Note : Les descriptions sont présentées dans la langue officielle dans laquelle elles ont été soumises.
11~2 79~
The invention relates to new maytansinoids having anti-
mitotic and/or antitumour activity, and to their preparation.
In a first aspect of the invention, there is provided a
method of producing a maytansinoid of the formula:
CO
CH ~D~ ' 5 1~ G (I)
N ~ o
CH3 CH30 H
A wherein R i9 hydrogen or alkyl of 1 to 15 carbon atoms, which
comprises acylatingf maytansinol with a corresponding carboxylic
acid of the formula:
R-COOH (II)
~!
in the presence of a carbodiimide.
Referring to the abo~e formulae ~I) and (II), the
alkyl of 1 to 15 carbon atoms for R may be straight-chain or
branched alkyl. Examples are: methyl (maytanacine,
Antibiotic C-15003 P-l), ethyl (maytansinol propionate,
Antibiotic C-15003 P-2~, propyl (Ansamitocin P-3',
Antibiot.ic C-15003 P-3'), isopropyl (Ansamitocin P-3, '
Antibiotic C-15003 P-3), n-butyl, isobutyl (Ansamitocin
P-4, Antibiotic C-15003 P-4), secondary butyl, tertiary
butyl, n-pentyl, n-hexyl, n-heptyl, 3-heptyl, n-octyl,
-- 1 --
.~ .
. ~
n-nonyl, n-decyl, n-undecyl, n-dodecyl, n-tridecyl or n-tetradecyl
and n-pentadecyl.
The maytansinoid of the above formula (I) includes novel
compounds, i.e. those having hydrogen, n-butyl or alkyl of 5 to 15
carbon atoms.
Thus, in accordance with a second aspect of the invention
there are provided novel maytansinoids of the formula:
R'
CO
Cl CH O
3 ~N~ CH3 ~Ia)
N ~ O
¦ O H
CH3 3 H
wherein R' is a hydrogen, n-butyl OT alkyl of 5 to 15 carbon
atoms, and a process for producing same by Oee~e~ maytansinol
with a corresponding carboxylic acid of the formula
R'-COOH ~IIa)
.~ .
in the presence of a carbodiimide.
The maytansinoids ~I) and (Ia) are useful as antimicrobial
agents and also as antitumor agents.
The carboxylic acid ~II) or (IIa) may be employed in a
proportion of about 1 to 500, preferably up to about 30, molar
- 2 -
. . .
. .
. ,, , . ,. ~
11~279~3
equivalents, and the carbodiimide in a proportion of about 1 to
700, preferably up to about, 50 molar equivalents, with respect
to the maytansinol.
The carbodiimide may be any compound having a carbodi-
imide linkage ~-N=C=N-) which is convertible to a urea linkage
(-NH-C0-NH-) during the contemplated acylation reaction. Thus,
it may be, for example, a compound of the following formula:
Rl-N=C=N-R (III)
wherein each of Rl and R2 is an organic radical which is capable
of permitting the conversion of the carbodiimide portion to the
corresponding urea during the reaction between maytansinol and
the carboxylic acid ~II). Accordingly, in the generic aspect
of this invention, the actual nature of the substituents Rl and
R is not of primary importance, with the limitation being
present that the substituents permit the conversion of the
carbodiimide group to a urea. Although particularly best results
ha~e been found using dicyclohexylcarbodiimide, Rl and R2 may
also be independently selected from aliphatic and aromatic groups
bearing further substituents that permit the conversion of the
carbodiimide function to the corresponding urea. R and R
may, for example, be independently of each other cycloalkyl
which is unsubstituted or substituted with di-lower alkyl-
amino, lower alkyl which is unsubstituted or substituted
with di-lower alkylamino or morpholino, or phenyl which
is unsubstituted or substituted with lower alkyl.
Some examples of carbodiimides which may be preferably
used are diphenylcarbodiimide, di-o-tolylcarbodi-
' , ~' ' . . . ': ' '- ' ' '
:: ~ . .. ; : - - '
.. ..
,
- . . , . : - :
- -
~ Z~9~3
imide, di-p-tolylcarbodiimide, di-tert.-butylcarbodiimide, etc. as well as
l-cyclo~exyl-3-~2-morpholinoethyl)carbodiimide, 1-cyclohexyl-3-(4-diethylamino-
cyanohexyl)carbodiimide, l-ethyl-3-(2-diethylaminopropyl)carbodiimide, and 1-
ethyl-3-(3-dimethylaminopropyl)-carbodilmide.
This reaction may ~e carried out in a suitable solvent such as esters,
e.g. ethyl acetate; ethers, e.g. diethylether, dioxane, tetrahydrofuran, etc.;
halogenated hydrocarbons, e.g. methylene chloride, chloroform, etc.; nitriles,
e.g. acetonitrile; aromatic hydrocarbons, e.g. benzene; nitromethane; pyridine;
dimet~ylformamide; dimethylsulfoxide; sulfolane; etc.; as well as a suitable
mixture of such solvents.
This reaction may be conducted, for example, at a suitable temperature
from ice-cooling up to refluxing temperature o the reaction system.
The acylation reaction proceeds more smoothly in the presence of a
catalyst which is capable of enhancing the acylation of maytansinol. As examples
of such catalysts there may be mentioned tertiary amines (e.g. aliphatic tertiary
amines such as triethylamine; and aromatic tertiary amines such as pyridine, ~-,
~- or ~-picoline, 2,6-lutidine, 4-dimethylaminopyridine, 4-pyrrolidinopyridine,
dlmethylaniline, diethylaniline, etc.). In many cases, among those catalysts,
4-dimethylaminopyridine is most advantageous. The catalyst is employed in an
2~ amount sufficient to promote the acylation reaction. Thus, such suitable amount
of the catalyst may in many cases be selected from the range of about 0.001 to
10, prefera~ly, from about 0.01 to 1 molar equivalent to the carboxylic acid (II).
~ here such a catalyst is employed, an amount of the carboxylic acid
(I-I~ may generally be reduced preferably to about 1 to about 6 molar equivalents
to maytansinol.
The maytansinoid ~I) produced in the above manner can be isolated and
purified by routine procedures such as concentration, solvent extraction,
-- 4 --
~.
11[3Z798
chromatography, recrystallization, etc.
By the method of this invention thus far described, the maytansinoids
(I) can be synthetically and advantageously produced.
Among maytansinoids (~), those of the formula ~Ia) are novel compounds.
Partîcularly, compounds CIa) having alkyl of 5 to 15 carbon atoms or R' are
characterized by their lessened toxicity. As said alkyls, those of straight
chain are desirable.
The maytansinoids (I) including the compounds ~Ia) obtainable by the
present method have useful antimito~ic activity and antitumour activity. These
activities, coupled with their low toxicity, make the compounds (I) useful for
administration to tumour-bearing warm-blooded animals (e.g. mouseJ rat, rabbit,
dog, cat and human being) by oral and other routes to prolong their life spans.
For the parenteral application of maytansinoid (I), it can be administered by
the subcutaneous, intraperitoneal, intravenous, intramuscular or an other suit-
aale route. The dosage may be chosen from the range of, for example, about 1 to
50 ~g/kg body weight/dose, according to the condition of the disease, animal
~pecies and other factors.
Injectable solutions may be prepared, for example by dissolving about
50 ~g to 3 mg of compound (I) in each about 0.5 mQ of alcohol (e.g. methanol or
2Q ethanoll and adding thereto a sufficient amount of physiological saline to make
10 m~. ~hen the dosage is small, this stock solution may be diluted with an
additional amount of physiological saline.
The maytansinoids (I) according to this invention are useful also in
that they display antimicrobial activities, e.g. antifungal activity and anti-
protozoal actlvity. Thus, in employing compound (I) as an antifugal or anti-
protozoal agent, it is useful for the assay of bacterial flora in samples of
soil, active sludge and animal fluid. Thus, when useful bacteria are to be
.
' '~'' -.
- - - . . .
.
. .
~, . . .. . ~ , .. . . . .
isolated ~ro~ a soil sample or when the action o~ bacteria is to be assayed to
the exclus-ion of the actions of protozoa and fungi for the operation and analysis
of an active sludge system for effluent disposal, the maytansinoid (I) can be
advantageously utilized for the purpose of ensuring a selective growth of bacte-
ria without allowing the concomltant fungi and protozoa to gro~ and multiply.
For such purposes, the test sample is added to a liquid or solid culture medium
and, based on each l mQ of the medium, 0.1 mQ of a solution of about lO to lO0
~g/mQ of compound ~I) in 1% aqueous methanol is added, followed by incubation.
At level of application of 0.02 m~ as a l mg/m~ aqueous solution,
maytansinoid (I) inhibits growth of pathogenic microorganisms such as the caus-
ative agents of the stem rot, Helminthosporium leaf spot and sheath blight of
rice plants. Therefore, for the treatment Gf such plant diseases, the plants
may be sprayed with a solution containing about 0.5 to 5 ~g/mQ of compound ~I)
in 1% aqueous methanol.
Maytanslnol, which is used as the starting compound in the method of
this invention, is known as a principle of plant life [Kupchan et al, J. Amer.
Chem. Soc. 97, 5294 (1975)] and can also be produced by reductive cleavage of
known maytansinoids.
Maytansinol can be advantageously produced also by the following pro-
2~ cedure. Thus, an Antibiotic C-15003 producing microorganism belonging to the
genus Nocardia (Deposit No.; FERM-P No. 3992, IF0-13726, ATCC-31281) is cultivat-
ed in a culture medium to accumulate the ansamitocin of the following formula
(IV) in the culture broth.
-- 6 --
.~ ~
.. .. . .
' '' ' . :
: .
'
Z7~3
R"
C Q ICH3 o o o
3(~ , CH3
CH3 1 ~ IV)
\~l~ N O
CH3 CH30
wherein R" is acetyl, propionyl, iso-butyryl, n-butyryl or iso-valeryl. This
ansamitocin is then reductively hydrolyzed with a metal hydride compound such
as LiAQH4. ~E. Higashide et al, Nature 270, 721 (1977); Offenlegungsschri~t
~West Germany) 27 46 209; and United States Patent 4,137,230].
Incidentally~ the maytansinoids (I~ may be produced also by acylating
maytansinol with use of a reactive derivative with respect to the carboxyl func-
tion of the carboxylic acid (II). As the reactive derivative for this purpose,
there may ~e employed, fQr example, a carboxylic acid derivative having a func-
tlonal group which is able to acylate the 3-position of maytansinol, such as an
acid anhydride or acid halide (e.g. chloride, bromide, etc.) of (II). The solv-
ent and catalyst which can be employed for this purpose are similar to those
mentioned above in connection with the acylation reaction according to the
method of this invention. The reaction temperature may he generally within the
range of a~out 20~C and 40C. In case of an acid anhydride, the acylation may
~e prcmoted by conducting the reaction in the presence of the carbodiimide under
similar conditions to those of the present method.
The method of this invention is superior to the just above-mentioned
acylation reaction employing the reactive derivative of the carbox~lic acid ~II),
~, .. . .
,:
. : : . -.
.
~ Z79~
especially in the yield of the object compound ~I).
The following Examples and Reference Examples are intended merely to
illustrate presently preferred embodiments of the present invention and not to
restrict the scope of this invention.
Throughout the foregoing description as well as in the following
Examples, Reference ~xamples and Claims, "g", "~", "m~", "C" and "N" respec-
tively refer to "gram~s)", "microliter(s)", "milliliter~s)", "degrees centigrade"
and "Normal(s~".
In the following Examples and Reference Examples, silica gel used for
column-chromatography is the commercial product of Kieselgel 60, Art 7734 of
E. Merck, West Germany unless otherwise referred to; melting points were deter-
mined on a Yanagimoto MP-S3 melting point apparatus (Yanagimoto Seisakusho, Kyoto,
~apan), ultraviolet absorption spectra on a Hitachi ESP-3T recording spectro-
photometer, nuclear magnetic resonance spectra on a Varian EM-390 90MHz NMR
spectrometer and mass spectra on a JEOL J~S-OlSC mass-spectrometer.
Example 1
In 10 mQ of methylene chloride were dissolved 100 mg (0.177 m mole)
of maytansinol and 94 mg (1.068 m moles) of isobutyric acid, followed by addition
of 255 mg (1.238 m moles) of dicyclohexylcarbodiimide (hereinafter briefly re-
2~ ferred to as DCC) at about 22 - 25~C. Pollowing the addition of 43 mg (0.352 m
mole~ of 4-dimethylaminopyridine (hereinafter briefly referred to as DMAP), the
mixture was stirred at that temperature for 5 hours, at the end of which time
an addltional 21 mg (0.172 m mole) of DMAP was added. The mixture was stirred
at the same temperature for 18 hours. The resultant precipitate was separated by
filtration and washed with methylene chloride. The filtrate and washings were
combined, washed with water and dried. The solvent was distilled off under re-
duced pressure, then a small amount of ethyl acetate added to the residue and the
'
,~
.
11C~27~38
mixture stirred and filtered to remove the insolubles. The filtrate was
chromatographed on silica gel (75 g) with ethyl acetate saturated with water.
The fractions giving an Rf value of about 0.31 on thin layer chromatography with
the same solvent system were pooled, concentrated under reduced pressure and
allowed to stand, the resultant crystals being collected by filtration. By the
a~ove procedure there was obtained 36.0 mg of compound ~I) (R: isopropyl). The
m.p., NMR, U~, mass spectra and thin layer chromatography data on the above pro-
duct established it to be Ansamitocin P-3.
Example 2
lQ By a procedure similar to that described in Example 1, 100 mg of ma~-
tansinol ~0.177 m mole) was reacted with 103 mg o~ isovaleric acid ~1.010 m moles)
and the crude reaction product was chromatographed. The fractions giving an Rf
value of about 0.35 on thin layer chromatography (solvent: ethyl acetate saturat-
ed with water~ were collected, concentrated under reduced pressure and allowed to
s*and. The resultant crystals were collected hy filtration. By the above pro-
cedure there was obtained 27.5 mg of compound (I) (R: isobutyl). The m.p. NMR
and thin layer chromatography data on this product established it to be Ansamito-
cin P-4.
Example 3
2~ To a solution of maytansinol ~95.8 mg, 0.170 m mole) and n-decanoic
acid (capric acid) (176.8 mg, 1.026 m moles) in dry dichloromethane (5 mQ) was
added DCC (?45 mg, 1.189 m moles) and the mixture was stirred at room temperature
~or a short while until insolubles began to separate out. Then, following addi- -
tion of ~MAP (41.4 mg, 0.339 m mole), the mixture was stirred at that temperature
~or about 4.5 hours, after which a further amount (12.2 mg, 0.1 m mole) of DMAP
was added. The mixture was stirred at room temperature overnight and the in-
solu~les were filtered off. The filtrate was washed wîth 0.5N-HC~ (ca.10 mQ) and
- .-- : '.:
.
. . : . , .... . . . . - . , -, . , -.:. , .
~ -
279~
saturated aqueous sodium hydrogen carbonate (ca. 10 mQ) in that order and dried
over anhydrous sodium sulfate. The solvent was distilled off and the residue
was chromatographed on silica gel (75 g), elution being carried out with ethyl
acetate. The eluate was collected in 16-g fractions, the fractions No. 12
through NG. 40 were pooled and the sol~ent distilled off to recover 56.8 mg of
crude product. This product was rechromatographed on ~ column of silica gel
(25 g) and elution was carried out with chloroform (150 mQ) and chloroform-
methanol (40:1, v/v) (350 mQ) in that order, the eluate being collected in 16-g
fractions. The fractions No. 6 through No. 20 were pooled and the sol~ent was
distilled off to recover a glassy solid. This glassy residue was dissolved in
et~yl acetate and diethyl ether was added. By the above procedure there was
obtained 42.6 mg of maytansinol 3-n-decanoate as a white sandy solid. m.p.
130 - 134C (decomp.)
MM~ spectrum (in CDCQ3) ~ ppm: 0.83(3H, s), 0.85(3H,t,J=5.5Hz),
1.05-1.85(17H, m), 2,2-2.7(4H, m),
2.87(1H, d, J=9Hz), 3.16(3H, s),
3.18(1H, d, J=13Hz), 3.37(3H, s),
3.48(1H, d, J=9Hz), 3.49(1H, d, J=13Hz),
3.97(3H, s), 4.25(1H, m), 4.87~1H, dd, J=
3Hz ~ 12Hz), 5.49(1H, dd, J=9Hz ~ 15Hz),
6.13(1H, d, J=llHz), 6.45(1H, dd, J=llHz
15Hz), 6.64(1H, s), 6.80(1H, d, J=lHz),
6.86(lH, d, J=lHz)
UY spectrum (A max, in MeOH) nm: 233, 240.5, 252.5, 281, 289
MS spectrum ~m/Q): 657, 642, 625, 622, 615, 587
Example 4
The following compounds were obtained by procedures similar to that
_ 10 -
:,
~" ,~,
.
: . . - :
: . . '' .
lll~Z798
descri~ed in Example 3.
In the listing given below, the order of statements is as follows.
The name of the product compound is followed ~y ~l) the amount of maytansinol
used ~mg), (2) the car~oxylic acid used and its amount ~mg~, (3) the amount of
DCC used (mg), (4) the total amount of DMAP used ~mg), ~5) the yield of the pro-
duct compound ~mg), ~6) the melting point of the product compound, ~7) the NMR
spectrum of the product compound ~ ppm, 90 MHz, in CDCQ3), with TMS as internal
reference), (8) the W spectrum of the same compound (~MmaH nm) and (9) the mass
spectrum of the same compound (m/e).
[A] Maytansinol 3-n-heptanoate
1) 102.1
2) Heptanoic acid, 141.4
3) 261.6
4~ 67.3
5) 24.9
6) 158 - 160C (decomp.)
7) 0.84(3H, s), 0.88(3H, t, J~5.5Hz),1.1-1.8~11H, m),
2.14~1H, dd, J=3Hz ~ 14Hz), 2.2-2.54(3H, m), 2.50(1H, dd, J=
12Hz, ~ 14Hz), 2.87(1H, d, J=9Hz), 3.15(3H, s), 3.17(1H, d,
2Q J-13Hz), 3.37(3H, s) J 3.46(1H, d, J=9Hz), 3.49(1H, d, J=
13Hz), 3.99(3H, s), 4.25(1H, m), 4.87(1H, dd, J=3Hz ~ 12Hz),
5.49(1H, dd, J=9Hz ~ 15Hz), 6.13(1H, d, J=llHz), 6.30(1H, s),
6.44(1H, dd, J=llHz ~ 15Hz), 6.80(1H, d, J=lHz), 6.83(1H, d,
J=lHz), etc.
8) 233, 240.5, 252.5, 281, 289
~) 676, 659, 633, 615, 600, 583, 580, 573, 545
C~
.. . . .
': ~ '~ ' ' ' .. ' - ' " ' , ": - -
, . ,- - ~, , ' '
' ' ~ ` ' .- ' .' ,,,~
79~
~B¦ Maytansinol 3-n-tridecanoate
1~ 97.5
2~ Tridecanoic acid, 219.3
3~ 246.3
4) 44.3
5) 39.5
6) 110 - 116C (decomp.~
7) 0.~3(3H~ s), 0.85(3H, t, J=5.5Hz), 1.05-1.85(23~, m~,
2.2-2.7(4H, m), 2.87(lH, d, J=9Hz), 3.16(3H, s),
3.18(1H, d, J=13Hz), 3.37(3H, s), 3.48(1H, d, J=9Hz~,
3.49(lH, d, J=13Hz), 3.97(3H, s), 4.25(lH, m), 4.87(lH,
dd, J~3Hz ~ 12Hz), 5.48~1H, dd, J=9Hz ~ 15Hz), 6.12(1H,
d, J=llHz), 6.44(1H, dd, J=llHz ~ 15Hz), 6.55(1H, s),
6.30~1H, d, J=lHz), 6.86(1HJ d, J=lHz), etc.
8) 233, 240.5, 253, 282, 289.5
9) 699, 684, 667, 664, 657, 629.
Example 5
In 5 mQ of dry dichloromethane was dissolved 101.3 mg of maytansinol,
followed by the addition of n-hexadecanoic acid (palmitic acid) (260.3 mg,
1.015 m moles~, N-cyclohexyl-N'-(3-diethylamino)propylcarbodiimide-hydrochloride
(224.8 mg, 0.911 m mole) and DMAP (43.6 mg, 0.357 m mole). The mixture was stir-
red at room temperature for 3 hours, at the end of which time a further amount
(20.~ mg, a.l71 m mole) of DMAP was added. The mixture was stirred at room
temperature overnight. A still additional amount (44 mg, 0.361 m mole) of DMAP
was further added, followed by stirring at room temperature again overnight.
The reaction mixture was then chromatographed on a column of silica gel ~75 g)
with ethyl acetate, the eluate being collected in 16-g fractions. The fractions
r~
~1
....
- . . . .. . . . .
.
. .
~Z~8
No. 9 through No. 20 were pooled and the solvent was distilled off. The crude
product thus o~tained was rechromatographed under the same conditions as above,
the fractions No. 11 through No. 20 were pooled and the solvent was distilled
off to recover 38.3 mg of residue. This was recrystallized from diethyl ethcr-
hexane to o~tain Z4.0 mg of maytansinol 3-n-hexadecanoate as white powdcrs.
m.p. lOS - 116C ~decomp.~
NMR spectrum (in CDC~3) ~ ppm: 0.83(3H, s), 0.85(3H, t, J=5.5Hz),
1.0-1.85(29H, m~, 1.70(3H, s), 2.2-2.7
(4H, m), 2.87(1H, d, J=9Hz), 3.16(3H, s),
3.18(1H, d, J=13Hz), 3.37(3H, s), 3.47(1H,
d, J=13Hz), 3.97(3H, s), 4.25(1H, m), 4.87(1H,
d, J=3Hz ~ 12Hz), 5.49(1H, d, J=9Hz ~ 15Hz),
6.12(1H, d, J=llHz), 6.45(1H, dd, J=llHz
15Hz), 6.80(1H, d, J=lHz), 6.85(1H, d, J=
lHz), etc.
W spectrum (~MmeH)nm: 233, 240(sh), 252.5, 281, 289.5
MS spectrum (m/e): 741, 726, 706, 699
Example 6
To a solution of maytansinol (107.1 mg, 0.1895 m mole) and DCC
2Q ~195.2 mg, 0.9476 m mole) in 5 mQ of dry dichloromethane, were added 26 ~Q
(0.69 m mole) of 99% formic acid and 46.2 mg (0.3787 m mole~ of DMAP. After
two hours' stirring, DCC (195.6 mg, 0.9495 m mole), formic acid (99%; 26 ~Q,
0.69 m mole) and DMAP (47.0 mg, 0.3852 m mole) ~ere further added and the mix-
ture was stirred overnight at room temperature. The insolubles were filtered
off and the filtrate was evaporated to dryness. The residue was subject to
preparative thin layer chromatography on precoated silica gel plates (Art 5717,
Merc~l developed twice with ethyl acetate saturated wlth water to give 12.2 mg
- 13 -
~.
- - . . .. .. : , - .
'
:
,
~Z798
o~ maytans~inol 3-f~rmate a~ a glassy substance. Rf-value on a thin layer
chromatography~on Merck precoated silica gel plate (HPTLC, Art 5642, E. Merck)
with ethyl acetate saturated with water as solvent: 0.54 (c~., Rf~value of
maytansinol under the same conditions: 0.35~.
Example 7
To a solution of maytansinol (1~3.7 mg, 0.1835 m mole) and n-valeric
acid ~113 mg, 1.108 m moles) in dichloromethane (10 mQ) was added DCC ~265 mg,
1.286 m moles) and the mixture was stirred at room temperature for a short
while. Then, following addition of DMAP (45 mg, 0.369 m mole), the mixture was
stirred at that temperature for 3 hours, after which a further amount (23 mg,
0.18g m mole~ of DM~P was added and the mixture was stirred a~ room temperature
~or 15 hours. The insolubles were filtered off and the filtrate was concentrated
under reduced pressure. The residue was admixed with about 5 mQ of ethyl acetate
to remove the insolubles by filtration. The filtrate was concentrated under
reduced pressure and the residue was purified by three successive chromatography
on silica gel ~1~ 5iO2 (75 g) with ethylacetate, (2) SiO2 (35 g) with chloroform-
methanol (40:1, v/v) and (3) (SiO2 (75 g) ethyl acetate in that order. The frac-
tions giving a spot of Rf value of about 0.34 on thin layer chromatography with
ethyl acetate saturated with water were pooled, concentrated under reduced pres-
sure. To the residue dissolved in ethyl acetate was added diethyl ether to give
precipitates which were harvested by filtration. By the above procedure there
was o~tained 35 mg of maytansinol 3-n-pentanoate as crystals.
m.p. 165 - 168RC
NM~ s~ectrum (i~n CDCQ3) ~ ppm: 0.83(3H, s, 4-CH3), 0.96(3H, t~ J=6Hz;
-COCH2CH2CH2CH3~, 4.87~lH, dd, J=3Hz ~ 12Hz;
3-H~, 6.83(2H, su~s~antially singlet; aromatic H)
~: ,
, -
. : . :
:
.
~27~8
Example 8
In a manner similar to those o~ the preceding ExamplesJ there are ob-
ained the ~ollowing compounds:
~C] Maytans~nol_3-n-~exanoate
m.p. 159 - 162~C (decomp.)
NMR spectrum (in CDCQ3) ~ ppm: 0.83(3H, s), 0~87(3H, t, J=6Hz), 1.05-1.8
(9H, m), 1.6g(3H, s), 2.2-2.54(4H, m),
2.87(1H, d, J=9Hz), 3.16~3H, s), 3.22(1H, d,
J=13Hz), 3.37(3H, s), 3.46(1H, d, J=9Hz),
3.49~1H, d, J-13Hz), 3.97(3H, s), 4.21(lH, m),
4.87~1H, dd, J=3Hz ~ 12Hz), 5.49~1H, dd, J=
9Hz ~ 15Hz), 6.12~1H, d, J=llHz), 6.44~1H, dd,
J=llHz ~ 15Hz), 6.79(1H, d, J=lHz), 6.81~1H,
s), 6.82(1H, d, JslHz), etc.
UV spectrum (in MeOH, A max) nm: 233.5, 241, 252.5, 281, 289
MS spectrum ~m/e): 615, 601, 586, 569, 566, 559, 531
~D] Maytansinol 3-n-octanoate
m.p. 151 - 160C ~decomp.)
MM~ spectrum ~in CDCQ3) ~ ppm: 0.83~3H, s), 0.87~3H, t, J=5.5Hz), 1.1-1.8
~13H, m), 1.68~3H, s), 2.2-2.7~4H, m),
2.86~1H, d, 3=9Hz), 3.15~3H, s), 3.19~1H,
d, J=13Hz), 3.37~3H, s), 3.40~1H, ~road),
3.48~1H, d, J=9Hz), 3.50~1H, d, J=13Hz),
3.97(3H, s), 4.24(1H, m), 4.85~1H, dd,
J=3Hz ~ 12Hz), 5.48(1H, dd, J=9Hz ~ 15Hz),
6.11(1H, d, J=llHz), 6.43~1H, dd, J=llHz
15Hz), 6.77~1H, d, J=lHz), 6.80(1H, s),
6.83(1H, d, J=lHz).
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C,i
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:
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ll~Z7~3
UV spectrum ~in MeQH, A max) nm: 233.S, 241, 252.5, 281, 289
MS spectrum (m/e): 62~, 614, 597, 594, 587, 55
~eference Example 1
To a mixed solution of 108.1 mg maytansinol and 302 mg isobutyric an-
hydride in 5 mQ of dichloromethane was added 47.1 mg of DMAP at room temperature.
The mixture was stirred at that temperature for 100 minutes, at the end of which
time a further 24.2 mg of DMAP was added. The entire mixture was stirred at
room temperature overnight. Next morning, the reaction mixture was shaken with
10 mQ of 0.5N-HC~ and the dichloromethane layer was taken and dried ~over
Na2S04). The solvent was distilled off and the residue was chromatographed on
silica gel (75 g) (solvent: ethyl acetate: ethy~l acetate saturated with water
= 2 : l~v/v).) The eluate was collected in 16-g fractions and the fractions
No. 17 through No. 30 were pooled and concentrated under reduced pressure. The
residue was dissolved in 5 mQ of chloroform, washed with 10% aqueous sodium
hydrogen carbonate and water in that order and dried ~over Na2S04~. The solvent
was distilled off under reduced pressure, the residue dissolved in a small amount
o~ acetic acid, allowed to stand and the resultant crystals recovered by filtra-
tion. By the above procedure there was obtained 35.5 mg of compound ~ R:
lsopropyl] m.p. 190 - 192C. The elemental analy~sis, mass spectrum, IR spectrum,
20. W spectrum and thin layer chromatogram (Rf) of this product were in agreement
with those o~ Ansamitocin P-3.
Reference Example 2
In a mixture of 1 mQ triethylamine and 50 mQ of dimethylformamide was
d~ssol~ed 100 mg of maytansinol and, while the solution was stirred under ice-
cooling, 50 ~Q of isobutyryl chloride was added dropwise. The mixture was
stirred under ice-cooling for 30 minutes and then, at room temperature for 2.5
hours, after which it was distilled under reduced pressure. The residue was
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ll~Z798
dissolved in 1~ m~ of dichloromethane, washed with water and dried ~Na2504).
The solvent was distilled o~f under reduced pressure and the residue was
chromatographed and recrystallized as in Reference Example 1. By the above pro-
cedure there was obtained 19.4 mg of Ansamitocin P-3.
Reference Exam~le 3
In 1.0 mQ of dichloromethane was dissolved 23.5 mg of maytansinol and,
at 22C, 70.5 mg (ca. 10 mol.equiv.) o~ acetic-formic anhydride (prepared by
cooling 2 mQ of acetic anhydride to -5C - 0C, adding 1 mQ of 99~ formic acid
at that temperature over about 10 minutes, heating the mixture to SO~C for 15
minutes and cooling it rapidly to 0C), as well as 11.7 mg of DMAP, was added.
The mixture was stirred at room temperature (ca. 22C.~ overnight. Then, 10
drops of methanol ~ere added to this reaction mixture, followed by stirring at
room temperature for 3 hours. The mixture was concentrated to dryness under
reduced pressure, the residue was applied to a sillca gel preparative thin layer
chromatoplate, and was developed twice with water-saturated ethyl acetate. The
zone near 6.0 to 8.0 cm above the origin was scraped o*fthe plate and extracted
wlth 10% methanol-dichloro methane. The solvent was then distilled off under
reduced pressure. By the above procedure there was obtained 8.35 mg of may-
tans~inol 3-formate as a colorless glassy substance.
2~ NMR spectrum ~in CDCQ3) ~ ppm: among others, 0.85 ~3H, s; 4-CH3), 4.83~1H,
dd, J=3Hz ~ llHz; 3-H), 8.01(1H, s; 3-OCHO)
Re~erence Example 4
To a solution of maytansinol ~102.7 mg) in dichloromethane (10 m~)
there were added caproic anhydride (145.7 mg), DCC (207 mg) and DMAP (46.5 mg),
and the mixture was stirred at room temperature for 5 hours. The reaction mix-
ture was chromatographed on silica gel with ethyl acetate to obtain 56.5 mg of
may*ansinol 3-n-octanoate
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