acids Res., 25:389-3402, 1997, available from the National
Center for Biotechnology Information (NCBI), Bethesda, Maryland, USA
and accessible through the home page of the NCBI at
www.ncbi.nlm.nih.gov) and FASTA (Pearson W R, Methods in
to Enzymology, 183, 63-99, 1990; Pearson W R and Lipman D J, Proc Nat
Acad Sci USA, 85, 2444-2448,1988, available as part of the Wisconsin
Sequence Analysis Package).
Preferably, the BLOSUM62 amino acid substitution matrix (Henikoff S
and Henikoff J G, Proc. Nat. Acad Sci. USA, 89, 10915-10919, 1992) is
is used in polypeptide sequence comparisons including where nucleotide
sequences are first translated into amino acid sequences before
comparison.
Preferably, the program BESTFIT is used to determine the % identity of a
query polynucleotide or a polypeptide sequence with respect to a
?o reference polynucleotide or a polypeptide sequence, the query and the
reference sequence being optimally aligned and the parameters of the
program set at the default value, as hereinbefore described.
"Identity Index" is a measure of sequence relatedness which may be
used to compare a candidate sequence (polynucleotide or polypeptidej
?s and a reference sequence. Thus, for instance, a candidate
polynucleotide sequence having, for example, an Identity Index of 0.95
compared to a reference polynucleotide sequence is identical to the
reference sequence except that the candidate polynucleotide sequence
may include on average up to five differences per each 100 nucleotides
~o of the reference sequence. Such differences are selected from the group
consisting of at least one nucleotide deletion, substitution, including
transition and transversion, or insertion. These differences may occur at
~s
the 5' or 3' terminal positions of the reference polynucleotide sequence or
anywhere between these terminal positions, interspersed either
individually among the nucleotides in the reference sequence or in one or
more contiguous groups within the reference sequence. In other words,
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-27-
to obtain a polynucleotide sequence having an Identity Index of 0.95
compared to a reference polynucleotide sequence, an average of up to 5
in every 100 of the nucleotides of the in the reference sequence may be
deleted, substituted or inserted, or any combination thereof, as
s hereinbefore described. The same applies mutatis mutandis for other
values of the Identity Index, for instance 0.96, 0.97, 0.98 and 0.99.
Similarly, for a polypeptide, a candidate polypeptide sequence having, for
example, an Identity Index of 0.95 compared to a reference polypeptide
sequence is identical to the reference sequence except that the
to polypeptide sequence may include an average of up to five differences
per each 100 amino acids of the reference sequence. Such differences
are selected from the group consisting of at least one amino acid
deletion, substitution, including conservative and non-conservative
substitution, or insertion. These differences may occur at the amino- or
Is carboxy-terminal positions of the reference polypeptide sequence or
anywhere between these terminal positions, interspersed either
individually among the amino acids in the reference sequence or in one
or more contiguous groups within the reference sequence. In other
words, to obtain a polypeptide sequence having an Identity Index of 0.95
2o compared to a reference polypeptide sequence, an average of up to 5 in
every 100 of the amino acids in the reference sequence may be deleted,
substituted or inserted, or any combination thereof, as hereinbefore
described. The same applies mutafis mutandis for other values of the
Identity Index, for instance 0.96, 0.97, 0.98 and 0.99.
2s The relationship between the number of nucleotide or amino acid
differences and the Identity Index may be expressed in the following
equation:
na ~ xa - (xa ~ I),
in which:
~o na is the number of nucleotide or amino acid differences,
xa is the total number of nucleotides or amino acids in SEQ ID N0:1 or
SEQ ID N0:2, respectively,
I is the Identity Index ,
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
- 28 -
~ is the symbol for the multiplication operator, and
in which any non-integer product of xa and I is rounded down to the
nearest integer prior to subtracting it from xa.
"Homolog" is a generic term used in the art to indicate a polynucleotide or
s polypeptide sequence possessing a high degree of sequence relatedness
to a reference sequence. Such relatedness may be quantified by
determining the degree of identity and/or similarity between the two
sequences as hereinbefore defined. Falling within this generic term are
the terms "ortholog", and "paralog". "Ortholog" refers to a polynucleotide
to or polypeptide that is the functional equivalent of the polynucleotide or
polypeptide in another species. "Paralog" refers to a polynucleotideor
polypeptide that within the same species which is functionally similar.
"Fusion protein" refers to a protein encoded by two, unrelated, fused
genes or fragments thereof. Examples have been disclosed in US
is 5541087, 5726044. In the case of Fc-HE8N124, employing an
immunoglobulin Fc region as a part of a fusion protein is advantageous
for performing the functional expression of Fc-HE8N124 or fragments of
HE8N124, to improve pharmacokinetic properties of such a fusion protein
when used for therapy and to generate a dimeric Fc-HE8N124 . The Fc-
2o HE8N124 DNA construct comprises in 5' to 3' direction, a secretion
cassette, i.e. a signal sequence that triggers export from a mammalian
cell, DNA encoding an immunoglobulin Fc region fragment, as a fusion
partner, and a DNA encoding Fc-HE8N124 or fragments thereof. In some
uses it would be desirable to be able to alter the intrinsic functional
2s properties (complement binding, Fc-Receptor binding) by mutating the
functional Fc sides while leaving the rest of the fusion protein untouched
or delete the Fc part completely after expression.
All publications and references, including but not limited to patents and
~o patent applications, cited in this specification are herein incorporated by
reference in their entirety as if each individual publication or reference
were specifically and individually indicated to be incorporated by
reference herein as being fully set forth. Any patent application to which
this application claims priority is also incorporated by reference herein in
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
- 29 -
its entirety in the manner described above for publications and
references.
Figure 1: Analysis of the expression of gp271ike polypeptide mRNA in
human tissues. GP-271ike protein is expressed in various human tissues
s and organes. The labeling of bars in Figure 3 is as follows:
1. whole brain 2. cerebral cortex3. frontal lobe
4. parietal lobe 5. occipital lobe 6. temporal lobe
7. paracentral 8. pons 9. cerebellum left
gyrus
10. cerebellum 11. corpus callosum12. amygdala
right
13. caudate nucleus14. hippocampus 15. medulla oblongata
16. putamen 17. substantia 18. accumbens nucleus
nigra
19. thalamus 20. pituitary gland21. spinal cord
24. atrium, left 25. atrium, right 43. spleen
49. lung 50. placenta 51. bladder
54. testis 70. Fetal brain 71. Fetal Heart
74. Fetal Spleen
Other tissues and organs that have been tested showed no expression of
~o GP-271ike protein:
heart aorta ventricle, left
ventricle, right interventricular apex of the
heart
septum
esophagus stomach duodenum
jejunum ileum ileocecum
appendix colon, ascending colon, transverse
colon, descending rectum kidney
skeletal muscle thymus peripheral blood
leukocyte
lymph node bone marrow trachea
uterus
prostate ovary
liver pancreas adrenal gland
thyroid gland salivary gland mammary gland
HL-60 leukemia Hela S3 K-562 leukemia
MOLT-4 leukemia Burkitt's lymphoma, Burkitt's lymphoma,
Raji Daudi
SW480 colore. A549 lung carcinoma Fetal Kidney
Adenoc.
Fetal Liver Fetal Thymus Fetal Lung
SU8ST1TUTE SHEET (RULE 2b)
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
- 30 -
Figure 2:
To analyse the expression of human gp271ike polypeptide in rat tissues, a
MTN rat northern blot (clontech) was performed. A band of about 4.4 kb
s was detetced in the brain only.
Examples
Example 1
Mammalian cell expression
to The receptors of the present invention are expressed in either human
embryonic kidney 293 (HEK293) cells or adherent dhfr CHO cells. To
maximize receptor expression, typically all 5' and 3' untranslated regions
(UTRs) are removed from the receptor cDNA prior to insertion into a pCDN
or pCDNA3 vector. The cells are transfected with individual receptor
is cDNAs by lipofectin and selected in the presence of 400 mg/ml 6418. After
3 weeks of selection, individual clones are picked and expanded for further
analysis. HEK293 or CHO cells transfected with the vector alone serve as
negative controls. To isolate cell lines stably expressing the individual
receptors, about 24 clones are typically selected and analyzed by Northern
~o blot analysis. Receptor mRNAs are generally detectable in about 50% of
the 6418-resistant clones analyzed.
Example 2
Ligand bank for binding and functional assays.
A bank of over 600 putative receptor ligands has been assembled for
~s screening. The bank comprises: transmitters, hormones and chemokines
known to act via a human seven transmembrane (7TM) receptor; naturally
occurring compounds which may be putative agonists for a human 7TM
receptor, non-mammalian, biologically active peptides for which a
mammalian counterpart has not yet been identified; and compounds not
~o found in nature, but which activate 7TM receptors with unknown natural
ligands. This bank is used to initially screen the receptor for known ligands,
SUBSTITUTE SHEET (RULE 26)
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
_ ;l _
sing both functional (i.e . calcium, cAMP, microphysiometer, oocyte
electrophysiology, etc, see below) as well as binding assays.
Example 3
Ligand binding assays
s Ligand binding assays provide a direct method for ascertaining receptor
pharmacology and are adaptable to a high throughput format. The purified
ligand for a receptor is radiolabeled to high specific activity (50-2000
Ci/mmol) for binding studies. A determination is then made that the
process of radiolabeling does not diminish the activity of the ligand towards
io its receptor. Assay conditions for buffers, ions, pH and other modulators
such as nucleotides are optimized to establish a workable signal to noise
ratio for both membrane and whole cell receptor sources. For these
assays, specific receptor binding is defined as total associated radioactivity
minus the radioactivity measured in the presence of an excess of unlabeled
~s competing ligand. Where possible, more than one competing ligand is
used to define residual nonspecific binding.
Example 4
Functional assay in Xenopus oocytes
Capped RNA transcripts from linearized plasmid templates encoding the
~o receptor cDNAs of the invention are synthesized in vitro with RNA
polymerases in accordance with standard procedures. In vitro transcripts
are suspended in water at a final concentration of 0.2 mg/ml. Ovarian lobes
are removed from adult female toads, Stage V defolliculated oocytes are
obtained, and RNA transcripts (10 ng/oocyte) are injected in a 50 n1 bolus
?s using a microinjection apparatus. Two electrode voltage clamps are used
to measure the currents from individual Xenopus oocytes in response to
agonist exposure. Recordings are made in Ca2+ free Barth's medium at
room temperature. The Xenopus system can be used to screen known
ligands and tissue/cell extracts for activating ligands.
3o Example 5
Microphysiometric assays
SU8ST1TUTE SHEET (RULE 26)
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
- 32 -
Activation of a wide variety of secondary messenger systems results in
extrusion of small amounts of acid from a cell. The acid formed is largely
as a result of the increased metabolic activity required to fuel the
intracellular signaling process. The pH changes in the media surrounding
s the cell are very small but are detectable by the CYTOSENSOR
microphysiometer (Molecular Devices Ltd., Menlo Park, CA). The
CYTOSENSOR is thus capable of detecting the activation of a receptor
which is coupled to an energy utilizing intracellular signaling pathway such
as the G-protein coupled receptor of the present invention.
lo Example 6
Extract/cell supernatant screening
A large number of mammalian receptors exist for which there remains, as
yet, no cognate activating ligand (agonist). Thus, active ligands for these
receptors may not be included within the ligands banks as identified to date.
is Accordingly, the 7TM receptor of the invention is also functionally
screened
(using calcium, cAMP, microphysiometer, oocyte electrophysiology, etc.,
functional screens) against tissue extracts to identify natural ligands.
Extracts that produce positive functional responses can be sequencially
subfractionated until an activating ligand is isolated identified.
2o Example 7
Calcium and cAMP functional assays
7TM receptors which are expressed in HEK 293 cells have been shown to
be coupled functionally to activation of PLC and calcium mobilization and/or
cAMP stimuation or inhibition. Basal calcium levels in the HEK 293 cells in
2s receptor-transfected or vector control cells were observed to be in the
normal, 100 nM to 200 nM, range. HEK 293 cells expressing recombinant
receptors are loaded with fura 2 and in a single day > 150 selected ligands
or tissue/cell extracts are evaluated for agonist induced calcium
mobilization. Similarly, HEK 293 cells expressing recombinant receptors
~o are evaluated for the stimulation or inhibition of cAMP production using
standard cAMP quantitation assays. Agonists presenting a calcium
transient or cAMP flucuation are tested in vector control cells to determine
if
the response is unique to the transfected cells expressing receptor.
Example 8
SU~STtTUTE SHEET (RULE 26)
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
Expression of gp271ike polypeptide mRNA in human tisssues
Analysis was performed using a labelled cDNA derived from a gp271ike
polypeptide. The cDNA was used to probe a multiple tisssue dot blot
(clontech). The following results were obtained: the receptor mRNA is
s expressed in all brain tissues tested. In addition the gp271ike polypeptide
mRNA was detected in the atria of the heart, the spleen, lung, placenta,
bladder, and testis. Of the fetal tissues tested, gp271ike polypeptide
mRNA was found in fetal brain, heart, and spleen. The result is shown in
Figure 1.
~o
Example 9
Expression of gp271ike polypeptide mRNA in rat tisssues
To analyse the expression of human gp271ike polypeptide in rat tissues, a
MTN rat northern blot (clontech) was used. A labelled cDNA probe
~s derived from gp271ike polypeptide was used to detect the corresponding
rat mRNA. A band of about 4.4 kb was detetced in the brain only. The
result is shown in Figure 2
SU8ST1TUTE SHEET (RULE 26)
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
-1-
SEQUENCE LISTING
<110> Merck Patent GmbH
<120> GP27- like ploypeptide
<130> GP271ikeFWKWS
<140>
<141>
<160> 2
<170> PatentIn Ver. 2.1
<210> 1
<211> 1113
<21L> U~IH
<213> Homo sapiens
<220>
<221> CDS
<222> (1)..(1113)
<400> 1
atg gcg aac tat agc cat gcagetgac aacattttg caaaatctc tcg
48
Met Ala Asn Tyr Ser His AlaAlaAsp AsnIleLeu GlnAsnLeu Ser
1 5 10 15
cct cta aca gcc ctg aaactgact tccttgggt ttcataata gga
ttt 96
Pro Leu Thr Ala Phe Leu LysLeuThr SerLeuGly PheIleIle Gly
20 25 30
gtc agc gtg gtg ggc aac ctc ctg atc tcc att ttg cta gtg aaa gat 144
Val Ser Val Val Gly Asn Leu Leu Ile Ser Ile Leu Leu Val Lys Asp
35 40 45
aag acc ttg cat aga gca cct tac tac ttc ctg ttg gat ctt tgc tgt 192
Lys Thr Leu His Arg Ala Pro Tyr Tyr Phe Leu Leu Asp Leu Cys Cys
50 55 60
tca gat atc ctc aga tct gca att tgt ttc cca ttt gtg ttc aac tct 240
Ser Asp Ile Leu Arg Ser Ala Ile Cys Phe Pro Phe Val Phe Asn Ser
65 70 75 80
gtc aaa aat ggc tct acc tgg act tat ggg act ctg act tgc aaa gtg 288
Val Lys Asn Gly Ser Thr Trp Thr Tyr Gly Thr Leu Thr Cys Lys Val
85 90 95
att gcc ttt ctg ggg gtt ttg tcc tgt ttc cac act get ttc atg ctc 336
Ile Ala Phe Leu Gly Val Leu Ser Cys Phe His Thr Ala Phe Met Leu
100 105 110
ttc tgc atc agt gtc acc aga tac tta get atc gcc cat cac cgc ttc 384
Phe Cys Ile Ser Val Thr Arg Tyr Leu Ala Ile Ala His His Arg Phe
115 120 125
~dt dCd :'a a Ct~' aCC ttt t~ aC t t Ct Ct t dtC t t at 432
g 'gg ~ ag g g g g g g g 9
Tyr Thr Lys Arg Leu Thr Phe Trp Thr Cys Leu Ala Val Ile Cys Met
130 135 140
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
- 2 -
gtg tgg act ctg tct gtg gcc atg gca ttt ccc ccg gtt tta gac gtg 480
Val Trp Thr Leu Ser Val Ala Met Ala Phe Pro Pro Val Leu Asp Val
145 150 155 160
ggc act tac tca ttc att agg gag gaa gat caa tgc acc ttc caa cac 528
Gly Thr Tyr Ser Phe Ile Arg Glu Glu Asp Gln Cys Thr Phe Gln His
165 170 175
cge tce ttc agg get aat gat tec tta gga ttt atg ctg ett ett get 576
Arg Ser Phe Arg Ala Asn Asp Ser Leu Gly Phe Met Leu Leu Leu Ala
180 185 190
ctc atc ctc cta gcc aca cag ctt gtc tac ctc aag ctg ata ttt ttc 624
Leu Ile Leu Leu Ala Thr Gln Leu Val Tyr Leu Lys Leu Ile Phe Phe
195 200 205
gtc cac gat cga aga aaa atg aag cca gtc cag ttt gta gca gca gtc 672
Val His Asp Arg Arg Lys Met Lys Pro Val Gln Phe Val Ala Ala Val
210 215 220
agc cag aac tgg act ttt cat ggt cct gga gcc agt ggc cag gca get 720
Ser Gln Asn Trp Thr Phe His Gly Pro Gly Ala Ser Gly Gln Ala Ala
225 230 235 240
gcc aat tgg cta gca gga ttt gga agg ggt ccc aca cca ccc acc ttg 768
Ala Asn Trp Leu Ala Gly Phe Gly Arg Gly Pro Thr Pro Pro Thr Leu
245 250 255
ctg ggc atc agg caa aat gca aac acc aca ggc aga aga agg cta ttg 816
Leu Gly Ile Arg Gln Asn Ala Asn Thr Thr Gly Arg Arg Arg Leu Leu
260 265 270
gtc tta gac gag ttc aaa atg gag aaa aga atc agc aga atg ttc tat 864
Val Leu Asp Glu Phe Lys Met Glu Lys Arg Ile Ser Arg Met Phe Tyr
275 280 285
ata atg act ttt ctg ttt cta acc ttg tgg ggc ccc tac ctg gtg gcc 912
Ile Met Thr Phe Leu Phe Leu Thr Leu Trp Gly Pro Tyr Leu Val Ala
290 295 300
tgt tat tgg aga gtt ttt gca aga ggg cct gta gta cca ggg gga ttt 960
Cys Tyr Trp Arg Val Phe Ala Arg Gly Pro Val Val Pro Gly Giy Phe
305 310 315 320
cta aca get get gtc tgg atg agt ttt gec caa gca gga atc aat cet 1008
Leu Thr Ala Ala Val Trp Met Ser Phe Ala Gln Ala Gly Ile Asn Pro
325 330 335
ttt gtc tgc att ttc tca aac agg gag ctg agg cgc tgt ttc agc aca 1056
Phe Val Cys Ile Phe Ser Asn Arg Glu Leu Arg Arg Cys Phe Ser Thr
340 345 350
acc ctt ctt tac tgc aga aaa tcc agg tta cca agg gaa cct tac tgt 11V~
Thr Leu Leu Tyr Cys Arg Lys Ser Arg Leu Pro Arg Glu Pro Tyr Cys
355 360 365
gtt ata tga 1113
Val I1 a
370
CA 02389672 2002-05-O1
WO 01/32865 PCT/EP00/10565
- 3 -
<210> 2
<211> 370
<212> PRT
<213> Homo sapiens
<400>
2
Met Ala AsnTyr SerHisAla AlaAspAsn IleLeuGln AsnLeuSer
1 5 10 15
Pro Leu ThrAla PheLeuLys LeuThrSer LeuGlyPhe IleIleGly
20 25 30
Val Ser ValVal GlyAsnLeu LeuIleSer IleLeuLeu ValLysAsp
35 40 45
Lys Thr LeuHis ArgAlaPro TyrTyrPhe LeuLeuAsp LeuCysCys
50 55 60
15Ser Asp IleLeu ArgSerAla IleCysPhe ProPheVal PheAsnSer
65 70. 75 80
Val Lys AsnGly SerThrTrp ThrTyrGly ThrLeuThr CysLysVal
85 90 9S
Ile Ala PheLeu GlyValLeu SerCysPhe HisThrAla PheMetLeu
100 105 110
Phe Cys IleSer Va1ThrArg TyrLeuAla IleAlaHis HisArgPhe
115 120 125
Tyr Thr LysArg LeuThrPhe TrpThrCys LeuAlaVal IleCysMet
130 135 140
25Val Trp ThrLeu SerValAla MetAlaPhe ProProVal LeuAspVal
145 150 155 160
Gly Thr TyrSer PheIleArg GluGluAsp GlnCysThr PheGlnHis
165 170 175
Arg Ser PheArg AlaAsnAsp SerLeuGly PheMetLeu LeuLeuAla
180 185 190
Leu Ile LeuLeu AlaThrGln LeuValTyr LeuLysLeu IlePhePhe
195 200 205
Val His AspArg ArgLysMet LysProVal GlnPheVal AlaAlaVal
210 215 220
35Ser Gln AsnTrp ThrPheHis GlyProGly AlaSerGly GlnAlaAla
225 230 235 240
Ala Asn TrpLeu AlaGlyPhe GlyArgGly ProThrPro ProThrLeu
245 250 255
Leu Gly IleArg GlnAsnAla AsnThrThr GlyArgArg ArgLeuLeu
260 265 270
Val Leu AspGlu PheLysMet GluLysArg IleSerArg MetPheTyr
275 280 285
Ile Met ThrPhe LeuPheLeu ThrLeuTrp GlyProTyr LeuValAla
290 295 300
45Cys Tyr TrpArg ValPheAla ArgGlyPro ValValPro GlyGlyPhe
305 310 315 320
Leu Thr AlaAla ValTrpMet SerPheAla GlnAlaGly IleAsnPro
325 330 335
Phe Val CysIle PheSerAsn ArgGluLeu ArgArgCys PheSerThr
340 345 350
Thr Leu LeuTyr CysArgLys SerArgLeu ProArgGlu ProTyrCys
355 360 365
Vai Ile
370
