Note : Les descriptions sont présentées dans la langue officielle dans laquelle elles ont été soumises.
--`1 07~ZO9
The pre~ent invention i~ concerned with new qll~noline
carbo~ylic acid derivatives ha~ing ~aluable pharmacological
properties as urlnary a~ti~eptic~, with a proce~ for their
m3nuiacture and with ~heir use.
~he present i~vention provides ql7inoline carbo~ylic acid
derivative~ o~ the general ~ormula I .
~ coo~2 (I)
N
:
in which
Rl represents an alkenyl group containing 2 to 4 carbon atoms
and an alkynyl group containing 2 to 4 carbon atoms, and
R2 represents hydrogen; an alkenyl group containins 2 to 4
carbon atoms or an alkynyl group containing 2 to 4 carbon atoms
and also, when R2 represents a hydrogen atom, physiologically
tolerable salts thereof with inorganic or organic bases.
As the aforesaid straight chained or branched alkenyl or
alkynyl groups, there may be mentioned, for example, vinyl,
allyl, l-propenyl, l-butenyl, 2-butenyl, propadienyl, butadienyl,
isopropenyl, l-methylpropenyl, 2-methylpropenyl, 2-methylallyl,
3,3-dimethylallyl, ethynyl, propargyl, butynyl, and 1-methyl-2-
propynyl groups.
As the aforesaid straight chained or branched saturated
alkyl groups, there may be mentioned, for example, methyl, ethyl,
propyl, isopropyl, butyl, sec.-butyl, tert.-butyl and isobutyl.
~ .
~07~Z09
When R2 represents a hydrogen atom, the new compounds
may be used in the form of physiologically tolerable water-
soluble salts thereof with inorganic or organic bases.
For salt formation there comes into consideration
inorganic and organic bases of the type that are customarily
used by the expert in the art.
As bases that are preferably used in a known manner for
salt formation, there comes into consideration, for example,
potassium hydroxide, sodium hydroxide, lithium hydroxide,
calcium hydroxide, magnesium hydroxide, glucamine, N-methyl-
glucamine, N,N-dimethylglucamine, ethanolamine, diethanolamine,
morpholine, N-methylmorpholine and tris-(hydroxymethyl)-
methylamine.
From U.S. Patent No. 3,773,769, it has been known that
2,3-dihydrofuro[2,3-g]quinoline-7-carboxylic acids exhibit good
inhibiting values against gram-positive and gram-negative
bacteria and are thus suitable as urinary tract therapeutical
agents. Compounds for this indication must exhibit an adequate
elimination via the urine, because the dosage depends on this.
Only with an increase in the urine level can the administration
of an active substance be effected in a lower dosage.
It has now been found that the new compounds of the
general formula I coupled with a good inhibiting action against
gram-positive and gram-negative bacteria exhibit as compared,
for example, with 5-ethyl-2,3,5,8-tetrahydro-8-oxo-furo[2,3-g]-
quinoline-7-carboxylic acid (of U.S. Patent No. 3,773,769), an
increased urine elimination, as is shown in the following
Table by way of example with reference to the compounds of
Example 3 and Example 4 as compared with 5-ethyl-2,3,5,8-
tetrahydro-8-oxo-furo[2,3-g]quinoline-7-carboxylic acid. The
numerical values give the percentage content of an administered
dose which is eliminated in the urine during the course of 6
hours in the case of rats at a dosage of 100 mg/kg ~ os.
3 --
. . ~ .
llD7~0~
5-Ethyl-2,3,5,8- 8-Oxo-5-(1- 8-Oxo-2,3,5,8-
tetrahydro-8-oxo- propenyl)- tetrahydro-5-
Compound furo[2,3-g]- 2,3,5,8- vinyl-furo-
quinoline-7-car- tetrahydro- [2,3-g]quino-
boxylic acid furo-[2,3-g~ line-7-car-
quinoline-7- boxylic acid
carboxylic
. (Example 3) (Example 4)
. _
Elimination (%) 0.48% 2.1% 3.4%
The new compounds may be administered in the pharma-
ceutically usualforms ofapplications for example tablets, dragees,
capsules, pills, solutions and suspensions. ~
The present invention also provides a process for the
manufacture of a compound of the general formula I, or a physio-
logically tolerable salt with an inorganic or organic base of
such a compound in which R2 represents a hydrogen atom, wherein
a compound of the general formula II, which may be in the tauto-
meric form of the general formula II A or II B,
OH
~ COOR2 ~ COOR2
. II A II B
in which R2 has the meaning given above, is reacted with a com-
. pound of the general formula R"X in which: R" has the meaning
given above ~or Rl or represents an alkyl group containing 1 to
4 carbon atoms which may be straight chained or branched and
~ which is substituted by 1 or 2 halogen atoms, and X represents
., .
a halogen atom, an alkane-sulphonyloxy group containing 1 to 4
carbon atoms in the alkyl group or an aryl-sulphonyloxy group
(especially a benzene-sulphonyloxy group)
:,
-" 1071Z09
which may be substituted in the aryl part by one or more
substituents selected from halogen atoms and alkyl groups
containing 1 to 4 carbon atoms,
and, when R" represents a halogen substituted alkyl group, the
halo substituent on this group is/are split off to form an
unsaturated hydrocarbon group, and then, if desired, in any order
of succession, in the resulting compound any free carboxyl group
is esterified and/or any esterified carboxyl group is hydrolysed
and/or a carbon-to-carbon multiple bond in the group represented
by Rl is rearranged and/or, when R2 represents a hydrogen atom, the
resulting compound is converted into a physiologically tolerable
salt thereof with an inorganic or organic base.
When R" represents a straight chained or branched alkyl
group containing 1 or 2 halo substituents that is/are split off
with the formation of multiple bond(s), the alkyl group may have
one of the meanings given above for straight chained or branched
alkyl groups. As substituents there come into consideration,
fluorine, chlorine, bromine and iodine, fluorine, chlorine and
bromine being especially preferred. As to the number of halogen
substituents, one halogen substituent on the alkyl group
represented by R" is preferred.
The symbol X in the compound of the general formula R"X
may represent the halogen atom fluorine, chlorine, bromine or
iodine, fluorine, bromine or iodine being preferred, an alkane-
sulphonyloxy group with alkane having the meaning methane or
ethane, methane being preferred, or an aryl-sulphonyloxy group
with aryl having the meaning phenyl, for example an ortho-,
meta- or para-alkylphenyl (alkyl = methyl or ethyl), ortho-,
meta- or para-halophenyl or naphthyl group.
~071Z09
. .
; The alkylation in accorda~ce with the procesæ o~ the present
- invention iB carried out in an alkaline medium, pre~erable in
water or in an org~n~c sol~ent, ior example alcohol, dimethyl
sulphoxide, dioxan or tetrahydroiuran, or a mixture thereoi
with water, at a temperature within the range oi ~rom 0 to 150C,
preierably irom 0 to 100C, ior a reaction per~od of 005 hour
to 5 day~ an alka-i~e medium there iæ u~ed, ior e~ample,
1 to 10 equivalents Or an alkall, preierably æodium hydro2ide
or pota~ium hydroxide, calculated on the alkylating agent used,
espe¢ially 2 to 6 equi~alents, in an aqueous alcohol or dimethyl
eulphogide.
The ~-aIkylation may be carried out with an alkylating
agent Or the general ~ormula R"2, in ~hich R~ is pre~ent in the
meaning ri~ally desired ior the symbol ~ in the general iormula
- I, or also - which is ad~antageou~ ior experimental reason~ or
_ may be ne¢e~sary (ior example ~hen ~ represents a vinyl group) -
i oarried out with an alkylating agent Or the general formula R~
in which R" repre~ent~ an alkyl group that i8 ~ubstituted by a
group that can easily be eplit Ori with the iormation oi the
iinally deslred unsQturated carbon-to-carbon bond. Starting
irom such ~ubstituted Rn~-alkylating agent~ the splitting oii
generally take place spontaneously under the alkylating conditions
to iorm the desired end product9 oi the general iormula Io Ii
the ~ubstituents pre~ent in the group xepresented by R" do
not ~plit o~ simultaneoualy under the con~itions Or the
alkylation, the subsequent ~plitting o~f may be carried out in
a known manner with a basic reagent, ior example ~aOH, EOH, E-
,,~
_,~_
. ' :
1071Z09
tertO-butylate, Na~, ~2C03, pyridine or an amine, in a solve~t,
ior example water, alcohol, dimethyl~ormamide or dimethyl
sulphogide, at a temperature within the range of irom O to 150~,
preferably from 20 to 100C, during the cour~e of 0.5 to 10 hours,
preierably 0.5 to 5 hours~
When the primary product of the process of the present
invention does not yet contain the un~aturated carbon-to-carbon
bond in the iinally de~ired position in the group represented
by Rl, it can be shifted by a method generally known to the
expert ior such rearrangement reaction~.
; ~he reaction can be carried out under alkaline or also
acid reaction conditions. ~ basic rearr~ngement catalyst~
; there come into con~ideration, ior example, alk~l~es, ior
example NaOH, EOE or E-tert.-but~late, and as an acid catalyst,
*or example, triiluoracetic acid.
~ ~he baoic rearrangement ii preierableO It i~ carried out,
; ior example, at a temperature within the range oi irom O to 180C,
preierably irom O to ~30C, during the course oi 0.5 to 24 hours,
preierably 2 to 4 hour~, with ~- to 7N-NaOH or EOH in a solvent,
- ior example water, ethylene glycol or dimethyl~ormamide.
~ he -COOR2 group in the 7-position in the product oi the
proces~ oi the present in~ention may be a iree or esteriiied
oarboxyl group. When R2 represents a hydrogen atom in the
#tarting compound, esteriiication oi the iree carboxyl group
may also take place s~multaneously under the aIkylating conditions,
eepecially when R" in the aIkylating agent has the same meaning
a~ ~ .
~, ,, .~,~ /
A -~-
:
- . ~ .
lO~lZO9
When -COOR2 in the starting compound i~ an e~teri~ied
carboxyl group, the ætability oi this ester group depend~ on
the conditions of the alkylating and/or sub~tituent-splitting
oii reaction(s). I* theæe reactionæ are carried out at an
e~pecially high reaction temperature, the ester group i~
wholly or at least partially hydrolysed. Depending on the
iin ~ly deeired mea~ing o~ ~ , by a method known er se a iree
carboxyl group may be esteriiiea or re-esteriiied and an
esteriiied carbogyl group may be hyarolysed.
æalt iormation may be carried out in a manner kno~n ~E ~e,
~or example, with a l-normal æolutions oi the appropriate
inorganic or organic ba~e, and the ~olution so obtained i~
evaporated, and the residue remaining behind i~ recrystallized,
ior example, ~rom an alcohol/water mi~ture (5-9 : 5-1).
~ sually the reaction products are obtained, owing to the
mulbiple bonds in ~ and optionally aleo in R2, in the iorm oi
their cis/trana-isomeric mixtures, whi¢h may be separated in
the U8Ual manner into the cis- and brans-iorms, ior e~ample,
by rraction~l crystA~lization or chromatographyO
~ 8 stated above, the new compo~mds oi the present
invent~on may be admini~tered in the u8ual pharmaceutical iorme
oi application, ior example tablet~, dragée~, capsules, pill~,
solutions~suspensions~ c~.
~ he present invention accordingly ~urther provides a
pharmaceutical preparation whlch compri~es a compol-n~ o~ the
pre~ent invention, in admi~ture or ¢on~u~ction with a pharmaceuti-
cslly ~uitable carrier.
~ 1071Z09
~he new compound~ may be used in combination with the
; additi~es no~mally used in galenical phaxmacy, for e~ample, iormoking preparation~ active against infections o~ the urinary
' tractO
The use oi the new compounds may be carried out by means
oi the u~ual pharmaceutical iorms oi application. ~he compound~
oi the invention are especially suitable ior oral administration;
thus, the pharmaceutical preparations o~ the present i~vention
may be in a iorm suitable iox oral admini~tratio~, ~or e~ample
a~ tablets, dragées, capsules, pills, solutions or ~uspenæion~.
Tablets contain, ior egample, Ool to 1 gram o~ active æub~tance
and 0.1 to 5 grams oi a pharmacologically inert au~iliary
~ub~tance. A~ au~iliary ~ubstances there are used, ior e~ample,
ior tablets, lactoae, starches, talcum, gelatine and magnesium
~teaxate.
The compounds oi the present ln~ention can be used in the
ileld both oi human and veterinary medicine.
`` ~he new aotive substances are used in quantities between
Ool to 4.0 gram~ per patient per dayO
The present i~ve~tion accordingly also provides a method
ior the treatment oi a urinary tract iniection, wherein a
compound oi the present invention i~ a~ministered to a living
beingO ~ "living being" is understood herein to exclude a
human bei4g.
- The iollowing Examp_es ~77ustrate the inYentlon:-
~ 9
.. `.. : . , ` - `. :
.
: 1071209
~xample
5-~llyl-8-oxo-2,3,5,8-tetrahydro fRror2,3-g]quinoline-7-
carbo~ylic acid.
- (a) 5-~llyl-8-o~o-2,3,5,8-tetrahydro-~uror2,3-g]quinoline
7-carbo~ylic acid allyl ester.
.8 Grama oi pulverized soaium hydroxide were ~u~pe~ded
~n 27 ml oi dimethyl ~ulphoxide, then ~ol gram~ oi 8-hydro~y-
2,~-dihydro-~uro~2,~-g]qui~oline-7-carboxylic acid were introduced,
and the whole waæ ætirred ror 10 minutes at room temperature.
; ~hen 5067 ml o~ allyl bromide were added, and the mixture waæ
stirred for 2 hour~ at room temperature, and then poured into
water. ~he solid product wa~ ~iltered oY~ and recr~tallized
~rom ethanolO Melting poi~t: 100C. Yield: 205 gram3.
(b) 5-~llyl-8-oxo-2,3,5,8-tetrahydro-~uror2,3-g]quinoline-
7-carbo~ylic acidO
1.0 Gram oi 5-allyl-8-o~o-2,~95,8-tetrahydro-iuro~2,~-g]
: quinol~ne-7-oarbogylic ac~d allyl ester wa~ boiled under
reilux ior 10 minute~ with 10 ml oi a 2N sodium hydro~ide
colutionO The solution WaB iiltered and acidi~ied. ~he
precipitated product was recr~st~ zed irom dimethylrormamide.
~elting point: 264-279C. Yiela: 0O55 gramO
.:
E~m~e 2
8-Oxo-5-(1-propen~1)-2,3,5,8-tetra ~aro-iuro~2,3-g]
Qul~oline-7-carbo~ylic acid.
.
` ' " "
'`
~ 0 7 1 2 0 9
5Q0 mg oi 5-allyl-8-ogo-2,3,5,8-tetrahydro-furo~2,~-g]
quinoline-7-carboæ~lic acid were heated 1~ 5 ml of a 5N ~odium
hydro~ide solution and 2~5 ml of ethylene glycol ~or 3 hours at
130Co Water and concentrated hydrochloric acid were added,
and the precipitated material wa8 recrystallized ~rom dimethyl-
formamide. Melting point: 263~o Yield: 0030 OEam.
E~am~le ~
~ he sodium ~alt of 8-o~o-5~ propenyl)-2,3,5,8-tetrahydro-
furo~2,3-g]quinoline-7-carbo~ylic acid.
0030 Gram oi 8-ozo-5-(1-propeny1)-2,~,5,8-tetrahydro-
furo~2,3-g]qui~oline-7-carbo~ylic acid wa~ dissolved in lol ml
o~ a lN ~odium hydro~de ~olutio~0 ~he mixture was evaporated
and the residue was recry~tall~zed from isopropanol/water 8:2.
M~lting point: 235Co Yield: 0020 gram.
~X~E~ 4
_, 8-Oæo-2,3,5,8-tetrahydro-5-vinyl-furo[2,3-g]quinoli~e-
7-carboxylic acid.
(a) 6.0 Gram of pul~erized aodium hydroxide were su~pended
in 45 ml of dimethyl sulpho~lde, 5.0 grams of 8-hydro~y-2,3-
dihydro-~urot2,3-g]~uinoline-7-carboxylic acid were introduced,
~nd the whole was otirred for 10 minutes at room temperature.
8.2 gram~ of 1-bromo-2-fluorethane were added, and then the
mi~ture wa~ stirred ~or 2 hours. The mixture Nas poured into
water and acidi~ied with concentrated hydrochloric acid. ~he
resulting solid product ~as chromatographed o~er 200 gram~ of
silica gel with dio~an/conc.~40H/water 8:1:2, and the re~ulting
purified ~ub~tance was recrystallizea irom dimethglformamide.
Melting point: 300_304oc (with decompo~ition)O Yield: 003 gram.
1071Z09
(b) 2.8 Grams of pulverized sodium hydro~ide were suspended
~n 20 ml o~ dimethyl sulpho~ide, 203 gram~ oi 8hydroxy-2,3-
dihydro-~uro~2,3-g]qui~oli~e-7-carboxylic acid were introauced,
and the whole waæ ~tirred ~or 10 minutes at room temperatureO
2.6 ml o~ 1,2-dibromethane were added, and the mi~ture wa~
~tirred ior 24 hours at room temperatureO. ~he miæture was
poured into water ana acidi~ied with concentrated hyarochloric
acidO ~he re~ulting solid product was chromatographed o~er
sil~ca gel with diogan/conc.~H40H/w~ter 8:1:2, and the puriiiea
.~ubstance was recryst~llized ~rom dimethyl~ormam~de.
Melting point: 300-304C (with decomposition)O Yield: 0004 gramO
(c) 2.3 Gram~ oi 8-hydro~y-2,~-dihydro-iuror2,3-g]quinoline-
7-carbo~ylic acid were added to a mixture oi 2.04 grams o~
potassium hydrogide, 14.8 ml o~ water, 46 ml o~ ethanol ana
7.5 ~rams o~ 1,2-bromo~luorethane, and the whole was boiled
under re~lu~ ior 5 daysO he resulting solid product was
iiltered oi~ and chromatographed over 200 grams o~ silica gel
with dioxan/concO~H40~/water 8:1:2, and ~a~ then recrystallized
~rom ethanol. 0.36 Gram oi 5-(2-rluorethyl)-8-oso-2,3,5,8-
tetrahyaro-~uro~2,3-g]quinoli~e-7-carboxylic acid was obtained
with a decompo~ition poi~t o~ 308-314ao 0.36 Gram oi 5-(2-
iluorethyl)-8-oxo-2,3,5,8-tetrahydro-~urot2,3-g]~uinoline-7-
oarboxylic acid was introduced into a mi2ture oi 408 grams of
~odium hydroxide and 3.5 ml of dimethyl ~ulphoxiae, and the
whole wa~ stlrred ior 2 hours at 25C. ~he mixture W28 then
poured into water, and acidiiied with concentrated hydrochloric
--~F --
~071Z09
acid, and the resulting ~olid product was chromatographed over
~ilica gel with dio~an/¢onc.~40~/water 8:1:20 0.20 ~ram oi
8-oxo-2,3,5,8-tetrahydro-5-vinyl-~uro~2,3-g]quinoline-7-carboxylic
acid melting at 300-304C (with decomposition) was obtained.
_ ample_~
8-Oxo-5-propargyl-2,3,5,8-tetrahydro-~uro t 2,3-g]quinoline-
7-carboxylic ac~dO
2.31 Gram~ o~ 8-hydroæy-2,3-dihydro-iuror2,3-g~quinoline-
7-carboxylic acid and 4.7 ml oi 3-bromopropyne ~rere boiled under
reilu~ ior 5 days in a solution o~ 1096 grams oi pota~sium
hydroxide in 1402 ml oi ~rater and 40 ml oi ethanol. he product
that crystalli~ed out aiter cooling ~a~ recr~stallized irom
acetic acid. Melting point: 308-310C. Yield: 107 grams.
amp~,e 6
8-Oxo-5-propadieny1-2,3,5,8-tetrahydro-~uro~2,3-quinoline-
7-carb~xylic aciaO
~ a) 0.54 ~ram oi 8-oxo-5-propargy1-2,3,5,8-tetrahydro-
furo~2,3-g]quinoline-7-carbo~ylic acid wa~ i~troduced into a
~u~pen~ion oi 0056 gram o~ pulverized pota~ium hydroxide a~d
11 ml o~ dimethyl~ormamide at 0C. ~he mi~ture wa~ ~tirred
~or 2 hours at 0C, poured into ~rater, a~d acidiiied with hyaro-
ohloric acid, and the re~ulting solid product was recry~tallized
irom dimethylformamide. Melting point: 257-261Co Yield:
0.16 gram.
'
1 ~ 71 2 0 9
(b) 0~20 Gram o~ 8-oxo-5-propargy1-2,3,5,8-tetrahydro-
~uro~2,~-g]quinoline-7-carbo~ylic acid wa~ stirred in 10 ml
o~ tri~luoracetic acid ~or 20 hours at room temperature, ana
the mi~ture was poured into water, and the re~ulting solid
product ~ra~ recryRtallized ~rom dimethyl~ormamideO Melting
point: 257-261C~ Yield: OolO gramO
am~le 7
5-(2-~utenyl)-8-o~o-2,3,5,8-tetrahydro-~uro~2,3-g~
quinoline-7-car~oæylic acid.
(a) 5-(2-Butenyl)-8-oxo-2,3,5,8-tetrahydro-~uro~2,3-g]
qui~oline-7-carboxylic acid 2-butenyl ester.
2.5 Grams o~ 8-h~droxy-2,~-dihgdro-~uro~2~3-g]~uinoline-
7-carboxylic acid were introduced ~t room temperature into 2
~uepen~ion o~ 300 grams o~ sodium hydro~ide i~ 2108 ml o~
dimethyl sulphoxiae, and then 404 gram~ of crotyl bromiae were
addedO The mixture wa~ stirred ~or 2 hours at room temperature,
and poured into water, and the re~ulting solid product was
re¢rystallized ~rom ethanol. Melting point: ~11C.
Yleld: 0.85 gram.
(b) 5-(2-~utenyl)-8-o~o-2,3,~,8-tetrahydro-~urot2,3-g]
quinoline-7-¢ar~o~ylic acid.
0075 Gram of 5-(2-buten~yl)-8-oxo-2,3,5,8-tetrah~dro-
~uror2,3-g~quinol~ne-7-carbo~licl2-butenyl ester ~a~ bo~led
under reilw~ in 10 ml of a 2N ~odium hydro~ide solutio~ îor 10
minutesO ~he mi~cture wa~ ~iltered, and acidi~ied with hydro-
chloric acld, and the re~ulting solid product wa~ recry~tall~zed
irom dimethylformamideO Melting point: 276-286Co Yield: 0.43
gram.
_ ~ _
~ 0 7 1 Z 0 9
Exam~le 8
5~ uten~ 8-oxo-2,3,5,8-tetrahydro-~uro~2,3-g~
~uinoline-7-carbo~ylic acid.
0.30 Gram o~ 5-(2-~ute~y1)-8-o~o-2,3,5,8-tetrahydro-
~uror2,3-g~quinoline-7-carbo~ylic acid was heatea in 3 ml o~
a 5N ~odium hydroxide solution and 105 ml o~ ethylene gl~col
~or 4 hour~ at 130Co The mi~ture wa~ diluted with some water,
and ac~di~ied with hydrochloric acid, and the re~ulting ~olid
product wae recry~talllzed ~rom dimethyl ~ulphoæide/waterO
Melti~g point: 270-271C. Yield: 0.11 gramO
~amP~e ~
5-(2-Methyl-2-propenyl)-8-oxo-2,3,5,8-tetrahydro-
~uror2,3-g3quinoline-7-carboxylic acidO
(a) ~-(2-Methyl-2-propen~1)-8-oxo-2,3,5,8-tetrahydro-
furo~2,3-g]quinoline-7-carboxylic acid (2-methyl-2-propenyl)
e~ter.
203 Grame o~ 8-hydro~y-2,3-dihydro-~uro~2,3-g]quinoline-
7-carboxylic acid were introduced into a suspensio~ of 3.0
gram~ o~ sod~um hydroxide and 20 ml o~ dimethyl sulpho~ide,
and then 6.0 gram~ of methallyl bromide were added. The m~Yture
wa~ ~tirred for 2 hour~ at room temperature, and poured into
water, and the resulting solid product was recrystallized ~rom
ethanol. Melting poi~t: 169-172Co Yield: 0.80 gramO
(b) 5-(2-Methy1-2-propeny1)-8-oxo-2,3,5,8-tetrahydro-
~Uro~2,~-g3quinoline-7-carbo~ylic acid.
.
. . . - -
,
~071209
0.75 Gram o~ 5-(2-methyl-2-propenyl)-8-ogo-2,3,5,8-
tetrahydro-~uro~2,3-g]quinoline-7-carboxylic acid (2-methy1-2-
propenyl) ester was æuspended in 10 ml of a 2N ~odium hydroæide
solution and boiled under re~lux ~or 10 minu~e~0 ~he mixture
wa~ iiltered, and acidiiied with hydrochloric acid, and the
resulting 801ia product was recrystallized ~rom dimet~yl~ormamideO
Melting point: 265-272Co Yield: 0030 gramO
:3~camPle 10
5-(2-Methyl-l-propenyl)-8-o~o-2,3,5,8-tetrahydro-~uro-
C2.3-g]quinoline-7-carboxylic acid.
0025 Gram oi 5-(2-methyl-2-propenyl)-8-oxo-2,3,5,8-
tetrahydro-iuro~2,3-g]quinoline-7-carbo~ylic acid wa~ heated
in 3 ml o~ a 5N ~oaium hydroxide 301ution and L5 ml 0~ ethylene
glycol for 3 hours at 130C. The mixture was diluted with some
water, and acidi~ied with hydrochloric acid, and the resulting
oolid produc~ ~a~ recry~tallized irom dimeth~liormamide.
Nelting point: 293-295Co Yield: 0.16 gram.
Exam~le 11
~ he ~odium Balt o~ 8-oxo-2,3,5,8-tetrahydro-5-~inyl-~uro
~2, 3-g]quinoline-7-carbo~yll c acid .
O. 23 ~ram o~ 8-oxo-2, 3, 5,8-tetrahydro-5-rinyl- euro t 2, 3-g]
quinoline-7-oarbo~yllc acid wa8 dissolved in 0.9 mi o~ a 1
eodium h;ydroxide solutionO ~he mixture waa concentrated ~,
va,cuo, and the re~idue wa~ recry~tallized ~rom i~opropanol/water
8:20 Melting point: 253-257C. Yield: 0.13 gram.
~ IG