Note : Les descriptions sont présentées dans la langue officielle dans laquelle elles ont été soumises.
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Orodispersible composition comprising polyunsaturated fatty acids without bad
odour or
taste
The present invention relates to an orodispersible composition without bad
odour, smell or taste
comprising polyunsaturated fatty acids (PUFA), its process for preparation and
its use as
nutritional supplement or as dietary supplement for balancing the blood lipid
level, preventing or
reducing the risk of the development of atherosclerotic changes, disorders or
diseases.
Polyunsaturated fatty acids (PUFA) are the active ingredients e.g. in fish oil
and are responsible
for significant low blood lipid levels and low incidence of hypertension which
was shown in a
epidemiological study with Inuits (M.H. Davidson, P. R. Liebson,
Cardiovascular
Reviews&Reports 1986, 7, 461-472). In particular the blood concentration of
the low density
lipoprotein cholesterol (LDL) is lowered and that of the high density
lipoprotein cholesterol
(HDL) is increased. Coronary heart disease (CHD) is the major cause of death
in the western
countries and high plasma cholesterol levels, more specifically the LDL/HDL
ratio, is highly
correlated with the risk of CHD (Willett and Sacks, N. Eng. J. Med. 1991, 121,
324).
The PUFA found in fish oil have carbon chains of 18, 20 or 22 atoms and can be
classified in n-3
omega and n-6 omega fatty acids which are essential for the human body. In
particular the omega-3
fatty acids eicosapentenoic acid (EPA) and docosahexenoic acid (DHA) are only
found in fish and
other marine life. Fish oil is therefore the most important food source for
omega-3 fatty acids.
Standard nutritional supplement of PUFA is achieved by a daily administration
of 500-1000 mg
liquid fish oil. The fish oil is normally administered in capsules in order to
prevent the fishy smell
and odour. Nevertheless some people experience gastrointestinal upset of the
fishy smell even
hours after the fish oil is taken. The reason is that after decomposition of
the galantine wall in the
gastro-intestinal tract, the voluminous dosage of the fish oil is released as
a macroscopic drop
which interfere with the resorption of the fish oil. The problems of bad
smell, odour and resorption
can be solved in part by microdispersed fish oil preparations as pulverulent
or aqueous matrix as
described in EP 0 276 772. However, even the microdispersed fish oil granule
or powder has a
remaining fishy smell when pressed in simple tablets, so that flavours or
antioxidants are needed to
be added to the formulations.
The flavours are used to domineer over the fishy smell of the fish oil as
described e.g. in JP
08092587 or JP 08228678. Taste masking is also achieved by adding milk
products to the fish oil
formulation as described e.g. in US 2003/0198728 or JP 2002-204656 or by
coating or
encapsulating the PUFA as described e.g. in WO 2004/016720 and WO 2005/029978.
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=
The reason for the fishy smell of the fish oil is based on the oxidation of
the unsaturated part of the
PUFA. In order to prevent oxidation and to stabilize the PUFA antioxidants
e.g. tocopherol can be
added to the formulation as described e.g. in DE 20105126 or EP 1 155 620.
Surprisingly it is found that a bad or fishy smell of the composition
according to the present
invention can be avoided.
Subject of the present invention is an orodispersible composition comprising
i) a fish oil, a perilla oil or a polyunsaturated fatty acids
(PUFA); and
ii) a
disintegrant which is a solid dispersion comprising mannitol, xylitol,
microcrystalline.
cellulose, crospovidone and dibasic calcium phosphate.
The oral composition according to the invention has not a fishy or bad smell,
odour or taste.
According to the present invention polyunsaturated fatty acids (PUFA) include,
but are not limited
to, fish oil, perilla oil, omega-3 fatty acids, omega-6-fatty acids,
arachidonic acid, linoleic acid,
= 15 alpha-linoleic acid, dihomogammalinoleic acid, eicosapentenoic
acid (EPA), docosahexenoic acid
(DHA) and mixtures thereof. Preference is given to fish oil, omega-3 fatty
acids, eicosapentenoic
acid (EPA), docosahexenoic acid (DHA) and mixtures thereof. Most preferably
fish oil containing
eicosapentenoic acid (EPA) and docosahexenoic acid (DHA) is used.
In a preferred embodiment the composition according to the invention comprises
PUFA, in
particular fish oil powder or granulate, in an amount of 5 % to 70%, more
preferable 40 % to 60 %
by weight of the composition. The total amount of the PUFA, in particular fish
oil powder or
granulate, used in the composition is 100 mg- 1000 mg, preferably 400 mg - 900
mg.
In a most preferred embodiment the composition according to the invention
comprises
eicosapentenoic acid (EPA) in an amount of 0.5 % to 10 %, preferably 1.5 % to
4 % by weight of
the composition or in an amount of I % to 20 %, preferably 3 % to 8 % by
weight of the fish oil
powder or granulate contained in the composition. The total amount of EPA in
the composition is
10 mg ¨ 100 mg, preferably 15 mg¨SO mg.
=
In a most preferred embodiment the composition according to the invention
comprises
docosahexenoic acid (DHA) in an amount of 0.5 % to 10 %, preferably 1 % to 4 %
by weight of
the composition or in an amount of 1 % to 20 %, preferably 2 % to 6 % by
weight of the fish oil
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powder or granulate contained in the composition. The total amount of DHA in
the composition is
mg ¨50 mg, preferably 15 mg ¨30 mg.
The PUFA, in particular the fish oil containing eicosapentenoic acid (EPA) and
docosahexenoic
acid (DHA), is used preferably in a microdispersed form as a granulate or
powder, in a pulverulent
5 or aqueous matrix as described in EP 0 276 772. Preference is given to a
fish oil granulate or
powder.
In a preferred embodiment the pulverulant matrix of the fish oil powder or
granulate comprises at
least a homogenous protective colloid, a surfactant, optionally a diluent,
stabilizer and further
pharmaceutical excipients. In the case of the aqueous matrix the mentioned
ingredients are used as
10 an aqueous solution.
The protective colloids of the pulverulant matrix include but are not limited
to polypeptides such
as gelatine, casein, caseinate, polysaccharides such as starch, dextrin,
pectin, Arabic gum, milk,
milk powder, polyvinyl alcohols, polyvinylpyrrolidone, vinylpyrrolidone-
vinylacetate-copolymers,
acrylic acid- and methacrylic acid-copolymers with acrylic acid- or
methacrylic acid esters, methyl
cellulose, carboxymethyl cellulose, hydroxypropyl cellulose, alginates or
mixtures thereof.
Diluents of the pulverulant matrix include but are not limited to sugar or
sugar alcohols e.g.
saccharose, lactose, invert sugar, sorbit, mannit or glycerine.
Stabilizers of the pulverulant matrix include but are not limited to
tocopherol, t-butyl
hydroxytoluol, t-butyl hydroxyanisol and ethoxyquine.
Surfactants of the pulverulant matrix include but are not limited to esters of
long chain fatty acids
and ascorbic acid, esters of mono- and diglycerin and fatty acids and
oxethylated derivatives
thereof, esters of mono fatty acid glycerides with acetic acid, citric acid,
lactic acid,
diacetyltartrate, salts of 2-(2'-stearoyllactyl) lactic acid, polyglycerine
fatty acid esters, sorbitan
fatty acid esters, porpylenglycol-fatty acid esters, ascorbylpalmitate and
lecithin.
The pulverulant matrix comprises fish oil in the amount of 5 % to 70 %,
preferably 50 % to 60 %,
one or more surfactants in an amount of 1 % to 30 %, preferably 5 % to 15 %, a
protective colloid
in an amount of 5 % to 50 %, preferably 10 % to 40 %, a diluent in an amount
of 0 % to 70 %,
preferably 3 % to 35 %, by weight of the dry mass of the fish oil powder or
granulate.
In a preferred embodiment the pulverulant matrix comprises the fish oil in
small particles having
an average particle size of less then 10 m, more preferably less then 1 p.m,
most preferably less
then 0.5 p.m.
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The fish oil powder or granulate can be prepared as described in EP 0 276 772.
According to the present invention the solid dispersion responsible for rapid
disintegration
comprises mannitol, xylitol, microcrystalline cellulose, crospovidone and
dibasic calcium
phosphate wherein microcrystalline cellulose, crospovidone and dibasic calcium
phosphate are
dispersed in the mixture of mannitol and xylitol. The solid dispersion
according to the invention
can be prepared as described in EP 1 523 974.
The amount of mannitol and xylitol in the solid dispersion is from 40 % to 90
%, preferably from
50 % to 80 %, more preferably from 60 % to 78 %, and most preferably from 62 %
to 78 % by
weight of the solid dispersion, and the amount of mannitol and xylitol in the
total composition is
from 18 % to 41 %, preferably from 23 % to 36 %, more preferably from 27 % to
35 % , and most
preferably from 28 % to 35 % by weight of the total composition.
The ratio by weight of mannitol and xylitol is from (98 to 67) : (2 to 33),
preferably from (98 to
87) : (2 to 13), more preferably from (97 to 87) : (3 to 13), and most
preferably from (96 to 89) : (4
to 11).
The amount of dibasic calcium phosphate (which corresponds to calcium
monohydrogen
phosphate) in the solid dispersion is from 1 % to 30 %, preferably 2 % to 15
%, and more
preferably 3 % to 8 % by weight of the solid dispersion, and the amount of
dibasic calcium
phosphate in the total composition is from 0.4 % to 13 %, preferably 1 % to 7
%, and more
preferably 2 % to 4 % by weight of the total composition.
The amount of microcrystalline cellulose in the solid dispersion is from 8 %
to 22 %, preferably
from 10 % to 22 %, more preferably from 12 % to 21 % by weight of the solid
dispersion, and the
amount of microcrystalline cellulose in the total composition is from 3 % to
10 %, preferably from
4 % to 10 %, more preferably from 5 % to 9 % by weight of the total
composition.
The amount of crospovidone in the solid dispersion is from 5 % to 15 %,
preferably from 5 % to
14 %, more preferably from 6 % to 13 % by weight of the solid dispersion, and
the amount of
crospovidone in the total composition is from 2 % to 7 %, preferably from 2 %
to 6 %, more
preferably from 3 % to 6 % by weight of the total composition.
In a preferred embodiment D-mannitol is used in the solid dispersion.
The composition according to the invention can comprise further active
ingredients such as
vitamins and minerals. Vitamins include, but are not limited to, vitamin A,
beta carotene, vitamin
C (ascorbic acid), vitamin D3 (cholecalcipherol), vitamin E (tocopherol
acetate), vitamin B1
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(thiamine), vitamin B2 (riboflavin), nicotinamide, vitamin B5 (panthothenic
acid), vitamin B6
(pyridoxine), folic acid, vitamin B12 (cyanocobalamin), vitamin K 1, vitamin
K2, especially
menaquinone 7-10, and biotin. Minerals include, but are not limited to, iron
salts, copper salts,
calcium salts such as calcium carbonate, calcium phosphate, calcium
glycerophosphate;
magnesium salts such as magnesium phosphate, magnesium sulphate (dihydrate) or
magnesium
oxide; zinc salts such as zinc citrate; selenium salts such as sodium
selenate; potassium iodide;
manganese salts such as manganese sulphate; molybdate salts such as sodium
molybdate;
chromium salts such as chromium chloride; sodium chloride and potassium
chloride.
The composition according to the present invention can be used as nutritional
supplement or as
dietary supplement for balancing the blood lipid level, preventing or reducing
the risk of the
development of atherosclerotic changes, disorders or diseases in a patient.
The inventive
composition can also be used as nutritional or as dietary supplement for
developing and
maintaining the cognitive functions connected with e.g. eyes, memory, language
etc. or for
alleviating and/or preventing blood vessel diseases, cardiovascular,
cerebrovascular and nervous
diseases such as e.g. hypertension, cardiac infarction, Alzheimer, Parkinson
and depression, or for
alleviating hormonal, immunologic disorders or obesity. Furthermore, it can be
used as nutritional
or dietary supplement to support treatments of diabetes, cancer and/or
inflammatory affections. A
patient, for the purpose of this invention, is a mammal, including a human.
The use as a nutritional
or dietary supplement is especially preferred for pregnant women, children and
elderly persons.
A further aspect of the invention is a method for balancing the blood lipid
level, preventing or
reducing the risk of the development of atherosclerotic changes, disorders or
diseases, for
developing and maintaining the cognitive functions connected with e.g. eyes,
memory, language
etc., for alleviating and/or preventing blood vessel diseases, cardiovascular,
cerebrovascular and
nervous diseases such as e.g. hypertension, cardiac infarction, Alzheimer,
Parkinson and
depression, or for alleviating hormonal, immunologic disorders or obesity or
for supporting
treatments of diabetes, cancer and/or inflammatory affections by administering
the inventive
composition as nutritional supplement or as dietary supplement to a patient
which is, for the
purpose of this invention, a mammal, including a human, especially pregnant
women, children and
elderly persons.
The composition according to the invention is administered orally one or more,
preferably up to
three, more preferably up to two times per day. With each administration the
number of dosage
forms taken in at the same time should not exceed two.
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Nevertheless, it may in some cases be advantageous to deviate from the amounts
specified,
depending on body weight, individual behaviour toward the active ingredient,
type of preparation
and time or interval over which the administration is effected. For instance,
less than the
aforementioned minimum amounts may be sufficient in some cases, while the
upper limit specified
has to be exceeded in other cases.
Ingredients of the oral dosage form are those which are accepted for
pharmaceuticals and
nutritional supplements and physiologically unobjectionable, for example: as
fillers cellulose
derivatives (e.g. microcrystalline cellulose), sugars (e.g. lactose), sugar
alcohols (e.g. mannitol,
sorbitol), inorganic fillers (e.g. calcium phosphates), binders (e.g.
polyvinylpyrrolidone, gelatin,
starch derivatives and cellulose derivatives), and all other excipients
required to produce
formulations of pharmaceuticals and nutritional supplements of the desired
properties, e.g.
lubricants (magnesium stearate), e.g. disintegrants (e.g. crosslinked
polyvinylpyrrolidone, sodium
carboxymethylcellulose), e.g. wetting agents (e.g. sodium lauryl sulphate),
e.g. release-slowing
agents (e.g. cellulose derivatives, polyacrylic acid derivatives), e.g.
coloured pigments.
Excipients for pharmaceuticals and nutritional supplements familiar to the
skilled person are also
described for example in the following handbook: "Handbook of Pharmaceutical
Excipients",
Rowe R.C., Sheskey P.J. & Weller, P.J., American Pharmaceutical Association,
Washington, 4th
edition 2003.
The orodispersible tablet mentioned herein is produced by general standard
processes. The solid
dispersion is produced as described in EP 1 523 974. E.g. tablets can be
produced by mixing
and/or granulating the active ingredients together with the excipients (e.g.
the whole solid
dispersion part) to form a blend which is finally pressed to tablets.
Optionally different blends
containing different ingredients and excipients can be premixed and combined
to a final blend
which is then pressed to tablets.
Advantage of the composition of the present invention is that for the
preparation of the
composition a complex and expensive taste masking technology known in the
prior art such as
coating of tablets or granules, adding of antioxidants, or putting the PUFA
into a capsule is not
needed. The composition of the present invention can be prepared by simple and
well-known
standard procedures. Another well-known taste masking method is the addition
of flavours in order
to cover and mask the bad smell. This taste masking method is normally
restricted to only a few
applicable flavours which have to be selected in each case. However,
flavouring ingredients are
not needed for taste masking in the composition of the present invention.
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Preference is given to an orodispersible composition which is not a coated
tablet, coated granule,
or capsule or which does not comprise an antioxidant or flavour or other taste
masking substance.
Preference is given to a fast disintegrating orodispersible tablet, that means
it disintegrates rapidly
in the oral cavity. The disintegration time of the fast disintegrating
orodispersible tablet is less
equal than 100 sec., preferably less equal than 80 sec.
The composition according to the present invention shows a good and/or fast
resorption of the
PUFA after administration of the composition. Furthermore the composition
facilitates a quick
intake, optionally without water or drink.
The composition according to the invention shows an acceptable hardness and
friability to be
manufactured without affecting the beadlet integrity, i.e. no PUFA exudates
from the tablet matrix.
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Examples:
Example 1:
An orodispersible tablet consisting of:
= 500 mg of dry fish oil powder (omega-3, 18:12) including 23.5 mg of EPA
and 16.5 mg of
DHA
= 500 mg of F-melt type C
and optionally the following excipients:
= 15 mg of anhydrous citric acid
= 10 mg of Aspartam
F-melt type C is a commercially available disintegrant and consists of
crospovidone, mannitol,
xylitol, dibasic calcium phosphate and rnicrocrystalline cellulose (Fuji,
Chemical Industry Co.,
Ltd., Japan) and can be prepared as described in EP 1 523 974.
Example 2:
An orodispersible tablet consisting of:
= 890 mg of dry fish oil powder (omega-3, 18:12) including 41.83 mg of EPA
and 29.37 mg
of DHA
= 750 mg of F-melt type C
and optionally the following flavours and excipients:
= 25 mg of anhydrous citric acid
= 35 mg of Aspartam
= 5 mg of stearic acid
= 5 mg of iron oxide (red)
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F-melt type C is a commercially available disintegrant and consists of
crospovidone, mannitol,
xylitol, dibasic calcium phosphate and microcrystalline cellulose (Fuji,
Chemical Industry Co.,
Ltd., Japan) and can be prepared as described in EP 1 523 974.
Example 3:
An orodispersible tablet consisting of:
= 500 mg of dry fish oil powder (omega-3, 18:12) including 23.5 mg of EPA
and 16.5 mg of
DHA
= 500 mg of F-melt type C
and optionally the following flavours and excipients:
= 15 mg of anhydrous citric acid
= 50 mg of Lemon flavour
= 20 mg of honey flavour
= 10 mg of Aspartam
F-melt type C is a commercially available disintegrant and consists of
crospovidone, mannitol,
xylitol, dibasic calcium phosphate and microcrystalline cellulose (Fuji,
Chemical Industry Co.,
Ltd., Japan) and can be prepared as described in EP 1 523 974.
Example 4:
An orodispersible tablet consisting of:
= 890 mg of dry fish oil powder (omega-3, 18:12) including 41.83 mg of EPA
and 29.37 mg
of DHA
= 750 mg of F-melt type C
and optionally the following flavours and excipients:
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= 25 mg of anhydrous citric acid
= 50 mg of Lemon flavour
= 20 mg of honey flavour
= 30 mg of pineapple flavour
= 35 mg of Aspartam
= 5 mg of stearic acid
= 5 mg of iron oxide (red)
F-melt type C is a commercially available disintegrant and consists of
crospovidone, mannitol,
xylitol, dibasic calcium phosphate and naicrocrystalline cellulose (Fuji,
Chemical Industry Co.,
Ltd., Japan) and can be prepared as described in EP 1 523 974.
Manufacturing process for orodispersible tablets
Direct compression process :
All ingredients are mixed together in a tumble mixer for 20 min. and
optionally 5 min. before the
end the lubricant, if any, (stearic acid) is added. The final blend is pressed
into tablets with a rotary
press.
Results:
Examples 1 and 2 do not show any bad or fishy taste or smell, in examples 3
and 4 only small
amounts of flavour are added to improve the taste of the otherwise savourless
tablet.
The disintegration time is 40 5 sec. for example 1 and 3 and 60 5 sec. for
example 2 and 4.
