Note : Les descriptions sont présentées dans la langue officielle dans laquelle elles ont été soumises.
P-2707 21 ~L 6 0 6 1 P~TENT
Unit Test, Kit, and Method for Qualit~tive Identificatio
of a SusPeCt Substance
Field of the Invention
The present invention relates to qualitative identification of a suspect substance
and more particularly to a kit with self-contained unit-~ests for visualization of the
presence of controlled substances such as cocaine, heroin, and tetrahydrocannabinol
(cannabis).
DescriptionofRelatedInforrnation
Generally speaking, tests for qualitative identification of a suspect substance
consist of a reagent or reagents, a sampling apparatus and a vessel for rnixing the
reagent with the sample and observing the result. There have been efforts to make the
tests easier to use by p?~çl~in~ the reagents in unit dose ampules, self measuring
sampling devices and combinations of sampling devices and test vessels. The use of
ampules for reagents improved the ease and safety of handling the identificationreagent, some of which are toxic and corrosive. These sampler, reagent, vessel
devices are 5~ti~f~ctory for identific~tions of suspect substances, but have proved to be
time con.c~-min~, are often clifficult to use in the field and are not practical for broad or .:
covert screening of luggage pieces, clothing and the like for trace amounts of suspect
substances such as heroin, cocaine and cannabis derivatives.
Currently, in such screening situations, investigators use dogs which have been ~ :
trained to react to the odor of the suspect substances. Additionally, there are sprays
available which induce a color development when sprayed on a specific substance.The use of spray reagents which leave a residue is generally precluded in screening
situations by liability considerations. Further, compounds present in some qualitative
identification reagents are toxic and corrosive, obviously limiting the ability of
investigators to use them in screening situations. .
The task of investig~tors screening for suspect substances would be made .
easier if a self contained unit test was available which could be easily and rapidly used,
which did not leave residue behind. Additionally, if such tests were provided in a kit . .
which included one or more qualitative identification unit tests for several substance as
well as a recovery system for acquisition of evidentiary samples, the task of
investigators would be further f~çilit~te~ Such unit tests and kits, as well as methods
for their use, are described herein below.
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' P-2707 2 ~ 6 1
Summarv of the Invention
A self-contained unit test of the present invention for qualitative identification
of a suspect substance includes a vi~u~li7~tion reagent solution having a compound
5 which undergoes a visible color change when contacted by a suspect substance. The
unit test further includes a pad formed from a bibulous material impregnated with the ~ ::
reagent solution and a unit package for co~ fillg the pad and ",~i"~ g the reagent :
solution in an as-filled, ready-to-use state during storage.
A self-contained unit test of the present invention for qualitative identification
of cocaine includes a visualization reagent prepared as an as~ueous solution of i
cobaltous thiocyanate, glycerin, tartaric acid and boric acid, and a bibulous pad :
impregnated with the reagent solution. The pad colllai~ g the solution is sealed in a
unit package to maintain the reagent on the pad in an as-filled, ready-to-use state .
15 during storage.
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A self-contalned umt test of the pr~sent Inventlon for qualltatlve Identlficatlon . . .
of heroin includes a visualization reagent prepared as a methanolic solution of ~ ~ :
cobaltous thiocyanate, ammonium metavanidate and yellow dye, and a bibulous pad : ~
impregnated with the reagent solution. The pad containing the solution is sealed in a : . :
unit package to maintain the reagent on the pad in an as-filled, ready-to-use state :
during storage. ~ -
A self-contained unit test of the present invention for qualitative identification
of tetrahydrocannabinol (THC) includes a vi.cu~li7~tic~n reagent prepared as an aqueous
ethanolic solution of ortho-dianis;(lil~e, and a bibulous pad impregnated with the -'
reagent solution. The pad conk.illillg the solution is sealed in a unit package to :~
maintain the reagent on the pad in an as-filled, ready-to-use state during storage. ;
A kit for the qualitative idçntific~tion of suspect substances of the present
invention includes at least one self-contained unit test for qualitative identification of a
suspect substance col~ lising a vi-~u~1i7:-tion reagent including a solution which
undergoes a visible color change when contacted with a suspect substance, a bibulous
pad impregnated with the visualization reagent, and a unit package for containing the
pad. The package serves to maintain the reagent on the pad in an as-filled, ready-to-
use state during storage. The kit further includes instructions for using the unit test
and an over package for colllaining the unit test or tests and the instructions.
A method for visualization of a suspect substance on a surface of a sample .
includes providing ai unit test inchl(ling a visualization reagent for the suspect
substance, a pad formed from a bibulous material having a surface and impregnated - : :
with the vi~ li7~tion reagent. The pad is additionally sealed in a unit package for
mRintzlining the reagent in an as-filled, ready-to-use condition. The pad is removed
from the package and wiped on the surface of the sample having the suspect substance. . -~
The pad surface is then observed for any color change, ti-e color change being
inclicative of contact with the suspect substance.
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P-2707
Brief Description of Drawin~s :
Fig. 1 is a perspective view of a pad of the present invention showing the unit package
S in phantom;
Fig. 2 is a sketch showing the usage of the pad of the present invention to identify a
suspect substance on a surface; ! .
10 Fig. 3 is a sketch showing the surface of the pad of the present invention folded upon
itself after contacting a surface which may have thereon particles of a suspect
substance; and
Fig. 4 is a sketch showing the surface of the pad of the present invention after15 contacting a surface having thereon particles of a suspect substance.
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P-2707
2~16~6:~ :
Detailed Description of the Preferred Embodiments
While this invention is satisfied by embodiments in many different forrns, thereis shown in the drawings and will herein be described in detail, preferred embodiments
of the invention with the understanding that the present disclosure is to be considered
exemplary of the invention and is not intended to limit the invention to the
embodiments illustrated. The scope of the invention will be measured by the appended
claims and their equivalents.
,
A self contained unit test of the present invention for qualitative idPntific~tion
of a suspect substance is illustrated in ~igs. 1-4 and is generally dçsi~n~ted as 20.
Referring to Fig. 1, the unit test 20 includes a vis~ i7~tion reagent 22 which
undergoes a visible color change when contacted by a suspect substance. The
visualization reagent 22 is impregnated onto a pad 24 forrned from a bibulous material
which serves to hold reagent 22 and deliver it for contacting a suspect substance. ~ ~ .
Pad 24 impregnated with reagent 22 is contained in a unit package 26 which
serves to maintain reagent 22 in an as-filled, ready-to-use state during storage.
A first preferred embodiment of unit test 20 is as a qualitative identification test
for cocaine, here considered either as the free base, Cl 7H2 lNO4, as a salt of the base,
or when mixed with common diluents.
The visualization reagent for cocaine includes preferably about 0.1 to about 10
percent, most preferably about 0.86 percent cobaltous thiocyanate; preferably about ~:
0.1 to about 10 percent, most preferably about 0.86 percent, tartaric acid; preferably
al~out 0.1 to about 10 percent, most preferably about 0.86 percent boric acid; ~ :
preferably about 30 to about 65 percent, most preferably about 54.3 percent glycerin;
and water, preferably in a quantity sufficient to make 100 percent, most preferably
43.1 percent. The ingredients are placed in a clean vessel at ambient temperature and : :
stirred until solid components are substantially dissolved. .
Pad 24 is formed from a bibulous material which preferably is a fibrous
nonwoven, such as cotton, rayon, polyester, blends of cotton, rayon, polyester, and
most preferably is formed from nonwoven rayon with a basis weight of 160 grams. - ~
Preferably about 0.5 to 1.5 ml, and most preferably about 0.8 ml of the cocaine '
visualization reagent is applied to pad 2'1 which preferably is sized to fit in the hand of
the user, most preferably a'oout 2.5 x 3.4cm. Pad 24 impregnated with the y~rell~d
cocaine visualization reagent is preferably sealed in an appropriately labeled unit
package which is formed from materials which serve to maintain reagent 22 in an as-
filled, ready-to-use state during storage. An example of the first preferred
visualization reagent 22, when the suspect substance is cocaine, is given as Example 1.
' P-2707
Example 1
Preparation of a unit-test for qualitative
S idPntific~tion of cocaine.
I. Cocaine Vi~ li7~tion Rea~ent ~ - -In a clean, well-stirred mixing vessel place the ~ .
following components (all values in parts per hundred; ~.
w./wt.):
1. 43.1 l-water (meeting USP criteria for . ~ .
purified water), :
2. 0.86-cobaltous thiocyanate (Sigma C-
5637 or eg.);
stir for about 1 hour at ambient temperature to
e~ect dissolution, then add;
3. 54.31-glycerin (USP grade);
4. 0.86-tartaric acid (~JSP grade);
5. 0.86-boric acid (USP grade); and .
continue stirring at ambient temperature to a ' ~.
substantially uniform solution.
II. Pad Preparation
To a nonwoven rayon pad (e.g. Champion Edison
160 gram nonwoven rayon, M.P.D. 24-001) 2.54cm x
3 .4cm, add 0.8 rnl of the above cocaine visualization .
reagent.
.
III. Packa~e Pr~alalion
Each individual pad i.l.pre~"lated with 0.8 rnl of : :
the above cocaine vi~u~li7~tion reagent is sealed in a '
package formed from an upper web and a lower web
both composed of ethylene-acrylic acid
copolymer/~ n;n-lm foil/polyethylene/paper to forrn a .
package resistant to moisture and air permeation which is
capable of ..,~ g the unit-test in an as-lSlled, ready-
to-use state during storage.
40 ~ i; IV. Function
An unused pad impregnated with the above
cocaine ,ri.~ 1i7~tion reagent has a light pink color.
When the swface of the pad contacts cocaine, the area of
contact develops a blue color, similar to Pantone Process .
Blue, indicating the presence of cocaine.
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P-2707 S~116~
A second preferred embodiment of unit test 20 is as a qualitative identificationtest for heroin, here considered either as the -free base C21H23NOs as a salt ofthe
base or when mixed with common diluents.
The vi.cu~li7~ion reagent for heroin includes preferably a methanolic solution
about 0.1 to about 10 percent, most preferably about 1.25 percent of cobaltous
thiocyanate; preferably about 0.05 to about 5.0 percent, most preferably about 0.28
percent, metavanidate; about 0.01 to about 1 percent, most preferably about 0.63percent, aqueous yellow dye and methyl alcohol, preferably in a quantity sufficient to
make 100 percent, most preferably 97.86 percent. The cobaltous thiocyanate and the
ammonium
metavanidate are both prepared as stock solutions, then filtered, with the filtrates being
mixed with the yellow dye to complete the preparation of the heroin vi.~ li7.~tion
reagent.
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Preferably about 0.1 to about 1.5 ml, and most preferably about 0.8ml, ofthe
heroin vi.~ 1i7:lfion reagent is applied to pad 24 which is sized to fit in the hand of the
user, most preferably about 2.5 by 3.4 cm. Pad 24 i~ aled with the preferred
heroin vi.cu~ ion reagent is preferably sealed in an al~ploplia~ely labeled unitpackage which is formed from materials which serve to maintain reagent 22 in an as
filled ready-to-use state during storage. An example of the second preferred ~ :
vis~l~li7~tion reagent 22, when the suspect substance is heroin is given as Example 2.
Example 2
Preparation of a unit test for qualitative identification of heroin.
I. Heroin VisualizationRea~ent - : ~
Prepare the following stock solutions: ~ I
A.) Stir 2.0 gm cobaltous thioeyanate (Sigma C-5637 or eq.) into 48 ~:
gm methanol (Reagent Grade) for S min. at ambient temperature; filter
(Whatman #l or eq.); filtrate is solution A;
B.) Stir O.5gm ammonium metavanidate (Aldrich 20,555-9 or eq.) into
160 gm methanol ~eagent Grade), filter (Whatman #1 or eq.); filtrate is
solution B; and
C.) Solution C is ac~ueous yellow dye (McCormick UPC#07092 or eq.).
In a clean well stirred vessel, mix at ambient temperature, 24 gm of
solution A with 56 gm of solution B and I gm of solution C; stir to a
substantially uniform solution to form the heroin visualization reagent. :
II. Pad preparation
To a nonwoven rayon pad (e.g. Champion Edison 160 gm nonwoven
rayon, M.P.D. 24-001) 2.5 by 3.4 cm add 0.8 ml of the above heroin
visualization reagent.
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~2707
21~60~ -
III. Packa~e Preparation
Each individual pad impregnated with 0.8 ml of the above heroin
visualization is sealed in a package formed from an upper web and a lower web
S both composed of ethylene-aclylic acid copolymer/aluminum foil/
polyethylene/paper. This forms a package, resistant to moisture and air
permeation, which is capable of maintaining the unit test in an as-filled, ready-
to-use state during storage.
IV. Function
An unused pad impregnated with the above heroin visualization reagent
has a pale yellow color. When the surface of the pad contacts heroin, the area
of contact develops a light green color, similar to Pantone 340C, indicating the -:
presence of heroin.
A third preferred embodiment of unit test 20 is as a qualitative ide~ltir1c~tiontest for delta 9-tetrahydrocannabinol, C21H30o2~ including but not limited to
marijuana, hashish and hash oil. For the purposes of this document, all
tetrahydrocamlabinol co.liai.ling substances will hereinafter be referred to as "THC".
. .
The vi.~ li7~tion reagent for THC includes preferably about 0.09 to about 10
percent, most preferably about 0.35 percent ortho~ niqi~line; and preferably sufficient
aqueous 60 percent ethyl alcohol to make 100 percent, most preferably 99.65 percent. .~::
The ortho-dianisidine preferably is well mixed with the aqueous ethanol, then filtered,
the filtrate being the reagent.
Preferably about 0.5 to 1.5, ml and most preferably about 0.8 ml, of the heroin : ~
vi.~ li7~tion reagent is applied to pad 24 which is sized to fit in the hand of the user, : :
most preferably about 2.5 by 3.4 cm. Pad 24 i...ple~,..a~ed with the preferred THC
vi~ i7~tion reagent is preferably sealed in an app~op~ia~ely labeled unit package -~
which is formed from materials which serve to maintain reagent 22 in an as-filled
ready-to-use state during storage. An example of the third preferred visualization
reagent 22 when the suspect substance is THC is given as ~xample 3.
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~; 35 Example 3
;: : P~epaldtionofaunittestforqualitativeidentificationoftetrahydrocannabinol
(THC).
I. THC vi~ li7~tion rea~ent
Stir 0.3 gm of ortho-dianisidine (Sigma D-3502 or eq.) into 85 gm of '
60 percent aqueous ethanol (45 gm Baker 5-9401 or eq. and 40 gm Water
[meeting USP criteria ~or purified water] at ambient conditions; filter
(Whatrnan #1 or eq.); filtrate is the THC reagent.
II. Pad pre~dif~lion
To a nonwoven rayon pad (e.g. Champion Edison 160 gm nonwoven
rayon, M.P.D. 24-001) 2.5 by 3.4 cm add 0.8 ml of the above THC vi~ li7~t;on
reagent.
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-~-2707 2~0~
III. Packa~e Preparation
Each individual pad impregna~ed with 0.8 ml of the above THC
vis11~1i7~tion reagent is sealed in a package formed from an upper web and a
lower web both composed of ethylene-acrylic acid copolymer/aluminum
foil/polyethylene/paper. This forms a package, resistant to moisture and air :
permeation, which is cap~ble of m~int:linin~ the unit test in an as-filled, ready-
to-use state during storage.
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l O IV. Function
An unused pad impregnated with the above THC vi~ li7.;1tion reagent
has an off-white color. When the surface of the pad contacts a material
col"~illillg THC, the area of contact develops a red-brown color, similar to
Pantone 201C, in one to two minutes at ambient te~lpe~ e indicating the
presence of THC. Color development and iderltific~ion maybe f~cilit~ted by
folding the contact surface back onto itself, thereby assuring that both sides of
any suspect particulate comes in contact with the reagent.
In cases where a positive id~ntific~ n for a suspect substance is made using : .
one of the preferred self contained unit tests described herein above, rules of evidence
require that additional samples be acquired in an unaltered state for col~ atory assa~
by alternate analytical methods using reference standard compounds. To assist
investigators in acquiring these evidentiary samples, a kit may include instructions, one ~ -
or more of one or all of the self contained unit tests for vi~ i7~ti~n of suspect
s~slallces. The kit may also include a pad, as shown in Figs. 1 and 2 as pad 24,ill,ple~,llated with 70 percent aqueous isopropyl alcohol. This pad may be used for
wiping a surface or an article which qualitatively test positively for a suspect substance
to pick up the s~bst~nçe from the surface without ch~n~in~ it. The pad with the
unchanged suspect substance may then be placed in a clean inert plastic bag and
sealed. The kit may include such bags for colll~ g and prOte.;th~g the pad with the
sample, as well as appl Opl iaie labels. Preparation of a sample collection pad is
described as Example 4. :
Example 4
Non-DestructiYe Sample Collection Pad
I. Collection Rea~eent
70 percent isopropyl alcohol (USP or eq.)
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~ 2707 21~061
II. Pad preparation
To a nonwoven rayon pad (e.g. Champion Edison 160 gm nonwoven
rayon, M.P.D. 24-001) 2.5 by 3.4 cm, add 0.8 ml of the above collection
S reagent.
III. Packa~e Preparation
Each individual pad impregnated with 0.8 ml of the above collection ;
reagent is sealed in a package formed from an upper web and a lower web both
composed of ethylene-acrylic acid copolymer/aluminum foil/
polyethylene/paper. This forms a package, resistant to moisture~ and air
permeation, which is capable of mfffint~fining the collection reagent pad in an as- ~ ~ -
filled, ready-to-use state during storage.
IV. Function -
An unused pad ill~yl~ated with collection reagent is used to wipe a
surface containing substances for confirr ffatory analysis. The pad containing the
substances is then placed in an inert evidence collection bag, sealed, and labeled.
A method for using unit test 20 for vi~l-71i7ing a suspect substance is shown inFig. 2. Unit test 20 is provided, then pad 24 containing reagent 22 is removed from
unit package 26. The to be tested sample 28 contains a suspect material 30 on its
surface 32. Pad 24 has an upper surface 34 and a lower surface 36. For the purposes
of conducting the test, either surface is identical. The operator wipes surface 32, -: . .. ~ .:
25 co~f~laii)~i~g suspect ~ff~ff;~ff~fallce 30 thereon, with surface 36 of pad 24. Both pad 24 and -
surface 32 may be at ambie~nt lelllpel~ res. On pad surface 36 which contacts sample '
surface 32, a color change 38 will occur in any area which contacted suspect substance
30. -
In the first preferred embodiment, where visualization reagent 22 is a
qualitative identification for cocaine, unused pad surface 36 has a light pink color,.
Color change 38 would develop as a blue color in the case where suspect subs~ance 30 ~ :
was cocaine. -
In the second preferreffi embodiment, where visualization reagent 22 is a
qualitative identiffcation for heroin, unused pad surface 36 has a pale yellow color. '
Color change 38 would develop as a light green color in the case where the suspect .. ~
~: s~f~nce was heroin. . --
In the third preferred embodiment, where visualization reagent 22 is a fqualitative identification for 'FH'C, unused pad surface 36 has an off-white color. : ~ .
~Color change 38 would develop a~er one to two minutes as a red-brown color where
the suspect s~lbst~.nce is THC. :
In all cases, but particularly for the THC reagent, the development of the colormay be fz.cilitsffted by folding the wiping surface over on itself, as shown in l~ig 3.
This folding ff~nhsffnf ~ff~S contact of the vie~ i7~tion reagent with both sides of any ' . ~ ~ :
particles picked up during wiping, thereby ~nh~n~ing the sensitivity of the test to the
presence of any suspect substance.
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-2707 21 16 ~ 6 1
One skilled in the art of qualitative identification of suspect substances will
recognize that the size and composition of the pad, the amount of the visualization
reagent and the composition of the visualization reagent itself may be varied toS accommodate particular applications and particular suspect substances. Kits may be
prepared with multiple tests for multiple substances in a pack including instructions for
use, color comparison charts, non-destructive evidence collection pads, inert sealable
evidence collection bags, labels and the like as well as disposable gloves. The use of
disposable gloves would help to ensure that any positive test result obtained was due
10 only to materials present on the surface of the article being tested and further, that any
sample collected using the sample collection pad was uncontaminated.
Thus, it can be seen that the present invention provides the art and practitioners
of qualitative identification of suspect substances with a simple, easily used unit test, :
15 kit and method suitable for screening, qualitative identification and e~ridence collection.
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