Note : Les descriptions sont présentées dans la langue officielle dans laquelle elles ont été soumises.
BIOASSEMBLY METHOD FOR SYNTHESIS USING MULTI WAVELENGTH FARADAY
WAVES AND USE THEREOF
TECHNICAL FIELD
The present disclosure relates to the technical field of tissue engineering
and biological
manufacturing under biomedical engineering, and particularly relates to a
biological assembly
method and use for a Faraday wave multi-wavelength synthesis.
BACKGROUND
In human tissues and organs, cells are interconnected through self-
organization to foirn a highly
ordered cell structure. The arrangement mode of cells in an in-vitro reduction
structure and the
geometrical shape of structure not only play a key role in simulating
morphologies of in-vivo tissues
and organs at a macroscopic scale, but also play an important role in
regulating the microenvironment
of cells.
In recent years, biological manufacturing has shown great potential in
constructing cellular
microstructures that highly restore the complexity and functionality of human
tissues and organs.
Biological manufacturing is to construct living cells, biological materials
and stimulating factors into
a biological product with specific structures and functions using biological
printing or biological
assembly, as well as subsequent tissue maturation processes. Among them,
biological assembly is
considered an important construction strategy in biological manufacturing,
with the advantage that
compared to biological printing, biological assembly can have better bio-
compatibility and higher
efficiency in constructing cell structures. So far, a series of biological
assembly technologies are
utilizing the interaction between external physical fields and cells to
manipulate the arrangement of
cells in space. Such physical fields comprise gravity fields, magnetic fields,
and electric fields, etc.
However, the cell structures constructed by biological assembly techniques
based on the above
principles often only have simple geometric shapes such as spherical, bar
shaped, and circular, which
are difficult to meet the needs of constructing multi-scale, complex and
arbitrary cell structures.
Compared with other biological assembly technologies, acoustic biological
assembly has the
unique advantage of a dynamically adjustable sound field, so that it can
provide better cell structure
diversity. Among them, acoustic holography technology has been used to
construct complex and
arbitrary cell structures. However, this technology has the problems of being
a complex system,
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arbitrary cell structures. However, this technology has the problems of being
a complex system,
complicated operation process, and incapability of adjusting the complex cell
structure in real time.
Compared to this technology, Faraday wave biological assembly technology has
advantages such as
easy system construction, simple operation, dynamically adjustable patterns,
and good
biocompatibility, etc. and has broad application prospects in the field of
biological tissue engineering.
However, the existing Faraday wave biological assembly technology only
performs single-
wavelength cell assembly in its frequency domain, and a limited cell structure
in a single scale capable
of being constructed fails to meet the requirements of constructing the multi-
scale, complex and
arbitrary cell structures.
Therefore, providing a biological assembly method that can meet the needs of
achieving the
complex and arbitrary arrangement of multi-scale cells in biological tissue
engineering research has
important application prospects and also technical promotion and application
value.
SUMMARY
The problem to be solved by the present disclosure is to provide a biological
assembly method
for multi-wavelength synthesis of a Faraday wave and an application thereof
aiming at shortcomings
in an existing biological assembly technology. The present disclosure is
intended to excite and form
the Faraday wave obtained by multi-wavelength synthesis by synthesizing a
plurality of sine or cosine
signals with different wavelengths, a single-wavelength assembly mode in a
traditional Faraday wave
frequency domain is expanded into a multi-wavelength assembly mode, and
complex and arbitrary
pattern arrangement of liquid-bottom multi-scale cells is realized, so that
the biological assembly of
the Faraday wave has higher technical popularization and application values.
In order to achieve the above objective, a working principle of the present
disclosure is as
follows:
An acoustic biological assembly theory of multi-wavelength synthesis is
established. In an
acoustic field of Faraday waves, the liquid-bottom cells are subjected to the
combined actions of
acoustic pressure, buoyancy and gravity, wherein the acoustic pressure plays a
decisive role in a cell's
equilibrium position, and the cells are finally equilibrated to a position
with the lowest potential
energy in an acoustic pressure field, which is namely a potential well
position. Any complex acoustic
pressure fields may be simplified into superposition of a series of sine or
cosine acoustic pressure
fields by a Fourier series. Therefore, a plurality of sine or cosine signals
with different wavelengths
may be synthesized into a complex waveform, so as to meet the requirements of
complex and arbitrary
arrangement of multi-scale cells. A multi-scale complex structure of multi-
wavelength synthesis is
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shown in FIG. I. For example, the liver is an important organ with a unique
multi-scale structure,
with an adult liver being composed of 0.5 to 1 million hexagonal hepatic
lobules. A periodic hepatic
lobule structural unit, and a multi-scale micro-physiological structure of a
liver tissue are shown in
FIG. 2. Therefore, a complex liver tissue structure may be simplified into a
liver lobule structure
arranged repeatedly in space. Complex acoustic pressure field distribution is
realized in space by
multi-wavelength synthesis, thus realizing the construction of a multi-scale
liver cell structure. In
addition, there are a large number of tissues and organs in the human body
that contain specific multi-
scale structural units, for example, pulmonary alveoli are mainly
hemispherical vesicles composed of
monolayer epithelial cells; and renal tubules are mainly hollow tubular
structures formed by self-
organization of renal tubular epithelial cells. Physiological structures of
these specific tissues and
organs may all be constructed in vitro by assembly for the multi-wavelength
synthesis of the Faraday
wave.
In order to achieve the above objective, a technical solution of the present
disclosure is as
follows:
In a first aspect, the present disclosure provides a cell assembly method for
multi-wavelength
synthesis of Faraday waves, wherein any complex periodic pattern is capable of
being simplified into
superposition of a series of sine or cosine waves by a Fourier series, with a
plurality of sine or cosine
signals with different wavelengths being synthesized in the method, thus
exciting and forming the
Faraday wave obtained by multi-wavelength synthesis, and finally, complex and
arbitrary
arrangement of multi-scale cells is realized; while the node is a node
position of a Faraday standing
wave, the anti-node is an anti-node position of the Faraday standing wave, and
the method comprises
the following steps:
Si: Synthesizing the sine or cosine signals with different wavelengths in a
waveform creating
and editing tool software, according to a biological assembly pattern to be
constructed, and inputting
a synthesized multi-wavelength signal file into an arbitrary waveform/function
signal generator;
S2: Opening the multi-wavelength signal file synthesized in Si in the
arbitrary
wavefoini/function signal generator, outputting a corresponding electric
signal through the arbitrary
waveform/function signal generator, transferring the electric signal to a
power amplifier for power
amplification, transferring the amplified fidelity electric signal to a
vibration exciter to generate stable
and periodic vibration, and connecting an assembly chamber with the vibration
exciter for horizontal
calibration;
S3: Evenly adding a suspension of cell-containing assembly units to be
assembled into the
assembly chamber, and carrying out assembly for the multi-wavelength synthesis
of the Faraday wave
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after the cell-containing assembly unit sediments to a bottom portion of the
assembly chamber.
Specific steps are as follows:
In Sl, according to a cell pattern arranged by target assembly, the synthesis
of Faraday waves
with different wavelengths is realized in the waveform creating and editing
tool software, and the
frequency is selected in range from 1 Hz to 1,000 Hz. For example, for
synthesis of a dual-wavelength
Faraday wave, a written sine signal function is y = A1 * sin(fi * 2 * it * x)
+ A2 * sin(f2 * 2 * it *
x); and a written cosine signal function is y = A1 * cos(fi * 2 *7r * x) + A2
* COS(f2 * 2 * it * x).
fi is a low driving frequency, f2 is a high driving frequency, and the ratio
of a corresponding
amplitude A1 of the sine or cosine signal function of the low driving
frequency to a corresponding
amplitude A2 of the sine or cosine signal function of the high driving
frequency ranges from 1: 1 to
1: 5. For synthesis of a multi-wavelength Faraday wave, a written sine signal
function is y = A1 *
sin(fi * 2 * 7 * X) + A2 * sin(f2 * 2 * 7r * x) + A3 * sin(f3 * 2 * ir * x) +
A4 * sin(f4 * 2 * 7r *
x) + A5 * sin(f5 * 2 * 7r * x) + = = .; and a written cosine signal function
is y = Ai * cos(fi * 2 * 7 *
x) + A2 * COS(f2 * 2 * 7r * x) + A3 * COS(f3 * 2 * 7r * x) + A4 * COS(f4 * 2 *
7r * x) + A5 * COS(f5 *
2 * it * x) + .... The frequencies satisfy that f1 <f2 <f3 <f4 <f5; the
amplitudes satisfy that
A1 A2 A3 A4 A5, and a ratio of a lower amplitude to a higher
amplitude ranges from 1: 1
to 1: 5. The synthesized multi-wavelength signal file is input into the
arbitrary waveform/function
signal generator.
In S2, the synthesized multi-wavelength signal file is opened, the specific
electric signal is
output through the arbitrary waveform/function signal generator, the electric
signal is transferred to
the power amplifier for power amplification, and the amplified fidelity
electric signal is transferred
to the vibration exciter to generate stable and periodic vibration. The
assembly chamber is connected
with the vibration exciter for horizontal calibration. A shape of the assembly
chamber may be a circle,
a square, a rectangle, a triangle, a trapezoid, a diamond, a hexagon and an
octagon; and the assembly
chamber in each shape has a circumscribed circle diameter of 0.5 cm to 20 cm;
and a height of 0.2
mm to 10 mm.
In S3, in the cell-containing assembly unit used for assembly, a selected cell
is one or a mixture
of several of either an embryonic stem cell, an induced pluripotent stem cell,
a cancer stem cell, or a
mesenchymal stem cell; or the selected cell is any one of the above or a
mixture of several primary
cells, progenitor cells, precursor cells, or diseased cells of tissues and
organs comprising brain, liver,
kidney, pancreas, blood vessel, heart, skin, bone marrow, bone, cartilage and
muscle. The cell-
containing assembly unit is one or a mixture of several of either a single
cell, a micro-tissue block,
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an organoid, a cell microsphere, a cell-containing hydrogel microsphere,
and/or a cell-containing
carrier particle, with a diameter of 2 pm to 5,000 lim. The number of the cell-
containing assembly
units range from 2 to 1012. The cell-containing assembly units are evenly
dispersed in a phosphate
buffered solution, a cell culture medium, a natural hydrogel, a synthetic
hydrogel, or a mixed hydrogel
for later use, with a buoyant density of 1 g/cm3 to 10 g/cm3. The suspension
of the cell-containing
assembly unit to be assembled is evenly added into the assembly chamber, and
assembly for the multi-
wavelength synthesis of the Faraday wave is carried out after the cell-
containing assembly unit
sediments to the bottom portion of the assembly chamber.
In a second aspect, the present disclosure provides an application of the cell
assembly method
for the multi-wavelength synthesis of the Faraday wave above, wherein the
method is applied to
construction of artificial tissues and organs by using a biological assembly
unit containing living cells,
and the constructed artificial tissues and organs are capable of being used in
cellular artificial meat, a
drug testing model, and a clinical tissue and organ repair product.
The present disclosure has the advantages and the generated beneficial effects
as follows:
The cell assembly method for the multi-wavelength synthesis of the Faraday
waves according
to the present disclosure has the advantages of easy establishment of a
system, simple manipulation,
a dynamically adjustable pattern, good biocompatibility, and the like. The
present disclosure is
different from other cell manipulation techniques in principle under a single-
wavelength condition in
an existing acoustic biological assembly method, in which sine or cosine
signals with different
wavelengths are synthesized, thus exciting and forming the Faraday wave
obtained by multi-
wavelength synthesis, a single-wavelength assembly mode in a traditional
Faraday wave frequency
domain is expanded into a multi-wavelength assembly mode, and complex and
arbitrary pattern
arrangements of liquid-bottom multi-scale cells is realized, so that the
present disclosure is more
suitable for the requirements of complex arrangement of cells in tissue
engineering and biological
manufacturing, and has huge application prospects and commercial conversion
potential.
DESCRIPTION OF THE DRAWINGS
FIG. 1 is a schematic diagram of a multi-scale complex structure of multi-
wavelength synthesis.
FIG. 2 is a schematic diagram of the periodic structural units and multi-scale
micro-
physiological structures of liver tissue.
FIG. 3 is a schematic diagram of single-wavelength, dual-wavelength and three-
wavelength
polystyrene microsphere patterns obtained by liquid-bottom assembly in Example
1 of the present
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disclosure.
FIG. 4 is a schematic diagram of the single-wavelength and dual-wavelength
HepG2 cell
microsphere patterns obtained by liquid-bottom assembly in Example 2 of the
present disclosure.
DETAILED DESCRIPTION
The following will explain the technical solution of the present disclosure in
conjunction with
examples. Those skilled in the art will understand that the following examples
are only intended
toillustrate the present disclosure and should not be considered to limit the
scope of the present
disclosure. Specific technologies or conditions not specified in the examples
should follow the
technologies or conditions described in the literature in the art or be
carried out in accordance with
the product specification.
Example 1: Assembly of Faraday wave multi-wavelength synthesis for the
arrangement of
polystyrene microspheres
Polystyrene microspheres were added into 0.01 M phosphate buffer solution
(PBS) containing
0.05% Tween 20, and fully dispersed. The polystyrene microspheres were red in
color, and had a
diameter of 100 lam, a density of 1.05 g/cm3, and physical properties similar
to that of cell-containing
assembly units, and were at a concentration of 30 mg,/mL. In this example, the
polystyrene
microspheres were used to simulate the cell-containing assembly units.
1. The arrangement of polystyrene microspheres under single-wavelength
conditions, steps are
as follows:
After setting the waveform as a continuous sine wave, the driving frequencies
were set to be 20
Hz, 28 Hz and 58 Hz, and the signal amplitude was 60 mVpp to 100 mVpp. A
specific electric signal
was output by adjusting any waveform/function signal generator (Tecictronix,
AFG3052C), the
electric signal was transferred to a power amplifier (DAYTONAUDIO, DTA-120)
through a BNC
bus-to-RCA common line for power amplification. The amplified fidelity
electric signal was
transferred to a vibration exciter (Wuxi Shiao Technology Co., Ltd., SA-
JZOO5T) through an RCA
common converted four-core socket to generate stable and periodic vibrations.
After the circular
assembly chamber (0=3 cm) was rigidly connected to the vibration exciter
through M5x30, 360
horizontal calibration was carried out by a circular spirit level. Finally,
the suspension of polystyrene
microspheres to be assembled was evenly added into the circular assembly
chamber, and Faraday
wave assembly was carried out after the polystyrene microspheres settled to
the bottom of the
assembly chamber. The pattern obtained by assembling polystyrene microspheres
under single
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wavelength conditions was shown in FIG. 3 (a), which can only form relatively
simple divergent,
petal-shaped and concentric circular patterns.
2. The arrangement of polystyrene microspheres under dual-wavelength synthesis
condition,
steps are as follows:
The synthesis of two sine signals with different wavelengths was realized by
using "ArbExpress"
software. The wave function of the synthesized sine signal synthesized at the
corresponding
wavelengths of driving frequencies 20 Hz and 28 Hz was y = 1.5 x sin(20 x 2 x
it x x) +
sin(28 x 2 x it x x) . The wave function of the synthesized sine signal at the
corresponding
wavelengths of driving frequencies 20 Hz and 58 Hz was y = 1.2 x sin(20 x 2 x
it x x)+
sin(58 x 2 x it x x) . The wave function of the synthesized sine signal at the
corresponding
wavelengths of driving frequencies 28 Hz and 28 Hz was y = sin(20 x 2 x it x
x) + sin(28 x 2 x
it x x). The synthesized dual-wavelength sine signal was saved as a "TFW"
format file, and imported
into a waveform/function signal generator (Tecktronix, AFG3052C).
After setting the waveform to the desired waveform, the synthesized dual-
wavelength sine signal
files were opened in sequence, and the signal amplitude was 140 mVpp to 160
mVpp. By outputting
a specific electric signal through any waveform/function signal generator, the
electric signal was
transferred to the power amplifier (DAYTONAUDIO, DTA-120) through a BNC bus-to-
RCA
common linefor power amplification. The amplified fidelity electric signal was
transferred to a
vibration exciter (Wuxi Shiao Technology Co., Ltd., SA-JZOO5T) through an RCA
common
converted four-core socket to generate stable and periodic vibration. After
the circular assembly
chamber (0=3 cm) was rigidly connected to the vibration exciter through M5
x30, 360 horizontal
calibration was carried out by a circular spirit level. Finally, the
suspension of polystyrene
microspheres to be assembled was evenly added into the circular assembly
chamber, and Faraday
wave assembly was carried out after the polystyrene microspheres settled to
the bottom of the
assembly chamber. The pattern obtained by assembling polystyrene microspheres
under dual-
wavelength conditions is shown in FIG. 3 (b), which achieves the superposition
of corresponding
patterns under the single-wavelength conditions, resulting in a pattern of
divergent, petal-shaped and
concentric circular patterns overlapping each other.
3. The arrangement of polystyrene microspheres under three-wavelength
synthesis condition,
steps are as follows:
The synthesis of three sine signals with different wavelengths was realized by
using
"ArbExpress" software. The wave function of the synthesized sine signal at the
corresponding
wavelengths of driving frequencies 20 Hz, 28 Hz and 58 Hz was y = 1.4 x sin(20
x 2 x it x x) +
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sin(28 x 2 x it x x) + sin (58 x 2 x it x x) . The synthesized three-
wavelength sine signal was
saved as a "TFW" format file, and imported into a waveform/function signal
generator (Tecktronix,
AFG3052C).
After setting the waveform to the desired waveform, the synthesized three-
wavelength sine
signal file was opened, and the signal amplitude was 200 mVpp. By outputting a
specific electric
signal through any waveform/function signal generator, the electric signal was
transferred to the
power amplifier (DAYTONAUDIO, DTA-120) through a BNC bus-to-RCA common line
for power
amplification. The amplified fidelity electric signal was transferred to a
vibration exciter (Wuxi Shiao
Technology Co., Ltd., SA-JZOO5T) through an RCA common converted four-core
socket to generate
stable and periodic vibration. After the circular assembly chamber (4130=3cm)
was rigidly connected to
the vibration exciter through M5 x30, 360 horizontal calibration was carried
out by a circular spirit
level. Finally, the suspension of polystyrene microspheres to be assembled was
evenly added into the
circular assembly chamber, and Faraday wave assembly was carried out after the
polystyrene
microspheres settled to the bottom of the assembly chamber. The pattern
obtained by assembling
polystyrene microspheres under three-wavelength conditions was shown in FIG. 3
(c), which further
achieves the complex and arbitrary pattern arrangement of the polystyrene
microspheres under multi-
scale conditions, and the assembly pattern had characteristics of divergent,
petal-shaped and
concentric circular patterns all at the same time.
Example 2: Assembly of Faraday wave multi-wavelength synthesis for the
arrangement of
HepG2 cell microspheroids
The arrangement of the HepG2 cell microspheroids under single-wavelength
condition, steps as
follows:
HepG2 cells were added into a six-well low-adhesion plate (Corning, 3471),
with 2x 106 cells in
each well, and added with 2 mL of DMEM complete culture medium. The six-well
low-adhesion
plate was placed on a flat shaker at a rotational speed of 75 rpm, and
cultured for three days to form
cell spheroids. HepG2 cell microspheroids in one well were collected and added
with 0.01 M
phosphate buffer solution for later use.
After setting the waveform as a continuous sine wave, the driving frequency
was set to be 42 Hz
and 45 Hz, and the signal amplitude was 60 mVpp to 80 mVpp. A specific
electric signal was output
by adjusting a waveform/function signal generator (Tecktronix, AFG3052C), the
electric signal was
transferred to a power amplifier (DAYTONAUDIO, DTA-120) through a BNC bus-to-
RCA common
line for power amplification. The amplified fidelity electric signal was
transferred to a vibration
exciter (Wuxi Shiao Technology Co., Ltd., SA-JZOO5T) through an RCA common
converted four-
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core socket to generate stable and periodic vibration. After the black-
background circular assembly
chamber (43=2 cm) with black background was rigidly connected to the vibration
exciter through
M5 x30, 3600 horizontal calibration was carried out by a circular spirit
level. Finally, the suspension
of HepG2 cell microspheroids to be assembled was evenly added into the
circular assembly chamber,
and Faraday wave assembly was carried out after the HepG2 cell microspheroids
settled to the bottom
of the assembly chamber. The pattern obtained by assembling the HepG2 cell
microspheroids under
single wavelength conditions was shown in FIG. 4 (a), which can only form
relatively simple
divergent and four-leaf petal patterns.
The arrangement of the HepG2 cell microspheroids under dual-wavelength
synthesis condition,
steps are as follows:
The synthesis of two sine signals with different wavelengths was realized by
using "ArbExpress"
software. The wave function of the synthesized sine signal at the
corresponding wavelengths of
driving frequencies 42 Hz and 45 Hz was y = 2.1 x sin(42 x 2 x it x x) +
sin(45 x 2 x it x x).
The synthesized dual-wavelength sine signal was saved as a "TFW" format file,
and imported into a
waveform/function signal generator (Tecktronix, AFG3052C).
After setting the waveform as the desired waveform, the synthesized dual-
wavelength sine signal
file was opened, and the signal amplitude was 140 mVpp to 160 mVpp. The
assembly of the HepG2
cell microspheroids under the dual-wavelength condition was completed by the
aforementioned
Faraday wave acoustic biological assembly system. The pattern obtained by
assembling HepG2 cell
microspheroids under the dual-wavelength condition was shown in FIG. 4 (b),
which achieves the
superposition of corresponding patterns under the single-wavelength
conditions. Under the multi-
scale condition, HepG2 cell microspheroids are arranged in complex and
arbitrary patterns, with
assembly patterns had characteristics of divergent and four-leaf petal
patterns at the same time.
In summary, the present disclosure innovatively synthesizes a plurality of
sine or cosine
signals of different wavelengths to excite and form the Faraday waves for
multi-wavelength
synthesis, achieving complex and arbitrary cell arrangement patterns under the
multi-scale
condition. The system in this method is easy to construct, simple to operate,
dynamically
adjustable in pattern, and has better bio-compatibility. More importantly,
this method differs
from the cell manipulation principle of existing acoustic biological assembly
methods under
single-wavelength condition. By exciting the formation of Faraday waves for
multi-wavelength
synthesis, the single-wavelength assembly mode in the traditional Faraday wave
frequency
domain is expanded to a multi-wavelength assembly mode, achieving multi-scale,
complex and
an arbitrary pattern of cells arrangement at the liquid bottom. It provides an
innovative
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solution to the demand for multi-scale, complex and arbitrary cells
arrangement in tissue
engineering and biological manufacturing applications.
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