Note : Les descriptions sont présentées dans la langue officielle dans laquelle elles ont été soumises.
CA 02301201 2000-02-04
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METHOD OF INHIBITING CATHEPSIN K
Field of the Invention
5 This invention relates to a method of inhibiting cathepsin K by the
utilization
of the physical and spacial characteristics that allow for the interaction of
the
propeptide with the active site of the enzyme. This interaction inhibits the
activity
of cathepsin K and this information is useful in the design of specific
chemical
compounds for treating diseases in which said inhibition is indicated, such as
10 osteoporosis and periodontal disease. This invention also relates to a
novel
crystalline structure of procathepsin K, the identification of the propeptide
bound to
the catalytic active site for this enzyme and methods enabling the design and
selection of inhibitors of said active site.
15 Backgyround of the Invention
Cathepsin K is a member of the family of enzymes which are part of the
papain superfamily of cysteine proteases. Cathepsins B, H, L, N and S have
been
described in the literature. Recently, cathepsin K polypeptide and the cDNA
encoding such polypeptide were disclosed in U.S. Patent No. S,S01,969 (called
20 cathepsin O therein). Cathepsin K has been recently expressed, purified,
and
characterized. Bossard, M. J., et al., (1996) J. Biol. Chem. 271, 12517-12524;
Drake, F.H., et al., (1996) J. Biol. Chem. 271, 12511-12516; Bromme, D., et
al.,
( 1996) J. Biol. Chem. 271, 2126-2132.
Cathepsin K has been variously denoted as cathepsin O, cathepsin X or
25 cathepsin 02 in the literature. The designation cathepsin K is considered
to be the
more appropriate one (name assigned by Nomenclature Committee of the
International Union of Biochemistry and Molecular Biology).
Cathepsins of the papain superfamily of cysteine proteases function in the
normal physiological process of protein degradation in animals, including
humans,
30 e.g., in the degradation of connective tissue. However, elevated levels of
these
enzymes in the body can result in pathological conditions leading to disease.
Thus,
cathepsins have been implicated in various disease states, including but not
limited
to, infections by pneumocystis carinii, trypsanoma cruzi, trypsanoma brucei
brucei,
and Crithidia fusiculata; as well as in schistosomiasis malaria, tumor
metastasis,
35 metachromatic leukodystrophy, muscular dystrophy, amytrophy, and the like.
See
International Publication Number WO 94/04172, published on March 3, 1994, and
references cited therein. See also European Patent Application EP 0 603 873
Al,
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WO 00/09653 PCT/US98/14634
and references cited therein. Two bacterial cysteine proteases from P.
gingivallis, -
called gingipains, have been implicated in the pathogenesis of gingivitis.
Potempa,
J., et al. ( 1994) Perspectives in Drug Discovery and Design, 2, 445-458.
Cathepsin K is believed to play a causative role in diseases of excessive bone
5 or cartilage loss. Bone is composed of a protein matrix in which spindle- or
plate-
shaped crystals of hydroxyapatite are incorporated. Type I Collagen represents
the
major structural protein of hone comprising approximately 90% of the
structural
protein. The remaining 10% of matrix is composed of a number of non-
collagenous
proteins, including osteocalcin, proteoglycans, osteopontin, osteonectin,
10 thrombospondin, fibronectin, and bone sialoprotein. Skeletal bone undergoes
remodeling at discrete foci throughout life. These foci, or remodeling units,
undergo
a cycle consisting of a bone resorption phase followed by a phase of bone
replacement.
Bane resorption is carried out by osteoclasts, which are multinuclear cells of
15 hematopoietic lineage. The osteoclasts adhere to the bone surface and form
a tight
sealing zone, followed by extensive membrane ruffling on their apical (i.e.,
resorting) surface. This creates an enclosed extracellular compartment on the
bone
surface that is acidified by proton pumps in the ruffled membrane, and into
which
the osteoclast secretes proteolytic enzymes. The low pH of the compartment
20 dissolves hydroxyapatite crystals at the bone surface, while the
proteolytic enzymes
digest the protein matrix. In this way, a resorption lacuna, or pit, is
formed. At the
end of this phase of the cycle, osteoblasts lay down a new protein matrix that
is
subsequently mineralized. In several disease states, such as osteoporosis and
Paget's
disease, the normal balance between bone resorption and formation is
disrupted, and
25 there is a net loss of bone at each cycle. Ultimately, this leads to
weakening of the
bone and may result in increased fracture risk with minimal trauma.
The abundant selective expression of cathepsin K in osteoclasts strongly
suggests that this enzyme is essential for bone resorption. Thus, selective
inhibition
of cathepsin K may provide an effective treatment for diseases of excessive
bone
30 loss, including, but not limited to, osteoporosis, gingival diseases such
as gingivitis
and periodontitis, Paget's disease, hypercalcemia of malignancy, and metabolic
bone
disease. Cathepsin K levels have also been demonstrated to be elevated in
chondroclasts of osteoarthritic synovium. Thus, selective inhibition of
cathepsin K
may also be useful for treating diseases of excessive cartilage or matrix
degradation,
35 including, but not limited to, osteoarthritis and rheumatoid arthritis.
Metastatic
neoplastic cells also typically express high levels of proteolytic enzymes
that
2
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degrade the surrounding matrix. Thus, selective inhibition of cathepsin K may
also
be useful for treating certain neoplastic diseases.
Surprisingly, the propeptide of cathepsin K exhibits characteristics that
allow
for the interaction of the propeptide with specific residues of the active
site of
5 cathepsin K and this information is useful in the design of specific
chemical
compounds as inhibitors of cathepsin K and would be useful for treating
diseases in
which inhibition of bone resorption is indicated, such as osteoporosis and
periodontal disease. Thus, this invention relates to the method of inhibiting
cathepsin K using the crystal structure of procathepsin K hereinbelow defined.
Summary of the Invention
In one aspect, the present invention provides a method for designing
compounds to inhibit cathepsin K with certain structural, physical and spacial
characteristics that allow for the interaction of said compounds with specific
residues
15 of the active site of the enzyme in a manner similar to the interaction of
the
propeptide with the enzyme active site. This interaction inhibits the activity
of
cathepsin K and, thus, treats diseases in which bone resorption is a factor.
In another aspect, the present invention provides a novel cysteine protease
proenzyme in crystalline form, in particular this invention relates to a novel
procathepsin K crystal.
In yet another aspect, the invention provides a novel protease proenzyme
composition, specifically a novel procathepsin K composition characterized by
a
three dimensional catalytic site inhibited by the atoms of the propeptide
residues
listed in Table IV.
25 In still another aspect, the invention provides a method for identifying
inhibitors said method comprising the steps of: providing the coordinates of
the
proenzyme structure of the invention to a computerized modeling system; and
identifying compounds which will hind to the structure in a manner similar to
that of
the propeptide.
Other aspects and advantages of the present invention are described further in
the following detailed description of the preferred embodiments thereof.
Brief Description of the Drawings
Figure 1 is the amino acid sequence of procathepsin K aligned with the
amino acid sequences of other cysteine protease proenzymes.
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Figure 2 is a ribbon diagram of procathepsin K. The amino and carboxyl-
terrnini are indicated by N and C. The drawing was produced using the program
MOLSCRIPT [Kraulis, P., J. Appl. Crystallogr., 24, 946-950 ( 1991 )].
Figure 3 is an illustration of the active site of procathepsin K.
Table I provides the three dimensional protein coordinates of the
procathepsin K crystalline structure of the invention.
Table II provides a listing of the three atom angles between atoms of the
propeptide and the protein for all propeptide atoms within 5 t~ngstroms of the
protein.
10 Table III provides a listing of the distances between atoms of the
propeptide
and the protein for all propeptide atoms within 5 angstroms of the protein.
Table IV provides the atoms of the amino acid residues of the catalytic site
and the propeptide amino acid residues which interact with them.
Detailed Description of the Invention
The present invention provides a novel cysteine protease proenzyme
crystalline structure, and methods of use of the crystalline form and active
site
interactions with the inhibitory propeptide to identify protease inhibitor
compounds.
In particular, the present invention provides a method for inhibiting
cathepsin
K by designing compounds with certain structural, physical and spatial
characteristics that allow for the interaction of said compounds with specific
residues
of the active site of the enzyme based on the structure of the proenzyme. This
interaction inhibits the activity of cathepsin K and, thus, treats diseases in
which
bone resorption is a factor.
25 In another aspect, the present invention provides a novel cysteine protease
proenzyme in crystalline form, in particular procathepsin K in crystalline
form as
defined by the positions in Table I herein.
In still another aspect, the present invention provides a novel protease
proenzyme composition, specifically a novel procathepsin K composition,
3o characterized by a three dimensional catalytic site formed by the atoms of
the amino
acid residues listed in Table IV herein.
A key aspect of this invention provides a method of inhibiting cathepsin K
which comprises administering to a mammal in need thereof a compound that
mimics the interaction of the propeptide with the residues of the active site
of
35 procathepsin K. Specifially, this compound comprises:
4
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(i) an oxygen atom which interacts with a hydrogen atom donated by the
indoie nitrogen of tryptophan 283 wherein the distance between these two atoms
is
2.7-3.5~;
(ii) a hydrophobic group that interacts with tryptophan 283 wherein the
distance between the centroid of said hydrophobic group and the centroid of
the side
chain atoms of tryptophan 283 is 4.10-7.10A;
(iii) a hydrophobic group that interacts with tyrosine 166 , methionine
167, alanine 233, leucine 259, and leucine 308, creating a hydrophobic pocket,
and
has distance ranges between the centroid of said hydrophobic group and the
1 o centroids of the side chain atoms of the amino acid residues of said
hydrophobic
pocket which are tyrosine 166: 4.91- 5.91, methionine 167: 5.74-6.74t~,
alanine
233: 4.15-5.15, leucine 259: 6.18-7.18t~, and ieucine 308: 5.71-6.71; and
(iv) an amino group with a pKa of less than 7 or an oxygen atom, each of
which interacts with a hydrogen atom donated by the amide nitrogen of glycine
264
wherein the distance between these two atoms is 2.7-3.SA. In particular, the
preferred compound consists of a hydrophobic group that interacts with said
hydrophobic pocket wherein said hydrophobic group is an isobutyl group.
The method of the instant invention comprises compounds characterized by
the interactions of the atoms of the procathepsin K active site and the atoms
of the
propeptide in the region of the acive site according to Table IV.
This invention also includes a composition comprising procathepsin K in
crystalline form wherein the procathepsin K in crystalline form is
characterized by
structure coordinates according to Table I. In particular, the composition has
an
active site cavity formed by the amino acids in Table IV wherein said active
site is
characterized by the distance between the atoms of Table II and the bond
angles
between interresidue atoms of Table III.
In another aspect of this invention is an isolated, properly folded
procathepsin K molecule or fragment thereof having a conformation comprising a
catalytically active site formed by the residues listed in Table IV, said
active site
defined by the protein coordinates of Table I and a peptide, peptidomimetic or
synthetic molecule which binds with the active site cavity of procathepsin K.
Importantly, this invention provides for a method of identifying an inhibitor
compound capable of binding to, and inhibiting the proteolytic activity of,
cathepsin
K, said method comprising:
introducing into a suitable computer program information defining an
active site conformation of a procathepsin K molecule comprising a
catalytically
active site formed by the residues listed in Table IV, said active site
defined by the
5
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WO 00/09653 PCT/US98/14634
protein coordinates of Table I, wherein said program displays the three-
dimensional
structure thereof;
creating a three dimensional representation of the active site cavityin
said computer program;
displaying and superimposing the model of said test compound on the
model of said active site;
assessing whether said test compound model fits spatially into the
active site;
preparing said test compound that fits spatially into the active site;
using said test compound in a biological assay for a protease
characterized by said active site; and
determining whether said test compound inhibits cathepsin K activity
in said assay.
This invention involves a method of drug design comprising using the
~ 5 structural coordinates of a procathepsin K crystal to computationally
evaluate a
chemical entity for associating with the active site of cathepsin K.
Alternately, this invention provides for a method of identifying inhibitors of
cathepsin K said method comprising the steps of:
providing the coordinates of the atoms of the procathepsin K active
site and the coordinates of the atoms of the propeptide in the region of the
active site
according to Table IV to a computerized modeling system, wherein said system
displays the three-dimensional structure thereof;
displaying and superimposing a model of a test compound on the
model of the propeptide;
assessing whether said test compound interacts with the active site of
procathepsin K in a manner similar to that of the propeptide;
preparing said test compound that interacts with the active site of
procathepsin K in a manner similar to that of the propeptide; and
testing said test compound for cathepsin K inhibitory activity.
30 The three dimensional (3D) structure of the instant protease proenzyme
reveals that human cathepsin K is highly homologous to other known cysteine
proteinases of the papain family in the region of the mature enzyme. Cathepsin
K is
synthesized and secreted from the cell as an inactive proenzyme.
Autoproteolytic
degradation of the N-terminal 99 amino acid pro-peptide produces the active,
mature
35 form of cathepsin K. It is presumed that the activation of procathepsin K
in vivo
occurs in the bone resorption pit which has a low pH environment. The instant
invention provides for the crystal structure of human procathepsin K at 2.6
6
CA 02301201 2000-02-04
WO 00109653 PCT/US98/I4C34
~ngstroms resolution. Knowing the structure of procathepsin K provides a basis
for
the design of inhibitors of cathepsin K.
Listed in Figure 1 are the known amino acid sequences for the proenzymes of
the papain superfamily of cysteine proteases: cathepsin K, cathepsin S,
cathepsin L,
actinidin, cathepsin H and cathepsin B, aligned to illustrate the homologies
there
between. According to the present invention the crystal structure of human
procathepsin K has been determined at 2.6 angstroms. The structure was
determined using the method of molecular replacement and refined to an Rc
values
of 0.204. Further refinement of the atomic coordinates will change the numbers
in
10 Table I. Refinement of the crystal structure from another crystal form will
result in a
new set of coordinates, determination of the crystal structure of another
cysteine
protease will also result in different set of numbers for coordinates in Table
I which
has an experimental error of approximately 0.4 angstroms. Also, for example,
the
amino acid sequence of the cysteine proteases can be varied by mutation
derivatization or by use of a different source of the protein.
Human procathepsin K contains 314 amino acids and the model of the
enzyme provided herein is represented by residues S to 314. The first five
amino
acid residues are not well ordered in the structure.
The procathepsin K crystal structure reveals a conformation of the propeptide
bound to the active site that is heretofor unknown and comprises a distinct
three
dimensional arrangement of atoms.
Table I discloses the protein coordinates of procathepsin K. These data are
reported for the crystal structure described herein. The data are reported in
0
Angstroms with reference to an orthogonal coordinate system in standard
format,
illustrating the atom, i.e., nitrogen, oxygen, carbon, sulfur (at oc,13, y, 8,
or E,
positions in the amino acid residues); the amino acid residue in which the
atom is
located with amino acid number, and the coordinates X, Y and Z in Angstroms
(E1)
from the crystal structure. Note that each atom in the active site and the
entire
structure has an unique position in the crystal. The data also report the B or
30 Temperature Factor values, which indicate the degree of thermal motion of
the atom
in root mean square displacement measurements (~2). Figure 1 illustrates the
procathepsin K crystalline structure of the invention, including the active
site.
The active site of procathepsin K and the propeptide is shown in Figure 2.
The pro-peptide of procathepsin K exhibits a globular domain formed by three
35 alpha-helices and one beta-strand which is located above the right domain
of
cathepsin K. An extended loop stretches from the globular domain through the
active site and connects at the bottom of the mature cathepsin K. The RMS
7
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deviation between the CA atoms of the mature cathepsin K and the CA atoms of
the
refined proenzyme structure is only 0.46 ~ (215 CA pairs).
Residues 74 to 80 of the pro-peptide lie in the active site and are shown in
Figure 2. The residues from cathepsin K which form contacts with the
propeptide
5 are shown as a ball-and-stick model. The orientation of the propeptide in
the active
site cleft is opposite to that postulated for a natural substrate. The S'
subsites
(located above Cys 124 in Figure 2) are occupied by Met 75 (S2') and Thr 76 (S
1').
The S subsites are occupied by Gly 77 (S1), Leu 78 (S2) and Lys 79 (S3). The
main
chain atoms of the propeptide form numerous hydrogen bonds to mature cathepsin
K
10 and are listed as follows: Lys 74 to Cys 121; Met 75 to Gln 118 and Trp
283; Gly
77 to Gly 165, and Lys 79 to Gly 165. The sidechains of the propeptide form
extensive contacts with mature cathepsin K. The side chain of Met 75 is
located in a
hydrophobic area surrounded by side chains of Leu 63 and Met 66 from the
propeptide and Trp 283 from mature cathepsin K. Thr 76 forms hydrogen bonds
15 from its sidechain hydroxyl group to mainchain carbonyls from residue Val
72 and
Asn 260. Additionally, the side-chain methyl group of Thr 76 faces the
hydrophobic
cluster formed by residues Trp 283 and His 261. Gly 77 is located at the
center of
the active site within van der Waals radius of the catalytic cysteine. A
residue with a
larger sidechain would not fit at this position. Leu 78 occupies the S2 active
site
2o hydrophobic pocket, which is formed by Tyr 166, Met 167, Ala 233, Ala 262
and
Leu 308. Lys 79 faces away from the catalytic site and forms a salt bridge
with Asp
160.
The propeptide is anchored to the mature protein by hydrophobic interactions
and hydrogen bonding. The hydrophobic face of helices 2 and 3, and a beta
strand
25 pack against the apolar surface of mature cathepsin K (Figure 3). The beta
strand
from the propeptide hydrogen bonds with a betastrand from mature cathepsin K
forming an anti-parallel beta sheet. The following residues from helices 2 and
3 and
the beta-strand; Tyr 41, Iie 42, His 45, Ala 49, His 54, Tyr 56, Leu 58, Met
60, His
62, Leu 63, Met 66, Val 71, Val 72 and Met 75, form hydrophobic contacts with
side
3o chain atoms od mature cathepsin K. The apolar surface of the mature enzyme
is
formed by residues Ser 240, Phe 241, Phe 243, Tyr 244, Tyr 249, Tyr 250, His
261,
Trp 283, Trp 287 and Met 295. Six of these sidechains, Phe 241, Tyr 244, Tyr
249,
His 261, Trp 283, Trp 287 form an aromatic-aromatic stacking network. Near the
active site a second region of apolar interactions exists. Residues Leu 78 and
Val
35 80 pack against residues Tyr 166, Met 167, Ala 233 and Leu 308. A third
region of
hydrophobic interactions occurs near the C-terminus of the propeptide with
residue
8
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WO 00/09653 PCT/US98/14634
His 84, Leu 91, Tyr 92 and Ile 93 packed against residue Tyr 209, Ile 212, Pro
231
and Phe 311 (Figure 3).
Electrostatic interactions in the form of salt bridges are likely to play a
significant role in the stabilization of the globular structure in the N-
terminal domain
of the propeptide. There are seven salt bridges within the pro-peptide. These
are
Asp 8 to Arg 32, Lys 20 to Asp 65, Asn 24 to Asp 27, Lys 25 to Glu 28, Arg 31
to
Glu 70, Arg 32 to Glu 36 and Glu 48 to His 54. Asp 27 and Asp65 also form
important hydrogen bonds to Asn 23 and Trp 14, respectively. Activation of the
proenzyme occurs at low pH. Therefore disruption of these salt bridges at low
pH
10 rnay disrupt the globular structure and lead to auto-activation of the
proenzyme.
The crystal structure of procathepsin K of the present invention reveals the
three dimensional structure of novel active. site interactions between the
atoms of the
amino acid residues of the mature and propeptide listed in Table IV.
This structure is clearly useful in the structure-based design of protease
15 inhibitors, which may be used as therapeutic agents against diseases in
which
inhibition of bone resorption is indicated. The discovery of the procathepsin
K
catalytic site permits the design of potent, highly selective protease
inhibitors.
Another aspect of this invention involves a method for identifying inhibitors
of procathepsin K characterized by the crystal structure and novel active site
20 described herein. The novel protease crystal structure of the invention
permits the
identification of inhibitors of protease activity. Such inhibitors may bind to
all or a
portion of the active site of cathepsin K; or even be competitive or non-
competitive
inhibitors. Once identified and screened for biological activity, these
inhibitors may
be used therapeutically or prophylactically to block protease activity.
25 One design approach is to probe the cathepsin K of the invention with
molecules composed of a variety of different chemical entities to determine
optimal
sites for interaction between candidate cathepsin K inhibitors and the enzyme.
For
example, high resolution X-ray diffraction data collected from crystals
saturated
with solvent allows the determination of where each type of solvent molecule
sticks.
30 Small molecules that bind tightly to those sites can then be designed and
synthesized and tested for their cathepsin K inhibitor activity.
This invention also enables the development of compounds that can
isomerize to short-lived reaction intermediates in the chemical reaction of a
substrate
or other compound that binds to or with cathepsin K. Thus, the time-dependent
35 analysis of structural changes in cathepsin K during its interaction with
other
molecules is permitted. The reaction intermediates of cathepsin K can also be
deduced from the reaction product in co-complex with cathepsin K. Such
9
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information is useful to design improved analogues of known cysteine protease
inhibitors or to design novel classes of inhibitors based on the reaction
intermediates
of the cathepsin K enzyme and cathepsin K inhibitor co-complex. This provides
a
novel route for designing cathepsin K inhibitors with both high specificity
and
stability.
Because cathepsin K may crystallize in more than one crystal form, the
structure coordinates of procathepsin K, or portions thereof, as provided by
this
invention are particularly useful to solve the structure of those other
crystal forms of
cathepsin K. They may also be used to solve the structure of cathepsin K
mutants,
10 cathepsin K co-complexes, or of the crystalline form of any other protein
with
significant amino acid sequence homology to any functional domain of cathepsin
K.
One method that may be employed for this purpose is molecular
replacement. In this method, the unknown crystal structure, whether it is
another
crystal form of cathepsin K, a cathepsin K mutant, or a cathepsin K co-
complex, or
the crystal of some other protein with significant amino acid sequence
homology to
any functional domain of cathepsin K, may be determined using the procathepsin
K
structure coordinates of this invention as provided in Table I. This method
will
provide an accurate structural form for the unknown crystal more quickly and
efficiently than attempting to determine such information ab initio.
20 Thus, the procathepsin K structure provided herein permits the screening of
known molecules and/or the designing of new molecules which bind to the
protease
structure, particularly at the active site, via the use of computerized
evaluation
systems. For example, computer modelling systems are available in which the
sequence of the protease, and the protease structure {i.e., atomic coordinates
of
25 procathepsin K and/or the atomic coordinate of the active site cavity, bond
angles,
dihedral angles, distances between atoms in the active site region, etc. as
provided
by Table I may be input. Thus, a machine readable medium may be encoded with
data representing the coordinates of Table I in this process. The computer
then
generates structural details of the site into which a test compound should
bind,
3o thereby enabling the determination of the complementary structural details
of said
test compound.
More particularly, the design of compounds that bind to or inhibit cathepsin
K according to this invention generally involves consideration of two factors.
First,
the compound must be capable of physically and structurally associating with
35 cathepsin K. Non-covalent molecular interactions important in the
association of
cathepsin K with its substrate include hydrogen bonding, van der Waals and
hydrophobic interactions.
CA 02301201 2000-02-04
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Second, the compound must be able to assume a conformation that allows it
to associate with cathepsin K. Although certain portions of the compound will
not
directly participate in this association with cathepsin K, those portions may
still
influence the overall conformation of the molecule. This, in turn, may have a
5 significant impact on potency. Such conformational requirements include the
overall three-dimensional structure and orientation of the chemical entity or
compound in relation to all or a portion of the binding site, e.g., active
site or
accessory binding site of cathepsin K, or the spacing between functional
groups of a
compound comprising several chemical entities that directly interact with
cathepsin
1o K.
The potential inhibitory or binding effect of a chemical compound with
cathepsin K may be estimated prior to its actual synthesis and testing by the
use of
computer modelling techniques. If the theoretical structure of the given
compound
suggests insufficient interaction and association between it and cathepsin K,
15 synthesis and testing of the compound is obviated. However, if computer
modelling
indicates a strong interaction, the molecule may then be synthesized and
tested for
its ability to bind to cathepsin K in a suitable assay. In this manner,
synthesis of
inoperative compounds may be avoided.
An inhibitory or other binding compound of cathepsin K may be
2o computationally evaluated and designed by means of a series of steps in
which
chemical entities or fragments are screened and selected for their ability to
associate
with the individual binding pockets or other areas of cathepsin K.
One skilled in the art may use one of several methods to screen chemical
entities or fragments for their ability to associate with cathepsin K and more
25 particularly with the individual binding pockets of the cathepsin K active
site or
accessory binding site. This process may begin by visual inspection of, for
example,
the active site on the computer screen based on the cathepsin K coordinates in
Table
I. Selected fragments or chemical entities may then be position cathepsin K.
Docking may be accomplished using software such as Quanta and Sybyl, followed
30 by energy minimization and molecular dynamics with standard molecular
mechanics
forcefields, such as CHARMM and AMBER.
Specialized computer programs may also assist in the process of selecting
fragments or chemical entities. These include:
~ GRID [P. J. Goodford, "A Computational Procedure for Determining
35 Energetically Favorable Binding Sites on Biologically Important
Macromolecules",
J. Med. Chem., 28:849-857 (1985)]. GRID is available from Oxford University,
Oxford, UK.
11
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~ MCSS [A. Miranker and M. Karplus, "Functionality Maps of Binding
Sites: A Multiple Copy Simultaneous Search Method", Proteins: Structure.
Function
and Genetics, 11:29-34 (1991)]. MCSS is available from Molecular Simulations,
Burlington, MA.
5 ~ AUTODOCK [D. S. Goodsell and A. J. Olsen, "Automated Docking
of Substrates to Proteins by Simulated Annealing", Proteins: Structure.
Function.
and Genetics, 8:195-202 ( 1990)]. AUTODOCK is available from Scripps Research
Institute, La Jolla, CA.
~ DOCK [I. D. Kuntz et al, "A Geometric Approach to Macromolecule-
Ligand Interactions", J. Mol. Biol., 161:269-288 (1982)]. DOCK is available
from
University of California, San Francisco, CA.
Additional commercially available computer databases for small molecular
compounds includes Cambridge Strucutral Database and Fine Chemical Database,
for a review see Rusinko, A., Chem. Des. Auto. News 8, 44-47 (1993).
15 Once suitable chemical entities or fragments have been selected, they can
be
assembled into a single compound or inhibitor. Assembly may be proceeded by
visual inspection of the relationship of the fragments to each other on the
three-
dimensional image displayed on a computer screen in relation to the structure
coordinates of cathepsin K. This would be followed by manual model building
20 using software such as Quanta or Sybyl.
Useful programs to aid one of skill in the art in connecting the individual
chemical entities or fragments include:
CAVEAT [P. A. Bartlett et aI, "CAVEAT: A Program to Facilitate
the Structure-Derived Design of Biologically Active Molecules", in Molecular
25 Reco ni~n Chemical and Biological Problems", Speical Pub., Royal Chem. Soc.
78, pp. 182-196 (1989)]. CAVEAT is available from the University of
California,
Berkeley, CA.
~ 3D Database systems such as MACCS-3D (MDL Information
Systems, San Leandro, CA). This area is reviewed in Y. C. Martin, "3D Database
3o Searching in Drug Design", J. Med. Chem., 35:2145-2154 ( 1992).
~ HOOK (available from Molecular Simulations, Burlington, MA).
Instead of proceeding to build a cathepsin K inhibitor in a step-wise fashion
one fragment or chemical entity at a time as described above, inhibitory or
other type
of binding compounds may be designed as a whole or "de novo" using either an
35 empty active site or optionally including some portions) of a known
inhibitor(s).
These methods include:
12
CA 02301201 2000-02-04
WO 00/09653 PCTIUS98/14634
~ LUDI [H.-J. Bohm, "The Computer Program LUDI: A New Method
for the De Novo Design of Enzyme Inhibitors", J. Comp. Aid. Molec. Des'l~n,
6:61-
78 (1992)]. LUDI is available from Biosym Technologies, San Diego, CA.
~ LEGEND [Y. Nishibata and A. Itai, Tetrahedron, 47:8985 (1991)].
LEGEND is available from Molecular Simulations, Burlington, MA.
~ LeapFrog (available from Tripos Associates, St. Louis, MO).
Other molecular modelling techniques may also be employed in accordance
with this invention. See, e.g., N. C. Cohen et al, "Molecular Modeling
Software and
Methods for Medicinal Chemistry", J. Med. Chem., 33:883-894 ( 1990). See also,
10 M. A. Navia and M. A. Murcko, "The Use of Structural Information in Drug
Design", Current Opinions in Structural Bioloay, 2:202-210 ( 1992). For
example,
where the structures of test compounds are known, a model of the test compound
may be superimposed over the model of the structure of the invention. Numerous
methods and techniques are known in the art for performing this step, any of
which
15 may be used. See, e.g., P.S. Farmer, Drug Design, Ariens, E.J., ed., Vol.
10, pp 119-
143 (Academic Press, New York, 1980); U.S. Patent No. 5,331,573; U.S. Patent
No.
5,500,807; C. Verlinde, Structure, 2:577-587 ( 1994); and I. D. Kuntz,
Science,
257:1078-1082 (1992). The model building techniques and computer evaluation
systems described herein are not a limitation on the present invention.
20 Thus, using these computer evaluation systems, a large number of
compounds may be quickly and easily examined and expensive and lengthy
biochemical testing avoided. Moreover, the need for actual synthesis of many
compounds is effectively eliminated.
Once identified by the modelling techniques, the protease inhibitor may be
25 tested for bioactivity using standard techniques. For example, structure of
the
invention may be used in binding assays using conventional formats to screen
inhibitors. Suitable assays for use herein include, but are not limited to,
the enzyme-
linked immunosorbent assay (ELISA), or a fluoresence quench assay. See, for
example, the cathepsin K activity assay of Example 2 below. Other assay
formats
30 may be used; these assay formats are nvt a limitation on the present
invention.
In another aspect, the protease structure of the invention permit the design
and identification of synthetic compounds and/or other molecules which have a
shape complimentary to the conformation of the protease active site of the
invention.
Using known computer systems, the coordinates of the protease structure of the
35 invention may be provided in machine readable form, the test compounds
designed
and/or screened and their conformations superimposed on the structure of the
13
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protease of the invention. Subsequently, suitable candidates identified as
above may
be screened for the desired protease inhibitory bioactivity, stability, and
the like.
Once identified and screened for biological activity, these inhibitors may be
used therapeutically or prophylactically to block cathepsin K activity.
5 The following examples illustrate various aspects of this invention. These
examples do not limit the scope of this invention which is defined by the
appended
claims.
EXAMPLE 1: Analysis of the Structure of procathepsin K
A. Expression, Purification and Crystallization
Plasmid constructions. Procathepsin K {procat K) was expressed
using the PET system (Novagen). In order to express procat K, an existing
clone
containing the full-length human preprocathepsinK cDNA was subject to PCR
15 amplifcation using oligos designed to introduce a Met residue at amino acid
18 of
the full-length preproenzyme. The resulting fragment was cleaved with NcoI and
BamHI and this fragment was inserted into the pETl6b vector, resulting in
pETprocat K.
Expression of recombinant, human procathensin K. Isolation and
2o solubilization of inclusion bodies. Recombinant pET vectors were
transformed into
strain BL21DE3 (Novagen). Bacteria were grown at 37C to OD600=0.6-0.7 and
induced with 1mM IPTG for 2 hours. The cell pellet from 6 L of bacterial
culture
weighing 15 g was washed with 150 mL of 50 mM Tris/HCl, 5 mM EDTA, 150 mM
NaCI, pH 8Ø After centrifugation at 13,000 x g for 15 rains, the washed
pellet was
25 resuspended into 150 mL of the same buffer prepared at 4~ C and lysed by
passage
twice through a cell disruptor (Avestin) at 10,000 psi. The lysate was
centrifuged as
above, the supernatant decanted and the pellet suspended in 150 mL 50 mM
Tris/HCI, 6 M guanidine HCI, 10 mM DTT, 5 mM EDTA, 150 mM NaCI, pH 8Ø
After stirring for 1 hr at room temperature, insoluble cellular debris was
removed by
3o centrifugation at 23,000 x g for 30 rains and the supernatant clarified by
filtration
(0.45 um PVDF, Millipore). The resulting solution was assayed for protein
concentration according to the method of Bradford using the BioRad protein
asay
and bovine serum albumin obtained from Pierce as standard.
Refolding and Purification. 645 mg of procathepsin K was refolded
35 by quick dilution of the denatured protein solution into 10 L of stirring,
N2 (g)
spraged 50 mM Tris/HCI, 5 mM EDTA, 10 mM reduced and 1 mM oxidized
glutathione, 0.7 M L-arginine pH 8.0 prepared at room temperature. The
solution
14
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was stirred overnight at 4° C then concentrated using tangential flow
followed by a
stirred cell (Amicon), both fitted with lOK membranes. The concentrate was
clarified by centrifugation and filtration then dialyzed against 25 mM Na2P04,
1.0
M NaCI, pH 7.0 (buffer A). The 44 mL dialysate containing 343 mg of protein
was
applied at a LFR= 23 cmlhr in two runs to a 2.6 x 90 cm column of Superdex 75
(Pharmacia) pre-equilibrated in buffer A. Fractions containing procathepsin K
were
identified and pooled based upon purity as observed on 15% acylamide SDS-PAGE
gels prepared according to the method of Laemmli (Laemmli U.K., {1970) Nature
227, 690-685). Rainbow high molecular weight markers (Amersham) were used as
standards. Gels were visualized with 0.25% Brilliant Blue R stain (Sigma). A
portion of the 259 mg of protein recovered from the size exclusion columns was
concentrated to 20 mg/mL using a centricon-10 then diluted 1:1 with 25 mM
Na2P04 pH 7.0 to yield a 10 mglmL solution in 25 mM , 0.5 M Na.CI, pH 7Ø
Crystals of procathepsin K grew to a size of approximately 0.3 mm3 in ten
days at 4° C. The concentration of procathepsin K used in the
crystallization was
l0mglml. The hanging drops vapour diffusion method was used with a solution of
8-10% PEG6000, O.1M Na citrate at pH 5Ø X-ray diffraction data were measured
from a single crystal using a Siemens two-dimensional position-sensitive
detector.
Crystals of the complex are orthorhombic, space group C2221, with cell
constants of
a=56.81 ~, b=156.81 ~, and c=96.10 t~. The crystals contain one molecule in
the
asymmetric unit and contain 60% solvent.
B. Model Building and Refinement
The structure of procathepsin K was solved by molecular
replacement using the program XPLOR (Brunger, A. T., Kuriyan, J. and Karplus,
M.
(1987) Science 235, 458-460). The coordinates for 217 residues from the
structure
of human cathespin K (Zhao, B. et al. ( 1977) Nature Struct. Biol. 4, 109-111
) were
used as a starting model. The cross rotation function was calculated using
data from
10-3.5 t~ with a radius of integration of 23 ~ in a P 1 cell for the search
model with
dimensions of a=88.0 ~, b=74.0 ~, c=68.0 t~. The search was carried out with
pseudo-orthogonal euler angles in Patterson space from 81 0-180°,
82 0-90, and 83 0-720°. The highest peak was 15.7 6. The translation
search was
computed using data from 10 -3.5 ~ and gave a peak at 26 6. The resulting
model
yielded a R-factor of 0.43. The R-factor for the model dropped to 0.40 after
rigid
body refinement from 10-3.5 ~ and to 0.31 after 200 cycles of positional
refinement.
The electron density maps were improved using solvent flattening and by
histogram matching as implemented in the module DM of the CCP4 suite of
CA 02301201 2000-02-04
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programs (Collaborative Computational Project, Number 4 (1994), "The CCP4
Suite: Programs for Protein Crystallography", Acta Cryst. D50, 760-763).
The modified electron density map was to build the model of used
procathepsin K. Conventional positional refinement was used to refine the
structure
during model building. The structure was refined using X-plor. The final model
of
procathepsin K consists of residues 5 to 214. The simulated annealing
refinement
was finally carried out to a final R of 0.204. The backbone conformation of
all
residues is within the Ramachandran plot limits.
1 o EXAMPLE 2: Assavs
Determination of cathepsin K proteolytic catalytic activity
All assays for cathepsin K were carried out with human recombinant
enzyme. Standard assay conditions for the determination of kinetic constants
used a
fluorogenic peptide substrate, typically Cbz-Phe-Arg-AMC, and were determined
in
100 mM Na acetate at pH 5.5 containing 20 mM cysteine and 5 mM EDTA. Stock
substrate solutions were prepared at concentrations of 10 or 20 mM in DMSO
with
uM final substrate concentration in the assays. All assays contained 10% DMSO.
Independent experiments found that this level of DMSO had no effect on enzyme
20 activity or kinetic constants. All assays were conducted at ambient
temperature.
Product fluorescence (excitation at 360 nM; emission at 460 nM) was monitored
with a Perceptive Biosystems Cytofluor II fluorescent plate reader. Product
progress
curves were generated over 20 to 30 minutes following formation of AMC
product.
Inhibition studies
Potential inhibitors were evaluated using the progress curve method. Assays
were carried out in the presence of variable concentrations of test compound.
Reactions were initiated by addition of enzyme to buffered solutions of
inhibitor and
substrate. Data analysis was conducted according to one of two procedures
depending on the appearance of the progress curves in the presence of
inhibitors.
For those compounds whose progress curves were linear, apparent inhibition
constants (Ki~app) were calculated according to equation 1 (Brandt et al.,
Biochemistry, 1989, 28, 140):
v = VyiA l ~Ka(1 + IIKi~ app) +AJ
(1)
16
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where v is the velocity of the reaction with maximal velocity Vm , A is the
concentration of substrate with Michaelis constant of Ka, and I is the
concentration
of inhibitor.
For those compounds whose progress curves showed downward curvature
characteristic of time-dependent inhibition, the data from individual sets was
analyzed to give kobs according to equation 2:
[AMC) = vss t + w0 - vss) h - exP (-kobst)~ ~ kobs
(2)
where [AMC] is the concentration of product formed over time t, vp is the
initial
reaction velocity and vss is the final steady state rate. Values for kobs were
then
analyzed as a linear function of inhibitor concentration to generate an
apparent
second order rate constant (kobs / inhibitor concentration or kobs / [I))
describing
the time-dependent inhibition. A complete discussion of this kinetic treatment
has
been fully described (Morrison et al., Adv. Enzymol. Relat. Areas Mol. Biol.,
19$8,
61, 201).
This assay measures the affinity of inhibitors to cathepsin K. One skilled in
the art would consider any compound exhibiting a K; value of less than 50
micromolar to be a potential lead compound for further research.
Human Osteoclast Resorption Assay
Aliquots of osteoclastoma-derived cell suspensions were removed from
liquid nitrogen storage, warmed rapidly at 37°C and washed x 1 in RPMI-
1640
medium by centrifugation ( 1000 rpm, 5 min at 4°C). The medium was
aspirated and
replaced with murine anti-HLA-DR antibody, diluted 1:3 in RPMI-1640 medium,
and incubated for 30 min on ice The cell suspension was mixed frequently.
The cells were washed x2 with cold RPMI-1640 by centrifugation (1000
rpm, 5 min at 4°C) and then transferred to a sterile 15 rnL centrifuge
tube. The
number of mononuclear cells were enumerated in an improved Neubauer counting
chamber.
Sufficient magnetic beads (5 / mononuclear cell), coated with goat anti-mouse
IgG,
were removed from their stock bottle and placed into 5 mL of fresh medium
(this
washes away the toxic azide preservative). The medium was removed by
immobilizing the beads on a magnet and is replaced with fresh medium.
The beads were mixed with the cells and the suspension was incubated for 30
min on ice. The suspension was mixed frequently. The bead-coated cells were
immobilized on a magnet and the remaining cells (osteoclast-rich fraction)
were
17
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decanted into a sterile 50 mL centrifuge tube. Fresh medium was added to the
bead-
coated cells to dislodge any trapped osteoclasts. This wash process was
repeated
x10. The bead-coated cells were discarded.
The osteoclasts were enumerated in a counting chamber, using a large-bore
disposable plastic Pasteur pipette to charge the chamber with the sample. The
cells
were pelleted by centrifugation and the density of osteoclasts adjusted to
l.Sx104/mL in EMEM medium, supplemented with 10% fetal calf serum and
l.7glliter of sodium bicarbonate. 3 mL aliquots of the cell suspension ( per
treatment) were decanted into 15 mL centrifuge tubes. These cells were
pelleted by
1 o centrifugation. To each tube 3 mL, of the appropriate treatment was added
(diluted
to 50 uM in the EMEM medium). Also included were appropriate vehicle controls,
a
positive control (87MEMi diluted to 100 ug/mL) and an isotype control (IgG2a
diluted to 100 ug/mL). The tubes were incubate at 37°C for 30 min.
0.5 mL aliquots of the cells were seeded onto sterile dentine slices in a 48-
well plate and incubated at 37°C for 2 h. Each treatment was screened
in
quadruplicate. The slices were washed in six changes of warm PBS ( 10 mL /
well in
a 6-well plate) and then placed into fresh treatment or control and incubated
at 37°C
for 48 h. The slices were then washed in phosphate buffered saline and fixed
in 2%
glutaraldehyde (in 0.2M sodium cacodylate) for 5 min., following which they
were
washed in water and incubated in buffer for 5 min at 37°C. The slices
were then
washed in cold water and incubated in cold acetate buffer / fast red garnet
for 5 min
at 4°C. Excess buffer was aspirated, and the slices were air dried
following a wash
in water.
The TRAP positive osteoclasts were enumerated by bright-field microscopy
and were then removed from the surface of the dentine by sonication. Pit
volumes
were determined using the Nikon/Lasertec ILM21W confocal microscope.
EXAMPLE 3: Method of Detecting Inhibitors
The three dimensional atomic structure can be readily used as a template for
selecting potent inhibitors. Various computer programs and databases are
available
for the purpose. A good inhibitor should at least have excellent steric and
electrostatic complementarity to the target,. a fair amount of hydrophobic
surface
buried and sufficent conformational rigidity to minimize entropy loss upon
binding.
The approach usually comprises several steps:
1 ) Define a region to target. the active site cavity of procathepsin K can
be selected, but any place that is essential to the protease activity could
become a
18
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WO 00109b53 PCT/US98114634
potential target. Since the crystal structure has Been determined, the spatial
and
chemical properties of the target region is known.
2) Docking a small molecule onto the target. Many methods can be
used to archive this. Computer databases of three-dimensional structures are
available for screening millions of small molecular compounds. A negative
image
of these compounds can be calculated and used to match the shape of the target
cavity. The profiles of hydrogen bond donor-acceptor and lipophilic points of
these
compounds can also be used to complement those of the target. Anyone skilled
in
the art would be able to indentify many small molecules or fragments as hits.
3) Linking and extending recognzition fragments. Using the hits
identified by above procedure, one can incorporate different functional groups
or
small molecules into a single, larger molecule. The resulting molecule is
likely to be
more potent and have higher specificity. It is also possible to try to improve
the
"seed" inhibitor by adding more atoms or fragments that will interact with the
target
protein. The originally defined target region can be readily expanded to allow
further necessary extension.
A limited numer of promising compounds can be selected through the
process. They can then be synthesized and assayed for their inhibitory
properties.
The success rate can sometimes be as high as 20%, and it may still be higher
with
the rapid progresses in computing methods.
AbbrevaiationsE-64, (1-(N-[(L-3-traps-carboxyoxirane-2carbonyl)-
L-leucyl]amino]-4-guanidinobutane]
CBZ, benzyloxycarbonyl
AMC, aminomethylcoumarin
MPD, 2 methyl-2,4-pentanediol
PIPES, piperazone-N,N-bis(2-ethanesulfonic acid)
MES, 2-(N-morpholino)-ethanesulfonic acid
tris, tris(hydroxymethyl)-aminomethane
3o PEG, polyethyleneglycol
M, Molar
Rc = E~(Po - Fc)~ / Fo
Fo = observed structure amplitude
Fc = calculated structure amplitude
EDTA, ethylenediaminetetraacetic acid
DTT, 1,4-dithiothreitol
SDS-PAGE, sodium dodecylsulfate polyacrylamide gel electrophoresis
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This invention is not to be limited in scope by the specific embodiments
described herein. Indeed, various modifications of the invention in addition
to those
described herein will become apparent to those skilled in the art from the
foregoing
description. Such modifications are intended to fall within the scope of the
appended claims.
The disclosures of the patents, patent applications and publications cited
herein are incorporated by reference in their entireties.
CA 02301201 2000-02-04
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Table I
Table of the orthogonal three dimensional coordinates in
angstroms and B factors (~2) for procathepsin K.
Residue Atom X Y Z B
GLU CB 10.66 -1.63 54.79 15.00
5 GLU CG 10.16 -2.56 53.66 15.00
5 GLU CD 9.13 -3.60 54.12 15.00
5 GLU OE1 8.82 -3.66 55.34 15.00
5 GLU OE2 8.63 -4.36 53.25 15.00
5 GLU C 11.61 -1.76 57.08 15.00
5 GLU 0 11.20 -2.60 57.88 15.00
5 GLU N 11.93 -3.64 55.60 15.00
5 GLU CA 11.84 -2.15 55.63 15.00
6 ILE N 11.90 -0.50 57.39 15.00
6 ILE CA 11.75 0.09 58.73 15.00
6 ILE CB 12.62 -0.64 59.80 15.00
6 ILE CG2 11.83 -1.76 60.47 15.00
6 ILE CG1 13.96 -1.08 59.19 15.00
6 ILE CD1 14.78 -2.00 60.08 15.00
6 ILE C 12.20 1.55 58.67 15.00
6 ILE O 12.53 2.17 59.70 15.00
7 LEU N 12.15 2.12 57.47 15.00
7 LEU CA 12.57 3.50 57.23 15.00
7 LEU CB 12.18 3.97 55.84 15.00
7 LEU CG 12.66 3.11 .54.68 15.00
7 LEU CD1 12.56 3.91 53.40 15.00
7 LEU CD2 14.09 2.67 54.90 15.00
7 LEU C 12.09 4.50 58.26 15.00
7 LEU O 12.76 5.49 58.49 15.00
8 ASP N 10.95 4.24 58.89 15.00
8 ASP CA 10.46 5.19 59.88 15.00
8 ASP CB 9.09 4.80 60.45 15.00
8 ASP CG 8.27 6.03 60.89 15.00
8 ASP OD1 7.82 6.81 60.01 15.00
8 ASP OD2 8.09 6.23 62.12 15.00
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8 ASP C 11.48 5.28 61.00 15.00
8 ASP O 11.95 6.38 .61.35 15.00
9 THR N 11.91 4.13 61.50 15.00
9 THR CA 12.88 4.16 62.57 15.00
9 THR CB 13.03 2.79 63.24 15.00
9 THR OG1 13.87 2.91 64.39 15.00
9 THR CG2 13.60 1.76 62.26 15.00
9 THR C 14.24 4.69 62.10 15.00
9 THR O 14.83 5.57 62.74 15.00
10 HIS N 14.69 4.22 60.94 15.00
10 HIS CA 15.97 4.65 60.41 15.00
10 HIS CB 16.36 3.81 59.21 15.00
10 HIS CG 16.86 2.47 59.60 15.00
10 HIS CD2 17.15 1.96 60.82 15.00
10 HIS ND1 17.15 1.48 58.69 15.00
10 HIS CE1 17.60 0.41 59.34 15.00
10 HIS NE2 17.61 0.68 60.63 15.00
10 HIS C 16.15 6.12 60.11 15.00
10 HIS 0 17.18 6.68 60.45 15.00
11 TRP N 15.14 6.74 59.50 15.00
11 TRP CA 15.23 8.16 59.19 15.00
11 TRP CB 13.96 8.67 58.51 15.00
11 TRP CG 14.17 9.99 57.80 25.00
11 TRP CD2 13.47 11.22 58.04 15.00
11 TRP CE2 14.00 12.18 57.14 15.00
11 TRP CE3 12.46 11.60 58.93 15.00
11 TRP CD1 15.06 10.25 56.79 15.00
11 TRP NE1 14.97 11.56 56.39 15.00
11 TRP CZ2 13.54 13.50 57.10 15.00
11 TRP CZ3 12.00 12.91 58.89 15.00
11 TRP CH2 12.54 13.84 57.98 15.00
11 TRP C 15.47 8.94 60.48 15.00
11 TRP O 16.18 9.94 60.48 15.00
12 GLU N 14.93 8.43 61.58 15.00
12 GLU CA 15.11 9.06 62.88 15.00
12 GLU CB 14.29 8.34 63.95 15.00
12 GLU CG 14.34 9.01 65.32 15.00
22
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WU 00109653 PCT/US98/14634
12GLU CD 13.63 10.36 65.38 15.00
12GLU OE1 13.03 10.80 64.36 15.00
12GLU OE2 13.66 10.98 66.47 15.00
12GLU C 16.58 9.07 63.27 15.00
12GLU O 17.03 9.94 64.01 15.00
13LEU N 17.32 8.07 '62.81 15.00
13LEU CA 18.75 8.02 63.10 15.00
13LEU CB 19.33 6.62 62.88 15.00
13LEU CG 20.35 6.13 63.92 15.00
13LEU CD1 21.11 4.94 63.36 15.00
13LEU CD2 21.33 7.24 64.32 15.00
13LEU C 19.39 9.03 62.16 15.00
13LEU O 19.84 10.08 62.59 15.00
14TRP N 19.34 8.75 60.85 15.00
24TRP CA 19.91 9.62 59.$1 15.00
14TRP CB 19.27 9.31 58.46 15.00
14TRP CG 19.90 10.01 57.31 15.00
14TRP CD2 19.67 11.36 56.89 15.00
14TRP CE2 20.40 11.55 55.71 15.00
14TRP CE3 18.90 12.42 57.39 15.00
14TRP CD1 20.75 9.47 56.41 15.00
14TRP NE1 21.06 10.38 55.43 15.00
14TRP CZ2 20.40 12.77 55.02 15.00
14TRP CZ3 18.90 13.63 56.70 15.00
14TRP CH2 19.64 13.79 55.53 15.00
14TRP C 19.72 11.10 60.11 15.00
14TRP 0 20.60 11.91 59.82 15.00
15LYS N 18.55 11.46 60.63 15.00
15LYS CA 18.27 12.83 60.97 15.00
15LYS CB 16.86 12.96 61.54 15.00
15LYS CG 15.81 13.43 60.53 15.00
15LYS CD 14.44 13.50 61.18 15.00
15LYS CE 14.51 14.18 62.55 15.00
15LYS NZ 15.05 15.57 62.48 15.00
15LYS C 19.27 13.25 62.04 15.00
15LYS O 20.15 14.10 61.80 15.00
16LYS N 19.19 12.58 63.18 15.00
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16 LYS CA 20.05 12.86 64.31 15.00
16 LYS CB 19.56 12.12 65.55 15.00
16 LYS CG 19.67 10,62 65.47 15.00
26 LYS CD 18.71 9.97 66.46 15.00
16 LYS CE 18.98 8.49 66.61 15.00
16 LYS NZ 20.38 8.24 67.09 15.00
16 LYS C 21.52 12.55 64.09 15.00
16 LYS O 22.37 13.10 64.78 15.00
17 THR N 21.82 11.67 63.14 15.00
17 THR CA 23.20 11.31 .62.85 15.00
17 THR CB 23.36 10.18 61.82 15.00
17 THR OG1 22.32 9.22 61.98 15.00
17 THR CG2 24.71 9.49 62.02 15.00
17 THR C 23.85 12.54 62.25 15.00
17 THR O 25.07 12.65 62.18 15.00
18 HIS N 23.03 13.44 61.73 15.00
18 HIS CA 23.55 14.65 61.16 15.00
18 HIS CB 23.13 14.78 59.70 15.00
18 HIS CG 23.71 13.71 58.82 15.00
18 HIS CD2 24.64 13.77 57.84 15.00
18 HIS ND1 23.32 12.40 58.90 15.00
18 HIS CE1 23.99 11.69 58.01 15.00
18 HIS NE2 24.80 12.50 57.35 15.00
18 HIS C 22.98 15.77 62.02 15.00
18 HIS O 22.58 15.51 .63.16 15.00
19 ARG N 22.98 16.99 61.51 15.00
19 ARG CA 22.45 18.13 62.26 15.00
19 ARG CB 23.41 19.33 62.19 15.00
19 ARG CG 24.11 19.55 60.83 15.00
19 ARG CD 25.62 19.36 60.95 15.00
19 ARG NE 26.28 20.55 61.49 15.00
19 ARG CZ 27.60 20.71 61.62 15.00
19 ARG NH1 28.08 21.85 62.10 15.00
19 ARG NH2 28.44 19.71 61.32 15.00
19 ARG C 21.05 18.50 61.74 15.00
19 ARG O 20.65 19.67 61.76 15.00
20 LYS N 20.32 17.49 61.27 15.00
24
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20LYS CA 18.99 17.69 60.74 15.00
20LYS CB 18.53 16.48 59.93 15.00
20LYS CG 19.50 16.05 58.86 15.00
20LYS CD 19.70 17.13 57.84 15.00
20LYS CE 20.67 16.65 56.78 15.00
20LYS NZ 20.71 17.52 55.56 15.00
20LYS C 18.02 17.91 61.89 15.00
20LYS O 17.88 17.06 62.78 15.00
21GLN N 17.42 19.09 61.90 15.00
21GLN CA 16.45 19.47 62.91 15.00
21GLN CB 17.16 20.10 64.12 15.00
21GLN CG 16.35 20.08 65.40 15.00
21GLN CD 16.09 18.67 65.97 15.00
21GLN OE1 15.74 18.53 67.14 15.00
21GLN NE2 16.27 17.63 65.14 15.00
21GLN C 15.69 20.49 62.11 15.00
21GLN 0 16.13 21.63 61.96 15.00
22TYR N 14.62 20.02 61.47 15.00
22TYR CA 13.80 20.86 60.60 15.00
22TYR CB 12.98 20.00 59.65 15.00
22TYR CG 13.82 18.98 58.92 15.00
22TYR CD1 14.37 17.89 59.59 15.00
22TYR CE1 15.08 16.91 58.91 15.00
22TYR CD2 14.02 19.07 57.54 15.00
22TYR CE2 14.72 18.10 56.86 15.00
22TYR CZ 15.25 17.02 57.55 15.00
22TYR OH 15.92 16.05 56.86 15.00
22TYR C 12.88 21.73 61.43 15.00
22TYR 0 12.54 21.37 62.56 15.00
23ASN N 12.48 22.87 60.88 15.00
23ASN CA 11.62 23.77 61.63 15.00
23ASN CB 11.83 25.24 61.22 15.00
23ASN CG 11.47 25.52 59.76 15.00
23ASN OD1 10.44 25.07 59.26 15.00
23ASN ND2 12.34 26.26 59.07 15.00
23ASN C 10.13 23.43 61.61 15.00
23ASN 0 9.34 24.13 62.25 15.00
25
CA 02301201 2000-02-04
WO 00!09653 PCT/US98/14634
24 ASN N 9.76 22.35 60.91 15.00
24 ASN CA 8.35 21.95 60.81 15.00
24 ASN CB 7.55 23.04 60.11 15.00
24 ASN CG 6.49 22.48 59.19 15.00
24 ASN OD1 5.48 21.92 59.65 15.00
24 ASN ND2 6.73 22.57 57.89 15.00
24 ASN C 8.11 20.62 60.07 15.00
24 ASN 0 8.84 20.29 59.13 15.00
25 LYS N 7.00 19.96 60.40 15.00
25 LYS CA 6.62 18.68 59.79 15.00
25 LYS CB 5.46 18.01 60.53 15.00
25 LYS CG 5.87 17.19 61.76 15.00
25 LYS CD 5.71 17.97 63.06 15.00
25 LYS CE 6.32 17.20 64.25 15.00
25 LYS NZ 7.84 17.31 64.32 15.00
25 LYS C 6.29 18.75 58.31 15.00
25 LYS O 6.55 17.81 57.58 15.00
26 VAL N 5.67 19.84 57.86 15.00
26 VAL CA 5.34 19.92 .56.44 15.00
26 VAL CB 4.57 21.24 56.06 15.00
26 VAL CG1 4.44 21.39 54.55 15.00
26 VAL CG2 3.17 21.22 56.68 15.00
26 VAL C 6.66 19.78 55.68 15.00
26 VAL O 6.74 19.01 54.72 15.00
27 ASP N 7.71 20.44 56.17 15.00
27 ASP CA 9.02 20.33 55.53 15.00
27 ASP CB 10.04 21.31 56.10 15.00
27 ASP CG 9.46 22.65 56.38 15.00
27 ASP OD1 9.75 23.18 57.48 15.00
27 ASP OD2 8.71 23.18 55.52 15.00
27 ASP C 9.50 18.93 55.84 15.00
27 ASP O 9.69 18.12 54.93 15.00
28 GLU N 9.65 18.64 57.13 15.00
28 GLU CA 10.12 17.34 .57.58 15.00
28 GLU CB 9.66 17.07 59.01 15.00
28 GLU CG 10.34 15.87 59.63 15.00
28 GLU CD 10.44 15.93 61.15 15.00
26
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
28GLU OE1 10.03 16.95 61.74 15.00
28GLU OE2 10.94 14.95 61.74 15.00
28GLU C 9.64 16.24 56.65 15.00
28GLU O 10.43 15.68 55.89 15.00
29ILE N 8.33 16.04 56.59 15.00
29ILE CA 7.73 15.02 55.74 15.00
29ILE CB 6.16 15.07 55.73 15.00
29ILE CG2 5.60 13.77 55.16 15.00
29ILE CG1 5.56 15.32 '57.13 15.00
29ILE CD1 6.01 14.36 58.22 15.00
29ILE C 8.20 15.19 54.29 15.00
29ILE O 8.50 14.21 53.62 15.00
30SER N 8.29 16.44 53.83 15.00
30SER CA 8.69 16.70 52.44 15.00
30SER CB 8.47 18.17 52.07 15.00
30SER OG 9.56 18.98 52.47 15.00
30SER C 10.13 16.33 52.18 15.00
30SER O 10.44 15.77 51.12 15.00
31ARG N 11.02 16.66 53.12 15.00
31ARG CA 12.45 16.36 52.99 15.00
31ARG CB 13.21 16.98 54.15 15.00
31ARG CG 12.99 18.47 54.28 15.00
31ARG CD 14.11 19.29 53.67 15.00
31ARG NE 14.07 19.35 52.21 15.00
31ARG CZ 14.49 20.39 51.51 15.00
31ARG NH1 14.98 21.46 52.13 15.00
31ARG NH2 14.44 20.38 50.18 15.00
31ARG C 12.57 24.85 53.05 15.00
31ARG O 13.27 14.24 52.25 15.00
32ARG N 11.81 14.27 53.97 15.00
32ARG CA 11.75 12.83 54.16 15.00
32ARG CB 10.79 12.54 55.31 15.00
32ARG CG 10.21 11.16 55.32 15.00
32ARG CD 11.27 10.14 55.59 15.00
32ARG NE 10.70 8.80 55.66 15.00
32ARG CZ 10.00 8.36 56.70 15.00
32ARG NH1 9.79 9.14 57.76 15.00
27
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
32 ARG NH2 9.48 7.13 56.66 15.00
32 ARG C 11.29 12.12 52.88 15.00
32 ARG O 11.68 10.98 52.63 15.00
33 LEU N 10.47 12.78 52.08 15.00
33 LEU CA 10.01 12.18 50.83 15.00
33 LEU CB 8.74 12.85 50.31 15.00
33 LEU CG 7.83 11.79 49.70 15.00
33 LEU CD1 6.64 11.61 50.61 15.00
33 LEU CD2 7.42 12.15 48.27 15.00
33 LEU C 11.10 12.25 49.79 15.00
33 LEU O 11.16 11.41 48.90 15.00
34 ILE N 11.93 13.29 49.88 15.00
34 ILE CA 13.05 13.43 48.96 15.00
.
34 ILE CB 13.71 14.81 49.07 15.00
34 ILE CG2 14.67 14.99 47.92 15.00
34 ILE CG1 12.67 15.92 48.96 15.00
34 ILE CD1 13.24 17.31 49.09 15.00
34 ILE C 14.07 12.36 49.36 15.00
34 ILE O 14.56 12.59 48.52 15.00
35 TRP N 14.32 12.27 50.66 15.00
35 TRP CA 15.25 11.29 51.22 15.00
35 TRP CB 15.08 11.24 52.73 15.00
35 TRP CG 15.93 10.24 53.44 15.00
35 TRP CD2 15.53 8.95 53.90 15.00
35 TRP CE2 16.58 8.45 54.70 15.00
35 TRP CE3 14.38 8.17 53.73 15.00
35 TRP CD1 17.18 10.45 53.94 15.00
35 TRP NE1 17.57 9.39 54.70 15.00
35 TRP CZ2 16.51 7.21 55.33 15.00
35 TRP CZ3 14.32 6.93 54.37 15.00
35 TRP CH2 15.37 6.47 55.16 15.00
35 TRP C 14.91 9.94 50.64 15.00
35 TRP O 15.78 9.22 50.15 15.00
36 GLU N 13.62 9.62 50.66 15.00
36 GLU CA 13.12 8.37 50.15 15.00
36 GLU CB 11.65 8.21 50.51 15.00
36 GLU CG 11.38 8.22 52.01 15.00
28
CA 02301201 2000-02-04
WO 00/09653 PCT/US98114634
36 GLU CD 10.29 7.27 52.42 15.00
36 GLU OE1 9.95 7.27 53.62 15.00
36 GLU OE2 9.79 6.50 '51.56 15.00
36 GLU C 13.33 8.22 48.65 15.00
36 GLU O 13.63 7.13 48.17 15.00
37 LYS N 13.17 9.30 47.90 15.00
37 LYS CA 13.37 9.25 46.45 15.00
37 LYS CB 13.06 10.59 45.79 15.00
37 LYS CG 11.56 10.88 45.66 15.00
37 LYS CD 11.27 12.10 44.78 15.00
37 LYS CE 11.74 13.38 45.46 15.00
37 LYS NZ 11.14 13.54 46.83 15.00
37 LYS C 14.80 8.83 46.13 15.00
37 LYS 0 15.03 7.80 45.48 15.00
38 ASN N 15.76 9.61 46.66 15.00
38 ASN CA 17.19 9.38 46.48 15.00
38 ASN CB 18.00 10.45 47.19 15.00
38 ASN CG 17.81 11.81 '46.58 15.00
38 ASN OD1 17.65 12.94 45.36 15.00
38 ASN ND2 17.84 12.84 47.42 15.00
38 ASN C 17.63 8.02 46.98 15.00
38 ASN O 18.41 7.34 46.31 15.00
39 LEU N 17.18 7.64 48.18 15.00
39 LEU CA 17.53 6.34 48.76 15.00
39 LEU CB 16.73 6.07 50.05 15.00
39 LEU CG 17.36 5.16 51.11 15.00
39 LEU CD1 16.40 4.97 52.27 15.00
39 LEU CD2 17.71 3.80 50.53 15.00
39 LEU C 17.15 5.30 47.71 15.00
39 LEU O 17.96 4.44 47.33 15.00
40 LYS N 15.95 5.46 47.18 15.00
40 LYS CA 15.43 4.57 46.16 15.00
40 LYS CB 13.99 4.99 45.83 15.00
40 LYS CG 13.13 3.90 45.22 15.00
40 LY'SCD 11.66 4.08 45.59 15.00
40 LYS CE 11.12 5.44 45.17 15.00
40 LYS NZ 11.27 5.65 43.70 15.00
29
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
40 LYS C 16.34 4.65 44.94 15.00
40 LYS 0 16.71 3.63 44.36 15.00
41 TYR N 16.75 5.87 44.60 15.00
41 TYR CA 17.60 6.11 43.44 15.00
41 TYR CB 17.90 7.61 43.27 15.00
41 TYR CG 18.84 7.87 42.12 15.00
41 TYR CD1 20.14 8.32 42.34 15.00
41 TYR CE1 21.06 8.39 ,41.28 15.00
41 TYR CD2 18.46 7.53 40.82 15.00
41 TYR CE2 19.36 7.60 39.76 15.00
41 TYR CZ 20.66 8.02 39.99 15.00
41 TYR OH 21.56 7.98 38.95 15.00
41 TYR C 18.92 5.36 43.57 15.00
41 TYR O 19.40 4.76 42.61 15.00
42 ILE N 19.49 5.42 44.76 15.00
42 ILE CA 20.76 4.77 45.03 15.00
42 ILE CB 21.22 5.07 46.46 15.00
42 ILE CG2 22.50 4.32 46.76 15:00
42 ILE CG1 21.45 6.57 46.63 15.00
42 ILE CD1 21.94 6.97 47.99 15.00
42 ILE C 20.63 3.28 44.85 15.00
42 ILE O 21.25 2.71 43.97 15.00
43 SER N 19.75 2.68 .45.64 15.00
43 SER CA 19.48 1.25 45.64 15.00
43 SER CB 18.16 0.97 46.38 15.00
43 SER OG 17.89 -0.41 46.51 15.00
43 SER C 19:46 0.60 44.26 15.00
43 SER 0 19.91 -0.54 44.11 15.00
44 ILE N 18.98 2.31 43.26 15.00
44 ILE CA 18.91 0.79 41.90 15.00
44 ILE CB 17.78 1.47 41.08 15.00
44 ILE CG2 17.85 1.04 39.63 15.00
44 ILE CG1 16.41 1.14 41.69 15.00
44 ILE CD1 15.33 2.08 41.25 15.00
44 ILE C 20.22 0.99 41.17 15.00
44 ILE O 20.65 0.13 40.40 15.00
45 HIS N 20.82 2.16 41.37 15.00
30
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
45HIS CA 22.09 2.49 40.73 15.00
45HIS CB 22.54 3.90 41.14 15.00
45HIS CG 23.66 4.46 40.30 15.00
45HIS CD2 23.65 5.22 39.19 15.00
45HIS ND1 25.00 4.24 40.60 15.00
45HIS CE1 25.75 4.86 39.70 15.00
45HIS NE2 24.96 5.46 38.84 15.00
45HIS C 23.11 1.43 41.16 15.00
45HIS O 23.80 0.85 40.32 15.00
46ASN N 23.18 1.15 42.45 15.00
46ASN CA 24.11 0.14 42.93 15.00
46ASN CB 24.06 0.01 44.45 15.00
46ASN CG 24.80 1.13 45.15 15.00
46ASN OD1 25.23 2.09 44.52 15.00
46ASN ND2 24.95 1.01 46.44 15.00
46ASN C 23.77 -1.19 42.26 15.00
46ASN O 24.65 -1.87 41.75 15.00
47LEU N 22.48 -1.55 42.22 15.00
47LEU CA 22.07 -2.79 41.58 15.00
47LEU CB 20.53 -2.86 41.50 15.00
47LEU CG 19.85 -4.07 40.82 15.00
47LEU CD1 20.08 -5.34 41.62 15.00
47LEU CD2 18.36 -3.80 40.71 15.00
47LEU C 22.66 -2.80 40.17 15.00
47LEU O 23.32 -3.76 39.79 15.00
48GLU N 22.48 -1.71 39.43 15.00
48GLU CA 23.01 -1.62 38.07 15.00
.
48GLU CB 22.68 -0.26 37.42 15.00
48GLU CG 21.19 0.09 37.26 15.00
48GLU CD 20.95 1.39 36.46 15.00
48GLU OE1 20.57 2.43 37.06 15.00
48GLU OE2 21.13 1.37 35.22 15.00
48GLU C 24.52 -1.84 38.09 15.00
48GLU 0 25.08 -2.40 37.14 15.00
49ALA N 25.18 -1.41 39.16 15.00
49ALA CA 26.64 -1.57 39.31 15.00
49ALA CB 27.18 -0.75 40.48 15.00
31
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
49 ALA C 27.03 -3.04 39.48 15.00
49 ALA O 28.07 -3.45 38.98 15.00
50 SER N 26.22 -3.81 40.19 15.00
50 SER CA 26.48 -5.23 40.38 15.00
50 SER CB 25.41 -5.86 41.28 15.00
50 SER OG 24.70 -4.87 42.02 15.00
SO SER C 26.43 -5.91 39.02 15.00
50 SER 0 26.98 -6.99 38.81 15.00
51 LEU N 25.69 -5.29 38.10 15.00
51 LEU CA 25.58 -5.82 36.76 15.00
51 LEU CB 24.17 -5.61 36.20 15.00
51 LEU CG 23.04 -6.48 36.74 15.00
51 LEU CD1 23.53 -7.90 36.84 15.00
51 LEU CD2 22.54 -5.99 38.09 15.00
51 LEU C 26.63 -5.11 35.91 15.00
51 LEU 0 26.61 -5.15 34.67 15.00
52 GLY N 27.51 -4.39 36.60 15.00
52 GLY CA 28.60 -3.68 35.96 15.00
52 GLY C 28.37 -2.64 34.88 15.00
52 GLY O 29.30 -2.32 34.15 15.00
53 VAL N 27.16 -2.11 34.77 25.00
53 vAL cA 26.91 -1.09 33.74 15.00
53 VAL CB 25.43 -1.05 33.33 15.00
53 VAL CG1 25.06 -2.34 32.62 15.00
53 VAL CG2 24.54 -0.83 34.53 15.00
53 VAL C 27.42 0.30 34.15 15.00
53 VAL O 27.41 1.24 33.35 15.00
54 HIS N 27.88 0.41 35.40 15.00
54 HIS CA 28.43 1.66 35.94 15.00
54 HIS CB 27.55 2.19 37.06 15.00
54 HIS CG 26.18 2.62 36.61 15.00
54 HIS CD2 25.05 2.86 .37.30 15.00
54 HIS ND1 25.87 2.83 35.29 15.00
54 HIS CE1 24.60 3.18 35.18 15.00
54 HIS NE2 24.08 3.21 36.40 15.00
54 HIS C 29.79 1.27 36.51 15.00
54 HIS 0 30.05 0.07 36.66 15.00
32
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
55 THR N 30.69 2.23 36.76 15.00
55 THR CA 31.98 1.87 37.34 15.00
55 THR CB 33.17 2.13 36.41 15.00
55 THR OG1 33.23 3.51 36.08 15.00
55 THR CG2 33.04 1.30 35.15 15.00
55 THR C 32.23 2.55 38.68 15.00
55 THR O 33.37 2.63 39.15 15.00
56 TYR N 31.14 3.00 39.29 15.00
56 TYR CA 31.17 3.67 40.59 15.00
56 TYR CB 31.14 5.18 40.44 15.00
56 TYR CG 30.10 5.69 39.48 15.00
56 TYR CD1 29.01 6.42 39.93 15.00
56 TYR CE1 28.06 6.87 39.05 15.00
56 TYR CD2 30.21 5.42 38.12 15.00
56 TYR CE2 29.26 5.86 37.23 15.00
56 TYR CZ 28.18 6.58 37.70 15.00
56 TYR OH 27.23 6.98 36.80 15.00
56 TYR C 29.95 3.20 41.36 15.00
56 TYR O 29.05 2.58 40.80 15.00
57 GLU N 29.93 3.51 42.65 15.00
57 GLU CA 28.84 3.12 43.53 15.00
57 GLU CB 29.25 1.87 .44.31 15.00
57 GLU CG 29.73 0.76 43.38 15.00
57 GLU CD 30.28 -0.45 44.10 15.00
57 GLU OE1 30.16 -1.56 43.52 15.00
57 GLU OE2 30.84 -0.30 45.21 15.00
57 GLU C 28.51 4.29 44.46 15.00
57.GLU O 29.41 5.06 44.85 15.00
58 LEU N 27.22 4.43 44.79 15.00
58 LEU CA 26.71 5.51 45.62 15.00
58 LEU CB 25.41 6.03 45.03 15.00
58 LEU CG 25.33 6.69 43.65 15.00
58 LEU CD1 26.48 6.32 42.76 15.00
58 LEU CD2 24.02 6.29 43.02 15.00
58 LESTC 26.42 5.08 47.04 15.00
58 LEU O 26.37 3.88 47.34 15.00
59 ALA N 26.18 6.06 47.91 15.00
33
CA 02301201 2000-02-04
WO 00109653 PCT/US98/14634
59 ALA CA 25.85 5.82 49.31 15.00
59 ALA CB 27.08 5.49 50.11 15.00
59 ALA C 25.16 7.07 49.86 15.00
59 ALA O 25.24 8.13 49.25 15.00
60 MET N 24.48 6.95 50.98 15.00
60 MET CA 23.75 8.07 51.56 15.00
60 MET CB 22.55 7.54 52.34 15.00
60 MET CG 21.83 8.51 53.27 15.00
60 MET SD 20.90 9.80 52.48 15.00
60 MET CE 20.32 8.94 51.04 15.00
60 MET C 24.62 9.01 52.40 15.00
60 MET O 25.05 8.69 .53.50 15.00
62 ASN N 24.85 10.20 51.85 15.00
61 ASN CA 25.66 11.22 52.49 15.00
61 ASN CB 26.54 11.87 51.42 15.00
61 ASN CG 25.73 12.41 50.29 15.00
61 ASN OD1 24.80 13.17 50.50 15.00
61 ASN ND2 26.05 11.98 49.08 15.00
61 ASN C 24.78 12.29 53.16 15.00
61 ASN O 23.56 12.26 53.02 15.00
62 HIS N 25.40 13.25 53.86 15.00
62 HIS CA 24.64 14.31 54.55 15.00
62 HIS CB 25.58 15.21 55.37 15.00
62 HIS CG 26.57 16.01 54.57 15.00
62 HIS CD2 27.33 17.08 54.91 15.00
62 HIS ND1 26.89 15.72 53.26 15.00
.
62 HIS CE1 27.81 16.57 52.83 15.00
62 HIS NE2 28.09 17.41 53.82 15.00
62 HIS C 23.78 15.19 53.63 15.00
62 HIS O 23.40 16.30 54.01 15.00
63 LEU N 23.51 14.73 52.41 15.00
63 LEU CA 22.71 15.49 51.47 15.00
63 LEU CB 23.58 15.96 50.30 15.00
63 LEU CG 24.91 16.63 50.64 15.00
63 LEU CD1 25.52 17.18 49.38 15.00
63 LEU CD2 24.70 17.72 51.64 15.00
63 LEU C 21.56 14.61 50.98 15.00
34
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
63LEU O 21.18 14.65 .49.81 15.00
64GLY N 21.02 13.79 51.87 15.00
64GLY CA 19.91 12.93 51.52 25.00
64GLY C 18.67 13.77 51.25 15.00
64GLY O 18.14 13.73 50.14 25.00
65ASP N 28.27 14.57 52.23 15.00
65ASP CA 17.08 15.43 52.13 15.00
65ASP CB 16.64 15.92 53.51 15.00
65ASP CG 17.70 16.74 54.24 15.00
65ASP OD1 17.88 16.50 55.45 15.00
65ASP OD2 18.34 17.66 53.66 15.00
65ASP C 17.23 16.61 51.19 15.00
65ASP O 16.84 17.71 51.52 15.00
66MET N 17.74 16.38 50.00 15.00
66MET CA 17.93 17.45 49.05 15.00
66MET CB 19.28 18.16 49.28 15.00
66MET CG 19.43 18.98 50.57 15.00
66MET SD 20.95 20.02 50.58 15.00
66MET CE 21.46 19.92 52.32 15.00
66MET C 17.97 16.77 47.70 15.00
66MET O 17.69 15.58 47.60 15.00
67THR N 18.35 17.51 46.67 15.00
67THR CA 18.49 16.96 45.34 15.00
67THR CB 17.13 16.$4 44.60 15.00
67THR OG1 17.35 26.31 43.28 15.00
67THR CG2 16.43 18.20 44.52 15.00
67THR C 19.45 17.82 44.52 15.00
67THR 0 19.75 18.96 44.90 15.00
68SER N 19.88 17.27 43.40 15.00
68SER CA 20.80 17.91 42.47 15.00
68SER CB 20.81 17.13 '42.17 15.00
68SER OG 19.54 16.53 40.96 15.00
68SER C 20.58 19.39 42.18 15.00
68SER O 21.54 20.14 42.07 15.00
69GLU N 19.33 19.81 42.03 15.00
69GLU CA 19.05 21.22 41.75 15.00
69GLU CB 17.59 21.44 41.33 15.00
35
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
69 GLU CG 17.26 22.91 41.02 15.00
69 GLU CD 15.77 23.27 41.20 15.00
69 GLU OE1 15.47 24.38 41.69 15.00
69 GLU OE2 14.90 22.44 40.84 15.00
69 GLU C 19.36 22.06 42.98 15.00
69 GLU 0 19.78 23.21 42.87 15.00
70 GLU N 19.20 21.48 44.17 15.00
70 GLU CA 19.46 22.19 45.41 15.00
70 GLU CB 18.66 21.56 46.53 15.00
70 GLU CG 17.18 21.54 46.17 15.00
70 GLU CD 16.27 21.05 47.26 15.00
70 GLU OE1 16.05 21.80 48.24 15.00
70 GLU OE2 15.74 19.92 47.11 15.00
70 GLU C 20.94 22.31 45.73 15.00
70 GLU O 21.44 23.40 46.03 15.00
71 VAL N 21.67 21.21 45.62 15.00
71 VAL CA 23.10 21.24 45.89 15.00
71 VAL CB 23.78 19.86 45.70 25.00
71 VAL CG1 23.19 18.83 46.65 15.00
71 VAL CG2 23.64 19.39 44.27 15.00
71 VAL C 23.70 22.24 44.93 15.00
71 VAL O 24.53 23.06 45.31 15.00
72 VAL N 23.24 22.21 43.69 15.00
72 VAL CA 23.75 23.12 42.70 15.00
72 VAL CB 23.34 22.69 41.28 15.00
72 VAL CG1 23.70 23.75 40.28 15.00
72 VAL CG2 24.02 21.39 40.91 15.00
72 VAL C 23.27 24.53 42.99 15.00
72 VAL O 23.97 25.48 42.68 15.00
73 GLN N 22.12 24.66 43.64 15.00
73 GLN CA 21.58 26.00 43.92 15.00
73 GLN CB 20.07 25.95 44.18 15.00
73 GLN CG 19.40 27.35 44.23 15.00
73 GLN CD 18.27 27.46 45.24 15.00
73 GLN OE1 17.55 26.49 .45.51 15.00
73 GLN NE2 18.11 28.65 45.83 15.00
73 GLN C 22.24 26.70 45.09 15.00
36
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
73GLN 0 22.43 27.90 45.05 15.00
74LYS N 22.55 25.96 46.15 15.00
74LYS CA 23.15 26.60 47.31 15.00
74LYS CB 22.15 26.59 48.48 15.00
74LYS CG 21.46 25.25 48.73 15.00
74LYS CD 20.41 25.32 49.88 15.00
74LYS CE 19.11 26.03 49.46 15.00
74LYS NZ 18.34 25.38 48.33 15.00
74LYS C 24.53 26.07 47.77 15.00
74LYS O 25.00 26.43 .48.86 15.00
75MET N 25.19 25.27 46.93 15.00
75MET CA 26.49 24.70 47.29 15.00
75MET CB 26.30 23.34 47.96 15.00
75MET CG 25.56 23.38 49.25 15.00
75MET SD 25.38 21.74 49.87 15.00
75MET CE 26.23 21.91 51.38 15.00
75MET C 27.52 24.57 46.17 15.00
75MET 0 28.31 23.63 46.18 15.00
76THR N 27.50 25.45 45.19 15.00
76THR CA 28.48 25.45 44.12 15.00
76THR CB 27.97 24.75 42.84 15.00
76THR OG1 26.90 25.50 42.28 15.00
76THR CG2 27.50 23.34 43.14 15.00
76THR C 28.74 26.93 43.86 15.00
76THR O 28.92 27.68 .44.82 15.00
77GLY N 28.75 27.36 42.60 15.00
77GLY CA 28.96 28.77 42.33 15.00
77GLY C 30.07 29.15 41.36 15.00
77GLY O 30.68 30.20 41.51 15.00
78LEU N 30.32 28.33 40.35 15.00
78LEU CA 31.38 28.66 39.42 15.00
78LEU CB 31.85 27.45 38.61 15.00
78LEU CG 33.00 27.84 37.67 15.00
78LEU CD1 34.30 27.78 38.44 15.00
78LEL1CD2 33.07 26.95 36.46 15.00
78LEU C 30.86 29.69 38.47 15.00
78LEU O 30.29 29.33 37.44 15.00
37
CA 02301201 2000-02-04
WO 00/09653 PCT/US98114634
79LYS N 31.00 30.96 38.81 15.00
79LYS CA 30.57 32.02 37.93 15.00
79LYS CB 30.77 33.37 38.60 15.00
79LYS CG 29.87 34.48 38.12 15.00
79LYS CD 28.54 34.41 38.82 15.00
79LYS CE 27.78 35.74 38.71 15.00
79LYS NZ 28.35 36.82 39.58 15.00
79LYS C 31.52 31.90 36.75 15.00
79LYS O 32.58 32.52 36.75 15.00
80VAL N 31.18 31.04 35.78 15.00
80VAL CA 32.02 30.81 34.61 15.00
80VAL CB 31.23 30.06 33.49 15.00
80VAL CG1 30.41 31.00 32.66 15.00
80VAL CG2 32.14 29.23 32.63 15.00
80VAL C 32.57 32.17 34.16 15.00
80VAL 0 31.87 33.19 34.25 15.00
81PRO N 33.84 32.21 33.74 15.00
81PRO CD 34.68 31.03 33.50 15.00
81PRO CA 34.53 33.42 33.29 15.00
81PRO CB 35.96 32.92 33.09 15.00
81PRO CG 35.73 31.56 32.54 15.00
81PRO C 34.00 34.12 32.03 15.00
81PRO O 32.79 34.26 31.82 15.00
82LEU N 34.96 34.60 31.23 15.00
82LEU CA 34.73 35.30 29.97 15.00
82LEU CB 34.38 36.77 30.24 15.00
82LEU CG 34.75 37.85 29.22 15.00
82LEU CD1 33.96 37.69 27.91 15.00
82LEU CD2 34.52 39.22 29.85 15.00
82LEU C 36.07 35.17 .29.23 15.00
82LEU O 37.05 34.72 29.83 15.00
83SER N 36.12 35.54 27.95 15.00
83SER CA 37.35 35.45 27.17 15.00
83SER CB 37.17 36.03 25.77 15.00
83SEA OG 36.98 34.98 24.81 15.00
83SER C 38.68 35.92 27.78 15.00
83SER O 38.78 37.02 28.37 15.00
38
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
84 HIS N 39.67 35.05 27.61 15.00
84 HIS CA 41.04 35.22 28.07 15.00
84 HIS CB 41.09 35.25 29.61 15.00
84 HIS CG 42.39 35.75 30.18 15.00
84 HIS CD2 42.72 36.94 30.75 15.00
84 HIS ND1 43.55 34.99 30.19 25.00
84 HIS CE1 44.52 35.69 30.75 15.00
84 HIS NE2 44.05 36.88 31.09 15.00
84 HIS C 41.90 34.05 27.56 15.00
84 HIS O 42.85 33.65 28.23 15.00
85 SER N 41.54 33.48 26.41 15.00
85 SER CA 42.33 32.37 25.84 15.00
85 SER CB 42.43 31.19 26.83 15.00
85 SER OG 43.22 30.13 26.30 15.00
85 SER C 41.91 31.83 24.46 15.00
85 SER O 40.73 31.77 24.12 15.00
86 ARG N 42.92 31.36 23.73 15.00
86 ARG CA 42.74 30.80 22.39 15.00
86 ARG CB 42.47 31.93 21.36 15.00
86 ARG CG 41.83 31.46 20.04 15.00
86 ARG CD 40.35 31.05 20.25 15.00
86 ARG NE 39.78 30.29 19.13 15.00
86 ARG CZ 39.86 30.63 17.84 15.00
86 ARG NH1 40.50 31.73 17.46 15.00
86 ARG NH2 39.30 29.84 16.91 15.00
86 ARG C 44.03 30.02 22.03 15.00
86 ARG O 44.51 30.07 20.90 15.00
87 SER N 44.60 29.36 23.03 15.00
87 SER CA 45.80 28.55 22.84 15.00
87 SER CB 45.48 27.28 22.04 15.00
87 SER OG 44.46 26.51 22.68 15.00
87 SER C 47.06 29.24 22.27 15.00
87 SER O 47.65 28.76 21.30 15.00
88 ASN N 47.47 30.35 22.88 15.00
.
88 ASN CA 48.68 31.07 22.43 15.00
88 ASN CB 48.65 31.36 20.90 15.00
88 ASN CG 47.56 32.38 20.49 15.00
39
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
88ASN OD1 46.95 33.06 21.33 15.00
88ASN ND2 47.33 32.49 19.18 15.00
88ASN C 48.96 32.35 23.21 15.00
88ASN O 49.83 33.15 22.85 15.00
89ASP N 48.24 32.53 24.32 15.00
89ASP CA 48.40 33.72 25.14 15.00
89ASP CB 47.28 33.81 26.18 15.00
89ASP CG 46.34 34.97 25.92 15.00
89ASP OD1 46.63 36.09 .26.41 15.00
89ASP OD2 45.31 34.76 25.24 15.00
89ASP C 49.75 33.75 25.85 15.00
89ASP O 50.62 32.93 25.58 15.00
90THR N 49.87 34.71 26.76 15.00
90THR CA 51.07 34.91 27.57 15.00
90THR CB 51.26 36.42 27.89 15.00
90THR OG1 50.27 36.83 28.83 15.00
90THR CG2 51.10 37.26 26.62 15.00
90THR C 50.84 34.14 28.87 15.00
90THR O 51.75 33.52 29.41 15.00
91LEU N 49.61 34.23 29.39 25.00
91LEU CA 49.23 33.52 30.60 15.00
91LEU CB 48.31 34.38 31.47 15.00
91LEU CG 48.82 34.78 32.87 15.00
91LEU CD1 50.36 34.84 .32.93 15.00
91LEU CD2 48.21 36.13 33.25 15.00
91LEU C 48.48 32.31 30.08 15.00
91LEU O 47.26 32.29 30.04 15.00
92TYR N 49.24 31.31 29.65 15.00
92TYR CA 48.71 30.09 29.06 15.00
92TYR CB 48.45 30.39 27.59 15.00
92TYR CG 48.08 29.20 26.76 15.00
92TYR CD1 49.05 28.36 26.26 15.00
92TYR CE1 48.73 27.27 25.45 15.00
92TYR CD2 46.74 28.95 26.43 15.00
92TYR CE2 46.40 27.87 25.63 15.00
92TYR CZ 47.40 27.03 25.14 15.00
92TYR OH 47.09 25.97 24.30 15.00
40
CA 02301201 2000-02-04
WO 00/09653 PCT1US98/14634
92TYR C 49.84 29.07 29.22 15.00
92TYR O 51.00 29.41 29.01 15.00
93ILE N 49.53 27.82 29.57 15.00
93ILE CA 50.61 26.86 29.78 15.00
93ILE CB 50.50 26.15 31.14 15.00
93ILE CG2 49.45 25.09 31.09 15.00
93ILE CG1 51.80 25.44 31.46 15.00
93ILE CD1 51.84 24.86 32.84 15.00
93ILE C 50.90 25.82 28.72 15.00
93ILE 0 50.02 25.09 28.30 15.00
94PRO N 52.12 25.85 28.18 15.00
94PRO CD 52.88 27.11 28.07 15.00
94PRO CA 52.60 24.92 27.16 15.00
94PRO CB 53.31 25.83 26.15 15.00
94PRO CG 52.95 27.26 26.58 15.00
94PRO C 53.61 24.06 27.92 15.00
94PRO O 54.61 24.57 28.43 15.00
95GLU N 53.33 22.78 28.07 15.00
95GLU CA 54.25 21.92 28.80 15.00
95GLU CB 53.53 20.64 29.23 15.00
95GLU CG 52.19 20.88 29.95 15.00
95GLU CD 52.28 21.71 31.25 15.00
95GLU OE1 51.22 22.21 31.70 15.00
95GLU OE2 53.39 21.86 31.83 15.00
95GLU C 55.46 21.61 27.91 15.00
95GLU O 55.30 21.42 26.70 15.00
96TRP N 56.66 21.58 28.49 15.00
96TRP CA 57.88 21.32 27.71 15.00
96TRP CB 59.09 21.98 28.38 15.00
96TRP CG 59.20 21.70 29.82 15.00
96TRP CD2 59.29 22.67 30.87 15.00
96TRP CE2 59.44 21.96 32.09 15.00
96TRP CE3 59.26 24.07 30.89 15.00
96TRP CD1 59.29 20.48 30.43 15.00
96TRP NE1 59.44 20.62 31.79 15.00
96TRP CZ2 59.56 22.61 33.32 15.00
96TRP CZ3 59.38 24.71 32.12 15.00
41
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
96 TRP CH2 59.53 23.98 33.31 15.00
96 TRP C 58.18 19.86 27.43 15.00
96 TRP O 57.89 19.00 .28.24 15:00
97 GLU N 58.87 19.64 26.30 15.00
97 GLU CA 59.28 18.33 25.77 15.00
97 GLU CB 60.24 18.53 24.59 15.00
97 GLU CG 59.86 19.64 23.61 15.00
97 GLU CD 61.09 20.40 23.09 15.00
97 GLU OE1 61.77 19.91 22.15 15.00
97 GLU OE2 61.36 21.49 23.64 15.00
97 GLU C 59.89 17.31 26.75 15.00
97 GLU O 61.02 16.83 26.56 15.00
98 GLY N 59.08 16.92 27.73 15.00
98 GLY CA 59.49 15.97 28.75 15.00
98 GLY C 58.65 14.71 28.66 15.00
98 GLY O 58.23 14.30 27.58 15.00
99 ARG N 58.27 14.18 29.81 15.00
99 ARG CA 57.54 12.92 .29.84 15.00
99 ARG CB 58.60 11.81 29.82 15.00
99 ARG CG 58.16 10.40 29.50 15.00
99 ARG CD 59.36 9.55 29.09 15.00
99 ARG NE 59.78 9.84 27.71 15.00
99 ARG CZ 60.90 10.48 27.36 15.00
99 ARG NH1 61.75 10.93 28.28 15.00
99 ARG NH2 61.16 10.69 26.07 15.00
99 ARG C 56.79 12.95 31.18 15.00
99 ARG O 57.15 13.75 32.04 15.00
100 ALA N 55.77 12.11 31.36 15.00
100 ALA CA 55.04 12.13 32.64 15.00
100 ALA CB 53.72 12.88 32.49 15.00
100 ALA C 54.81 10.77 33.28 15.00
100 ALA O 54.45 9.79 32.61 15.00
101 PRO N 54.99 10.69 34.61 15.00
101 PRO CD 55.38 11.79 35.49 15.00
101 PRO CA 54.80 9.46 35.38 15.00
101 PRO CB 55.16 9.89 36.80 15.00
101 PRO CG 54.85 11.34 36.81 15.00
42
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
101PRO C 53.40 8.87 35.31 15.00
101PRO 0 52.44 9.56 34.97 15.00
102ASP N 53.30 7.59 35.64 15.00
102ASP CA 52.03 6.86 35.66 15.00
102ASP CB 52.30 5.36 35.82 15.00
102ASP CG 52.72 4.70 34.51 15.00
102ASP OD1 53.12 5.39 33.54 15.00
102ASP OD2 52.63 3.45 '34.45 15.00
102ASP C 51.16 7.36 36.83 15.00
102ASP O 50.09 7.92 36.62 15.00
103SER N 51.62 7.10 38.05 15.00
103SER CA 50.93 7.51 39.27 15.00
103SER CB 51.43 6.64 40.44 15.00
103SER OG 50.89 7.02 41.70 15.00
103SER C 51.28 8.99 39.51 15.00
103SER O 51.87 9.62 38.63 15.00
104VAL N 50.86 9.54 40.66 15.00
104VAL CA 51.13 10.93 41.06 15.00
104VAL CB 51.10 11.88 39.85 15.00
104VAL CG1 49.67 12.03 39.37 15.00
104VAL CG2 51.76 13.20 40.18 15.00
104VAL C 50.14 11.47 42.12 15.00
104VAL O 49.77 12.63 42.11 15.00
105ASP N 49.76 10.64 43.06 15.00
105ASP CA 48.82 11.06 44.09 15.00
105ASP CB 48.39 9.82 44.87 15.00
105ASP CG 47.34 10.10 45.93 15.00
105ASP OD1 47.33 11.19 46.55 15.00
105ASP OD2 46.53 9.18 46.17 15.00
105ASP C 49.54 12.05 44.99 15.00
105ASP O 50.36 11.64 45.82 15.00
106TYR N 49.23 13.34 44.89 15.00
106TYR CA 49.88 14.34 45.73 15.00
106TYR CB 49.55 15.74 45.28 15.00
106TY1~CG 50.43 16.20 44.17 25.00
106TYR CD1 51.75 16.55 44.42 15.00
106TYR CE1 52.58 16.95 43.38 15.00
43
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
106TYR CD2 49.96 16.25 42.87 15.00
106TYR CE2 50.78 16.65 41.83 15.00
106TYR CZ 52.09 16.99 42.09 15.00
106TYR OH 52.89 17.37 41.04 15.00
106TYR C 49.66 14.23 47.23 15.00
106TYR O 50.35 14.88 48.03 15.00
107ARG N 48.66 13.45 47.63 15.00
107ARG CA 48.40 13.29 49.05 15.00
107ARG CB 47.01 12.70 49.26 15.00
107ARG CG 45.92 13.66 48.83 15.00
107ARG CD 44.62 12.97 48.69 15.00
107ARG NE 44.58 12.10 .47.52 15.00
107ARG CZ 43.98 10.92 47.51 15.00
107ARG NH1 43.38 10.47 48.61 15.00
107ARG NH2 43.90 10.22 46.38 15.00
207ARG C 49.50 12.42 49.66 15.00
107ARG O 49.93 12.65 50.79 15.00
108LYS N 49.99 11.46 48.88 15.00
108LYS CA 51.09 10.60 49.32 15.00
108LYS CB 51.48 9.59 48.24 15.00
108LYS CG 50.50 8.45 48.02 15.00
108LYS CD 50.82 7.25 48.91 15.00
108LYS CE 49.90 6.05 48.60 15.00
108LYS NZ 49.85 5.66 47.15 15.00
108LYS C 52.26 11.56 49.53 15.00
108LYS O 52.99 11.46 50.51 15.00
109LYS N 52.37 12.52 48.61 15.00
109LYS CA 53.43 13.53 48.62 15.00
109LYS CB 53.61 14.12 47.22 15.00
109LYS CG 53.60 13.06 46.12 15.00
109LYS CD 53.94 13.64 44.75 15.00
109LYS CE 55.40 14.03 44.66 15.00
109LYS NZ 56.29 12.84 44.80 15.00
109LYS C 53.17 14.65 49.63 15.00
109LYS 0 53.76 15.72 49.54 15.00
110GLY N 52.23 14.41 50.54 15.00
110GLY CA 51.88 15.35 51.59 15.00
44
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
110GLY C 51.79 16.81 51.26 15.00
110GLY O 52.16 17.64 52.07 15.00
111TYR N 51.28 17.15 50.08 15.00
111TYR CA 51.13 18.55 49.66 15.00
111TYR CB 51.43 18.71 48.15 15.00
111TYR CG 52.89 18.57 47.73 15.00
111TYR CD1 53.87 18.20 48.65 15.00
111TYR CE1 55.20 18.05 48.27 15.00
111TYR CD2 53.29 18.80 46.41 15.00
111TYR CE2 54.62 18.66 46.02 15.00
111TYR CZ 55.57 18.27 46.97 15.00
111TYR OH 56.89 18.08 46.63 15.00
111TYR C 49.73 19.14 49.94 15.00
111TYR O 49.54 20.36 49.92 15.00
112VAL N 48.77 18.28 50.26 15.00
112VAL CA 47.38 18.71 .50.45 15.00
112VAL CB 46.46 17.87 49.56 15.00
112VAL CG1 45.13 18.59 49.37 15.00
112VAL CG2 47.13 17.52 48.23 15.00
112VAL C 46.81 18.70 51.87 15.00
112VAL O 46.97 17.73 52.63 15.00
113THR N 46.10 19.78 52.20 15.00
113THR CA 45.47 19.93 53.52 15.00
113THR CB 45.05 21.38 53.76 15.00
113THR OG1 44.71 22.00 52.52 15.00
113THR CG2 46.15 22.14 54.46 15.00
113THR C 44.20 19.09 53.59 15.00
113THR O 43.77 18.50 52.60 15.00
114PRO N 43.60 19.01 54.79 15.00
114PRO CD 44.13 19.46 56.09 15.00
114PRO CA 42.37 18.25 .54.99 15.00
114PRO CB 42.09 18.45 56.47 15.00
114PRO CG 43.42 18.55 57.06 15.00
114PRO C 41.24 18.82 54.13 15.00
114PRO O 41.36 19.94 53.62 15.00
115VAL N 40.16 18.05 53.97 15.00
115VAL CA 39.00 18.50 53.19 15.00
45
CA 02301201 2000-02-04
WO 00109653 PCT/US98/14634
115 VAL CB 38.13 17.31 52.69 15.00
115 VAL CG1 36.92 17.83 51.93 15.00
115 VAL CG2 38.93 16.41 51.77 15.00
115 VAL C 38.18 19.38 54.11 15.00
115 VAL O 37.77 18.97 55.19 15.00
116 LYS N 38.01 20.63 53.70 15.00
116 LYS CA 37.24 21.56 54.49 15.00
116 LYS CB 37.79 22.98 54.34 15.00
116 LYS CG 39.28 23.07 54.08 15.00
116 LYS CD 39.54 23.07 52.58 15.00
116 LYS CE 41.03 23.20 52.27 15.00
116 LYS NZ 41.56 24.48 52.82 15.00
116 LYS C 35.81 21.46 53.97 15.00
216 LYS O 35.55 20.71 53.03 15.00
117 ASN N 34.89 22.21 54.57 15.00
117 ASN CA 33.50 22.18 54.13 15.00
117 ASN CB 32.62 21.57 55.21 15.00
117 ASN CG 31.16 21.70 54.88 15.00
117 ASN OD1 30.61 20.93 54.10 15.00
117 ASN ND2 30.52 22.71 55.46 15.00
117 ASN C 33.02 23.59 53.81 15.00
117 ASN O 32.92 24.43 54.70 15.00
118 GLN N 32.65 23.81 52.55 15.00
118 GLN CA 32.21 25.12 52.08 15.00
118 GLN CB 32.17 25.14 50.55 15.00
118 GLN CG 30.98 24.41 49.94 15.00
118 GLN CD 30.83 24.71 48.46 15.00
118 GLN OE1 31.38 24.01 47.61 15.00
118 GLN NE2 30.11 25.77 48.24 15.00
118 GLN C 30.86 25.59 52.59 15.00
118 GLN O 30.32 26.57 52.09 15.00
119 GLY N 30.32 24.91 53.58 15.00
119 GLY CA 29.02 25.31 54.10 15.00
119 GLY C 28.03 25.31 52.96 15.00
119 GLY O 28.21 24.61 51.97 15.00
120 GLN N 27.01 26.16 '53.06 15.00
120 GLN CA 25.99 26.23 52.04 15.00
46
CA 02301201 2000-02-04
WO 00/09653 PCT/US98I14634
120 GLN CB 24.60 25.99 52.65 15.00
120 GLN CG 24.28 26.85 53.89 15.00
120 GLN CD 25.10 26.50 55.13 15.00
120 GLN OE1 26.03 27.22 55.52 15.00
120 GLN NE2 24.75 25.38 55.76 15.00
120 GLN C 26.10 27.61 51.41 15.00
120 GLN O 25.12 28.34 52.29 15.00
121 CYS N 27.33 27.97 51.04 15.00
121 CYS CA 27.63 29.26 50.43 15.00
121 CYS C 28.38 29.01 49.13 15.00
121 CYS 0 28.91 27.93 -48.92 15.00
121 CYS CB 28.43 30.13 51.42 15.00
121 CYS SG 29.68 31.27 50.76 15.00
122 GLY N 28.41 29.98 48.24 15.00
122 GLY CA 29.08 29.76 46.98 15.00
122 GLY C 30.58 29.94 47.04 15.00
122 GLY O 31.13 30.69 46.24 15.00
123 SER N 31.24 29.25 47.96 15.00
123 SER CA 32.69 29.37 48.08 15.00
123 SER CB 33.10 29.19 49.53 15.00
123 SER OG 32.79 30.33 50.30 15.00
123 SER C 33.44 28.36 47.23 15.00
123 SER O 34.63 28.16 47.44 15.00
124 CYS N 32.79 27.73 46.27 15.00
124 CYS CA 33.49 26.74 45.45 15.00
124 CYS CB 32.55 26.04 44.46 15.00
124 CYS SG 32.47 26.70 42.78 15.00
124 CYS C 34.71 27.36 44.77 15.00
124 CYS O 35.80 26.79 44.79 15.00
125 TRP N 34.54 28.58 44.25 15.00
125 TRP CA 35.62 29.31 43.58 15.00
125 TRP CB 35.11 30.63 42.97 15.00
125 TRP CG 34.60 31.64 43.98 15.00
125 TRP CD2 35.30 32.77 44.49 15.00
125 TRP CE2 34.48 33.37 45.47 15.00
125 TRP CE3 36.56 33.34 44.23 15.00
125 TRP CD1 33.40 31.61 44.63 15.00
47
CA 02301201 2000-02-04
WO 00/09653 PCTIITS98/14634
125 TRP NE1 33.33 32.64 45.53 15.00
125 TRP CZ2 34.87 34.51 46.18 15.00
125 TRP CZ3 36.95 34.47 44.94 15.00
125 TRP CH2 36.10 35.04 45.90 15.00
125 TRP C 36.72 29.59 44.60 15.00
125 TRP O 37.89 29.34 44.34 15.00
126 ALA N 36.33 30.05 45.79 15.00
126 ALA CA 37.30 30.34 46.83 15.00
126 ALA CB 36.68 31.21 47.90 15.00
126 ALA C 37.90 29.09 47.45 15.00
126 ALA O 38.80 29.19 48.28 15.00
127 PHE N 37.39 27.92 47.12 15.00
127 PHE CA 37.98 26.72 47.69 15.00
127 PHE CB 36.92 25.73 48.16 15.00
127 PHE CG 36.48 25.95 49.59 15.00
127 PHE CD1 35.54 26.93 49.90 15.00
127 PHE CD2 37.03 25.21 50.62 15.00
127 PHE CE1 35.17 27.16 51.21 15.00
127 PHE CE2 36.66 25.45 51.95 15.00
127 PHE CZ 35.72 26.42 52.24 15.00
127 PHE C 38.98 26.09 46.74 15.00
127 PHE O 40.05 25.67 47.16 15.00
128 SER N 38.66 26.08 45.46 15.00
228 SER CA 39.56 25.54 44.45 15.00
128 SER CB 38.91 25.64 43.07 15.00
128 SER OG 39.60 24.88 42.09 15.00
128 SER C 40.85 26.35 44.50 15.00
128 SER O 42.95 25.80 44.63 15.00
129 SER N 40.72 27.68 44.44 15.00
129 SER CA 41.86 28.57 .44.47 15.00
129 SER CB 41.40 30.01 44.43 15.00
129 SER OG 40.65 30.23 43.25 15.00
129 SER C 42.69 28.33 45.69 15.00
129 SER O 43.87 28.02 45.59 15.00
130 VAL N 42.07 28.41 46.85 15.00
130 VAL CA 42.79 28.18 48.10 15.00
130 VAL CB 41.81 28.14 49.28 15.00
48
CA 02301201 2000-02-04
WO 00109653 PCT/US98/14634
130VAL CG1 42.27 27.18 50.35 15.00
130VAL CG2 41.70 29.52 49.85 15.00
130VAL C 43.58 26.88 48.04 15.00
130VAL O 44.65 26.79 48.62 15.00
131GLY N 43.08 25.91 47.29 15.00
131GLY CA 43.74 24.61 47.18 15.00
131GLY C 45.07 24.66 46.45 15.00
131GLY 0 46.06 24.12 46.94 15.00
132ALA N 45.07 25.26 45.27 15.00
132ALA CA 46.27 25.39 44.47 15.00
132ALA CB 45.92 26.00 43,15 15.00
132ALA C 47.30 26.25 45.21 15.00
132ALA O 48.51 26.01 45.13 15.00
133LEU N 46.82 27.26 45.93 15.00
133LEU CA 47.70 28.13 46.69 15.00
133LEU CB 46.92 29.27 47.32 15.00
133LEU CG 46.66 30.36 46.31 15.00
133LEU CD1 45.87 31.46 46.98 15.00
133LEU CD2 47.97 30.90 45.76 15.00
133LEU C 48.46 27.36 47.76 15.00
133LEU O 49.65 27.58 47.96 15.00
134GLU N 47.77 26.45 48.43 15.00
134GLU CA 48.40 25.64 49.46 15.00
134GLU CB 47.35 24.78 50.17 15.00
134GLU CG 46.35 25.63 50.99 15.00
134GLU CD 45.02 24.94 51.29 15.00
134GLU OE1 44.30 25.44 52.17 15.00
134GLU OE2 44.69 23.92 50.65 15.00
234GLU C 49.52 24.78 48.86 15.00
134GLU O 50.67 24.91 49.26 25.00
135GLY N 49.18 24.01 47.83 15.00
135GLY CA 50.13 23.13 47.15 15.00
135GLY C 51.43 23.81 46.79 15.00
135GLY O 52.51 23.19 46.84 15.00
136GLN N 51.33 25.08 46.41 15.00
136GLN CA 52.49 25.88 46.06 15.00
136GLN CB 52.04 27.14 45.34 15.00
49
CA 02301201 2000-02-04
WO 00/09653 PCTIUS98/14634
136 GLN CG 51.39 26.82 44.03 15.00
136 GLN CD 52.23 25.85 43.24 15.00
136 GLN OE1 52.02 24.64 43.33 15.00
136 GLN NE2 53.23 26.36 42.53 15.00
136 GLN C 53.23 26.24 47.34 15.00
136 GLN O 54.44 26.02 47.45 15.00
137 LEU N 52.50 26.74 48.34 15.00
137 LEU CA 53.10 27.10 49.62 15.00
137 LEU CB 52.02 27.35 50.67 15.00
137 LEU CG 52.49 27.78 52.05 15.00
137 LEU CD1 53.16 29.11 51.94 15.00
137 LEU CD2 51.32 27.87 52,99 15.00
137 LEU C 53.98 25.94 50.08 15.00
137 LEU O 55.10 26.14 50.55 15.00
138 LYS N 53.49 24.73 49.87 15.00
138 LYS CA 54.23 23.54 50.25 15.00
138 LYS CB 53.37 22.30 50.06 15.00
138 LYS CG 54.09 21.00 50.39 15.00
138 LYS CD 54.37 20.87 51.88 15.00
138 LYS CE 54.98 19.52 52.20 15.00
138 LYS NZ 55.22 19.34 53.66 15.00
138 LYS C 55.48 23.42 49.39 15.00
138 LYS O 56.60 23.42 49.90 15.00
139 LYS N 55.27 23.37 '48.08 15.00
139 LYS CA 56.35 23.22 47.12 15.00
139 LYS CB 55.76 22.98 45.72 15.00
139 LYS CG 56.78 22.68 44.61 15.00
139 LYS CD 56.11 22.53 43.25 15.00
139 LYS CE 57.10 22.11 42.18 15.00
139 LYS NZ 56.47 21.93 40.84 15.00
139 LYS C 57.36 24.36 47.10 15.00
139 LYS 0 58.36 24.27 46.40 15.00
140 LYS N 57.13 25.43 47.87 15.00
240 LYS CA 58.07 26.55 47.86 15.00
140 LYS CB 57.47 27.72 47.08 15.00
140 LYS CG 58.47 28.78 46.62 15.00
140 LYS CD 57.75 29.84 45.80 15.00
Sb
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
140 LYS CE 58.71 30.80 45.06 15.00
140 LYS NZ 59.20 30.29 43.72 15.00
140 LYS C 58.47 27.02 49.26 15.00
140 LYS O 59.15 28.04 49.40 15.00
141 THR N 58.06 26.28 50.28 15.00
141 THR CA 58.40 26.63 51.67 15.00
141 THR CB 57.41 27.66 52.27 15.00
141 THR OG1 57.48 28.88 51.52 15.00
141 THR CG2 57.73 27.97 53.72 15.00
141 THR C 58.51 25.38 52.55 15.00
141 THR O 58.92 25.45 53.71 15.00
142 GLY N 58.16 24.22 51.99 15.00
142 GLY CA 58.27 22.97 52.72 25.00
142 GLY C 57.25 22.78 53.83 15.00
142 GLY O 57.03 21.67 54.29 15.00
143 LYS N 56.72 23.89 54.33 15.00
143 LYS CA 55.69 23.84 55.34 15.00
143 LYS CB 55.76 25.09 56.22 15.00
143 LYS CG 56.85 25.06 57.26 15.00
143 LYS CD 56.33 24.57 58.61 15.00
143 LYS CE 55.35 25.58 59.22 15.00
143 LYS NZ 54.85 25.16 60.56 15.00
143 LYS C 54.37 23.83 54.58 15.00
143 LYS O 54.32 24.20 53.40 15.00
144 LEU N 53.31 23.39 55.23 15.00
144 LEU CA 52.00 23.37 54.60 15.00
144 LEU CB 51.70 21.99 54.02 15.00
144 LEU CG 50.28 21.43 54.19 15.00
.
144 LEU CD1 49.90 20.63 52.95 15.00
144 LEU CD2 50.19 20.58 55.46 15.00
144 LEU C 50.97 23.72 55.65 15.00
144 LEU O 50.97 23.14 56.74 15.00
145 LEU N 50.16 24.72 55.36 15.00
145 LEU CA 49.12 25.12 56.28 15.00
145 LEU CB 49.59 26.22 57.23 15.00
145 LEU CG 50.20 27.54 56.77 15.00
145 LEU CD1 50.04 28.57 57.88 15.00
51
CA 02301201 2000-02-04
WO 00/09653 PCTIUS98/14634
145 LEU CD2 51.68 27.36 56.43 15.00
145 LEU C 47.89 25.54 55.49 15.00
145 LEU O 47.95 25.73 '54.27 15.00
146 ASN N 46.77 25.64 56.20 15.00
146 ASN CA 45.51 26.02 55.57 15.00
146 ASN CB 44.33 25.53 56.41 15.00
146 ASN CG 44.65 24.27 57.19 15.00
146 ASN OD1 45.62 23.58 56.90 15.00
146 ASN ND2 43.84 23.99 58.20 15.00
146 ASN C 45.40 27.52 55.41 15.00
146 ASN O 45.53 28.27 56.39 15.00
147 LEU N 45.16 27.96 54.18 15.00
147 LEU CA 44.99 29.36 53.84 15.00
147 LEU CB 45.51 29.62 52.43 15,00
147 LEU CG 46.91 29.08 52.19 15.00
147 LEU CD1 47.34 29.38 50.77 15.00
147 LEU CD2 47.85 29.70 53.18 15.00
147 LEU C 43.49 29.62 53.91 15.00
147 LEU O 42.71 28.70 53.68 15.00
148 SER N 43.10 30.85 54.20 15.00
148 SER CA 41.68 31.21 54.31 15.00
148 SER CB 41.52 32.50 55.12 15.00
148 SER OG 40,20 32.98 55.01 15.00
148 SER C 40.90 31.36 53.00 15.00
148 SER O 41.20 32.22 52.18 15.00
149 PRO N 39.82 30.57 52.85 15.00
149 PRO CD 39.42 29.53 53.81 15.00
149 PRO CA 38.92 30.56 51.69 15.00
149 PRO CB 38.09 29.30 51.91 15.00
149 PRO CG 38.90 28.48 52.90 15.00
149 PRO C 38.04 31.$0 51.73 15.00
149 PRO O 37.68 32.37 50.70 15.00
150 GLN N 37.72 32.23 52.94 15.00
150 GLN CA 36.88 33.41 53.09 15.00
150 GLN CB 36.42 33.61 54.53 25.00
150 GLN CG 35.40 34.72 54.62 15.00
150 GLN CD 34.35 34.51 55.69 15.00
52
CA 02301201 2000-02-04
WO 00109653 PCT/US98/14634
150GLN OE1 33.83 33.41 55.87 15.00
150GLN NE2 34.01 35.59 56.37 15.00
150GLN C 37.59 34.65 52.55 15.00
150GLN O 37.05 35.31 51.67 15.00
151ASN N 38.79 34.94 53.06 15.00
151ASN CA 39.60 36.10 52.65 15.00
151ASN CB 41.10 35.74 -52.72 15.00
251ASN CG 42.01 36.96 52.57 15.00
151ASN OD1 42.13 37.55 51.50 15.00
151ASN ND2 42.69 37.31 53,65 15.00
151ASN C 39.26 36.44 51.22 15.00
151ASN O 38.89 37.57 50.91 15.00
152LEU N 39.30 35.42 50.38 15.00
152LEU CA 38.97 35.57 48.99 15.00
152LEU CB 39.13 34.25 48.23 15.00
152LEU CG 40.51 33.63 48.09 15.00
152LEU CD1 41.50 34.67 47.60 15.00
152LEU CD2 40.95 33.07 49.41 15.00
152LEU C 37.53 36.04 48.93 15.00
152LEU O 37.29 37.20 48.60 15.00
153VAL N 36.59 35.19 49.34 15.00
153VAL CA 35.16 35.50 49.32 15.00
153VAL CB 34.35 34.61 50.31 15.00
153VAL CG1 32.85 34.96 50.23 15.00
153VAL CG2 34.56 33.13 50.03 15.00
153VAL C 34.78 36.95 49.62 15.00
153VAL O 34.07 37.57 48.82 15.00
154ASP N 35.21 37.46 50.77 15.00
154ASP CA 34.89 38.84 51.17 15.00
154ASP CB 35.15 39.06 52.67 15.00
154ASP CG 34.67 37.94 53.54 15.00
154ASP OD1 34.06 36.98 53.01 15.00
154ASP OD2 34.91 38.00 54.77 15.00
154ASP C 35.68 39.91 50.44 15.00
154AS1~O 35.16 41.00 50.20 15.00
155CYS N 36.95 39.64 50.15 15.00
155CYS CA 37.78 40.64 49.51 15.00
53
CA 02301201 2000-02-04
WO 00109653 PCT/US98/14634
155 CYS C 37.79 40.73 47.99 15.00
155 CYS O 37.81 41.83 47.45 15.00
155 CYS CB 39.17 40.56 50.08 15.00
155 CYS SG 39.15 40.52 51.90 15.00
156 VAL N 37.82 39.59 47.30 15.00
156 VAL CA 37.83 39.60 45.84 25.00
156 VAL CB 37.88 38.17 45.26 15.00
156 VAL CG1 37.89 38.22 43.75 15.00
156 VAL CG2 39.11 37.48 45.75 15.00
156 VAL C 36.59 40.33 45.30 15.00
156 VAL O 35.64 39.70 44.83 15.00
157 SER N 36.64 41.65 45.31 15.00
157 SER CA 35.53 42.48 44.85 15.00
157 SER CB 35.92 43.96 44.90 15.00
157 SER OG 37.07 44.17 45.70 15.00
157 SER C 35.10 42.14 43.45 15.00
157 SER 0 34.07 42.63 42.99 15.00
158 GLU N 35.90 41.36 42.74 15.00
158 GLU CA 35.56 40.99 41.37 15.00
158 GLU CB 36.83 40.69 40.56 15.00
158 GLU CG 37.78 41.88 40.35 15.00
158 GLU CD 39.15 41.67 41.00 15.00
158 GLU OE1 39.62 40.51 41.07 15.00
158 GLU OE2 39.76 42.68 41.46 15.00
158 GLU C 34.63 39.77 41.34 15.00
158 GLU O 34.06 39.43 .40.30 15.00
159 ASN N 34.46 39.12 42.49 15.00
159 ASN CA 33.62 37.93 42.57 15.00
159 ASN CB 34.47 36.70 42.94 15.00
159 ASN CG 35.27 36.15 41.75 15.00
159 ASN OD1 36.23 35.41 41.95 15.00
159 ASN ND2 34.85 36.48 40.53 15.00
159 ASN C 32.45 38.09 43.53 15.00
159 ASN O 32.57 38.70 44.59 15.00
160 ASP N 31.32 37.49 43.17 15.00
160 ASP CA 30.10 37.60 43.97 15.00
160 ASP CB 28.91 37.01 43.19 15.00
54
CA 02301201 2000-02-04
WO 00109653 PCT/IJS98/14634
160ASP CG 27.72 37.96 43.13 15.00
160ASP OD1 27.04 37.99 42.07 15.00
160ASP OD2 27.47 38.68 44.12 15.00
160ASP C 30.16 36.96 45.35 15.00
160ASP 0 29.12 36.80 46.00 15.00
161GLY N 31.35 36.59 45.80 15.00
161GLY CA 31.46 35.98 47.11 15.00
161GLY C 30.58 34.75 47.20 15.00
161GLY O 30.57 33.93 46.28 15.00
162CYS N 29.77 34.67 48.25 15.00
162CYS CA 28.88 33.53 48.42 15.00
162CYS C 27.91 33.35 47.27 15.00
162CYS O 27.27 32.31 47.16 15.00
162CYS CB 28.11 33.63 49.74 15.00
162CYS SG 29.06 33.14 51.22 15.00
163GLY N 27.80 34.35 '46.40 15.00
163GLY CA 26.89 34.24 45.29 15.00
163GLY C 27.55 33.80 44.00 15.00
163GLY O 26.94 33.84 42.93 15.00
164GLY N 28.81 33.41 44.09 15.00
164GLY CA 29.51 32.96 42.90 15.00
164GLY C 30.80 33.70 42.64 15.00
164GLY O 31.08 34.75 43.23 15.00
165GLY N 31.60 33.14 41.73 15.00
165GLY CA 32.87 33.73 41.37 15.00
165GLY C 33.69 32.80 40.50 15.00
165GLY O 33.15 31.84 39.94 15.00
166TYR N 34.99 33.03 40.42 15.00
166TYR CA 35.87 32.22 39.60 15.00
166TYR CB 35.91 32.77 38.19 15.00
166TYR CG 36.63 31.87 '37.25 15.00
166TYR CD1 37.97 32.08 36.95 15.00
166TYR CE1 38.64 31.27 36.05 15.00
166TYR CD2 35.98 30.82 36.64 15.00
166TYR CE2 36.64 30.00 35.73 15.00
166TYR CZ 37.96 30.23 35.43 15.00
166TYR OH 38.59 29.45 34.50 15.00
55
CA 02301201 2000-02-04
WO 00109653 PCT/US98/14634
166 TYR C 37.28 32.18 40.15 15.00
166 TYR O 37.67 33.05 40.93 15.00
167 MET N 38.08 31.24 39.66 15.00
167 MET CA 39.44 31.09 40.16 15.00
167 MET CB 39.93 29.66 .39.91 15.00
167 MET CG 39.21 28.59 40.73 15.00
167 MET SD 37.76 27.96 39.92 15.00
167 MET CE 38.38 27.66 38.30 15.00
167 MET C 40.51 32.11 39.77 15.00
167 MET 0 41.15 32.69 40.66 15.00
168 THR N 40.72 32.34 38.48 15.00
168 THR CA 41.73 33.30 38.05 15.00
168 THR CB 41.79 33.47 36.51 15.00
168 THR OG1 40.50 33.82 36.01 15.00
168 THR CG2 42.21 32.18 35.84 15.00
168 THR C 41.59 34.64 38.79 15.00
168 THR O 42.57 35.17 39.31 15.00
169 ASN N 40.36 35.14 38.92 15.00
169 ASN CA 40.14 36.39 39.64 15.00
169 ASN CB 38.66 36.76 39.66 15.00
169 ASN CG 38.27 37.62 38.50 15.00
169 ASN OD1 37.59 37.17 37.58 15.00
169 ASN ND2 38.70 38.87 38.52 15.00
169 ASN C 40.64 36.31 41.07 15.00
169 ASN O 40.97 37.33 41.65 15.00
170 ALA N 40.67 35.11 41.64 15.00
170 ALA CA 41.14 34.94 43.01 15.00
170 ALA CB 40.66 33.62 43.57 15.00
170 ALA C 42.67 35.02 43.12 15.00
170 ALA O 43.22 35.37 44.17 15.00
171 PHE N 43.34 34.63 42.05 15.00
171 PHE CA 44.79 34.64 42.03 15.00
171 PHE CB 45.31 33.72 40.93 15.00
171 PHE CG 45.00 32.26 41.18 15.00
171 PHE CD1 45.01 31.75 42.46 15.00
171 PHE CD2 44.71 31.41 40.12 15.00
171 PHE CE1 44.73 30.41 42.70 15.00
56
CA 02301201 2000-02-04
WO 00/09653 PCT/US98114634
171 PHE CE2 44.44 30.06 40.35 15.00
171 PHE CZ 44.45 29.57 41.65 15.00
171 PHE C 45.31 36.06 41.88 15.00
171 PHE O 46.16 36.49 42.68 15.00
172 GLN N 44.77 36.82 40.93 15.00
172 GLN CA 45.21 38.20 40.76 15.00
172 GLN CB 44.56 38.85 39.53 15.00
172 GLN CG 43.15 39.38 39.76 15.00
172 GLN CD 42.50 39.90 38.48 15.00
172 GLN OE1 42.71 39.36 37.38 15.00
172 GLN NE2 41.69 40.95 38.61 15.00
172 GLN C 44.95 39.05 42.02 15.00
172 GLN O 45.63 40.04 42.26 15.00
173 TYR N 43.97 38.65 42.83 15.00
173 TYR CA 43.67 39.38 44.05 15.00
173 TYR CB 42.40 38.87 44.74 15.00
173 TYR CG 42.31 39.27 46.21 15.00
173 TYR CD1 42.08 40.59 46.59 15.00
173 TYR CE1 42.08 40.96 47.94 15.00
173 TYR CD2 42.54 38.33 47.21 15.00
173 TYR CE2 42.55 38.69 4 8.56 15.00
173 TYR CZ 42.32 40.00 48.91 15.00
173 TYR OH 42.35 40.33 50.24 15.00
173 TYR C 44.83 39.26 .45.03 15.00
173 TYR O 45.30 40.26 45.56 15.00
174 VAL N 45.29 38.04 45.27 15.00
174 VAL CA 46.39 37.82 46.20 15.00
174 VAL CB 46.76 36.32 46.27 15.00
174 VAL CG1 47.52 36.03 47.56 15.00
174 VAL CG2 45.51 35.45 46.17 15.00
174 VAL C 47.62 38.62 45.75 15.00
174 VAL O 48.20 39.38 46.54 15.00
175 GLN N 47.98 38.48 44.48 15.00
175 GLN CA 49.12 39.18 43.88 15.00
175 GLN CB 49.09 38.97 42.36 15.00
175 GLN CG 49.97 39.89 41.54 15.00
175 GLN CD 49.72 39.72 40.06 15.00
57
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
175GLN OE1 49.68 38.60 39.56 15.00
175GLN NE2 49.51 40.82 39.36 15.00
175GLN C 49.08 40.67 44.19 15.00
175GLN O 49.89 41.18 44.98 15.00
176LYS N 48.09 41.36 43.63 15.00
176LYS CA 47.95 42.79 43.$3 15.00
176LYS CB 46.79 43.32 42.99 15.00
176LYS CG 47.01 43.12 41.48 15.00
176LYS CD 45.77 43.45 40.65 15.00
176LYS CE 45.42 44.92 40.74 15.00
176LYS NZ 46.54 45.80 40.27 15.00
176LYS C 47.76 43.14 45.30 15.00
176LYS O 48.07 44.26 45.72 15.00
177ASN N 47.33 42.17 46.09 15.00
177ASN CA 47.09 42.36 47.52 15.00
177ASN CB 45.87 41.52 47.95 15.00
177ASN CG 45.72 41.43 49.47 15.00
177ASN OD1 45.26 42.37 50.13 15.00
177ASN ND2 46.12 40.29 50.02 15.00
177ASN C 48.30 42.05 48.41 15.00
177ASN O 48.28 42.38 49.60 15.00
178ARG N 49.34 41.43 47.86 15.00
178ARG CA 50.56 41.07 48.61 15.00
178ARG CB 51.19 42.27 49.35 15.00
178ARG CG 51.37 43.55 48.57 15.00
178ARG CD 52.20 43.37 47.33 15.00
178ARG NE 52.30 44.62 46.60 15.00
178ARG CZ 52.31 44.71 45.28 15.00
178ARG NH1 52.22 43.61 _44.53 15.00
178ARG NH2 52.48 45.89 44.70 15.00
178ARG C 50.34 39.99 49.65 15.00
178ARG 0 50.95 40.04 50.71 15.00
179GLY N 49.45 39.04 49.38 15.00
179GLY CA 49.24 37.97 50.34 15.00
179GLY C 47.84 37.46 50.53 15.00
179GLY O 46.89 37.97 49.95 15.00
180ILE N 47.73 36.41 51.33 15.00
58
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
180 ILE CA 46.46 35.77 51.64 15.00
180 ILE CB 46.17 34.60 50.69 15.00
180 ILE CG2 47.30 33.58 50.74 15.00
180 ILE CG1 44.82 33.97 51.03 15.00
180 ILE CD1 44.48 32.76 50.21 15.00
180 ILE C 46.57 35.27 53.06 15.00
180 ILE O 47.49 34.54 53.41 15.00
181 ASP N 45.64 35.72 53.90 15.00
181 ASP CA 45.60 35.33 55.30 15.00
181 ASP CB 44.50 36.12 56.00 15.00
181 ASP CG 45.00 36.86 57.22 15.00
181 ASP OD1 44.78 38.09 57.30 15.00
181 ASP OD2 45.60 36.21 58.10 15.00
181 ASP C 45.32 33.84 55.39 15.00
181 ASP O 44.86 33.23 54.43 15.00
182 SER N 45.60 33.24 56.53 15.00
182 SER CA 45.36 31.82 56.66 15.00
182 SER CB 46.51 31.16 57.42 15.00
182 SER OG 46.40 32.38 .58.81 15.00
182 SER C 44.07 31.58 57.40 15.00
182 SER O 43.68 32.37 58.25 15.00
183 GLU N 43.43 30.45 57.12 15.00
183 GLU CA 42.19 30.07 57.79 15.00
183 GLU CB 41.86 28.61 57.44 15.00
183 GLU CG 40.75 27.96 58.27 15.00
183 GLU CD 39.39 28.59 58.04 15.00
183 GLU OE1 39.09 29.02 56.90 15.00
183 GLU OE2 38.61 28.63 59.01 15.00
183 GLU C 42.42 30.21 59.28 15.00
183 GLU O 41.61 30.74 60.02 15.00
184 ASP N 43.62 29.82 59.69 15.00
184 ASP CA 44.01 29.89 61.09 15.00
184 ASP CB 45.46 29.38 61.25 15.00
'
184 ASP CG 45.79 28.22 60.31 15.00
184 ASP OD1 45.54 27.05 60.71 15.00
184 ASP OD2 46.27 28.47 59.18 15.00
184 ASP C 43.90 31.32 61.60 15.00
59
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
184ASP O 43.51 31.54 62.74 15.00
185ALA N 44.22 32.28 60.74 15.00
185ALA CA 44.19 33.69 61.12 15.00
185ALA CB 45.50 34.36 60.72 15.00
185ALA C 43.02 34.48 60.56 15.00
185ALA O 42.92 35.69 60.78 15.00
186TYR N 42.11 33.81 59.88 15.00
186TYR CA 40.98 34.49 59.26 15.00
186TYR CB 41.49 35.24 58.02 15.00
186TYR CG 40.53 36.17 57.35 15.00
186TYR CD1 39.34 36.56 57.97 15.00
186TYR CE1 38.48 37.45 57.34 15.00
186TYR CD2 40.82 36.69 56.10 15.00
186TYR CE2 39.96 37.58 55.47 15.00
186TYR CZ 38.80 37.95 56.10 15.00
186TYR OH 37.97 38.84 55.47 15.00
186TYR C 40.02 33.39 58.86 25.00
186TYR O 40.00 32.96 57.71 15.00
187PRO N 39.24 32.89 59.82 15.00
187PRO CD 39.28 33.44 61.19 15.00
187PRO CA 38.23 31.83 59.73 15.00
187PR0 CB 37.55 31.90 61.09 15.00
187PRO CG 38.65 32.33 61.99 15.00
187PRO C 37.20 31.93 .58.62 15.00
187PRO O 36.96 32.99 58.07 15.00
188TYR N 36.56 30.81 58.36 15.00
188TYR CA 35.52 30.72 57.34 15.00
188TYR CB 35.65 29.41 56.55 15.00
188TYR CG 34.66 29.26 55.41 15.00
188TYR CD1 34.74 30.08 54.27 15.00
188TYR CE1 33.85 29.92 53.20 15.00
188TYR CD2 33.66 28.28 55.45 15.00
188TYR CE2 32.77 28.12 54.38 15.00
188TYR CZ 32.88 28.94 53.26 15.00
188TYR OH 32.03 28.77 52.21 15.00
188TYR C 34.23 30.68 58.14 15.00
188TYR O 34.13 29.94 59.12 15.00
60
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
189VAL N 33.26 31.49 57.76 15.00
189VAL CA 32.00 31.48 58.50 15.00
189VAL CB 31.63 32.87 59.03 15.00
189VAL CG1 30.21 32.86 59.62 15.00
189VAL CG2 32.65 33.28 60.11 15.00
189VAL C 30.87 30.90 57.68 15.00
189VAL O 30.04 30.16 58.19 15.00
190GLY N 30.85 31.21 56.39 15.00
190GLY CA 29.81 30.71 55.52 15.00
190GLY C 29.09 31.87 54.89 15.00
190GLY O 28.83 31.90 53.69 15.00
191GLN N 28.81 32.88 55.70 15.00
191GLN CA 28.11 34.05 .55.21 15.00
191GLN CB 27.13 34.55 56.28 15.00
191GLN CG 27.81 34.96 57.59 15.00
191GLN CD 25.86 34.98 58.76 15.00
191GLN OE1 26.34 33.93 59.18 15.00
191GLN NE2 26.61 36.17 59.31 15.00
191GLN C 29.12 35.13 54.90 15.00
191GLN O 29.97 35.43 55.73 15.00
192GLU N 29.04 35.71 53.71 15.00
192GLU CA 29.97 36.78 53.34 15.00
192GLU CB 29.72 37.25 51.90 15.00
192GLU CG 28.31 37.78 51.63 15.00
192GLU CD 28.07 38.20 50.17 15.00
192GLU OE1 27.23 39.09 49.94 15.00
192GLU OE2 28.71 37.63 49.24 15.00
192GLU C 29.84 37.94 54.33 15.00
192GLU O 28.83 38.06 55.02 15.00
193GLU N 30.90 38.73 54.45 15.00
193GLU CA 30.90 39.90 55.33 15.00
193GLU CB 31.14 39.51 56.79 15.00
193GLU CG 32.18 38.42 57.04 15.00
193GLU CD 32.27 38.04 58.52 15.00
193GLU OE1 33.25 37.35 58.91 15.00
193GLU OE2 31.38 38.45 59.31 15.00
193GLU C 31.93 40.91 54.83 15.00
61
CA 02301201 2000-02-04
WO 00109653 PCT/US98/14634
193GLU O 32.49 40.73 53.75 15.00
194SER N 32.15 41.98 55.58 15.00
194SER CA 33.12 42.98 55.15 15.00
194SER CB 33.02 44.25 56.02 15.00
194SER OG 31.89 45.03 55.66 15.00
194SER C 34.56 42.47 55.13 15.00
194SER 0 35.04 41.86 56.09 15.00
195CYS N 35.24 42.70 54.00 15.00
195CYS CA 36.63 42.28 53.84 15.00
195CYS C 37.45 42.85 54.99 15.00
195CYS O 37.66 44.06 55.08 15.00
195CYS CB 37.21 42.77 52.52 15.00
195CYS SG 39.00 42.47 52.43 15.00
196MET N 37.88 41.97 55.87 15.00
196MET CA 38.66 42.37 57.02 15.00
196MET CB 37.88 42.07 58.30 15.00
196MET CG 37.23 40.70 58.31 15.00
196MET SD 36.08 40.54 ,59.66 15.00
196MET CE 37.05 39.49 60.82 15.00
196MET C 39.98 41.63 56.99 15.00
196MET O 40.27 40.79 57.85 15.00
197TYR N 40.75 41.92 55.95 15.00
197TYR CA 42.06 41.33 55.74 15.00
197TYR CB 42.54 41.61 54.32 15.00
197TYR CG 43.92 41.10 54.00 15.00
197TYR CD1 44.75 41.78 53.12 15.00
197TYR CE2 45.98 41.28 52.76 15.00
197TYR CD2 44.38 39.89 54.52 15.00
197TYR CE2 45.62 39.38 54.16 15.00
197TYR CZ 46.40 40.08 53.28 15.00
197TYR OH 47.57 39.52 52.82 15.00
197TYR C 43.02 41.98 56.69 15.00
197TYR O 42.85 43.14 .57.03 15.00
198ASN N 44.04 41.24 57.10 15.00
198ASN CA 45.06 41.78 57.98 15.00
198ASN CB 44.76 41.50 59.44 15.00
198ASN CG 45.80 42.08 60.36 15.00
62
CA 02301201 2000-02-04
WO 00/09653 PCT/US98114634
198ASN ODl 46.82 42.59 59.89 15.00
198ASN ND2 45.55 42.04 61.66 15.00
198ASN C 46.41 41.20 57.63 15.00
198ASN O 46.68 40.02 57.89 15.00
199PRO N 47.31 42.02 57.10 15.00
199PRO CD 47.07 43.46 56.88 15.00
199PRO CA 48.66 41.66 56.70 15.00
199PRO CB 49.28 43.01 -56.34 15.00
199PRO CG 48.10 43.80 55.84 15.00
199PRO C 49.49 40.91 57.76 15.00
199PRO 0 50.45 40.22 57.43 15.00
200THR N 49.10 41.04 59.02 15.00
200THR CA 49.82 40.36 60.09 15.00
200THR CB 49.29 40.80 61.44 15.00
200THR OG1 47.92 40.37 61.56 15.00
200THR CG2 49.37 42.33 61.58 15.00
200THR C 49.70 38.83 60.00 15.00
200THR O 50.46 38.09 60.62 15.00
201GLY N 48.68 38.38 59.27 15.00
201GLY CA 48.47 36.96 59.10 15.00
201GLY C 48.75 36.56 57.66 15.00
201GLY 0 48.15 35.63 57.15 15.00
202LYS N 49.61 37.33 56.99 15.00
202LYS CA 49.98 37.05 55.61 15.00
202LYS CB 51.10 38.02 55.17 25.00
202LYS CG 51.29 38.17 53.66 15.00
202LYS CD 52.46 39.10 53.34 15.00
202LYS CE 52.21 40.54 53.79 15.00
202LYS NZ 51.96 41.48 52.65 15.00
202LYS C 50.52 35.64 55.69 15.00
202LYS O 51.33 35.33 56.57 15.00
203ALA N 50.08 34.77 54.79 15.00
203ALA CA 50.52 33.38 54.86 15.00
203ALA CB 49.35 32.49 55.25 15.00
203ALA C 51.16 32.87 53.60 15.00
203ALA O 52.05 32.03 53.65 15.00
204ALA N 50.68 33.33 52.46 15.00
63
CA 02301201 2000-02-04
WO 00109653 PCTIUS98/14634
204ALA CA 51.23 32.90 51.20 15.00
204ALA CB 50.40 31.76 50.65 15.00
204ALA C 51.17 34.09 50.27 15.00
204ALA 0 50.71 35.16 50.66 15.00
205LYS N 51.65 33.91 49.05 15.00
205LYS CA 51.64 34.98 48.07 15.00
205LYS CB 52.98 35.71 48.04 15.00
205LYS CG 53.06 36.98 48.87 15.00
205LYS CD 54.37 37.70 48.59 15.00
205LYS CE 54.47 38.99 49.39 15.00
205LYS NZ 55.68 39.79 49.04 15.00
205LYS C 51.38 34.38 .46.71 15.00
205LYS 0 51.31 33.18 46.55 15.00
206CYS N 51.29 35.24 45.72 15.00
206CYS CA 51.05 34.77 44.38 15.00
206CYS CB 49.56 34.72 44.11 15.00
206CYS SG 49.22 34.26 42.42 15.00
206CYS C 51.68 35.81 43.53 15.00
206CYS O 51.64 36.98 43.86 15.00
207ARG N 52.31 35.37 42.45 15.00
207ARG CA 52.97 36.30 41.56 15.00
207ARG CB 54.49 36.03 41.61 15.00
207ARG CG 55.05 36.33 43.04 15.00
207ARG CD 56.51 35.94 43.35 15.00
207ARG NE 56.81 36.25 44.76 15.00
207ARG CZ 57.91 36.88 45.20 15.00
207ARG NH1 58.87 37.28 44.36 15.00
207ARG NH2 58.01 37.20 46.49 15.00
207ARG C 52.37 36.22 40.16 15.00
207ARG 0 52.85 36.86 39.22 15.00
208GLY N 51.25 35.51 40.07 15.00
208GLY CA 50.55 35.33 38.81 15.00
208GLY C 49.86 33.97 38.78 15.00
208GLY O 49.80 33.27 39.79 15.00
209TYR N 49.32 33.60 37.63 15.00
209TYR CA 48.65 32.32 37.46 15.00
209TYR CB 47.14 32.43 37.78 15.00
64
CA 02301201 2000-02-04
WO 00/09653 PCTIUS98/14634
209TYR CG 46.30 33.17 36.74 15.00
209TYR CD1 45.95 32.54 35.54 15.00
209TYR CE1 45.27 33.23 34.53 15.00
209TYR CD2 45.95 34.51 36.91 15.00
209TYR CE2 45.27 35.20 35.90 15.00
209TYR CZ 44.94 34.55 34.72 15.00
209TYR OH 44.29 35.23 33.70 15.00
209TYR C 48.85 31.91 36.01 15.00
209TYR O 49.27 32.72 35.18 15.00
210ARG N 48.59 30.64 35.71 15.00
210ARG CA 48.70 30.12 34.35 15.00
210ARG CB 49.96 29.27 34.19 15.00
210ARG CG 51.15 30.04 33.66 15.00
210ARG CD 50.76 30.86 32.42 15.00
210ARG NE 51.90 31.36 31.65 15.00
210ARG CZ 53.02 31.88 32.16 15.00
210ARG NH1 53.21 31.98 33.47 15.00
210ARG NH2 53.96 32.33 31.32 15.00
210ARG C 47.48 29.26 34.05 15.00
210ARG O 47.02 28.47 34.88 15.00
211GLU N 46.94 29.41 32.84 15.00
211GLU CA 45.77 28.65 32.46 15.00
211GLU CB 44.83 29.54 31.65 15.00
211GLU CG 44.64 30.92 32.25 15.00
211GLU CD 43.20 31.38 32.17 15.00
211GLU OE1 42.37 30.81 32.91 15.00
211GLU OE2 42.90 32.31 31.39 15.00
211GLU C 46.17 27.46 31.63 15.00
211GLU O 46.84 27.61 30.61 15.00
212ILE N 45.81 26.27 32.09 15.00
212ILE CA 46.11 25.06 31.33 15.00
212ILE CB 45.66 23.79 '32.07 15.00
2I2ILE CG2 45.75 22.59 31.16 15.00
212ILE CG1 46.53 23.52 33.30 15.00
212ILE CD1 46.30 24.44 34.45 15.00
212ILE C 45.28 25.23 30.07 15.00
212ILE O 44.17 25.76 30.13 15.00
65
CA 02301201 2000-02-04
WO 00/09653 PCTIUS98/14634
213PRO N 45.82 24.86 28.90 15.00
213PRO CD 47.16 24.29 28.66 15.00
213PRO CA 45.09 25.00 27.64 15.00
213PRO CB 45.89 24.12 26.68 15.00
213PRO CG 47.28 24.36 27.14 15.00
213PRO C 43.64 24.56 ,27.74 15.00
213PRO 0 43.30 23.67 28.53 15.00
214GLU N 42.80 25.16 26.91 15.00
214GLU CA 41.35 24.90 26.88 15.00
214GLU CB 40.68 26.02 26.06 15.00
214GLU CG 39.26 26.39 26.48 15.00
214GLU CD 38.19 25.45 25.93 15.00
214GLU OE1 37.01 25.61 26.34 15.00
214GLU OE2 38.51 24.58 25.08 15.00
214GLU C 40.94 23.52 26.33 15.00
214GLU 0 41.21 23.20 25.18 15.00
215GLY N 40.28 22.73 27.17 15.00
215GLY CA 39.79 21.42 26.76 15.00
215GLY C 40.76 20.25 26.77 15.00
215GLY 0 40.41 19.13 27.15 15.00
216ASN N 41.98 20.51 .26.32 15.00
216ASN CA 43.03 19.50 26.24 15.00
216ASN CB 44.27 20.10 25.58 15.00
216ASN CG 45.47 19.19 25.64 15.00
216ASN OD1 45.36 18.02 26.01 15.00
216ASN ND2 46.63 19.73 25.30 15.00
216ASN C 43.40 18.87 27.59 15.00
216ASN O 43.84 19.56 28.50 15.00
217GLU N 43.29 17.55 27.67 15.00
217GLU CA 43.59 16.83 28.91 15.00
217GLU CB 42.71 15.60 29.05 15.00
217GLU CG 41.25 15.91 29.21 15.00
217GLU CD 40.42 14.67 29.07 15.00
217GLU OE1 39.86 14.22 30.09 15.00
217GLU OE2 40.34 14.14 27.93 15.00
217GLU C 45.04 16.41 .29.12 15.00
217GLU O 45.47 16.27 30.26 15.00
66
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14(34
218LYS N 45.77 16.14 28.04 15.00
218LYS CA 47.17 15.75 28.17 15.00
218LYS CB 47.80 15.49 26.80 15.00
218LYS CG 47.16 14.35 26.04 15.00
218LYS CD 47.73 14.24 24.62 15.00
218LYS CE 49.20 13.75 24.61 15.00
218LYS NZ 49.81 13.60 23.23 15.00
218LYS C 47.89 16.92 28.85 15.00
218LYS O 48.79 16.73 29.67 15.00
219ALA N 47.45 18.13 28.49 15.00
219ALA CA 48.01 19.34 29.04 15.00
219ALA CB 47.35 20.53 28.40 15.00
219ALA C 47.73 19.33 30.53 15.00
219ALA O 48.61 19.63 31.34 15.00
220LEU N 46.51 18.94 30.89 15.00
220LEU CA 46.14 18.91 32.29 15.00
220LEU CB 44.65 18.56 32.44 15.00
220LEU CG 44.05 18.37 33.84 15.00
220LEU CD1 44.26 19.58 34.76 15.00
220LEU CD2 42.58 18.06 33.71 15.00
220LEU C 47.02 17.91 33.02 15.00
220LEU O 47.55 18.22 34.08 15.00
221LYS N 47.24 16.76 32.40 15.00
221LYS CA 48.06 15.67 32.97 15.00
221LYS CB 48.19 14.53 31.95 15.00
221LYS CG 49.20 13.47 .32.33 15.00
221LYS CD 49.35 12.44 31.22 15.00
221LYS CE 50.40 11.43 31.59 15.00
221LYS NZ 50.20 10.97 33.00 15.00
221LYS C 49.45 16.11 33.37 15.00
221LYS O 49.92 15.76 34.45 15.00
222ARG N 50.12 16.82 32.48 15.00
222ARG CA 51.47 17.32 32.72 15.00
222ARG CB 52.06 17.83 31.42 15.00
222ARG CG 52.01 16.80 30.32 15.00
222ARG CD 53.17 15.85 30.42 15.00
222ARG NE 54.37 16.47 29.86 15.00
67
CA 02301201 2000-02-04
WO 00/09b53 PCT/US98/14C34
222ARG CZ 54.74 16.35 28.59 15.00
222ARG NH1 54.00 15.63 .27.74 15.00
222ARG NH2 55.86 16.92 28.16 15.00
222ARG C 51.41 18.43 33.76 15.00
222ARG O 52.15 18.39 34.75 15.00
223ALA N 50.48 19.36 33.58 15.00
223ALA CA 50.29 20.50 34.48 15.00
223ALA CB 48.96 21.16 34.19 15.00
223ALA C 50.33 20.07 35.94 15.00
223ALA 0 51.06 20.63 36.74 15.00
224VAL N 49.56 19.04 36.24 15.00
224VAL CA 49.47 18.50 37.58 15.00
224VAL CB 48.45 17.31 37.65 15.00
224VAL CG1 48.43 16.70 39.04 15.00
224VAL CG2 47.05 17.77 37.26 15.00
224VAL C 50.84 18.03 38.04 15.00
224VAL 0 51.42 18.62 .38.94 15.00
225ALA N 51.37 17.00 37.38 15.00
225ALA CA 52.66 16.43 37.76 15.00
225ALA CB 52.92 15.17 36.97 15.00
225ALA C 53.87 17.36 37.66 15.00
225ALA O 54.88 17.13 38.32 15.00
226ARG N 53.78 18.41 36.85 15.00
226ARG CA 54.90 19.33 36.70 15.00
226ARG CB 54.95 19.86 35.26 15.00
226ARG CG 56.31 20.45 34.85 15.00
226ARG CD 56.38 20.87 33.36 15.00
226ARG NE 56.18 19.76 32.40 15.00
226ARG CZ 56.94 18.66 32.34 15.00
226ARG NH1 57.95 18.49 33.18 15.00
226ARG NH2 56.67 17.72 31.43 15.00
.
226ARG C 54.84 20.49 37.69 15.00
226ARG O 55.89 20.95 38.15 15.00
227VAL N 53.63 20.93 38.03 15.00
227VAL CA 53.45 22.06 38.94 15.00
227VAL CB 52.54 23.12 38.31 15.00
227VAL CG1 52.50 24.36 39.19 15.00
68
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
227VAL CG2 53.01 23.45 36.92 15.00
227VAL C 52.87 21.76 40.33 15.00
227VAL O 53.17 22.46 41.29 15.00
228GLY N 51.99 20.78 40.41 15.00
228GLY CA 51.37 20.44 41.68 15.00
228GLY C 49.87 20.36 .41.46 15.00
228GLY O 49.45 20.39 40.30 15.00
229PRO N 49.03 20.27 42.51 15.00
229PRO CD 49.35 20.33 43.95 15.00
229PRO CA 47.58 20.19 42.31 15.00
229PRO CB 47.04 20.14 43.74 15.00
229PRO CG 48.06 20.86 44.52 15.00
229PRO C 47.05 21.39 41,55 15.00
229PRO O 47.31 22.54 41.92 15.00
230VAL N 46.34 21.13 40.46 15.00
230VAL CA 45.75 22.3.9 39.65 15.00
230VAL CB 45.95 21.89 38.16 15.00
230VAL CG1 45.22 22.89 37.30 15.00
230VAL CG2 47.40 21.90 37.84 15.00
230VAL C 44.25 22.37 39.94 15.00
230VAL O 43.59 21.42 .40.37 15.00
231SER N 43.74 23.58 39.76 15.00
231SER CA 42.33 23.89 39.96 15.00
231sER CB 42.17 25.38 40.29 15.00
231SER OG 42.60 25.66 41.61 15.00
231SER C 41.54 23.56 38.69 15.00
232SER O 42.02 23.82 37.58 15.00
232VAL N 40.35 22.96 38.84 15.00
232VAL CA 39.50 22.62 37.69 15.00
232VAL CB 39.65 21.13 37.25 15.00
232VAL CG1 41.00 20.91 36.59 15.00
232VAL CG2 39.47 20.20 38.43 15.00
232VAL C 38.01 22.93 37.92 15.00
232VAL O 37.59 23.17 39.06 15.00
233ALA N 37.24 22.97 36.84 15.00
233ALA CA 35.81 23.26 36.88 15.00
233ALA CB 35.51 24.52 36.09 15.00
69
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
233 ALA C 35.12 22.07 36.25 15.00
233 ALA O 35.63 21.51 35.29 15.00
234 ILE N 33.95 21.70 36.77 15.00
234 ILE CA 33.20 20.54 36.28 15.00
234 ILE CB 33.48 19.29 37.16 15.00
234 ILE CG2 34.83 18.69 36.84 15.00
234 ILE CG1 33.34 29.68 38.65 15.00
234 ILE CD1 33.22 18.53 39.59 15.00
234 ILE C 31.69 20.74 36.29 15.00
234 ILE O 31.19 21.79 36.70 15.00
235 ASP N 30.97 19.68 35.90 15.00
235 ASP CA 29.52 19.64 35.85 15.00
235 ASP CB 29.06 19.03 34.53 15.00
235 ASP CG 27.57 19.14 34.32 15.00
235 ASP ODl 26.80 19.01 35.29 15.00
235 ASP OD2 27.17 19.35 33.15 15.00
235 ASP C 29.05 18.74 36.99 15.00
235 ASP O 28.88 17.54 36.80 15.00
236 ALA N 28.83 19.32 38.16 15.00
236 ALA CA 28.40 18.55 39.32 15.00
236 ALA CB 29.13 19.06 40.56 15.00
236 ALA C 26.88 18.61 39.53 15.00
236 ALA 0 26.39 18.67 40.67 15.00
237 SER N 26.12 18.60 38.43 15.00
237 SER CA 24.66 18.64 38.52 15.00
.
237 SER CB 24.05 19.01 37.17 15.00
237 SER OG 24.41 20.33 36.81 15.00
237 SER C 24.16 17.27 38.93 15.00
237 SER 0 23.69 17.09 40.05 15.00
238 LEU N 24.36 16.31 38.05 15.00
238 LEU CA 23.94 14.92 38.23 15.00
238 LEU CB 24.85 14.01 37.41 15.00
238 LEU CG 24.71 14.03 35.89 15.00
238 LEU CD1 24.06 15.31 35.38 15.00
238 LEU CD2 26.08 13.85 35.28 15.00
238 LEU C 23.82 14.36 39.65 15.00
238 LEU O 24.67 24.62 40.51 15.00
70
CA 02301201 2000-02-04
WO 00/09653 PCT/US98114634
239 THR 22.78 13.58 39.89 15.00
N
239 THR CA 22.57 12.97 41.20 15.00
239 THR CB 21.14 12.40 41.34 15.00
239 THR OG1 20.58 12.17 40.04 15.00
239 THR CG2 20.25 13.37 42.13 15.00
239 THR C 23.59 11.85 41.41 15.00
239 THR O 24.04 11.62 42.54 15.00
240 SER N 23.97 11.18 40.33 15.00
240 SER CA 24.95 10.12' 40.39 15.00
240 SER CB 25.24 9.60 38.99 15.00
240 SER OG 25.34 10.66 38.08 15.00
240 SER C 26.22 10.69 41.02 15.00
240 SER O 26.97 9.99 41.69 15.00
241 PHE N 26.45 11.9$ 40.79 15.00
241 PHE CA 27.58 12.67 .41.37 15.00
241 PHE CB 27.83 13.99 40.63 15.00
241 PHE CG 29.04 14.74 41.12 15.00
241 PHE CD1 30.17 14.85 40.32 15.00
241 PHE CD2 29.03 15.38 42.35 15.00
241 PHE CE1 31.27 15.59 40.74 15.00
241 PHE CE2 30.12 16.12 42.77 15.00
241 PHE CZ 31.24 16.22 41.96 15.00
241 PHE C 27.26 12.95 42.84 15.00
241 PHE 0 27.92 12.42 43.73 15.00
242 GLN N 26.20 13.71 43.09 15.00
242 GLN CA 25.78 14.08 44.44 15.00
242 GLN CB 24.36 14.62 44.42 15.00
242 GLN CG 23.88 15.20 45.73 15.00
242 GLN CD 22.37 15.17 45.8$ 15.00
242 GLN OE1 21.83 15.59 46.$9 25.00
242 GLN NE2 21.68 14.65 44.87 15.00
242 GLN C 25.86 12.98 45.49 15.00
242 GLN 0 26.05 13.27 46.69 15.00
243 PHE N 25.70 11.73 45.06 15.00
243 PHE CA 25.72 10.58 45.97 15.00
243 PHE 24.45 9.74 45.79 15.00
CB
243 23.22 10.44 46.23 15.00
PHE
CG
71
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14b34
243 PHE 22.95 10.59 47.58 15.00
CD1
243 PHE 22.36 11.00 45.30 15.00
CD2
243 PHE 21.85 11.31 48.01 15.00
CE1
243 PHE CE2 21.26 11.72 45.72 15.00
243 PHE CZ 21.00 11.88 .47.08 15.00
243 PHE C 26.93 9.66 45.86 15.00
243 PHE O 26.97 8.63 46.54 15.00
244 TYR N 27.88 10.00 44.99 15.00
244 TYR CA 29.09 9.20 44.81 15.00
244 TYR CB 30.17 10.02 44.09 15.00
244 TYR CG 31.52 9.34 44.06 15.00
244 TYR CD1 31.86 8.43 43.05 15.00
244 TYR CE1 33.07 7.72 43.09 15.00
244 TYR CD2 32.42 9.52 45.10 15.00
244 TYR CE2 33.62 8.82 45.16 15.00
244 TYR CZ 33.94 7.92 44.16 15.00
244 TYR OH 35.12 7.20 44.29 15.00
244 TYR C 29.63 8.64 46.13 15.00
244 TYR O 29.53 9.28 47.18 15.00
'
245 SER N 30.20 7.44 46.06 15.00
245 SER CA 30.78 6.78 47.22 15.00
245 SER CB 29.88 5.63 47.69 15.00
245 SER OG 30.30 5.06 48.92 15.00
245 SER C 32.19 6.25 46.92 15.00
245 SER O 33.16 6.62 47.60 15.00
246 LYS N 32.31 5.39 45.91 15.00
246 LYS CA 33.61 4.81 45.57 15.00
246 LYS CB 33.82 3.50 46.33 15.00
246 LYS CG 34.29 3.71 47.75 15.00
246 LYS CD 33.63 2.74 48.71 15.00
246 LYS CE 34.06 3.03 50.15 15.00
246 LYS NZ 33.94 4.48 50.54 15.00
246 LYS C 33.71 4.56 44.08 15.00
246 LYS 0 32.73 4.75 .43.35 15.00
247 GLY N 34.90 4.18 43.62 15.00
247 GLY CA 35.09 3.90 42.21 15.00
247 C 35.36 5.08 41.29 15.00
GLY
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247 GLY O 35.42 6.22 41.72 15.00
248 VAL N 35.53 4.77 40.02 15.00
248 VAL CA 35.81 5.78 39.01 15.00
248 VAL CB 36.66 5.18 37.87 15.00
248 VAL CG1 36.96 6.23 36.82 15.00
248 VAL CG2 37.96 4.62 38.43 15.00
248 VAL C 34.47 6.26 38.47 15.00
248 VAL O 33.75 5.51 37.82 15.00
249 TYR N 34.15 7.51 38.77 15,00
249 TYR CA 32.90 8.12 38.37 15.00
249 TYR CB 32.58 9.29 39.30 15.00
249 TYR CG 31.31 10.02 38.94 15.00
249 TYR CD1 30.12 9.78 39.63 15.00
249 TYR CE1 28.94 10.43 39.27 15.00
249 TYR CD2 31.28 10.95 37.90 15.00
249 TYR CE2 30.11 11.59 37.54 15.00
249 TYR CZ 28.95 11.34 38.22 15.00
249 TYR OH 27.80 12.00 37.86 15.00
249 TYR C 32.80 8.59 36.92 15.00
249 TYR O 33.42 9.56 36.52 15.00
250 TYR N 31.91 7.95 36.18 15.00
250 TYR CA 31.66 8.32 34.80 15.00
250 TYR CB 32.39 7.39 33.84 15.00
250 TYR CG 32.13 7.70 32.38 15.00
250 TYR CD1 32.16 9.01 31.91 15.00
250 TYR CE1 31.94 9.28 30.57 15.00
250 TYR CD2 31.89 6.67 31.48 15.00
250 TYR CE2 31.68 6.93 30.14 15.00
250 TYR CZ 31.70 8.23 29.69 15.00
250 TYR OH 31.47 8.48 28.36 15.00
250 TYR C 30.17 8.21 34.56 15.00
250 TYR O 29.58 7.14 34.75 15.00
251 ASP N 29.54 9.33 34.23 15.00
251 ASP CA 28.12 9.34 33.96 15.00
251 ASP CB 27.37 10.20 34.98 15.00
251 ASP CG 25.84 10.11 34.84 15.00
251 ASP OD1 25.34 9.43 33.91 15.00
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251 ASP OD2 25.13 10.73 35.65 15.00
251 ASP C 27.98 9.93 32.57 15.00
251 ASP O 28.34 11.08 32.35 15.00
252 GLU N 27.39 9.15 31.67 15.00
252 GLU CA 27.18 9.55 30.28 15.00
252 GLU CB 26.55 8.41 29.45 15.00
252 GLU CG 26.94 6.97 29.83 15.00
252 GLU CD 26.21 6.45 31.07 15.00
252 GLU OE1 25.02 6.81 31.27 15.00
252 GLU OE2 26.82 5.71 31.87 15.00
252 GLU C 26.27 10.78 30.18 15.00
252 GLU O 25.85 11.15 29.08 15.00
253 SER N 25.95 11.39 31.32 15.00
253 SER CA 25.06 12.53 31.35 15.00
253 SER CB 23.92 12.21 32.30 15.00
253 SER OG 23.50 10.85 32.13 15.00
253 SER C 25.71 13.86 31.72 15.00
253 SER O 25.08 14.92 31.61 15.00
254 CYS N 26.95 13.81 32.21 15.00
254 CYS CA 27.69 15.02 32.58 15.00
254 CYS C 27.77 15.85 31.30 15.00
254 CYS O 27.93 15.30 30.21 15.00
254 CYS CB 29.12 14.65 33.04 15.00
254 CYS SG 29.66 15.23 34.70 15.00
255 ASN N 27.60 17.16 31.41 15.00
255 ASN CA 27.69 18.00 30.23 15.00
255 ASN CB 26.41 18.79 30.01 15.00
255 ASN CG 26.54 19.76 28.85 15.00
255 ASN OD1 26.34 20.96 29.02 15.00
255 ASN ND2 26.94 19.25 27.69 15.00
255 ASN C 28.86 18.98 30.26 15.00
255 ASN 0 28.94 19.82 31.14 15.00
256 SER N 29.71 18.89 29.24 15.00
256 SER CA 30.88 19.75 29.10 15.00
256 SER CB 31.66 19.38 27.83 15.00
256 SER OG 32.00 18.00 27.83 15.00
256 SER C 30.57 21.25 29.07 15.00
74
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256 SER O 31.42 22.06 29.45 15.00
257 ASP N 29.39 21.63 28.59 15.00
257 ASP CA 29.00 23.04 28.53 15.00
257 ASP CB 27.96 23.26 .27.44 15.00
257 ASP CG 28.50 23.00 26.05 15.00
257 ASP OD1 28.50 21.82 25.63 15.00
257 ASP OD2 28.91 23.96 25.36 15.00
257 ASP C 28.47 23.61 29.85 15.00
257 ASP 0 28.51 24.82 30.07 15.00
258 ASN N 27.95 22.76 30.74 15.00
258 ASN CA 27.41 23.23 32.01 15.00
258 ASN CB 26.15 22.43 32.39 15.00
258 ASN CG 25.57 22.82 33.75 15.00
258 ASN OD1 25.50 24.00 34.10 15.00
258 ASN ND2 25.15 21.83 34.53 15.00
258 ASN C 28.45 23.14 33.14 15.00
258 ASN 0 28.27 22.37 34.09 15.00
259 LEU N 29.53 23.90 33.03 15.00
259 LEU CA 30.56 23.89 34.06 15.00
259 LEU CB 31.79 24.65 33.58 15.00
259 LEU CG 32.55 24.17 32.34 15.00
259 LEU CD1 33.69 25.13 32.06 15.00
259 LEU CD2 33.08 22.77 32.57 15.00
259 LEU C 29.99 24.59 35.28 15.00
259 LEU O 29.76 25.80 35.25 15.00
260 ASN N 29.75 23.87 36.36 15.00
260 ASN CA 29.17 24.51 37.52 15.00
260 ASN CB 27.70 24.15 37.61 15.00
260 ASN CG 27.47 22.66 37.68 15.00
260 ASN OD1 28.02 21.99 38.54 15.00
260 ASN ND2 26.68 22.14 36.75 15.00
260 ASN C 29.82 24.27 38.87 15.00
260 ASN O 29.30 24.73 39.88 15.00
261 HIS N 30.91 23.52 '38.92 15.00
261 HIS CA 31.57 23.30 40.20 15.00
261 HIS CB 31.25 21.91 40.75 15.00
261 HIS CG 31.51 21.75 42.23 15.00
75
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261 CD2 31.94 20.69 42.95 15.00
HIS
261 ND1 31.30 22.77 43.13 15.00
HIS
261 CE1 31.59 22.34 44.35 15.00
HIS
261 HIS NE2 31.98 21.08 44.26 15.00
261 HIS C 33.07 23.51 40.04 15.00
261 HIS O 33.61 23.35 38.95 15.00
262 ALA N 33.72 23.92 41.14 15.00
262 ALA CA 35.15 24.16 41.18 15.00
262 ALA CB 35.41 25.58 .41.62 15.00
262 ALA C 35.81 23.18 42.16 15.00
262 ALA O 35.49 23.18 43.35 15.00
263 VAL N 36.75 22.39 42.66 15.00
263 VAL CA 37.44 21.39 42.46 15.00
263 VAL CB 36.85 19.99 42.18 15.00
263 VAL CG1 35.47 19.89 42.73 15.00
263 VAL CG2 36.82 19.71 40.69 15.00
263 VAL C 38.97 21.34 42.25 15.00
263 VAL O 39.51 21.84 41.26 15.00
264 LEU N 39.66 20.70 43.18 25.00
264 LEU CA 41.11 20.58 43.16 15.00
264 LEU CB 41.65 20.69 44.59 15.00
264 LEU CG 43.12 21.07 44.77 15.00
264 LEU CD1 43.26 22.53 44.44 15.00
264 LEU CD2 43.59 20.81 46.19 15.00
264 LEU C 41.59 19.25 42.56 15.00
264 LEU O 41.33 18.20 43.14 15.00
265 ALA N 42.28 19.30 41.43 15.00
265 ALA CA 42.81 18.09 40.83 15.00
265 ALA CB 43.08 18.31 39.37 15.00
265 ALA C 44.12 17.84 41.56 15.00
265 ALA 0 45.02 18.66 41.48 15.00
266 VAL 44.22 16.74 42.30 15.00
N
266 VAL 45.45 16.43 43.03 15.00
CA
266 VAL 45.21 16.32 44.55 15.00
CB
266 VAL 44.51 17.56 45.07 15.00
CG1
266 VAL 44.42 15.07 44.88 15.00
CG2
266 46.21 15.19 42.57 15.00
VAL
C
76
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266VAL O 47.11 14.72 43.27 15.00
267GLY N 45.85 14.65 41.42 15.00
267GLY CA 46.54 13.48 40.92 15.00
267GLY C 45.82 12.84 39.77 15.00
267GLY O 44.81 13.37 39.29 15.00
268TYR N 46.32 11.71 39.29 15.00
268TYR CA 45.70 10.99 38.19 15.00
268TYR CB 46.02 11.65 36.85 15.00
268TYR CG 47.49 11.81 36.57 15.00
268TYR CD1 48.25 10.74 36.09 15.00
268TYR CE1 49.58 10.89 35.79 15.00
268TYR CD2 48.13 13.03 36.74 15.00
268TYR CE2 49.48 13.19 .36.44 15.00
268TYR CZ 50.19 12.11 35.96 15.00
268TYR OH 51.50 12.25 35.62 15.00
268TYR C 46.17 9.55 38.24 15.00
268TYR O 46.87 9.17 39.19 15.00
269GLY N 45.75 8.72 37.29 15.00
269GLY CA 46.19 7.34 37.30 15.00
269GLY C 45.08 6.30 37.24 15.00
269GLY 0 43.92 6.58 37.55 15.00
270ILE N 45.47 5.08 36.88 15.00
270ILE CA 44.56 3.96 36.74 15.00
270ILE CB 45.22 2.86 35.89 15.00
270ILE CG2 44.36 1.61 35.83 15.00
270ILE CG1 45.43 3.39 34.48 15.00
270ILE CD1 45.60 2.31 33.44 15.00
270ILE C 44.09 3.43 .38.10 15.00
270ILE 0 44.69 3.75 39.13 15.00
271GLN N 42.96 2.71 38.10 15.00
271GLN CA 42.38 2.14 39.33 15.00
271GLN CB 41.10 2.86 39.75 15.00
271GLN CG 40.45 2.30 41.01 15.00
271GLN CD 41.33 2.44 42.25 15.00
271GLN OE1 40.96 3.12 43.21 15.00
271GLN NE2 42.49 1.80 42.23 15.00
271GLN C 42.07 0.65 39.23 15.00
77
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271 GLN 0 42.72 -0.16 39.88 15.00
272 LYS N 41.03 0.31 38.47 15.00
272 LYS CA 40.67 -1.10 .38.30 15.00
272 LYS CB 39.32 -1.37 38.96 15.00
272 LYS CG 39.13 -2.79 39.44 15.00
272 LYS CD 39.79 -3.05 40.79 15.00
272 LYS CE 39.57 -4.51 41.20 15.00
272 LYS NZ 40.01 -4.82 42.59 15.00
272 LYS C 40.60 -1.36 36.80 15.00
272 LYS O 39.81 -2.17 36.33 15.00
273 GLY N 41.48 -0.70 36.06 15.00
273 GLY CA 41.50 -0.86 34.62 15.00
273 GLY C 41.01 0.42 33.97 15.00
273 GLY 0 41.00 0.56 32.74 15.00
274 ASN N 40.62 1.37 34.81 15.00
274 ASN CA 40.11 2.65 34.34 15.00
274 ASN CB 38.74 2.96 34.97 15.00
274 ASN CG 37.76 1.82 34.81 15.00
274 ASN OD1 37.24 1.58 33.73 15.00
274 ASN ND2 37.49 1.11 35.90 15.00
274 ASN C 41.08 3.73 34.80 15.00
274 ASN 0 41.52 3.71 35.96 15.00
275 LYS N 41.43 4.63 33.89 15.00
275 LYS CA 42.32 5.74 34.22 15.00
275 LYS CB 42.98 6.29 32.96 15.00
275 LYS CG 43.74 5.26 32.16 15.00
275 LYS CD 44.26 5.91 30.91 15.00
275 LYS CE 44.42 4.88 29.82 15.00
275 LYS NZ 44.59 5.58 28.51 15.00
275 LYS C 41.43 6.80 34.83 15.00
275 LYS 0 40.43 7.19 34.23 15.00
276 HIS N 41.82 7.31 35.99 15.00
276 HIS CA 41.01 8.30 36.70 15.00
276 HIS CB 40.50 7.68 38.01 15.00
276 HIS CG 41.58 7.33 39.00 15.00
276 HIS CD2 42.30 8.10 39.86 15.00
276 HIS ND1 41.95 6.03 39.27 15.00
78
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276 HIS CE1 42.82 6.00 40.26 15.00
276 HIS NE2 43.05 7.25 40.63 15.00
276 HIS C 41.73 9.59 37.01 15.00
276 HIS 0 42.92 9.73 36.73 15.00
277 TRP N 40.98 10.53 37.57 15.00
277 TRP CA 41.52 11.81 37.99 15.00
277 TRP CB 40.75 12.96 37.34 15.00
277 TRP CG 41.22 13.34 35.99 15.00
277 TRP CD2 42.48 13.95 35.66 15.00
277 TRP CE2 42.48 14.17 34.27 15.00
277 TRP CE3 43.60 14.33 36.41 15.00
277 TRP CD1 40.54 13.22 34.81 15.00
277 TRP NE1 41.29 13.71 33.77 15.00
277 TRP CZ2 43.57 14.74 33.61 15.00
277 TRP CZ3 44.67 14.90 35.75 15.00
277 TRP CH2 44.65 15.11 34.36 15.00
277 TRP C 41.26 11.82 39.49 15.00
277 TRP O 40.31 11.17 39.94 15.00
278 ILE N 42.12 12.46 40.29 15.00
278 ILE CA 41.86 12.52 41.73 15.00
278 ILE CB 43.07 12.15 42.58 15.00
278 ILE CG2 42.63 11.96 44.03 15.00
278 ILE CG1 43.70 10.87 42.05 15.00
278 ILE CD1 44.89 10.39 42.85 15.00
278 ILE C 41.44 13.94 42.05 15.00
278 ILE O 42.23 14.87 41.94 15.00
279 ILE N 40.17 14.10 42.41 15.00
279 ILE CA 39.63 15.42 42.69 15.00
279 ILE CB 38.48 15.74 41.71 15.00
279 ILE CG2 37.57 16.79 42.28 15.00
279 ILE CG1 39.07 16.16 40.35 15.00
279 ILE CD1 38.05 16.69 39.36 15.00
279 ILE C 39.17 15.62 44.13 15.00
279 ILE O 38.55 14.74 44.73 15.00
280 LYS N 39.51 16.79 44.66 25.00
280 LYS CA 39.17 17.17 46.01 15.00
280 LYS CB 40.34 17.93 46.63 15.00
79
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280 LYS CG 40.12 18.37 48.07 15.00
280 LYS CD 41.34 19.11 48.58 15.00
280 LYS CE 41.13 19.63 49.98 15.00
280 LYS NZ 42.34 20.34 50.48 15.00
280 LYS C 37.97 18.09 45.95 15.00
280 LYS O 37.95 19.07 45.20 15.00
281 ASN N 36.94 17.76 46.74 15.00
281 ASN CA 35.74 18.57 46.76 15.00
281 ASN CB 34.48 17.70 46.73 15.00
281 ASN CG 33.38 18.32 45.91 15.00
281 ASN OD1 32.82 19.36 46.27 15.00
281 ASN ND2 33.09 17.72 44.77 15.00
281 ASN C 35.73 19.48 '47.98 15.00
281 ASN O 36.70 19.50 48.74 15.00
282 SER N 34.62 20.20 48.15 15.00
282 SER CA 34.44 21.13 49.26 15.00
282 SER CB 34.30 22.55 48.70 15.00
282 SER OG 34.88 22.65 47.41 15.00
282 SER C 33.19 20.80 50.05 15.00
282 SER O 32.67 21.65 50.76 15.00
283 TRP N 32.71 19.57 49.93 15.00
283 TRP CA 31.49 19.17 50.65 15.00
283 TRP CB 30.50 18.46 49.71 15.00
283 TRP CG 30.00 19.32 48.58 15.00
283 TRP CD2 29.39 18.88 47.37 15.00
283 TRP CE2 29.11 20.02 46.61 15.00
283 TRP CE3 29.06 17.62 46.87 15.00
283 TRP CD1 30.06 20.69 48.50 15.00
283 TRP NE1 29.52 21.11 47.32 15.00
283 TRP CZ2 28.51 19.94 45.35 15.00
283 TRP CZ3 28.47 17.54 45.62 15.00
283 TRP CH2 28.19 18.70 44.88 15.00
283 TRP C 31.75 18.34 51.92 15.00
283 TRP O 31.01 17.41 52.22 15.00
284 GLY N 32.76 18.73 52.68 15.00
284 GLY CA 33.08 18.03 53.91 15.00
284 GLY C 33.53 16.59 53.76 15.00
80
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284 GLY O 33.58 16.05 52.66 15.00
285 GLU N 33.88 15.99 54.89 15.00
285 GLU CA 34.34 14.61 54.97 15.00
285 GLU CB 34.76 14.28 56.41 15.00
285 GLU CG 33.79 14.81 57.52 15.00
285 GLU CD 33.04 13.71 58.29 15.00
285 GLU OE1 33.21 12.51 57.98 15.00
285 GLU OE2 32.28 14.05 59.23 15.00
285 GLU C 33.33 13.56 54.51 15.00
285 GLU O 33.65 12.70 53.68 15.00
286 ASN N 32.13 13.60 55.09 15.00
286 ASN CA 31.05 12.66 54.80 15.00
286 ASN CB 29.89 12.86 55.78 15.00
286 ASN CG 30.14 12.22 57.12 15.00
286 ASN OD1 29.89 12.83 58.16 15.00
286 ASN ND2 30.63 10.98 57.12 15.00
286 ASN C 30.47 12.72 53.38 15.00
286 ASN O 29.25 12.86 53.23 15.00
287 TRP N 31.31 12.62 52.36 15.00
287 TRP CA 30.83 12.63 50.98 15.00
287 TRP CB 30.76 14.06 50.45 15.00
287 TRP CG 30.33 14.12 49.00 15.00
287 TRP CD2 31.18 14.10 47.84 15.00
287 TRP CE2 30.35 14.15 46.71 15.00
287 TRP CE3 32.57 14.04 47.66 15.00
287 TRP CD1 29.05 14.18 48.53 15.00
287 TRP NE1 29.05 14.21 47.16 15.00
287 TRP CZ2 30.85 14.14 45.40 15.00
287 TRP CZ3 33.07 14.03 46.35 15.00
287 TRP CH2 32.21 14.08 45.24 15.00
287 TRP C 31.81 11.83 50.15 15.00
287 TRP O 33.01 11.85 '50.44 15.00
288 GLY N 31.33 11.14 49.12 15.00
288 GLY CA 32.22 10.36 48.28 15.00
288 GLY C 33.18 9.53 49.11 15.00
288 GLY O 32.85 9.17 50.24 15.00
289 ASN N 34.39 9.29 48.60 15.00
81
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289 ASN CA 35.36 8.48 49.34 15.00
289 ASN CB 36.28 7.74 48.38 15.00
289 ASN CG 37.06 6.65 49.07 15.00
289 ASN OD1 37.13 6.61 50.30 15.00
289 ASN ND2 37.63 5.75 48.28 15.00
289 ASN C 36.20 9.30 50.31 15.00
289 ASN O 37.35 9.62 50.03 15.00
290 LYS N 35.60 9.63 51.45 15.00
290 LYS CA 36.27 10.41 52.48 15.00
290 LYS CB 37.47 9.64 53.05 15.00
290 LYS CG 37.97 10.14 54.42 15.00
290 LYS CD 37.30 9.40 55.60 15.00
290 LYS CE 37.89 9.80 56.97 15.00
290 LYS NZ 37.52 11.19 57.40 15.00
290 LYS C 36.67 11.81 51.97 15.00
290 LYS O 37.78 12.29 52.23 15.00
291 GLY N 35.78 12.46 51.23 15.00
291 GLY CA 36.06 13.80 50.73 15.00
291 GLY C 36.44 13.95 49.26 15.00
291 GLY O 36.10 14.95 48.63 15.00
292 TYR N 37.14 12.97 48.70 15.00
292 TYR CA 37.56 13.06 47.31 15.00
292 TYR CB 39.02 12.60 47.16 15.00
292 TYR CG 40.02 13.32 48.04 15.00
292 TYR CD1 40.15 13.01 49.39 15.00
292 TYR CE1 41.03 13.70 50.20 15.00
292 TYR CD2 40.81 14.35 47.53 15.00
292 TYR CE2 41.69 15.04 48.34 15.00
292 TYR CZ 41.79 14.71 49.67 15.00
292 TYR OH 42.66 15.41 50.48 15.00
292 TYR C 36.67 12.18 46.45 15.00
292 TYR O 35.79 11.46 46.95 15.00
293 ILE N 36.93 12.21 45.15 15.00
293 ILE CA 36.19 11.43 44.19 15.00
293 ILE CB 34.98 12.20 43.68 15.00
293 ILE CG2 35.43 13.55 43.14 15.00
293 ILE CG1 34.25 11.39 42.62 15.00
82
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293 ILE CD2 33.05 12.09 42.03 15.00
293 ILE C 37.13 11.19 43.02 15.00
293 ILE O 37.95 12.05 42.70 15.00
294 LEU N 37.05 10.00 42.42 15.00
294 LEU CA 37.88 9.64 41.28 15.00
294 LEU CB 38.29 8.18 41.37 15.00
294 LEU CG 39.48 7.75 42.21 15.00
294 LEU CD1 39.28 8.16 43.66 15.00
294 LEU CD2 39.61 6.25 42.11 15.00
294 LEU C 37.04 9.80 40.04 15.00
294 LEU O 36.16 9.00 39.80 15.00
295 MET N 37.31 10.81 39.24 15.00
295 MET CA 36.51 11.00 38.03 15.00
295 MET CB 36.25 12.49 37.81 15.00
295 MET CG 35.59 13.13 39.01 15.00
295 MET SD 35.02 14.79 38.73 15.00
295 MET CE 33.94 14.53 37.34 15.00
295 MET C 37.18 10.37 36.83 15.00
29S MET O 38.41 10.29 36.77 15.00
296 ALA N 36.38 9.92 35.86 15.00
296 ALA CA 36.92 9.29 34.65 15.00
296 ALA CB 35.81 8.97 33.68 15.00
296 ALA C 37.94 10.22 34.01 15.00
296 ALA O 37.72 11.44 33.93 15.00
297 ARG N 39.04 9.62 33.55 15.00
.
297 ARG CA 40.14 10.38 32.96 15.00
297 ARG CB 41.48 9.83 33.45 15.00
297 ARG CG 42.65 10.70 33.07 15.00
297 ARG CD 43.59 10.86 34.23 15.00
297 ARG NE 44.34 9.64 34.52 15.00
297 ARG CZ 45.14 9.04 33.64 15.00
297 ARG NH1 45.30 9.53 32.41 15.00
297 ARG NH2 45.85 7.98 34.03 15.00
297 ARG C 40.20 10.55 31.45 15.00
297 ARG 0 39.79 11.60 30.94 15.00
298 ASN N 40.79 9.59 30.75 25.00
298 ASN CA 40.89 9.73 29.30 15.00
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298 42.14 9.04 28.77 15.00
ASN
CB
298 CG 43.39 9.92 28.86 15.00
ASN
298 ASN OD1 43.35 11.11 28.55 15.00
298 ASN ND2 44.50 9.33 29.30 15.00
298 ASN C 39.64 9.27 28.58 15.00
298 ASN O 39.66 8.32 27.79 15.00
299 LYS N 38.53 9.94 28.89 15.00
299 LYS CA 37.22 9.70 28.31 15.00
299 LYS CB 36.27 9.08 29.34 15.00
299 LYS CG 36.69 7.69 29.84 15.00
299 LYS CD 36.83 6.71 28.68 15.00
299 LYS CE 35.49 6.19 28.19 15.00
299 LYS NZ 34.95 5.23 29.18 15.00
299 LYS C 36.72 11.07 27.91 15.00
299 LYS 0 36.49 11.92 .28.76 15.00
300 ASN N 36.63 11.30 26.61 15.00
300 ASN CA 36.21 12.55 26.00 15.00
300 ASN CB 35.05 12.31 25.04 15.00
300 ASN CG 35.52 11.73 23.73 15.00
300 ASN OD1 36.22 10.72 23.71 15.00
300 ASN ND2 35.15 12.38 22.63 15.00
300 ASN C 35.95 13.80 26.83 15.00
300 ASN O 34.96 14.50 26.62 15.00
301 ASN N 36.88 14.12 27.71 15.00
301 ASN CA 36.77 15.29 28.56 15.00
301 ASN CB 36.73 16.54 27.67 15.00
301 ASN CG 36.90 17.82 28.45 15.00
301 ASN OD1 37.58 17.85 29.49 15.00
301 ASN ND2 36.30 18.90 27.96 15.00
.
301 ASN C 35.51 15.17 29.41 15.00
301 ASN O 34.67 16.08 29.47 15.00
302 ALA N 35.37 14.03 30.08 15.00
302 ALA CA 34.21 13.77 30.93 15.00
302 ALA CB 34.41 12.48 31.70 15.00
302 ALA C 33.97 14.93 31.89 15.00
302 ALA O 34.91 15.48 32.46 15.00
303 CYS 32.72 15.35 32.01 15.00
N
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303 CYS CA 32.35 16.45 32.89 15.00
303 CYS C 33.20 17.67 32.59 25.00
303 CYS O 33.24 18.63 33.37 15.00
303 CYS CB 32.55 16.04 34.35 15.00
303 CYS SG 31.58 14.59 34.86 15.00
304 GLY N 33.86 17.64 31.43 15.00
304 GLY CA 34.72 18.72 31.02 15.00
304 GLY C 35.77 19.04 32.05 15.00
304 GLY O 36.01 20.22 32.32 15.00
305 ILE N 36.43 18.03 32.61 15.00
305 ILE CA 37.44 18.30 33.62 15.00
305 zLE CB 37.95 17.03 34.32 15.00
305 ILE CG2 38.74 16.18 33.37 15.00
305 ILE CG1 38.78 17.42 35.54 15.00
305 ILE CD1 39.35 16.26 36.28 15.00
305 ILE C 38.60 19.14 33.08 15.00
305 ILE O 39.19 19.95 33.80 15.00
306 ALA N 38.85 19.03 31.79 15.00
306 ALA CA 39.93 19.80 31.19 15.00
306 ALA CB 40.64 18.95 30.18 15.00
306 ALA C 39.42 21.08 30.54 15.00
306 ALA O 40.01 21.56 29.58 15.00
307 ASN N 38.35 21.67 31.07 15.00
307 ASN CA 37.81 22.89 30.49 15.00
307 ASN CB 36.29 22.91 30.59 15.00
307 ASN CG 35.60 22.56 29.28 15.00
307 ASN OD1 36.23 22.08 28.34 15.00
307 ASN ND2 34.30 22.79 29.23 15.00
307 ASN C 38.35 24.20 31.03 15.00
307 ASN 0 38.76 25.07 30.27 15.00
308 LEU N 38.30 24.36 32.35 15.00
308 LEU CA 38.76 25.60 32.99 15.00
308 LEU CB 37.56 26.32 33.61 15.00
308 LEU CG 36.91 27.48 .32.85 15.00
308 LEU CD1 36.75 27.18 31.36 15.00
308 LEU CD2 35.55 27.76 33.51 25.00
308 LEU C 39.79 25.36 34.06 15.00
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308 LEU 39.57 25.69 35.23 15.00
O
309 ALA N 40.94 24.85 33.64 15.00
309 ALA CA 42.03 24.54 34.57 15.00
309 ALA CB 42.75 23.29 34.11 15.00
309 ALA C 43.01 25.69 34.70 15.00
309 ALA O 43.30 26.36 33.71 15.00
310 SER N 43.56 25.88 35.90 15.00
310 SER CA 44.53 26.93 36.16 15.00
310 SER CB 43.85 28.30 36.08 15.00
310 SER OG 42.70 28.40 36.90 15.00
310 SER C 45.23 26.74 37.50 15.00
310 SER O 44.78 25.96 38.34 15.00
311 PHE N 46.36 27.40 37.67 15.00
311 PHE CA 47.12 27.31 38.92 15.00
311 PHE CB 47.96 26.03 38.93 15.00
311 PHE CG 49.01 25.98 37.85 15.00
311 PHE CD1 50.25 26.56 38.04 15.00
311 PHE CD2 48.74 25.37 36.64 15.00
311 PHE CE1 51.21 26.54 37.04 15.00
311 PHE CE2 49.70 25.34 35.64 15.00
311 PHE CZ 50.93 25.93 35.85 15.00
311 PHE C 47.99 28.54 39.03 15.00
311 PHE O 48.41 29.12 38.02 15.00
312 PRO N 48.25 29.00 40.27 15.00
312 PRO CD 47.72 28.46 41.53 15.00
312 PRO CA 49.07 30.17 40.52 15.00
312 PRO CB 48.67 30.54 41.94 15.00
312 PRO CG 48.52 29.21 42.56 15.00
312 PRO C 50.56 29.86 40.47 15.00
312 PRO 0 50.97 28.70 40.58 15.00
313 LYS N 51.37 30.91 40.29 15.00
313 LYS CA 52.82 30.83 40.25 15.00
313 LYS CB 53.38 31.51 39.00 15.00
313 LYS CG 53.01 30.87 37.69 15.00
313 LYS CD 53.59 31.66 36.51 15.00
313 LYS CE 53.12 33.11 36.50 15.00
313 53.57 33.86 35.30 15.00
LYS
NZ
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313 LYS C 53.26 31.61 41.46 15.00
313 LYS O 53.37 32.84 41.41 15.00
314 MET N 53.37 30.92 42.58 15.00
314 MET CA 53.80 31.54 43.82 15.00
314 MET CB 53.53 30.56 44.97 15.00
314 MET CG 54.01 31.02 46.31 15.00
324 MET SD 53.49 29.88 47.59 15.00
314 MET CE 53.68 30.97 49.05 15.00
314 MET C 55.29 31.91 43.73 15.00
314 MET OT1 55.71 32.88 44.41 15.00
314 MET OT2 56.02 31.24 42.96 15.00
502 HOH OH2 38.18 18.74 57.83 15.00
503 HOH OH2 41.01 35.17 62.98 15.00
505 HOH OH2 44.29 24.31 61.53 15.00
509 HOH OH2 45.95 46.05 53.57 15.00
510 HOH OH2 30.32 -1.58 47.62 15.00
511 HOH OH2 37.82 6.12 45.60 15.00
512 HOH OH2 48.03 7.94 27.32 15.00
514 HOH OH2 30.21 15.76 57.13 15.00
515 HOH OH2 45.27 21.35 49.85 15.00
516 HOH OH2 46.41 46.23 50.69 25.00
517 HOH OH2 53.91 41.75 43.17 15.00
518 HOH OH2 20.50 21.52 37.73 15.00
519 HOH OH2 21.73 20.43 33.70 15.00
520 HOH OH2 27.61 12.32 27.20 15.00
521 HOH OH2 47.60 46.15 47.79 15.00
522 HOH OH2 61.85 26.15 53.68 15.00
523 HOH OH2 43.95 24.65 64.43 15.00
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Table TI
Provides the distances between interresidue atoms that are
within 5 angstroms apart in the active site of the procathepsin
K according to this invention.
Atom 1 Atom 2 Distance Atom 1 Atom 2 Distance
75N 121 0 4.995 75 CA 118 NE2 3.873
75CA 121 O 4.355 75 CA 118 CD 4.497
75CA 283 NE1 4.706 75 CA 118 OE1 4.948
75CA 119 O 4.987 75 C 118 NE2 3.466
75C 118 CD 4.018 75 C 118 OE1 4.145
75C 283 NE1 4.154 75 C 121 O 4.560
75C 283 CZ2 4.800 75 C 283 CE2 4.835
75C 261 CE1 4.979 75 O 283 NE1 3.017
75O 118 OE1 3.398 75 O 118 NE2 3.416
75O 118 CD 3.559 75 O 283 CE2 3.721
75O 283 CZ2 3.785 75 O 261 CE1 3.966
750 283 CD1 4.133 75 O 261 ND1 4.354
75O 118 CG 4.672 75 O 261 NE2 4.856
75CB 283 NE1 3.971 75 CB 118 NE2 4.521
75CB 283 CE2 4.555 75 CB 119 0 4.622
75CB 283 CD1 4.636 75 CB 118 CD 4.756
75CB 283 CZ2 4.817 75 CG 119 O 3.992
75CG 119 C 4.854 75 CG 283 NE1 4.957
75SD 119 O 4.544 75 SD 283 NE1 4.903
75SD 283 CD1 4.988 75 CE 119 0 3.405
75CE 119 C 4.162 75 CE 283 CD1 4.953
76N 118 NE2 3.957 76 N 121 0 4.698
76N 118 CD 4.726 76 N 118 OE1 4.792
76N 122 CA 4.921 76 CA 261 ND1 4.016
76CA 124 CB 4.124 76 CA 118 NE2 4.359
76CA 260 0 4.376 76 CA 124 SG 4.392
76CA 261 CE1 4.402 76 CA 118 OE1 4.758
76CA 118 CD 4.992 76 C 124 SG 3.895
76C 124 CB 3.957 76 C 122 CA 4.219
76C 260 O 4.588 76 C 118 NE2 4.643
76C 122 C 4.746 76 C 124 N 4.779
76C 261 ND1 4.945 76 0 122 CA 2.996
760 122 C 3.572 76 O 122 O 3.992
76O 124 CB 3.996 76 O 118 NE2 4.014
76O 121 O 4.109 76 O 124 N 4.132
760 122 N 4.146 76 O 123 N 4.207
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76 124 SG 4.211 76 121 C 4.541
O 0
76 124 CA 4.711 76 CB 260 O 3.250
O
76 CB 261 ND1 3.886 76 CB 260 C 4.410
76 CB 261 CE1 4.599 76 CB 261 CG 4.680
76 CB 261 CA 4.690 76 CB 261 CB 4.819
76 CB 124 SG 4.909 76 OG1 260 O 3.483
76 OG1260 C 4.657 76 OG1 260 CB 4.921
76 CG2261 ND1 3.842 76 CG2 260 O 3.973
76 CG2283 CZ2 4.184 76 CG2 261 CE1 4.378
76 CG2261 CG 4.410 76 CG2 261 CB 4.672
76 CG2260 OD1 4.817 76 CG2 260 C 4.945
77 N 124 SG 3.789 77 N 260 O 3.832
77 N 124 CB 4.431 77 N 260 C 4.971
77 N 122 CA 4.995 77 CA 124 SG 4.104
77 CA 164 CA 4.268 77 CA 260 O 4.740
77 CA 122 CA 4.753 77 CA 122 O 4.869
77 CA 164 N 4.965 77 CA 124 CB 4.989
77 C 124 SG 3.712 77 C 164 CA 4.149
77 C 165 N 4.290 77 C 165 O 4.329
77 C 260 0 4.727 77 C 164 C 4.784
77 O 165 N 3.086 77 O 164 CA 3.304
77 O 165 0 3.351 77 O 164 C 3.677
77 O 165 C 4.096 77 O 124 SG 4.131
77 0 165 CA 4.156 77 O 125 CD1 4.373
77 O 164 N 4.519 77 0 125 CB 4.682
77 0 122 O 4.772 77 O 125 CG 4.846
77 O 164 O 4.878 77 O 125 N 4.998
78 N 124 SG 3.624 78 N 260 0 3.772
78 N 260 C 4.354 78 N 165 O 4.525
78 N 260 CA 4.893 78 CA 165 O 3.675
78 CA 124 SG 4.038 78 CA 260 O 4.469
78 CA 260 C 4.694 78 CA 165 C 4.858
78 C 165 O 3.470 78 C 165 C 4.671
78 C 165 N 4.812 78 O 165 O 4.549
78 O 260 CA 4.953 78 CB 260 C 3.787
78 CB 260 0 3.940 78 CB 261 N 4.054
78 CB 260 CA 4.128 78 CB 124 SG 4.281
78 CB 261 C 4.364 78 CB 261 CA 4.454
78 CB 261 0 4.473 78 CB 260 N 4.727
78 CB 262 4.729 78 CB 165 0 4.763
N
78 CG 166 4.339 78 233 CB 4.450
CD2 CG
78 CG 165 4.601 78 166 CE2 4.656
0 CG
78 261 4.703 78 4.930
CG O CG
260
C
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78CG 261 C 4.931 78 CG 261 N 4.956
78CD1 167 SD 3.767 78 CD1 166 CD2 3.914
78CD1 262 CB 4.020 78 CD1 167 CE 4.083
78CD1 233 CB 4.196 78 CD1 166 CE2 4.214
78CD1 165 O 4.482 78 CD1 261 O 4.510
78CD1 262 CA 4.616 78 CD1 261 C 4.720
78CD1 262 N 4.747 78 CD1 124 SG 4.832
78CD1 166 CA 4.850 78 CD1 166 CG 4.853
78CD2 233 CB 3.462 78 CD2 261 O 4.404
78CD2 260 N 4.528 78 CD2 233 CA 4.611
78CD2 260 CA 4.717 78 CD2 261 N 4.737
78CD2 166 CE2 4.755 78 CD2 166 CD2 4.849
78CD2 260 C 4.851 78 CD2 261 C 4.961
79N 165 O 2.577 79 N 165 C 3.666
79N 165 N 3.694 79 N 165 CA 4.214
79N 164 C 4.711 79 N 166 N 4.779
79N 164 CA 4.792 79 CA 165 O 3.276
79CA 165 N 4.098 79 CA 165 C 4.120
79CA 165 CA 4.486 79 C 165 O 3.582
79C 165 C 4.428 79 C 166 CD2 4.589
79C 166 CB 4.702 79 O 165 O 3.305
79O 166 CB 3.633 79 O 166 CD2 3.799
790 165 C 3.919 79 O 166 CG 4.132
790 166 CA 4.358 79 O 166 N 4.424
790 165 CA 4.788 79 O 166 CE2 4.878
79CB 165 O 3.121 79 CB 165 N 3.244
79CB 165 CA 3.495 79 CB 165 C 3.524
79CB 164 C 4.048 79 CB 164 CA 4.500
79CB 166 N 4.607 79 CB 164 O 4.838
79CG 165 N 4.216 79 CG 165 CA 4.486
79CG 165 0 4.575 79 CG 164 C 4.673
79CG 165 C 4.798 79 CD 165 N 4.409
79CD 164 CA 4.436 79 CD 164 C 4.493
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Table III
Provides the bond angles between interresidue atoms that
are within 5 l~ngstroms apart in the active site region of,
procathepsin K according to this invention.
Atom 1 Atom 2 Atom 3 Angle Atom 1 Atom 2 Atom 3 Angle
118NE2 75CA 1220 36.06 118NE2 75CA 118CD 16.04
118NE2 75CA 283NE1 66.79 118NE2 75CA 1180E1 25.84
118NE2 75CA 1190 58.31 1210 75CA 118CD 50.49
1210 75CA 1180E1 61.89 1210 75CA 1190 58.01
118CD 75CA 283NE1 51.41 118CD 75CA 1180E1 13.90
118CD 75CA 1190 54.73 283NE1 75CA 1180E1 41.86
283NE1 75CA 1190 75.92 1180E1 75CA 1190 66.13
118NE2 75C 118CD 18.51 118NE2 75C 1180E1 32.60
118NE2 75C 283NE1 76.97 1~.8NE2 75C 1210 34.31
118NE2 75C 283CE2 91.87 118NE2 75C 261CE1 75.88
118CD 75C 1180E1 17.20 118CD 75C 283NE1 58.52
118CD 75C 1210 51.78 118CD 75C 283CZ2 87.90
118CD 75C 283CE2 73.37 118CD 75C 261CE1 63.49
1180E1 75C 283NE1 49.23 1180E1 75C 1210 66.91
1180E1 75C 283CZ2 74.96 1180E1 75C 283CE2 62.58
1180E1 75C 261CE1 46.33 283NE1 75C 283CZ2 31.41
283NE1 75C 283CE2 15.20 283NE1 75C 261CE1 48.42
.
283CZ2 75C 283CE2 16.61 283CZ2 75C 261CE1 48.64
283CE2 75C 261CE1 49.08 283NE1 750 1180E1 64.89
283NE1 750 118NE2 95.58 283NE1 750 118CD 74.33
283NE1 750 283CE2 20.15 283NE1 750 283CZ2 41.26
283NE1 750 261CE1 64.66 283NE1 750 283CD1 12.81
283NE1 750 261ND1 79.83 283NE1 750 118CG 66.93
283NE1 750 261NE2 53.74 1180E1 750 118NE2 38.26
1180E1 750 118CD 20.14 1180E1 750 283CE2 82.53
1180E1 750 283CZ2 98.99 1180E1 750 261CE1 59.18
1180E1 750 283CD1 57.60 1180E1 750 261ND1 72.46
1180E1 750 118CG 29.49 1180E1 750 261NE2 62.96
118NE2 750 118CD 21.71 118NE2 750 261CE1 92.08
118NE2 750 283CD1 84.38 118NE2 750 261ND1 99.59
118NE2 750 118CG 29.92 118NE2 750 261NE2 99.17
118CD 750 283CE2 94.09 118CD 750 261CE1 79.13
118CD 750 283CD1 63.81 118CD 750 261ND1 91.35
118CD 750 118CG 14.54 118CD 750 261NE2 83.02
283CE2 750 283CZ2 21.35 2$3CE2 750 261CE1 63.98
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283CE2 750 283CD1 32.37 283CE2 750 261ND1 75.0
0
283CE2 750 218CG 87.07 283CE2 750 261NE2 _
51.36
283CZ2 750 261CE1 62.61 283CZ2 750 283CD1 53.70
283CZ2 750 261ND1 67.57 283CZ2 750 261NE2 50.53
261CE1 750 283CD1 71.28 261CE1 750 261ND1 17.46
261CE1 750 118CG 87.41 261CE1 750 261NE2 12.73
283CD1 750 261ND1 87.81 283CD1 750 118CG 55.01
283CD1 750 261NE2 61.92 261ND1 750 261NE2 26.12
118CG 750 261NE2 88.52 283NE1 75CB 118NE2 68.03
283NE1 75CB 283CE2 16.72 283NE1 75CB 1190 87.60
283NE1 75CB 283CD1 15.99 283NE1 75CB 118CD 53.59
283NE1 75CB 283CZ2 31.23 118NE2 75CB 283CE2 83.45
118NE2 75CB 1190 57.88 118NE2 75CB 283CD1 67.64
118NE2 75CB 118CD 16.12 118NE2 75CB 283CZ2 90.96
283CE2 75CB 283CD1 28.00 283CE2 75CB 118CD 69.80
283CE2 75CB 283CZ2 16.78 1190 75CB 283CD1 73.70
1190 75CB 118CD 55.68 283CD1 75CB 118CD 51.68
283CD1 75CB 283CZ2 44.58 118CD 75CB 283CZ2 79.88
1190 75CG 119C 11.39 1190 75CG 283NE1 82.86
119C 75CG 283NE1 94.25 2190 75SD 283NE1 78.22
1190 75SD 283CD1 71.07 283NE1 75SD 283CD1 15.84
1190 75CE 119C 14.76 1190 75CE 283CD1 80.96
119C 75CE 283CD1 94.94 118NE2 76N 1210 33.42
118NE2 76N 118CD 14.27 118NE2 76N 1180E1 27.52
118NE2 76N 122CA 56.37 1210 76N 118CD 47.26
1210 76N 1180E1 60.91 1210 76N 122CA 32.17
118CD 76N 1180E1 14.79 118CD 76N 122CA 70.12
1180E1 76N 122CA 79.60 261ND1 76CA 124CB 54.84
261ND1 76CA 118NE2 90.77 261ND1 76CA 2600 61.33
261ND1 76CA 124SG 58.56 261ND1 76CA 261CE1 17.26
261ND1 76CA 1180E1 63.29 261ND1 76CA 118CD 77.49
124CB 76CA 118NE2 62.81 124CB 76CA 2600 85.35
124CB 76CA 124SG 24.21 124CB 76CA 261CE1 53.22
124CB 76CA 1180E1 51.81 124CB 76CA 118CD 58.94
118NE2 76CA 124SG 85.37 118NE2 76CA 261CE1 74.91
118NE2 76CA 1180E1 27.92 118NE2 76CA 118CD 14.28
2600 76CA 124SG 65.30 2600 76CA 261CE1 78.56
124SG 76CA 261CE1 64.93 124SG 76CA 1180E1 75.85
124SG 76CA 118CD 83.00 261CE1 76CA 1180E1 47.04
261CE1,76CA 118CD 61.13 1180E1 76CA 118CD 14.20
124SG 76C 124CB 26.57 124SG 76C 122CA 99.52
.
124SG 76C 2600 67.22 124SG 76C 118NE2 87.57
124SG 76C 122C 81.40 124SG 76C 124N 48.42
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124SG 76C 261ND1 54.07 124CB 76C 122CA 87.7_4
124CB 76C 2600 84.53 124CB 76C 118NE2 61.31
124CB 76C 122C 70.61 124CB 76C 124N 31.25
124CB 76C 261ND1 48.28 122CA 76C 118NE2 57.49
122CA 76C 122C 18.19 122CA 76C 124N 56.49
2600 76C 261ND1 53.35 118NE2 76C 122C 55.01
118NE2 76C 124N 47.65 118NE2 76C 261ND1 76.88
122C 76C 124N 39.55 124N 76C 261ND1 77.02
122CA 760 122C 24.62 122CA 760 1220 36.26
122CA 760 118NE2 73.62 122CA 760 1210 40.55
122CA 760 124N 71.96 122CA 760 122N 14.30
122CA 760 123N 34.47 122CA 760 121C 28.56
122CA 760 124CA 89.43 122C 760 1220 17.57
122C 760 124CB 83.93 222C 760 118NE2 69.46
122C 760 1210 48.92 122C 760 124N 48.79
122C 760 122N 36.55 122C 760 123N 17.28
122C 760 124SG 93.20 122C 760 121C 44.07
122C 760 124CA 65.97 1220 760 124CB 80.66
1220 760 118NE2 84.24 1220 760 1210 66.48
1220 760 124N 49.34 1220 760 122N 50.21
1220 760 123N 31.60 1220 760 124SG 83.04
1220 760 121C 60.61 1220 760 124CA 64.22
124CB 760 118NE2 67.06 124CB 760 1210 96.67
124CB 760 124N 35.52 124CB 760 123N 73.72
124CB 760 124SG 25.24 124CB 760 124CA 18.06
118NE2 760 1210 37.13 218NE2 760 124N 55.72
118NE2 760 122N 67.51 118NE2 760 123N 52.88
118NE2 760 124SG 92.30 118NE2 760 121C 52.23
118NE2 760 124CA 60.34 1210 760 124N 69.55
1210 760 122N 31.11 1210 760 123N 40.00
1210 760 121C 15.12 1210 760 124CA 83.05
124N 760 122N 79.70 124N 760 123N 38.53
124N 760 124SG 51.84 124N 760 121C 76.98
124N 760 124CA 17.47 122N 760 123N 41.20
122N 760 121C 16.68 122N 760 124CA 96.89
123N 760 124SG 88.96 123N 760 121C 41.29
123N 760 124CA 55.85 124SG 760 124CA 36.87
121C 760 124CA 92.58 2600 76CB 261ND1 73.33
2600 76CB 260C 5.97 2600 76CB 261CE1 88.42
2600 76CB 261CG 64.24 2600 76CB 261CA 33.95
2600 76CB 261CB 47.27 2600 76CB 124SG 67.36
261ND1 76CB 260C 69.61 261ND1 76CB 261CE1 15.10
261ND1 76CB 261CG 15.13 261ND1 76CB 261CA 39.38
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261ND1 76CB 261CB 31.52 261ND1 76CB 124SG 54.44
260C 76CB 261CE1 84.69 260C 76CB 261CG 59.58
260C 76CB 261CA 30.41 260C 76CB 261CB 42.17
260C 76CB 124SG 69.37 261CE1 76CB 261CG 27.44
261CE1 76CB 261CA 54.48 261CE1 76CB 261CB 45.34
261CE1 76CB 124SG 59.43 261CG 76CB 261CA 31.56
261CG 76CB 261CB 18.14 261CG 76CB 124SG 63.87
261CA 76CB 261CB 18.49 261CA 76CB 124SG 54.00
261CB 76CB 124SG 66.72 2600 76061 260C 5.04
2600 76061 260CB 34.36 260C 76061 260CB 29.73
261ND1 76CG2 2600 66.59 262ND1 76CG2 283CZ2 68.93
261ND1 76CG2 261CE1 16.92 261ND1 76CG2 261CG 17.51
261ND1 76CG2 261CB 32.75 261ND1 76CG2 2600D1 81.35
261ND1 76CG2 260C 64.09 2600 76CG2 261CE1 83.30
2600 76CG2 261CG 62.86 2600 76CG2 261CB 47.50
2600 76CG2 2600D1 42.82 2600 76CG2 260C 9.65
283CZ2 76CG2 261CE1 56.11 283CZ2 76CG2 261CG ,66.23
283CZ2 76CG2 261CB 80.03 261CE1 76CG2 261CG 29.03
261CE1 76CG2 261CB 47.23 261CE1 76CG2 2600D1 95.65
261CE1 76CG2 260C 81.00 261CG 76CG2 261CB 18.77
261CG 76CG2 2600D1 66.64 261CG 76CG2 260C 57.48
261CB 76CG2 2600D1 48.72 261CB 76CG2 260C 40.55
2600D1 76CG2 260C 34.76 124SG 77N 2600 76.74
124SG 77N 124CB 23.76 124SG 77N 260C 73.38
124SG 77N 122CA 88.63 2600 77N 124CB 88.17
2600 77N 260C 5.96 124CB 77N 260C 86.75
124CB 77N 122CA 73.67 124SG 77CA 2600 64.21
124SG 77CA 122CA 88.49 124SG 77CA 1220 74.09
124SG 77CA 124CB 20.03 164CA 77CA 122CA 70.18
164CA 77CA 1220 56.41 164CA 77CA 164N 15.89
2600 77CA 124CB 72,64 122CA 77CA 1220 28.50
122CA 77CA 164N 57.26 122CA 77CA 124CB 71.13
1220 77CA 164N 50.18 1220 77CA 124CB 63.25
124SG 77C 1650 94.31 124SG 77C 2600 66.95
164CA 77C 165N 32.95 164CA 77C 1650 69.26
164CA 77C 164C 17.70 165N 77C 1650 36.54
165N 77C 164C 15.64 1650 77C 164C 52.18
165N 770 164CA 43.90 165N 770 1650 49.43
165N 770 164C 20.33 165N 770 165C 36.57
165N 770 165CA 15.71 165N 770 125CD1 57.18
165N 770 164N 53.60 165N 770 125CB 67.06
165N 770 1220 78,73 165N 770 125CG 56.08
265N 770 1640 20.42 165N 770 125N 92.35
94
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164CA 770 1650 92.82 164CA 770 164C 24.23
164CA 770 165C 80.47 164CA 770 165CA 59.42
164CA 770 125CD1 69.56 164CA 770 164N 11.76
164CA 770 125CB 97.27 164CA 770 1220 62.09
164CA 770 125CG 79.85 164CA 770 2640 26.06
1650 770 164C 69.76 1650 770 165C 15.26
1650 770 165CA 35.08 1650 770 125CD1 73.77
1650 770 125CB 53.64 1650 770 125CG 60.06
1650 770 1640 69.49 1650 770 125N 81.29
164C 770 165C 56.55 164C 770 165CA 35.40
164C 770 125CD1 56.97 164C 770 164N 33.30
164C 770 125CB 77.67 164C 770 1220 66.26
164C 770 125CG 62.33 164C 770 1640 3.99
164C 770 125N 96.65 165C 770 165CA 21.15
165C 770 125CD1 61.03 165C 770 164N 89.72
165C 770 125CB 47.59 165C 770 1220 96.34
165C 770 125CG 49.12 165C 770 1640 55.72
165C 770 125N 76.96 124SG 770 125CD1 77.46
124SG 770 125CB 64.58 124SG 770 1220 74.93
124SG 770 125CG 75.13 124SG 770 125N 38.93
165CA 770 125CD1 54.72 165CA 770 164N 68.58
165CA 770 125CB 55.56 165CA 770 1220 84.07
165CA 770 125CG 48.67 165CA 770 1640 34.63
165CA 770 125N 83.50 125CD1 770 164N 67.76
125CD1 770 125CB 32.79 125CD1 770 1220 37.02
125CD1 770 125CG 15.92 125CD1 770 1640 52.99
125CD1 770 125N 39.98 164N 770 125CB 98.50
164N 770 1220 53.25 164N 770 125CG 80.38
164N 770 1640 34.03 125CB 770 1220 65.89
125CB 770 125CG 18.18 125CB 770 1640 74.21
125CB 770 125N 29.43 1220 770 125CG 52.30
1220 770 1640 63.19 1220 770 125N 54.39
125CG 770 1640 58.58 125CG 770 125N 36.28
1640 770 125N 92.69 124SG 78N 2600 79.51
124SG 78N 260C 83.08 124SG 78N 1650 92.32
2600 78N 260C 15.29 2600 78N 260CA 28.16
260C 78N 260CA 17.65 1650 78CA 224SG 99.93
1650 78CA 165C 4.78 124SG 78CA 2600 67.35
124SG 78CA 260C 74.63 124SG 78CA 165C 95.67
2600 78CA 260C 15.11 1650 78C 165C 4.04
1650 78C 165N 33.36 165C 78C 165N 29.89
260C 78CB 2600 18.11 260C 78CB 261N 19.07
260C 78CB 260CA 21.52 260C 78CB 124SG 82.24
CA 02301201 2000-02-04
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260C 78CB 261C 50.82 260C 78CB 261CA 32.63
260C 78CB 2610 55.55 260C 78CB 260N 32.43
260C 78CB 262N 63.04 2600 78CB 261N 32.35
2600 78CB 260CA 34.09 2600 78CB 124SG 70.13
2600 78CB 261C 56.71 2600 78CB 261CA 36.83
2600 78CB 2610 66.27 2600 78CB 260N 48.80
2600 78CB 262N 64.33 261N 78CB 260CA 34.77
261N 78CB 124SG 77.85 261N 78CB 261C 33.28
261N 78CB 261CA 18.95 261N 78CB 2610 36.48
261N 78CB 260N 36.74 261N 78CB 262N 47.56
260CA 78CB 2610 67.94 260CA 78CB 261CA 52.32
260CA 78CB 2610 67.73 260CA 78CB 260N 17.14
260CA 78CB 262N 82.21 224SG 78CB 261C 58.84
124SG 78CB 261CA 59.89 124SG 78CB 2610 73.12
124SG 78CB 262N 44.93 124SG 78CB 1650 81.43
261C 78CB 261CA 19.89 261C 78CB 2610 I5.94
261C 78CB 260N 66.21 261C 78CB 262N 16.25
261CA 78CB 2610 31.13 261CA 78CB 260N 55.67
261CA 78CB 262N 30.41 2610 78CB 260N 61.05
2610 78CB 262N 28.30 260N 78CB 262N 82.18
166CD2 7$CG 233CB 92.45 166CD2 78CG 1650 59.87
166CD2 78CG 166CE2 17.29 233CB 78CG 166CE2 76.12
233CB 78CG 2610 46.52 233CB 78CG 260C 84.76
233CB 78CG 261C 60.56 233CB 78CG 261N 71.12
1650 78CG 166CE2 77.08 2610 78CG 260C 47.65
2610 78CG 261C 24.42 2610 78CG 261N 32.31
260C 78CG 261C 42.03 260C 78CG 261N 15.40
261C 78CG 261N 28.44 167SD 78CD1 166CD2 75.55
167SD 78CD1 262CB 57.53 167SD 78CD1 167CE 25.64
167SD 78CD1 233CB 89.72 167SD 78CD1 166CE2 73.72
167SD 78CDI 1650 93.53 167SD 78CD1 2610 98.39
167SD 78CD1 262CA 68.67 167SD 78CD1 261C 98.69
167SD 78CD1 262N 85.99 167SD 78CD1 124SG 90.32
267SD 78CD1 166CA 64.21 167SD 78CD1 166CG 67.34
166CD2 78CD1 167CE 65.10 166CD2 78CD1 166CE2 I9.20
166CD2 78CD1 1650 63.87 166CD2 78CD1 166CA 42.23
166CD2 78CD1 166CG 13.29 262CB 78CD1 167CE 74.75
262CB 78CD1 233CB 86.41 262CB 78CD1 2610 54.29
262CB 78CD1 262CA 18.46 262CB 78CD1 261C 46.32
262CB 78CD1 262N 30.66 262CB 78CD1 224SG 43.36
.
I67CE 78CD1 233CB 70.78 167CE 78CD1 166CE2 55.94
167CE 78CD1 2610 97.46 167CE 78CD1 262CA 79.31
167CE 78CD1 262N 96.89 167CE 78CD1 166CA 73.32
96
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167CE 78CD1 166CG 62.41 233CB 78CD1 166CE2 83.80
233CB 78CD1 2610 49.01 233CB 78CD1 262CA 70.73
233CB 78CD1 261C 64.04 233CB 78CD1 262N 73.78
166CE2 78CD1 1650 83.05 166CE2 78CD1 166CA 59.39
166CE2 78CD1 166CG 29.73 1650 78CD1 124SG 78.68
1650 78CD1 166CA 34.26 1650 78CD1 166CG 56.08
2610 78CD1 262CA 36.15 2610 78CD1 261C 15.09
2610 78CD1 262N 28.16 2610 78CD1 124SG 67.79
262CA 78CD1 261C 30.54 262CA 78CD1 262N 17.84
262CA 78CD1 124SG 50.40 261C 78CD1 262N 16.21
261C 78CD1 124SG 52.78 262N 78CD1 124SG 42.44
124SG 78CD1 166CA 96.43 166CA 78CD1 166CG 29.75
233CB 78CD2 2610 53.21 233CB 78CD2 260N 92.15
233CB 78CD2 233CA 14.15 233CB 78CD2 261N 82.42
233CB 78CD2 166CE2 84.68 233CB 78CD2 166CD2 98.20
233CB 78CD2 260C 97.86 233CB 78CD2 261C 65.86
2610 78CD2 260N 63.15 2610 78CD2 233CA 39.06
2610 78CD2 260CA 63.42 2610 78CD2 261N 34.09
2610 78CD2 260C 49.52 2610 78CD2 261C 13.47
260N 78CD2 233CA 83.98 260N 78CD2 260CA 17.85
260N 78CD2 261N 35.61 260N 78CD2 260C 31.22
260N 78CD2 261C 63.05 233CA 78CD2 260CA 93.30
233CA 78CD2 261N 69.20 233CA 78CD2 166CE2 94.77
233CA 78CD2 260C 84.90 233CA 78CD2 261C 51.75
260CA 78CD2 261N 29.99 260CA 78CD2 260C 18.16
260CA 78CD2 261C 58.71 261N 78CD2 260C 15.83
261N 78CD2 261C 28.89 166CE2 78CD2 166CD2 16.58
260C 78CD2 261C 42.24 1650 79N 165C 10.81
1650 79N 165N 47.00 1650 79N 165CA 30.61
1650 79N 164C 58.72 1650 79N 166N 7.73
1650 79N 164CA 75.06 165C 79N 165N 38.87
165C 79N 165CA 20.71 165C 79N 164C 50.56
165C 79N 166N 9.97 165C 79N 164CA 67.94
165N 79N 165CA 19.76 165N 79N 164C 11.75
165N 79N 166N 48.84 165N 79N 164CA 29.34
165CA 79N 164C 30.89 165CA 79N 166N 30.45
165CA 79N 164CA 48.99 164C 79N 166N 60.54
164C 79N 164CA 18.26 166N 79N 164CA 77.85
1650 79CA 165N 41.13 2650 79CA 165C 14.08
1650 79CA 165CA 31.17 165N 79CA 165C 34.67
165N 79CA 165CA 18.76 165C 79CA 165CA 19.70
1650 79C 165C 12.94 1650 79C 166CD2 64.92
1650 79C 166CB 46.02 165C 79C 166CD2 65.90
97
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165C 79C 166CB 40.97 166CD2 79C 166CB 31.09
1650 790 166CB 58.43 1650 790 166CD2 77.59
1650 790 165C 17.10 1650 790 166CG 71.57
1650 790 166CA 39.48 1650 790 166N 29.63
1650 790 165CA 27.24 1650 790 166CE2 87.44
166CB 790 166CD2 39.11 166CB 790 165C 50.05
166CB 790 166CG 20.86 166CB 790 166CA 19.18
166CB 790 166N 33.28 166CB 790 165CA 62.88
166CB 790 166CE2 50.06 166CD2 790 165C 78.93
166CD2 790 166CG 19.43 166CD2 790 166CA 46.73
166CD2 790 166N 65.76 166CD2 790 165CA 95.02
166CD2 790 166CE2 11.63 165C 790 166CG 67.58
165C 790 166CA 33.62 165C 790 166N 16.99
165C 790 165CA 16.49 165C 790 166CE2 90.16
166CG 790 166CA 33.98 166CG 790 166N 51.93
166CG 790 165CA 82.12 166CG 790 166CE2 29.53
166CA 790 166N 19.03 166CA 790 165CA 48.76
166CA 790 166CE2 58.34 166N 790 165CA 30.19
166N 790 166CE2 77.37 1650 79CB 165N 50.20
1650 79CB 165CA 41.86 1650 79CB 165C 20.25
1650 79CB 164C 66.94 1650 79CB 164CA 76.08
1650 79CB 166N 25.81 1650 79CB 1640 71.51
165N 79CB 165CA 24.50 165N 79CB 165C 42.17
165N 79CB 164C 16.78 165N 79CB 164CA 31.00
165N 79CB 166N 51.76 165N 79CB 1640 23.32
165CA 79CB 165C 24.94 165CA 79CB 164C 36.59
165CA 79CB 164CA 54.55 165CA 79CB 166N 31.14
165CA 79CB 1640 34.36 165C 79CB 164C 58.02
165C 79CB 164CA 72.65 165C 79CB 166N 10.99
165C 79CB 1640 58.61 164C 79CB 164CA 19.44
164C 79CB 166N 66.75 164C 79CB 1640 12.33
164CA 79CB 166N 82.64 164CA 79CB 1640 29.49
166N 79CB 1640 65.48 165N 79CG 165CA 18.86
165N 79CG 1650 35.50 165N 79CG 164C 16.15
165N 79CG 165C 30.67 165CA 79CG 1650 30.56
165CA 79CG 164C 30.62 165CA 79CG 165C 18.40
1650 79CG 164C 51.63 1650 79CG 165C 14.88
164C 79CG 165C 45.97 165N 79CD 164CA 31.44
165N 79CD 164C 17.13 164CA 79CD 164C 19.46
98
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Table IV
Table of the orthogonal three dimensional coordinates in
angstroms and B factors (~2) for procathepsin K active site
atoms and atoms of the propeptide in the region of the active
site.
Residue Atom X Y Z B
75 METN 25.19 25.27 46.93 15.00
75 METCA 26.49 24.70 47.29 25.00
75 METC 27.52 24.57 46.17 15.00
75 METO 28.31 23.63 46.18 15.00
75 METCB 26.30 23.34 47.96 15.00
75 METCG 25.56 23.38 49.25 15.00
75 METSD 25.38 21.74 49.87 15.00
75 METCE 26.23 21.91 51.38 15.00
76 THRN 27.50 25.45 45.19 15.00
76 THRCA 28.48 25.45 44.12 15.00
76 THRC 28.74 26.93 43.86 15.00
76 THR0 28.92 27.68 44.82 15.00
76 THRCB 27.97 24.75 42.84 15.00
76 THROG1 26.90 25.50 42.28 15.00
76 THRCG2 27.50 23.34 43.14 15.00
77 GLYN 28.75 27.36 42.60 15.00
77 GLYCA 28.96 28.77 42.33 15.00
77 GLYC 30.07 29.15 41.36 15.00
77 GLY0 30.68 30.20 41.51 15.00
78 LEUN 30.32 28.33 40.35 15.00
78 LEUCA 31.38 28.66 39.42 15.00
78 LEUC 30.86 29.69 38.47 15.00
78 LEUO 30.29 29.33 37.44 15.00
78 LEUCB 31.85 27.45 38.61 15.00
78 LEUCG 33.00 27.84 37.67 15.00
78 LEUCD1 34.30 27.78 38.44 15.00
78 L~UCD2 33.07 26.95 36.46 15.00
79 LYSN 31.00 30.96 38.81 15.00
79 LYSCA 30.57 32.02 37.93 15.00
79 LYSC 31.52 31.90 36.75 15.00
99
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79 LYS O 32.58 32.52 .36.75 15.00
79 LYS CB 30.77 33.37 38.60 15.00
79 LYS CG 29.87 34.48 38.12 15.00
79 LYS CD 28.54 34.41 38.82 15.00
79 LYS CE 27.78 35.74 38.71 15.00
79 LYS NZ 28.35 36.82 39.58 15.00
118GLN N 32.65 23.81 52.55 15.00
118GLN CA 32.21 25.12 52.08 15.00
118GLN C 30.86 25.59 52.59 15.00
118GLN O 30.32 26.57 52.09 15.00
118GLN CB 32.17 25.14 50.55 15.00
118GLN CG 30.98 24.41 49.94 15.00
118GLN CD 30.83 24.72 48.46 15.00
118GLN OE1 31.38 24.01 47.61 15.00
118GLN NE2 30.11 25.77 48.14 15.00
119GLY N 30.32 24.91 .53.58 15.00
119GLY CA 29.02 25.31 54.10 15.00
119GLY C 28.03 25.31 52.96 15.00
119GLY 0 28.21 24.61 51.97 15.00
121CYS N 27.33 27.97 51.04 15.00
121CYS CA 27.63 29.26 50.43 15.00
121CYS C 28.38 29.01 49.13 15.00
121CYS O 28.91 27.93 48.92 15.00
121CYS CB 28.43 30.13 51.42 15.00
121CYS SG 29.68 31.27 50.76 15.00
122GLY N 28.41 29.98 48.24 15.00
122GLY CA 29.08 29.76 46.98 15.00
122GLY C 30.58 29.94 47.04 15.00
122GLY 0 31.13 30.69 46.24 15.00
123SER N 31.24 29.25 47.96 15.00
123SER CA 32.69 29.37 48.08 15.00
123SER C 33.44 28.36 47.23 15.00
123SER O 34.63 28.16 47.44 15.00
123SER CB 33.10 29.19 49.53 15.00
123SER OG 32.79 30.33 50.30 15.00
124CYS N 32.79 27.73 46.27 15.00
124CYS CA 33.49 26.74 45.45 15.00
124CYS C 34.71 27.36 44.77 15.00
100
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124 CYS O 35.80 26.79 44.79 15.00
224 CYS CB 32.55 26.04 44.46 15.00
124 CYS SG 32.47 26.70 42.78 15.00
125 TRP N 34.54 28.58 44.25 15.00
125 TRP CA 35.62 29.31 43.58 15.00
125 TRP C 36.72 29.59 '44.60 15.00
125 TRP O 37.89 29.34 44.34 15.00
125 TRP CB 35.11 30.63 42.97 15.00
125 TRP CG 34.60 31.64 43.98 15.00
125 TRP CD1 33.40 31.61 44.63 15.00
125 TRP CD2 35.30 32.77 44.49 15.00
125 TRP NE1 33.33 32.64 45.53 15.00
125 TRP CE2 34.48 33.37 45.47 15.00
125 TRP CE3 36.56 33.34 44.23 15.00
125 TRP CZ2 34.87 34.51 46.18 15.00
125 TRP CZ3 36.95 34.47 44.94 25.00
125 TRP CH2 36.10 35.04 45.90 15.00
126 ALA N 36.33 30.05 45.79 15.00
126 ALA CA 37.30 30.34 46.83 15.00
126 ALA C 37.90 29.09 47.45 15.00
126 ALA O 38.80. 29.19 48.28 15.00
126 ALA CB 36.68 31.21 47.90 15.00
127 PHE N 37.39 27.92 47.12 15.00
127 PHE CA 37.98 26.72 47.69 15.00
127 PHE C 38.98 26.09 46.74 15.00
127 PHE 0 40.05 25.67 47.16 15.00
127 PHE CB 36.92 25.73 48.16 15.00
127 PHE CG 36.48 25.95 49.59 15.00
127 PHE CD1 35.54 26.93 49.90 15.00
127 PHE CD2 .37.03 25.21 50.62 15.00
127 PHE CEI 35.17 27.16 51.21 15.00
227 PHE CE2 36.66 25.45 51.95 15.00
127 PHE CZ 35.72 26.42 52.24 15.00
128 SER N 38.66 26.08 45.46 15.00
128 SER CA 39.56 25.54 44.45 15.00
128 SER C 40.85 26.35 44.50 15.00
128 SER O 41.95 25.80 44.63 15.00
128 SER CB 38.91 25.64 43.07 15.00
101
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128 SER OG 39.60 24.88 42.09 15.00
129 SER N 40.72 27.68 44.44 15.00
129 SER CA 41.86 28.57 44.47 15.00
129 SER C 42.69 28.33 45.69 15.00
129 SER O 43.87 28.02 45.59 15.00
129 SER CB 41.40 30.01 44.43 25.00
129 SER OG 40.65 30.23 43.25 15.00
130 VAL N 42.07 28.41 46.85 15.00
130 VAL CA 42.79 28.18 48.10 15.00
130 VAL C 43.58 26.88 '48.04 15.00
130 VAL O 44.65 26.79 48.62 15.00
130 VAL CB 41.81 28.14 49.28 15.00
130 VAL CG1 42.27 27.18 50.35 15.00
130 VAL CG2 41.70 29.52 49.85 15.00
131 GLY N 43.08 25.91 47.29 15.00
131 GLY CA 43.74 24.61 47.18 15.00
131 GLY C 45.07 24.66 46.45 15.00
131 GLY O 46.06 24.12 46.94 15.00
149 PRO N 39.82 30.57 52.85 15.00
149 PRO CA 38.92 30.56 51.69 15.00
149 PRO C 38.04 31.80 51.73 15.00
149 PRO O 37.68 32.37 50.70 15.00
149 PRO CB 38.09 29.30 51.91 15.00
149 PRO CG 38.90 28.48 52.90 15.00
149 PRO CD 39.42 29.53 '53.81 15.00
153 VAL N 36.59 35.19 49.34 15.00
153 VAL CA 35.16 35.50 49.32 15.00
153 VAL C 34.78 36.95 49.62 15.00
153 VAL O 34.07 37.57 48.82 15.00
153 VAL CB 34.35 34.61 50.31 15.00
153 VAL CG1 32.85 34.96 50.23 15.00
153 VAL CG2 34.56 33.13 50.03 15.00
161 GLY N 31.35 36.59 45.80 15.00
161 GLY CA 31.46 35.98 47.11 15.00
161 GAY C 30.58 34.75 47.20 15.00
161 GLY O 30.57 33.93 46.28 15.00
162 CYS N 29.77 34.67 48.25 15.00
162 CYS CA 28.88 33.53 48.42 15.00
102
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162 CYS C 27.91 33.35 47.27 15.00
162 CYS O 27.27 32.31 47.16 15.00
162 CYS CB 28.11 33.63 49.74 15.00
162 CYS SG 29.06 33.14 51.22 15.00
164 GLY N 28.81 33.41 44.09 15.00
164 GLY CA 29.51 32.96 42.90 15.00
164 GLY C 30.80 33.70 42.64 15.00
164 GLY O 31.08 34.75 43.23 15.00
165 GLY N 31.60 33.14 41.73 15.00
165 GLY CA 32.87 33.73 41.37 15.00
165 GLY C 33.69 32.80 40.50 15.00
165 GLY O 33.15 31.84 39.94 15.00
166 TYR N 34.99 33.03 40.42 15.00
166 TYR CA 35.87 32.22 39.60 15.00
166 TYR C 37.28 32.18 .40.15 15.00
166 TYR 0 37.67 33.05 40.93 15.00
166 TYR CB 35.91 32.77 38.19 15.00
166 TYR CG 36.63 31.87 37.25 15.00
166 TYR CD1 37.97 32.08 36.95 15.00
166 TYR CD2 35.98 30.82 36.64 15.00
166 TYR CE1 38.64 31.27 36.05 15.00
166 TYR CE2 36.64 30.00 35.73 15.00
166 TYR CZ 37.96 30.23 35.43 15.00
166 TYR OH 38.59 29.45 34.50 15.00
167 MET N 38.08 31.24 39.66 15.00
267 MET CA 39.44 31.09 40.16 15.00
167 MET C 40.51 32.11 39.77 15.00
167 MET 0 41.15 32.69 40.66 15.00
167 MET CB 39.93 29.66 39.91 15.00
.
167 MET CG 39.21 28.59 40.73 15.00
167 MET SD 37.76 27.96 39.92 15.00
167 MET CE 38.38 27.66 38.30 15.00
188 TYR N 36.56 30.81 58.36 15.00
188 TYR CA 35.52 30.72 57.34 15.00
188 TYR C 34.23 30.68 58.14 15.00
188 TYR O 34.13 29.94 59.12 15.00
188 TYR CB 35.65 29.41 56.55 15.00
188 TYR CG 34.66 29.26 55.41 15.00
103
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188TYR CD1 34.74 30.08 54.27 15.00
188TYR CD2 33.66 28.28 55.45 15.00
188TYR CE1 33.85 29.92 53.20 15.00
188TYR CE2 32.77 28.12 54.38 15.00
188TYR CZ 32.88 28.94 53.26 15.00
188TYR OH 32.03 28.77 52.21 15.00
231SER N 43.74 23.58 39.76 15.00
231SER CA 42.33 23.89 39.96 15.00
231SER C 41.54 23.56 38.69 15.00
231SER O 42.02 23.82 37.58 15.00
231SER CB 42.17 25.38 40.29 15.00
231SER OG 42.60 25.66 41.61 15.00
232VAL N 40.35 22.96 38.84 15.00
232VAL CA 39.50 22.62 37.69 15.00
232VAL C 38.01 22.93 37.92 15.00
232VAL O 37.59 23.17 39.06 15.00
232VAL CB 39.65 21.13 37.25 15.00
232VAL CG1 41.00 20.91 36.59 15.00
232VAL CG2 39.47 20.20 38.43 15.00
233ALA N 37.24 22.97 .36.84 15.00
233ALA CA 35.81 23.26 36.88 15.00
233ALA C 35.12 22.07 36.25 15.00
233ALA O 35.63 21.51 35.29 15.00
233ALA CB 35.51 24.52 36.09 15.00
234ILE N 33.95 21.70 36.77 15.00
234ILE CA 33.20 20.54 36.28 15.00
234ILE C 31.69 20.74 36.29 15.00
234ILE O 31.19 21.79 36.70 15.00
234ILE CB 33.48 19.29 37.16 15.00
234ILE CG1 33.34 19.68 38.65 15.00
234ILE CG2 34.83 18.69 36.84 15.00
234ILE CD1 33.22 18.53 39.59 15.00
260ASN N 29.75 23.87 36.36 15.00
260ASN CA 29.17 24.51 37.52 15.00
260ASN C 29.82 24.27 38.87 15.00
260ASN O 29.30 24.73 39.88 15.00
260ASN CB 27.70 24.15 37.61 15.00
260ASN CG 27.47 22.66 37.68 15.00
I04
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
260ASN OD1 28.02 21.99 38.54 15.00
260ASN ND2 26.68 22.14 36.-75 15.00
261HIS N 30.91 23.52 38.92 15.00
261HIS CA 31.57 23.30 40.20 15.00
261HIS C 33.07 23.51 40.04 15.00
261HIS O 33.61 23.35 38.95 15.00
261HIS CB 31.25 21.91 40.75 15.00
261HIS CG 31.51 21.75 42.23 15.00
261HIS ND1 31.30 22.77 43.13 15.00
261HIS CD2 31.94 20.69 42.95 15,00
261HIS CE1 31.59 22.34 44.35 15.00
261HIS NE2 31.98 21.08 44.26 15.00
262ALA N 33.72 23.92 41.14 15.00
262ALA CA 35.15 24.16 41.18 15.00
262ALA C 35.81 23.18 42.16 15.00
262ALA 0 35.49 23.18 43.35 15.00
262ALA CB 35.41 25.58 41.62 15.00
263VAL N 36.75 22.39 41.66 15.00
263VAL CA 37.44 21.39 42.46 15.00
263VAL C 38.97 21.34 42.25 15.00
263VAL O 39.51 21.84 41.26 15.00
263VAL CB 36.85 19.99 42.18 15.00
263VAL CG1 35.47 19..89 42.73 15.00
263VAL CG2 36.82 19.71 ,40.69 15.00
264LEU N 39.66 20.70 43.18 15.00
264LEU CA 41.11 20.58 43.16 15.00
264LEU C 41.59 19.25 42.56 15.00
264LEU O 41.33 18.20 43.14 15.00
264LEU CB 41.65 20.69 44.59 15.00
264LEU CG 43.12 21.07 44.77 15.00
264LEU CD1 43.26 22.53 44.44 15.00
264LEU CD2 43.59 20.81 46.19 15.00
281ASN N 36.94 17.76 46.74 15.00
281ASN CA 35.74 18.57 46.76 15.00
281ASN C 35.73 19.48 47.98 15.00
281ASN O 36.70 19.50 48.74 15.00
281ASN CB 34.48 17.70 46.73 15.00
281ASN CG 33.38 18.32 45.91 15.00
105
CA 02301201 2000-02-04
WO 00/09653 PCT/US98/14634
281ASN OD1 32.82 19.36 46.27 15.00
281ASN ND2 33.09 17.72 44.77 15.00
282SER N 34.62 20.20 48.15 15.00
282SER CA 34.44 21.13 49.26 15.00
282SER C 33.19 20.80 50.05 15.00
282SER 0 32.67 21.65 50.76 15.00
282SER CB 34.30 22.55 48.70 15.00
282SER OG 34,88 22.65 47.41 15.00
283TRP N 32.71 19.57 49.93 15.00
283TRP CA 31.49 19.17 50.65 15.00
283TRP C 31.75 18.34 51.92 15.00
283TRP O 31.01 17.41 52.22 15.00
283TRP CB 30.50 18.46 49.71 15.00
283TRP CG 30.00 19.32 48.58 15.00
283TRP CD1 30.06 20.69 48.50 15.00
283TRP CD2 29.39 18.88 47.37 15.00
283TRP NE1 29.52 21.11 47.32 15.00
283TRP CE2 29.11 20.02 46.61 15.00
283TRP CE3 29.06 17.62 46.87 15.00
283TRP CZ2 28.51 19.94 45.35 15.00
283TRP CZ3 28.47 17.54 45.62 15.00
283TRP CH2 28.19 18.70 44.88 15.00
106