DOCKING MECHANISM FOR NOTEBOOK-TABLET COMPUTER

MECANISME D'INSTALLATION POUR ORDINATEUR BLOC-NOTES-TABLETTE

English Abstract

A two-part docking mechanism that enables a tablet to dock onto a 'Laptop-Base
Computer'
and form a regular notebook. One part, a docking-cover, supports the tablet
when the tablet pivots,
assists the tablet to move 'in and out' of docking position and folds down to
cover the keyboard
when the tablet is not docked. The second part, an ejector plate, secures the
tablet in the tablet's
docked configuration and pushes out the tablet when the tablet is moved out of
docking position.
The docking mechanism design accommodates current laptop models and only
requires
modification to the lid of the laptop - the laptop inner components and all
peripherals require no
modification. If so desired, an optional tablet lock secures the tablet
permanently to the docking-
cover. The design also facilitates the conversion of most existing laptop
designs into 'Notebook-
Tablet' computers.

Claims

WHAT IS CLAIMED IS:
1. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
in said culture overexpresses a different gene product which is essential for
proliferation of said organism;
contesting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture.
2. The method of Claim 1, wherein said culture includes at least one strain
which does not overexpresses a gene product which is essential for
proliferation of said
organism.
3. The method of Claim 1, wherein said strains which overexpress said gene
products comprise a nucleic acid encoding said gene product which is essential
for
proliferation of said organism operably linked to a regulatable promoter.
4. The method of Claim 1, wherein said strains which overexpress said gene
products a nucleic acid encoding said gene product which is essential for
proliferation of
said organism operably linked to a constitutive promoter.
5. The method of Claim 1, wherein said identification step comprises
determining the nucleotide sequence of a nucleic acid encoding said gene
product in
said cell which proliferated more rapidly in said culture.
6. The method of Claim 1, wherein said identification step comprises
performing an amplification reaction to identify the nucleic acid encoding
said gene
product in said cell which proliferated more rapidly in said cell culture.
7. The method of Claim 6, wherein the products of said amplification reaction
are labeled with a detectable dye.
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8. The method of Claim 1, wherein said identification step comprises
performing a hybridization procedure.
9. The method of Claim 1, wherein said identification step comprises
contacting a nucleic acid array with a nucleic acid encoding said gene product
in said
cell which proliferated more rapidly in said cell culture.
10. The method of Claim 1, wherein said organism is selected from the group
consisting of bacteria, fungi, and protozoa.
11. The method of Claim 1, wherein said culture is a culture of an organism
selected from the group consisting of Anaplasma marginale, Aspergillus
fumigatus,
Bacillus arathracis, Bacterioides fragilis Bordetella pertussis, Burkholderia
cepacia,
Campylobacter jejuni, Candida albicans, Candida glabrata (also called
Torulopsis
glabrata), Candida tropicalis, Candida parapsilosis, Candida guilliermondii,
Candida
krusei, Candida kefyr (also called Candida pseudotropicalis), Candida
dubliniensis,
Chlamydia pneumoniae, Chlamydia trachomatus, Clostridium botulinum,
Clostridium
diffile, Clostridium perfringens, Coccidiodes immitis. Corynebacteriunt
diptheriae,
Cryptococcus neoformans, Enterobacter cloacae, Enterococcus faecalis,
Enterococcus
faecium, Escherichia tale, Haemophilus influenzae, Helicobacter pylori,
Histoplasma
capsulatum, Klebstella pneumoniae, Listeria monocytogenes, Mycobacterium
leprae,
Mycobacterium tuberculosis; Neisseria gonorrhoeae, Neisseria meningitides,
Nocardia
asteroides, Pasteurella haemolytica, Pasteurella multocida, Pneumocystis
carinii,
Proteus vulgaris, Pseudomonas aeruginosa, Salmonella bongori, Salmonella
cholerasuis, Salmonella enterica, Salmonella paratyphi, Salmonella typhi,
Salmonella
typhimurium, Staphylococcus aureus, Moxarella catarrhalis, Shigella boydii,
Shigella
dysenteriae, Shigella flexneri, Shigella sonnei, Staphylococcus epidermidis,
Streptococcus pneumoniae, Streptococcus mutans, Treponema pallidum, Yersinia
enterocolitica, and Yersinia pestis.
12. The method of Claim 1, wherein said compound is obtained from a library of
natural compounds.
13. The method of Claim 1, wherein said compound is obtained from a library of
synthetic compounds.
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14. The method of Claim 1, wherein said compound is present in a crude or
partially purified state.

15. The method of Claim 1, further comprising determining whether said gene
product in said strain which proliferated more rapidly in said culture has a
counterpart in
at least one other organism.

16. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
in said culture overexpresses a different gene product which is essential for
proliferation of said organism wherein said culture comprises a strain in
which a
gene product whose activity or level is inhibited by a nucleic acid comprising
a
nucleotide sequence selected from the group consisting of SEQ ID NOs.: 8-3795
is overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture.

17. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism ants comprising:
obtaining a culture comprising a plurality of strains wherein each strain
in said culture overexpresses a different gene product which is essential for
proliferation of said organism wherein said culture comprises a strain in
which a
gene product encoded by a nucleic acid comprising a nucleotide sequence
selected from the group consisting of SEQ ID NOs.; 3796-3800, 3806-4860,
5916-10012, and 14111-14944 is overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress

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said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture.

18. A method far identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
in said culture overexpresses a different gene product which is essential for
proliferation of said organism, wherein said culture comprises a strain in
which a
gene product comprising an amino acid sequence selected from the group
consisting of SEQ ID NOs.: 3801-3805, 4861-5915, 10013-14110 and 14945-
15778 is overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture.

19. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
in said culture overexpresses a different gene product which is essential for
proliferation of said organism, wherein said culture comprises a strain in
which a
gene product selected from the group consisting of a gene product having at
least 70% nucleotide sequence identity as determined using BLASTN version
2.0 with the default parameters to a gene product whose expression is
inhibited
by an antisense nucleic acid comprising a nucleotide sequence selected from
the

16747



group consisting of SEQ ID NOs.: 8-3795, a gene product encoded by a nucleic
acid having at least 70% nucleotide sequence identity as determined using
BLASTN version 2.0 with the default parameters to a nucleic acid encoding a
gene product whose expression is inhibited by an antisense nucleic acid
comprising a nucleotide sequence selected from the group consisting of SEQ ID
NOs: 8-3795, a gene product having at least 25% amino acid identity as
determined using FASTA version 3.0t78 with the default parameters to a gene
product whose expression is inhibited by an antisense nucleic acid comprising
a
nucleotide sequence selected from the group consisting of SEQ ID NOs.: 8-
3795, a gene product encoded by a nucleic acid which hybridizes to a nucleic
acid comprising a nucleotide sequence selected from the group consisting of
SEQ ID NOs.: 8-3795 under stringent conditions, a gene product encoded by a
nucleic acid which hybridizes to a nucleic acid comprising a nucleotide
sequence
selected from the group consisting of SEQ ID NOs.: 8-3795 under moderate
conditions, and a gene product whose activity may be complemented by the
gene product whose activity is inhibited by a nucleic acid comprising a
nucleotide sequence selected from the group consisting of SEQ ID NOs: 8-3795
is overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product an which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture.

20. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
in said culture overexpresses a different gene product which is essential for
proliferation of said organism, wherein paid culture comprises a strain in
which a

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gene product encoded by a nucleic acid comprising a nucleotide sequence
selected from the group consisting of a nucleic acid comprising a nucleic acid
having at least 70% nucleotide sequence identity as determined using BLASTN
version 2.0 with the default parameters to a nucleotide sequence selected from
the group consisting of SEQ ID NOS.: 3796-3800, 3806-4860, 5916-10012, and
14111-14944, a nucleic acid comprising a nucleotide sequence which hybridizes
to a sequence selected from the group consisting of SEQ ID NOS.: 3796-3800,
3806-4860, 5916-10012, and 14111-14944 under stringent conditions, and a
nucleic acid comprising a nucleotide sequence which hybridizes to a nucleotide
sequence selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-
4860, 5916-10012, and 14111-14944 under moderate conditions is
overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture.

21. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
in said culture overexpresses a different gene product which is essential for
proliferation of said organism, wherein said culture comprises a strain in
which a
gene product comprises a polypeptide selected from the group consisting of a
polypeptide having at least 25% amino acid identity as determined using
FASTA version 3.0t78 to a polypeptide selected from the group consisting of
SEQ ID NOs.: 3801-3805, 4861-5915, 10013-14110 and 14945-15778 and a
polypeptide whose activity may be complemented by a polypeptide selected

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from the group consisting of SEQ ID NOs: 3801-3805, 4861-5915, 10013-
14110 and 14945-15778 is overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture.

22. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining an array of strains on a solid growth medium wherein each
strain in overexpresses a different gene product which is essential for
proliferation of said organism
contacting said array of strains with a sufficient concentration of said
compound to inhibit the proliferation of strains of said organism which do not
overexpress said gene product on which said compound acts, such that strains
which overexpress said gene product on which said compound acts proliferate
more rapidly than strains which do not overexpress said gene product on which
said compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly on said solid medium.

23. The method of Claim 21, wherein at least one strain in said array does not
overexpresses a gene product which is essential for proliferation of said
organism.

24. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a plurality of cultures, wherein each culture comprises a
plurality of strains wherein each strain overexpresses a different gene
product
which is essential for proliferation of said organism;

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contacting each of said cultures with a different concentration of said
compound ; and
identifying the gene product which is overexpressed in a strain whose
proliferation is inhibited by said compound.

25. The method of Claim 23, wherein at least one strain in said plurality of
cultures does not overexpress a gene product which is essential for
proliferation of said
organism.

26. A method of profiling a compound's activity comprising
performing the method of Claim 1 on a first culture using a first
compound;
performing the method of Claim 1 on a second culture using a second
compound; and
comparing the strains identified in said first culture to the strains
identified in said second culture.

27. A method of profiling a first compound's activity comprising
growing an array of strains on a first solid medium comprising said first
compound and on a second solid medium comprising a second compound,
wherein each strain in said array overexpresses a different gene product which
is
essential for proliferation of an organism and wherein said first compound and
said second compound inhibit the proliferation of said organism; and
comparing the pattern of strains which grow on said first solid medium
with the pattern of strains which grow on said second solid medium.

28. The method of any one of Claims 26 and 27, wherein said first compound is
present in a crude or partially purified state.

29. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said

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gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress said gene product on which said compound
acts; and
identifying the gene product which is underexpressed in a strain which
proliferated more slowly in said culture.

30. The method of Claim 29, wherein at least one strain in said culture does
not
underexpresses a gene product which is essential for proliferation of said
organism.

31. The method of Claim 29, wherein said strains which underexpreses said
gene products comprise a nucleic acid complementary to at least a portion of a
gene
encoding said gene product which is essential for proliferation of said
organism
operably linked to a regulatable promoter.

32. The method of Claim 29, wherein said strains which underexpress said gene
products express an antisense nucleic acid complementary to at least a portion
of a gene
encoding said gene product which is essential for proliferation of said
organism,
wherein expression of said antisense nucleic acid reduces expression of said
gene
product in said strain.

33. The method of Claim 29, wherein said identification step comprises
determining the nucleotide sequence of a nucleic acid encoding said gene
product in
said strain which proliferated more slowly.

34. The method of Claim 29, wherein said identification step comprises
performing an amplification reaction to identify the nucleic acid encoding
said gene
product in said cell which proliferated more slowly.

35. The method of Claim 34, wherein the products of said amplification
reaction
are labeled with a detectable dye.

36. The method of Claim 29, wherein said identification step comprises
performing a hybridization procedure.

37. The method of Claim 29, wherein said identification step comprises
contacting a nucleic acid array with a nucleic acid encoding said gene product
in said
cell which proliferated more slowly.

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38. The method of Claim 29, wherein said organism is selected from the group
consisting of bacteria, fungi, protozoa.

39. The method of Claim 29, wherein said compound is obtained from a library
of natural compounds.

40. The method of Claim 29, wherein said compound is obtained from a library
of synthetic compounds.

41. The method of Claim 29, wherein said compound is present in a crude or
partially purified state.

42. The method of Claim 29, further comprising determining whether said gene
product in said strain which proliferated more slowly in said culture has a
counterpart in
at least one other organism.

43. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism wherein said culture comprises a strain in which a gene product
whose activity or level is inhibited by a nucleic acid comprising a nucleotide
sequence selected from the group consisting of SEQ ID NOs.: 8-3795 is
underexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress said gene product on which said compound
acts; and
identifying the gene product which is underexpressed in a strain which
proliferated more slowly in said culture.

44. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of

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said organism wherein said culture comprises a strain in which a gene product
encoded by a nucleic acid comprising a nucleotide sequence selected from the
group consisting of SEQ ID NOs.: 3796-3800, 3806-4860, 5916-10012, and
14111-14944 is underexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress said gene product on which said compound
acts; and
identifying the gene product which is underexpressed in a strain which
proliferated more slowly in said culture.

45. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism wherein said culture comprises a strain in which a gene product
comprising an amino acid sequence selected from the group consisting of SEQ
ID NOs.: 3801-3805, 4861-5915, 10013-14110 and 14945-15778 is
underexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress said gene product on which said compound
acts; and
identifying the gene product which is underexpressed in a strain which
proliferated more slowly in said culture.

46. A method far identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:

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obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism wherein said culture comprises a strain in which a gene product
selected from the group consisting of a gene product having at least 70%
nucleotide sequence identity as determined using BLASTN version 2.0 with the
default parameters to a gene product whose expression is inhibited by an
antisense nucleic acid comprising a nucleotide sequence selected from the
group
consisting of SEQ ID NOs.: 8-3795, a gene product encoded by a nucleic acid
having at least 70% nucleotide sequence identity as determined using BLASTN
version 2.0 with the default parameters to a nucleic acid encoding a gene
product
whose expression is inhibited by an antisense nucleic acid comprising a
nucleotide sequence selected from the group consisting of SEQ ID NOs: 8-3795,
a gene product having at least 25% amino acid identity as determined using
FASTA version 3.0t78 with the default parameters to a gene product whose
expression is inhibited by an antisense nucleic acid comprising a nucleotide
sequence selected from the group consisting of SEQ ID NOs.: 8-3795, a gene
product encoded by a nucleic acid which hybridizes to a nucleic acid
comprising
a nucleotide sequence selected from the group consisting of SEQ ID NOs.: 8-
3795 under stringent conditions, a gene product encoded by a nucleic acid
which
hybridizes to a nucleic acid comprising a nucleotide sequence selected from
the
group consisting of SEQ ID NOs.: 8-3795 under moderate conditions, and a
gene product whose activity may be complemented by the gene product whose
activity is inhibited by a nucleic acid comprising a nucleotide sequence
selected
from the group consisting of SEQ ID NOs: 8-3795 is underexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product an which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress said gene product on which said compound
acts; and

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identifying the gene product which is underexpressed in a strain which
proliferated more slowly in said culture.

47. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential fox proliferation
of
said organism wherein said culture comprises a strain in which a gene product
encoded by a nucleic acid comprising a nucleotide sequence selected from the
group consisting of a nucleic acid comprising a nucleic acid having at least
70%
nucleotide sequence identity as determined using BLASTN version 2,0 with the
default parameters to a nucleotide sequence selected from the group consisting
of SEQ ID NOS.: 3796-3800, 3806-4860, 5916-10012, and 14111-14944, a
nucleic acid comprising a nucleotide sequence which hybridizes to a sequence
selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-4860,
5915-10012, and 14111-14944 under stringent conditions, and a nucleic acid
comprising a nucleotide sequence which hybridizes to a nucleotide sequence
selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-4860,
5916-10012, and 14111-14944 under moderate conditions is underexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress said gene product on which said compound
acts; and
identifying the gene product which is underexpressed in a strain which
proliferated more slowly in said culture.

48. A method for identifying the gene product an which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism wherein said culture comprises a strain in which a gene product


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comprises a polypeptide selected from the group consisting of a polypeptide
having at least 25% amino acid identity as determined using FASTA version
3.0t78 to a polypeptide selected from the group consisting of SEQ ID NOs.:
3801-3805, 4861-5915, 10013-14110 and 14945-15778 and a polypeptide
whose activity may be complemented by a polypeptide selected from the group
consisting of SEQ ID NOs: 3801-3805, 4861-5915, 10013-14110 and 14945-
15778 is undexexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress said gene product on which said compound
acts; and
identifying the gene product which is underexpressed in a strain which
proliferated more slowly in said culture.

49. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising;
obtaining a plurality of cultures, each culture comprising a plurality of
strains wherein each strain underexpresses a different gene product which is
essential for proliferation of said organism; and
contacting each of said cultures with a different concentration of said
compound; and
identifying the gene product which is underexpressed in a strain whose
rate of proliferation is reduced by said compound.

50. A method of profiling a compound's activity comprising
performing the method of Claim 29 an a first culture using a first
compound;
performing the method of Claim 29 on a second culture using a second
compound; and
comparing the strains identified in said first culture to the strains
identified in said second culture.


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51. A method of profiling a first compound's activity comprising
growing an array of strains on a first solid medium comprising said first
compound and on a second solid medium comprising a second compound,
wherein said array comprises a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of an
organism and wherein said first compound and said second compound inhibit
the proliferation of said organism; and
comparing the pattern of strains which grow on said first solid medium
with the pattern of strains which grow on said second solid medium.

52. The method of any one of Claims 49 and 50, wherein said first compound is
present in a crude or partially purified state.

53. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising;
obtaining a plurality of cultures comprising a plurality of strains wherein
each strain underexpresses a different gene product which is essential for
proliferation of said organism;
contacting each of said plurality of cultures with a varying concentration
of a regulatory agent which regulates the level of expression of said gene
products which are essential for proliferation of said organism ; and
identifying the gene product which is underexpressed in a strain whose
rate of proliferation is reduced by said compound.

54. A culture comprising a plurality of strains wherein each strain
overexpresses
a different gene product which is essential for proliferation of said
organism.
55. The culture of Claim 54, wherein said strains which overexpresess said
gene
products comprise a nucleic acid encoding said gene product which is essential
for
proliferation of said organism operably linked to a regulatable promoter.

55. The culture of Claim 54, wherein said strains which overexpresess said
gene
products comprise a nucleic acid encoding said gene product which is essential
for
proliferation of said organism operably linked to a constitutive promoter.

57. The culture of Claim 54, wherein said culture is a culture of an organism
selected from the group consisting of Anaplasma marginale, Aspergillus
fumigatus,



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Bacillus anthracis, Bacterioides fragilis Bordetella pertussis, Burkholderia
cepacia,
Campylobacter jejuni, Candida albicans, Candida glabrata (also called
Torulopsis
glabrata), Candida tropicalis, Candida parapsilosis, Candida guilliermondii,
Candida
krusei, Candida kefyr (also called Candida pseudotropicalis), Candida
dubliniensis,
Chlamydia pneumoniae, Chlamydia trachomatus, Clostridium botulinum,
Clostridium
difficile, Clostridium perfringens, Coccidiodes immitis, Corynebacterium
diptheriae,
Cryptococcus neoformans, Enterobacter cloacae, Enterococcus faecalis,
Enterococcus
faecium, Escherichia coli, Haemophilus influenzae, Helicobacter pylori,
Histoplasma
capsulatum, Klebsiella pneumoniae, Listeria monocytogenes, Mycobacterium
leprae,
Mycobacterium tuberculosis, Neisseria gonorrhoeae, Neisseria meningitidis,
Nocardia
asteroides, Pasteurella haemolytica, Pasteurella multocida, Pneumocystis
carinii,
Proteus vulgaris, Pseudomonas aeruginosa, Salmonella bongori, Salmonella
cholerasuis, Salmonella entertca, Salmonella paratyphi, Salmonella typhi,
Salmonella
typhimurium, Staphylococcus aureus, Maxarella catarrhalis, Shigella boydii,
Shigella
dysenteriae, Shigella flexneri, Shigella sonnei, Staphylococcus epidermidis,
Streptococcus pneumoniae, Streptococcus mutans, Treponema pallidum, Yersinia
enterocolitica, and Yersinia pestis.

58. A culture comprising a plurality of strains wherein each strain
overexpresses
a different gene product which is essential for proliferation of said
organism, wherein
said culture comprises a strain in which a gene product whose activity or
level is
inhibited by a nucleic acid comprising a nucleotide sequence selected from the
group
consisting of SEQ ID NOs.: 8-3795 is overexpressed.

59. A culture comprising a plurality of strains wherein each strain
overexpresses
a different gene product which is essential for proliferation of said
organism, wherein
said culture comprises a strain in which a gene product encoded by a nucleic
acid
comprising a nucleotide sequence selected from the group consisting of SEQ ID
NOs.:
3796-3800, 380b-4860, 5916-10012, and 14111-14944 is overexpressed.

60. A culture comprising a plurality of strains wherein each strain
overexpresses
a different gene product which is essential for proliferation of said
organism, wherein
said culture comprises a strain in which a gene product comprising an amino
acid


16759




sequence selected from the group consisting of SEQ ID NOs.: 3801-3805, 4861-
5915,
10013-14110 and 14945-15778 is overexpressed.

61. A culture comprising a plurality of strains wherein each strain
overexpresses
a different gene product which is essential for proliferation of said
organism, wherein
said culture comprises a strain in which a gene product selected from the
group
consisting of a gene product having at least 70% nucleotide sequence identity
as
determined using BLASTN version 2.0 with the default parameters to a gene
product
whose expression is inhibited by an antisense nucleic acid comprising a
nucleotide
sequence selected from the group consisting of SEQ ID NOs.: 8-3795, a gene
product
encoded by a nucleic acid having at least 70% nucleotide sequence identity as
determined using BLASTN version 2.0 with the default parameters to a nucleic
acid
encoding a gene product whose expression is inhibited by an antisense nucleic
acid
comprising a nucleotide sequence selected from the group consisting of SEQ ID
NOs:
8-3795, a gene product having of least 25% amino acid identity as determined
using
FASTA version 3.0t78 with the default parameters to a gene product whose
expression
is inhibited by an antisense nucleic acid comprising a nucleotide sequence
selected from
the group consisting of SEQ ID NOs.: 8-3795, a gene product encoded by a
nucleic acid
which hybridizes to a nucleic acid comprising a nucleotide sequence selected
from the
group consisting of SEQ ID NOs.: 8-3795 under stringent conditions, a gene
product
encoded by a nucleic acid which hybridizes to a nucleic acid comprising a
nucleotide
sequence selected from the group consisting of SEQ ID NOs.: 8-3795 under
moderate
conditions, and a gene product whose activity may be complemented by the gene
product whose activity is inhibited by a nucleic acid comprising a nucleotide
sequence
selected from the group consisting of SEQ ID NOs: 8-3795 is overexpressed.

62. A culture comprising a plurality of strains wherein each strain
overexpresses
a different gene product which is essential for proliferation of said
organism, wherein
said culture comprises a strain in which a gene product encoded by a nucleic
acid
comprising a nucleotide sequence selected from the group consisting of a
nucleic acid
comprising a nucleic acid having at least 70% nucleotide sequence identity as
determined using BLASTN version 2.0 with the default parameters to a
nucleotide
sequence selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-
4860,



16760




5916-10012, and 14111-14944, a nucleic acid comprising a nucleotide sequence
which
hybridizes to a sequence selected from the group consisting of SEQ ID NOS.:
3796-
3800, 3806-4860, 5916-10012, and 14111-14944 under stringent conditions, and a
nucleic acid comprising a nucleotide sequence which hybridizes to a nucleotide
sequence selected from the group consisting of SEQ ID NOS,: 3796-3800, 3806-
4860,
5916-10012, and 14111-14944 under moderate conditions is overexpressed.

63. A culture comprising a plurality of strains wherein each strain
overexpresses
a different gene product which is essential for proliferation of said
organism, wherein
said culture comprises a strain in which a gene product comprises a
polypeptide selected
from the group consisting of a polypeptide having at least 25% amino acid
identity as
determined using FASTA version 3.0t78 to a polypeptide selected from the group
consisting of SEQ ID NOs.: 3801-3805, 4861-5915, 10013-14110 and 14945-15778
and
a polypeptide whose activity may be complemented by a polypeptide selected
from the
group consisting of SEQ ID NOs: 3801-3805, 4861-5915, 10013-14110 and 14945-
15778 is overexpressed.

64. A culture comprising a a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of said
organism.

65. The culture of Claim 64, wherein said strains which underexpress said gene
products comprise a nucleic acid encoding said gene product which is essential
for
proliferation of said organism operably linked to a regulatable promoter.

66. The culture of Claim 64, wherein said strains which underexpress said gene
products comprise a nucleic acid encoding said gene product which is essential
for
proliferation of said organism operably linked to a constitutive promoter.

67. The culture of Claim 64, wherein said culture is a culture of an organism
selected from the group consisting of Anaplasma marginale, Aspergillus
fumigatus,
Bacillus anthracis, Bacterioides fragilis Bordetella pertussis, Burkholderia
cepacia,
Campylobacter jejuni, Candida albicans, Candida glabrata (also called
Torulopsis
glabrata), Candida tropicalis, Candida parapsilosis, Candida guilliermondii,
Candida
krusei, Candida kefyr (also called Candida pseudotropicalis), Candida
dubliniensis,
Chlamydia pneumoniae, Chlamydia trachomatus, Clostridium botulinum,
Clostridium


16761




difficile, Clostridium perfringens, Coccidiodes immitis, Corynebacterium
diptheriae,
Cryptococcus neoformans, Enterobacter cloacae, Enterococcus faecalis,
Enterococcus
faecium, Escherichia coli, Haemophilus influenzae, Helicobacter pylori,
Histoplasma
capsulatum, Klebsiella pneumoniae, Listeria monocytogenes, Mycobacterium
leprae,
Mycobacterium tuberculosis, Neisseria gonorrhoeae, Neisseria meningitidis,
Nocardia
asteroides, Pasteurella haemolytica, Pasteurella multocida, Pneumocystis
carinii,
Proteus vulgaris, Pseudomonas aeruginosa, Salmonella bongori, Salmonella
cholerasuis, Salmonella enterica, Salmonella paratyphi, Salmonella typhi,
Salmonella
typhimurium, Staphylococcus aureus, Moxarella catarrhalis, Shigella boydii,
Shigella
dysenteriae, Shigella flexneri, Shigella sonnei, Staphylococcus epidermidis,
Streptococcus pneumoniae, Streptococcus mutans, Treponema pallidum, Yersinia
enterocolitica, and Yersinia pestis.

68. A culture comprising a a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of said
organism, wherein said culture comprises a strain in which a gene product
whose
activity or level is inhibited by a nucleic acid comprising a nucleotide
sequence selected
from the group consisting of SEQ ID NOs.: 8-3795 is underexpressed.

69. A culture comprising a a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of said
organism, wherein said culture comprises a strain in which a gene product
encoded by a
nucleic acid comprising a nucleotide sequence selected from the group
consisting of
SEQ ID NOs.: 3796-3800, 3806-4860, 5916-10012, and 14111-14944 is
underexpressed,

70. A culture comprising a a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of said
organism, wherein said culture comprises a strain in which a gene product
comprising
an amino acid sequence selected from the group consisting of SEQ 177 NOs.:
3801-
3805, 4861-5915, 10013-14110 and 14945-15778 is underexpressed.

71. A culture comprising a a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of said
organism, wherein said culture comprises a strain in which a gene product
selected from


16762



the group consisting of a gene product having at least 70% nucleotide sequence
identity
as determined using BLASTN version 2.0 with the default parameters to a gene
product
whose expression is inhibited by an antisense nucleic acid comprising a
nucleotide
sequence selected from the group consisting of SEQ ID NOs.: 8-3795, a gene
product
encoded by a nucleic acid having at Least 70% nucleotide sequence identity as
determined using BLASTN version 2.0 with the default parameters to a nucleic
acid
encoding a gene product whose expression is inhibited by an antisense nucleic
acid
comprising a nucleotide sequence selected from the group consisting of SEQ ID
NOs:
8-3795, a gene product having at least 25% amino acid identity as determined
using
FASTA version 3.0t78 with the default parameters to a gene product whose
expression
is inhibited by an antisense nucleic acid comprising a nucleotide sequence
selected from
the group consisting of SEQ ID NOs.: 8-3795, a gene product encoded by a
nucleic acid
which hybridizes to a nucleic acid comprising a nucleotide sequence selected
from the
group consisting of SEQ ID NOs.: 8-3795 under stringent conditions, a gene
product
encoded by a nucleic acid which hybridizes to a nucleic acid comprising a
nucleotide
sequence selected from the group consisting of SEQ ID NOs.: 8-3795 under
moderate
conditions, and a gene product whose activity may be complemented by the gene
product whose activity is inhibited by a nucleic acid comprising a nucleotide
sequence
selected from the group consisting of SEQ ID NOs: 8-3795 is underexpressed.

72. A culture comprising a a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of said
organism, wherein said culture comprises a strain in which a gene product
encoded by a
nucleic acid comprising a nucleotide sequence selected from the group
consisting of a
nucleic acid comprising a nucleic acid having at least 70% nucleotide sequence
identity
as determined using BLASTN version 2.0 with the default parameters to a
nucleotide
sequence selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-
4860,
5916-10012, and 14111-14944, a nucleic acid comprising a nucleotide sequence
which
hybridizes to a sequence selected from the group consisting of SEQ ID NOS.:
3796-
3800, 3806-4860, 5916-20012, and 14111-14944 under stringent conditions, and a
nucleic acid comprising a nucleotide sequence which hybridizes to a nucleotide


16763




sequence selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-
4860,
5916-10012, and 14111-14944 under moderate conditions is underexpressed.

73. A culture comprising a a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of said
organism, wherein said culture comprises a strain in which a gene product
comprises a
polypeptide selected from the group consisting of a polypeptide having at
least 25%
amino acid identity as determined using FASTA version 3.0t78 to a polypeptide
selected from the group consisting of SEQ ID NOs.: 3801-3805, 4861-5915, 10013-

14110 and 14945-15778 and a polypeptide whose activity may be complemented by
a
polypeptide selected from the group consisting of SEQ ID NOs: 3801-3805, 4861-
5915,
10013-14110 and 14945-15778 is underexpressed.

74. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
overexpresses a different gene product which is essential for proliferation of
said
organism and wherein the nucleotide sequence of each of the overexpressed
genes has been altered so as to include a nucleotide sequence which can be
used
to generate a unique product corresponding to each of the overexpressed genes;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

75. The method of Claim 74, wherein the nucleotide sequence of each of the
genes encoding an overexpressed gene product has been altered by replacing the
native
promoters of said genes with promoters which facilitate overexpression of said
gene
products.


16764



76. The method of Claim 74, wherein the nucleotide sequence of each of the
genes encoding an overexpressed gene product has been altered by inserting a
regulatory element into the native promoters of said genes with a promoter
which
facilitates overexpression of said gene products.

77. The method of Claim 76, wherein said regulatory element is selected from
the group consisting of a regulatable promoter, an operator which is
recognized by a
repressor, a nucleotide sequence which is recognized by a transcriptional
activator, a
transcriptional terminator, a nucleotide sequence which introduces a bend in
the DNA
and an upstream activating sequence.

78. The method of Claim 74, wherein the step of identifying the gene product
which is overexpressed in a strain which proliferated more rapidly in said
culture by
detecting the unique product corresponding to said gene comprises performing
an
amplification reaction and detecting a unique amplification product
corresponding to
said gene.

79. The method of Claim 75, wherein the native promoter of each of the genes
encoding a gene product essential for proliferation is replaced with the same
promoter.

80. The method of Claim 75, wherein the native promoters of the genes
encoding gene products essential for proliferation are replaced with a
plurality of
promoters selected to give a desired expression level for each gene product.

81. The method of Claim 75, wherein said promoters which replaced the native
promoters in each strain comprise regulatable promoters.

82. The method of Claim 75, wherein said promoters which replaced the native
promoters in each strain each strain comprise constitutive promoters.

83. The method of Claim 74, wherein said organism is selected from the group
consisting of bacteria, fungi, and protozoa.

84. The method of Claim 74, wherein said culture is a culture of an organism
selected from the group consisting of Anaplasma marginale, Aspergillus
fumigatus,
Bacillus anthracis, Bacterioides fragilis Bordetella pertussis, Burkholderia
cepacia,
Campylobacter jejuni, Candida albicans, Candida glabrata (also called
Torulopsis
glabrata), Candida tropicalis, Candida parapsilosis, Candida guilliermondii,
Candida
krusei, Candida kefyr (also called Candida pseudotropicalis), Candida
dubliniensis,


16765


Chlamydia pneumoniae, Chlamydia trachomatus, Clostridium botulinum,
Clostridium
difficile, Clostridium perfringens, Coccidiodes immitis, Corynebacterium
diptheriae,
Cryptococcus neoformans, Enterobacter cloacae, Enterococcus faecalis,
Enterococcus
faecium, Escherichia coli, Haemophilus influenzae, Helicobacter pylori,
Histoplasma
capsulatum, Klebsiella pneumoniae, Listeria monocytogenes, Mycobacterium
leprae,
Mycobacterium tuberculosis, Neisseria gonorrhoeae, Neisseria meningitidis,
Nocardia
asteroides, Pasteurella haemolytica, Pasteurella multocida, Pneumocystis
carinii,
Proteus vulgaris, Pseudomonas aeruginosa, Salmonella bongori, Salmonella
cholerasuis, Salmonella enterica, Salmonella paratyphi, Salmonella typhi,
Salmonella
typhimurium, Staphylococcus aureus, Moxarella catarrhalis, Shigella boydii,
Shigella
dysenteriae, Shigella flexneri, Shigella sonnei, Staphylococcus epidermidis.
Streptococcus pneumoniae, Streptococcus mutans, Treponema pallidum, Yersinia
enterocolitica, and Yersinia pestis.

85. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
overexpresses a different gene product which is essential for proliferation of
said
organism and wherein the nucleotide sequence of each of the overexpressed
genes has been altered so as to include a nucleotide sequence which can be
used
to generate a unique product corresponding to each of the overexpressed genes,
wherein said culture comprises a strain in which a gene product whose activity
or level is inhibited by a nucleic acid comprising a nucleotide sequence
selected
from the group consisting of SEQ ID NOs.: 8-3795 is overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and

16766


identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

86. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
overexpresses a different gene product which is essential for proliferation of
said
organism and wherein the nucleotide sequence of each of the overexpressed
genes has been altered so as to include a nucleotide sequence which can be
used
to generate a unique product corresponding to each of the overexpressed genes,
wherein said culture comprises a strain in which a gene product encoded by a
nucleic acid comprising a nucleotide sequence selected from the group
consisting of SEQ ID NOs.: 3796-3800, 3806-4860, 5916-10012, and 14111-
14944 is overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

87. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
overexpresses a different gene product which is essential for proliferation of
said
organism and wherein the nucleotide sequence of each of the overexpressed
genes has been altered so as to include a nucleotide sequence which can be
used
to generate a unique product corresponding to each of the overexpressed genes,
wherein said culture comprises a strain in which a gene product comprising an

16767


amino acid sequence selected from the group consisting of SEQ ID NOs.: 3801-
3805, 4861-5915, 10013-141 10 and 14945-15778 is overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

88. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
overexpresses a different gene product which is essential for proliferation of
said
organism and wherein the nucleotide sequence of each of the overexpressed
genes has been altered so as to include a nucleotide sequence which can be
used
to generate a unique product corresponding to each of the overexpressed genes,
wherein said culture comprises a strain in which a gene product selected from
the group consisting of a gene product having at least 70% nucleotide sequence
identity as determined using BLASTN version 2.0 with the default parameters to
a gene product whose expression is inhibited by an antisense nucleic acid
comprising a nucleotide sequence selected from the group consisting of SEQ ID
NOs.: 8-3795, a gene product encoded by a nucleic acid having at least 70%
nucleotide sequence identity as determined using BLASTN version 2,0 with the
default parameters to a nucleic acid encoding a gene product whose expression
is inhibited by an antisense nucleic acid comprising a nucleotide sequence
selected from the group consisting of SEQ ID NOs: 8-3795, a gene product
having at least 25% amino acid identity as determined using FASTA version
3.0t78 with the default parameters to a gene product whose expression is
inhibited by an antisense nucleic acid comprising a nucleotide sequence
selected

16768


from the group consisting of SEQ ID NOs.: 8-3795, a gene product encoded by a
nucleic acid which hybridizes to a nucleic acid comprising a nucleotide
sequence
selected from the group consisting of SEQ ID NOs.: 8-3795 under stringent
conditions, a gene product encoded by a nucleic acid which hybridizes to a
nucleic acid comprising a nucleotide sequence selected from the group
consisting of SEQ ID NOs.: 8-3795 under moderate conditions, and a gene
product whose activity may be complemented by the gene product whose
activity is inhibited by a nucleic acid comprising a nucleotide sequence
selected
from the group consisting of SEQ ID NOs: 8-3795 is overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

89. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
overexpresses a different gene product which is essential for proliferation of
said
organism and wherein the nucleotide sequence of each of the overexpressed
genes has been altered so as to include a nucleotide sequence which can be
used
to generate a unique product corresponding to each of the overexpressed genes;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and

16769


identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

90. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
overexpresses a different gene product which is essential for proliferation of
said
organism and wherein the nucleotide sequence of each of the overexpressed
genes has been altered so as to include a nucleotide sequence which can be
used
to generate a unique product corresponding to each of the overexpressed genes,
wherein said culture comprises a strain in which a gene product encoded by a
nucleic acid comprising a nucleotide sequence selected from the group
consisting of a nucleic acid comprising a nucleic acid having at least 70%
nucleotide sequence identity as determined using BLASTN version 2.0 with the
default parameters to a nucleotide sequence selected from the group consisting
of SEQ ID NOS.: 3796-3800, 3806-4860, 591b-10012, and 14111-14944, a
nucleic acid comprising a nucleotide sequence which hybridizes to a sequence
selected from the group consisting of SEQ ID NOS.: 3795-3800, 3806-4860,
5916-10012, and 14111-14944 under stringent conditions, and a nucleic acid
comprising a nucleotide sequence which hybridizes to a nucleotide sequence
selected from the group consisting of SEQ ID NOS.: 3796-3800, 380b-4860,
5916-10012, and 14111-14944 under moderate conditions is overexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which do not
overexpress
said gene product on which said compound acts, such that strains which
overexpress said gene product on which said compound acts proliferate more
rapidly than strains which do not overexpress said gene product on which said
compound acts; and
identifying the gene product which is overexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene, wherein said culture comprises a strain in which a

16770


gene product comprises a polypeptide selected from the group consisting of a
polypeptide having at least 25% amino acid identity as determined using
FABTA version 3.0t78 to a polypeptide selected from the group consisting of
SEQ ID NOs.; 3801-3805, 4861-S91S, 10013-14110 and 14945-15778 and a
polypeptide whose activity may be complemented by a polypeptide selected
from the group consisting of SEQ ID NOs: 3801-3805, 4861-5915, 10013-
14110 and 14945-15778 is overexpressed.

91. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism and wherein the nucleotide sequence of each of the
underexpressed genes has been altered so as to include a nucleotide sequence
which can be used to generate a unique product corresponding to each of the
overexpressed genes;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product an which said compound acts proliferate more slowly than
strains which do not underexpress the gene product on which said compound
acts; and
identifying the gene product which is underexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

92. The method of Claim 91, wherein the nucleotide sequence of each of the
genes encoding an underexpressed gene product has been altered by replacing
the native
promoters of said genes with promoters which facilitate underexpression of
said gene
products.

93. The method of Claim 91, wherein the nucleotide sequence of each of the
genes encoding an underexpressed gene product has been altered by inserting a

16771



regulatory element into the native promoters of said genes with a promoter
which
facilitates underexpression of said gene products.

94. The method of Claim 93, wherein said regulatory element is selected from
the group consisting of a regulatable promoter, an operator which is
recognized by a
repressor, a nucleotide sequence which is recognized by a transcriptional
activator, a
transcriptional terminator, a nucleotide sequence which introduces a bend in
the DNA
and an upstream activating sequence.

95. The method of Claim 91, wherein the step of identifying the gene product
which is underexpressed in a strain which proliferated more slowly in said
culture by
detecting the unique product corresponding to said gene comprises performing
an
amplification reaction and detecting a unique amplification product
corresponding to
said gene.

96. The method of Claim 92, wherein the native promoter of each of the genes
encoding a gene product essential for proliferation is replaced with the same
promoter.

97. The method of Claim 92, wherein the native promoters of the genes
encoding gene products essential for proliferation are replaced with a
plurality of
promoters selected to give a desired expression level for each gene product.

98. The method of Claim 92, wherein said promoters which replaced the native
promoters in each strain comprise regulatable promoters.

99. The method of Claim 92, wherein said promoters which replaced the native
promoters in each strain each strain comprise constitutive promoters.

100. The method of Claim 91, wherein said organism is selected from the
group consisting of bacteria, fungi, and protozoa.

101. The method of Claim 91, wherein said culture is a culture of an organism
selected from the group consisting of Anaplasma marginale, Aspergillus
fumigatus,
Bacillus anthracis, Bacterioides fragilis Bordetella pertussis, Burkholderia
cepacia,
Campylobacter jejuni, Candida albicans, Candida glabrata (also called
Torulopsis
glabrata), Candida tropicalis, Candida parapsilosis, Candida guilliermondii,
Candida
krusei, Candida kefyr (also called Candida pseudotropicalis), Candida
dubliniensis,
Chlamydia pneumoniae, Chlamydia trachomatus, Clostridium botulinum,
Clostridium
difficile, Clostridium perfringens, Coccidiodes immitis, Corynebacterium
diptheriae,

16772



Cryptococcus neoformans, Enterobacter cloacae, Enterococcus faecalis,
Enterococcus
faecium, Escherichia coli, Haemophilus influenzae, Helicobacter pylon,
Histoplasma
capsulatum, Klebsiella pneumoniae, Lisieria monocytogenes, Mycobacterium
leprae,
Mycobacterium tuberculosis, Neisseria gonorrhoeae, Neisseria meningitidis,
Nocardia
asteroides, Pasteurella haemolytica, Pasteurella multocida, Pneumocystis
curinii,
Proteus vulgaris, Pseudomonas aeruginosa, Salmonella bongori, Salmonella
cholerasuis, Salmonella enterica, Salmonella paratyphi, Salmonella typhi,
Salmonella
typhimurium, Staphylococcus aureus, Moxarella catarrhalis, Shigella boydii,
Shigella
dysenteriae, Shigella flexneri, Shigella sonnei, Staphylococcus epidermidis,
Streptococcus pneumoniae, Streptococcus mutans, Treponema pallidum, Yersinia
enterocolitica, and Yersinia pestis.

102. The method of Claim 91, wherein said culture comprises a strain in
which a gene product whose activity or level is inhibited by a nucleic acid
comprising a
nucleotide sequence selected from the group consisting of SEQ ID NOs.: 8-3795
is
underexpressed.

103. A method for identifying the gene product can which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism and wherein the nucleotide sequence of each of the
underexpressed genes has been altered so as to include a nucleotide sequence
which can be used to generate a unique product corresponding to each of the
overexpressed genes and wherein said culture comprises a strain in which a
gene
product encoded by a nucleic acid comprising a nucleotide sequence selected
from the group consisting of SEQ ID NOs.: 3796-3800, 3806-4860, 5916-
10012, and 14111-14944 is underexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than

16773



strains which do not underexpress the gene product on which said compound
acts; and
identifying the gene product which is underexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

104. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism and wherein the nucleotide sequence of each of the
underexpressed genes has been altered so as to include a nucleotide sequence
which can be used to generate a unique product corresponding to each of the
overexpressed genes, wherein said culture comprises a strain in which a gene
product comprising an amino acid sequence selected from the group consisting
of SEQ ID NOs.: 3801-3805, 4861-5915, 10013-14110 and 14945-15778 is
underexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress the gene product on which said compound
acts; and
identifying the gene product which is underexpressed in a strain which
preliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

105. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism and wherein the nucleotide sequence of each of the
underexpressed genes has been altered so as to include a nucleotide sequence

16774



which can be used to generate a unique product corresponding to each of the
overexpressed genes, wherein said culture comprises a strain in which a gene
product selected from the group consisting of a gene product having at least
70%
nucleotide sequence identity as determined using BLASTN version 2.0 with the
default parameters to a gene product whose expression is inhibited by an
antisense nucleic acid comprising a nucleotide sequence selected from the
group
consisting of SEQ ID NOs.: 8-3795, a gene product encoded by a nucleic acid
having at least 70% nucleotide sequence identity as determined using BLASTN
version 2.0 with the default parameters to a nucleic acid encoding a gene
product
whose expression is inhibited by an antisense nucleic acid comprising a
nucleotide sequence selected from the group consisting of SEQ ID NOs: 8-3795,
a gene product having at least 25% amino acid identity as determined using
FASTA version 3.0t78 with the default parameters to a gene product whose
expression is inhabited by an antisense nucleic acid comprising a nucleotide
sequence selected from the group consisting of SEQ ID NOs.: 8-3795, a gene
product encoded by a nucleic acid which hybridizes to a nucleic acid
comprising
a nucleotide sequence selected from the group consisting of SEQ ID NOs.: 8-
3795 under stringent conditions, a gene product encoded by a nucleic acid
which
hybridizes to a nucleic acid comprising a nucleotide sequence selected from
the
group consisting of SEQ ID NOs.: 8-3795 under moderate conditions, and a
gene product whose activity may be complemented by the gene product whose
activity is inhibited by a nucleic acid comprising a nucleotide sequence
selected
from the group consisting of SEQ ID NOs: 8-3795 is underexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress the gene product on which said compound
acts; and

16775



identifying the gene product which is underexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

106. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism and wherein the nucleotide sequence of each of the
underexpressed genes has been altered so as to include a nucleotide sequence
which can be used to generate a unique product corresponding to each of the
overexpressed genes, wherein said culture comprises a strain in which a gene
product encoded by a nucleic acid comprising a nucleotide sequence selected
from the group consisting of a nucleic acid comprising a nucleic acid having
at
least 70% nucleotide sequence identity as determined using BLASTN version
2.0 with the default parameters to a nucleotide sequence selected from the
group
consisting of SEQ ID NOS.:3796-3800, 3806-4860, 5916-10012, and 14111-
14944, a nucleic acid comprising a nucleotide sequence which hybridizes to a
sequence selected from the group consisting of SEQ ID NOS.:3796-3800, 3806-
4860, 5916-10012, and 14111-14944 under stringent conditions, and a nucleic
acid comprising a nucleotide sequence which hybridizes to a nucleotide
sequence selected from the group consisting of SEQ ID NOS.:3796-3800, 3806-
4860, 5916-10012, and 14111-14944 under moderate conditions is
underexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress the gene product on which said compound
acts; and

16776


identifying the gene product which is underexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

107. A method for identifying the gene product on which a compound which
inhibits proliferation of an organism acts comprising:
obtaining a culture comprising a plurality of strains wherein each strain
underexpresses a different gene product which is essential for proliferation
of
said organism and wherein the nucleotide sequence of each of the
underexpressed genes has been altered so as to include a nucleotide sequence
which can be used to generate a unique product corresponding to each of the
overexpressed genes, wherein said culture comprises a strain in which a gene
product comprises a polypeptide selected from the group consisting of a
polypeptide having at least 25% amino acid identity as determined using
FASTA version 3.0t78 to a polypeptide selected from the group consisting of
SEQ ID NOs.:3801-3805, 4861-5915, 10013-14110 and 14945-15778 and a
polypeptide whose activity may be complemented by a polypeptide selected
from the group consisting of SEQ ID NOs:3801-3805, 4861-5915, 10013-
14110 and 14945-15778 is underexpressed;
contacting said culture with a sufficient concentration of said compound
to inhibit the proliferation of strains of said organism which underexpress
said
gene product on which said compound acts, such that strains which underexpress
said gene product on which said compound acts proliferate more slowly than
strains which do not underexpress the gene product on which said compound
acts; and
identifying the gene product which is underexpressed in a strain which
proliferated more rapidly in said culture by detecting the unique product
corresponding to said gene.

108. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a

16777


plurality of strains wherein each strain overexpresses or underexpresses a
different gene product which is required for proliferation of said organism;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the genes
which encode said gene products, wherein the members of said set of primer
pairs are designed such that each primer pair would yield an amplification
product having a length distinguishable from the lengths of the amplification
products from the other primer pairs if a strain comprising the nucleotide
sequences complementary to said primer pair is present in said culture or
collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction.

109. The method of Claim 108, wherein one member of each primer pair for
each of said genes is labeled with a detectable dye.

110. The method of Claim 108 wherein:
said nucleic acid sample is divided into N aliquots;
said amplification reaction is performed on each aliquot using primer
pairs complementary to nucleotide sequences within or adjacent to 1/N of the
genes which encode said gene products, wherein one of the members of each
primer pair in each aliquot is labeled with a dye and wherein the dyes on the
primers in each aliquot are distinguishable from one another.

111. The method of Claim 109, further comprising pooling the amplification
products from each of the aliquots prior to determining the lengths of the
amplification
products.

112. The method of Claim 108, wherein the native promoters of said genes
which encode said gene products have been replaced with a regulatable promoter
and
one of the primers in said primer pairs is complementary to a nucleotide
sequence
within said regulatable promoter.

113. The method of Claim 111, wherein the native promoters for each of said
genes were replaced with the same regulatable promoter.

16778


114. The method of Claim 111, wherein more than one regulatable promoter
was used to replace the promoters of said genes such that some of said genes
are under
the control of a different regulatable promoter.

115. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains wherein each strain overexpresses or underexpresses a
different gene product which is required for proliferation of said organism
wherein said culture comprises a strain in which a gene product whose activity
or level is inhibited by a nucleic acid comprising a nucleotide sequence
selected
from the group consisting of SEQ ID NOs.:8-3795 is overexpressed or
underexpressed;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the genes
which encode said gene products, wherein the members of said set of primer
pairs are designed such that each primer pair would yield an amplification
product having a length distinguishable from the lengths of the amplification
products from the other primer pairs if a strain comprising the nucleotide
sequences complementary to said primer pair is present in said culture or
collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction.

116. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains wherein each strain overexpresses or underexpresses a
different gene product which is required for proliferation of said organism,
wherein said culture comprises a strain in which a gene product encoded by a
nucleic acid comprising a nucleotide sequence selected from the group

16779


consisting of SEQ ID NOs.:3796-3800, 3806-4860, 5916-10012, and 14111-
14944 is overexpressed or underexpressed;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the genes
which encode said gene products, wherein the members of said set of primer
pairs are designed such that each primer pair would yield an amplification
product having a length distinguishable from the lengths of the amplification
products from the other primer pairs if a strain comprising the nucleotide
sequences complementary to said primer pair is present in said culture or
collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction.

117. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains wherein each strain overexpresses or underexpresses a
different gene product which is required for proliferation of said organism,
wherein said culture comprises a strain in which a gene product comprising an
amino acid sequence selected from the group consisting of SEQ ID NOs.:3801-
3805, 4861-5915, 10013-14110 and 14945-15778 is overexpressed or
underexpressed;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the genes
which encode said gene products, wherein the members of said set of primer
pairs are designed such that each primer pair would yield an amplification
product having a length distinguishable from the lengths of the amplification
products from the other primer pairs if a strain comprising the nucleotide
sequences complementary to said primer pair is present in said culture or
collection of strains; and

16780


determining the lengths of the amplification products obtained in said
amplification reaction.

118. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains wherein each strain overexpresses or underexpresses a
different gene product which is required for proliferation of said organism,
wherein said culture comprises a strain in which a gene product selected from
the group consisting of a gene product having at least 70% nucleotide sequence
identity as determined using BLASTN version 2.0 with the default parameters to
a gene product whose expression is inhibited by an antisense nucleic acid
comprising a nucleotide sequence selected from the group consisting of SEQ ID
NOs.:8-3795, a gene product encoded by a nucleic acid having at least 70%
nucleotide sequence identity as determined using BLASTN version 2,0 with the
default parameters to a nucleic acid encoding a gene product whose expression
is inhibited by an antisense nucleic acid comprising a nucleotide sequence
selected from the group consisting of SEQ ID NOs:8-3795, a gene product
having at least 25% amino acid identity as determined using FASTA version
3.0t78 with the default parameters to a gene product whose expression is
inhibited by an antisense nucleic acid comprising a nucleotide sequence
selected
from the group consisting of SEQ ID NOs.:8-3795, a gene product encoded by a
nucleic acid which hybridizes to a nucleic acid comprising a nucleotide
sequence
selected from the group consisting of SEQ ID NOs.:8-3795 under stringent
conditions, a gene product encoded by a nucleic acid which hybridizes to a
nucleic acid comprising a nucleotide sequence selected from the group
consisting of SEQ ID NOs.:8-3795 under moderate conditions, and a gene
product whose activity may be complemented by the gene product whose
activity is inhibited by a nucleic acid comprising a nucleotide sequence
selected
from the group consisting of SEQ ID NOs:8-3795 is overexpressed or
underexpressed;

16781


performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the genes
which encode said gene products, wherein the members of said set of primer
pairs are designed such that each primer pair would yield an amplification
product having a length distinguishable from the lengths of the amplification
products from the other primer pairs if a strain comprising the nucleotide
sequences complementary to said primer pair is present in said culture or
collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction.

119. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains wherein each strain overexpresses or underexpresses a
different gene product which is required for proliferation of said organism,
wherein said culture comprises a strain in which a gene product encoded by a
nucleic acid comprising a nucleotide sequence selected from the group
consisting of a nucleic acid comprising a nucleic acid having at least 70%
nucleotide sequence identity as determined using BLASTN version 2.0 with the
default parameters to a nucleotide sequence selected from the group consisting
of SEQ ID NOS.:3796-3800, 3806-4860, 5916-10012, and 14111-14944, a
nucleic acid comprising a nucleotide sequence which hybridizes to a sequence
selected from the group consisting of SEQ ID NOS.:3796-3800, 3806-4860,
5916-10012, and 14111-14944 under stringent conditions, and a nucleic acid
comprising a nucleotide sequence which hybridizes to a nucleotide sequence
selected from the group consisting of SEQ ID NOS.:3796-3800, 3806-4860,
5916-10012, and 14111-14944 under moderate conditions is overexpressed or
underexpressed;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the genes

16782


which encode said gene products, wherein the members of said set of primer
pairs are designed such that each primer pair would yield an amplification
product having a length distinguishable from the lengths of the amplification
products from the other primer pairs if a strain comprising the nucleotide
sequences complementary to said primer pair is present in said culture or
collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction.

120. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains wherein each strain overexpresses or underexpresses a
different gene product which is required for proliferation of said organism,
wherein said culture comprises a strain in which a gene product comprising a
polypeptide selected from the group consisting of a polypeptide having at
least
25% amino acid identity as determined using FASTA version 3.0t78 to a
polypeptide selected from the group consisting of SEQ ID NOs.:3801-3805,
4861-5915, 10013-14110 and 14945-15778 and a polypeptide whose activity
may be complemented by a polypeptide selected from the group consisting of
SEQ ID NOs: 3801-3805, 4861-5915, 10013-14110 and 14945-15778 is
overexpressed or underexpressed;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the genes
which encode said gene products, wherein the members of said set of primer
pairs are designed such that each primer pair would yield an amplification
product having a length distinguishable from the lengths of the amplification
products from the other primer pairs if a strain comprising the nucleotide
sequences complementary to said primer pair is present in said culture or
collection of strains; and

16783



determining the lengths of the amplification products obtained in said
amplification reaction.

121. A method far identifying the target of a compound which inhibits the
proliferation of an organism comprising:
obtaining a first nucleic acid sample comprising nucleic acids from a first
culture or collection of strains wherein said culture or collection of strains
comprises a plurality of strains wherein each strain overexpresses or
underexpresses a different gene product which is required for proliferation of
said organism and wherein said culture or collection of strains has been
contacted with said compound;
obtaining a second nucleic acid sample comprising nucleic acids from a
second culture or collection of strains wherein said culture or collection of
strains comprises the same strains as said first culture or collection of
strains
wherein said second culture or collection of strains has not been contacted
with
said compound;
performing a first amplification reaction on said first nucleic acid sample
using a set of primer pairs which are complementary to nucleotide sequences
within or adjacent to the genes which encode said gene products, wherein the
members of said set of primer pairs are designed such that each primer pair
would yield an amplification product having a length distinguishable from the
lengths of the amplification products from the other primer pairs if a strain
comprising the nucleotide sequences complementary to said primer pair is
present in said culture or collation of strains;
performing a second amplification reaction on said second nucleic acid
sample using the same set of primer pairs used in said first amplification
reaction;
and comparing the amount of each amplification product in said first
amplification reaction to the amount of that amplification product in said
second
amplification reaction, wherein an increased level of an amplification product
in
said first amplification reaction relative to said second amplification
reaction
indicates that the gene product corresponding to said amplification product is
the

16784



target of said compound if said culture or strain overexpresses said gene
products and a decreased level of of an amplification product in said first
amplification reaction relative to said second amplification reaction
indicates
that the gene product corresponding to said amplification product is the
target of
said compound if said culture or strain overexpresses said gene products.

122. The method of Claim 121, wherein one member of each primer pair for
each of said genes is labeled with a detectable dye.

123. The method of Claim 121, wherein the native promoters of said genes
which encode said gene products have been replaced with a regulatable promoter
and
one of the primers in said primer pairs is complementary to a nucleotide
sequence
within said regulatable promoter.

124. The method of Claim 121, wherein the native promoters for each of said
genes were replaced with the same regulatable promoter.

125. The method of Claim 121, wherein more than one regulatable promoter
was used to replace the promoters of said genes such that some of said genes
are under
the control of a different regulatable promoter.

126. A method for identifying the target of a compound which inhibits the
proliferation of an organism comprising:
obtaining a first nucleic acid sample comprising nucleic acids from a first
culture or collection of strains wherein said culture or collection of strains
comprises a plurality of strains wherein each strain overexpresses or
underexpresses a different gene product which is required for proliferation of
said organism and wherein said culture or collection of strains has been
contacted with said compound;
obtaining a second nucleic acid sample comprising nucleic acids from a
second culture or collection of strains wherein said culture or collection of
strains comprises the same strains as said first culture or collection of
strains
wherein said second culture or collection of strains has not beg contacted
with
said compound;
performing a first amplification reaction on said first nucleic acid sample
using a set of primer pairs which are complementary to nucleotide sequences

16785



within or adjacent to the genes which encode said gene products, wherein the
members of said set of primer pairs are designed such that each primer pair
would yield an amplification product having a length distinguishable from the
lengths of the amplification products from the other primer pairs if a strain
comprising the nucleotide sequences complementary to said primer pair is
present in said culture or collection of strains;
performing a second amplification reaction on said second nucleic acid
sample using the same set of primer pairs used in said first amplification
reaction;
and comparing the amount of each amplification product in said first
amplification reaction to the amount of that amplification product in said
second
amplification reaction, wherein an increased level of an amplification product
in
said first amplification reaction relative to said second amplification
reaction
indicates that the gene product corresponding to said amplification product is
the
target of said compound if said culture or strain overexpresses said gene
products and a decreased level of of an amplification product in said first
amplification reaction relative to said second amplification reaction
indicates
that the gene product corresponding to said amplification product is the
target of
said compound if said culture or strain overexpresses said gene products,
wherein said first and second cultures or collection of strains comprise a
strain in
which a gene product whose activity or level is inhibited by a nucleic acid
comprising a nucleotide sequence selected from the group consisting of SEQ ID
NOs.: 8-3795 is overexpressed or underexpressed.

127. A method for identifying the target of a compound which inhibits the
proliferation of an organism comprising:
obtaining a first nucleic acid sample comprising nucleic acids from a first
culture or collection of strains wherein said culture or collection of strains
comprises a plurality of strains wherein each strain overexpresses or
underexpresses a different gene product which is required for proliferation of
said organism and wherein said culture or collection of strains has been
contacted with said compound;

16786



obtaining a second nucleic acid sample comprising nucleic acids from a
second culture or collection of strains wherein said culture or collection of
strains comprises the same strains as said first culture or collection of
strains
wherein said second culture or collection of strains has not been contacted
with
said compound;
performing a first amplification reaction on said first nucleic acid sample
using a set of primer pairs which are complementary to nucleotide sequences
within or adjacent to the genes which encode said gene products, wherein the
members of said set of primer pairs are designed such that each primer pair
would yield an amplification product having a length distinguishable from the
lengths of the amplification products from the other primer pairs if a strain
comprising the nucleotide sequences complementary to said primer pair is
present in said culture or collection of strains;
performing a second amplification reaction on said second nucleic acid
sample using the same set of primer pairs used in said first amplification
reaction;
and comparing the amount of each amplification product in said first
amplification reaction to the amount of that amplification product in said
second
amplification reaction, wherein an increased level of an amplification product
in
said first amplification reaction relative to said second amplification
reaction
indicates that the gene product corresponding to said amplification product is
the
target of said compound if said culture or strain overexpresses said gene
products and a decreased level of of an amplification product in said first
amplification reaction relative to said second amplification reaction
indicates
that the gene product corresponding to said amplification product is the
target of
said compound if said culture or strain overexpresses said gene products,
wherein said first and second cultures or collection of strains comprise a
strain in
which a gene product encoded by a nucleic acid comprising a nucleotide
sequence selected from the group consisting of SEQ ID NOs.: 3796-3800, 3806-
4860, 5916-10012, and 14111-14944 is overexpressed or underexpressed.

16787


128. A method for identifying the target of a compound which inhibits the
proliferation of an organism comprising:
obtaining a first nucleic acid sample comprising nucleic acids from a first
culture or collection of strains wherein said culture or collection of strains
comprises a plurality of strains wherein each strain overexpresses or
underexpresses a different gene product which is required for proliferation of
said organism and wherein said culture or collection of strains has been
contacted with said compound;
obtaining a second nucleic acid sample comprising nucleic acids from a
second culture or collection of strains wherein said culture or collection of
strains comprises the same strains as said first culture or collection of
strains
wherein said second culture or collection of strains has not been contacted
with
said compound;
performing a first amplification reaction on said first nucleic acid sample
using a set of primer pairs which are complementary to nucleotide sequences
within or adjacent to the genes which encode said gene products, wherein the
members of said set of primer pairs are designed such that each primer pair
would yield an amplification product having a length distinguishable from the
lengths of the amplification products from the other primer pairs if a strain
comprising the nucleotide sequences complementary to said primer pair is
present in said culture or collection of strains;
performing a second amplification reaction on said second nucleic acid
sample using the same set of primer pairs used in said first amplification
reaction;
and comparing the amount of each amplification product in said first
amplification reaction to the amount of that amplification product in said
second
amplification reaction, wherein an increased level of an amplification product
in
said first amplification reaction relative to said second amplification
reaction
indicates that the gene product corresponding to said amplification product is
the
target of said compound if said culture or strain overexpresses said gene
products and a decreased level of of an amplification product in said first

16788





amplification reaction relative to said second amplification reaction
indicates
that the gene product corresponding to said amplification product is the
target of
said compound if said culture or strain overexpresses said gee products,
wherein said first and second cultures or collection of strains comprise a
strain in
which a gene product comprising an amino acid sequence selected from the
group consisting of SEQ ID NOs.: 3801-3805, 4861-5915, 10013-14110 and
14945-15778 is overexpressed or underexpressed.

129. A method for identifying the target of a compound which inhibits the
proliferation of an organism comprising:
obtaining a first nucleic acid sample comprising nucleic acids from a first
culture or collection of strains wherein said culture or collection of strains
comprises a plurality of strains wherein each strain overexpresses or
underexpresses a different gene product which is required for proliferation of
said organism and wherein said culture or collection of strains has been
contacted with said compound;
obtaining a second nucleic acid sample comprising nucleic acids from a
second culture or collection of strains wherein said culture or collection of
strains comprises the same strains as said first culture or collection of
strains
wherein said second culture or collection of strains has not been contacted
with
said compound;
performing a first amplification reaction on said first nucleic acid sample
using a set of primer pairs which are complementary to nucleotide sequences
within or adjacent to the gent which encode said gene products, wherein the
members of said set of primer pairs are designed such that each primer pair
would yield an amplification product having a length distinguishable from the
lengths of the amplification products from the other primer pairs if a strain
comprising the nucleotide sequences complementary to said primer pair is
present in said culture or collection of strains;
performing a second amplification reaction on said second nucleic acid
sample using the same set of primer pairs used in said first amplification
reaction;

16789




and comparing the amount of each amplification product in said first
amplification reaction to the amount of that amplification product in said
second
amplification reaction, wherein an increased level of an amplification product
in
said first amplification reaction relative to said second amplification
reaction
indicates that the gene product corresponding to said amplification product is
the
target of said compound if said culture or strain overexpresses said gene
products and a decreased level of of an amplification product in said first
amplification reaction relative to said second amplification reaction
indicates
that the gene product corresponding to said amplification product is the
target of
said compound if said culture or strain overexpresses said gene products,
wherein said first and second cultures or collection of strains comprise a
strain in
which a gene product selected from the group consisting of a gene product
having at least 70% nucleotide sequence identity as determined using BLASTN
version 2.0 with the default parameters to a gene product whose expression is
inhibited by an antisense nucleic acid comprising a nucleotide sequence
selected
from the group consisting of SEQ ID NOs.: 8-3795, a gene product encoded by a
nucleic acid having at least 70% nucleotide sequence identity as determined
using BLASTN version 2.0 with the default parameters to a nucleic acid
encoding a gene product whose expression is inhibited by an antisense nucleic
acid comprising a nucleotide sequence selected from the group consisting of
SEQ ID NOs: 8-3795, a gene product having at least 25% amino acid identity as
determined using FASTA version 3.0t78 with the default parameters to a gene
product whose expression is inhibited by an antisense nucleic acid comprising
a
nucleotide sequence selects from the group consisting of SEQ ID NOs.: 8-
3795, a gene product encoded by a nucleic acid which hybridizes to a nucleic
acid comprising a nucleotide sequence selected from the group consisting of
SEQ ID NOs.: 8-3795 under stringent conditions, a gene product encoded by a
nucleic acid which hybridizes to a nucleic acid comprising a nucleotide
sequence
selected from the group consisting of SEQ ID NOs.: 8-3795 under moderate
conditions, and a gene product whose activity may be complemented by the
gene product whose activity is inhibited by a nucleic acid comprising a

16790




nucleotide sequence selected from the group consisting of SEQ ID NOs: 8-3795
is overexpressed or underexpressed.

130. A method for identifying the target of a compound which inhibits the
proliferation of an organism comprising:
obtaining a first nucleic acid sample comprising nucleic acids from a fast
culture or collection of strains wherein said culture or collection of strains
comprises a plurality of strains wherein each strain overexpresses or
underexpresses a different gene product which is required for proliferation of
said organism and wherein said culture or collection of strains has been
contacted with said compound;
obtaining a second nucleic acid sample comprising nucleic acids from a
second culture or collection of strains wherein said culture or collection of
strains comprises the same strains as said first culture or collection of
strains
wherein said second culture or collection of strains has not been contacted
with
said compound;
performing a first amplification reaction on said first nucleic acid sample
using a set of primer pairs which are complementary to nucleotide sequences
within or adjacent to the genes which encode said gene products, wherein the
members of said set of primer pairs are designed such that each primer pair
would yield an amplification product having a Length distinguishable from the
lengths of the amplification products from the other primer pairs if a strain
comprising the nucleotide sequences complementary to said primer pair is
present in said culture or collection of strains;
performing a second amplification reaction on said second nucleic acid
sample using the same set of primer pairs used in said first amplification
reaction;
and comparing the amount of each amplification product in said first
amplification reaction to the amount of that amplification product in said
second
amplification reaction, wherein an increased level of an amplification product
in
said first amplification reaction relative to said second amplification
reaction
indicates that the gene product corresponding to said amplification product is
the

16791




target of said compound if said culture or strain overexpresses said gene
products and a decreased level of of an amplification product in said first
amplification reaction relative to said second amplification reaction
indicates
that the gene product corresponding to said amplification product is the
target of
said compound if said culture or strain overexpresses said gene products,
wherein said first and second cultures or collection of strains comprise a
strain in
which a gene product encoded by a nucleic acid comprising a nucleotide
sequence selected from the group consisting of a nucleic acid comprising a
nucleic acid having at least 70% nucleotide sequence identity as determined
using BLASTN version 2.0 with the default parameters to a nucleotide sequence
selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-4860,
5916-10012, and 14111-14944, a nucleic acid comprising a nucleotide sequence
which hybridizes to a sequence selected from the group consisting of SEQ ID
NOS.: 3796-3800, 3806-4860, 5916-10012, and 14111-14944 under stringent
conditions, and a nucleic acid comprising a nucleotide sequence which
hybridizes to a nucleotide sequence selected from the group consisting of SEQ
ID NOS.: 3796-3800, 3806-4860, 5916-10012, and 14111-14944 under
moderate conditions is overexpressed or underexpressed.

131. A method for identifying the target of a compound which inhibits the
proliferation of an organism comprising:
obtaining a first nucleic acid sample comprising nucleic acids from a first
culture or collection of strains wherein said culture or collection of strains
comprises a plurality of strains wherein each strain overexpresses or
underexpresses a different gene product which is required for proliferation of
said organism and wherein said culture or collection of strains has been
contacted with said compound;
obtaining a second nucleic acid sample comprising nucleic acids from a
second culture or collection of strains wherein said culture or collection of
strains comprises the same strains as said first culture or collection of
strains
wherein said second culture or collection of strains has not been contacted
with
said compound;

16792




performing a first amplification reaction on said first nucleic acid sample
using a set of primer pairs which are complementary to nucleotide sequences
within or adjacent to the genes which encode said gene products, wherein the
members of said set of primer pairs are designed such that each primer pair
would yield an amplification product having a length distinguishable from the
lengths of the amplification products from the other primer pairs if a strain
comprising the nucleotide sequences complementary to said primer pair is
present in said culture ar collection of strains;
performing a second amplification reaction on said second nucleic acid
sample using the same set of primer pairs used in said first amplification
reaction;
and comparing the amount of each amplification product in said first
amplification reaction to the amount of that amplification product in said
second
amplification reaction, wherein an increased level of an amplification product
in
said first amplification reaction relative to said second amplification
reaction
indicates that the gene product corresponding to said amplification product is
the
target of said compound if said culture or strain overexpresses said gene
products and a decreased level of of an amplification product in said first
amplification reaction relative to said second amplification reaction
indicates
that the gene product corresponding to said amplification product is the
target of
said compound if said culture or strain overexpresses said gene products,
wherein said first and second culture or collection of strains comprise a
strain in
which a gene product comprising a polypeptide selected from the group
consisting of a polypeptide having at least 25% amino acid identity as
determined using FASTA version 3.0t78 to a polypeptide selected from the
group consisting of SEQ ID NOs.: 3801-3805, 4861-5915, 10013-14110 and
14945-15778 and a polypeptide whose activity may be complemented by a
polypeptide selected from the group consisting of SEQ ID NOs: 3801-3805,
4861-5915, 10013-14110 and 14945-15778 is overexpressed or underexpressed.

132. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:

16793




obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which transcribe an antisense nucleic acid complementary
to
a different gene product which is required for proliferation of said organism;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the nucleic
acids which encode said antisense nucleic acids, wherein the members of said
set
of primer pairs are designed such that each primer pair would yield an
amplification product having a length distinguishable from the lengths of the
amplification products from the other primer pairs if a strain comprising the
nucleotide sequences complementary to said primer pair is present in said
culture or collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction.

133. The method of Claim 132, wherein one member of each primer pair for
each of said genes is labeled with a detectable dye.

134. The method of Claim 132 wherein:
said nucleic acid sample is divided into N aliquots;
said amplification reaction is performed on each aliquot using primer
pairs complementary to nucleotide sequences within or adjacent to 1/N of the
genes which encode said gene products, wherein one of the members of each
primer pair in each aliquot is labeled with a dye and wherein the dyes on the
primers in each aliquot are distinguishable from one another.

135. The method of Claim 134, further comprising pooling the amplification
products from each of the aliquots prior to determining the lengths of the
amplification
products.

136. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a

16794




plurality of strains which transcribe an antisense nucleic acid complementary
to
a different gene product which is required for proliferation of said organism;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the nucleic
acids which encode said antisense nucleic acids, wherein the members of said
set
of primer pairs are designed such that each primer pair would yield an
amplification product having a length distinguishable from the lengths of the
amplification products from the other primer pairs if a strain comprising the
nucleotide sequences complementary to said primer pair is present in said
culture or collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction, wherein said culture comprises a strain in which a
gene
product whose activity or level is inhibited by a nucleic acid comprising a
nucleotide sequence selected from the group consisting of SEQ ID NOs.: 8-3795
is overexpressed or underexpressed.

137. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which transcribe an antisense nucleic acid complementary
to
a different gene product which is required for proliferation of said organism;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the nucleic
acids which encode said antisense nucleic acids, wherein the members of said
set
of primer pairs are designed such that each primer pair would yield an
amplification product having a length distinguishable from the lengths of the
amplification products from the other primer pairs if a strain comprising the
nucleotide sequences complementary to said primer pair is present in said
culture or collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction, wherein said culture comprises a strain in which a
gene

16795




product encoded by a nucleic acid comprising a nucleotide sequence selected
from the group consisting of SEQ ID NOs.: 3796-3800, 3806-4860, 5916-
10012, and 14111-14944 is overexpressed or underexpressed.
138. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which transcribe an antisense nucleic acid complementary
to
a different gene product which is required for proliferation of said organism;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the nucleic
acids which encode said antisense nucleic acids, wherein the members of said
set
of primer pairs are designed such that each primer pair would yield an
amplification product having a length distinguishable from the lengths of the
amplification products from the other primer pairs if a strain comprising the
nucleotide sequences complementary to said primer pair is present in said
culture or collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction, wherein said culture comprises a strain in which a
gene
product comprising an amino acid sequence selected from the group consisting
of SEQ ID NOs.: 3801-3805, 4861-5915, 10013-14110 and 14945-15778 is
overexpressed or underexpressed.
139. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which transcribe an antisense nucleic acid complementary
to
a different gene product which is required for proliferation of said organism;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the nucleic
acids which encode said antisense nucleic acids, wherein the members of said
set

16796



of primer pairs are designed such that each primer pair would yield an
amplification product having a length distinguishable from the lengths of the
amplification products from the other primer pairs if a strain comprising the
nucleotide sequences complementary to said primer pair is present in said
culture or collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction, wherein said culture comprises a strain in which a
gene
product selected from the group consisting of a gene product having at least
70%
nucleotide sequence identity as determined using BLASTN version 2.0 with the
default parameters to a gene product whose expression is inhibited by an
antisense nucleic acid comprising a nucleotide sequence selected from the
group
consisting of SEQ ID NOs.: 8-3795, a gene product encoded by a nucleic acid
having at least 70% nucleotide sequence identity as determined using BLASTN
version 2.0 with the default parameters to a nucleic acid encoding a gene
product
whose expression is inhibited by an antisense nucleic acid comprising a
nucleotide sequence selected from the group consisting of SEQ ID NOs: 8-3795,
a gene product having at least 25% amino acid identity as determined using
FASTA version 3.0t78 with the default parameters to a gene product whose
expression is inhibited by an antisense nucleic acid comprising a nucleotide
sequence selected from the group consisting of SEQ ID NOs.: 8-3795, a gene
product encoded by a nucleic acid which hybridizes to a nucleic acid
comprising
a nucleotide sequence selected from the group consisting of SEQ ID NOs.: 8-
3795 under stringent conditions, a gene product encoded by a nucleic acid
which
hybridizes to a nucleic acid comprising a nucleotide sequence selected from
the
group consisting of SEQ ID NOs.: 8-3795 under moderate conditions, and a
gene product whose activity may be complemented by the gene product whose
activity is inhibited by a nucleic acid comprising a nucleotide sequence
selected
from the group consisting of SEQ ID NOs: 8-3795 is overexpressed or
underexpressed.
140. A method far determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
16797


obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which transcribe an antisense nucleic acid complementary
to
a different gene product which is required for proliferation of said organism;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the nucleic
acids which encode said antisense nucleic acids, wherein the members of said
set
of primer pairs are designed such that each primer pair would yield an
amplification product having a length distinguishable from the lengths of the
amplification products from the other primer pairs if a strain comprising the
nucleotide sequences complementary to said primer pair is present in said
culture or collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction, wherein said culture comprises a strain in which a
gene
product encoded by a nucleic acid comprising a nucleotide sequence selected
from the group consisting of a nucleic acid comprising a nucleic acid having
at
least 70% nucleotide sequence identity as determined using BLASTN version
2.0 with the default parameters to a nucleotide sequence selected from the
group
consisting of SEQ ID NOS.: 3786-3800, 3806-4850, 5916-10012, and 14111-
14944, a nucleic acid comprising a nucleotide sequence which hybridizes to a
sequence selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-
4860, 5916-10012, and 14111-14944 under stringent conditions, and a nucleic
acid comprising a nucleotide sequence which hybridizes to a nucleotide
sequence selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-
4850, 5916-10012, and 14111-14944 under moderate conditions is
overexpressed or underexpressed.
141. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
16798



plurality of strains which transcribe an antisense nucleic acid complementary
to
a different gene product which is required for proliferation of said organism;
performing an amplification reaction using a set of primer pairs which
are complementary to nucleotide sequences within or adjacent to the nucleic
acids which encode said antisense nucleic acids, wherein the members of said
set
of primer pairs are designed such that each primer pair would yield an
amplification product having a length distinguishable from the lengths of the
amplification products from the other primer pairs if a strain comprising the
nucleotide sequences complementary to said primer pair is present in said
culture or collection of strains; and
determining the lengths of the amplification products obtained in said
amplification reaction, wherein said culture comprises a strain in which a
gene
product comprising a polypeptide selected from the group consisting of a
polypeptide having at least 25% amino acid identity as determined using
FASTA version 3.0t78 to a polypeptide selected from the group consisting of
SEQ ID NOs.: 3801-3805, 4861-5925, 10013-14110 and 14945-15778 and a
polypeptide whose activity may be complemented by a polypeptide selected
from the group consisting of SEQ ID NOs: 3801-3805, 4861-5915, 10013-
14110 and 14945-15778 is overexpressed or underexpressed.
142. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which overexpress or underexpress a different gene
product
which is required for proliferation of said organism;
performing an amplification reaction using primer pairs which are
complementary to nucleotide sequences within or adjacent to the genes which
encode said gene products, wherein said primer pairs are designed such that
each
primer pair would yield an amplification product which is distinguishable from
the amplification products produced by the other primer pairs on the a basis
selected from the group consisting of length, detectable label and both length
16799




and detectable label if a strain comprising the nucleotide sequences
complementary to said primer pair is present in said culture or collection of
strains; and
identifying the amplification products obtained in said amplification
reaction.
143. The method of Claim 142, wherein said primer pairs are divided into at
least two sets, each primer pair comprises a primer which is labeled with a
distinguishable dye, and the distinguishable dye used to label each set of
primer pairs is
distinguishable from the dye used to label the other sets of primer pairs.
144. The method of Claim 142 wherein:
said nucleic acid sample is divided into N aliquots;
said amplification reaction is performed on each aliquot using primer
pairs complementary to nucleotide sequences within or adjacent to 1/N of the
genes which encode said gene products, wherein one of the members of each
primer pair in each aliquot is labeled with a dye and wherein the dyers on the
primers in each aliquot are distinguishable from one another.
145. The method of Claim 144, further comprising pooling the amplification
products from each of the aliquots prior to determining the lengths of the
amplification
products.
146. The method of Claim 142, wherein the native promoters of said genes
which encode said gene products have been replaced with a regulatable promoter
and
one of the primers in said primer pairs is complementary to a nucleotide
sequence
within said regulatable promoter.
147. The method of Claim 146, wherein the native promoters for each of said
genes were replaced with the same regulatable promoter.
148. The method of Claim 146, wherein more than one regulatable promoter
was used to replace the promoters of said genes such that some of said genes
are under
the control of a different regulatable promoter.
149. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
16800




obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which overexpress or underexpress a different gene
product
which is required for proliferation of said organism;
performing an amplification reaction using primer pairs which are
complementary to nucleotide sequences within or adjacent to the genes which
encode said gene products, wherein said primer pairs are designed such that
each
primer pair would yield an amplification product which is distinguishable from
the amplification products produced by the other primer pairs on the a basis
selected from the group consisting of length, detectable label and both length
and detectable label if a strain comprising the nucleotide sequences
complementary to said primer pair is present in said culture or collection of
strains; and
identifying the amplification products obtained in said amplification
reaction, wherein said culture comprises a strain in which a gene product
whose
activity or level is inhibited by a nucleic acid comprising a nucleotide
sequence
selected from the group consisting of SEQ ID NOs.: 8-3795 is overexpressed or
underexpressed.
150. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which overexpress or underexpress a different gene
product
which is required for proliferation of said organism;
performing an amplification reaction using primer pairs which are
complementary to nucleotide sequences within or adjacent to the genes which
encode said gene products, wherein said primer pairs are designed such that
each
primer pair would yield an amplification product which is distinguishable from
the amplification products produced by the other primer pairs on the a basis
selected from the group consisting of length, detectable label and both length
and detectable label if a strain comprising the nucleotide sequences
16801


complementary to said primer pair is present in said culture or collection of
strains; and
identifying the amplification products obtained in said amplification
reaction, wherein said culture comprises a strain in which a gene product
encoded by a nucleic acid comprising a nucleotide sequence selected from the
group consisting of SEQ ID NOs.: 3796-3800, 3806-4860, 5916-10012, and
14111-14944 is overexpressed or underexpressed.
151. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
ar collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which overexpress or underexpress a different gene
product
which is required for proliferation of said organism;
performing an amplification reaction using primer pairs which are
complementary to nucleotide sequences within or adjacent to the genes which
encode said gene products, wherein said primer pairs are designed such that
each
primer pair would yield an amplification product which is distinguishable from
the amplification products produced by the other primer pairs on the a basis
selected from the group consisting of length, detectable label and both length
and detectable label if a strain comprising the nucleotide sequences
complementary to said primer pair is present in said culture or collection of
strains; and
identifying the amplification products obtained in said amplification
reaction, wherein said culture comprises a strain in which a gene product
comprising an amino acid sequence selected from the group consisting of SEQ
ID NOs.: 3801-3805, 4861-5915, 10013-14110 and 14945-15778 is
overexpressed or underexpressed.
152. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
16802


plurality of strains which overexpress or underexpress a different gene
product
which is required for proliferation of said organism;
performing an amplification reaction using primer pairs which are
complementary to nucleotide sequences within or adjacent to the genes which
encode said gene products, wherein said primer pairs are designed such that
each
primer pair would yield an amplification product which is distinguishable from
the amplification products produced by the other primer pairs on the a basis
selected from the group consisting of length, detectable label and both length
and detectable label if a strain comprising the nucleotide sequences
complementary to said primer pair is present in said culture or collection of
strains; and
identifying the amplification products obtained in said amplification
reaction, wherein said culture comprises a strain in which a gene product
selected from the group consisting of a gene product having at least 70%
nucleotide sequence identity as determined using BLASTN version 2.0 with the
default parameters to a gent product whose expression is inhibited by an
antisense nucleic acid comprising a nucleotide sequence selected firm the
group
consisting of SEQ ID NOs.: 8-3795, a gene product encoded by a nucleic acid
having at least 70% nucleotide sequence identity as determined using BLASTN
version 2.0 with the default parameters to a nucleic acid encoding a gene
product
whose expression is inhibited by an antisense nucleic acid comprising a
nucleotide sequence selected from the group consisting of SEQ ID NOs: 8-3795,
a gene product having at least 25% amino acid identity as determined using
FASTA version 3.0t78 with the default parameters to a gene product whose
expression is inhibited by an antisense nucleic acid comprising a nucleotide
sequence selected from the group consisting of SEQ ID NOs.: 8-3795, a gene
product encoded by a nucleic acid which hybridizes to a nucleic acid
comprising
a nucleotide sequence selected from the group consisting of SEQ ID NOs.: 8-
3795 under stringent conditions, a gene product encoded by a nucleic acid
which
hybridizes to a nucleic acid comprising a nucleotide sequence selected from
the
group consisting of SEQ ID NOs.: 8-3795 under moderate conditions, and a
16803



gene product whose activity may be complemented by the gene product whose
activity is inhibited by a nucleic acid comprising a nucleotide sequence
selected
from the group consisting of SEQ ID NOs: 8-3795 is overexpressed or
underexpressed.

153. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which overexpress or underexpress a different gene
product
which is required for proliferation of said organism;
performing an amplification reaction using primer pairs which are
complementary to nucleotide sequences within or adjacent to the genes which
encode said gene products, wherein said primer pairs are designed such that
each
primer pair would yield an amplification product which is distinguishable from
the amplification products produced by the other primer pairs on the a basis
selected from the group consisting of length, detectable label and both length
and detectable label if a strain comprising the nucleotide sequences
complementary to said primer pair is present in said culture or collection of
strains; and
identifying the amplification products obtained in said amplification
reaction, wherein said culture comprises a strain in which a gene product
encoded by a nucleic acid comprising a nucleotide sequence selected from the
group consisting of a nucleic acid comprising a nucleic acid having at least
70%
nucleotide sequence identity as determined using BLASTN version 2.0 with the
default parameters to a nucleotide sequence selected from the group consisting
of SEQ ID NOS.: 3796-3800, 3806-4860, 5916-10012, and 14111-14944, a
nucleic acid comprising a nucleotide sequence which hybridizes to a sequence
selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-4860,
5916-10012, and 14111-14944 under stringent conditions, and a nucleic acid
comprising a nucleotide sequence which hybridizes to a nucleotide sequence
selected from the group consisting of SEQ ID NOS.: 3796-3800, 3806-4860,

16804




5916-30012, and 14111-14944 under moderate conditions is overexpressed or
underexpressed.
154. A method for determining the extent to which each of a plurality of
strains are present in a culture or collection of strains comprising:
obtaining a nucleic acid sample comprising nucleic acids from a culture
or collection of strains wherein said culture or collection of strains
comprises a
plurality of strains which overexpress or underexpress a different gene
product
which is required for proliferation of said organism;
performing an amplification reaction using primer pairs which are
complementary to nucleotide sequences within or adjacent to the genes which
encode said gene products, wherein said primer pairs are designed such that
each
primer pair would yield an amplification product which is distinguishable from
the amplification products produced by the other primer pairs on the a basis
selected from the group consisting of length, detectable label and both length
and detectable label if a strain comprising the nucleotide sequences
complementary to said primer pair is present in said culture or collection of
strains; and
identifying the amplification products obtained in said amplification
reaction, wherein said culture comprises a strain in which a gene product
comprising a polypeptide selected from the group consisting of a polypeptide
having at least 25% amino acid identity as determined using FASTA version
3.0t78 to a polypeptide selected from the group consisting of SEQ ID NOs.:
3801-3805, 4861-5915, 10013-14110 and 14945-15778 and a polypeptide
whose activity may be complemented by a polypeptide selected from the group
consisting of SEQ ID NOs: 3801-3805, 4851-5915, 10013-14110 and 14945-
15778 is overexpressed or underexpressed.
16805

Description

CA 02437537 2003-08-27
1
Background of Invention:
Field of the Invention:
The invention relates generally to the field of computer. More specifically,
the invention
relates to docking mechanism between a touch tablet computer device and a
laptop computer. The
touch tablet computer device, henceforth referred to as 'tablet', has been on
the market for some
time. Tablet designs vary between specialized tablets, such as those used by
courier companies to
acquire recipient signatures, to those like the hand-held Fujitsu Stylistic
Tablet PC TM which cater to
general-purpose computing. Tablets fulfill the requirement for portability and
encourage the
purchase of extra electronic devices to satisfy specific requirements. The
proliferation of the PDAs
l0 (Personal Digital Assistants) over the last seven years is analogous to the
rise of calculators over the
last 30 years - people are buying them for their ability and portability.
Understandably, users will occasionally want a full feature computer to do
more complex
tasks. Although a laptop is not as portable as a tablet, it is a very popular
choice for complex tasks as
demonstrated by the marketplace. According to a study by IDCTM, PC
manufacturers shipped
approximately 30.5 million laptops worldwide in 2002. In doing so, individuals
who have purchased
laptops and now wish to acquire the features of a tablet are forced to
purchase tablet only devices. In
turn, an opportunity has arisen for designs that enable the conversion of
these laptops into 'tablet
capable machines'. A new design is needed that offers the features of a
lightweight tablet, laptop
computing power when needed and is able to utilize the abundant supply of
laptops worldwide.
2o Description of prior art
European patent US2002/011375 depicts a design whereby a tablet combines with
another of
the same to form a larger unit. Unfortunately, the system is awkward to use.
European patent EP1227387 details a design of a notebook computer with a
detachable
display. Novel in concept, it lacks in ability because the display only relays
computer information
and does not house a computer processor.
US patent 5,355,279 depicts a display design that is removable to allow the
display to reverse.
Again, the design does not permit any function other than display information
from the main laptop
unit.
US patent 5,796,576 allows a display to slide out of a laptop computer unit
but does not
3o provide any computing power on the display.


CA 02437537 2003-08-27
2
US design patent D421,972 illustrates a laptop computer capable of detaching
its keyboard to
allow the remainder portion to form an upright display unit. Unfortunately,
the unit does not form a
stand-alone tablet.
European patent FR2?99016 details a portable rigid frame that houses a
keyboard and a
35 display terminal. The keyboard is detached from the main unit while the
rest of the unit is used as a
display terminal. Here a separate unit is formed, but the design for the
display unit does not function
as a stand-alone tablet.
US patent 6,166,734 and similarly, Microsoft MiraTM, offer a computer display
that connects
wirelessly to a host computer
(http://www.microsoft.coralwindowslsmartdisplay/evaluation/faq.asp.)
4o However, they only relay the information and do not process the display
information.
The current tablet designs are more or less laptops that swivel their displays
to change into
tablet computers. Unfortunately, these designs typically weigh three pounds or
more and make
portability a major inconvenience for the user - they are full computers
miniaturized into a small
tablet form factor. Their processors and hard drives consume large amounts of
battery power and
45 generate a lot of heat. Furthermore, they run large operating systems, such
as Microsoft Tablet
PCTM, which demand large amounts of memory to function. It is obvious that the
prior art fails to
address: (1) the issue of portability and (2) allow the user the flexibility
to perform high
computational tasks when required.
Summary of the Invention
5o The invention pertains to a new way of combining the computing power of a
laptop and the
portability of a tablet.
The new computer product is a two-part docking mechanism that allows a tablet
to dock onto
a 'Laptop-Base Computer' (LBC) to form a portable computer called a 'Notebook-
Tablet'. The
Notebook-Tablet is comprised of (1) a LBC and (2) a tablet. The LBC is a
regular laptop computer
55 without the display screen - the video information still exists internally
within the LBC, however, an
external monitor must be attached to the LBC's video port to view the video
information. The tablet
is a standalone lightweight device built with a simple CPU (as compared to the
LBC CPU) and
when docked onto a LBC it changes function from a tablet to a LBC display.
The docking mechanism is comprised of two components: (1) a docking-cover and
(2) an
60 ejector plate. The docking-cover supports the tablet when the user angles
the display in notebook
configuration. Also, the shape of the docking-cover helps move the tablet 'in
and out' of docking
position with ease. When the tablet is no longer docked, the docking-cover
folds down to cover the


CA 02437537 2003-08-27
3
keyboard. The ejector plate secures the tablet in the docked configuration and
pushes out the tablet
when the tablet is removed for independent use.
65 The docking-cover design fits most current laptop models with only some
modification required
to the laptop lid - the laptop inner components and peripherals can be used
without modification.
The simple design encourages the quick introduction of the Notebook-Tablet to
the market and in
turn, the commerce associated with it. The docking-cover design allows laptop
manufacturers to
convert existing laptop designs into Notebook-Tablet designs and still address
the retrofit market for
'7o the number of laptops already in existence. In both cases, the conversion
is facilitated by: (1)
replacing the top part of the laptop casing with a docking-cover and an
ejector plate and (2)
connecting the display signal ribbon, USB (Universal Serial Bus) connector and
DC power to the
new docking-cover design. The components required to implement the change are
illustrated in
Figure 2.
~s Brief description of the drawings
Figure 1. Isometric view of the Notebook-Tablet in ejecting configuration with
an enlarged view of
the connector assembly at A. All ports and peripheral slots on the laptop
processor unit are
omitted for clarity.
Figure 2. Three components that need modification to convert a laptop computer
into a Notebook-
80 Tablet computer. The keyboard on the laptop processor unit is omitted for
clarity.
Figure 3a. Cross section of the Notebook-Tablet in Configuration 1 - Docked
and Closed.
Figure 3b. Cross section of the Notebook-Tablet in Configuration 2 - Docked
and Opened.
Figure 3c. Cross section of the Notebook-Tablet in Configuration 3 - Ejecting.
Figure 4. Isometric view of the Notebook-Tablet in the 'Docked and Closed'
position.
85 Figure 5. Isometric view of the Notebook-Tablet in the 'Docked and Opened'
position.
Figure 6. Isometric view of the LBC with the docking-cover closed to protect
the keyboard.
Figure 7. Four profile views of the Notebook-Tablet in the 'Docked and Closed'
configuration.
Counter clockwise from top left: top view, front view, right side view, and
isometric view.
Figure 8. Four profile views of the LBC with the tablet removed and the
docking-cover opened.
9o Counter clockwise from top left: top view, front view, right side view, and
isometric view.
Figure 9. Four profile views of the LBC with the tablet removed and the
docking-cover closed to
protect the keyboard. Counter clockwise from top left: top view, front view,
right side view,
and isometric view.
Figure 10a. Schematic views of the information flow when the tablet is docked
onto the LBC. The
95 tablet's display signal comes from the video connector while tablet's
memory and
peripherals are accessed through the USB connector.
Figure lOb. Schematic views of the information flow when the tablet is ejected
from the LBC.


CA 02437537 2003-08-27
4
Figure 11. The tablet lock mechanism with the lock in the open position. The
view is from the back
(bottom right) of the Notebook Tablet looking towards the front (top left) of
the Notebook
10o Tablet.
Figure 12. Alternate locations for tablet lock.
Figure 13. Alternate locations for ejector plate.
Parts - Numbers and Names
Number Name


105 Whole unit Notebook Tablet ,


Laptop-Base Computer (LBC)


Width dimension (also side direction)


Length dimension (also front direction)


Height dimension (also top direction)


ll0 100 Tablet


101 Expansion slot


102 Lock hole


103 Tablet pen


200 Docking-cover


115 201 Side-guides


202 Connector assembly


203 Guide pins


204 Video connector


205 USB connector


120 206 DC power connector


207 Guide pin lock


208 Tablet lock


209a Alternate top surface lock location


209b Alternate side lock locations


125 210a Alternate top ejector plate location


210b Alternate side ejector plate locations


220 Docking-cover end


300 Ejector plate


301 Lock notches


130 302 Rocker arms


400 Laptop processor unit


401 Hinges


402a Wire ribbon connecting laptop processor unit and underside
of docking-cover


402b Wire ribbon connecting laptop processor unit and docking-cover
through hinge


135 403 Line of pivot


Detailed description of the Invention
Figure 1 illustrates the separate components of the invention. The entire unit
is referred to as a
'Notebook-Tablet'. With the tablet [100] removed, the remaining unit is
referred to as the Laptop-
Base Computer (LBC) [10]. The LBC contains a docking-cover [200] that acts as
a holder for the
140 tablet and as a cover for the keyboard when the tablet is removed. At the
bottom of the docking-


CA 02437537 2003-08-27
cover is the ejector plate [300] that keeps the tablet in place during docked
configuration and pushes
out the tablet when the user wants to eject the tablet from the docking-cover.
The docking-cover,
along with the ejector plate, connect to the laptop processor unit via hinges
[401] on the laptop
processor unit [400]. The laptop processor unit is the box unit that houses
the main CPU, memory
145 and all the motherboard components that perform the function of a regular
laptop. It also houses all
built-in ports and peripheral slots required for operation as a regular
laptop. All the ports and
peripheral slots on the laptop processor unit are omitted for illustration
clarity. Toward the bottom of
the docking-cover is the connector assembly [202]. The connector assembly is
equipped with three
electrical connections: a male video connector [204], a male USB connector
[205] and a male DC
15o power connector [206]. By separating the connector assembly into three
parts, the tablet easily
integrates into existing laptop designs with minimal modification. The video
connector facilitates
video transmissions to the tablet display. The USB connector allows
information to flow freely
between the LBC and the tablet. And lastly, the power connector provides a
charging source for the
tablet battery. When the LBC is not plugged into a DC power source, the LBC
and in turn the
155 tablet, are unable to recharge. The connector assembly is enlarged for
clarity in Figure 1.
Inside the docking-cover, at the bottom of the connector assembly, sits the
electronic system
that connects the laptop processor unit to the tablet. The electronic system
is hidden inside the
docking-cover. The connection between the laptop processor unit and the
docking-cover can be
configured in one of two ways: (1) hidden within one of the hinges [402b] or
(2) inconspicuously
160 wired underneath the docking-cover [402a].
To further detail the mechanics and configurations of the unit, it is most
favourable to define a
common point of reference. With the Notebook-Tablet in front of the user and
the line of pivot
[403] running parallel to the user's chest, the dimension parallel to the line
of pivot and the user's
chest is defined as width [20]. The dimension perpendicular to the width and
directed toward the
165 user is defined as length [30]. The dimension perpendicular to both the
width and length is defined
as height [40]. The location closest to the user is the front [30] and the
location furthest from the
user is the back. The left and right sides of the user correspond to the left
and right sides [20] of all
the components, while the upward orientation is the top [40] and downward
orientation is the
bottom.
170 There are three configurations for the Notebook-Tablet: (1) Docked and
Closed, (2) Docked and
Opened and (3) Ejecting and Docking. Figures 1 to 6 illustrate the operation
of the Notebook-Tablet.
Configuration 1 - Docked and Closed: When the tablet is docked onto the LBC
and is closed,
the Notebook-Tablet resembles a laptop computer as shown in Figure 4. In the
docked and closed


CA 02437537 2003-08-27
6
position, the width of the laptop processor unit is the same as the width of
the tablet. The width of
n75 the docking cover is the same as the width of the laptop processor unit
plus the thickness of the two
side-guides [201 ], so that the docking-cover can fold down and have the side-
guides fit snuggly
against the left and right sides of the laptop processor unit as illustrated
in Figure 9. The length of
the tablet is slightly less than the length of the laptop processor unit to
ensure that when docked and
closed, the front edge of the tablet matches the front edge of the laptop
processor unit. The length of
180 the hollowed out portion of the docking-cover, where the tablet sits when
docked, is equal to half the
length of the tablet. The remaining half of the tablet protrudes beyond the
docking-cover.
The tablet is kept in position via two lock notches [301] on the ejector plate
that latch onto two
corresponding lock holes [102] on the tablet as illustrated in Figure 3a. If
so desired, an optional
tablet lock [208] can be fitted to permanently secure the tablet onto the
docking-cover. The side-
185 guides on the docking-cover block half of the tablet expansion slot [101]
to minimize the potential
for theft of expansion cards, and at the same time, accommodate wireless card
antennas or similar
while docked onto the LBC. Further details of the top, front, right, and
isometric views of the
Notebook-Tablet are shown in Figure 7.
Configuration 2 - Docked and Opened: When the tablet is docked and opened (as
illustrated
19o in Figure 5), the Notebook-Tablet functions as a regular laptop computer.
In this configuration, the
tablet operates as a display for the laptop processor unit. The video signals
from the laptop processor
unit pass-through to the tablet via the connector assembly's video connector.
Aside from sending
video signals, the LBC can share expansion features from tablet add-on cards.
As illustrated in
Figure 10a, whenever the tablet is docked, the tablet shares information with
the LBC. For example,
195 the laptop processor unit can access a digital camera card connected to
the tablet when the tablet is
in docked formation. The information is transferred through the USB connector
on the connector
assembly. In this position, the LBC can retrieve information stored on the
tablet and upload
information to the tablet.
The ejector plate, when in the docked and opened position, holds the tablet in
place with its
20o two lock notches as shown in Figure 3b. While assisting the ejector plate
to secure the tablet in
place, the connector assembly and the flat surface of the docking-cover also
allow the tablet to pivot
open and close along the line of pivot [403] in the same fashion as a laptop
display.
Configuration 3 - Ejecting and Docking: To disengage the tablet from the LBC,
the user pulls
back on the ejector plate toward the front of the LBC. The rotating motion of
the ejector plate pulls
205 the lock notches away from the lock holes on the tablet with the two side-
guides on the docking-
cover allowing the tablet to move out in a straight path. At the same time,
the rocker arms [302] on


CA 02437537 2003-08-27
7
the ejector plate push the tablet upward as illustrated in Figure 3c. Once the
tablet is about 2 to 3
mm above its docking position, the user can pull the tablet out of the docking-
cover and use it as a
portable device. The removal of the tablet from the LBC reveals the tablet pen
[103] tucked in
2~o behind the ejector plate. Figure 8 shows four profile views-top, front,
right and isometric-of the
LBC while the docking-cover is opened and the tablet is removed.
Once the tablet is removed from the docking-cover, the docking-cover folds
down to cover the
keyboard as shown in Figures 6 and 9. With the tablet removed, the CPU in the
laptop processor
functions independently of the tablet. In this state, the tablet accepts user
input, runs small programs
215 and displays information on its large touch screen. When either docked or
removed, the tablet
operates under its own power source-NiMH, LiIon or future fuel cell batteries.
To conserve power,
it is not equipped with a speaker, but might house an audio input/output jack
for convenience.
Figure lOb illustrates the flow of information when the tablet is removed from
the docking-cover.
To dock the tablet, the process is reversed. The user slides the tablet into
the docking-cover.
22o As the tablet slides down toward the connector assembly, the side-guides
keep the tablet aligned to
meet to the connector assembly. The guide pin holes on the tablet slide into
the guide pins [203] on
the docking-cover and eventually, the bottom of the tablet contacts the rocker
arms of the ejector
plate. As the tablet pushes the rocker arms downward, the ejector plate pivots
and moves the lock
notches toward the lock holes on the tablet. When the tablet comes to rest at
the bottom of the
225 docking-cover, the lock notches are nicely nestled into the tablet's lock
holes and the connector
assembly is in complete contact with the corresponding connections on the
tablet.
Other potential locations for the ejector plate include: (1) the top of the
docking-cover [210a]
and (2) both sides of the docking-cover (210b]. Both options are as
illustrated in Figure 13. These
alternatives are advantageous because they provide high visibility. However,
to protect against
230 inappropriate tampering, the ejector plate would need to be reinforced.
An optional locking mechanism is available for the Notebook Tablet. It is a
shaft that extends
from the lock's cylinder into the right side guide pin, as illustrated in
Figure 11. In the open position,
the tablet is able to freely slide 'in and out' of the guide pin lock without
obstruction. The tablet is
locked by rotating the guide pin [203] 90 degrees so that the guide pin lock
[207] mates with the
235 lock hole [102]. The lock is located on the docking-cover end [220] with
its key entry portion facing
the back of the docking-cover as illustrated in Figure 11. Alternative tablet
lock locations--( 1 ) at
the top of the docking-cover [209a] or (2) at one of the sides of the docking-
cover [209b]-are
illustrated in Figure 12 that provide good visibility, easy access and allow
the user to lock the tablet


CA 02437537 2003-08-27
in either the open or closed position. The tablet lock can also be located to
work in conjunction with
240 the ejector plate or independent of the ejector plate.
Although the invention is described in connection with a preferred embodiment,
it should be
understood that various modifications, additions and alterations may be made
to the invention by
one skilled in the art without departing from the spirit and scope of the
invention as defined in the
appended claims.
245

Representative Drawing