Note : Les descriptions sont présentées dans la langue officielle dans laquelle elles ont été soumises.
CA 02701378 2010-04-22
-1-
SEAWEED-DERIVED COSMETIC COMPOSITIONS
BACKGROUND OF THE INVENTION
1. Field of Invention
The present invention relates generally to a cosmetic composition and more
particularly to a cosmetic composition containing seaweed for application to
skin to
improve the appearance of the skin.
2. Description of Related Art
The skin is one of the largest organs in the body. Skin is comprised of three
main
layers: the epidermis, the dermis and subcutaneous layers. At the outermost
part of the
epidermis, a layer of dead cells forms what is known as a stratum comeum
layer. The
dermis is the middle layer of skin and is comprised of arrangements of
collagen fibres,
which surround many specialized cells and structures. The innermost layer of
the skin is
the subcutaneous layer, often called the sub-dermis. The subcutaneous layer is
comprised
largely of fat and connective tissue and houses larger blood vessels and
nerves. Elastin
may be found in all layers of the skin, but is most prominent in the dermis
layer.
The condition and appearance of the skin is a major concern to most people.
Enhancing the appearance of the skin is of significant interest for many
people. The
appearance of the skin can be affected by many sources including environmental
conditions such as sun exposure, building heating and air conditioning, and
air pollution
can accelerate deterioration of the condition and appearance of skin.
Additionally, certain
diseases can affect the appearance of the skin. Deterioration of the
appearance of the skin
may include, but is not limited to, wrinkles, loss of firmness and elasticity
of the skin, age
spots, discolorations, and dryness. In addition, individual factors such as
diet, stress, age
and genetics may affect the appearance of the skin.
Various compositions and methods for manipulating the appearance of the skin
have been reported. For example, international patent application, published
under
number WO/2004/100889, describes anti-ageing agents, including 3,3' -
thiodipropionic
acid or derivatives thereof for improving the aesthetic appearance of skin.
Another
method of manipulating the quality of the skin is cosmetic surgery. It has
been reported
CA 02701378 2010-04-22
-2-
that seaweed extracts can be incorporated in compositions for use on the skin
(see, for e.g.,
United States patent application 2004/0219124).
SUMMARY OF THE INVENTION
In accordance with one aspect of the invention there is provided a cosmetic
composition for applying to skin. The composition includes a liquid, and
dispersed in the
liquid an amount of fucoidan, an amount of beta glucan, and an amount of a
marine
extract, wherein the cosmetic composition improves the appearance of the skin
to which
the composition is applied.
The composition may further include water as the liquid. The composition may
further include the liquid being a glycolic acid-salicylic acid solution. The
composition
may further include the liquid being water and a glycolic acid-salicylic acid
solution. The
composition may further include the marine extract being any one or more of a
green
seaweed, a brown seaweed, an exopolysaccharide, or an algae. The composition
may
further include the marine extract being Ulva lactuta, Alteromonas maclodeii,
Astaxanthin, or EckIonia cava.
In another aspect of the invention, the composition may include an amount of
tourmaline. In another aspect of the invention, the composition may include an
amount of
volcanic obsidian. In another aspect of the invention, the composition may
include an
amount of Aloe barbadensis. In another aspect of the invention, the
composition may
include an amount of hydrolyzed pearl nacre. In another aspect of the
invention, the
composition may include an amount of chitosan. In another aspect of the
invention, the
composition may include an amount of a phospholipid.
In accordance with another aspect of the invention, the composition may
include
an amount of glyceryl stearate. In another aspect of the invention, the
composition may
include an amount of stearic acid. In another aspect of the invention, the
composition may
include an amount of cetearyl alcohol. In another aspect of the invention, the
composition
may include an amount of ceteareth 20. In another aspect of the invention, the
composition may include an amount of isopropyl palmitate. In another aspect of
the
invention, the composition may include an amount of ascorbyl polypeptide. In
another
aspect of the invention, the composition may include an amount of tocotrienol.
In another
aspect of the invention, the composition may include an amount of tocopheryl
acetate. In
CA 02701378 2010-04-22
-3-
another aspect of the invention, the composition may include an amount of
ergocalciferol.
In another aspect of the invention, the composition may include an amount of
niacinamide.
In another aspect of the invention, the composition may include an amount of
ferulic acid.
In another aspect of the invention, the composition may include an amount of
Camellia
sinensis. In another aspect of the invention, the composition may include an
amount of
Centella asiatica. In another aspect of the invention, the composition may
include an
amount of Chamomile matricaria. In another aspect of the invention, the
composition
may include an amount of Echinacea augustifolia.
In accordance with another aspect of the invention, the composition may
include
an amount of Ginko biloba. In another aspect of the invention, the composition
may
include an amount of butylene glycol. In another aspect of the invention, the
composition
may include an amount of phenoxyethanol. In another aspect of the invention,
the
composition may include an amount of caprylyl glycol. In another aspect of the
invention,
the composition may include an amount of sorbic acid. In another aspect of the
invention,
the composition may include an amount of peg8/SMDI copolymer. In another
aspect of
the invention, the composition may include an amount of superoxide dismutase
liposomes.
In another aspect of the invention, the composition may include an amount of N-
acetyl
carnitine. In another aspect of the invention, the composition may include an
amount of
alpha lipoic acid. In another aspect of the invention, the composition may
include an
amount ofl-arginine.
In accordance with another aspect of the invention, the composition may
include
an amount of Glycine sofa. In another aspect of the invention, the composition
may
include an amount of Rumex crispis. In another aspect of the invention, the
composition
may include an amount of Vitis vinifera. In another aspect of the invention,
the
composition may include an amount of hinokitiol. In another aspect of the
invention, the
composition may include an amount of retinol. In another aspect of the
invention, the
composition may include an amount of Panax ginseng. In another aspect of the
invention,
the composition may include an amount of allantoin. In another aspect of the
invention,
the composition may include an amount of kaolin. In another aspect of the
invention, the
composition may include an amount of bentonite. hi another aspect of the
invention, the
composition may include an amount of Undaria pinnatifida. In another aspect of
the
invention, the composition may include an amount of diatomite silica. In
another aspect
CA 02701378 2010-04-22
-4-
of the invention, the composition may include an amount of n-acetyl
glucosamine. In
another aspect of the invention, the composition may include an amount of
phytoplankton.
In another aspect of the invention, the composition may include an amount of a
member of
the Skeletonema. In another aspect of the invention, the composition may
include an
amount of a member of the Thalassiosira. In another aspect of the invention,
the
composition may include an amount of a member of the Chaetoceros. In another
aspect of
the invention, the composition may include an amount of Fucus vesiculosus. In
another
aspect of the invention, the composition may include an amount of sodium pCA.
In
another aspect of the invention, the composition may include an amount of
magnesium
ascorbyl phosphate. In another aspect of the invention, the composition may
include an
amount of epigallocatechin (EGCG). In another aspect of the invention, the
composition
may include an amount of ellagic acid. In another aspect of the invention, the
composition
may include an amount of sodium citrate. In another aspect of the invention,
the
composition may include an amount of tetrasodium EDTA. In another aspect of
the
invention, the composition may include an amount of an emollient. In another
aspect of
the invention, the composition may include an amount of a sunscreen. The
sunscreen may
be an organic sunscreen. The sunscreen may be an inorganic sunscreen. In
another aspect
of the invention, the composition may include an amount of a silicone
surfactant. The
silicone surfactant may be cyclomethicone or dimethicone copolyol.
In another aspect of the invention, the composition may include an amount of a
preservative. The preservative may be methylisothiazolinone, cetylsyredinium
chloride,
silver, benzyl pCA, or polyaminopropyl biguamide. In another aspect of the
invention, the
composition may include an amount of an aqueous botanical antioxidant. The
aqueous
botanical antioxidant may be hydroxytyrosol, rutin, silymarin, turmeric,
genistein, apple,
green coffee, quercetin, or rosemary. In another aspect of the invention, the
composition
may include an amount of a humectant. The humectant may be a glycerol, a
sorbitol, or a
polyol. In another aspect of the invention, the composition may include an
amount of a
thickener. The thickener may be a cosmetic gum. The cosmetic gum may be
alginic acid,
xanthan gum, cellulose gum, hydroxtethyl cellulose, dextrin, agar, guar gum,
phycocolloid, ghatti gum, cellulose ester, modified potato starch, or pectin.
In another
aspect of the invention, the composition may include an amount of an oligomer.
The
oligomer may be iso-olefin, isodecane, hydrogenated polybutene, hydrogenated
CA 02701378 2010-04-22
-5-
polydecene, polycaprolactone, fibronectin or polyethylene glycol. In another
aspect of the
invention, the composition may include an amount or combination of an amino
acid. The
amino acid may be present in its natural form. The amino acid may be a
synethetic amino
acid.
In another aspect of the invention, the composition may include an amount of a
mineral and its salt. The mineral may be calcium, magnesium, manganese, or
zinc. In
another aspect of the invention, the composition may include an amount of a
base. The
base may be a soluble plant butter, a soluble plant wax, an anhydrous plant
based
cholesterol base, a phycocolloid gel base, a prepared polyethylene glycol
ointment base, or
a prepared carbomer gel base. In another aspect of the invention, the
composition may
include an amount of a marine component. The marine component may be an
Enteromorpha species, a Porphyra species, a Chrondus species, a Laminares
species, a
kelp species or a FucaIs species. In another aspect of the invention, the
composition may
include an amount of an anti-inflammatory agent. The anti-inflammatory agent
may be
Glycyrrhiza glabria, Boswellia serrate, Curcuma longa, turmeric, Arnica
montana,
silymarin, water melon, calendula, eidelweiss, or ginger. In another aspect of
the
invention, the composition may include an amount of a surfactant. The
surfactant may be
amphoteric. The surfactant may be cocomidopropyl betaine. The surfactant may
be non-
ionic. The surfactant may be cocoglucoside or coco polyglucose. The surfactant
may be
cationic. The surfactant may be lauryl dimoniumhydrolysed collagen.
In another aspect of the invention, the composition may include an amount of
a percutaneous penetration enhancer. The percutaneous penetration enhancer may
be
polyethylene glycol or oleic acid. In another aspect of the invention, the
composition may
include an amount of a preservative enhancer. The preservative enhancer may be
ethylhexyl glycerin, benzethonym chloride, or a hydantoin/PCB blend. In
another aspect
of the invention, the composition may include an amount of a vasodilator. The
vasodilator
may be adenosine triphosphate or liposomal 1 arginine. In another aspect of
the invention,
the composition may include an amount of a vasoconstrictor. The
vasoconstrictor may be
sea buckthorn, milk thistle, or marshmallow root. In another aspect of the
invention, the
composition may include an amount of a whitening agent. In another aspect of
the
invention, the composition may include an amount of a tyrosinase inhibitor.
The
tyrosinase inhibitor may be selected from the Glycyrrhiza species. The
tyrosinase
- 6 -
inhibitor may be a favnoid, a polyphenol, an isoflavon, or a coumarin. In
another aspect of the
invention, the composition may include an amount of octadecnedioic acid. In
another aspect of the
invention, the composition may include an amount of chalcones.
In another aspect of the invention the composition may be absorbed by human
skin. In
another aspect of the invention the composition may be homogeneous.
Various embodiments of the invention relate to a cosmetic composition for
applying to
skin, the composition comprising: from about 1.5 wt.% to about 5.5 wt.% of a
seaweed complex
consisting essentially of Ulva lactuca, Alteromonas macleodii and Astaxanthin
in a ratio of about
7:1.5:1.5 by weight; 0.15 wt.% tourmaline; 0.05 wt.% volcanic obsidian; from
about 8 wt.% to
about 12 wt.% of a 1:1 B-glucan:fucoidan ratio by weight mixture, said B-
glucan consisting of a
1:1 ratio by weight of yeast B- glucan and fungi B - glucan; 10 wt.% of
aqueous Aloe barbadensis
leaf extract; 1.5 wt.% hydrolyzed pearl nacre; 1.0 wt.% chitosan; 10 wt.%
phospholipids; 3 wt.%
glyceryl stearate; 3 wt.% stearic acid; 3 wt.% cetearyl alcohol; 3 wt.%
ceteareth 20; 3 wt.%
isopropyl palmitate; 6 wt.% of a 3:1:1:1:1 ratio by weight mixture of ascorbyl
polypeptide,
tocotrienol, tocopheryl acetate, ergocalciferol, and niacinamide; 1.5 wt.% of
a 1:1:1:1:1 ratio by
weight mixture of an aqueous extract of Camellia sinensis, Centella asiatica,
Matricaria
chamomilla, Echinacea angustifolia, and Ginkgo biloba; 2.5 wt.% of a
1.5:0.5:0.25:0.25:0.25 ratio
by weight mixture of butylene glycol, phenoxyethanol, caprylyl glycol, sorbic
acid, and
PEG8/SMDI copolymer; 8 wt.% of aqueous superoxide dismutase liposomes; 20.8
wt.% purified
water; and 10 wt.% of an aqueous glycolic acid-salicylic acid solution
neutralized with ammonium
hydroxide, ammonium glycolate or sodium lactate.
Various embodiments of the invention relate to a cosmetic composition for
applying to
skin, the composition comprising: from about 3.5 wt.% to about 5.5 wt.% of a
seaweed complex
consisting essentially of Ecklonia cava, Alteromonas macleodii and Astaxanthin
in a ratio of about
7:1.5:1.5 by weight; 0.15 wt.% tourmaline; 0.05 wt.% volcanic obsidian; from
about 8 wt.% to
about 10 wt.% of a 1:1 B- glucan:fucoidan ratio by weight mixture, said B -
glucan consisting of
a 1:1 ratio by weight of yeast B - glucan and fungi B- glucan; 10 wt.% of
aqueous Aloe barbadensis
leaf extract; 1.5 wt.% hydrolyzed pearl nacre; 1.0 wt.% chitosan; 10 wt.%
phospholipids; 4 wt.%
glyceryl stearate; 4 wt.% stearic acid; 4 wt.% cetearyl alcohol; 4 wt.%
ceteareth 20; 4 wt.%
Date Recue/Date Received 2021-09-02
- 6a -
isopropyl palmitate; 6 wt.% of a 3:1.5:1.5:0.5 ratio by weight mixture of
tocotrienol, tocopheryl
acetate, niacinamide, and retinal; 2.5 wt.% of a 1:1:1:1:1:1 ratio by weight
mixture of the aqueous
extracts of Vitis vinifera, Matricaria chamomilla, Echinacea angustifolia,
Panax ginseng, Glycine
soja, and allantoin; 2.5 wt.% of a 1.5:0.5:0.25:0.25:0.25 ratio by weight
mixture of butylene glycol,
phenoxyethanol, caprylyl glycol, sorbic acid, and PEG8/SMDI copolymer; 8 wt. %
of aqueous
superoxide dismutase liposomes; 14.8 wt.% purified water; and 10 wt.% of an
aqueous glycolic
acid-salicylic acid solution neutralized with ammonium hydroxide, ammonium
glycolate or
sodium lactate.
Various embodiments of the invention relate to a cosmetic composition for
applying to
skin, the composition comprising: 5.5 wt.% of a seaweed complex consisting
essentially of
Undaria pinnatifida and Astaxanthin in a ratio of about 7:5 by weight; 0.15
wt.% tourmaline; 0.05
wt.% volcanic obsidian; 8 wt.% of a 1:1 B - glucan:fucoidan ratio by weight
mixture, said B -
glucan consisting of a 1:1 ratio by weight of yeast B - glucan and fungi B -
glucan; 10 wt.% of
aqueous Aloe barbadensis leaf extract; 1.5 wt.% hydrolyzed pearl nacre; 1.0
wt.% chitosan; 10
wt.% phospholipids; 4 wt.% glyceryl stearate; 4 wt.% stearic acid; 4 wt.%
cetearyl alcohol; 4 wt.%
ceteareth 20; 4 wt.% isopropyl palmitate; 6 wt.% of a 3:1.5:1.5:0.5 ratio by
weight mixture of
tocotrienol, tocopheryl acetate, niacinamide, and crithmum maritimum; 2.5 wt.%
of a 1:1:1:1:1:1
ratio by weight mixture of an aqueous extract of Vitis vinifera, Matricaria
chamomilla, Echinacea
angustifolia, Panax ginseng, Glycine soja, and allantoin; 2.5 wt.% of a
1.5:0.5:0.25:0.25:0.25 ratio
by weight mixture of butylene glycol, phenoxyethanol, caprylyl glycol, sorbic
acid, and
PEG8/SMDI copolymer; 8 wt.% of aqueous superoxide dismutase liposomes; 14.8
wt.% purified
water; and 10 wt.% of an aqueous glycolic acid-salicylic acid solution
neutralized with ammonium
hydroxide, ammonium glycolate or sodium lactate.
Various embodiments of the invention relate to a cosmetic composition for
applying to
skin, the composition comprising: from 4 wt.% to 10 wt.% of a seaweed complex
consisting
essentially of Ulva lactuca, Alteromonas macleodii and Astaxanthin in a ratio
of about 7:1.5:1.5
by weight; 0.15 wt.% tourmaline; 0.05 wt.% volcanic obsidian; from 10 wt.% to
16 wt.% of a 1:1
B- glucan:fucoidan ratio by weight mixture, said B - glucan consisting of a
1:1 ratio by weight of
yeast B - glucan and fungi B - glucan; 12 wt.% of aqueous Aloe barbadensis
leaf extract; 0.9 wt.%
hydrolyzed pearl nacre; 0.6 wt.% chitosan; 3.5 wt.% of a 1.5:1.5:0.5 ratio by
weight mixture of N-
acetyl camitine, a-lipoic acid, and L-arginine; 6.4 wt.% phospholipids; 1.6
wt.% sodium
Date Recue/Date Received 2021-09-02
- 6b -
hyaluronate; 3 wt.% glyceryl stearate; 3 wt.% stearic acid; 3 wt.% cetearyl
alcohol; 3 wt.%
ceteareth 20; 3 wt.% isopropyl palmitate; 5 wt.% of a 3:1:0.5:0.5:0.5:0.5
ratio by weight mixture
of ascorbyl polypeptide, tocotrienol, tocopheryl acetate, ergocalciferol,
ferulic acid, and
niacinamide; 2 wt.% of a 1:1:1:1 ratio by weight mixture of Glycine soja,
Rumex crispis, Vitis
vinifera, and hinokitiol; 2.5 wt.% of a 1:1:1:1:1 ratio by weight mixture of
the aqueous extracts of
Camellia sinensis, Centella asiatica, Matricaria chamomilla, Echinacea
angustifolia, and Ginkgo
biloba; 3.5 wt.% of a 1.5:0.5:0.25:0.25:0.25 ratio by weight mixture of
butylene glycol,
phenoxyethanol, caprylyl glycol, sorbic acid, and PEG8/SMDI copolymer; 8 wt.%
of aqueous
superoxide dismutase liposomes; and 18.8 wt.% purified water.
Other aspects and features of the present invention will become apparent to
those ordinarily
skilled in the art upon review of the following description of specific
embodiments of the
invention.
DETAILED DESCRIPTION
Any terms not directly defined herein shall be understood to have the meanings
commonly
associated with them as understood within the art of the invention. Certain
terms are discussed
below, or elsewhere in the specification, to provide additional guidance to
the practitioner in
describing the compositions, devices, methods and the like of embodiments of
the invention, and
how to make or use them. It will be appreciated that the same thing may be
said in more than one
way. Consequently, alternative language and synonyms may be used for any one
or more of the
terms discussed herein. No significance is to be placed upon whether or not a
term is elaborated
or discussed herein. Some synonyms or substitutable methods, materials and the
like are provided.
Recital of one or a few synonyms or equivalents does not exclude use of other
synonyms or
equivalents, unless it is explicitly stated. Use of examples in the
specification, including examples
of terms, is for illustrative purposes only and does not limit the scope and
meaning of the
embodiments of the invention herein.
In accordance with one aspect of the invention there is provided a cosmetic
composition
for applying to skin. The composition is comprised of a liquid and dispersed
in the liquid is an
amount or form of fucoidan, an amount or form of beta glucan, and an amount of
a marine extract.
When applied to the skin, the cosmetic composition improves the appearance of
the skin. Improved
appearance of the skin may be associated with any one of the following but is
not limited to the
Date Recue/Date Received 2021-09-02
- 6c -
following: decreased skin wrinkles, increased skin smoothness, decreased
roughness of the skin,
increased luminosity of the skin, increased clarity of the skin, increased
firmness of the skin,
increased tautness of the skin, decreased irritation of the skin, decreased
skin-associated
inflammation, improved skin
Date Recue/Date Received 2021-09-02
CA 02701378 2010-04-22
-7-
tone, improved hydration of the skin, decreased dryness of the skin,
improvements in skin
discoloration, and decreased breakouts of skin conditions such as acne.
Optionally, the liquid may be water, and can include but is not limited to
distilled
water. Optionally, the liquid may be a glycolic acid-salicylic acid solution.
Further, and
optionally, the liquid may be a combination of water, including but not
limited to distilled
water, and a glycolic acid-salicylic acid solution. Optionally, the marine
extract used in
the combination may include one or more of a green seaweed, a brown seaweed,
an
exopolysaccharide, or an algae. A green seaweed may include but is not limited
to the
Ulva genus and particularly Ulva lactuta. A brown seaweed may include but is
not
limited to laminariales, which is commonly known as kelp, and fucals. A brown
seaweed
may include Ecklonia cava. An expolysaccharide is understood to be a high-
molecular-
weight polymer of sugar residues. Algae may include but are not limited to
members of
the following groups: Archaeplastida, Rhizaria, Excavata, Excavata, Chromista,
and
Alveolata. Optionally, the composition may include a marine extract selected
from any
one or more of the following: Ulva lactuta, Alteromonas maclodeii,
Astaxanthin, or
Ecklonia cava.
Optionally, the composition may include an amount of tourmaline. Optionally,
the
composition may include an amount of volcanic obsidian. The composition may
include
an amount of Aloe barbadensis. The composition may further include an amount
of
hydrolyzed pearl nacre. Optionally, the composition may include an amount of
chitosan.
Optionally, the composition may include an amount of a phospholipid. A
phospholipid is
understood as being any of a variety of phosphorous-containing lipids that are
composed
mainly of fatty acids, a phosphate group, and a simple organic molecule.
Optionally, the
composition may include an amount of glyceryl stearate. Optionally, the
composition may
include an amount of stearic acid. Optionally, the composition may include an
amount of
cetearyl alcohol.
Further, the composition may include an amount of ceteareth 20. The
composition
may also include an amount of isopropyl palmitate. Optionally, the composition
may
include an amount of one or more of the following: ascorbyl polypeptide,
tocotrienol,
tocopheryl acetate, ergocalciferol, niacinamide, or ferulic acid.
Optionally, the
composition may include an amount of Camellia sinensis. Further, the
composition may
include an amount of one or more of the following: Centella asiatica,
Chamomile
CA 02701378 2010-04-22
-8-
matricaria, Echinacea augustifolia, or Ginko biloba. Optionally, the
composition may
include an amount of one or more of the following: butylene glycol,
phenoxyethanol,
caprylyl glycol, sorbic acid, peg8/SMDI copolymer, superoxide dismutase
liposomes, N-
acetyl carnitine, alpha lipoic acid, or 1-arginine.
Further, the composition may include an amount of one or more of the
following:
Glycine sofa, Rumex crispis, Vitis vinffera, hinokitiol, retinol, Panax
ginseng, allantoin,
kaolin, bentonite, Undaria pinnatifida, diatomite silica, or n-acetyl
glucosamine. The
composition may further include an amount of a phytoplankton species. The
composition
may include an amount of a member of the Skeletonema. The composition may
include an
amount of a member of the Thalassiosira. The composition may include an amount
of a
member of the Chaetoceros.
Further, the composition may include an amount of Fucus vesiculosus. The
composition may include an amount of one or more of the following: sodium pCA,
magnesium ascorbyl phosphate, epigallocatechin (EGCG), ellagic acid, sodium
citrate, or
tetrasodium EDTA. The composition may include an amount of an emollient. An
emollient is understood as being a material used for the prevention or relief
of dryness, as
well as for the protection of the skin.
The composition may include an amount of a sunscreen. The sunscreen may be an
organic sunscreen. According to an embodiment of the invention, the
composition may
include from approximately 0.1 to approximately 10%, and preferably from
approximately
1 to approximately 5% by weight of an organic sunscreen material. The
sunscreen may be
an inorganic sunscreen. According to an embodiment of the invention, the
composition
may include an inorganic sunscreen such as titanium dioxide or zinc oxide,
having an
average particle size of from 1 to 300 nm, or iron oxide, having an average
particle size of
from 1 to 300 nm, or silica, having an average particle size of from 1 to 100
nm.
The composition may include an amount of a silicone surfactant, which may
include but is not limited to cyclomethicone or dimethicone copolyol. The
composition
may include an amount of a preservative. Examples of a preservative include
but are not
limited to methylisothiazolinone, cetylsyredinium chloride, silver, benzyl
pCA, or
polyaminopropyl biguamide.
Further, the composition may include an amount of an aqueous botanical
antioxidant. Examples of an aqueous botanical antioxidant include, but are not
limited to:
CA 02701378 2010-04-22
-9-
hydroxytyrosol, rutin, silymarin, turmeric, genistein, apple, green coffee,
quercetin, or
rosemary. Further, the composition may include an amount of a humectant. The
humectant may be a glycerol, a sorbitol, or a polyol. The humectant chosen is
not limited
to the foregoing list of humectants.
The composition may include an amount of a thickener, including but not
limited
to, a cosmetic gum. The cosmetic gum may include, but is not limited to,
alginic acid,
xanthan gum, cellulose gum, hydroxtethyl cellulose, dextrin, agar, guar gum,
phycocolloid, ghatti gum, cellulose ester, modified potato starch, or pectin.
Further, the
composition may include an amount of an oligomer, including but not limited
to, an
oligomer of iso-olefin, isodecane, hydrogenated polybutene, hydrogenated
polydecene,
polycaprolactone, fibronectin or polyethylene glycol. An oligomer is
understood as
representing a few of the aforementioned monomeric units.
The composition may include an amount of an amino acid. The composition may
include a combination of amino acids, for example, a peptide comprised of
amino acids.
The amino acid may be in its natural form or it may be a synthetic amino acid.
An amino
acid may be described as, for example, polar, non-polar, acidic, basic,
aromatic or neutral.
A polar amino acid is an amino acid that may interact with water by hydrogen
bonding at
biological or near-neutral pH. The polarity of an amino acid is an indicator
of the degree
of hydrogen bonding at biological or near-neutral pH. Examples of polar amino
acids
include serine, praline, threonine, cysteine, asparagine, glutamine, lysine,
histidine,
arginine, aspartate, tyrosine and glutamate. Examples of non-polar amino acids
include
glycine, alanine, valine leucine, isoleucine, methionine, phenylalanine, and
tryptophan.
Acidic amino acids have a net negative charge at a neutral pH. Examples of
acidic amino
acids include aspartate and glutamate. Basic amino acids have a net positive
charge at a
neutral pH. Examples of basic amino acids include arginine, lysine and
histidine.
Aromatic amino acids are generally nonpolar, and may participate in
hydrophobic
interactions. Examples of aromatic amino acids include phenylalanine, tyrosine
and
tryptophan. Tyrosine may also participate in hydrogen bonding through the
hydroxyl
group on the aromatic side chain. Neutral, aliphatic amino acids are generally
nonpolar
and hydrophobic. Examples of neutral amino acids include alanine, valine,
leucine,
isoleucine and methionine. An amino acid may be described by more than one
descriptive
CA 02701378 2010-04-22
- 1 0 -
category. Amino acids sharing a common descriptive category may be
substitutable for
each other in a peptide.
An amino acid residue may be generally represented by a one-letter or three-
letter
designation, corresponding to the trivial name of the amino acid, in
accordance with the
following Table A.
Amino acids comprising the peptides described herein will be understood to be
in
the L- or D- configuration.
Amino acids described herein, may be modified by
methylation, amidation, acetylation or substitution with other chemical groups
which may
change the circulating half-life of the peptide without adversely affecting
their biological
activity. Additionally, a disulfide linkage may be present or absent in the
peptides of the
invention.
Nonstandard amino acids may occur in nature, and may or may not be genetically
encoded.
Examples of genetically encoded nonstandard amino acids include
selenocysteine, sometimes incorporated into some proteins at a UGA codon,
which may
normally be a stop codon, or pyrrolysine, sometimes incorporated into some
proteins at a
UAG codon, which may normally be a stop codon. Some nonstandard amino acids
that
are not genetically encoded may result from modification of standard amino
acids already
incorporated in a peptide, or may be metabolic intermediates or precursors,
for example.
Examples of nonstandard amino acids include 4-hydroxyproline, 5-hydroxylysine,
6-N-
methyllysine, gamma-carboxyglutamate, desmosine, selenocysteine, ornithine,
citrulline,
lanthionine, 1-aminocyclopropane-1 -carboxylic acid, gamma-aminobutyric acid,
camitine,
sarcosine, or N-formylmethionine. Synthetic variants of standard and non-
standard amino
acids are also known and may include chemically derivatized amino acids, amino
acids
labeled for identification or tracking, or amino acids with a variety of side
groups on the
alpha carbon. Examples of such side groups are known in the art and may
include
aliphatic, single aromatic, polycyclic aromatic, heterocyclic, heteronuclear,
amino,
alkylarnino, carboxyl, carboxamide, carboxyl ester, guanidine, amidine,
hydroxyl, alkoxy,
mercapto-, alkyhnercapto-, or other heteroatom-containing side chains. Other
synthetic
amino acids may include alpha-amino acids,
non-alpha
CA 02701378 2010-04-22
-11-
Table A. Nomenclature and abbreviations of the 20 standard L-amino acids
commonly found in naturally occurring peptides
Full name Three-letter abbreviation One-letter abbreviation
Alanine Ala A
Cysteine Cys
Aspartic add Asp
Glutamic acid Glu
Phenylalanine Phe
Glycine Gly
Histidine His
Isoleucine Ile
Lysine Lys
Leucine Leu
Methionine Met
Asparagine Asp
Proline Pro
Glutamine Gin
Arginine Arg
Serine Ser
Threonine Thr
Valine Val V
Tryptophan Trp
Tyrosine Tyr
-12-
amino acids such as beta-amino acids, des-carboxy or des-amino acids.
Synthetic variants
of amino acids may be synthesized using general methods known in the art, or
may be
purchased from commercial suppliers, for example RSP Amino Acids LLC (Shirley,
MA).
Additionally, the composition may include an amount of a mineral and its salt.
The mineral may be calcium, magnesium, manganese, or zinc. The mineral chosen
is not
limited to the aforementioned minerals. The composition may include an amount
of a
base, including but not limited to, a soluble plant butter, a soluble plant
wax, an anhydrous
plant based cholesterol base, a phycocolloid gel base, a prepared polyethylene
glycol
ointment base, or a prepared carbomer gel base. The composition may include an
amount
of a marine component including, but not limited to, any one or more of the
following: an
Enteromorpha species, a Porphyra species, a Chrondus species, a Laminares
species, a
kelp species or a Fucals species. The marine component or seaweed utilized in
the
composition can be selected from the group consisting of brown algae, red
algae and green
algae. Further the marine component or seaweed utilized in the composition can
be
selected by pre-determining the content of any one of the following in the
marine
component: natural amino acids, fatty acids and their glycosyl derivatives,
sterols, natural
antimicrobials, ursolic acid, or mono / polysaccharides. Methods for analyzing
the above
are known in the art and include but are not limited to the techniques
disclosed in
Sanchez-Machado et at. (2004) Biomedical Chromatography 18(3):183-90.
Further, the composition may include an amount of an anti-inflammatory agent.
The anti-inflammatory agent may include, but is not limited to, any one or
more of the
following: Glycyrrhiza glabria, Boswellia serrate, Curcuma longa, turmeric,
Arnica
montana, silymarin, water melon, calendula, eidelweiss, or ginger.
The composition may include an amount of a surfactant. The surfactant may be
amphoteric. They surfactant may be, but is not limited to, cocomidopropyl
betaine. The
surfactant may be non-ionic. The surfactant may be, but is not limited to,
cocoglucoside
or coco polyglucose. The surfactant may be cationic. The surfactant may be,
but is not
limited to, lauryl dimoniumhydrolysed collagen.
The composition may include an amount of a percutaneous penetration enhancer
including, but not limited to, any one or more of the following: polyethylene
glycol or
oleic acid. The composition may include an amount of a preservative enhancer
including,
but not limited to, any one or more of the following: ethylhexyl glycerin,
benzethonym
CA 2701378 2018-08-09
CA 02701378 2010-04-22
-13-
chloride, or a hydantoin/PCB blend. Further, the composition may include an
amount of a
vasodilator including, but not limited to, any one of the following: adenosine
triphosphate
or liposomal I arginine.
Further, the composition may include an amount of a vasoconstrictor including,
but
not limited to, any one or more of the following: sea buckthorn, milk thistle,
or
marshmallow root. Additionally, the composition may include an amount of a
whitening
agent. The composition may include an amount of a tyrosinase inhibitor
including, but not
limited to, an inhibitor selected from the Glycyrrhiza species. The tyrosinase
inhibitor
may include, but is not limited to, a favnoid, a polyphenol, an isoflavon, or
a coumarin.
Further, the composition may include an amount of octadecnedioic acid or
chalcones or a
combination thereof.
Although the foregoing invention has been described in some detail by way of
illustration and example for purposes of clarity of understanding, it will be
readily
apparent to those of skill in the art in light of teachings of this invention
that changes and
modification may be made thereto without departing from the spirit or scope of
the
invention.
EXAMPLES
Example 1. Composition with acidified fucoidan, beta glucan, and ascorbyl
polypeptide
In accordance with an embodiment of the present invention, a composition is
described having the components shown in Table 1 below. The methodology
employed in
producing the composition using the amounts described in Table 1 below is
generally as
follows.
Method Sequence No. 1
In a first mixture, neutralized glycolic and salicyclic acid solution (source:
Spectrum Chemical; Gardena, California) was heated in a stainless steel
container to
approximately 85 degrees Celsius. The seaweed complex consisting of Ulva
lactuta,
Alteromonas maclodeii, and astaxanthin (source: Unipex Innovations,
Mississauga,
Ontario) was added to the heated glycolic acid mixture. The combined mixture
was mixed
for approximately 45 to 60 minutes.
CA 02701378 2010-04-22
-14-
Table 1. Components of an embodiment of the Invention
Sequence No. Component Amount (70
by weight)
Glycolic acid-salicylic acid solution (ratio: 8:1) 10
(neutralized with ammonium hydroxide; ammonium
glycolate, sodium lactate can also be added to
obtain a preferable pH of -4)
Seaweed complex containing Ulva lactuta, 5.5
Alteromonas maclodeii and Astaxanthin (ratio:
7:1.5:1.5)
Distilled Water 20.8
Tourmaline (0.15%) and volcanic obsidian (0.05%) 0.2
Beta glucan (yeast and specified medicinal fungi 8
extract combined in a 1:1 ratio) and fucoidan
complex (beta glucan and fucoidan are present in a
1:1 ratio)
Aqueous Aloe barbadensis extract 10
Hydrolyzed pearl nacre (1.5%) and chitosan (0.5%) 2.5
Phospholipids 10
Glyceryl stearate, stearic acid, cetearyl alcohol, 15
ceteareth 20, isopropyl palmitate complex (each of
these products is added at an equal amount)
Ascorbyl polypeptide, toc,otrienol, tocopheryl 6
acetate, ergocalciferol, niacinamide complex (ratio of
these products: 3:1:1:1:1:1)
Aqueous extract of Camellia sinensis, ConteIla 1.5
asiatica, Chamomile matricaria, Echinacea
augustifolia, Ginko biloba (each of these products is
added at an equal amount)
Complex of butylene glycol, phenoxyethanol, 2.5
caprylyl glycol, sorbic acid, peg8/SMDI copolymer
(these products are added at the following ratio: 1.5:
0.5: 0.25 : 0.25 : 0.25)
lii Aqueous superoxide dismutase liposomes 8
CA 02701378 2010-04-22
-15-
A polarized water was developed by adding tourmaline and obsidian (source:
Soliance; Paris, France) to the distilled water. The beta glucan (source:
Biothera; Eagen,
Minnesota) and fucoidan (source: Marinova; Cambridge, Tasmania, Australia)
complex
was dissolved in one-third of the polarized water. The dissolved beta glucan-
fucoidan
complex was added to the glycolic acid mixture (previously described above)
and the
entire solution was maintained at approximately 85 degrees Celsius for
approximately 30
minutes. While maintaining mixing of the mixture, aqueous Aloe barbedensis
leaf extract
(source: Tri K Industries; Northvale, New Jersey) was added and the entire
mixture was
allowed to cool for approximately 30 minutes. Thereafter, hydrolyzed pearl
nacre (source:
Active Concepts; Lincolnton, North Carolina) and chitosan (source: Alfa Chem;
Kings
Point, New York) were added to the cooled mixture. The entire mixture was
allowed to
cool to approximately 30 degrees Celsius. Thereafter, phospholipids (source:
Arch
Personal Care; Norwalk, Connecticut) were added to the cooled mixture.
Method Sequence No. 2
In a further mixture, glyceryl stearate, stearic acid, cetearyl alcohol,
ceteareth 20,
and isopropyl palmitate complex (source: Hallstar Corp.; Chicago, Illinois)
were heated to
75 degrees Celsius in a 200 kg stainless steel vat. The mixture was mixed by
continuous
stirring for approximately 60 minutes.
Separately, the remaining two-thirds of polarized water (as described above)
was
used to dissolve the ascorbyl polypeptide-tocotrienol complex (source: Arch
Personal
Care). The ascorbyl polypeptide-tocotrienol complex was allowed to dissolve in
the
polarized water for approximately one hour. Thereafter, aqueous extracts of
Camellia
sinensis, Centella asiatica, Chamomile matricaria, Echinacea augustifolia, and
Ginko
biloba (source: Active Organics; Louisville, Texas) were added to the ascorbyl
polypeptide ¨ tocotrienol complex.
Method Sequence No. 3
In a further mixture, butylene glycol, phenoxyethanol, caprylyl glycol, sorbic
acid,
and peg8/SMDI copolymer were added at the ratio described above to make a
complex
(source: International Specialty Products; Mississauga, Ontario). Thereafter,
this complex
was added to the glyceryl stearate complex mixture described above, the
glyceryl stearate
CA 02701378 2010-04-22
-16-
complex having been maintained at approximately 75 degrees Celsius.
Thereafter, the
ascorbyl polypeptide-tocotrienol complex, which had previously been mixed with
the
aqueous extracts described above, was added and agitation of the mixture
continued at
approximately 75 degrees Celsius. Thereafter, the entire mixture was cooled
down to
approximately 30 degrees Celsius.
Thereafter, the mixture developed from Method Sequence No. 1 was added to the
mixture. The mixture was allowed to cool down to approximately 20 degrees
Celsius and
liposomal superoxide dismutase (source: Arch Personal Care) was added. The
mixture
developed in Example 1 is a homogenous mixture.
In a modification to the above, the seaweed complex can be present as 1.5% of
the
formulation instead of 5.5% as described in Table 1. Further, the beta glucan
and fucoidan
complex can be present as 12% of the formulation instead of 8% as described in
Table 1.
Testing efficacy of Example 1
To test the efficacy of the mixture described in Example 1, the mixture was
used
by human subjects to determine the effect on the appearance of their skin. The
results
routinely showed that following application of the mixture described in
Example 1, the
human subjects exhibited improved appearance of their skin.
Serving as an example, JT, a female under the age of 30 years of age, used the
formulation defined in Example 1. Prior to using the formulation of Example 1,
JT had
exhibited sensitive skin and a tendency to have acne breakouts. JT also
described
numerous fine lines being visible in her forehead region. On day 0, JT began
using an
amount approximately equivalent to the size of a quarter and used
approximately that
amount each morning on her skin. After one week, JT described her skin as
being brighter
and with an improved complexion. After two weeks, JT described that the fine
lines on
her skin were blurred. After one month, JT described that her skin had less
breakouts and
was very smooth. JT identified at least the following attributes as being
associated with
the formulation defined in Example 1: increased smoothness and brightness of
the skin,
decreased lines on JT's forehead, and decreased breakouts of acne. Further, JT
identified
the formulation of Example 1 as being associated with decreased dryness of the
skin.
It was determined that the formulation/mixture of Example 1 was absorbed by
human skin.
CA 02701378 2010-04-22
-17-
Example 2. Composition with non-acidified fucoidan and beta glucan
In accordance with a further embodiment of the present invention, a
composition is
described having the components shown in Table 2. The methodology employed in
producing the composition using the amounts described in Table 2 is generally
as follows.
Method Sequence No. 1
In a first mixture, a polarized water was developed by adding tourmaline and
obsidian (source: Soliance) to the distilled water. The seaweed complex
consisting of
Ulva lactuta, Astaxanthin, Alteromonas maclodeii (source: Unipex Innovations;
Quebec
City, Quebec) was dissolved into one half of the polarized water. The mixture
was
maintained at approximately 85 degrees Celsius. While maintaining the
temperature, the
mixture was stirred for approximately 45 minutes to 60 minutes. Thereafter,
beta gluean
(source: Biothera) and fucoidan (source: Marinova) complex were dissolved in
the
seaweed complex-distilled water mixture. This mixture was maintained at
approximately
85 degrees Celsius and the mixture was stirred for at least approximately 30
minutes.
Thereafter, while maintaining the mixing of the mixture, the aqueous Aloe
barbedensis leaf extract (source: Tri K Industries) was added and the entire
mixture was
allowed to cool for approximately 30 minutes.
Therafter, hydrolyzed pearl nacre (source: Rita Corp.; Crystal Lake, Illinois)
and
chitosan (source: Alfa Chem), acetyl glucosomine (source: Alfa Chem), n-acetyl
carnatine
(source: Alfa Chem), alpha lipoic acid (source: Alfa Chem), and 1-arginine
(source: Alfa
Chem) were added to the cooled mixture. The cooled mixture was further cooled
to
approximately 30 degrees Celsius. Thereafter, phospholipids (source: Arch
Personal
Care) and sodium hyaluronate (source: Tri-K Industries) were added to the
mixture.
CA 02701378 2010-04-22
-18-
Table 2. Components of an embodiment of the invention
Sequence No. Component Amount elk
by welaht)
Distilled water 18.8
Tourmaline (0.15%) and volcanic obsidian (0.05%) 0.2
Seaweed complex containing Ulva lactuta, 10
Astaxanthin, Alteromonas maclodeii (ratio of these
products: 7:1.5:1.5)
Beta glucan (yeast and specified medicinal fungi 10
extract combined in a 1:1 ratio) and fucoidan complex
(beta glucan and fucoidan are present in a 1:1 ratio)
Aqueous Aloe barbedensis leaf extract 12
Hydrolyzed pearl nacre and chitosan (ratio of these 1.5
products: 1.5:1)
N-acetyl carnitine, alpha lipoic acid, and I-arginine 3.5
(ratio of these products: 1.5:1.5:0.5)
Phospholipids and sodium hyaluronate (ratio of these 8
products: 4:1)
II Glyceryl stearate, stearic acid, cetearyl alcohol, 15
ceteareth 20, isopropyl palmitate complex (each of
these products is added at an equal amount)
II Ascorbyl polypeptide, tocotrienol, tocopheryl acetate, 5
ergocalciferol, ferulic acid, niacinamide complex (ratio
of these products: 3:1:0.5:0.5:0.5:0.5)
Glycine sofa, Rumex crispis, Vitis vinifera, hinokitiol 2
(beta thujaplicin) (each of these products is added at
an equal amount)
II Aqueous extract of Camellia sinensis, CenteHa 2.5
asiatica, Chamomile matricaria, Echinacea augustifolia,
Ginko biloba (each of these products is added at an
equal amount)
Ill Complex of butylene glycol, phenoxyethanol, caprylyl 3.5
glycol, sorbic acid, peg8/SMDI copolymer (ratio of
these products: 1.5:.05:0.25:0.25:0.25)
Ill Aqueous superoxide dismutase liposomes 8
CA 02701378 2010-04-22
-19-
Method Sequence No. 2
In a second mixture, glyceryl stearate, stearic acid, cetearyl alcohol,
ceteareth 20,
isopropyl palmitate complex (all from source: Hallstar Corp.) were heated to
75 degrees
Celsius in a 200 kg. stainless steel vat. The mixture was mixed by continuous
stirring for
approximately 60 minutes.
Separately, ascorbyl polypeptide, tocotrienol, tocopheryl acetate,
ergocalciferol,
ferulic acid, and niacinamide (all from source: Arch Personal Care) were
dissolved into
the second half of the polarized water (as described above) over a period of
approximately
one hour. Thereafter, Glycine sofa, Rumex crispis, Vitis vinifera, and
hinokitiol (beta
thujaplicin) (all from source: Active Organics); and aqueous extracts of
Camellia sinensis,
Centella asiatica, Chamomile matricaria, Echinacea augustifolia, and Ginko
biloba (all
from source: Active Organics) were added to the ascorbyl polypeptide-
tocotrienol mixture.
Method Sequence No. 3
Butylene glycol, phenoxyethanol, eapryly1 glycol, sorbic acid and peg8/SMDI
copolymer
(all from source: International Specialty Products) were combined to form a
complex.
Thereafter, the complex was added to the glyceryl stearate mixture described
above, while
the glyceryl stearate mixture was maintained at approximately 75 degrees
Celsius.
Thereafter, the ascorbyl polypeptide-tocotrienol mixture described above was
added and
agitation of the mixture continued at approximately 75 degrees Celsius. The
entire
mixture was then cooled to approximately 30 degrees Celsius. Thereafter, the
mixture
developed above in Method Sequence No. 1 was added. Thereafter, the mixture
was
allowed to cool to approximately 20 degrees Celsius and liposomal superoxide
dismutase
(source: Arch Personal Care) was added. The mixture developed in Example 2 is
a
homogenous mixture.
In a modification to the above, the seaweed complex can be present as 4% of
the
formulation instead of 10% as described in Table 2. Further, the beta glucan
and fucoidan
complex can be present as 16% of the formulation instead of 10% as described
in Table 2.
CA 02701378 2010-04-22
-20-
Testing efficacy of Example 2
To test the efficacy of the mixture described in Example 2, the mixture was
used
by human subjects to determine the effect on the appearance of their skin. The
results
routinely showed that the mixture described in Example 2 improved the
appearance of the
skin of human subjects.
Serving as an example, MJ, a female over the age of 40 years of age, used the
formulation defined in Example 2. Prior to using the formulation of Example 2,
MJ had
exhibited under-eye skin that was puffy, sensitive and quite dry. On day 0, MJ
began
using an amount approximately equivalent to the size of a dime and used
approximately
that amount each morning and night on her skin. After one week, MJ described
her under-
eye skin as being less puffy. After two weeks, MJ again described her under-
eye skin as
being less puffy. After one month, MJ described her under-eye skin as bein
tighter
looking and having fewer small wrinldes such that the skin appeared to be
smoother in
appearance. MJ identified at least the following attributes as being
associated with the
formulation defined in Example 2: decreased skin wrinldes; improvement in skin
tone;
increased skin firmness; and increased skin hydration.
It was determined that the formulation/mixture of Example 2 was absorbed by
human skin.
Example 3. Composition with acidified fucoidan, beta glucan and retinol
In accordance with a further embodiment of the present invention, a
composition is
described having the components shown in Table 3. The methodology employed in
producing the composition using the amounts described in Table 3 is generally
as follows.
Method Sequence No. 1
In a first mixture, neutralized glycolic and salicylic acid solution (source:
Dupont
or Spectrum Chemical) was heated in a stainless steel container to
approximately 85
degrees Celsius. The seaweed complex consisting of
CA 02701378 2010-04-22
-21-
Table 3. Components of an embodiment of the invention
Sequence No. Component Amount (%
by weight)
Glycolic and salicylic acids (ratio: 8:1) (neutralized 10
with ammonium hydroxide; ammonium glycolate,
and sodium lactate can also be added to obtain a
preferable pH of ¨4)
Seaweed complex containing Eckionia cava, 5.5
Alteromonas maclodeii and Astaxanthin [ratio:
7:1.5:1.5]
Distilled water 14.8
Tourmaline (0.15%) and volcanic obsidian (0.5%) 0.2
Beta glucan (yeast and specified medicinal fungi 8
extract combined in a 1:1 ratio) in a 1:1 ratio with
fucoidan complex
Aqueous Aloe barbedensis leaf extract 10
Hydrolyzed pearl nacre (1.5%) and chitosan (1.0%) 2.5
Phospholipids 10
Glyceryl stearate, stearic acid, cetearyl alcohol, 20
ceteareth 20, isopropyl palmitate (each of these
products is added at an equal amount)
Tocotrienol, tocopheryl acetate, niacinamide, retinol 6
complex (ratio of these products: 3:1.5:1.5:0.5)
Aqueous extract of Vitis vinifera, Chamomile 2.5
matricaria, Echinacea augustifolia, Panax ginseng,
Glycine sofa, allantoin (each of these products is
added at an equal amount)
Ill Complex of butylene glycol, phenoxyethanol, 2.5
caprylyl glycol, sorbic acid, peg8/SMDI copolymer
(ratio of these products: 1.5:0.5:0.25:0.25:0.25)
Ill Aqueous superoxide dismutase liposomes 8
CA 02701378 2010-04-22
-22-
EckIonia cava, Alteromonas maclodeii, and Astaxanthin (source: Unipex
Innovations) was
added to the heated glycolic acid mixture. The combined mixture was mixed for
approximately 45 to 60 minutes.
Polarized water was developed by adding tourmaline and obsidian (source:
Soliance) to the distilled water. The beta glucan and fucoidan complex
(source: Active
Concepts, Marinova) was dissolved in one-third of the polarized water. The
dissolved
beta glucan - fucoidan complex was added to the glycolic acid mixture
(previously
described above) and the entire solution continued to be stirred while the
temperature was
maintained at approximately 85 degrees Celsius for approximately 30 minutes.
While
maintaining mixing of the mixture, aqueous Aloe barbedensis leaf extract
(source: Tri K
Industries) was added and the entire mixture was allowed to cool for 30
minutes.
Thereafter, hydrolyzed pearl nacre (source: Rita Corp.) and chitosan (source:
Alfa Chem)
were added to the cooled mixture. The entire mixture was allowed to cool to
approximately 30 degrees Celsius. Thereafter, phospholipids (source: Arch
Personal
Care) were added to the cooled mixture.
Method Sequence No. 2
In a further mixture, glyceryl stearate, stearic acid, cetearyl alcohol,
ceteareth 20,
and isopropyl palmitate complex (source: Hallstar Corp.) were heated to 75
degrees
Celsius in a 200 kg. stainless steel vat. The mixture was mixed by continuous
stirring for
60 minutes.
Separately, the remaining two-thirds of polarized water (as described above)
was
used to dissolve the tocotrienol - retinol complex (source: Arch Personal
Care). The
tocotrienol - retinol complex was allowed to dissolve in the polarized water
for one hour.
Thereafter, aqueous extracts of Vitis vinifera, Chamomile matricaria,
Echinacea
augustifolia, Panax ginseng, Glycine sofa, and allantoin (source: Active
Organics) were
added to the tocotrienol - retinol complex.
Method Sequence No. 3
In a further mixture, butylene glycol, phenoxyethanol, caprylyl glycol, sorbic
acid,
and peg8/SMDI copolymer were combined to make a complex (source: International
Specialty Products). Thereafter, this complex was added to the glyceryl
stearate complex
CA 02701378 2010-04-22
-23-
mixture described above, the glyceryl stearate complex having been maintained
at 75
degrees Celsius. Thereafter, the aseorbyl polypeptide-tocotrienol complex,
which had
previously been mixed with the aqueous extracts described above, was added and
agitation
of the mixture continued at approximately 75 degrees Celsius. Thereafter, the
entire
mixture was cooled down to approximately 30 degrees Celsius.
Thereafter, the mixture developed from Method Sequence No. 1 was added to the
mixture. The mixture was allowed to cool down to approximately 20 degrees
Celsius and
liposomal superoxide dismutase (source: Arch Personal Care) was added. The
mixture
developed in Example 3 is a homogenous mixture.
In a modification to the above, the seaweed complex can be present as 3.5% of
the
formulation instead of 5.5% as described in Table 3. Further, the beta glucan
and fucoidan
complex can be present as 10% of the formulation instead of 8% as described in
Table 3.
Testing efficacy of Example 3
To test the efficacy of the mixture described in Example 3, the mixture was
used
by human subjects to determine the effect on the appearance of their skin. The
results
routinely showed that the mixture described in Example 3 improved the
appearance of the
skin of human subjects.
Serving as an example, CO, a female between the age of 30-40 years of age,
used
the formulation defined in Example 3. Prior to using the formulation of
Example 3, CO
had exhibited oily skin that was prone to acne breakouts. Further, CO
described that her
skin was rough, and that skin pores were visible. Further, CO described that
her skin
showed an appearance of acne scarring. On day 0, CO began using an amount
approximately equivalent to the size of a dime and used approximately that
amount each
night on her skin. After one week, CO described her skin as having faded acne
scarring.
After two weeks, CO described that her skin appeared to be more radiant and
glowing in
appearance. After one month, CO described her skin as being firmer and that
the acne
breakouts had stopped. CO identified at least the following attributes as
being associated
with the formulation defined in Example 3: decreased skin wrinkles;
improvement in skin
tone; increased skin firmness; and increased skin hydration.
It was determined that the formulation/mixture of Example 3 was absorbed by
human skin.
CA 02701378 2010-04-22
-24-
Example 4. Composition with acidified fucoidan and beta glucan
In accordance with a further embodiment of the present invention, a
composition is
described having the components shown in Table 4.
Table 4. Components of an embodiment of the invention
Sequence No. Component Amount Pk
by weiaht)
Aqueous Aloe barbedensis leaf extract 45
Distilled water 40.8
Tourmaline, volcanic obsidian 0.2
Glycolic and salicylic acids (ratio 8:1) (neutralized 6
with ammonium hydroxide; ammonium glycolate,
and sodium lactate can also be added to obtain a
preferable pH of -4)
Fucoidan, beta glucan, Ulva lactuta (ratio: 5:5:1.5) 4
II Aqueous extract of Came/ha sinensis, Centella 1.5
asiatica, Chamomile matricaria, Echinacea
augustifolia, Ginko biloba (these extracts are present
in equal amounts)
Ill Complex of butylene glycol,
allantoin, 2.5
phenoxyethanol, caprylyl glycol, sorbic acid (ratio:
1.5:0.5:0.25:0.25:0.25)
The methodology employed in producing the composition using the amounts
described in Table 4 is generally as follows.
Tourmaline and volcanic obsidian (source: Soliance) were dissolved into
distilled
water. Ulva lactuta, fucoidan (source: Marinova), beta glucan (source: Arch
Personal
Care) were added to above polarized water and mixture was heated to
approximately 80
degrees Celsius and stirred for approximately 40 minutes.
Neutralized glycolic acid, as disclosed herein, was added to the above
mixture, and
stirred at approximately 80 degrees Celsius for approximately another 30
minutes.
Thereafter, Aloe barbedensis leaf extract (source: Tri-K) and aqueous extract
of Camellia
sinensis, Centella asiatica, Chamomile matricaria, Echinacea augustifolia, and
Ginko
CA 02701378 2010-04-22
-25-
biloba (source: Active Organics) as detailed in the corresponding Table, were
added and
the entire mixture was stirred for approximately another 10 minutes.
At this stage any of a variety of surfactants can be incorporated to
constitute an
effective cleansing composition focusing on the above key ingredients.
Thereafter, butylene glycol, allantoin, phenoxyethanol, caprylyl glycol,
sorbic acid
(source: International Specialty Products) were added and the entire mixture
was stirred
for approximately another 10 minutes. The entire mixture was then cooled to
room
temperature. The mixture developed in Example 4 is a homogenous mixture.
Testing efficacy of Example 4
To test the efficacy of the mixture described in Example 4, the mixture was
used
by human subjects to determine the effect on the appearance of their skin. The
results
routinely showed that the mixture described in Example 4 improved the
appearance of the
skin of human subjects.
Serving as an example, MT, a male under the age of 30 used the formulation
defined in Example 4. Prior to using the formulation of Example 4, MT had
exhibited
slightly acned skin that included a small amount of wrinkling. On day 0, MT
began
applying the formulation defined in Example 4 to his skin using a cotton pad
soaked in the
formulation defined in Example 4. After one week, MT described his skin as
appearing
clearer with a less dull appearance. After two weeks, MT described his skin as
having less
breakouts of acne. After one month, MT described his skin as appearing
brighter, clearer,
and tighter. MT identified at least the following attributes as being
associated with the
formulation defined in Example 4: increased skin firmness; improved skin tone,
and
smoother and brighter skin appearance.
It was determined that the formulation/mixture of Example 4 was absorbed by
human skin.
Example 5. Composition with acklUied fucoidan
In accordance with a further embodiment of the present invention, a
composition is
described having the components shown in Table 5.
CA 02701378 2010-04-22
-26-
Table 5. Components of an embodiment of the invention
Sequence No. Component Amount (70
by weight)
Glycolic and salicylic acids (ratio 8:1) (neutralized 12.8
with ammonium hydroxide; ammonium glycolate,
and sodium lactate can also be added to obtain a
preferable pH of -4)
Tourmaline (0.15%) and volcanic obsidian (0.5%) 0.2
Kaolin and bentonite (ratio of these products: 26
0.6:0.3)
Beta glucan (yeast and specified medicinal fungi 10
extract combined in a 1:1 ratio) in a 1:1 ratio with
fucoidan complex, Ulva lactuta [5:5:1.5]
Undaria pinnatifida 4
Diatomite silica, n-acetyl glucosamine, hydrolyzed 2.5
pearl nacre, and chitosan (ratio of these products:
1:1:0.25:0.25)
Aqueous Aloe barbedensis leaf extract 10
Echinacea augustifolia and Panax ginseng (ratio of 2
these products: 1:1)
Stearic acid, cetearyl alcohol, and ceteareth 20 30
complex in equal parts (each of these products is
added at an equal amount)
III Complex of phenoxyethanol, caprylyl glycol, sorbic 2.5
acid (ratio of these products: 1.5:0.5:0.5)
CA 02701378 2010-04-22
-27-
The methodology employed in producing the composition using the amounts
described in Table 5 is generally as follows.
Method Sequence No. /
In a first mixture, neutralized glycolic acid (source: Dupont or Spectrum
Chemical), salicylic acid (source: Spectrum), tourmaline and volcanic obsidian
(source:
SoHance) was heated in a stainless steel container to approximately 85 degrees
Celsius.
The seaweed complex consisting of ulva lactuta (source: Unipex Innovations),
beta glucan
fucoidan complex (source: Biothera, Marinova), and Undaria pinnatifida
(source:
Marinova) were added to the heated glycolic acid mixture. The combined mixture
was
agitated for approximately 30 minutes. Diatomite silica, n-acetyl glucosamine,
hydrolyzed
pearl nacre (source: Rita Corp.), and chitosan (source: Alfa Chem) were added
to the
above mixture.
Kaolin and bentonite (source: Xenex Labs; Vancouver, British Columbia] were
separately mixed and added to the above mixture. While maintaining mixing of
the
mixture, aqueous Aloe barbedensis leaf extract (source: Tri K Industries),
Echinacea
augustifolia, and Panax ginseng (source: Active Organics) were added.
Method Sequence No. 2
Thereafter, and separately, stearic acid, cetearyl alcohol, and ceteareth 20
complex
(source: Hallstar Corp.) were heated to approximately 75 degrees C, stirring
for
approximately 60 minutes.
Method Sequence No. 3
In a further mixture, phenoxyethanol, caprylyl glycol, and sorbic acid
(source:
International Specialty Products) were combined to make a complex. Thereafter,
this
complex was added to the stearic acid complex described above, the stearic
complex
having been maintained at approximately 75 degrees Celsius. Thereafter, the
entire
mixture was cooled down to approximately 30 degrees Celsius. Thereafter, the
mixture
developed from Method Sequence No. 1 (see above) was added to the mixture. The
mixture was allowed to cool to approximately 20 degrees Celsius. The mixture
developed
in Example 5 is a homogenous mixture.
CA 02701378 2010-04-22
In a modification to the above, the beta glucan complex can be present as 12%
of
the formulation instead of 10% as described in Table 5. Further, the Undaria
pinnatifida
can be present at 2% instead of 4% as described in Table 5.
Testing efficacy of Example 5
To test the efficacy of the mixture described in Example 5, the mixture was
used
by human subjects to determine the effect on the appearance of their skin. The
results
routinely showed that the mixture described in Example 5 improved the
appearance of the
skin of human subjects.
Serving as an example, JT, a female between the age of 30-40, used the
formulation defined in Example 5. Prior to using the fotinulation of Example
5, JT had
exhibited skin conditions that included acne and rough spots. On day 0, JT
began using an
amount approximately equivalent to the size of a quarter and used
approximately twice
that amount twice a week. After one week, JT described her skin as having
fewer
breakouts of acne. After two weeks, JT described her skin as being clearer.
After one
month, JT described her skin as appearing smoother. JT identified at least the
following
attributes as being associated with the formulation defined in Example 5:
increased
smoothness of the skin, increased luminosity of the skin, increased firmness
of the skin,
increased tautness of the skin, and less irritation and skin-associated
inflammation.
It was determined that the formulation/mixture of Example 5 was absorbed by
human skin.
Example 6. Serum composition
The methodology employed in producing the non-acidified serum composition
using the amounts described in Table 6 is generally as follows.
Method Sequence No. 1
Polarized water was developed by adding tourmaline and obsidian (source:
Soliance) to the distilled water. The seaweed complex consisting of
phytoplankton
extract (source: Canadian Pacific Phytoplankton), Fucus vesiculosus extract
(source:
Xenex), ecklonia cava (source: JP Renew; San Francisco, California) was
dissolved into
one-half of the distilled water, heated to approximately 85 degrees Celsius.
The combined
CA 02701378 2010-04-22
-29-
mixture was then stirred for approximately 45 minutes to 60 minutes. The beta
glucan and
fucoidan complex (source: Biothera, Marinova) and vegetal astaxanthin (source:
Unipex
Innovations) was dissolved in the seaweed complex-distilled water mixture
(previously
described above). The heated mixture continued to
Table 6. Components of an embodiment of the invention
SEQUENCE INGREDIENT AMOUNT
Distilled water 40.8
Tourmaline, volcanic obsidian [1 0.2
1 Phytoplankton extract, Fucus vesiculosus extract, 6
EckIonia cava complex [1:1:.5:.5]
Beta glucan (yeast and specified medicinal fungi 8
extract combined in a 1:1 ratio), fucoidan, vegetal
astaxanthin complex [5:5:1.5]
Hydrolyzed pearl nacre, chitosan [1:.5] 1.5
1 Phospholipids, sodium pCA [1:.5] 8
II Magnesium ascorbyl phosphate, tocotrienol, 25
niacinamide, tocopheryl acetate, ergocalciferol
complex [10:1:.5:.5:.5]
II CameNa sinensis, epigallocatechin (EGCG), ellagic 4
acid, 1-arginine [2:1:.5:.5]
Ill Complex of butylene glycol, phenoxyethanol, caprylyl 3.5
glycol, sorbic acid, peg8/SMDI copolymer
[2:1 : .5: .5: .5]
Ill Sodium citrate, tetrasodium EDTA [1:.5] 3
be stirred while the temperature was maintained at approximately 85 degrees
Celsius for a
minimum of approximately 30 minutes. The entire mixture was allowed to cool to
approximately 30 degrees Celsius. Thereafter, hydrolyzed pearl nacre (source:
Active
Concepts) and chitosan (source: Alfa Chem) were added to the cooled mixture.
Thereafter,
phospholipids (source: Arch Personal Care) and sodium hyaluronate (source: Tri-
K
Industries) were added to the cooled mixture.
CA 02701378 2010-04-22
-30-
Method Sequence No. 2:
In a further mixture, a complex comprising magnesium ascorbyl phosphate
(source: Optima Specialty; Huntington, Connecticut) with tocotrienol,
niacinamide,
tocopheryl acetate, ergocalciferol (source: Arch Personal Care) was mixed into
the
remaining one-half of polarized water and heated to approximately 70 degrees
Celsius.
This mixture was allowed to dissolve in the polarized water for one hour.
Thereafter,
Camellia sinensis (source: Active Organics), epigallocatechin (EGCG) (source:
DSM
Nutritional Products; Belvedere, New Jersey), ellagic acid (source: DSM), and
1-arginine
(source: Alpha Chem) were added to the ascorbyl polypeptide ¨ tocotrienol
complex.
Method Sequence No. 3
In a further mixture, butylene glycol, phenoxyethanol, caprylyl glycol, sorbic
acid, and
peg8/SMDI copolymer (source: International Specialty Products) are combined in
the ratio
specified in the Table 6 above to make a complex. Thereafter, this complex was
added to
the magnesium ascorbyl ¨ tocotrienol mixture described above in Method
Sequence No. 2,
the magnesium ascorbyl ¨ tocotrienol having been maintained at approximately
70 degrees
Celsius. Thereafter sodium citrate and tetrasodium EDTA (source: Xenex) was
added.
The mixture was then stirred for approximately 15 minutes, while maintaining
at
approximately 70 degrees Celsius. Thereafter, the entire mixture was cooled
down to
approximately 30 degrees Celsius. Thereafter, the mixture developed from
Method
Sequence No. 1 was added to the mixture. The mixture was allowed to cool down
to
approximately 20 degrees Celsius. The mixture developed in Example 6 is a
homogenous
mixture.
In a modification to the above, the phytoplankton extract can be present as 2%
of
the formulation instead of 6% as described in Table 6. Further, the bet glucan
complex can
be present at 12% instead of 8% as described in Table 6.
Testing efficacy of Example 6
To test the efficacy of the mixture described in Example 6, the mixture was
used
by human subjects to determine the effect on the appearance of their skin. The
results
routinely showed that the mixture described in Example 6 improved the
appearance of the
skin of human subjects.
CA 02701378 2010-04-22
-3 1 -
Serving as an example, TK, a female over the age of 40 used the formulation
defined in Example 6. Prior to using the formulation of Example 6, TK had
exhibited
sensitive skin conditions and her skin was sun-damaged. On day 0, TK began
using an
amount equivalent to the size of a dime of the formulation in Example 6 on a
daily basis.
After one week, TK described her skin as appearing more refreshed. After two
weeks, TK
described her skin as appearing less irritated or red as compared with before
use of the
formulation. After one month, TK described her skin as appearing clearer, and
more firm
with less wrinkle lines. TK identified at least the following attributes as
being associated
with the formulation defined in Example 6: increased smoothness (diminished
appearance
of wrinldes); increased luminosity; increased firmness; increased tautness;
and less
irritation and skin-associated inflammation.
It was determined that the formulation/mixture of Example 6 was absorbed by
human skin.
While specific embodiments of the invention have been described and
illustrated,
such embodiments should be considered illustrative of the invention only and
not as
limiting the invention as construed in accordance with the accompanying
claims.
