Note : Les descriptions sont présentées dans la langue officielle dans laquelle elles ont été soumises.
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The invention disclosed herein relates to the fields of genomics and molecular
biology. More specifically the invention relates to new high through-put
methods of
making libraries of expressed gene sequences and the libraries made using said
methods.
Recent breakthroughs in nucleic acid sequencing technology have made
possible the sequencing of entire genomes from a variety of organisms,
including
humans. The potential benefits of a complete genome sequence are many, ranging
from applications in medicine to a greater understanding of evolutionary
processes.
These benefits cannot be fully realized, however, without an understanding of
how
and where these newly sequenced genes function.
Traditionally, functional understanding started with recognizing an activity,
isolating a protein associated with that activity, then identifying and
isolating the
gene, or genes, encoding that protein. Each gene of interest was identified,
isolated
and expressed separately, a relatively time consuming process.
Recently, breakthroughs in high through-put DNA sequencing technology
have allowed massive amounts of gene sequence information to become available
to
the public. Yet methods of expressing these sequences to produce the proteins
encoded by them for study have still required that each sequence be
manipulated one
at a time. Accordingly, a need exists for the development of methods for the
rapid,
simultaneous expression of large numbers of gene sequences. The invention
described herein addresses this and related needs as will become apparent upon
inspection of the specification and the appended claims.
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Brief R~sc~'ntion of the Invention
The present invention comprises a method for producing libraries of
expressible gene sequences. The method of the invention allows for the
simultaneous
manipulation of multiple gene sequences and thus allows libraries to be
created in an
efficient and high through-put manner. The expression vectors containing
verified
gene sequences can be used to transfect cells for the production of
recombinant
proteins. The invention method utilizes known techniques in such a way as to
create
an efficient high through-put means of producing libraries of expressible gene
sequences.
The invention further comprises libraries of expressible gene sequences
produced using the method of the invention and expression vectors used in the
construction of such libraries.
Figure 1 shows a schematic representation of the vaccinia topoisomerase type
I cloning method used in the practice of the invention.
The present invention comprises a method for producing libraries of
expressible gene sequences. The invention method comprises the following
steps:
amplifying a plurality of gene sequences, purifying the amplified gene
sequences,
inserting each of the purified gene sequences into an expression vector, and
verifying
the size and orientation of the inserted gene sequence.
In the first step, the gene sequences that are to be expressed are amplified.
By
"amplification" it is meant that the copy number of the gene sequences) is
increased.
One commonly used method of amplification is the polymerase chain reaction
(PCR).
In brief, starter DNA is heat-denatured into single strands. Two synthetic
oligonucleotides, one complementary to sequence at the 3' end of the sense
strand of
DNA segment of interest and the other complementary to the sequence at the 3'
end of
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the anti-sense strand of a DNA segment of interest, are added in great excess
to the
DNA sequence to be amplified and the temperature is lowered to 50 - 60°
C. The
specific oligonucleotides hybridize with the complementary sequences in the
DNA
and then serve as primers of DNA chain synthesis, which requires the addition
of a
supply of deoxynucleotides and a temperature-resistant DNA polymerase, such as
Taq
polymerase, which can extend the primers at temperatures up to 72° C.
When
synthesis is complete, the whole mixture is heated further (up to 95°
C) to melt the
newly formed DNA duplexes. When the temperature is lowered again, another
round
of synthesis takes place, since an excess of primer is still present. Repeated
cycles of
synthesis and melting quickly amplify the sequence of interest. A more
detailed
description of PCR can be found in Erlich, Ed, PCR Technology: Principles and
Applications for DNA Amplification, W.H. Freeman and Co., 1992 and Erlich, et
al,
Eds., Polymerase Chain Reaction, Cold Spring Harbor Laboratory, 1989, both of
which are incorporated by reference herein.
Starter DNA can come from a variety of sources. It can be total genomic
DNA from an organism, for example, or can be cDNA that has been synthesized
from
cellular mRNA using reverse transcriptase. Sources of suitable RNA include
normal
and diseased tissues, cellular extracts, and the like.
In practicing the method of the invention, the desired gene sequences can
come from any source. The examples presented below show the amplification of
all
open reading frames (ORFs) from a single organism, Saccharomyces cerevisiae,
for
example. By "open reading frame" it is meant a segment of DNA that exists
between
a start codon and a stop codon and is likely to represent a gene. The examples
presented below further show the amplification of a group of human genes
thought to
be important in the development of cancer.
Public databases exist that contain the entire or partial genome of a
particular
organism, for example yeast (Saccharomyces cerevisiae), prokaryotes (Bacillus
subtilis, E. coli, Borrelia burgdorferi, Helicobacter pylori, Mycoplasma
genitalium,
and the like), fish (Fugu rubripes), mammals (human, mouse), plants (rice,
cotton)
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and the like. Well known databases include GenBank, Unigene, EMBL, IMAGE and
TIGR, for example. Public databases such as these can be used a source of gene
sequences for use in the method of the invention.
The primers employed in the amplification step are specific for each desired
gene sequence and include a variety of unique features. For example, the 5'
"sense"
primer starts with the sequence S'-CACC~... (the start codon is underlined).
The
CACC sequence is added as a Kozak consensus that aids in translational
efficiency.
When the gene sequence being amplified represents a full-length gene, the 3'
"antisense" codon is preferably designed to make the amplification product end
at the
3rd position of the last codon of the gene being amplified, plus a single
adenine
residue. This facilitates the fusion of the coding region in-frame with a
heterologous
peptide sequence such as an epitope tag, an affinity purification tag, and the
like (see
below). The gene sequence need not encode a full-length sequence, however, as
the
invention methods are equally suitable for any gene sequence, including
Expressed
Sequence Tags (ESTs). The primers can be synthesized and dried in multiwell
formats, such as 96-well microtiter plates to facilitate identification and
fiuther
processing.
The amplified gene products are next isolated from the other components of
the amplification reaction mixture. This purification can be accomplished
using a
variety of methodologies such as column chromatography, gel electrophoresis,
and the
like. A preferred method of purification utilizes low-melt agarose gel
electrophoresis.
The reaction mixture is separated and visualized by suitable means, e.g. by
ethidium
bromide staining. DNA bands that represent correctly sized amplification
products
are cut away from the rest of the gel and placed into appropriate
corresponding wells
of a 96-well microtiter plate. These plugs are subsequently melted and the DNA
contained therein utilized as cloning inserts. The use of gel electrophoresis
has the
advantage that the practitioner can purify the desired amplified gene sequence
while
additionally verifying that the sequence is of the correct size, i.e.,
represents the entire
desired gene sequence.
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The purified, amplified gene sequences are next inserted into an expression
vector. A variety of expression vectors are suitable for use in the method of
the
invention, both for prokaryotic expression and eukaryotic expression. In
general, the
expression vector will have one or more of the following features: a promoter-
enhancer sequence, a selection marker sequence, an origin of replication, an
affinity
purification tag sequence, an inducible element sequence, an epitope-tag
sequence,
and the like.
Promoter-enhancer sequences are DNA sequences to which RNA polymerase
binds and initiates transcription. The promoter determines the polarity of the
transcript by specifying which strand will be transcribed. Bacterial prnmoters
consist
of consensus sequences, -35 and -10 nucleotides relative to the
transcriptional start,
which are bound by a specific sigma factor and RNA polymerase. Eukaryotic
promoters are more complex. Most promoters utilized in expression vectors are
transcribed by RNA polymerase II. General transcription factors (GTFs) first
bind
1 S specific sequences near the start and then recruit the binding of RNA
polymerase II.
In addition to these minimal promoter elements, small sequence elements are
recognized specifically by modular DNA-binding/trans-activating proteins (e.g.
AP-1,
SP-1) which regulate the activity of a given promoter. Viral promoters serve
the same
function as bacterial or eukaryotic promoters and either provide a specific
RNA
polymerase in traps (bacteriophage T7) or recruit cellular factors and RNA
polymerase (SV40, RSV, CMV). Viral promoters are preferred as they are
generally
particularly strong promoters.
Promoters may be, furthermore, either constitutive or, more preferably,
regulatable (i.e., inducible or derepressible). Inducible elements are DNA
sequence
elements which act in conjunction with promoters and bind either repressors
(e.g.
lac0/LAC Iq repressor system in E. coli) or inducers (e.g. gall/GAL4 inducer
system
in yeast). In either case, transcription is virtually "shut off' until the
promoter is
derepressed or induced, at which point transcription is "turned-on".
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Examples of constitutive promoters include the int promoter of bacteriophage
~,, the bla promoter of the ø-lactamase gene sequence of pBR322, the CAT
promoter
of the chloramphenicol acetyl transferase gene sequence of pPR325, and the
like.
Examples of inducible prokaryotic promoters include the major right and left
promoters of bacteriophage (PL and P~, the trp, reca, lacZ, LacI, Ara.C and
gal
promoters of E. coli, the a.-amylase (Ulmanen et al., J. Bacteriol. x:176-182,
1985)
and the sigma-28-specific promoters of B. subtilis (Gilman et al., Gene
sequence
32:11-20(1984)), the promoters of the bacteriophages of Bacillus (Gryczan, In:
The
Molecular Biology of the Bacilli, Academic Press, Inc., NY (1982)),
Streptomyces
promoters (Ward et al., Mol. Gen. Genet. x:468-478, 1986), and the like.
Exemplary prokaryotic promoters are reviewed by Glick {J. Ind. Microbiol.
x:277-
282, 1987); Cenatiempo (Biochimie x$:505-516, 1986); and Gottesman (Ann. Rev.
Genet.1$:415-442, 1984).
Preferred eukaryotic promoters include, for example, the promoter of the
mouse metallothionein I gene sequence (Hamer et al., J. Mol. Appl. Gen.1:273-
288,
1982); the TK promoter of Herpes virus (McKnight, Cell x:355-365, 1982); the
SV40 early promoter (Benoist et al., Nature (London) ~QQ:304-310, 1981); the
yeast
gal l gene sequence promoter (Johnston et al., Proc. Natl. Acad Sci. (USAI
22:6971-6975, 1982); Silver et al., Proc. Natl. Acad. Sci. (LISA) $1:5951-
5955,
1984), the CMV promoter, the EF-1 promoter, Ecdysone-responsive promoter(s),
and
the like.
Selection marker sequences are valuable elements in expression vectors as
they provide a means to select for growth only those cells which contain a
vector.
Such markers are of two types: drug resistance and auxotrophic. A drug
resistance
marker enables cells to detoxify an exogenously added drug that would
otherwise kill
the cell. Auxotrophic markers allow cells to synthesize an essential component
(usually an amino acid) while grown in media which lacks that essential
component.
Common selectable marker gene sequences include those for resistance to
antibiotics such as ampicillin, tetracycline, kanamycin, streptomycin,
bleomycin,
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hygromycin, neomycin, ZeocinTM, and the like. Selectable auxotrophic gene
sequences include, for example, hisD, which allows growth in histidine free
media in
the presence of histidinol.
A preferred selectable marker sequence for use in yeast expression systems is
URA3. Laboratory yeast strains carrying mutations in the gene which encodes
orotidine-5'-phosphate decarboxylase, an enzyme essential for uracil
biosynthesis, are
unable to grow in the absence of exogenous uracil. A copy of the wild-type
gene
(ura4+ in S. pombe and URA3 in S. cerevisiae) will complement this defect in
trans.
A further element useful in an expression vector is an origin of replication
sequence. Replication origins are unique DNA segments that contain multiple
short
repeated sequences that are recognized by multimeric origin-binding proteins
and
which play a key role in assembling DNA replication enzymes at the origin
site.
Suitable origins of replication for use in expression vectors employed herein
include
E coli oriC, 2p, and ARS (both useful in yeast systems), sfl, SV40 (useful in
I S mammalian systems), and the like.
Additional elements that can be included in expression vectors employed in
the invention method are sequences encoding affinity purification tags or
epitope tags.
Affinity purification tags are generally peptide sequences that can interact
with a
binding partner immobilized on a solid support. Synthetic DNA sequences
encoding
multiple consecutive single amino acids, such as histidine, when fused to the
expressed protein, may be used for one-step purification of the recombinant
protein by
high affinity binding to a resin column, such as nickel sepharose. An
endopeptidase
recognition sequence is often engineered between the polyamino acid tag and
the
protein of interest to allow subsequent removal of the leader peptide by
digestion with
a specific protease. Sequences encoding peptides such as the chitin binding
domain
(which binds to chitin), glutathione-S-transferase (which binds to
glutathione), biotin
(which binds to avidin or strepavidin), and the like can also be used for
facilitating
purification of the protein of interest. The affinity purification tag can be
separated
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from the protein of interest by methods well known in the art, including the
use of
inteins (protein self splicing elements, Chong, et al, Gene .122:271-281,
1997).
Epitope tags are short peptide sequences that are recognized by epitope
specific antibodies. A fusion protein comprising a recombinant protein and an
epitope
tag can be simply and easily purified using an antibody bound to a
chromatography
resin. The presence of the epitope tag furthermore allows the recombinant
protein to
be detected in subsequent assays, such as Western blots, without having to
produce an
antibody specific for the recombinant protein itself. Examples of commonly
used
epitope tags include V5, glutathione-S-transferase (GST), hemaglutinin (HA),
the
peptide Phe-His-His-Thr-Thr, chitin binding domain, and the like.
A further useful element in an expression vector is a multiple cloning site or
polylinker. Synthetic DNA encoding a series of restriction endonuclease
recognition
sites is inserted into a plasmid vector downstream of the promoter element.
These
sites are engineered for convenient cloning of DNA into the vector at a
specific
1 S position.
The foregoing elements can be combined to produce expression vectors useful
in the practice of the present invention. Suitable prokaryotic vectors include
plasmids
such as those capable of replication in E. coli (for example, pBR322, ColEl,
pSC101,
PACYC 184, itVX, pRSET, pBAD (Invitrogen, Carlsbad, CA) and the like). Such
plasmids are disclosed by Sambrook (cf. "Molecular Cloning: A Laboratory
Manual", second edition, edited by Sambrook, Fritsch, & Maniatis, Cold Spring
Harbor Laboratory, (1989)). Bacillus plasmids include pC194, pC221, pT127, and
the
like, and are disclosed by Gryczan (In: The Molecular Biology of the Bacilli,
Academic Press, NY (1982), pp. 307-329). Suitable Streptomyces plasmids
include
p1J101 (Kendall et al., J. Bacteriol. xø2:4177-4183,1987), and streptomyces
bacteriophages such as ~C31 (Chater et al., In: Sixth International Symposium
on
Actinomycetales Biology, Akademiai Kaido, Budapest, Hungary (1986), pp. 45-
54).
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Pseudomonas plasmids are reviewed by John et al. (Rev. Infect. Dis. $:693-704,
1986), and Izaki (Jpn. J. Bacteriol. 3:729-742, 1978).
Suitable eukaryotic plasmids include, for example, BPV, vaccinia, SV40, 2-
micron circle, pcDNA3.1, pCDNA3.l/GS, pYES2/GS, pMT, p IND, pIND(Spl),
pVgRXR (Invitrogen), and the like, or their derivatives. Such plasmids are
well
known in the art (Botstein et al., Miami Wntr. Symp. ,x:265-274, 1982);
Broach, In:
"The Molecular Biology of the Yeast Saccharomyces: Life Cycle and
Inheritance",
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p. 445-470, 1981;
Broach,
Cell x$:203-204, 1982; Dilon et al., J Clin. Hematol. Oncol. I,Q:39-48, 1980;
Maniatis, In: Cell Biology: A Comprehensive Treatise, Vol. 3, Gene Sequence
Expression, Academic Press, NY, pp. 563-608, 1980).
Construction of chimaeric DNA molecules in vitro relies traditionally on two
enzymatic steps catalyzed by separate protein components. PCR amplification or
site-
specific restriction endonucleases are used to generate linear DNAs with
defined
termini that can then be joined covalently at their ends via the action of DNA
ligase.
DNA ligase has limitations, however, in that it is relatively slow acting and
temperature sensitive.
Thus, when inserting the purified, amplified gene sequence into the expression
vector the use of an enzyme that can both cleave and religate DNA in a site
specific
manner is preferred. Any site-specific enzyme of this type is suitable, for
example, a
type I topoisomerase or a site-specific recombinase. Examples of suitable site-
specific recombinases include lambda integrase, FLP recombinase, P1-Cre
protein,
Kw recombinase, and the like (Pan, et al, J. Biol. Chem. ?x$:3683-3689, 1993;
Nunes-Duby, et al, EMBO J.1~:4421-4430, 1994; Hallet and Sherratt, FEMS
Microbio. Revs ?,1:157-178, 1997; Ringrose, et al, Eur J. Biochem X4$:903-912,
1997).
A particularly suitable enzyme for use in the invention method is a type I
topoisomerase, particularly vaccinia DNA topoisomerase. Vaccinia DNA
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topoisomerase binds to duplex DNA and cleaves the phosphodiester backbone of
one
strand. The enzyme exhibits a high level of sequence specificity, akin to that
of a
restriction endonuclease. Cleavage occurs at a consensus pentapyrimidine
element
5'-(C/T)CCTT in the scissile strand. In the cleavage reaction, bond energy is
conserved via the formation of a covalent adduct between the 3' phosphate of
the
incised strand and a tyrosyl residue of the protein. Vaccinia topoisomerase
can
religate the covalently held strand across the same bond originally cleaved
(as occurs
during DNA relaxation) or it can religate to a heterologous acceptor DNA and
thereby
create a recombinant molecule.
10 When the substrate is configured such that the scissile bond is situated
near
(within 10 basepairs ofj the 3' end of a DNA duplex, cleavage is accompanied
by the
spontaneous dissociation of the downstream portion of the cleaved strand. The
resulting topoisomerase-DNA complex, containing a 5' single-stranded tail, can
religate to an acceptor DNA if the acceptor molecule has a 5' OH tail
complementary
1 S to that of the activated donor complex.
In accordance with the present invention, this reaction has been optimized for
joining PCR-amplified DNA fragments into plasmid vectors (See Figure 1). PCR
fragments are naturally good surrogate substrates for the topoisomerase I
religation
step because they generally have 5' hydroxyl residues from the primers used
for the
amplification reaction. The 5' hydroxyl is the substrate for the religation
reactions.
The use of vaccinia topoisomerase type I for cloning is described in detail in
copending US patent application serial number 08/358,344, filed 12/19/94,
incorporated by reference herein in its entirety.
The gene sequence being inserted into the expression vector can insert in
either the sense or antisense direction. Therefore, the invention method
provides for
verification of both the size and orientation of the insert to insure that the
gene
sequence will express the desired protein. Preferably, the insert plus vector
is utilized
in a standard bacterial transformation reaction and the contents of the
transformation
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plated onto selective growth media. Bacterial transformation and growth
selection
procedures are well known in the art and described in detail in, for example,
Ausubel,
et al, Short Protocols in Molecular Biology, 3rd ed. 1995.
Individual bacterial colonies are picked and grown in individual wells of a
multiwell microtiter plate containing selective growth media. An aliquot of
these
cells is used directly in a diagnostic PCR reaction. Primers for this reaction
are
designed such that only plasmids with correctly oriented inserts give
amplification
product. The amplified DNA is separated and visualized by SDS-PAGE gel
electrophoresis using standard protocols (see Ausubel, et al, Short Protocols
in
Molecular Biology, 3rd ed. 1995).
Performing the PCR reaction directly from the cultured cell lysates, rather
than
first preparing DNA from the bacteria, is a particular advantage of the
invention
method as it significantly reduces both the time needed to generate the
required data
and the cost of doing so.
1 S Once plasmids containing the gene sequence insert in the correct
orientation
have been identified, plasmid DNA is prepared for use in the transformation of
host
cells for expression. Methods of preparing plasmid DNA and transformation of
cells
are well known to those skilled in the art. Such methods are described, for
example,
in Ausubel, et al, supra.
Prokaryotic hosts are, generally, very efficient and convenient for the
production of recombinant proteins and are, therefore, one type of preferred
expression system. Prokaryotes most frequently are represented by various
strains of
E. coli. However, other organisms may also be used, including other bacterial
strains.
Recognized prokaryotic hosts include bacteria such as E. coli and those from
genera such as Bacillus, Streptomyces, Pseudomonas, Salmonella, Serratia, and
the
like. However, under such conditions, the polypeptide will not be
glycosylated. The
prokaryotic host selected for use herein must be compatible with the replicon
and
control sequences in the expression plasmid.
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Suitable hosts may often include eukaryotic cells. Preferred eukaryotic hosts
include, for example, yeast, fungi, insect cells, and mammalian cells either
in vivo, or
in tissue culture. Mammalian cells which may be useful as hosts include HeLa
cells,
cells of fibroblast origin such as VERO, 3T3 or CHOKI, HEK 293 cells or cells
of
lymphoid origin (such as 32D cells) and their derivatives. Preferred mammalian
host
cells include nonadherent cells such as CHO, 32D, and the like. Preferred
yeast host
cells include S. pombe, Pichia pastoris, S. cerevisiae (such as INVScl), and
the like.
In addition, plant cells are also available as hosts, and control sequences
compatible with plant cells are available, such as the cauliflower mosaic
virus 35S and
19S, nopaline synthase promoter and polyadenylation signal sequences, and the
like.
Another preferred host is an insect cell, for example the Drosophila larvae.
Using
insect cells as hosts, the Drosophila alcohol dehydrogenase or MT promoter can
be
used. Rubin, Science ~,Q:1453-1459, 1988). Alternatively, baculovirus vectors
can
be engineered to express large amounts of peptide encoded by a desire gene
sequence
in insects cells (Jasny, Science 2$:1653, 1987); Miller et al., In: Genetic
Engineering
(1986), Setlow, J.K., et al., eds., Plenum, Vol. 8, pp. 277-297).
In a further embodiment of the invention, there are provided libraries of
expressible gene sequences produced by the methods of the invention. As shown
in
more detail in the Examples presented below, such libraries comprise gene
sequences
from a variety of sources such as yeast, mammals (including humans), and the
like.
The present invention also features the purified, isolated or enriched
versions of the
expressed gene products produced by the methods described above.
Kits comprising one or more containers or vials containing components for
using the libraries of the present invention are also within the scope of the
invention.
Kits can comprise any one or more of the following elements: one or more
expressible gene sequences, cells which are or can be transfected with said
gene
sequences, and antibodies recognizing the expressed gene product or an epitope
tag
associated therewith. Cells suitable for inclusion in such a kit include
bacterial cells,
yeast cells (such as INVScl), insect cells or mammalian cells {such as CHO).
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In one embodiment, such a kit can comprises a detergent solution, preferably
the Trax~ lysing reagent (6% NP-40 and 9% Triton X-100 in 1X PBS). Also
included in the kit can be one or more binding partners, e.g., an antibody or
antibodies, preferably a pair of antibodies to the same expressed gene
product, which
preferably do not compete for the same binding site on the expressed gene
product.
In another embodiment, a kit can comprise more than one pair of such
antibodies or other binding partners, each pair directed against a different
target
molecule, thus allowing the detection or measurement of a plurality of such
target
molecules in a sample. In a specific embodiment, one binding partner of the
kit may
be pre-adsorbed to a solid phase matrix, or alternatively, the binding partner
and
matrix are supplied separately and the attachment is performed as part of the
assay
procedure. The kit preferably contains the other necessary washing reagents
well-
known in the art. For EIA, the kit contains the chromogenic substrate as well
as a
reagent for stopping the enzymatic reaction when color development has
occurred.
The substrate included in the kit is one appropriate for the enzyme conjugated
to one
of the antibody preparations. These are well-known in the art, and some are
exemplified below. The kit can optionally also comprise a target molecule
standard;
i.e., an amount of purified target molecule that is the target molecule being
detected or
measured.
In a specific embodiment, a kit of the invention comprises in one or more
containers: (1 ) a solid phase Garner, such as a microtiter plate coated with
a first
binding partner; (2) a detectably labeled second binding partner which binds
to the
same expressed gene product as the first binding partner; (3) a standard
sample of the
expressed gene product recognized by the first and second binding partners;
(4)
concentrated detergent solution; and (S) optionally, diluent.
The invention will now be described in greater detail by reference to the
following non-limiting examples.
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The following example illustrates the creation of a library of expressible
yeast
gene sequences.
Amplification -
6,032 yeast ORFs and a corresponding gene-specific primer of the 3' end of
each were obtained from Research Genetics (Huntsville, AL) in a 96-well
microtiter
plate format at a concentration of 0.3 ng/p,l. Each gene specific primer was
designed
to exclude the gene's stop codon. Since the templates each contain a common
sequence immediately 5' of the start ATG (5'-
GCAGTCCTGGAATTCCAGCTGACCACC) (SEQ ID NO:1 ), it was possible to
amplify each template with a common 5' primer.
5 p,l of ORF template was added to a fresh 96-well microtiter plate
(polycarbonate Thermowell Thinwall, Model M. Cat # 6511 ) using a 12 channel
pipetter. 6 pl of specific 3' primer solution (2 p.M) was added and the total
volume
per well brought to 30 p.l with PCR cocktail, immediately after which the
plate was
placed on ice. (PCR cocktail for 120 reactions - 720 pl SX Buffer J, 48 p,l
dNTPs
(SOmM stock), 12 ~,1 common 5' primer (1 p,g/pl stock), 48 pl Taq DNA
polymerise
(Boeringer-Mannheim or Promega, 5 units/pl), 1.92 p,l Pfu DNA polymerise
(Stratgene, cat. # 600153-81, 2.5 units/pl) and 1464 p,l distilled water. SX
Buffer J:
300 mM Tris (pH 9.5), 75 mM ammonium sulfate, 10 mM MgCl2). The rubber
Hybaid Micromat lid was washed by soaking in 0.1 M HCI, the rinsed for 2
minutes
with distilled water and dried completely before applying to the 96-well
plate.
The PCR reaction was performed using a Hybaid, Ltd. (Middlesex, LTK)
thermo-cycler according to the manufacturer's instructions. The conditions
used were
as follows: pre-melt step: 94° C x 4 min; melt step: 94° C x 30
sec, anneal step: 58°
C x 45 sec, extend step: 72° C x 3 min - repeated for 25 cycles; final
extension: 72°
C x 4 min; final block temperature set to room temp (approx. 22° C).
The plates were
stored at 4° C.
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Purification -
The plates were spun briefly at 1000 rpm, then 10 pl of 6X gel loading dye
was added to each well (6X gel Loading dye: 6 mM Tris (pH 8), 6 mM EDTA, 0.03%
Bromphenol Blue, 30% glycerol). The entire contents of each well were Loaded
onto
5 a 1 % low melt agarose (Invitrogen # 46-O 150) gel (plus ethidium bromide at
20 pl of
a 10 mg/ml solution added to 400 mls of agarose) in 1X TAE (SOX TAE = 2428
Tris
base, 57.1 ml glacial acetic acid, 100 ml 0.5 M EDTA, pH 8.0 per liter
(water)) and
run at I 10 - 120 volts for 1.25 to 1.5 hours. A IJV light box was used to
visualize the
amplification products and ensure that only correct-sized PCR products are
used in the
10 insertion step.
Insertion into expression vectors) -
The portion of each lane containing the amplified gene sequence was cut from
the gel and transferred to a well in a 96-well microtiter plate, melted on a
heat block
(75° C), and a portion of the melt mufti-channel pipetted into a 96-
well microtiter
15 plate (7 ~,i/well) containing one of two expression vectors: TOPO-adapted
pcDNA3.1 /GS or pYES2/GS (Invitrogen, Carlsbad, CA) previously digested with
HindIII. The plate was covered with parafilm and incubated at 37° C for
7 minutes.
Top 10 Chemically Competent Cells (Invitrogen) were added to each well (45
g,l/well,
O.D.=4.7), whereupon the plate was re-covered and incubated on ice for S
minutes.
The cells were then heat shocked on a 42° C block for 1 minute and
returned to ice for
1 minute. An aliquot of SOC medium was added to each well (150 ~tl, 20g
tryptone,
Sg yeast extract, O.Sg NaCI, 250 mM KCI, 20 ml 1M glucose/Liter), and the
plate was
incubated at 37° C for 90 to 120 minutes.
The contents of each well were plated onto a LB(1 Og tryptone, Sg yeast
extract, l Og NaCI per liter)/L .5% agar petrie plate containing the
appropriate selection
marker (ampicillin (50 pg/ml) for pYES2/GS and ZeocinT"' (25 pg/ml) for
pcDNA3.1/GS). The petrie plates were grown overnight at 37° C.
Verification of size and orientation -
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Contamination is a potentially serious problem in this step. Care should be
taken to guard against contaminating the process through airborne
contamination,
unsterile reagents or equipment, or well-to-well contamination.
Eight colonies were picked from each petrie plate and placed in eight
individual wells of a 96-well microtiter plate. Each well contained 100 pl of
2X LB
plus 100 ltg/ml ampicillin or 50 p,g/ml ZeocinTM as appropriate for the
expression
vector used. The plates were incubated overnight at 37° C.
The plates were spun briefly at 1000 rpm. The cells were stirred by pipetting
up and down in a pipetter, then 2 pl from each well was transferred to a
corresponding
well in a PCR reaction plate containing 28 pl/well PCR cocktail (PCR cocktail
for 840
reactions - 5040 p,l SX Buffer J, 336 ~tl dNTPs (SOmM stock), 84 p.l common 5'
primer (1 p,g/~tl stock, Dalton Chemical Lab. Inc, Ont. CAN), 84 p,l 3'
H6stopprevu
primer (1 pg/pl, Dalton Chemical Lab. Inc, Ont. CAN), 336 pl Taq DNA
polymerase
(Boeringer-Mannheim or Promega, 5 units/~1), and 17.64 mls distilled water.
H6stopprevu primer has the sequence 5' AAA CTC AAT GGT GAT GGT GAT GAT
GACC - 3') (SEQ ID N0:2).
The PCR reaction was run essentially as described above with the following
cycle: pre-melt step: 94° C x 10 min; melt step: 94° C x 1 min,
anneal step: 67° C x
1 min, extend step: 72° C x 3 min - 35 cycles; final extension:
72° C x 4 min; final
block temp set to room temp (approximately 22° C). The plates were spun
briefly at
100 rpm and 6 pl of 6X gel loading dye added to each well. Samples were run on
a
1 % agarose gel which was subsequently stained with ethidium bromide. Only
plasmids with correctly oriented inserts give an amplification product in this
step.
The location of the positive clones was entered into a database and a
spreadsheet of positive clones generated. The spreadsheet was downloaded onto
a
Qiagen BioRobot 9600TM to direct the re-racking of the positive cultures into
deep-
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well culture blocks. Essentially, a single positive culture for each clone was
grown
and used to prepare plasmid DNA according to the Quia-Prep Turbo protocol.
CHO cells were transfected with the prepared plasmid DNA using the Pfx-6
PerFect Lipid system (Invitrogen, Cat #T930-16). Yeast cells (INVScl) were
transfected using the S.C. EasyComp Transformation kit (Invitrogen, Cat #K5050-
0 i ). Expression was verified by Western blot using anti-VS antibody to
detect the
epitope tag. A total of 558 clones expressing a correct protein were obtained
after a
single pass.
The following example illustrates the construction of a library of expressible
human gene sequences using the method of the invention. Primers were
constructed
based on sequences of human genes available from GenBank.
Fetal human heart tissue was obtained from the International Institute for the
Advancement of Medicine (IIAM). Poly A+ mRNA was isolated using the
1 S FastTrackTM 2.0 Kit (Invitrogen, Carlsbad, CA) according to the
manufacturer's
instructions. The mRNA was converted to first-strand cDNA using a cDNA Cycle~
Kit (Invitrogen) using the oligo dT primer provided and the protocols
suggested. A
single cDNA synthesis reaction was split into 12 separate wells of a 96-well
PCR
amplification plate, and PCR amplifications were performed using specific
primer
sets, essentially as described above, with the exception that the ratio of Taq
to Pfu was
50:1 in the initial amplification (final conc. 2 U Taq:0.04 U Pfu/well).
Primers were synthesized using a Primerstation 960 (Intelligent Automation
Systems, Inc.) used according to the manufacturer's instructions and were
designed
from sequences downloaded from Unigene and sent directly to the synthesizer.
Approximately 15 nMoles of each primer, having an average length of 25
basepairs,
was synthesized in a 96-well format. After synthesis, the primers were cleaved
from
the supports, deprotected and dried in the same 96-well format (see
manufacturer's
instructions).
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The amplified gene sequences were purified and inserted into the
pcDNA3.l/GS expression vector essentially as described above. The expression
vectors containing sequences verified to be in the correct orientation were
transfected
into CHO cells in 96-well deep-well blocks using the Pfx-6 PerFect Lipid
system
(Invitrogen, Cat #T930-16). Cell lysates were made 48 hours after
transfection, and
the lysates were separated by SDS-PAGE and analyzed by Western blot according
to
standard protocols using an anti-VS epitope tag Mab/horseradish peroxidase
conjugate. Table 1 lists the human proteins successfully expressed using this
methodology. A total of 66 clones expressing a correct protein, out of 118,
were
obtained after a single pass.
Table 1 - Human ORFs
Plate NumberAccession Description PredictedActual
Number
Size Size
M235 C7 H-A06977 albumin
67.1 67.OkDa
E1 H-AB002391 Human mRNA for ICIAA039368
09
. 6g
gene, complete cds
H3 H-AB006969 Homo Sapiens hGAA 68.42 70
1 mRNA,
complete cds
E2 H-AB007875 Homo Sapiens KIAA041551.48 51
mRNA,
complete cds
D 1 H-AB007887 Homo Sapiens KIAA042766.55 70
mRNA,
complete cds
M421 D6 H-AB010710 Homo Sapiens mRNA 30.14 45.OkDa
for lectin-
- like oxidized LDL
receptor,
complete cds
G3 H-AD001528 Homo Sapiens spenmidine40.37 40
aminopropyltransferase
mRNA,
complete cds
BS H-AE000659 Homo Sapiens T-cell 12.39 16
receptor
alpha delta locus
from bases
250472 to 501670 (section
2 of 5)
of the C
E2 H-AF004022 Homo Sapiens protein 38.28 44
kinase
mRNA, complete cds
M428 C1 H-AF004231 Homo sapiens 65.78 70.OkDa
monocyte/macrophage
Ig-related
receptor MIR-10 (MIR
cl-10)
mRNA, complete cds
AS H-AF004327 Homo Sapiens angiopoietin-254.67 60
mRNA, complete cds
C1 H-AF006501 Homo Sapiens chromosome14.08 24
22
cosmid clone c1155,
RNA
polymerase II subunit
14.4 kDa
(POLRF) gene, complete
cds
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H4 H-AF008936 Homo sapiens syntaxin-16B35.75 47
m
RNA, complete c
ds
HS H-AF009243 _ 22.33 36
_
Homo Sapiens proline-rich
Gla
protein 2 (PRGP2)
mRNA,
complete cds
M462 D6 H-AF013249 Homo Sapiens leukocyte-31.68 40.OkDa
associated Ig-like
receptor-1
(LAIR-1) mRNA, complete
cds
A 1 H-AF013512 untitled 53.02 53
A3 H-AF013970 Homo Sapiens MTGB-like66.55 70
protein
(MTGRI) mRNA, complete
cds
M467 A7 H-AF014807 Homo sapiens 23.54 29.OkDa
phosphatidylinositol
synthase
(PIS) mRNA, complete
cds
D2 H-AF015257 Homo sapiens flow-induced41.36 40
endothelial G protein-coupled
receptor (FEG-1)
mRNA,
complete cds
M422 B5 H-AF017307 Homo sapiens Ets-related40.92 49.OkDa
transcription factor
(ERT) mRNA,
complete cds
A6 H-AF017656 Homo Sapiens G protein38.94 48
beta 5
subunit mRNA, complete
cds
EI H-AF017995 Homo sapiens 3-phosphoinositide61.27 52
dependent protein
kinase-1
(PDK1) mRNA, complete
cds
G 1 H-AF019612 Homo sapiens S2P 57.2 57
mRNA,
complete cds
D3 H-AF020591 Homo sapiens zinc 78.76 74
forger protein
mRNA, complete cds
A7 H-AF022385 Homo Sapiens apoptosis-related23.43 33
protein TFAR15 (TFAR15)
mRNA, complete cds
H6 H-AF024714 Homo sapiens interferon-37.84 48
inducible protein
(AIM2) mRNA,
complete cds
B1 H-AF025527 Homo Sapiens leucocyte48.4 47
immunoglobulin-like
receptor-4
(LIR-4) mRNA, complete
cds
M424 B4 H-AF025532 Homo Sapiens leucocyte49.39 59.OkDa
immunoglobulin-like
receptor-5
(LIR-5) mRNA, complete
cds
H5 H-AF026071 Homo Sapiens soluble30.58 50
death
receptor 3 beta (DR3)
mRNA,
complete cds
-
M428 A1 H-AF026273 Homo sapiens interleukin-165.01 68.OkDa
receptor-associated
kinase-2
mRNA, complete cds
B6 N-AF026293 Homo Sapiens chaperonin58.96 58
containing t-complex
polypeptide
1, beta subunit (Cctb)
mRNA,
complete cds
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B5 H-AF026548 Homo sapiens branched45.43 50
chain
alpha-ketoacid dehydrogenase
kinase precursor,
mRNA, nuclear
gene encoding mitochondrial
protein, complete
cds
B2 H-AF027204 Homo Sapiens putative21.78 27
tetraspan
transmembrane protein
L6H
(TM4SF5) mRNA, complete
cds
M426 D3 H-AF028008 Homo Sapiens SP1-like56.43 64.OkDa
zinc
forger transcription
factor SLP
mltNA, complete cds
B 1 H-AF029232 Homo Sapiens calpamodulin70.62 70
(CalpM) mRNA, complete
cds
M422 A7 H-AF029761 Homo sapiens decoy 42.57 50.OkDa
receptor 2
mltNA, complete cds
M477 F3 H-AF029893 Homo Sapiens i-beta-1,3-N-45.76 50.OkDa
acetylglucosaminyltransferase
mRNA, complete cds
C5 H-AF032437 Homo sapiens mitogen 51.92 SO
activated
protein kinase activated
protein
kinase gene, complete
cds
M416 F3 H-AF035824 Homo sapiens vesicle 25.63 36.OkDa
soluble
NSF attachment protein
receptor
(VTI1) mRNA, complete
cds
F3 H-AF037335 Homo Sapiens carbonic39.05 39
anhydrase
precursor (CA 12)
mIZNA,
complete cds
G I H-AF039019 Homo sapiens zinc 87.45 87
forger DNA
binding protein 89
kDa (ZBP-89)
mIZNA, complete cds
G 1 H-AF039136 Homo sapiens Fas binding81.51 98
protein
(hDaxx) m)ZIVA, complete
cds
A7 H-AF0407U5 Homo sapiens putative31.57 41
tumor
suppressor protein
unspliced form
(Fus-2) m)ZNA, complete
cds
M469 F1 H-AF040958 Homo sapiens lysosomal45.76 46.OkDa
neuraminidase precursor,
mltNA,
complete cds
G2 _ Homo sapiens Shab-related54.12 64
H-AF043472
delayed-rectifier
K+ channel
alpha subunit (Kv9.3)
mIZNA,
complete cds
E2 H-AJ001340 Homo Sapiens mRNA 52.36 60
for U3
snoRNP associated
55 kDa
protein
G 1 H-D00096 Transtyretin (prealbumin)16.28 20
C4 H-D00408 Cytochrome P450 IIIA755.44 64
(P450-
HFLa)
M302 E7 H-D00682 cofilin 18.37 30
M383 G2 H-D00726 ferrochelatase 46.64 50.OkDa
M383 C3 H-D00760 proteasome, subunit 25.85 34.OkDa
HC3
M305 B4 H-D00761 proteasome, subunit 26.62 33
HC5
M266 F7 H-D00763 proteasome, subunit 28.82 33
HC9
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E2 H-D00860 Phosphoribosyl pyrophosphate35.09 47
synthetase subunit
I
2 i 5- H-D 10522 human mRNA for 80K-L 3 S 36.59
I 3 protein
M423 FS H-D 11086 Interleukin 2 receptor40.7 45.OkDa
gamma
chain
M248 D2 H-D11094 positive modulator 47.74 40.0kDa
of HIV tat-
mediated transactivation
G3 H-D11428 Peripheral myelin 17.71 17
protein 22
M424 D3 H-D13168 Human gene for endothelin-B48.73 48.OkDa
receptor (hET-BR)
M271 B8 H-D13315 glyoxalase I, 20.35 34.OkDa
LACTOYLGLUTATHIONE
LYASE. CATALYZES THE
CONVERSION OF
HEMIMERCAPTAL, FORMED
FROM METHYLGLYOXAL
AND GLUTATHIONE, TO
S-
LACTOYLGLUTATHIONE.
M306 F1 H-D13627 hypothetical protein 60.39 90
(GB:D13627)
M248 D1 H-D13630 hypothetical protein 46.2 49
(GB:D13630) , Human
mRNA for
KIAA0005 gene, complete
- cds
M270 DS H-D13634 hypothetical protein 34.65 42.OkDa
(GB:D 13634)
M250 D2 H-D13642 hypothetical protein 44 48.OkDa
(GB:D13642),Human
mRNA for
KIAA0017 gene, complete
cds
M250 E6 H-D13748 translation initiation44.77 49.OkDa
factor 4A
M305 C3 H-D13892 carboxyl methyltransferase,25.19 34
aspartate
D1 H-D13900 enoyl-Coenzyme A hydratase,32.01 58
short chain, mitochondria)
E1 H-D14446 Human HFREP-1 mRNA 34.43 40
for
unknown protein, complete
cds
167-14 H-D14497 H.sapiens (Ewing's 51.44 64
sarcoma cell
line) mRNA encoding
open
reading frame
M266 D2 H-D14520 basic transcription 24.2 33.OkDa
element-
binding protein 2
M318 D2 H-D14658 hypothetical protein 13.64 17
(GB:D14658)
D2 H-D14661 Human mRNA for KIAA010516.72 28
gene, complete cds
M236 E2 H-D14662 HYPOTHETICAL 29.5 24.75 36.OkDa
KD
PROTEIN IN UBP13-KIPI
INTERGENIC REGION
[Saccharomyces cerevisiaej
M271 G6 H-D14695 hypothetical protein 43.12 SO.OkDa
( GB:D14695), Human
mRNA for
KIAA0025 gene, complete
cds.
M311 A3 H-D14696 hypothetical protein 25.74 30.OkDa
( GB: D 14696)
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H3 H-D14697 Farnesyl diphosphate 46.2 SS
synthase
(farnesyl pyrophosphate
synthetase,
dimethylallyltranstransferase,
geranyltranstransferase)
M271 E7 H-D1470S catenin, alpha 2(E). 99.77 110
Catenin
(cadherin-associated
protein),
alpha 1 (102kD). ASSOCIATES
WITH THE CYTOPLASMIC
DOMAIN OF A VARIETY
OF
CADHERINS.
M236 A6 H-D14811 hypothetical protein 30.25 42
(GB:D 14811 )
M2S0 A3 H-D14812 hypothetical protein
(GH:D14812), Human
mRNA for
KIAA0026 gene, complete
cds
AS H-D14874 Human mRNA for 20.46 33
adrenomedullin, complete
cds
F3 H-D14887 Human mRNA for TFIIA-42,41.47 SO
complete cds
M250 H6 H-D 16234 phospholipase C, alphaSS.66 S6.OkDa
,
PROBABLE PROTEIN
DISULFIDE ISOMERASE
ER-
60 PRECURSOR [Homo
Sapiens]
M30S B1 H-D16480 enoyl-CoA hydratase/3-84.04 g4
hydroxyacyl-CoA dehydrogenase
trifunctional protein,
alpha-
subunit, mitochobdrial
M271 G2 H-D16481 3-ketoacyl-CoA thiolase,
beta
subunit, mitochodrial,
Hydroxyacyl-Coenzyme
A
dehydrogenase/3-ketoacyl-
Coenzyme Athiolase/enoyl-
Coenzyme A hydratase
(trifunctional protein),
beta
subunit
H1 H-D16626 Histidine ammonia-lyase72.38 64
A2 H-D17532 Human mRNA for RCK, 52.03 53
complete cds
M266 F4 H-D17SS4 DNA-binding protein 31.79 38
TAX
M248 A3 H-D2123S xeroderma pigmentosum40.04 SS
group C
repair complementing
protein
HHR23A
M235 E1 H-D21261 SM22-ALPHA HOMOLOG, 22 31
hypothetical protein
(GB:D21261 )
M311 E 1 H-D21262 hypothetical protein 77.950 63
(GB:D21262)
M466 B4 H-D218S3 Human mRNA for KIAA011145.32 49.OkDa
gene, complete cds
M311 H3 H-D23660 ribosomal protein 47.08 47
L4
M419 E 1 H-D26309 human mRNA for LIMK 71.240 75.OkDa
(LIM
kinase)
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M271 B9 H-D26362 hypothetical protein79.97 70
(GB:D26362), Human
mItNA for
KIAA0043 gene, complete
cds
M361 H2 H-D26598 proteasome, subunit 22.66 33.OkDa
HsClO-II
M302 G4 H-D26599 proteasome, subunit 22.22 34
HsC7-I
G1 H-D26600 Human mRNA for proteasome29.15 36
subunit HsN3, complete
cds
G9 H-D28540 hypothetical protein,44.77 60
CDC10
homolog
M266 AS H-D29011 proteasome, subunit 22.99 23
X
M236 F3 H-D29012 Proteasome (prosome,26.4 32.OkDa
macropain)
delta subunit, beta
type, 6
C1 H-D30037 Human mItNA for 29.92 38
phosphatidylinositol
transfer
protein (PI-TPbeta),
complete cds
M250 H4 H-D30655 translation initiation44.88 45.OkDa
factor 4AII,
and ribosomal binding
protein
167-26 H-D30742 human mltNA for cahnodulin-52.10 55
dependent protein
kinase IV
M236 A4 H-D31767 hypothetical protein18.59 30
(GB:D31767), Human
mItNA for
KIAA0058 gene, complete
cds
E1 H-D31883 Human mltNA for KIAA005950.93 64
gene, complete cds
G2 H-D32129 MHC class I protein 40.26 50
HLA-A
M422 A6 H-D37965 Human mltNA for PDGF41.36 45.OkDa
receptor
beta-like tumor suppressor
(PRLTS), complete
cds
M305 H4 H-D38047 26S proteasome regulatory28.340 34.OkDa
subunit P31
M423 B2 H-D38081 Thromboxane A2 receptor37.84 45.OkDa
M317 D3 H-D38305 ErbB-2 transducer 38.06 49
M270 A8 H-D38583 calgizzarin, Human 11.66 12
mRNA for
calgizzarin, complete
cds
M270 A6 H-D42038 hypothetical protein15.29 27
(GB:D42038), Human
mItNA for
KIAA0087 gene, complete
cds
M318 F3 H-D42085 hypothetical protein90.2 100
(GB:D42085)
M311 C2 H-D43642 YL-1 protein homolog40.15 36
E I H-D45213 Human mItNA for zinc12.87 20
forger
protein, complete
cds
M236 B2 H-D45248 proteasome activator26.4 38
hPA28,
subunit beta , may
be cell
adhesion protein
H3 H-D45887 Human mRNA for calmodulin,16.5 20
complete cds
166-3 H-D45906 human mltNA for LIMK-270 70.25
A7 H-D49357 Human mRNA for S- 43.56 51
adenosylmethionine
synthetase,
complete cds
CS H-D49489 Human mltNA for protein48.51 54
disulfide isomerase-related
protein P5, complete
cds
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M482 E2 H-D49958 Human fetus brain 30.69 32.OkDa
mRNA for
membrane glycoprotein
M6,
complete cds
M305 GS H-D50063 proteasome, subunit 35.75 39
p40
M250 B6 H-D50310 cyclin I, Human mltNA41.58 47
for cyclin
I, complete cds
E3 H-D50419 Homo Sapiens mRNA 78.32 64
for OTK 18,
complete cds
M298 B1 H-D50495 transcription elongation33 33.OkDa
factor h-
SII-Tl (GB:D50495)
M302 A3 H-D50840 ceramide glucosyltransferase43.45 44
167-40 H-D50863 human mRNA for TESK1 68.9 70
3
166-28 H-D50927 human myeloblast mRNA60.46 64
for
KIAA0137 gene
D 1 H-D63521 Homo sapiens mRNA 16.72 16
for LECT2
precursor, complete
cds
M302 AS H-D78134 glycine-rich binding 19.03 30.OkDa
protein CIRP
M313 ES H-D78275 proteasome subunit 42.9 48.OkDa
p42
B3 H-D79205 Human mRNA for ribosomal5.72 10
protein L39, complete
cds
A4 H-D79206 Human gene for ryudocan21.89 33
~ core
protein, exonl-5,
complete cds
A 1 H-D80008 Human mRNA for KIAAO 21.67 32
I 86
gene, complete cds
M298 H4 H-D83004 ubiquitin-conjugating16.83 32.OkDa
enryme E2
similar to Drosophila
bendless
gene product
C3 H-D83702 Human brain mRNA for 64.57 64
photolyase homolog,
complete
cds
M306 A1 H-D83735 neutral calponin 34.1 34
OkDa
H2 H-D86322 Homo Sapiens mRNA 67.21 .
for 64
cahnegin, complete
cds
B 1 H-D86979 Human mRNA for KIAA022682.72 82
gene, complete cds
169-16 H-D87116 duai specificity mitogen-activated38.24 42
protein kinase kinase
- 3
166-27 H-D87119 human cancellous bone37.80 40
osteoblast
mRNA for GS3955
E2 H-D88308 Homo sapiens mRNA 68.31 64
for very-
long-chain acyl-CoA
synthetase,
complete cds
166-26 H-D89077 human mRNA for Src-like30.43 38
adapter protein
M440 H2 H-D89479 Homo Sapiens mRNA 32.67 38.OkDa
for ST1B2,
complete cds
H1 H-D90086 Human pyruvate dehydrogenase39.6 35
(EC 1.2.4.1) beta
subunit gene,
exons I-10
M362 FI H-D90209 DNA-binding protein 38.72 48.OkDa
TAXREB67
M316 B2 H-J00068 actin, alpha 1, skeletal41.58 50
muscle
M250 B2 H-J00194 major histocompatibility28.05 36.OkDa
complex,
MHC class II, DR alpha
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G2 H-100212 Interferon, alpha 20.9 30
~ ~ 21
G1 H-100287 Human pepsinogen gene42.79 48
M298 C2 H-102611 apolipoprotein D 20.9 3l.OkDa
M266 C4 H-102683 ADP/ATP carrier protein32.89 36
M383 H2 H-102685 plasminogen activator45.76 SO.OkDa
inhibitor,
placenta
167-3 H-102853 "casein kinase II, 43.08 50
alpha chain"
E3 H-102854 Human 20-kDa myosin 19.03 31
light
chain (MLC-2) mRNA,
complete
cds
M248 F3 H-102874 fatty-acid-binding 14.63 17
protein 4,
adipocyte, LIPID TRANSPORT
PROTEIN IN ADIPOCYTES
M235 DS H-102939 antigen 4F2, heavy 58.3 58
chain
C3 H-102943 Corticosteroid binding44.66 50
globulin
M248 F2 H-102966 adenine nucleotide 32.78 33
translocator 1
(skeletal muscle)
[ANTI),
CATALYZES THE EXCHANGE
OF ADP AND ATP ACROSS
THE MITOCHONDRIAL
INNER MEMBRANE.
EI H-102982 Glycophorin B 10.12 20
167-91 H-103075 "protein kinase c 58.04 98
substrate, 80 kD
protein heavy chain"
M266 A3 H-103191 profilin 1 15.51 l7.OkDa
M248 H4 H-103231 glucose-6-phosphate 56.76 51
dehydrogenase [G6PDj
M266 F2 H-103459 LEUKOTRIENE A-4 67.32 64
HYDROLASE [Homo sapiens]
A2 H-103460 Prolactin-induced 16.17 26
protein
M271 ES H-103799 laminin receptor 1, 32.56
Laminin
receptor (2H5 epitope).
40S
RIBOSOMAL PROTEIN
SA
[Homo sapiensj.
M440 A4 H-103890 Human pulmonary surfactant21.78 30.OkDa
protein C (SP-C) and
pulmonary
surfactant protein
C 1 (SP-C 1 )
genes, complete cds
M271 D8 H-103934 NAD(P)H menadione 30.25 38
oxidoreductase 1,
dioxin-
inducible. INVOLVED
IN
DETOXICATION PATHWAYS.
M271 A8 H-104031 trifunctional enzyme 102.96 117.OkDa
(GB:J04031). C-1-
TETRAHYDROFOLATE
SYNTHASE, CYTOPLASMIC
[Homo sapiensj
M305 F6 H-104046 calmodulin 3 [CALM3j 16.5 20
M305 G7 H-104071 cytotoxic T-lymphocyte-27.28 38
associated serine
esterase 1
(cathepsin G-like
1, granzyme B)
[CTLA 1
M311 D2 H-104183 lysosomal-associated 44.99 47
membrane
protein 2
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M300 F4 H-104205 Sjogren syndrome antigen44.99 S 1.OkDa
B
M416 G8 H-104430 Acid phosphatase 5, 35.64 45.OkDa
tartrate
resistant
BI H-104501 Glycogen synthase 81.18 81
1 (muscle)
M313 BS H-104543 synexin 51.37 51
B1 H-104605 Peptidase D 54.34 55
M250 C6 H-104615 small nuclear ribonucleoprotein26.51 34.OkDa
SM-D, ROLE IN THE
PRE-
mRNA SPLICING OR IN
SNRNPSTRUCTURE.
M248 E2 H-104964 steroid sulfatase 64.24 60.OkDa
(microsomal)
[STS]
M250 A7 H-105249 replication protein 29.81 36.OkDa
A, 32 kDa
subunit , REQUIRED
FOR SV
40 DNA REPLICATION
IN
VITRO, RP-A IS SINGLE-
STRANDED DNA-BINDING
PROTEIN.
F1 H-105272 IMP (inosine monophosphate)56.65 51
dehydrogenase 1
169-15 H-105401 "creatine kinase, 50 46.16
sarcoraeric
mitochondria) precursor"
M266 E4 H-105448 RNA polymerase II, 30.36 35
subunit B33 OkDa
M305 C2 H-K00558 tubulin, alpha kI 49.72 .
[TUBA*] 52
OkDa
M416 H7 H-K01571 Human T-cell receptor34.43 .
active 36.OkDa
beta-chain, mRNA from
cell line
MOLT-3, complete cds
M311 E4 H-K01763 haptoglobin 38.28 47
OkDa
GS H-K02100 Human ornithine 39.05 .
47
transcarbamylase (OTC)
mRNA,
complete coding sequence
M302 DS H-K02574 purine nucleoside 31.9 36
phosphorylase OkDa
169-39 H-K02581 "thymidine kinase, 34 .
cytosolic" 25
81
M248 E4 H-K03020 phenylalanine hydroxylase49.83 .
[PAH] 50
M556 B3 H-K03191 Cytochrome P450, subfamily56.43 53.OkDa
I
(aromatic compound-inducible),
potypeptide 1
N2 H-L00190 Antithrombin III 51.15 55
169-62 H-L01087 "protein kinase c, 80 77
theta type" 7
M3 I 8 C2 H-LO I 124 ribosomal protein 16.72 ,
S 13 28
M313 F1 H-L02321 glutathione S-transferase24.09 28
MS
M305 ES H-L02426 protease 26S, regulatory48.51 53
subunit 4
M302 D4 H-L02547 cleavage stimulation 47.52 SO.OkDa
factor, 50
kDa subunit
M266 H7 H-L02648 transcobalamin II 47.08 48.OkDa
E2 H-L02932 Human peroxisome proliferator51.59 59
activated receptor
mRNA,
complete cds
M270 A1 H-L03380 gonadotropin-releasing36.19 36
hormone
receptor [GRHR], THIS
RECEPTOR MEDIATES
ITS
ACTION BY ASSOCIATION
WITH G PROTEINS
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M270 H 1 H-L03411 RD protein [RDBP], 41.91 59.OkDa
Radin blood
group
D3 H-L03426 Human XE7 mRNA, complete42.46 45
alternate coding regions
B1 H-L03785 Myosin, lightpolypeptide19.14 32
5,
regulatory
A7 H-L04483 ribosomal protein 9.24 34
S21
M416 B2 H-L05147 Human dual specificity20.46 30.OkDa
phosphatase tyrosine/serine
mRNA, complete cds
215-38 H-L05624 dual specificity mitogen-activated50 43.30
protein kinase kinase
1
M271 D4 H-L06132 anion channel, voltage-gated,31.24 37
isoform I. FORMS A
CHANNEL
THROUGH THE CELL
MEMBRANE, THAT ALLOWS
DIFFUSION FROM SMALL
HYDROPHYLIC MOLECULES.
169-27 H-L06139 tyrosine-protein kinase125 123.7
receptor
TIE-2 precursor
H1 N-L06147 Human (clone SY11) 68.31 68
golgin-95
mRNA, complete cds
M250 A 1 H-L06419 procollagen-lysine, 80.08 80.OkDa
2-oxoglutarate
5-dioxygenase (lysine
hydroxylase) [PLOD]
M236 F6 H-L06498 ribosomal protein 13.2 23.OkDa
S20
M318 D1 H-L06499 ribosomal protein 10.23 27
L37a
M270 D1 H-L07414 CD40 antigen ligand 28.82 36
[CD40LG],
NVOLVED IN
IMMUNOGLOBULIN CLASS
SWITCHING.
M298 A6 H-L07548 aminoacylase 1 44.99 52.OkDa
M424 C3 H-L07592 Human peroxisome proliferator48.62 48.OkDa
activated receptor
mRNA,
complete cds
M298 G6 H-L07633 proteasome (prosome, 27.5 33.OkDa
macropain)
activator subunit
1 (PA28 alpha)
[PSME 1 ]
M318 B 1 H-L08096 CD70 antigen (CD27 21.34 28
ligand)
[CD70]
D2 H-L08187 cytokine receptor 25.3 42
EBI3
M313 F4 H-L08850 amyloid, non-A beta 15.51 3l.OkDa
component,
Alzheimer's disease
M426 E1 H-L08895 MADS box transcription52.14 60.OkDa
enhancer
factor 2, polypeptide
C (myocyte
enhancer factor 2C)
M266 A8 H-L09235 ATPase, vacuolar 67.98 64.OkDa
M266 D 1 H-L09604 differentiation-dependent16.83 17.OkDa
intestinal membrane
A4 protein
(Homo sapiens)
M317 CI H-L10338 sodium channel, voltage-gated,24.09 24
type I, beta polypeptide
[SCN1B]
M317 E1 H-L10717 tyrosine-protein kinase68.270 68.OkDa
ITK/TSK
M300 BS H-L10820 formyl peptide receptor38.61 3?
1 [FPRI]
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M312 A4 H-L10838 pre-mRNA splicing 18.15 3l.OkDa
factor SRp20
M300 AS H-L10918 chemokine (C-C) receptor39.16 30
1
[CMKBRI]
M311 F2 H-L11245 complement component 27.83 30
4-binding
protein, beta
M266 B7 H-L11353 neurofibromatosis 65.56 63.OkDa
2 (bilateral
acoustic neuroma)
[NF2)
M311 B3 H-L11667 cyclophilin 40 40.81 SO.OkDa
215-49 H-L11695 serine/threonine-protein64 55.40
kinase
receptor R4 precursor
M466 C2 H-L 11931 Human cytosolic serine53.24 56.OkDa
hydroxymethyltransferase
(SHMT) mRNA, complete
cds
M271 B7 H-L12168 ADENYLYL CYCLASE- 52.36 60.OkDa
ASSOCIATED PROTEIN
1
[Homo Sapiens)
M416 D4 H-L12964 Interleukin-activated28.16 38.OkDa
receptor,
homolog of mouse Ly63
B3 H-L13203 Human HNF-3/fork-head38.72 49
homolog-3 HFH-3 mRNA,
complete cds
D2 H-L13744 Human AF-9 mRNA, complete62.59 63
cds
167-8 H-L13943 glycerol kinase 60 57
71
M311 G3 H-L13974 leucinezipperprotein 41.14 .
51
(GB:L13974)
M271 HS H-L13977 LYSOSOMAL PRO-X 54.67 57
CARBOXYPEPT)DASE
PRECURSOR [Homo sapiens).
M270 G2 H-L 14283 protein kinase C, 65.23 98
zeta [PRKCZ),
SERINE- AND THREONINE-
SPECIFIC ENZYME.
M235 A3 H-L14286 antioxidant protein, 21.89 32
thiol-specific OkDa
M426 H3 H-L14778 Protein phosphatase 57.42 .
3 (formerly 60.OkDa
2B), catalytic subunit,
alpha
isoform (calcineurin
A
alpha){alternative
products}
B4 H-L 15702 complement factor 84.15 100
B
M426 A4 H-L 16794 Human transcription 57.42 60.OkDa
factor
(MEF2) mRNA, complete
cds
215-25 H-L 16862 g protein-coupled 70 63.4
receptor kinase
GRK6
167-74 H-L16991 thymidylate kinase 36 23.39
169-3 H-L18964 "protein kinase c, 80 64
iota type" 64
M305 E2 H-L18972 hypothetical protein 75.24 .
(GB:L18972) 7g
M426 D4 H-L19067 Human NF-kappa-B transcription59.18 63.OkDa
factor p65 subunit
mRNA,
complete cds
215-26 H-L19268 Homo Sapiens myotonic70 68.71
dystrophy associated
protein
kinase mRNA
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M271 El H-L19297 carbonic anhydrase 33.66 42
V [CAS],
Mitochondrial carbonic
anhydrase. REVERSIBLE
HYDRATATION OF CARBON
DIOX)DE.
M298 G4 H-L19437 transaldolase 37.18 39
OkDa
M423 C4 H-L 19593 Interleukin 8 receptor,39.71 .
beta 41
OkDa
G1 H-L19686 Homo sapiens macrophage12.76 .
13
migration inhibitory
factor (MIF)
gene, complete cds
G2 H-L19739 metallopanstimulin 9.35 32
1
M302 E3 H-L19871 activating transcription20.02 36
factor 3 OkDa
167-86 H-L20422 14-3-3 protein eta 34 .
27
1 3
M440 B2 H-L20492 Human gamma-glutamyl 24.86 .
35.OkDa
transpeptidase mRNA,
complete
cds
M315 B H-L20688 GDP-dissociation inhibitor22.22 32
1
protein rhoA
M271 H3 H-L20941 ferritin, heavy polypeptide.20.24 32
FERRITIN IS AN
INTRACELLULAR
MOLECULE THAT STORES
IRON IN A SOLUBLE,
NONTOXIC, READILY
AVAILABLE FORM.
M235 B7 H-L21893 Na+/taurocholate cotransporter,
STRICTLY DEPENDENT
ON
THE
F 1 H-L21934 Sterol O-acyltransferase60.61 60
{acyl-
Coenzyme A: cholesterol
acyltransferase)
C2 H-L22075 Human guanine nucleotide41.58 50
regulatory protein
(G13) mRNA,
complete cds
169-18 H-L22206 vasopressin v2 receptor60 58.00
M421 AIO H-L22214 Human adenosine A1 35.97 38.OkDa
receptor
(ADORA 1 ) mRNA exons
1-6,
complete cds
M424 Fl H-L23959 Homo Sapiens E2F-related45.21 53.OkDa
transcription factor
(DP-1)
mRNA, complete cds
C2 H-L24498 Human gadd45 gene, 18.26 28
complete
cds
M302 E2 H-L25080 proto-oncogene rhoA, 21.34 31
multidrug
resistance protein
M270 B8 H-L25081 guanine nucleotide-binding21.34 30
and
transforming protein
rhoC,
Aplysia ras-related
homolog 9
M236 E3 H-L25085 Sec61 complex, beta 10.67 19
subunit,
PROTEIN TRANSLOCATION
IN THE ENDOPLASMIC
RETICULUM
167-85 H-L25610 cyclin-dependentkinaseinhibitor32 18.11
1
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B2 H-L2561 cyclin-dependent kings18.1 40
'~mhib'rtor
1
__
M297 H2 H-L26232 cathepsin A/phos transfer54.34 64.OkDa
protein
167-4 H-L26318 stress-activated protein52 42.31
kinase
JNK I
M428 Fl H-L27586 Human TR4 orphan receptor67.76 67.OkDa
mRNA, complete cds
M302 ES H-L27711 protein phosphatase 23.43 28
KAPI
M250 A6 H-L28010 Homo Sapiens HnRNP
F protein
mRNA, complete cds,
F1 H-L28821 Alpha mannosidase 87.67 87
II isozyme
167-89 H-L28824 tyrosine-protein kinase70 69.92
SYK
M298 E6 H-L28997 ADP-ribosylation factor-like20.02 33.OkDa
gene
1
D4 H-L29219 Homo sapiens clkl 53.35 60
mRNA,
complete cds
169-63 H-L29222 Homo Sapiens clkl 25 15
mRNA 0 3
M429 B3 H-L29277 Signal transducer 84.81 .
and activator of 88.OkDa
transcription 3 (acute-phase
response factor)
C 1 H-L29433 Human factor X (blood53.79 64
coagulation factor)
gene
G3 H-L31860 Glycophorin A 16.61 26
D1 H-L31881 Nuclear factor I/X 48.62 4g
(CCAAT-
binding transcription
factor)
169-13 H-L31951 human protein kinase 55 46.71
(.1NK2)
mRNA
A 1 H-L32179 Arylacetamide deacetylase44 50
(esterase)
B2 H-L33404 Human stratum corneum27.94 36
chymotryptic enzyme
mRNA,
complete cds
M312 D3 H-L33799 procollagen C-proteinase49.5 S I .OkDa
enhancer
169-77 H-L33801 human protein kinase 55 46.27
mRNA
GSK-3
M305 D6 H-L34041 L-glycerol-3-phosphate:NAD+38.5 42.OkDa
oxidoreductase
B4 H-L34355 Homo sapiens (clone 42.68 47
p4) 50 kD
dystrophin-associated
glycoprotein mRNA,
complete
cds
M297 B3 H-L35013 spliceosomal protein 46.75 52.OkDa
SAP 49
167-32 H-L35253 human CSaids binding 52 39.67
protein
(CSBP1) mRNA
M266 D6 H-L35545 C/activated protein 26.29 38.OkDa
C receptor,
endothelial
M300 F1 H-L35594 autotaxin 100.76 9l.OkDa
M318 E2 H-L36720 bystin 33.77 29
M305 H2 H-L37127 RNA polymerise II 12.98 16
M300 D1 H-L38490 ADP-ribosylation factor22.22 32
(GB:L38490)
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M318 E1 H-L38941 rib 12.98 18
oso
mal protein L34
C2 H-L38969 _ 105.27 110
_
_
Homo sapiens ~spondin
3
(THBS3) gene, complete
cds
M476 F4 H-L39060 Homo Sapiens transcription49.61 S3.OkDa
factor
SL 1 mRNA, complete
cds
M300 E4 H-L40399 hypothetical protein29.26 36
(GB:L40399)
E3 H-L40802 Homo Sapiens 17-beta-42.68 60
hydroxysteroid dehydrogenase
( 17-HSD) gene
M478 F1 H-L40904 H. Sapiens peroxisome52.69 60.OkDa
proliferator activated
receptor
gamma, complete cds
M306 C2 H-L41268 natural killer associated37.62 40
transcript
2 ~~*]
M306 E2 H-L4I270 natural killer associated50.16 6S.OkDa
transcript
4 [NK.AT4*]
M306 F2 H-L41347 natural killer associated33.55 40
transcript
S [NKATS*]
M468 C3 H-L413S 1 Homo Sapiens prostasin37.84 4S.OkDa
mRNA,
complete cds
169-S3 H-L41816 Homo Sapiens cam 48 40.77
kinase I
mRNA
167-2S H-L41939 tyrosine-protein 108 108.6
kinase receptor
EPH-3 precursor
C3 H-L42374 Homo sapiens protein54.78 64
phosphatase 2A BS6-beta
(PP2A)
mRNA, complete cds
M306 B 1 H-L42S31 glutathione syntltetaseS2.2S S4
OkDa
M302 F6 H-L42856 RNA polymerase II 13.09 .
transcription 20.OkDa
factor SIII, p 18
subunit
M313 C7 H-L76200 guanylate kinase 21.78 32
(GUK 1 ) OkDa
M428 E1 H-L76702 Homo Sapiens protein66.33 .
68.OkDa
phosphatase 2A B56-delta
(PP2A)
mRNA, complete cds
M478 A 1 H-L76703 Homo Sapiens proteinS 1.48 60.OkDa
phosphatase 2A BS6-epsilon
{PP2A) mRNA, complete
cds
166-S2 H-L77213 H.sapiens phosphomevalonate34 21.19
kinase mRNA
169-64 H-L77964 H.sapiens ERIC3 mRNA100 79
38
M360 C3 H-MIOOSO fatty-acid-binding 14.08 .
protein 2, 20.OkDa
intestinal
DS H-MI0050 fatty-acid-binding 14.08 36
protein 2,
intestinal
M421 E7 H-M10058 Asialoglycoprotein 32.12 48
receptor 1 OkDa
M429 D3 H-M10901 Glucocorticoid receptor85.58 .
BS.OkDa
M312 G1 H-MI 1025 asialoglycoprotein 34.32 34
receptor 2 OkDa
167-44 H-M11026 interferon alpha-4 33 .
precursor 20
86
F2 H-MI 1321 Human group-specificS2.2S .
component S6
vitamin D-binding
protein
mRNA, complete cds
M236 BS H-M I 1354 histone H3.2, CENTRAL15.07 24
ROLE
IN NUCLEOSOME
FORMATION.
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M236 G2 H-M11433 retinol-binding protein14.96 28
I, cellular
transport protein
M270 G7 H-MI 1560 aldolase A , FRUCTOSE-40.15 40
BISPHOSPHATE ALDOLASE
A
[Homo sapiens]
H3 H-M 11717 Human heat shock protein70.51 60
(hsp
70) gene, complete
cds
E1 H-M12523 Human serum albumin 67.1 70
(ALB)
gene, complete cds
B5 H-M12963 Alcohol dehydrogenase41.36 48
1 (class
I), alpha polypeptide
D6 H-M13228 51.15 50
D4 H-M13981 Inhibin, alpha 40.37 50
M236 G4 H-M13982 interleukin 4 [IL4] 16.94 30
precursor, B-
cell activator
M271 B6 H-M14043 lipocortin II, Annexin37.4 45.OkDa
II
(lipocortin II). CALCIUM-
REGULATED MEMBRANE_
BINDING PROTEIN
M271 F4 H-M14218 argininosuccinate 51.04 56
lyase
M297 A3 H-M14221 cathepsin B 37.4 32
OkDa
M305 B2 H-M14328 enolase, alpha 47.85 .
50
167-54 H-M 14333 human c-syn protooncogene60 59
14
167-51 H-M14505 H.sapiens mRNA (open 36 .
reading 33.40
frame; patient SK29(AV))
215-74 H-M 14676 human src-like kinase60 59.14
(slk)
mRNA
167-55 H-M 14780 "creative kinase, 52 41
m chain" 98
M416 F8 H-IvI15059 Fc fragment of IgE, 35.42 .
low affinity 45.OkDa
II, receptor for (CD23A)
M271 F 1 H-M 15182 glucuronidase, beta 71.72 72
[GUSB],
PLAYS AN IMPORTANT
ROLE
IN THE DEGRADATION
OF
DERMATAN AND KERATAN
SULFATES.
215-37 H-M15465 human pyruvate kinase64 59.80
type L
mRNA
M298 A4 H-M 15796 cyclin 28.82 43
OkDa
C3 H-M15800 Mal, T-cell differentiation16 .
protein .94 17
M440 EI H-M15841 Human U2 small nuclear_ 34.OkDa
RNA- 24.86
associated B" antigen
mRNA,
complete cds
M248 C3 H-M15887 endozepine 9.68 lS
OkDa
M463 A2 H-M15990 human c-yes-1 mRNA 59.800 .
65
OkDa
M418 E2 H-M16038 tyrosine-protein kinase56.390 .
LYN 64
OkDa
M266 D3 H-M 16342 HETEROGENEOUS NUCLEAR32.01 .
49
RIBONUCLEOPROTEINS
C1/C2 [Homo Sapiens];
small
nuclear ribonucleoprotein,
polypeptide C
167-20 H-M16591 tyrosine-protein kinase60 55
HCK 62
C7 H-M16591 tyrosine-protein kinase55.620 .
HCK 70
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M305 E7 H-M16660 heat shock 90kD protein79.75 80
1, beta
[HSPCB)
167-65 H-M16750 PIM-1 proto-oncogene38 34.50
~
serine/threonine-protein
kinase
M311 A 1 H-M 16827 acyl-Coenzyme A dehydrogenase,46.42 SO.OkDa
C-4 to C-12 straight-chain
D3 H-M16961 Alpha-2-HS-glycoprotein40.48 50
alpha
and beta chain
D3 H-M16974 Complement component64.35 55
8, alpha
polypeptide
M248 C2 H-M 17017 INTERLEUICIN-8 PRECURSOR11 11
[Homo Sapiens)
M305 E4 H-M17885 ribosomal phosphoprotein34.98 37.OkDa
P0,
acidic
M339 E2 H-M17887 ribosomal phosphoprotein12.76 l9.OkDa
P2
M248 DS H-M18731 galactose-1-phosphate41.91 42
uridylyltransferase
[GALT]
F2 H-M19309 Troponin Tl, skeletal,30.69 40
slow
M385 E2 H-M19713 tropomyosin, alpha, 31.35 4l.OkDa
muscle
167-79 H-M19722 proto-oncogene tyrosine-protein64 58.26
kinase FGR
M248 H1 H-M20560 Annexin III (lipocortin35.64 37
III),
INHIBITOR OF
PHOSPHOLIPASE A2
M235 H1 H-M20681 GLUCOSE TRANSPORTER 54.67 50
TYPE 3, BRAIN
167-29 H-M21616 beta platelet-derived121 121.7
growth
factor receptor precursor
M305 A3 H-M21812 myosin light chain 18.81 30
2
167-30 H-M22146 "405 ribosomal protein34 26.91
S4, x
isofonn"
M302 D6 H-M22430 phospholipase A2 15.95 3l.OkDa
RASF-A
E2 H-M22491 Bone morphogenetic 52.03 55
protein 3
(osteogenic)
M340 A2 H-M22538 NADH-ubiquinone reductase,27.5 33
24
kDa subunit, mitochondria)
B2 H-M22632 Glutamic-oxaloacetic47.41 47
transaminase 2, mitochondria)
(aspartate aminotransferase
2)
B4 H-M22960 Protective protein 52.91 60
for beta-
galactosidase (galactosialidosis)
M250 C4 H-M22995 ras-related protein
RAP1A,
member of RAS oncogene
family
B3 H-M23254 Calpain, large polypeptide77.1 77
L2 I
M266 B4 H-M23613 Nucleophosmin (nucleolar32.45 42
phosphoprotein B23,
numatrin),
BELIEVED TO BIND
SINGLE-
STRANDED NUCLEIC
ACIDS
M469 D2 H-M23668 Homo Sapiens adrenodoxin20.35 25
gene OkDa
M478 H3 H-M24439 Human liver/bone/kidney-type57.75 .
64.OkDa
alkaline phosphatase
(ALPL)
gene
FS H-M24470 Glucose-6-phosphate 38.06 44
dehydrogenase
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M270 ES H-M24898 thyroid hormone triiodothyronine67.65 85
receptor c-erbA,
ear-1, Thyroid
hormone receptor,
alpha (avian
erythroblastic leukemia
viral (v-
erb-a) oncogene homology
D3 H-M24902 Acid phosphatase, 42.57 S4
prostateprostate
D6 H-M2S809 ATPase, H+ transport56.32 57
iysosomal (vacuolar
proton
pump), beta polypeptide,
S6/58kD, isofonm
1
167-77 H-M262S2 "pyruvate kinase, 60 58
M2 isozyme" 48
M271 F8 H-M26326 keratin 18 47.41 .
S0
0kDa
B 1 H-M26901 Human renin gene 44.44 .
SO
M271 G4 H-M27396 asparagine synthetase61.82 62
M338 B3 H-M27S42 globulin, sex hormone-binding39.200 40
MS 12 B6 H-M27602 Protease, serine, 27.28 36
2 (trypsin 2) OkDa
M270 B6 H-M27691 DNA-binding protein 36.08 .
CREB, SO
CAMP-responsive
C 1 H-M27878 Zinc forger protein 8129 81
84 (HPF2)
M270 F6 H-M28209 guanine nucleotide-binding22.66 30.OkDa
protein rabl
MS 12 HS H-M28210 RAB3A, member RAS 24.31 36.OkDa
oncogene
family
B3 H-M28214 Homo sapiens GTP-binding24.2 34
protein (RAB3B) mRNA,
complete cds
M300 CS H-M28249 integrin, alpha 2 130.02 130.OkDa
(CD49B, alpha 2
subunit of VLA-2
receptor)
[ITGA2]
M248 B6 H-M28372 zinc forger protein 19.58 28.OkDa
9 (a cellular
retroviral nucleic
acid binding
protein) [ZNF9]
M248 CS H-M28983 interleukin 1, alpha29.92 42
[IL 1 A]
M298 Cl H-M29S36 translation initiation36.74 SO.OkDa
factor 2, beta
subunit
M42S AS H-M29696 Interleukin 7 receptor50.6 63
OkDa
E1 H-M29960 Human steroid receptor66.44 .
(Tlt2-11) 6S
mltNA, complete cds
M361 D3 H-M29971 6-O-methylguanine-DNA22.88 33.OkDa
methyltransferase
[MGMT]
167-67 H-M30448 "casein kinase II, 34 23
beta chain" 72
M2S0 E2 H-M31211 MYOSIN LIGHT CHAIN 22.99 .
1, 30.OkDa
SLOW-TWITCH MUSCLE
A
ISOFOItM jHomo sapiens]
M311 C4 H-M314S2 proline-rich protein65.78 68
M312 H3 H-M31469 ras-like protein 23.87 32
TC4 OkDa
167-41 H-M31606 "phosphorylase B SO .
kinase gamma 44,7
catalytic chain,
testis isoform"
B4 H-M31642 Hypoxanthine 24.09 36
phosphoribosyltransferase
1
( Lesch-Nyhan syndrome)
M416 D8 H-M31932 Fc fragment of IgG, 34.98 4S.OkDa
low affinity
I Ia, receptor for
(CD32)
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M305 A8 H-M32011 neutrophil cytosolic57.97 58
factor 2
(65kD, chronic granulomatous
disease, autosomal
2) [NCF2]
B2 H-M32315 Human tumor necrosis50.82 60
factor
receptor mRNA, complete
cds
M266 C2 H-M33374 cell adhesion protein14.96 lB
SQM1 OkDa
M431 F1 H-M33375 dihydrodiol dehydrogenase33.99 .
4 40
OkDa
G6 H-M33680 Human 26-kDa cell 26.07 .
surface 24
protein TAPA-1 mRNA,
complete
cds
F1 H-M33772 Human fast skeletal 17.71 29
muscle
troponin C gene
167-15 H-M34065 m-phase induc 55 52
er phosphatase 3 10
F4 H-M34079 _ 44.55 .
Human immunodeficiency 52
vuus
tat transactivator
binding protein-
1 (tbp-I) mRNA, complete
cds
169-86 H-M34181 "CAMP-dependent protein50 38.68
kinase,
beta-catalytic subunit"
D1 H-M34379 Elastatse 2, neutrophil29.48 35
M314 E1 H-M34671 CD59 glycoprotein 14.150 20
precursor
M266 C3 H-M35252 CO-029 (GB:M35252) 26.18 30
M315 A4 H-IvI36035 benzodiazapine receptor18.7 19
(peripheral) [BZRP]
M300 C 1 H-M36340 ADP-ribosylation 20.02 30
factor 1
M312 C3 H-M36341 ADP-ribosylation 19.91 29
factor 2
D6 H-M36634 Vasoactive intestinal18.81 28
peptide
169-26 H-M36881 proto-oncogene tyrosine-protein60 56.06
kinase LCK
167-76 H-M36981 nucleoside diphosphate26 16
kinase B 79
M298 D6 H-M37400 aspartate aminotransferase,45.54 .
SO.OkDa
cytosolic
167-88 H-M37712 galactosyltransferase55 48.36
associated
protein kinase P58/GTA
M424 F4 H-M38258 Retinoic acid receptor,50.05 58
gamma 1 OkDa
M266 H3 H-M38690 CD9 antigen, INVOLVED25.19 .
IN 26.OkDa
PLATELET ACTIVATION
AND
AGGREGATION.
M270 AS H-M55265 casein kinase II, 43.12 50
alpha catalytic
subunit
169-74 H-M55284 human protein kinase80 75.09
C-L
(PRKCL) mRNA
M512 B3 H-M55514 Potassium voltage-gated71.94 IOO.OkDa
channel,
shaker-related subfamily,
member
4
M271 FS H-M57567 ADP-ribosylation 19.91 32.OkDa
factor S [ARS].
INVOLVED IN PROTEIN
TRAFFICKING AND ACTS
AS
AN ALLOSTERIC ACTIVATOR
OF CHOLERA TOXIN.
M250 DI H-M57627 i nterleukin 10 [IL10],19.69 27
SUPPRESSOR FACTOR
FOR
TH1 IMMUNE RESPONSES
( BY SIMILARITY).
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IvI302 D3 H-M57730 EPH-related receptor22.620 ~ 36.OkDa
tyrosine
kinase ligand 1 precursor
M248 BS H-M58458 ribosomal protein 29.04 36.OkDa
S4, X-linked
[RPS4X]
M248 AS H-M58459 ribosomal protein 29.04 36
S4, Y-linked
[ltPS4Y]
M248 GS H-M58525 CATECHOL O- 29.92 36
METHYLTRANSFERASE,
MEMBRANE-BOUND FORM
[Homo Sapiens], COMT
M482 B2 H-M59916 Sphingomyelin phosphodiesterase69.3 69.OkDa
I, acid lysosomal
(acid
sphingomyelinase)
M390 C1 H-M60091 galactose-1-phosphate41.8 SO.OkDa
uridylyltransferase
M316 B1 H-M60314 bone morphogenetic 50.05 55
protein 5
[BMPS]
B4 H-M60459 Erythropoietin receptor55.99 60
C7 H-M60483 Human protein phosphatase34.1 56
2A
catalytic subunit-alpha
gene,
complete cds
M462 D7 H-M60484 Human protein phosphatase34.1 44.OkDa
2A
catalytic subunit-beta
gene,
complete cds
A 12 H-M60527 deoxycytidine kinase28.b70 50
167-5 H-M60724 human p70 ribosomal 66 57.82
S6 kinase
alpha-I mRNA
167-17 H-M60725 human p70 ribosomal 62 55.29
S6 kinase
alpha-II mRNA
M271 A4 H-M61199 cleavage signal 1, 27.5 36.OkDa
ESTs, Highly
similar to CLEAVAGE
SIGNAL-
1 PROTEIN [Homo sapiens]
B1 H-M61733 Homo Sapiens erythroid70.62 71
membrane protein
4.1 mRNA,
complete cds
M298 A1 H-M61764 tubulin, gamma 49.72 SS.OkDa
M422 E2 H-M62505 Complement component38.61 38.OkDa
5
receptor 1 (CSa ligand)
M313 GS H-M62810 transcription factor27.17 35.OkDa
1,
mitochondrial
C9 H-M62839 apolipoprotein H 38.06 60
GS H-M63154 Gastric intrinsic 45.98 52
factor (vitamin
B
synthesis)
167-6 H-M63167 RAC-alpha serine/threonine64 52.87
kinase
B 1 H-M63573 Pepddylprolyl isomerase23.87 33
B
(cyclophilin B)
M302 H2 H-M63603 phospholamban 5.83 6
M306 D1 H-M63838 interferon, gamma-inducible80.3 108
protein 16
M423 H3 H-M63959 Low density lipoprotein-related39.38 48.OkDa
protein-associated
protein 1
(alpha-2-macroglobulin
receptor-
associated protein
1
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G3 H-M64099 Human gamma-glutmyl 64.57 S2
transpeptidase-related
protein
(GGT-Rel) mRNA, complete
cds
M47S B8 H-M64673 Human heat shock factor58.3 6S.OkDa
1
(TCFS) mRNA, complete
cds
M266 DS H-M64716 ribosomal protein 13.86 l7.OkDa
S2S
M248 C6 H-M647S2 glutamate receptor, 99.88 100
ionotropic,
AMPA 1 [GRIAlJ
M312 G3 H-M6492S pahnitoylated membraneS L37 S 1.OkDa
protein,
erythrocyte, SS kDa
M302 C7 H-M6S292 complement factor 36.41 SO
H-related
protein (GB:M6S292)
D3 H-M68S 16 Human protein C inhibitor44.77 S4
gene,
complete cds
167-27 H-M68S20 cell division protein38 32.85
kinase 2
M236 DS H-M68867 Cellular retinoic 15.29 l9.OkDa
acid-binding
protein 2, MAY REGULATE
THE ACCESS OF RETINOIC
ACID TO THE NUCLEAR
RETINOIC ACID RECEPTORS.
M441 E1 H-M69226 monoamine oxidase 58.08 64.OkDa
A [MAOA]
M298 DS H-M72393 calcium-dependent 82.5 117.OkDa
phospholipid-
binding protein [PLA2"']
M422 DS H-M73238 Ciliary neurotrophic 41.03 SI.OkDa
factor
receptor
C1 H-M732SS Human vascular cell 81.4 81
adhesion
molecule-1 (VCAM1)
gene,
complete CDS
M422 G6 H-M73481 Human gastrin releasing42.35 4S.OkDa
peptide
receptor (GRPR) mRNA,
complete cds
M23S G6 H-M73499 carboxylesterase, 62.48 90.OkDa
INVOLVED IN
THE DETOXIFICATION
OF
XENOBIOTICS AND THE
ACTIVATION OF ESTER
AND
AMIDE PRODRUGS.
M302 D1 H-M73S47 polyposis locus DP1 20.46 28
M300 H4 H-M73969 interleukin 8 receptor,39.71 36
beta
[IL8RB]
G 1 H-M74491 ADP-ribosylation factor20.02 31
3
B4 H-M74816 49.5 SO
B2 H-M7S 110 H,K-ATPase, beta subunit32.12 37
M416 B8 H-M76766 General transcription34.87 44.OkDa
factor IIB
167-18 H-M77198 RAC-beta serine/threonine64 57.27
kinase
167-87 H-M77348 PMEL 17 protein precursor74 73.SS
C4 H-M77698 YY1 transcription 4S.6S 48
factor
M248 G6 H-M80261 apurinic/apyrimidinic35.09 37.OkDa
(abasic)
endonuclease [APE],
REPAIRS
OXIDATIVE DNA DAMAGES
IN VITRO
169-SO H-M803S9 putative serine%threonine-protein80 78.50
kinase P78
M330 H1 H-M80461 immunoglobulin-associated25.370 27.OkDa
beta
(B29) [IGBJ
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169-I H-M8061_3 ring3 protein 100 83.01
"
M298 A2 H-M80783 B12 protein 34.87 43.OkDa
217-1 H-M81457 calpactin I light 10 10.74
chain
M422 C6 H-M81589 Homo Sapiens serotonin41.58 4l.OkDa
ID
receptor (5-HT 1
D' ) mRNA,
complete cds
M424 A1 H-M81590 Homo sapiens serotonin43.01 48.OkDa
ID
receptor (5-HT1D~)
mRNA,
complete cds
M250 Hl H-M81592 gamma-glutamyl carboxylase83.49 85
[GGCX], CONVERTS
GLUTAMATE RESIDUES
TO
GAMMA-
CARBOXYGLUTAMATE
M250 F2 H-M81601 TRANSCRIPTION 33.22 36.OkDa
ELONGATION FACTOR
S-II
[Homo sapiens]
C2 H-M81650 Human semenogelin 50.93 52
I (SEMGI)
gene, complete cds
M266 A4 H-M81757 ribosomal protein 16.06 18
S19
169-61 H-M81933 m-phase inducer phosphatase57 57.60
1
M302 HI H-M82809 annexin IV 35.42 38.OkDa
M300 C4 H-M83653 cytoplasmic phosphotyrosyl17.49 28.OkDa
protein phosphatase,
type 1
169-14 H-M83941 tyrosne-protein kinase108 108.2
receptor
ETK I precursor
F1 H-M84443 Galactokinase 2 50.49 52
M305 H6 H-M84747 interleukin 9 receptor57.53 58
[IL9R]
167-53 H-M86400 14-3-3 protein zeta/delta33 27.02
M271 C8 H-M86521 transketolase 68.64 68.OkDa
169-51 H-M86699 human kinase (TTK) 92 92.58
mRNA
M316 F2 H-M86752 transformation-sensitive59.84 60.OkDa
protein
M270 C8 H-M86921 membrane glycoprotein24.97 34
mb-1,
Immunoglobulin-associated
alpha, ASSOCIATED
TO
SURFACE IGM-RECEPTOR;
MAY BE INVOLVED IN
SIGNAL TRANSDUCTION
A5 H-M87507 Homo sapien interleukin-144.55 50
beta
convertase (ILIBCE)
mRNA,
complete cds
M305 B7 H-M88011 glucokinase [GCK] 51.26 60
M305 H1 H-M88279 immunophilin FKBP52 50.6 64.OkDa
M420 F1 H-M88468 mevalonate kinase 43.600 47.OkDa
M305 A7 H-M89913 dUTP pyrophosphatase15.62 19
(dUTPase) [DUT*]
M316 E2 H-M90657 tumor-associated 22.33 28
antigen L6
167-31 H-M90813 human D-type cyclin 36 31.86
(CCND2)
mRNA
A 1 H-M91036 H.sapiens G-gamma 16.28 18
globin and
A-gamma globin genes,
complete
cds's
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G2 H-M91463 Human glucose transporter55.66 52
(GLUT4) gene, complete
cds
A1 H-M91670 Human ubiquitin carrier24.86 36
protein
(E2-EPF) mRNA, complete
cds
E4 H-M92444 Homo Sapiens 35.09 45
apurinic/apyrimidinic
endonuclease (HAPl)
gene,
complete cds
M305 C4 H-M94556 single-stranded DNA-binding16.39 20
protein, mitochondrial
G 12 H-M94856 fatty-acid-binding 14.96 36
protein
homolog
M453 C3 H-M95623 Homo Sapiens 39.82 SO.OIcDa
hydroxymethylbilane
synthase
gene, complete cds
M302 F2 H-M95787 smooth muscle protein22.22 33.OkDa
SM22
AI H-M95809 Human basic transcription60.39 64
factor
621cD subunit (BTF2),
complete
cds
M271 E8 H-M96982 small nuclear ribonucleoprotein26.51 39.OIcDa
U2 auxiliary factor,
35 kDa ,
SPLICING FACTOR U2AF
35
KD SUBUNIT. NECESSARY
FOR THE SPLICING OF
PRE-
mRNA.
M416 B3 H-M96995 Growth factor receptor-bound23.98 32.O1cDa
protein 2
G2 H-M96995 Growth factor receptor-bound23.98 49
protein 2
H4 H-M97016 Bone morphogenetic 44.33 61
protein 8
(osteogenic protein
2)
M271 DI H-M97190 Sp2 transcription 54.56 60
factor [SP2],
BINDS TO GC BOX
PROMOTERS ELEMENTS
AND
SELECTIVELY ACTIVATES
mRNA SYNTHESIS FROM
GENES THAT CONTAIN
FUNCTIONAL RECOGNITION
SITES.
M271 C1 H-M97191 Sp3 transcription 71.94 72
factor [SP3],
BINDS TO GT AND GC
BOXES
PROMOTERS ELEMENTS.
PROBABLE
TRANSCTRIPTIONAL
ACTIVATOR.
M305 C7 H-M97388 transcription repressor19.47 30
(interacting
with the TATA-binding
protein)
[DRI *]
217-13 H-M97675 human transmembrane 100 103.1
receptor
(rorl) mRNA
B3 H-M97856 Nuclear autoantigenic86.68 87
sperm
protein (histone-binding)
M429 G2 H-M97935 Homo Sapiens transcription82.61 89.O1cDa
factor
ISGF-3 mRNA, complete
cds
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D1 H-M99487 Human prostate-specific82.61 92
membrane antigen (PSM)
mRNA,
complete cds
M363 A1 H-P0002 riboflavin synthase 17.27
beta chain
(ribE)
M363 B 1 H-P0004 carbonic anhydrase 24.42
(icfA)
M363 C1 H-P0005 orotidine 5'-phosphate25.08
decarboxylase (pyrF)
M363 D1 H-P0006 pantoate-beta-alanine30.47
ligase
(PAC)
M379 A1 H-P0010-2 chaperone and heat 60.17
shock protein
(groEL)
M363 E1 H-P0011 co-chaperone (groES) 13.09
M363 F1 H-P0012 DNA primase (dnaG) 61.6
M363 GI H-P0013 hypothetical protein 38.61
M363 H1 H-P0014 hypothetical protein 30.36
M363 A2 H-P0015 hypothetical protein 10.34
M363 B2 H-P0016 hypothetical protein 9.68
M363 C2 H-P0017 virB4 homolog (virB4)86.68
M363 D2 H-P0018 hypothetical protein 51.7
M363 E2 H-P0021 hypothetical protein 21.01
M363 F2 H-P0022 conserved hypothetical57.42
integral
membrane protein
M363 G2 H-P0026 citrate synthase (gltA)46.97
M363 H2 H-P0027 isocitrate dehydrogenase46.86
(icd)
M363 A3 H-P0028 conserved hypothetical19.58
secreted
protein
M363 B3 H-P0030 hypothetical protein 65.34
M363 C3 H-P0031 hypothetical protein 15.18
M363 D3 H-P0034 aspartate 1-decarboxylase12.98
(panD)
M363 E3 H-P0035 conserved hypothetical10.78
protein
M363 F3 H-P0037 NADH-ubiquinone 38.72
oxidoreductase subunit
M363 G3 H-P0044 GDP-D-mannose dehydratase42.02
(rfbD)
M363 H3 H-P0047 hydrogenase expression/formation36.63
protein (hypE)
M363 A4 H-P0048 transcriptional regulator84.7
(hypF)
M363 B4 H-P0052 hypothetical protein 36.41
M363 C4 H-P0055 proline permease (putP)54.67
M363 D4 H-P0056 delta-1-pyrroline-5-carboxylate130.46
dehydrogenase
M363 E4 H-P0057 hypothetical protein 7,7
M363 F4 H-P0063 hypothetical protein 54.67
M363 G4 H-P0064 hypothetical protein 15.4
M363 H4 H-P0066 conserved hypothetical91.52
ATP-
binding protein
M363 AS H-P0067 urease accessory protein29.26
(ureH)
M363 BS H-P0068 urease accessory protein22
(ureG)
M363 CS H-P0075 urease protein (ureC)49.06
M363 DS H-P0077 peptide chain release38.83
factor RF-1
(PEA)
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M363 ES H-P0082 methyl-accepting chemotaxis74.14
transducer (tlpC)
M363 FS H-P0086 conserved hypothetical49.61
protein
M363 G5 H-P0087 hypothetical protein 50.38
M363 HS H-P0088 RNA polymerise sigma-7073.92
factor
(rpoD)
M363 A6 H-P0089 pfs protein (pfs) 25.52
M363 B6 H-P0090 malonyl coenzyme A-acyl34.1
carrier
protein transacylase
(fibD)
M363 C6 H-P0093 hypothetical protein 12.21
M363 D6 H-P0096 phosphoglycerate dehydrogenase34.65
M304 A1 H-P0099 methyl-accepting chemotaxis74.36
protein (tlpA)
M304 B1 H-PO100 conserved hypothetical40.59
protein
M304 C 1 H-P0101 hypothetical protein 27.94
M304 D1 H-P0104 2',3'-cyclic-nucleotide64.02
2'-
phosphodiesterase
(cpdB)
M304 E 1 H-P0105 conserved hypothetical17.16
protein
M304 F1 H-P0106 cystathionine gamma-synthase41.91
(metB)
M304 GI H-P0107 cysteine synthetase 33.77
(cysK)
M304 H1 H-P0108 hypothetical protein 20.57
M304 A2 H-P0109 chaperone and heat 68.31
shock protein
70 (dnaK)
M304 B2 H-P0110 co-chaperone and heat20.9
shock
protein (grpE)
M304 C2 H-PO111 hypothetical protein 30.47
M304 D2 H-P0113 hypothetical protein 10.89
M304 E2 H-P0114 hypothetical protein 69.19
M304 F2 H-PO115 flagellin B (flag) 56.65
M304 G2 H-P0116 DNA topoisomerase 81.07
I (topA)
M304 H2 H-P0117 conserved hypothetical33.99
protein
M304 A3 H-P0118 hypothetical protein 43.56
M304 B3 H-P0119 hypothetical protein 50.82
M304 C3 H-P0120 hypothetical protein 43.89
M304 D3 H-P0121 phosphoenolpyruvate 89.43
synthase
(PPS)
M304 E3 H-P0122 hypothetical protein 4.84
M304 F3 H-P0123 threonyl-tRNA synthetase67.43
(thrS)
M304 G3 H-P0124 translation initiation22.44
factor IF-3
(intC)
M304 H3 H-P0125 ribosomal protein 7.15
L35 (rp135)
M304 A4 H-P0126 ribosomal protein 12.87
L20 (rp120)
M304 B4 H-P0127 outer membrane protein31.57
(omp4)
M304 C4 H-P0128 hypothetical protein 4.62
M304 D4 H-P0129 hypothetical protein 15.62
M304 E4 H-P0130 hypothetical protein 31.57
M304 F4 H-P0131 hypothetical protein 3.74
M304 G4 H-P0132 L-serine deaminase 50.16
(sdaA)
M304 H4 H-P0133 serine transporter 45.54
(sdaC)
M304 AS H-P0134 3-deoxy-D-arabino-heptulosonate49.5
7-phosphate synthase
(dhs 1 )
M304 BS H-P0135 hypothetical protein 4.95
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M304 CS H-P0136 bacterioferritin comigratory16.83
protein (bcp)
M304 DS H-)?0137 hypothetical protein 23.32
M304 ES H-P0138 conserved hypothetical53.02
iron-sulfur
protein
M304 FS H-P0139 conserved hypothetical26.73
secreted
protein
M304 GS H-P0140 L-lactate permease 60.5
(IctP)
M304 HS H-P0141 L-lactate permease 60.72
(IctP)
M304 A6 H-P0142 A1G-specific adenine 36.19
glycosylase
(mutt)
M304 B6 H-P0144 cytochrome c oxidase,53.79
heme b
and copper-binding
subunit,
membrane-bound (fixN)
M304 C6 H-P0145 cytochrome c oxidase,25.63
monoheme
subunit, membrane-bound
(fix0)
M304 D6 H-P0146 cbb3-type cytochrome 8.14
c oxidase
subunit Q (CcoQ)
M304 E6 H-P0147 cytochrome c oxidase,31.57
diheme
subunit, membrane-bound
(fucP)
M304 F6 H-P0148 hypothetical protein 7.59
M304 G6 H-PO150 hypothetical protein 21.67
M304 H6 H-P0152 hypothetical protein 31.68
M304 A7 H-P0153 recombinase (recA) 38.28
M304 B7 H-P0154 enolase (eno) 46.97
M304 C7 H-PO155 hypothetical protein 10.12
M304 D7 H-P0157 shikimic acid kinase 17.93
I {aroK)
M304 E7 H-P0158 hypothetical protein 35.09
M304 F7 H-P0159 lipopolysaccharide 41.03
1,2-
glucosyltransferase
(rfa~
M304 G7 H-P0161 hypothetical protein 4.07
M304 H7 H-P0162 conserved hypothetical26.51
protein
M304 A8 H-P0163 delta-aminolevulinic 35.64
acid
dehydratase (hemB)
M304 B8 H-P0164 signal-transducing 28.05
protein,
histidine kinase
M304 C8 H-P0165 hypothetical protein 19.14
M304 D8 H-P0166 response regulator 24.86
(ompR)
M304 E8 H-P0167 hypothetical protein 17.38
M304 F8 H-P0168 hypothetical protein 9.68
M304 G8 H-P0170 hypothetical protein 27.94
M304 H8 H-P0171 peptide chain release40.04
factor RF-2
(P~)
M304 A9 H-P0172 molybdopterin biosynthesis43.12
protein (moeA)
M304 B9 H-P0173 tlagellar biosynthetic28.16
protein
(fliR)
M304 C9 H-P0174 hypothetical protein 28.49
M304 D9 H-P0175 cell binding factor 33
2
M304 E9 H-P0176 fructose-bisphosphate33.88
aldolase
{tsr)
M304 F9 H-P0177 translation elongation20.68
factor EF-P
(efp)
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M304 G9 H-P0178 spore coat polysaccharide37.51
biosynthesis protein
E
M304 H9 H-P0179 ABC transporter, ATP-binding23.54
protein
M304 A10 H-P0180 apolipoprotein N-acyltransferase46.86
(cute)
M304 B10 H-P0182 lysyl-tRNA synthetase55.22
(lysS)
M304 C10 H-P0183 serine hydroxymethyltransferase45.87
(glyA)
M304 D10 H-P0184 hypothetical protein 19.91
M304 E10 H-P0185 hypothetical protein 29.48
M304 F10 H-P0186 hypothetical protein 44.55
M304 G10 H-P0187 hypothetical protein 10.56
M304 H10 H-P0188 hypothetical protein 3.74
M304 A11 H-P0189 conserved hypothetical19.58
integral
membrane protein
M304 BI1 H-P0190 conserved hypothetical55.33
secreted
protein
M304 C11 H-P0191 fumarate reductase, 27.06
iron-sulfur
subunit (frdB)
M304 Dl H-P0192 fumarate reductase, 78.65
l flavoprotein
subunit (frdA)
M304 E11 H-P0193 fumarate reductase, 28.16
cytochrome b
subunit (frdC)
M304 Fl H-P0194 triosephosphate isomerase25.85
l (tpi)
M304 G11 H-P0195 enoyl-(acyl-Garner-protein)30.36
reductase (NADH) (fabl)
M365 A1 H-P0197 S-adenosyhnethionine 42.46
synthetase
2 (metX)
M365 B1 H-P0203 hypothetical protein 10.12
M365 C1 H-P0209 hypothetical protein 49.61
M365 D1 H-P02I3 glucose inhibited 68.42
division protein
(gig)
M381 E1 H-P0218 hypothetical protein 20.24
M365 E1 H-P0221 nifU-like protein 35.97
M365 F1 H-P0227 outer membrane protein76.12
(omp5)
M365 G1 H-P0228 conserved hypothetical43.01
integral
membrane protein
M365 H1 H-P0230 CTP:CMP-3-deoxy-D-manno-26.84
octulosonate-cytidylyl-transferase
(kdsB)
M365 A2 H-P0233 conserved hypothetical43.01
protein
M365 B2 H-P0235 conserved hypothetical39.16
secreted
protein
M365 C2 H-P0236 hypothetical protein 13.64
M365 D2 H-P0238 prolyl-tltNA synthetase63.58
(pros)
M381 E2 H-P0243 neutrophil activating15.95
protein
(napA) (bacteriofen
itin)
M365 E2 H-P0244 signal-transducing 42.02
protein,
histidine kinase (atoS)
M365 F2 H-P0246 flagellar basal-body 37.73
P-ring protein
(flgI)
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M365 G2 H-P0247 ATP-dependent RNA ~ 54.23
helicase,
DEAD-box family (deaD)
M365 H2 H-P0248 conserved hypothetical39.93
protein
M379 B1 H-P0249-2 hypothetical protein 19.8
M379 C1 H-P0250-2 oligopeptide ABC transporter,56.87
ATP-binding protein
(oppD)
M381 A3 H-P0251 oligopeptide ABC transporter,37.29
permease protein (oppC)
M379 E1 H-P0252-2 outer membrane protein53.68
(omp7)
M365 A3 H-P0254 outer membrane protein47.52
(omp8)
M365 B3 H-P0255 adenylosuccinate synthetase45.32
(purA)
M365 C3 H-P0257 conserved hypothetical24.2
secreted
protein
M365 D3 H-P0259 exonuclease VII, large46.31
subunit
(xseA)
M381 D3 H-P0260 adenine specific DNA 42.35
methyltransferase
(mod)
M365 E3 H-P0263 adenine specific DNA 27.83
methyltransferase
(hpaim)
M365 F3 H-P0264 ATP-dependent protease94.27
binding
subunit (clpB)
M365 G3 H-P0266 dihydroorotase (pyrC)41.69
M365 H3 H-P0267 chlorohydrolase 45.1
M365 A4 H-P0271 hypothetical protein 36.08
M365 B4 H-P0275 ATP-dependent nuclease47.41
(addB)
M381 G3 H-P0276 hypothetical protein 20.46
M365 C4 H-P0278 guanosine pentaphosphate53.35
phosphohydrolase (gppA)
M365 D4 H-P0279 lipopolysaccharide 37.51
heptosyltransferase-1
(rfaC)
M365 E4 H-P0280 heat shock protein 36.19
B (ibpB)
M365 F4 H-P0282 hypothetical protein 52.91
M365 G4 H-P0283 3-dehydroquinate synthase37.84
(aroB)
M365 H4 H-P0284 conserved hypothetical57.64
integral
membrane protein
M365 AS H-P0285 conserved hypothetical46.09
protein
M381 A4 H-P0287 hypothetical protein 19.03
M381 C4 H-P0288 hypothetical protein 17.38
M366 A1 H-P0389 superoxide dismutase 23.54
(soda)
M366 B1 H-P0390 adhesin-thiol peroxidase18.37
(tagD)
M366 C1 H-P0391 purine-binding chemotaxis18.26
protein (cheVl~
M366 D1 H-P0392 histidine kinase (cheA)88.44
M366 E1 H-P0393 chemotaxis protein 34.32
(cheV)
M366 F1 H-P0394 hypothetical protein 27.83
M366 G1 H-P0395 conserved hypothetical24.53
protein
M366 H 1 H-P0396 conserved hypothetical67.87
protein
M366 A2 H-P0397 phosphoglycerate dehydrogenase57.75
(serA)
M366 B2 H-P0398 hypothetical protein 20.13
M366 C2 H-P0399 ribosomal protein 61.27
S 1 (rps 1 )
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M366 D2 H-P0403 phenylalanyl-tRNA 36.19
synthetase,
alpha subunit (pheS)
_
M366 E2 H-P0404 protein kinase C inhibitor11.55
(SP:P16436)
M366 F2 H-P0405 nifS-like protein 48.51
M366 G2 H-P0406 hypothetical protein 21.67
M366 H2 H-P0407 biotin sulfoxide reductase87.67
(bisC)
M381 DI H-P0409 GMP synthase (guaA) 55.99
M381 F1 H-P0410 putative neuraminyllactose-27.5
binding hemagglutinin
homolog
(hp~)
M366 A3 H-P0411 hypothetical protein 11.66
M366 B3 H-P0412 hypothetical protein 3.63
M366 C3 H-P0413 transposase-like protein,29.59
PS3IS
M366 D3 H-P0414 IS200 insertion sequence15.29
from
SARA 17
M366 E3 H-P0415 conserved hypothetical68.64
integral
membrane protein
M366 F3 H-P0416 cyclopropane fatty 42.9
acid synthase
(cfa)
M366 G3 H-P0417 methionyl-tRNA synthetase71.61
(metS)
M366 H3 H-P0418 hypothetical protein 36.96
M366 A4 H-P0419 conserved hypothetical28.82
protein
M366 B4 H-P0420 hypothetical protein 15.73
M366 C4 H-P0421 type 1 capsular polysaccharide42.9
biosynthesis protein
J (capJ)
M366 D4 H-P0422 arginine decarboxylase67.76
(speA)
M366 E4 H-P0424 hypothetical protein 68.2
M366 F4 H-P0425 hypothetical protein 45.98
M366 G4 H-P0427 hypothetical protein 12.32
M366 H4 H-P0433 hypothetical protein 16.28
M366 AS H-P0436 hypothetical protein 13.42
M366 BS H-P0437 IS605 transposase 15.73
(tnpA)
M366 CS H-P0438 IS605 transposase 47.08
(tnpB)
M366 DS H-P0442 hypothetical protein 9.79
M366 ES H-P0445 hypothetical protein 6.82
M366 FS H-P0452 hypothetical protein 57.09
M366 GS H-P0455 hypothetical protein 11.44
M366 HS H-P0457 hypothetical protein 9.68
M366 A6 H-P0463 type I restriction 53.68
enzyme M
protein (hsdM)
M366 B6 H-P0464 type I restriction 116.16
enzyme R
protein (hsdR)
M366 C6 H-P0465 conserved hypothetical69.52
protein
M366 D6 H-P0466 conserved hypothetical28.16
protein
M366 E6 H-P0467 conserved hypothetical12.76
integral
membrane protein
M366 F6 H-P0468 conserved hypothetical54.56
protein
M366 G6 H-P0469 conserved hypothetical17.93
protein
M366 H6 H-P0471 glutathione-regulated45.87
potassium-
efflux system protein
(kefB)
M366 A7 H-P0472 outer membrane protein20.57
(ompl l)
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M366 B7 H-P0473 molybdenum ABC transporter,27.17
periplasmic molybdate-binding
protein (modA)
M366 C7 H-P0474 molybdenum ABC transporter,24.75
permease protein (modB)
M366 D7 H-P0475 molybdenum ABC transporter,29.26
ATP-binding protein
(modD)
M366 E7 H-P0476 glutamyl-tRNA synthetase51.04
{gltX)
M366 F7 H-P0477 outer membrane protein40.48
(ompl2)
M366 G7 H-P0478 adenine specific DNA 60.06
methyltransferase
(VSPIM)
M366 H7 H-P0479 hypothetical protein 31.13
M366 A8 H-P0481 adenine specific DNA 23.32
methyltransferase
(MFOKI)
M366 B8 H-P0482 hypothetical protein 18.81
M366 C8 H-P0483 cytosine specific 36.3
DNA
methyltransferase
(H-PHIMC)
M367 A1 H-P0486 hypothetical protein 58.19
M367 B1 H-P0487 hypothetical protein 52.91
M367 C1 H-P0489 hypothetical protein 32.56
M367 DI H-P0490 putative potassium 41.69
channel
protein, putative
M367 El H-P0491 ribosomal protein 6.93
L28 {rpL28)
M367 F1 H-P0492 hypothetical protein 30.69
M367 G1 H-P0494 UDP-N-acetylmuramoylalanine-46.53
D-glutamate ligase
(murD)
M367 H1 H-P0495 hypothetical protein 9.57
M367 A2 H-P0496 conserved hypothetical14.74
protein
M367 B2 H-P0498 sodium- and chloride-dependent48.73
transporter
M367 C2 H-P0499 phospholipase A1 precursor39.16
(DR-
phospholipase A)
M367 D2 H-PO500 DNA polymerase III 41.25
beta-subunit
(dnaN)
M367 E2 H-PO501 DNA gyrase, sub B 85.14
{gyrB)
M367 F2 H-P0503 hypothetical protein 27.17
M367 G2 H-P0504 hypothetical protein 5.5
M367 H2 H-PO505 hypothetical protein 17.05
M367 A3 H-P0507 conserved hypothetical23.43
protein
M367 B3 H-P0509 glycolate oxidase 50.6
subunit (glcD)
M367 C3 H-POS 10 dihydrodipicolinate 28.05
reductase
{APB)
M367 D3 H-P0512 glutamine synthetase 53.02
(glnA)
M367 E3 H-P0514 ribosomal protein 16.61
L9 (rpl9)
M367 F3 H-PO515 heat shock protein 19.91
(hslV)
M367 G3 H-P0516 heat shock protein 48.84
(hslU) ORF1
M367 H3 H-P0517 GTP-binding protein 33.33
(era)
M367 A4 H-P0519 conserved hypothetical30.47
protein
M367 B4 H-P0520 cag pathogenicity 12.76
island protein
(cagl)
M367 C4 H-P0522 cag pathogenicity 53.02
island protein
(cag3)
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M367 D4 H-P0523 cag pathogenicity 18.7
island protein
(~B4)
M36 7 viral l homolog 36.41
E4 H-P0525
M367 F4 H-P0526 cag pathogenicity 22
island protein
(cag6)
M367 G4 H-P0528 cag pathogenicity 57.53
island protein
(cag8)
M379 H1 H-P0531-2 cag pathogenicity 24.09
island protein
(cagll)
M367 H4 H-P0532 cag pathogenicity 30.91
island protein
(cagl2)
M367 A5 H-P0534 cag pathogenicity 21.67
island protein
(cagl3)
M367 B5 H-P0541 cag pathogenicity 40.81
island protein
- (cag20)
M367 C5 H-P0542 cag pathogenicity 15.73
island protein
(cag21)
M367 D5 H-P0545 cag pathogenicity 22.88
island protein
(cag24)
M367 E5 H-P0549 glutamate racemase 28.16
(glr)
M367 F5 H-P0550 transcription termination48.29
factor
)tho (rho)
M367 G5 H-P0551 ribosomal protein 7.48
L31 (rp131)
M367 H5 H-P0552 conserved hypothetical31.68
protein
M36? A6 H-P0553 conserved hypothetical25.08
protein
M367 B6 H-P0554 hypothetical protein 35.42
M367 C6 H-P0555 hypothetical protein 30.14
M367 D6 H-P0556 hypothetical protein 16.06
M367 E6 H-P0557 acetyl-coenzyme A 34.43
carboxylase
(accA)
M367 F6 H-P0558 beta ketoacyl-acyl 45.43
carrier protein
synthase II (fabF)
M367 G6 H-P0561 3-ketoacyl-acyl carrier27.28
protein
reductase (fabG)
M367 H6 H-P0562 ribosomal protein 7.81
S21 (rps21)
M367 A7 H-P0563 hypothetical protein 45.87
M367 B7 H-P0566 diaminopimelate epimerase30.14
(~PF)
M367 C7 H-P0568 hypothetical protein 28.16
M367 D7 H-P0570 aminopeptidase a/i 54.67
(pepA)
M367 E7 H-P0571 conserved hypothetical21.23
integral
membrane protein
M379 A2 H-P0572-2 adenine 19.8
phosphoribosyltransferase
(apt)
M379 B2 H-P0573-2 hypothetical protein 12.21
M379 C2 H-P0574-2 galactosidase acetyltransferase16.72
(lacA)
M379 D2 H-P0575-2 conserved hypothetical25.63
membrane
protein
M379 E2 H-P0576-2 signal peptidase I 32.01
(IepB)
M367 F7 H-P0577 methylene-tetrahydrofolate32.23
dehydrogenase(folD)
M367 G7 H-P0579 hypothetical protein 20.35
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M367 H7 H-P0580 hypothetical protein 41.03
M367 A8 H-P0581 dihydroorotase (pyrC)37.4
M367 B8 H-P0582 hypothetical protein 35.75
M367 C8 H-P0583 hypothetical protein 32.34
M368 A1 H-P0584 flagellar switch protein13.64
(fliN)
M368 B H-P0585 endonuclease III (nth)24.09
1
M368 C1 H-P0587 aminodeoxychorismate 36.3
lyase
(pabC)
M368 D1 H-P0591 ferredoxin oxidoreductase,20.57
gamma subunit
M368 E1 H-P0593 adenine specific DNA 65.89
methyltransferase
(mod)
M368 F1 H-P0594 hypothetical protein 6.05
M368 GI H-P0596 hypothetical protein 21.23
M368 H1 H-P0597 penicillin-binding 72.6
protein lA
(PBP-1 A)
M368 A2 H-P0599 hemolysin secretion 47.74
protein
precursor(hylB)
M368 B2 H-P0601 flagellin A (flaA) 56.21
M368 C2 H-P0602 endonuclease III 24.09
M368 D2 H-P0603 hypothetical protein 20.9
M379 F2 H-P0608-2 hypotheticai protein 17.71
M368 E2 H-P0614 hypothetical protein 12.32
M368 F2 H-P0616 chemotaxis protein 34.54
(cheV)
M368 G2 H-P0617 aspartyl-tltNA synthetase63.58
(asps)
M368 H2 H-P0621 DNA mismatch repair 83.93
protein
(MutS)
M368 A3 H-P0622 hypothetical protein 13.31
M368 B3 H-P0623 UDP-N-acetylmuramate-alanine49.5
ligase (murC)
M368 C3 H-P0625 protein E (gcpE) 39.6
M368 D3 H-P0626 tetrahydrodipicolinate44.22
N-
succinyltransferase
(dapD)
M368 E3 H-P0627 hypothetical protein 12.21
M368 F3 H-P0629 hypothetical protein 75.02
M368 G3 H-P0630 modulator of drug 21.45
activity
(mda66)
M368 H3 H-P0631 quinone-reactive Ni/Fe42.35
hydrogenase, small
subunit
(hY~)
M368 A4 H-P0632 quinone-reactive Ni/Fe63.69
hydrogenase, large
subunit
(hydB)
M368 B4 H-P0633 quinone-reactive Ni/Fe24.75
hydrogenase, cytochrome
b
subunit (hydC)
M368 C4 H-P0634 quinone-reactive Ni/Fe19.69
hydrogenase (hydD)
M368 D4 H-P0635 hypothetical protein 56.43
M368 E4 H-P0636 hypothetical protein 10.23
M368 F4 H-P0637 hypothetical protein 16.61
M368 G4 H-P0638 outer membrane protein33.66
(ompl3)
M368 H4 H-P0643 glutamyl-tltNA synthetase48.4
(gltX)
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M368 AS H-P0644 conserved hypothetical10.78
integral
membrane protein
M368 BS H-P0645 soluble lytic murein 61.71
transglycosylase (slt)
M368 CS H-P0646 UDP-glucose pyrophosphorylase30.14
(galU)
M368 D5 H-P0647 hypothetical protein 14.96
M368 ES H-P0648 UDl'-N-acetylglucosamine46.53
enolpyruvyl transferase
(murZ)
M368 FS H-P0649 aspartate ammonia-lyase51.59
(aspA)
M368 GS H-P0650 hypothetical protein 21.67
M379 A3 H-P0651-2 fucosyltransferase 52.47
M381 E3 H-P0652 phosphoserine phosphatase22.88
(sera)
M368 HS H-P0653 nonheme iron-containing18.48
ferritin
(Pfi')
M379 G2 H-P0654-2 conserved hypothetical39.71
protein
M379 H2 H-P0655-2 protective surface 100.87
antigen D15
M368 A6 H-P0656 conserved hypothetical42.24
protein
M368 B6 H-P0657 processing protease 47.63
(ymxG)
M368 C6 H-P0658 PET112-like protein 52.36
M368 D6 H-P0659 hypothetical protein 45.65
M368 E6 H-P0660 hypothetical protein 37.29
M368 F6 H-P0661 ribonuclease H (rnhA)15.84
M368 G6 H-P0662 ribonuclease III (rnc)26.51
M368 H6 H-P0663 chorismate synthase 40.26
(aroC)
M368 A7 H-P0665 oxygen-independent 50.38
coproporphyrinogen
III oxidase
(hemN)
M368 B7 H-P0667 hypothetical protein 9.46
M368 C7 H-P0668 hypothetical protein 66.88
M368 D7 H-P0671 outer membrane protein29.81
(ompl4)
M368 E7 H-P0672 solute-binding signature43.01
and
mitochondrial signature
protein
(~pB)
M379 B3 H-P0673-2 hypothetical protein 46.97
M381 H3 H-P0674 hypothetical protein 25.19
M368 F? H-P0676 methylated-DNA--protein-18.59
cysteine methyltransferase
(datl)
M368 G7 H-P0677 conserved hypothetical28.16
integral
membrane protein
M368 H7 H-P0679 lipopolysaccharide 31.9
biosynthesis
protein (wbpB)
M369 A1 H-P0681 hypothetical protein 18.59
M369 BI H-P0682 hypothetical protein 13.97
M369 Cl H-P0683 UDP-N-acetylglucosamine47.74
pyrophosphorylase
(ghnU)
M369 D1 H-P0685 flagellar biosynthetic19.03
protein
(flip)
M369 E1 H-P0687 iron(II) transport 70.73
protein (feoB)
M369 F1 H-P0688 hypothetical protein 18.37
M369 G H-P0690 acetyl coenzyme A 43.12
1 acetyltransferase
(thiolase) (fadA)
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M381 A1 H-P0691 3-oxoadipate coA-transferase25.63
_subunit A (yxjD)
M381 B1 H-P0692 3-oxoadipate coA-transferase22.88
subunit B (yxjE)
M369 Hl H-P0694 hypothetical protein28.38
M369 A2 H-P0695 hydantoin utilization78.54
protein A
(hyuA)
M369 B2 H-P0697 hypothetical protein18.59
M369 C2 H-P0699 hypothetical protein37.73
M369 D2 H-P0700 diacylglycerol kinase14.19
(dgkA)
M369 E2 H-P0701 DNA gyrase, sub A 91.08
(gyrA)
M369 F2 H-P0703 response regulator 42.02
M369 G2 H-P0707 conserved hypothetical33.99
protein
M369 H2 H-P0711 hypothetical protein44.77
M369 A3 H-P0715 ABC transporter, 26.51
ATP-binding
protein
M369 B3 H-P0716 conserved hypothetical14.74
protein
M369 C3 H-P0718 conserved hypothetical23.21
integral
membrane protein
M369 D3 H-P0719 hypothetical protein12.1
M369 E3 H-P0723 L-asparaginase II 36.41
(ansB)
M369 F3 H-P0724 anaerobic C4-dicarboxylate48.84
transport protein
(dcuA)
M369 G3 H-P0727 transcriptional regulator,36.19
putative
M369 H3 H-P0728 conserved hypothetical37.07
protein
M369 A4 H-P0730 hypothetical protein11.22
M369 B4 H-P0732 hypothetical protein13.09
M369 C4 H-P0734 conserved hypothetical48.4
protein
M369 D4 H-P0735 xanthine guanine 16.94
phosphoribosyl
transferase (gpt)
M369 E4 H-P0737 conserved hypothetical17.49
integral
membrane protein
M381 H2 H-P0738 D-alanine:D-alanine 38.28
ligase A
(ddlA)
M369 F4 H-P0739 2-hydroxy-6-oxohepta-2,4-26.62
dienoate hydrolase
M369 G4 H-P0741 conserved hypothetical17.82
protein
M369 H4 H-P0745 conserved hypothetical36.08
protein
M369 AS H-P0747 conserved hypothetical43.34
protein
M369 BS H-P0748 cell division protein24.64
(ftsE)
M369 CS H-P0749 cell division membrane29.59
protein
(ftsX)
M369 DS H-P0750 hypothetical protein44.11
M369 ES H-P0752 flagellar hook-associated74.25
protein
2 (flip)
M381 F3 H-P0755 molybdopterin biosynthesis23.21
protein (moeB)
M379 C3 H-P0757-2 beta-alanine synthetase32.23
homolog
M369 FS H-P0758 conserved hypothetical48.18
integral
membrane protein
M369 GS H-P0759 conserved hypothetical45.98
integral
membrane protein
M369 HS H-P0761 hypothetical protein22.11
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M369 A6 H-P0762 hypothetical protein20.46
M369 H6 H-P0767 hypothetical protein2.75
M369 C6 H-P0768 molybdenum cofactor 35.42
biosynthesis protein
A (moaA)
M369 D6 H-P0769 molybdopterin-guanine22.22
dinucleotide biosynthesis
protein
A (mobA)
M369 E6 H-P0771 hypothetical protein27.06
M369 F6 H-P0772 N-acetylmuramoyl-L-alanine48.51
amidase (amiA)
M369 G6 H-P0773 hypothetical protein40.04
M369 H6 H-P0777 uridine 5'-monophosphaie26.51
(UMP)
kinase (pyres)
M370 A1 H-P0782 hypothetical protein50.16
M370 B1 H-P0783 hypothetical protein18.26
M370 C1 H-P0792 sigma-54 interacting55.77
protein
M370 D1 H-P0793 polypeptide deformylase19.25
(def)
M370 E1 H-P0794 ATP-dependent clp 21.67
protease
proteolytic component
(clpP)
M370 F1 H-P0796 outer membrane protein30.69
(ompl8)
M379 G3 H-P0797-2 flagellar sheath 28.71
adhesin hpaA
M379 H3 H-P0798-2 molybdenum cofactor 17.49
biosynthesis protein
C (moaC)
M370 G H-P0799 molybdopterin biosynthesis19.47
1 protein (mog)
M370 H1 H-P0800 molybdopterin converting16.06
factor,
subunit 2 (moaE)
M379 A4 H-P0801-2 molybdopterin converting8.25
factor,
subunit 1 (moaD)
M379 B4 H-P0802-2 GTP cyclohydrolase 21.23
II (ribA)
M379 D3 H-P0803-2 hypothetical protein30.8
M379 E3 H-P0804-2 GTP cyclohydrolase 37.95
II/3,4-
dihydroxy-2-butanone
4-
phosphate synthase
(ribA, ribB)
M379 F3 H-P0805-2 lipooligosaccharide 31.35
SG8 epitope
biosynthesis-associated
protein
(lex2B)
M370 A2 H-P0806 hypothetical protein22.77
M379 C4 H-P0807-2 iron(III) dicitrate 86.68
transport protein
(fecA)
M370 B2 H-P0808 bolo-acp synthase 13.2
(acpS)
M370 C2 H-P0809 hypothetical protein20.24
M370 D2 H-PO810 conserved hypothetical22.11
protein
M370 E2 H-P0811 hypothetical protein11.99
M370 F2 H-P0812 hypothetical protein37.07
M370 G2 H-P0813 conserved hypothetical22.66
protein
M370 H2 H-P0814 thiamin biosynthesis28.16
protein
(thiF)
M370 A3 H-P0815 flagellar motor rotation28.38
protein
(motA)
M370 B3 H-P0831 conserved hypothetical21.67
ATP
binding protein
M379 D4 H-P0832-2 spermidine synthase 28.93
(speE)
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M379 E4 H-P0833-2 hypothetical protein 32.23
M37U C3 H-P0834 GTP-binding protein 50.49
homologue
(yphC)
M370 D3 H-P0835 histone-like DNA-binding10.45
protein
~ ~uP)
M370 E3 H-P0836 hypothetical protein 13.2
M370 F3 H-P0837 hypothetical protein 11.33
M370 G3 H-P0838 hypothetical protein 22.66
M370 H3 H-P0839 outer membrane protein64.68
Pl
(ompPl)
M370 A4 H-P0840 flaAl protein 36.74
M370 B4 H-P0841 pantothenate metabolism46.86
flavoprotein (dfp)
M370 C4 H-P0843 thiamin phosphate 24.2
pyrophosphorylase/hyroxyethylthi
azole kinase (thiB)
M370 D4 H-P0845 thiamin phosphate 30.14
pyrophosphorylase/hyroxyethylthi
azole kinase (thiM)
M370 E4 H-P0850 type I restriction 58.08
enzyme M
protein (hsdM)
M370 F4 H-P0851 conserved hypothetical25.08
integral
membrane protein
M370 G4 H-P0854 GMP reductase (guaC) 36.08
M370 H4 H-P0858 ADP-heptose synthase 50.82
(rfaE)
M370 AS H-P0859 ADP-L-glycero-D-mannoheptose-36.41
6-epimerase (rfaD)
M370 BS H-P0861 hypothetical protein 27.17
M370 CS H-P0862 hypothetical protein 24.64
M379 F4 H-P0863-2 hypothetical protein 59.73
M370 DS H-P0865 deoxyuridine 5'-triphosphate16.06
nucleotidohydrolase
(dut)
M370 ES H-P0866 transcription elongation18.15
factor
GreA (greA)
M379 G4 H-P0867-2 lipid A disaccharide 39.71
synthetase
(1P~)
M379 H4 H-P0870-2 flagellar hook (flgE)79.09
M370 FS H-P0871 CDP-diglyceride hydrolase26.95
(cdh)
M370 GS H-P0872 alkylphosphonate uptake12.I
protein
(P~)
M370 HS H-P0873 hypothetical protein 7.92
M371 A1 H-P0879 hypothetical protein 22.33
M371 B1 H-P0883 Holliday junction 20.24
DNA helicase
(ruvA)
M371 Cl H-P0885 virulence factor mviN50.82
protein
(mviN)
M371 D H-P0886 cysteinyl-tItNA synthetase5126
1 (cysS)
M371 E1 H-P0889 iron(III) dicitrate 35.97
ABC
transporter, permease
protein
(fecD)
M371 F1 H-P0890 conserved hypothetical28.27
protein
M371 G H-P0891 conserved hypothetical19.25
I protein
M371 H1 H-P0892 conserved hypothetical10.01
protein
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M371 A2 H-P0894 conserved hypothetical9.79
protein
M371 B2 H-P0895 hypothetical protein13.86
M371 C2 H-P0896 outer membrane protein77.99
(omp 19)
M371 D2 H-P0897 hypothetical protein22.99
M371 E2 H-P0898 hydrogenase expression/formation40.81
protein (hypD)
M371 F2 H-P0899 hydrogenase expression/formation8.58
protein (hypC)
M371 G2 H-P0900 hydrogenase expression/fonnation26.73
protein (hypB)
M371 H2 H-P0905 phosphotransacetylase24.64
(pta)
M371 A3 H-P0906 hypothetical protein58.08
M371 B3 H-P0907 _ 33.22
hook assembly protein,
flagella
(B!~)
M371 C3 H-P0909 hypothetical protein22.22
M371 D3 H-P0912 outer membrane protein56.76
(omp20)
M371 E3 H-P0913 outer membrane protein58.3
(omp21)
M371 F3 H-P0914 hypothetical protein56.65
M371 G3 H-P0915 iron-regulated outer61.93
membrane
protein (frpB)
M371 H3 H-P0916 iron-regulated outer27.5
membrane
protein (frpB)
M380 A1 H-P0917-2 hypothetical protein2.64
M371 A4 H-P0918 hypotheticai protein15.84
M371 B4 H-P0920 conserved hypothetical25.41
integral
membrane protein
M371 C4 H-P0921 glyceraldehyde-3=phosphate36.63
dehydrogenase(gap)
M371 D4 H-P0923 outer membrane protein40.7
(omp22)
M371 E4 H-P0925 recombinational DNA 21.34
repair
protein (recR)
M371 F4 H-P0927 heat shock protein 35.97
(htpX)
M371 G4 H-P0928 GTP cyclohydrolase 19.91
I (folE)
M371 H4 H-P0929 geranyltranstransferase33.44
(ispA)
M371 A5 H-P0930 stationary-phase 29.48
survival protein
(surf)
M371 BS H-P0931 hypothetical protein16.17
M371 CS H-P0932 hypothetical protein11.1
l
M371 DS H-P0933 hypothetical protein22.11
M371 ES H-P0934 conserved hypothetical27.72
protein
M371 F5 H-P0935 hypothetical protein17.82
M371 GS H-P0936 proline/betaine transporter42.9
(prop)
M371 HS H-P0938 hypothetical protein12.76
M371 A6 H-P0939 amino acid ABC transporter,26.18
permease protein
(yckJ)
M371 B6 H-P0940 amino acid ABC transporter,28.27
periplasmic binding
protein
(Yc~)
M371 C6 H-P0941 alanine racemase, 41.58
biosynthetic
(alr)
M371 D6 H-P0942 D-alanine glycine 49.61
permease
{dagA)
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M371 E6 H-P0943 D-amino acid dehydrogenase45.21
{dadA)
M371 F6 H-P0944 translation initiation13.86
inhibitor,
putative
M371 G6 H-P0946 conserved hypothetical54.67
integral
membrane protein
M371 H6 H-P0947 hypothetical protein13.31
M371 A7 H-P0949 conserved hypothetical16.61
secreted
protein
M371 B7 H-P0950 acetyl-CoA carboxylase31.9
beta
subunit (accD)
M371 C7 H-P0951 hypothetical protein22.66
M371 D7 H-P0952 conserved hypothetical24.09
integral
membrane protein
M371 E7 H-P0953 hypothetical protein20.79
M371 F7 H-P0955 prolipoprotein diacylglyceryl31.35
transferase (lgt)
M371 G7 H-P0956 conserved hypothetical26.73
protein
M371 H7 H-P0957 3-deoxy-d-manno-octulosonic-43.34
acid transferase
(kdtA)
M371 A8 H-P0958 hypothetical protein28.05
M371 B8 H-P0960 glycyl-tIZIVA synthetase,33.44
alpha
subunit (glyQ)
M371 C8 H-P0961 glycerol-3-phosphate34.43
dehydrogenase (NAD(P)+)
M380 B H-P0965-2 hypothetical protein48.84
1
M371 D8 H-P0966 conserved hypothetical60.5
protein
M380 F1 H-P0968-2 hypothetical protein2.42
M371 E8 H-P0969 cation efflux system112.31
protein
(czcA)
M371 F8 H-P0970 nickel-cobalt-cadmium39.6
resistance
protein {nccB)
M371 G8 H-P0971 hypothetical protein45.54
M371 H8 H-P0972 glycyl-tlZlVA synthetase,77.22
beta
subunit (glyS)
M371 A9 H-P0973 hypothetical protein38.94
M380 C1 H-P0974-2 phosphoglycerate 54.12
mutase (pgm)
M380 D1 H-P0975-2 conserved hypothetical10.34
protein
M380 EI H-P0976-2 adenosyhnethionine-8-amino-7-48.07
oxononanoate aminotransferase
(bioA)
M380 H1 H-P0994-2 hypothetical protein29.48
M380 G1 H-P1000-2 PARA protein 24.09
M380 A2 H-P1001-2 hypothetical protein10.45
M380 B2 H-P1002-2 hypothetical protein43.45
M380 C2 H-P1003-2 hypothetical protein40.81
M380 D2 H-P1004-2 hypothetical protein30.14
M380 E2 H-P1005-2 hypothetical protein11.55
M380 F2 H-P1006-2 conjugal transfer 19.58
protein (traG)
M380 G2 H-P1017-2 amino acid permease 57.2
(rocE)
M380 H2 H-P1042-2 hypothetical protein38.39
M380 A3 H-P1056-2 hypothetical protein31.35
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M380 B3 H-P1075-2 conserved hypothetical48.29
secreted
protein
M373 A H-P 1076 hypothetical protein18.92
1
M373 B1 H-P1077 nickel transport 36.52
protein (nixA)
M373 C H-P 1080 conserved hypothetical20.9
1 integral
membrane protein
M373 D1 H-P1081 hypothetical protein22.88
M373 E1 H-P1082 multidrug resistance60.72
protein
(msbA)
M373 F1 H-P1083 hypothetical protein52.8
M373 GI H-P1084 aspartate transcarbamoylase33.88
(P3'rB)
M373 H1 H-P1085 hypothetical protein18.92
M373 A2 H-P1086 hemolysin (tly) 25.96
M373 B2 H-P1087 riboflavin biosynthesis30.91
regulatory
protein (ribC)
M373 C2 H-P1088 transketolase A (tktA)70.62
M373 D2 H-P1091 alpha-ketoglutarate 46.97
permease
(kgtP)
M373 E2 H-P1092 flagellar basal-body29.7
rod protein
{flgG)
M373 F2 H-P1096 IS605 transposase 15.73
(tnpA)
M373 G2 H-P1098 conserved hypothetical32.01
secreted
protein
M373 H2 H-P1101 glucose-6-phosphate 46.86
dehydrogenase(g6pD)
M373 A3 H-P1102 glucose-6-phosphate 25.08
1-
dehydrogenase{devB)
M373 B3 H-P1103 glucokinase (glk) 37.07
M373 C3 H-P1108 pyruvate ferredoxin 20.57
oxidoreductase, gamma
subunit
M373 D3 H-P1109 pyruvate ferredoxin 14.41
oxidoreductase, delta
subunit
M373 E3 H-P1110 pyruvate ferredoxin 44,88
oxidoreductase, alpha
subunit
M373 F3 H-P1111 pyruvate ferredoxin 34.65
oxidoreductase, beta
subunit
M373 G3 H-P1112 adenylosuccinate 48.51
lyase (purB)
M380 C3 H-P1113-2 outer membrane protein30.58
(omp24)
M373 H3 H-P 1117 conserved hypothetical28.27
secreted
protein
M373 A4 H-P1120 hypothetical protein15.95
M373 B4 H-P1121 cytosine specific 34.43
DNA
methyltransferase
(BSP6IM)
M380 D3 H-P1122-2 hypothetical protein8,47
M373 C4 H-P1123 peptidyl-prolyl cis-traps20.46
isomerase, FKBP-type
rotamase
(slyD)
M373 D4 H-P1124 hypothetical protein36.52
M373 E4 H-P1125 peptidoglycan associated19.8
lipoprotein precursor
(ompl8)
M373 F4 H-P1126 colicin tolerance-like45.98
protein
(tolB)
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M373 G4 H-P1128 hypothetical protein 9.35
M373 H4 H-P1129 biopolymer transport 14.74
protein
(exbD)
M373 AS H-P1131 ATP synthase F1, subunit13.?5
epsilon
(atpC)
M373 BS H-P1134 ATP synthase F1, subunit55.44
alpha
(atpA)
M373 CS H-P1135 ATP synthase F1, subunit19.91
delta
(atpH)
M373 DS H-P1137 ATP synthase F0, subunit15.95
b'
(atpF')
M373 ES H-P1138 plasmid replication-partition32.01
related protein
M373 FS H-P1139 SpoOJ regulator (soj)29.15
M373 GS H-P1140 biotin operon repressor/biotin23.43
acetyl coenzyme A
carboxylase
synthetase (birA)
M373 HS H-P1141 methionyl-tltNA 33.44
fonmyltransferase
(fret)
M373 A6 H-P1144 hypothetical protein 9.46
M373 B6 H-Pl i45 hypothetical protein 11.44
M373 C6 H-P1147 ribosomal protein 13.09
L19 (rp119)
M373 D6 H-P1148 tRNA (guanine-Nlr 25.3
methyltransferase
(trmD)
M373 E6 H-P1149 conserved hypothetical20.35
protein
M380 F3 H-P1150-2 hypothetical protein 12.76
M373 F6 H-PI 152 signal recognition 49.39
particle protein
M380 G3 H-P1153-2 valyl-tItNA synthetase96.25
(valS)
M380 E3 H-P115?-2 outer membrane protein135.41
(omp26)
M373 G6 H-P1158 pyrroline-5-carboxylate28.38
reductase
(Pry)
M373 H6 H-P1159 cell filamentation 19.58
protein (fic)
M373 A7 H-P1160 conserved hypothetical15.51
protein
M380 A4 H-P1163-2 hypothetical protein 7.04
M373 B7 H-P1165 tetracycline resistance42.57
protein
tetA(P), putative
M373 C7 H-P1168 carbon starvation 75.68
protein (cstA)
M373 D7 H-P1169 glutamine ABC transporter,23.98
permease protein (glnP)
M380 H3 H-P1169-2 glutamine ABC transporter,23.98
permease protein (glnP)
M374 A1 H-P1170 glutamine ABC transporter,24.64
permease protein (ghiP)
M374 B1 H-P1171 glutamine ABC transporter,27.39
ATP-
binding protein (glnQ)
M374 CI H-P1 I72 glutamine ABC transporter,30.58
periplasmic glutamine-binding
protein (glnH)
M374 D1 H-P1173 hypothetical protein 20.24
M374 E1 H-P1174 glucose%galactose 44.88
transporter
(glue)
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M374 F1 H-P1175 conserved hypothetical47.96
integral
membrane protein
M374 G1 H-PI 177 outer membrane protein70.62
(omp27)
M374 H1 H-P1178 purine-nucleoside 25.74
phosphorylase
(deoD)
M374 A2 H-P1179 phosphopentomutase 45.54
(deoB)
M374 B2 H-Pl 180 pyrimidine nucleoside46.09
transport
protein (nupC)
M374 C2 H-P1183 NA+/H+ antiporter 42.24
(napA)
M374 D2 H-P1184 conserved hypothetical50.6
integral
membrane protein
M374 E2 H-P1185 conserved hypothetical43.12
integral
membrane protein
M374 F2 H-P1186 carbonic anhydrase 22.33
M374 G2 H-P1187 hypothetical protein 42.46
M374 H2 H-P1188 hypothetical protein 29.7
M374 A3 H-P1189 aspartate-semialdehyde38.17
dehydrogenase (asd)
M374 B3 H-PI 191 ADP-heptose-lps 38.5
heptosyltransferase
II (rfaF)
M374 C3 H-P1196 ribosomal protein 17.16
S7 (rps7)
M374 D3 H-P1200 ribosomal protein 18.15
L10 (rp110)
M374 E3 H-P 1201 ribosomal protein 25.85
L 1 (rpl l )
M374 F3 H-P1202 ribosomal protein 15.62
LI1 (rpll I)
M374 G3 H-P1203 transcription termination19.47
factor
NusG (nusG)
M380 B4 H-P1205-2 translation elongation44
factor EF-
Tu (tufB)
M374 H3 H-P1206 multidrug resistance 63.69
protein
(hetA)
M374 A4 H-PI207 hypothetical protein 24.53
M374 B4 H-P1210 serine acetyltransferase18.92
(cysE)
M380 F4 H-P1213-2 polynucieotide phosphorylase75.79
- (pnP)
M380 G4 H-P1214-2 conserved hypothetical26.51
protein
M380 C4 H-P1215-2 hypothetical protein 8.91
M380 D4 H-P1216-2 conserved hypothetical72.71
secreted
protein
M380 E4 H-P1217-2 hypothetical protein 17.6
M374 C4 H-P1220 ABC transporter, ATP-binding25.19
protein (yhcG)
M374 D4 H-P1221 conserved hypothetical25.85
protein
M374 E4 H-P1222 D-lactate dehydrogenase104.39
(dld)
M374 F4 H-P1224 uroporphyrinogen III 24.97
cosynthase
(hemp)
M374 G4 H-P1225 conserved hypothetical14.41
integral
membrane protein
M374 H4 H-P1226 oxygen-independent 38.83
coproporphyrinogen
III oxidase
(hemN)
M380 H4 H-P1227-2 cytochrome c553 10.67
M380 AS H-P1228-2 invasion protein (invA)17.16
M380 BS H-P1229-2 aspartokinase (lysC) 44.66
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M374 AS H-P1230 hypo_thet_ical protein19.91
M374 BS H-P1231 DNA polymerise III 24.09
delta prime
subunit (holB)
M374 CS H-P1232 dihydropteroate synthase41.91
(folP)
M380 DS H-P1233-2 hypothetical protein 16.94
M374 DS H-P1234 conserved hypothetical32.89
integral
membrane protein
M374 E5 H-P1235 conserved hypothetical45.76
integral
membrane protein
M374 FS H-P1236 hypothetical protein 20.24
M3?4 GS H-P1237 carbamoyl-phosphate 41.36
synthetase
(pyrAa)
M374 HS H-P1240 conserved hypothetical21.01
protein
M380 CS H-P1241-2 alanyl-tltNA synthetase93.28
(alas)
M374 A6 H-P1242 conserved hypothetical8.47
protein
M380 HS H-P1243-2 outer membrane protein80.74
(omp28)
M374 B6 H-P1244 ribosomal protein 9.46
S18 (rpsl8)
M374 C6 H-P1245 single-strand DNA-binding19.8
protein (ssb)
M374 D6 H-P1246 ribosomal protein 15.73
S6 (rps6)
M380 A6 H-PI247-2 hypothetical protein 37.51
M374 E6 H-P1248 virulence associated 70.95
protein
homolog (vacB)
M380 B6 H-P1249-2 shikimate 5-dehydrogenase29.04
(aroE)
M380 ES H-P1251-2 oligopeptide ABC transporter,38.39
permease protein (oppB)
M380 FS H-P1252-2 oligopeptide ABC transporter,65.45
periplasmic oligopeptide-binding
protein (oppA)
M380 GS H-P1253-2 tryptophanyl-tltNA 37.4
synthetase
(trpS)
M374 F6 H-P1254 biotin synthesis protein26.51
(bioC)
M374 G6 H-P1255 protein translocation2222
protein, low
temperature (sect)
M374 H6 H-P1256 ribosome releasing 20.46
factor (frr)
M374 A7 H-P1257 orotate phosphoribosyltransferase22.22
(pyrE)
M374 B7 H-P1258 conserved hypothetical17.05
mitochondria) protein
4
M374 C7 H-P1260 NADH-ubiquinone 14.74
oxidoreductase, NQ07
subunit
(NQO7)
M374 D7 H-P1262 NADH-ubiquinone 29.37
oxidoreductase, NQOS
subunit
(NQOS)
M374 E7 H-P1263 NADH-ubiquinone 45.1
oxidoreductase, NQ04
subunit
(NQ04)
M380 C6 H-P1264-2 hypothetical protein 8.47
M374 F7 H-P1265 hypothetical protein 36.19
M375 A1 H-P1268 NADH-ubiquinone 24.31
oxidoreductase, NQ09
subunit
(NQ09)
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M375 B1 H-P1275 phosphomannomutase 50.6
(algC)
M375 C1 H-P1277 tryptophan synthase,28.93
alpha
subunit (trpA)
M375 D1 H-P1278 tryptophan synthase,43.34
beta subunit
(trpB)
M375 E1 H-P1279 anthranilate isomerase49.83
(trpC)
M375 F1 H-P1282 anthranilate synthase55.11
component I
(trpE)
M375 G1 H-P1285 conserved hypothetical25.41
secreted
protein
M375 H1 H-P1286 conserved hypothetical20.13
secreted
protein
M375 A2 H-P1287 transcriptional regulator23.98
(tenA)
M375 B2 H-P1288 hypothetical protein14.63
M375 C2 H-P1289 hypothetical protein17.82
M375 D2 H-P1290 nicotinamide mononucleotide24.31
transporter {pnuC)
M375 E2 H-P1291 conserved hypothetical22.55
protein
M375 F2 H-P1292 ribosomal protein 12.87
L17 (rp117)
M375 G2 H-P1293 DNA-directed RNA 37.95
polymerise,
alpha subunit (rpoA)
M375 H2 H-P1294 ribosomal protein 22.99
S4 (rps4)
M375 A3 H-P1295 ribosomal protein 14.52
S11 (rpsl l)
M375 B3 H-P1296 ribosomal protein 13.31
S13 (rpsl3)
M380 D6 H-P1298-2 translation initiation8.03
factor EF-1
(infA)
M375 C3 H-P1299 methionine amino 27.94
peptidase
(map)
M375 D3 H-P1302 ribosomal protein 16.94
SS {rps5)
M375 E3 H-P1303 ribosomal protein 13.2
L18 (rp118)
M375 F3 H-P1305 ribosomal protein 14.52
S8 (rps8)
M375 G3 H-P1307 ribosomal protein 20.02
LS (rpl5)
M375 H3 H-P1308 ribosomal protein 8.14
L24 (rp124)
M375 A4 H-P1309 ribosomal protein 13.53
L14 (rp114)
M375 B4 H-P1310 ribosomal protein 9.57
S17 (rpsl7)
M375 C4 H-P1312 ribosomal protein 15.62
L16 (rp116)
M375 D4 H-P1314 ribosomal protein 13.53
L22 (rp122)
M375 E4 H-P1315 ribosomal protein 10.34
S19 (rpsl9)
M375 F4 H-P1318 ribosomal protein 23.76
L4 (rpl4)
M375 G4 H-P1319 ribosomal protein 21.12
L3 (rpl3)
M375 H4 H-P1320 ribosomal protein 11.55
S10 (rpsl0)
M375 AS H-P1321 conserved hypothetical41.58
ATP-
binding protein
M375 BS H-P1322 hypothetical protein22.22
M375 CS H-P1323 ribonuclease HII 23.1
(rnhB)
M375 DS H-P1324 hypothetical protein9.24
M375 ES H-P1326 hypothetical protein13.86
M375 FS H-P1327 hypothetical protein45.43
M375 GS H-P1328 cation efflux system37.29
protein
(czcA)
M375 HS H-P1330 ~ conserved hypothetical12.76
~ integral
membrane protein
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M375 A6 H-P1331 conserved hypothetical25.19
integral
membrane protein
M375 B6 H-P1332 co-chaperone and heat40.7
shock
protein (dnal7
M375 C6 H-P1333 hypothetical protein 42.13
M375 D6 H-PI335 conserved hypothetical39.71
protein
M375 E6 H-P1336 hypothetical protein 27.94
M375 F6 H-P1337 conserved hypothetical19.25
protein
M375 G6 H-P1338 conserved hypothetical16.39
protein
M375 H6 H-P1340 biopolymer transport 14.3
protein
(exbD)
M375 A7 H-P1341 siderophore-mediated 31.46
iron
transport protein
(tong)
M375 B7 H-P1342 outer membrane protein76.12
(omp29)
M375 C7 H-P1343 conserved hypothetical26.73
integral
membrane protein
M375 D7 H-P1344 magnesium and cobalt 35.09
transport
protein (corA)
M375 E7 H-P1345 phosphoglycerate kinase44.33
M375 F7 H-P1346 glyceraldehyde-3-phosphate36.41
dehydrogenase(gap)
M375 G7 H-P1347 uracil-DNA glycosylase25.74
(ung)
M375 H7 H-P1349 hypothetical protein 42.68
M375 A8 H-P1350 protease 50.6
M375 B8 H-P1355 nicotinate-nucleotide30.14
pyrophosphorylase
(nadC)
M375 C8 H-P1356 quinolinate synthetase37.07
A (nadA)
M375 D8 H-P1357 phosphatidylserine 29.48
decarboxylase
proenzyme (psd)
M375 E8 H-P1358 hypothetical protein 18.59
M375 F8 H-P1360 4-hydroxybenzoate 32.45
octaprenyltransferase
(ubiA)
M375 G8 H-P1361 competence locus E 45.98
(comE3)
M375 H8 H-P1362 replicative DNA helicase53.79
(dnaB)
M375 A9 H-P1363 conserved hypothetical51.37
integral
membrane protein
M376 A1 H-P1364 signal-transducing 43.78
protein,
histidine kinase
M376 B1 H-P1365 response regulator 23.54
M376 C1 H-P1371 type III restriction 106.59
enzyme R
protein
M376 D1 H-P1372 rod shape-determining27.39
protein
(mreC)
M376 E1 H-P1373 rod shape-determining38.28
protein
(mreB)
M376 Fl H-P1374 ATP-dependent protease49.17
ATPase
subunit (clpX)
M376 G1 H-PI375 UDP-N-acetylglucosamine29.81
acyltransferase (IpxA)
M376 H1 H-P1376 (3R)-hydroxymyristoyl-(acyl17.6
carrier protein) dehydratase
(fabZ)
~ M376 A2 H-P1377 ~ hypothetical protein 16.17
~
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M376 B2 H-P1378 competence lipoprotein24.31
(comL)
M376 C2 H-P1379 ATP-dependent protease91.96
(lon)
M376 D2 H-PI380 prephenate dehydrogenase29.26
(tyrA)
M381 C1 H-P1381 hypothetical protein g.5g
M376 E2 H-P1382 hypothetical protein 14.41
M376 F2 H-P1383 restriction modification17.71
system S
subunit
M376 G2 H-P1384 hypothetical protein 7.59
M376 H2 H-P1385 fructose-1,6-bisphosphatase32.01
M376 A3 H-P1386 D-ribulose-5-phosphate23.98
3
epimerase (rpe)
M376 B3 H-P1388 hypothetical protein 16.5
M376 C3 H-P1389 hypothetical protein 6,71
M376 D3 H-P1390 hypothetical protein 18.37
M376 E3 H-P1391 hypothetical protein 10.89
M376 F3 H-PI392 fbronectin/fibrinogen-binding47.96
protein
M376 G3 H-P1393 DNA repair protein 57.75
(recN)
M376 H3 H-P1394 conserved hypothetical31.35
protein
M376 A4 H-P1395 outer membrane protein26.73
(omp30)
M376 B4 H-P1396 hypothetical protein 31.79
M376 C4 H-P1398 alanine dehydrogenase41.91
(ald)
M376 D4 H-P1399 arginase (rocF) 35.53
M376 E4 H-P1400 iron(III) dicitrate 92.73
transport protein
(fecA)
M376 F4 H-P1401 conserved hypothetical25.96
protein
M381 A2 H-P1402 type I restriction 109.34
enzyme R
protein (hsdR)
M381 B2 H-P1403 type I restriction 89.98
enzyme M
protein (hsdM)
M376 G4 H-P1405 hypothetical protein 3.85
M376 H4 H-P1406 biotin synthetase 31.13
(bioB)
M376 AS H-P1407 conserved hypothetical32.23
integral
membrane protein
M381 C2 H-P1408 hypothetical protein 12.32
M381 D2 H-P1409 hypothetical protein 63.69
M376 BS H-P1410 hypothetical protein 43.45
M376 CS H-P1411 hypothetical protein 68.2
M376 DS H-P1412 hypothetical protein 33.99
M376 ES H-P1413 conserved hypothetical16.39
protein
M376 FS H-P1414 conserved hypothetical12.54
protein
M376 GS H-P1415 tRNA delta(2)- 29.37
isopentenylpyrophosphate
transferase (miaA)
M376 HS H-P1418 UDP-N- 28.6
acetylenolpyruvoylglucosamine
reductase (murB)
M376 A6 H-P1419 flagellar biosynthetic9.79
protein
(fliQ)
M376 86 H-P1420 flagellar export protein47.85
ATP
synthase (fliI)
M376 C6 H-P1421 conjugative transfer 33.55
regulon
protein (trbB)
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M376 D6 H-P1423 conserved hypothetical9.35
protein
M376 E6 H-P1424 hypothetical protein 22.77
~
M376 F6 H-P1425 hypothetical protein 8.36
M376 G6 H-P1427 histidine-rich, metal6.71
binding
polypeptide (hpn)
M376 H6 H-P1428 conserved hypothetical39.38
protein
M376 A7 H-P1429 polysialic acid capsule36.3
expression
protein (lcpsF)
M376 B7 H-PI430 conserved hypothetical?5.9
ATP-
binding protein
M376 C7 H-P1431 16S rRNA (adenosine-N6,N6-)-29.92
dimethyltransferase
(ksgA)
M376 D7 H-P1432 histidine and glutamine-rich8.03
protein
M376 E7 H-P1433 hypothetical protein 94.27
M376 F7 H-P1434 formyltetrahydrofolate32.34
hydrolase
(purU)
M376 G7 H-P1435 protease IV (PspA) 32.23
M376 H7 H-P1436 hypothetical protein 9.13
M376 A8 H-P1438 conserved hypothetical37.29
lipoprotein
M376 B8 H-P1439 hypothetical protein 9.02
M376 C8 H-P1440 hypothetical protein 28.6
M376 D8 H-P1441 peptidyl-prolyl cis-trans18.04
isomerase B, cyclosporin-type
rotamase (ppi)
M376 E8 H-P1442 carbon storage regulator8.47
(csrA)
M376 F8 H-P1443 conserved hypothetical29.59
protein
M376 G8 H-P1444 small protein (smpB) 16.83
M376 H8 H-P1445 biopolymer transport 16.61
protein
(exbB)
M376 A9 H-P1446 biopolymer transport 14.74
protein
(exbD)
M376 B9 H-P1447 ribosomal protein 4.95
L34 (rp134)
M376 C9 H-P1448 ribonuclease P, protein17.82
component (mpA)
M376 D9 H-P1449 conserved hypothetical12.98
protein
M376 E9 H-P1450 60 kDa inner-membrane60.28
protein
M376 F9 H-P1451 hypothetical protein 29.15
M376 G9 H-P1452 thiophene and furan 50.82
oxidizer
(tdhF)
M376 H9 H-P1453 conserved hypothetical82.17
protein
M376 A10 H-P1454 hypothetical protein 33.44
M376 BIO H-P1455 hypothetical protein 14.41
M376 C10 H-P1456 membrane-associated 19.36
lipoprotein
(1pp20)
M376 D10 H-P1457 hypothetical protein 23.21
M376 E10 H-P1458 thioredoxin 11.55
M376 F10 H-P1461 cytochrome c551 peroxidase38.61
M377 AI H-P1462 secreted protein involved19.03
in
flagellar motility
M377 B1 H-P1463 hypothetical protein 24.86
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M377 CI H-P1464 conserved hypothetical29.92
secreted
protein
M377 D1 H-P1465 ABC transporter, ATP-binding28.82
protein (HI1087)
M377 E1 H-P1466 conserved hypothetical41:58
integral
membrane protein
M377 F1 H-P1467 hypothetical protein 25.52
M377 G1 H-P1468 branched-chain-amino-acid37.51
aminotransferase (ilvE)
M377 H1 H-P1469 outer membrane protein27.39
(omp31)
M377 A2 H-P1473 hypothetical protein 21.12
M377 B2 H-P1474 thymidylate kinase 21.12
(tmk)
M377 C2 H-P1475 lipopolysaccharide 17.38
core
biosynthesis protein
(kdtB)
M377 D2 H-P1476 phenylacrylic acid 20.68
decarboxylase
M377 E2 H-P1479 hypothetical protein 92.95
M377 F2 H-P1480 seryl-tRNA synthetase45.76
(serS)
M377 G2 H-P1481 hypothetical protein 29.26
M377 H2 H-P 1482 hypothetical protein 9.57
M377 A3 H-P1483 gerC2 protein (gerC2)27.17
M377 83 H-P1484 conserved hypothetical16.39
integral
membrane protein
M377 C3 H-P1485 proline dipeptidase 21.01
(pepQ)
M377 D3 H-P1486 conserved hypothetical41.47
integral
membrane protein
M377 E3 H-P1487 conserved hypothetical40.26
integral
membrane protein
M377 F3 H-P1488 conserved hypothetical36.3
secreted
protein
M377 G3 H-P1489 lipase-like protein 56.21
M381 G1 H-P1490 hemolysin 49.5
M377 H3 H-P1491 phosphate permease 58.74
M377 A4 H-P1492 conserved hypothetical9.9
nifU-like
protein
M377 B4 H-P1493 hypothetical protein 22.44
M377 C4 H-P1494 UDP-MurNac-tripeptide49.28
synthetase (murE)
M377 D4 H-P1495 transaldolase (tal) 34.87
M377 E4 H-P1496 general stress protein19.69
(ctc)
M377 F4 H-P1497 peptidyl-tRNA hydrolase20.57
(pth)
M377 G4 H-P1499 hypothetical protein 30.03
M377 H4 H=P1501 outer membrane protein42.79
(omp32)
M377 AS H-P1502 hypothetical protein 16.06
M377 BS H-P1503 cation-transporting 86.79
ATPase, P-
type (copA)
M377 CS H-P1504 conserved hypothetical26.29
protein
M377 DS H-P1505 riboflavin biosynthesis37.95
protein
(ribG)
M377 ES H-P1506 glutamate pennease 44.99
(gltS)
M377 FS H-P1507 conserved hypothetical42.46
ATP-
binding protein
M381 F2 H-P1508 ferrodoxin-like protein50.49
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M377 G5 H-P1509 conserved hypothetical28.93
integral
membrane protein
M377 H5 H-P1510 conserved hypothetical12.98
protein
M377 A6 H-P1511 hypothetical protein11.99
M377 B6 H-P1512 iron-regulated outer96.58
membrane
protein (fipB)
M377 C6 H-P1513 selenocystein synthase42.57
(selA)
M377 D6 H-P1514 transcription termination43.56
factor
NusA (nusA)
M377 E6 H-P1518 hypothetical protein10.56
M381 B3 H-P1521 type III restriction106.48
enzyme R
protein {res)
M381 C3 H-P1523 DNA recombinase (recta)68.64
M377 F6 H-P1524 hypothetical protein12.76
M377 G6 H-P1525 hypothetical protein23.32
M377 H6 H-P1526 exodeoxyribonuclease27.61
(IexA)
M377 A7 H-P1527 hypothetical protein52.8
M377 B7 H-P1530 purine nucleoside 19.91
phosphorylase
(Pub)
M377 C7 H-P1531 hypothetical proteing,g
M377 D7 H-P1532 glucosamine fructose-6-phosphate65.78
aminotransferase
(isomerizing)
(g~S)
M377 E7 H-PI533 conserved hypothetical25.52
protein
M377 F7 H-P1534 IS605 transposase 47.08
{tnpB)
M377 G7 H-P1535 IS605 transposase 15.73
(tnpA)
M377 H7 H-P1541 transcription-repair110
coupling
factor (trcF)
M377 A8 H-P1548 conserved hypothetical12.43
integral
membrane protein
M377 B8 H-P1551 conserved hypothetical14.08
secreted
protein
M377 C8 H-P 1552 Na+/H+ antiporter 48.29
(nhaA)
M381 B4 H-P1554 ribosomal protein 29.15
S2 (rps2)
M381 D4 H-P1555 translation elongation39.16
factor EF-
Ts (tsf)
M377 D8 H-P1556 cell division protein67.76
(ftsI)
M381 E4 H-P1557 flagellar basal-body12.1
protein (fliE)
M381 F4 H-P1558 flagellar basal-body17.82
rod protein
(flgC) (proximal
rod protein)
M381 G4 H-P1559 flagellar basal-body15.51
rod protein
(flgB) (proximal
rod protein)
M378 A1 H-P1560 cell division protein42.79
(fts~
M378 BI H-P1561 iron(III) ABC transporter,36.96
periplasmic iron-binding
protein
(ceuE)
M378 C1 H-P1562 iron(III) ABC transporter,36.74
periplasmic iron-binding
protein
(ceuE)
M378 D1 H-P1563 alkyl hydroperoxide 21.89
reductase
(tsaA)
~ M378 H-P1564 ~ outer membrane protein29.92
El ~
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M378 F1 H-P1565 penicillin-binding 64.79
protein 2
_" (pbp2)
M378 G 1 H-P 1566 hypothetical protein 16.28
M378 H1 _ conserved hypothetical22.99
H-P1567 ATP-
binding protein
M378 A2 H-P1568 hypothetical protein 20.24
M378 B2 H-P1569 hypothetical protein 21.78
M378 C2 H-P1570 conserved hypothetical18.15
protein
M378 D2 H-P1571 rare lipoprotein A 34.76
(rlpA)
M378 E2 H-P1572 regulatory protein 41.03
DniR
M378 F2 H-P1573 conserved hypothetical28.05
protein
M378 G2 H-P1576 ABC transporter, ATP-binding36.08
protein (abc)
M378 H2 H-P1577 ABC transporter, permease23.76
protein (yaeE)
M378 A3 H-P1580 hypothetical protein 24.31
M378 B3 H-P1581 methicillin resistance37.07
protein
(llm)
M378 C3 H-P1582 pyridoxal phosphate 28.93
biosynthetic
protein J (pdxJ)
M378 D3 H-P1583 pyridoxal phosphate 33.88
biosynthetic
protein A (pdxA)
M378 E3 H-P1584 sialoglycoprotease 37.51
(gcp)
M378 F3 H-P1585 flagellar basal-body 28.93
rod protein
(flgG)
M378 G3 H-P1587 conserved hypothetical17.16
protein
M378 H3 H-P1588 conserved hypothetical27.94
protein
M3 81 H H-P 1590 hypothetical protein 4.4
1
M318 G2 H-538729 sutoimmune antigen 67.1 67
Ku; p70
subunit
H 1 H-S39329 Kallikrein 1 24.64 30
(renal/pancreas/salivary)
{alternative products}
-
M270 G4 H-S43855 Recoverin, photoreceptor22.11 32.OkDa
protein
M300 C2 H-S56151 milk fat globule protein24.09 30
HMFG
M318 C1 H-S57153 retinoblastoma-binding101.31 101
protein 1,
isofonm I [RBBP1]
M271 B2 H-S57162 retinoblastoma-binding93.72 110
protein 1,
isoform III [RBBPI],
INTERACTS WITH THE
VIRAL
PROTEIN-BINDING DOMAIN
OF THE RETINOBLASTOMA
PROTEIN.
M3I7 H3 H-S62027 transducin, gamma 8.25 11
subunit
M270 G6 H-S66793 arrestin, X-arrestin=S-antigen42.79 SO.OkDa
homolog (human, retina,
mRNA,
1314 nt], MAY PLAY
A ROLE
IN AN AS YET UNDEFINED
RETINA-SPECIFIC SIGNAL
TRANSDUCTION.
M419 C2 H-567859 "transcription initiation48.360 64.OkDa
factor IIe,
alpha subunit"
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M302 D7 H-S69022 myosin, light polypeptide18.26 31
2,
ventricular
H5 H-569272 cytoplasmic antiproteinase=3841.47 50
kda intracellular
serine proteinase
inhibitor [human,
placenta,
mRNA, 1465 nt]
D 1 H-S72043 GIF=growth inhibitory7.59 19
factor
[human, brain, Genomic,
2015 nt]
M266 B3 H-S74221 cytokine IK factor 17.93 36.OkDa
D 1 H-574445 cellular retinoic 15.18 23
acid-binding
protein [human, skin,
mRNA, 735
nt]
E3 H-S74728 antiquitin=26g turgor56.32 53
protein
homolog [human, kidney,
mltNA,
1809 nt]
D4 H-S75174 E2F transcription 45.87 58
factor 4,
p I 07/p 130-binding
166-61 H-S76474 "trkB {alternately 55 52.54
spliced}
[human, brain, mRNA]"
169-40 H-S76617 "B lk=protein tyrosine60 55.62
kinase
[human, B lymphocytes,
mRNA,
2608 nt]"
M250 D3 H-S79522 ubiquitin carboxyl-terminal17.27 l7.OkDa
extension protein,
Ubiquitin A-52
residue ribosomal
protein fusion
product 1
M236 B4 H-S80562 calponin, acidic 36.3 49
G 1 H-S82470 BB 1=malignant cell 37.73 34
expression-
enhanced gene/tumor
progression-enhanced
gene
[human, UM-UC-9 bladder
carcinoma cell line,
mltNA, 1897
nt]
M313 E1 H-S85655 prohibitin [PHB] 30.03 40.OkDa
M465 A6 H-S87759 protein phosphatase 42.13 52.OkDa
2C alpha
[human, teratocarcinoma,
mltNA,
2346 nt]
M472 B1 H-000803 tyrosine-protein kinase55.620 64.OkDa
FItK
B2 H-002390 Human adenylyl cyclase-52.58 55
associated protein
homolog CAP2
(CAP2) mltNA, complete
cds
167-2 H-002680 human protein tyrosine36 38.57
kinase
mltNA
G2 H-U0305b Human tumor suppressor47.96 47
(LUCA-
I ) mIZIVA, complete
cds
M512 E3 H-003100 Human alpha2(E~catenin102.52 102.OkDa
mltNA,
complete cds
M306 G3 H-003187 72.93 95.OkDa
H3 H-003398 Human receptor 4-1BB 28.05 51
ligand
mRNA, complete cds
D3 H-003486 Human connexin40 gene,39.49 40
complete cds
M300 C3 H-003643 leukophysin 25.96 34
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FS H-003749 Human chromogranin 50.38 50
A (CHGA)
gene, promoter and
M314 C3 H-003886 GS2 (GB:U03886) 27.94 32.OkDa
M306 E3 H-004343 CD86 antigen (CD28 35.64 47
antigen
ligand 2, B7-2 antigen)
[CD86]
167-61 H-005012 TrkC 92 90.82
M302 GS H-005340 cell division cycle 55 55
protein p55
A4 H-005659 Hydroxysteroid (17-beta)34.21 36
dehydrogenase 3
F1 H-005861 Human hepatic dihydrodiol35.64 40
dehydrogenase gene
M302 B2 H-006452 antigen MART-I, melanoma13.09 20.OkDa
169-52 H-006454 human AMP-activated 70 60.79
protein
kinase (hAMPK) mRNA
M315 A3 H-006643 lectin, epidermal 15.07 18
H1 H-006715 Cytochrome B561 27.06 25
M476 ES H-007132 Human steroid hormone50.82 SS.OkDa
receptor
Ner-I mRNA, complete
cds
M236 D3 H-007151 guanine nucleotide-binding20.13 34
protein ADP-ribosylation
factor
like gene 3
M317 G3 H-007559 homeotic protein 38.17 38
Islet-1
M266 H1 H-007681 Human NAD(H)-specific40.37 40
isocitrate dehydrogenase
alpha
subunit precursor
mRNA,
complete cds
E3 H-007919 Aldehyde dehydrogenase56.43 53
6
M298 A3 H-008021 nicotinamide N-methyltransferase29.15 36.OkDa
M297 B1 H-008024 alcohol/hydroxysteroid31.46 SO.OkDa
sulfotransferase
A2 H-008336 Human basic helix-loop-helix21.89 42
transcription factor
mRNA,
complete cds
E2 H-009303 Human T cell leukemia38.17 40
LERK-2
(EPLG2) mRNA, complete
cds
M250 HS H-009559 RCHI, RAG (recombination58.3 58.OkDa
activating gene)
cohort 1
167-50 H-009564 human serine kinase 72 72.12
mRNA
166-74 H-009578 human MAPKAP kinase 50 42.09
(3pK)
mRNA
M302 C4 H-009813 ATP synthase, subunit15.73 30
9,
mitochondrial
A1 H-009850 Zinc forger protein 68.97 68
143 (clone
P~-I)
M423 EI H-009937 Human urokinase-type36.96 49.OkDa
plasminogen receptor
M450 H4 H-010117 Human endothelial-monocyte34.43 38.OkDa
activating polypeptide
II mRNA,
complete cds
M314 G H-U 10248 ribosomal protein 17.6 27
1 L29
M298 H H-U 10323 nuclear factor 45 44.77 45
I
E1 H-010492 Human Moxl protein 28.05 37
(MOX1)
mRNA, complete cds
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F3 H-010686 Human MAGE-11 antigen35.2 35
(MAGEl l) gene, complete
cds
167-38 H-011050 human NIMA-like protein55 49.02
kinase
1 (NLK1) mRNA
M266 B2 H-011292 Human Ki nuclear autoantigen29.48 32
mRNA, complete cds,
may play a
rol in cell adhesion
167-62 H-011791 human cyclin H m RNA 40 35.60
M423 DS H-U 12255 immunoglobulin gamma 40.26 48.OkDa
heavy
chain Fc receptor
RI, high affinity
M302 F7 H-U 12404 Csa-19 23.98 32
M236 A2 H-012465 ribosomal protein 13.64 24
L35
169-4 H-012535 human epidermal growth100 90.49
factor
receptor kinase substrate
(Eps8)
mRNA
F3 H-012597 Human tumor necrosis 55.22 64
factor type
2 receptor associated
protein
(TRAP3) mRNA, complete
cds
M314 D 1 H-012979 transcriptional coactivator14.08 23
PC4
M476 G4 H-013044 GA-binding protein 50.05 53.OkDa
transcription
factor, alpha subunit
(60kD)
M302 F3 H-013665 cathepsin O (GB:U13665)36.3 SO.OkDa
M311 G4 H-U 13831 cellular retinol binding14.85 20.OkDa
protein II
A2 H-013991 Human TATA-binding 24.09 34
protein
associated factor
30 kDa subunit
(tafII30) mRNA, complete
cds
M416 A4 H-U 14187 Human receptor tyrosine26.29 29.OkDa
kinase
ligand LERK-3 (EPLG3)
mRNA,
complete cds
M250 A2 H-U 14188 eph-related receptor 22.22 27
tyrosine
kinase ligand 4 [EPLG4]
M302 D2 H-014193 human TFIIA gamma 12.060 28.OkDa
subunit
mRNA
M416 G1 H-014603 Human protein-tyrosine18.48 30.OkDa
phosphatase (HU-PP-1)
mRNA,
partial sequence
E2 H-014747 Visinin-like 1 21.12 25
M266 D4 H-014966 ribosomal protein 32.78 38
LS
M314 E2 H-014967 ribosomal protein 17.71 29
L21
M266 FS H-014968 ribosomal protein 16.39 l9.OkDa
L27a
M248 E3 H-014969 ribosomal protein 15.18 27
L28
M266 El H-014971 ribosomal protein 21.45 30
S9
M250 C2 H-015009 small nuclear ribonucleoprotein,13.97 l7.OkDa
Sm D3
M311 D4 H-016660 enoyl-Coenzyme A hydratase-like36.19 38
protein, peroxisomal
M302 H4 H-017074 cyclin-dependent kinase18.59 29
6
inhibitor p 18
M306 A2 H-017195 A-kinase anchor protein72.05 100
100
[AKAP I 00* ]
Dl H-017280 Steroidogenic acute 31.46 35
regulatory
protein
M316 F1 H-018291 cell division cycle 68.2 7l.OkDa
protein 16
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CS H-U18420 Human ras-related 23.87 33
small GTP
binding protein RabS
(rab5)
mRNA, complete cds
M311 A2 H-U 18423 spinal muscular atrophy32.45 41
gene
M248 D4 H-U18914 hypothetical protein,20.35 32
(Human
19.8 kDa protein mRNA,
complete cds)
M302 BS H-U19718 microfibril-associated20.24 34.OkDa
glycoprotein 2
M305 E3 H-U20240 CCAAT/enhancer-binding16.61 29
protein
gamma
M302 A8 H-U20352 malate dehydrogenase 36.85 40
M416 F4 H-U20391 Human folate receptor28.38 34.OkDa
(FOLRI)
gene, complete cds
M311 D1 H-U20536 apoptotic cysteine 32.34 38.OkDa
protease Mch2
M431 G2 H-U20659 RNA polymerise II, 19.03 3l.OkDa
subunit B7
M499 C1 H-U20938 Human lymphocyte 112.86 100.0kDa
dihydropyrimidine
dehydrogenase
mRNA, complete cds
M305 F2 H-U20972 14-3-3 protein, epsilon28.16 36
M271 D3 H-U21049 _ 12.65 16
hypothetical protein
(GB:U2I049), ESTs,
Highly
similar to DD96 [H.sapiens].
M421 GS H-U21858 Human transcriptional29.15 38.OkDa
activation
factor TAFII32 mRNA,
complete
cds
M424 H3 H-U22662 Human nuclear orphan 49.28 49.OkDa
receptor
LXR-alpha mRNA, complete
cds
M271 D2 H-U24074 killer cell inhibitory37.62 43
receptor
[KIR], Homo Sapiens
natural
killer-associated
transcript 3
(NKAT3), complete
cds.
RECEPTOR ON NATURAL
KILLER (NK) CELLS
FOR
HLA-C ALLELES.
169-29 H-U24I53 human p21-activated 60 57.82
protein
kinase(Pak2) gene
M385 H2 H-U24166 EB1 29.59 36.OkDa
G1 H-U24169 Human 1TV-1 (JTV-1) 34.43 40
mRNA,
complete cds
E1 H-U24576 Human breast tumor 18.26 27
autoantigen
mRNA, complete sequence
G4 H-U24577 Human LDL-phospholipase48.62 52
A2
mRNA, complete cds
H1 H-U25789 Human ribosomal protein17.71 32
L2I
mRNA, complete cds
M416 DI H-U25849 Human red cell-type 17.49 28.OkDa
low
molecular weight acid
phosphatase (ACP 1
) gene, 5'
flanking region and
M300 A3 H-U26312 heterochromatin protein19.14 30
H-PIHs-
gamma
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M416 D3 H-026403 Human receptor tyrosine25.19 30.OkDa
kinase
ligand LERK-7 precursor
(EPLG7) mltNA, complete
cds
M317 E2 H-027143 human protein kinase 13.900 l7.OkDa
C inhibitor-
I cDNA
ES H-028249 Human l lkd protein 12.32 12
mltNA,
complete cds
F4 H-028386 Human nuclear localization58.3 54
sequence receptor
hSRPlalpha
mItNA, complete cds
M423 E3 H-028694 Chemokine (C-C) receptor39.16 39.OkDa
3
M266 G6 H-028963 Gps2 36.08 36
M306 D3 H-030610 CD94 antigen (NK/T-cell19.8 27
C-type
lectin receptor) [CD94]
B1 H-031116 Human beta-sarcogiycan35.09 33
A3b
mltNA, complete cds
M297 C2 H-031278 mitotic feedback control22.66 3l.OkDa
protein
Madp2 homolog
M302 G2 H-031384 guanine nucleotide-binding8.14 10
protein, gamma 11
subunit
F4 H-031986 Human cartilage-specific35.97 47
homeodomain protein
Cart-1
mIZNA, complete cds
M390 F3 H-032114 caveoiin 2 17.93 lB.OkDa
E4 H-032324 Human interleukin-11 46.53 54
receptor
alpha chain mItNA,
complete cds
F1 H-032576 Apolipoprotein C-IV 14.08 16
M298 C4 H-032907 p37NB protein 34.54 39
M300 D3 H-032944 dynein, light chain 9.9 15
1, cytoplasmic
M297 D1 H-032989 tryptophan 2,3-dioxygenase44.77 SO.OkDa
166-51 H-033052 "protein kinase PltK2110 108.3
[human,
DX3 B-cell myeloma
cell line,
mRNA]"
166-64 H-033054 "human G protein-coupled52 63.65
receptor kinase GRK4
mRNA,
alpha splice variant"
166-88 H-033055 "human G protein-coupled60 60.1
receptor kinase GltK4
mltNA,
beta splice variant"
166-76 H-033056 "human G protein-coupled58 58.59
receptor kinase GltK4
mIZNA,
gamma splice variant"
A2 H-034584 17.71 31
169-87 H-034820 human MAP kinase mRNA55 46.49
215-2 H-034822 human JNICI alpha2 55 47.04
protein
kinase (JNK1A2) mIZNA
169-37 H-035002 human JNK2 betal protein50 42.09
kinase
(JNK2B 1 ) mltNA
169-25 H-035003 human JNK2 beta2 protein55 46.71
kinase
(JNK2B2) mltNA
167-16 H-035004 human JNKl betal protein52 42.31
kinase
(JNK 1 B 1 ) mRNA
M300 B2 H-035048 TSC-22 protein 15.95 27
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M423 ES H-U35398 Human G protein-coupled40.26 48.OkDa
_ receptor mRNA, complete
cds
A3 H-U35735 Human RACH1 (RACH1) 42.9 78
mRNA, complete cds
M250 ES H-U36764 Eukaryotic translation35.86 36.OkDa
initiation
factor 3 (eIF-3) p36
subunit,
transforming gowth
factor-beta
receptor II interacting
protein 1
M270 E4 H-U37283 microfibril-associated19.14 32
glycoprotein-2 (GB:U37283)
M426 F3 H-U37352 Protein phosphatase 56.65 SS.OkDa
2A,
regulatory subunit
B' alpha-1
EI H-U37529 Human substance P 14.3 22
beta-PPT-A
mRNA, complete cds
M305 HS H-U37547 apoptosis inhibitor 68.09 64
M424 DS H-U38480 Human retinoid X receptor-51.04 61.OkDa
gamma mRNA, complete
cds
M270 F4 H-U38810 Human mab-21 cell
fate-
determining protein
homolog
(CAGRI) mRNA,
M467 F6 H-U38904 Human zinc finger 40.48 47.OkDa
protein C2H2-
25 mRNA, complete
cds
E2 H-U39318 Human E2 ubiquitin 16.28 22
conjugating
enzyme UbcHSC (UBCHSC)
mRNA, complete cds
166-75 H-U39657 human MAP kinase kinase40 36.81
6
(MKK6) mRNA
M298 E4 H-U39945 human adenylate kinase26.3633 38.OkDa
2 (adk2)
mRNA
166-38 H-U40282 human integrin-linked55 49.68
kinase
(ILK) mRNA
169-65 H-U40343 human CDK inhibitor 18 18. 33
pl9INK4d
mRNA
E2 H-U40705 Homo sapiens telomeric48.4 52
repeat
binding factor (TRF1)
mRNA,
complete cds
166-50 H-U40989 human tat interactive60 53.09
protein
mRNA
M266 H6 H-U41767 metargidin precursor 89.65 90
M270 F3 H-U41804 Human putative Tl/ST225.08 35.OkDa
receptor
binding protein precursor
mRNA,
complete cds
DS H-U42360 Human N33 gene 38.28 38
A1 H-U43368 Vascular endothelial 22.88 33
gowth
factor B
M421 G7 H-U43901 Human 37 kD laminin 32.56 58.OkDa
receptor
precursor/p40 ribosome
associated protein
gene, complete
cds
M392 C2 H-U43923 transcription factor 12.98 l6.OkDa
SUPTH4
E2 H-U46024 Myotubular myopathy 66.44 58
1
M330 A1 H-U46838 pI05MCM 90.42 97
M476 E2 H-U47677 Human transcription 48.18 53.OkDa
factor E2F1
(E2F 1 ) gene, promoter
and
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M421 H1 H-U48707 Human protein phosphatase-118.92 36.OkDa
inhibitor mltNA, complete
cds
_
M302 B7 H-U49070 peptidyl-prolyl isomerase18.04 28.OkDa
PINI
C1 H-U49188 Human placenta (Diff33)54.45 70
mltNA,
complete cds
M485 H2 H-U49837 Human LIM protein 21.45 34.OkDa
MLP mIZNA,
complete cds
D2 H-U49897 Homo sapiens phenylalanine49.83 64
hydroxylase (PAH)
mltNA,
complete cds
B2 H-U49957 Human LIM protein 67.43 67
(LPP)
mltNA, partial cds
166-16 H-U50196 human adenosine kinase50 38.02
mltNA
A4 H-U50939 Human amyloid precursor58.85 60
protein-binding protein
1 mltNA,
complete cds
G3 H-U51224 Human U2AFBPL gene, 52.8 55
complete
cds
M486 E3 H-U51333 Hexokinase 3 (white 101.64 100.OkDa
cell)
M305 D1 H-U51478 AT'Pase, Na+/K+transporting,30.8 36
beta 3 subunit
M416 H3 H-U52112 Homo Sapiens Xq28 25.96 36.OkDa
genomic
DNA in the region
of the L 1 CAM
locus containing the
genes for
neural cell adhesion
molecule LI
(L1CAM), arginine-vasopressin
receptor (A VPlt2),
C 1 p 115 (C 1 ),
ARDI N-acetyltransferase
related
protein (TE2), renin-binding
protein (RbP), host
cell factor 1
(HCF1}, and interleukin-1
receptor-associated
kinase
(IRAK) genes, complete
cds, and
Xq281u2 gene
M463 E1 H-U53442 human p38Beta MAP 40.99 49.OkDa
kinase
mltNA
G3 H-U53446 Human mitogen-responsive84.81 98
phosphoprotein DOC-2
mRNA,
complete cds
M463 C1 H-U54617 human pyruvate dehydrogenase45.28 52.OkDa
kinase isofonn 4 mltNA
169-38 H-U54645 methylmalonyl-coA 38 25.59
mutase
precursor
M300 H3 H-U56255 t-complex sterility 12.54 16
protein
homolog CW-1
C4 H-U56417 Human lysophosphatidic31.24 46
acid
acyltransferase-alpha
mltNA,
complete cds
M305 A2 H-U56637 actin-capping protein31.5? 31
alpha
subunit isoform 1
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M235 E6 H-056814 Human DNasel-Like 33.66 40.OkDa
III protein
(DNAS1L3) mRNA, complete
eds, involved in apoptosis
Binds
specifically to G-ACTIN
AND
BLOCKS ACTIN
POLYMERIZATION.
DS H-057059 31.02 36
-
B3 H-057093 Human small GTP-binding24.09 34
protein rab27b mRNA,
complete
cds
D3 H-057099 Human APEG-1 mRNA, 12.54 20
complete cds
F1 H-058331 Sarcoglycan, delta 28.27 24
(35kD
dystrophin-associated
glycoprotein)
M512 F4 H-058334 Human Bcl2, p53 binding110.66 108.OkDa
protein
Bbp/53BP2 (BBP/53BP2)
mRNA,
complete cds
B3 H-058516 Human breast epithelial42.68 50
antigen
BA46 mRNA, complete
cds
M250 E4 H-058522 Human huntingtin interacting22.11 30
protein (HIP2) mRNA,
complete
cds
M419 G2 H-060207 human stress responsive53.640 63.OkDa
serine/threonine protein
kinase
Krs-2 mRNA
M298 B2 H-060276 arsA homolog (hASNA-I)36.63 47.OkDa
B2 H-060521 Human protease proMch645.87 52
(Mch6)
mRNA, complete cds
F3 H-061166 Human SH3 domain-containing57.31 57
protein SH3P17 mRNA,
complete
cds
M250 BS H-061232 cofactor E (tubulin-folding
protein), REQUIRED
FOR
VIABILITY IN THE ABSENCE
OF THE KINESIN-RELATED
CINB
AS H-062392 Homo Sapiens zinc 43.45 52
finger protein
mRNA, complete cds
G1 H-062801 Human protease M mRNA,26.95 33
complete cds
M266 B 1 H-062962 Int-6 , Human Int-6 49.06 52.OkDa
mRNA,
complete cds
M300 G1 H-063295 seven in absentia 31.13 36
homolog
M306 H3 H-064198 94.93 98
H3 H-064863 Human hl'D-1 (hPD-1) 31.79 37
mRNA,
complete cds
B3 H-065581 Human ribosomal protein44.88 52
L3-like
mRNA, complete cds
M341 D1 H-065918 DAZ homologue [DAZLAJ32.56 36.OkDa
M302 E1 H-065928 Jun activation domain36.85 48.OkDa
binding
protein
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M512 D3 H-066347 Homo sapiens cAMP 46.97 60.OkDa
phosphodiesterase
(PDE4C)
mRNA, 4C-426 isoform,
complete cds
M306 F3 H-066867 ubiquitin-conjugating17.49 28
enzyme E2I
[UBE2I]
M416 E2 H-068111 Human protein phosphatase22.66 37.OkDa
inhibitor 2 (PPPllt2)
gene
F2 H-068382 Mannosidase, alpha 35.64 36
B, lysosomal
G2 H-069141 Glutaryl-Coenzyme 48.29 56
A
dehydrogenase
B2 H-070660 Human copper transport7.59 16
protein
HAH1 (HAH1) mRNA,
complete
cds
M297 B2 H-071374 peroxisomal membrane 40.15 40.OkDa
protein
(Pexl3p)
M306 A3 H-075272 progastricsin [PGC] 42.79 49.OkDa
A2 H-075285 Homo sapiens apoptosis15.73 25
inhibitor
survivin gene, complete
cds
B2 H-077456 Human nucleosome assembly41.36 50
protein 2 mltNA, complete
cds
C2 H-078294 Homo Sapiens 15S-lipoxygenase74.47 74
mRNA, complete cds
F6 H-078302 Human 2,4-dienoyl-CoA36.96 40
reductase gene
M478 G3 H-078798 Human TNF receptor 57.53 65.OkDa
associated
factor 6 (TRAF6) mltNA,
complete cds
G3 H-080982 Human myeloid-specific27.5 51
C/EBP-
epsilon transcription
factor
(CEBPE) gene, complete
cds
M468 B7 H-082256 Homo Sapiens arginase39.05 45.OkDa
type II
mItNA, complete cds
M465 B2 H-082812 Human scavenger receptor38.28 48.OkDa
cysteine rich Sp alpha
mIZNA,
complete cds
M484 D7 H-083410 Human CUL-2 (cul-2) 82.06 85.OkDa
mIZNA,
complete cds
M467 E6 H-083460 Human high-affinity 21.01 32.OkDa
copper
uptake protein (hCTRl)
mltNA,
complete cds
D2 H-084763 Homo Sapiens UCP3 34.43 42
mRNA,
complete cds
B2 H-086070 Homo sapiens 28.93 36
phosphomannomutase
mIZNA,
complete cds
C2 H-090441 Human prolyl 4-hydroxylase58.96 64
alpha (II) subunit
mRNA,
complete cds
B2 H-090543 Human butyrophilin 58.08 54
(BTF1)
mRNA, complete cds
H2 H-090545 Human sodium phosphate44.22 36
transporter (NPT4)
mRNA,
complete cds
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G2 H-090552 Human butyrophilin 56.54 48
(BTFS)
mRNA, complete cds
C3 H-091521 Peroxisomal biogenesis39.6 48
factor 12
H1 H-091641 Numan alpha2,8-sialyltransferase41.47 45
mRNA, complete cds
CI H-093869 Human RNA polymerase 34.98 36
III
subunit (RPC39) mRNA,
complete cds
F2 H-094346 Human calpain-like 70.4 65
protease
(tetra-3) mRNA, complete
cds
C2 H-094855 Human translation 39.38 36
initiation
factor 3 47 kDa subunit
mRNA,
complete cds
M271 F7 H-095089 Epidermal growth factor44.66 47
receptor.
M424 AS H-095847 Human GDNF receptor 50.71 52.OkDa
alpha
mRNA, complete cds
D2 H-096094 Human sarcolipin (SLN)3.52 10
mRNA,
complete cds
B3 H-096769 Homo Sapiens chondroadherin39.6 43
gene, S'flanking region
and
M298 G2 H-V00566 prolactin 25.08 35
M298 H2 H-V00571 corticotropin-releasing21.67 49
factor
217-61 H-V00572 phosphoglycerate kinase50 45.94
1
M314 B3 H-V00597 parathyroid hormone 12.76 14
M305 B8 H-X00129 retinol-binding protein22 51
4,
interstitial [RBP4]
F2 H-X00351 Human mRNA for beta-actin41.36 41
A4 H-X00570 apolipoprotein C-I 9.24 35
M362 E1 H-X01057 interleukin 2 receptor,30.03 40.OkDa
alpha
[IL2RA]
A4 H-X01677P Human liver mRNA for 10.45 10
glyceraldehyde-3-phosphate
dehydrogenase (G3PD,
EC
1.2.1.12)
M271 D6 H-X02152 lactate dehydrogenase36.63 45.0kDa
A [LDHA],
L-LACTATE
DEHYDROGENASE M CHAIN
A 1 H-X02158 Human gene for erythropoietin21.34 32
H4 H-X02415 Human gene for fibrinogen48.18 50
gamma chain
AS H-X02750 Protein C (inactivator50.82 53
of
coagulation factors
Va and VIIIa)
M302 B3 H-X02751 proto-oncogene N-ras 20.9 25.OkDa
D3 H-X02812 Human mRNA for transforming43.12 50
growth factor-beta
(TGF-beta)
M302 C H-X03124 tissue inhibitor of 22.88 36.OkDa
1
metalloproteinase
1
M362 B1 H-X03342 ribosomal protein 14.96 24.OkDa
L32
M235 A2 H-X03484 human mRNA for raf 71.350 73.OkDa
oncogene
M318 A3 H-X03557 interferon-induced 52.69 SO.OkDa
protein 56
A3 H-X03747 ATPase, Na+/K+ transporting,33.44 45
beta 1 polypeptide
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M30S D2 H-X04297 ATPase, Na+/K+ transporting,112.64 99
alpha subunit
M30S AS H-X04327 2,3-bisphosphoglycerate28.6 36
mutase
M271 GS H-X04S88 tropomyosin TM30nm, 26.29 40.OkDa
cytoskeletal
M30S C8 H-X04741 ubiquitin related 23.43 28.OkDa
protein
M236 AS H-XOS231 matrix metalloproteinaseS 1.7 S3.OkDa
1
(interstitial collagenase)
(MMPI),
CLEAVES COLLAGENS
166-S3 H-XOS246 "phosphoglycerate SO 45.94
kinase, testis
specific"
M236 AI H-XOS908 annexin I, REGULATES 38.17 40
PHOSPHOLIPASE A2
ACTIVITY, Binds CALCIUM
IONS
M2S0 A4 H-X06234 SI00 calcium-binding 10.34 lO.OkDa
protein A8
(calgranulin A)
M266 B6 H-X06323 ribosomal protein 38.39 39
L3, isoform 1
M313 A7 H-X06617 ribosomal protein 17.49 27
S11
M416 E4 H-X06948 High affinity IgE 28.38 36.OkDa
receptor alpha-
subunit (FcERI)
M421 H7 H-X07203 Human mRNA for CD20 32.78 40.OkDa
receptor
(S7)
217-2 H-X07743 pleckstrin 38 38.57
217-73 H-X07767 "CAMP-dependent protein4S 38.68
kinase,
alpha-catalytic subunit"
M30S B3 H-X07898 troponin C, skeletal,17.71 2S
fast
M306 E 1 H-X07979 integrin, beta 1 87.89 110
A11 H-X08004 ras-related protein 20.24 38
raplB
M23S A7 H-X12387 Cytochrome P4S0IIIA3 SS.44 60.OkDa
(nifedipine oxidase
chain 3)
M31 S F H-X 12496 glycophorin C 14. I 24
1 9
M316 D3 H-X 12S 17 small nuclear ribonucleoprotein17.6 30.OkDa
U1, C
M236 ES H-X12S34 guanine nucleotide-binding20.24 34.OkDa
protein rapt, ras-oncogene
related
M266 E3 H-X12S97 High-mobility group 23.76 37
(nonhistone
chromosomal) protein
1, placenta
217-14 H-X126S6 human mRNA for protein40 34.06
phosphatase 2A (beta
type)
H4 H-X12662 H.sapiens arginase 3S.S3 SO
gene exon 1
and flanking regions
(EC 3.5.3.1)
(and joined CDS)
C1 H-X129S3 RAB2, member RAS oncogene23.43 29
family
FS H-X139S6 Human 12S RNA induced9.13 19
by
poly(rI), poly(rC)
and Newcastle
disease virus
M297 A1 H-X1S005 laminin receptor 1 33.11 48.OkDa
M31S E3 H-X1S088 guanine nucleotide 38.61 4S
binding
protein (G protein),
alpha
transducing (transducin)
activity
polypeptide 1 [GNATIj
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G2 H-X 15183 Human mRNA for 90-kDa80.63 80
heat-
shock protein
M385 CI H-X15422 mannose-binding lectin,27.39 27.OkDa
soluble
(opsonic defect) [MBL]
M271 D7 H-X15606 INTERCELLULAR ADHESION30.36 37.OkDa
MOLECULE-2 PRECURSOR
[Homo sapiens].
M298 CS H-X15653 uracil-DNA glycosylase33.55 37
M302 B4 H-X15822 cytochrome-c oxidase,9.24 20
VIIa
subunit, liver
M305 A6 H-X15940 ribosomal protein 13.86 18
L31
M236 GS H-X 15949 interferon regulatory38.5 54.OkDa
factor 2 ,
BINDS AND REPRESSES
REGULATORY REGION
OF
TYPE I 1FN AND IFN-
INDUCIBLE MHC CLASS
I
GENES.
M236 C2 H-X16064 translationally-controlled19.03 35
tumor
protein
M512 BS H-X 16323 Hepatocyte growth 80.19 100.OkDa
factor
(hepapoietin A)
M3 I 5 H-X 16461 cell division cycle 32.78 40
C3 2, G 1 to S and
G2 to M [CDC2]
M297 G2 H-X 16832 cathepsin H 36.96 45.OkDa
M271 B H-X I 6983 integrin, alpha 4 114.29 114
1 (CD49D, alpha 4
subunit of VLA-4 receptor)
[ITGA4], IMPORTANT
FOR
CELL-CELL ADHESION
FUNCTION.
M270 A7 H-X17025 plasminogen activator-inducible25.19 34
c54, Human homolog
of yeast IPP
isomerase
M302 C3 H-X17042 proteoglycan 1, secretory17.49 26
granule
B 1 H-X 17206 ribosomal protein 24.42 45
S2
B4 H-X17254 Transcription factor 45.54 53
Eryfl
M311 H2 H-X17610 beta-1-glycoprotein, 46.97 48.OkDa
pregnancy-
specific (GB:X 17610)
M315 D1 H-X17644 G1 to S phase transition55 55
protein
(GSTI)
M340 G1 H-X51415 lipase, hormone-sensitive84.59 98.OkDa
[LIPE]
M464 A7 H-X51688 Cyclin A 47.63 47.OkDa
M313 G H-X51745 major histocompatibility40.26 50
1 complex,
class I, A
M297 A2 H-X51804 putative receptor 21.23 30
protein PMI
D4 H-X51952 Human UCP gene for 33.88 37
uncoupling
protein exons 1 and
2
M300 B1 H-X52011 muscle determining 26.73 39
factor
M419 G1 H-X52479 "protein kinase c, 82.28 85.OkDa
alpha type"
A2 H-X52486 Uracil-DNA giycosylase35.97 36
E3 H-X52520 Tyrosine aminotransferase50.05 58
B1 H-X52638 6-phosphofructo-2- 51.92 47
kinase/fructose-2,6-
bisphosphatase
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M509 C4 H-X52730 Human gene for 31.13 35.OkDa
phenylethanolamine
N-methylase
(PNMT) (EC 2.1.1.28)
M235 CS H-X52839 ribosomal protein 15.51 18
L17
M426 C2 H-X52943 Human mRNA for ATF-a53.24 64.OkDa
transcription factor
M266 GS H-X53777 ribosomal protein 20.35 31
L23
B4 H-X53961 Lactotransferrin 78.32 78
M462 C6 H-X54150 Fc fragment of IgA, 31.68 37.OkDa
receptor for
M302 A6 H-X54304 myosin, light polypeptide18.92 32.OkDa
2,
regulatory
M311 G2 H-X54802 cytochrome-c oxidase,18.7 23.OkDa
IV subunit
M270 H3 H-X54871 guanine nucleotide-binding23.76 33.OkDa
,
protein RabSB,ras-oncogene
related [RABSB],
PROTEIN
TRANSPORT. PROBABLY
INVOLVED IN VESICULAR
TRAFFIC (BYSIMILARITY).
M313 B6 H-X54936 placenta growth factor16.5 22.OkDa
[PLGF*]
M496 B2 H-X55079 Human lysosomal alpha-104.83 98.OkDa
glucosidase gene
exon 1
Dl H-X55330 Aspartylglucosaminidase38.17 36
EI H-X55448 H.sapiens G6PD gene 25.41 30
for
glucose-6-phosphate
dehydrogenase
M421 G6 H-X56253 Human MPR46 gene 30.58 52.OkDa
for 46kd
mannose 6-phosphate
receptor
169-89 H-X56468 14-3-3 protein tau 34 27.02
M300 B4 H-X56549 fatty-acid-binding 14.74 17
protein, muscle
M298 D2 H-X56740 guanine nucleotide-binding23.87 31.OkDa
protein rab 11 [RAB
11 *]
M266 ES H-X56932 highly basic protein,22.44 30.OkDa
23 kDa
M318 G1 H-X57025 insulin-like growth 16.94 18
factor I
M305 FS H-X57348 protein kinase C 27.39 35.OkDa
inhibitor
M236 D6 H-X57351 interferon-induced 14.63 24
protein 1-8D
H3 H-X57352 interferon-induced 14.74 38
protein I-8U
M305 B6 H-X58079 S-100 protein, alpha10.45 11
chain
E6 H-X59131 H.sapiens D13S106 34.76 50
mRNA for a
highly charged amino
acid
sequene
M248 HS H-X59268 transcription factor34.87 49
IIB [TCF2B*]
E2 H-X59357 Epstein-Barr virus 14.19 36
small RNA-
associated protein
M236 D4 H-X59417 macropain, iota subunit27.17 36
, THE
INTERACTION OF CALPONIN
WITH ACTIN INHIBITS
ACTOMYOSIN MG-ATPASE
ACTIVITY
M271 H4 H-X59618 ribonucleotide redactase,42.9 46
small
subunit
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M250 G3 H-X59710 CART-box DNA-binding 22.66 34
protein,
subunit B, CCAAT-BINDING
TRANSCRIPTION FACTOR
SUBUNIT A [Homo Sapiens]
M423 E2 H-X59711 Nuclear transcription38.28 48.OkDa
factor Y,
alpha
M271 C7 H-X59798 Cyclin D1 (PRADI; 32.56 40.OkDa
parathyroid
adenomatosis I). ESSENTIAL
FOR THE CONTROL OF
THE
CELL CYCLE AT THE
G 1 /S
(START) TRANSITION.
M270 HS H-X59834 calmodulin 41.14 53.OkDa
M416 DS H-X59871 Transcription factor 29.59 36.OkDa
7 (T-cell
specific)
M485 D6 H-X60036 Phosphate carrier, 39.82 37.OkDa
mitochondria)
M250 D4 H-X60489 translation elongation24.86 33.OkDa
factor 1,
beta
FS H-X60592 Human CDw40 mRNA for 30.58 46
nerve
growth factor receptor-related
B-
iymphocyte activation
molecule
M312 F3 H-X61587 ras-related rhoG 21.12 2l.OkDa
F9 H-X61622 cyclin-dependent kinase32.89 56
2
[CDK2]
M313 E3 H-X61970 macropain, zeta subunit26.62 35.OkDa
M428 D1 H-X62055 tyrosine phosphatase,65.?8 66.OkDa
non-
receptor type 6
M248 C4 H-X62534 high mobility group 23. I 37
protein 2,
BINDS PREFERENTIALLY
SINGLE-STRANDED DNA
AND UNWINDS DOUBLE
STRANDED DNA.
M305 F3 H-X62753 folate-binding protein28.38 36
M476 G2 H-X63468 H.sapiens mRNA for 48.4 53.OkDa
transcription
factor TFIIE alpha
G6 H-Xb3469 General transcription32.12 56
factor TFIIE
beta subunit, 34 kD
G4 H-X63522 Haapiens mRNA DAUDI6 58.74 54
for
retinoic acid X receptor
b
M316 G2 H-X63526 translation elongation48.18 52.OkDa
factor I,
gamma
M305 CS H-X63527 ribosomal protein 21.67 33
L19
E2 H-X63629 Cadherin 3 (P-cadherin)91.3 110
D4 H-X64037-2 General transcription56.98 64
factor IIF,
polypeptide I (74kD
subunit)
M302 C6 H-X64559 tetranectin 22.33 32.OkDa
M271 H 1 H-X64728 choroideremia-like 72.27 98
[CHML],
H.sapiens CHML mRNA
M270 E1 H-X64810 proprotein convertase82.94 90
subtilisin/kexin type
1 [PCSK 1 J,
INVOLVED IN PROCESSING
OF HORMONE AND OTHER
PROTEIN PRECURSORS
M31 I F4 H-X64877 complement factor 29.81 36.OkDa
H-related
protein
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M388 Dl H-X65293 protein kinase C, 81.18 96.OkDa
epsilon
[PRKCE]
BS H-X65873 kinesin, heavy polypeptide106.04 34
F4 H-X66079 Spi-B transcription 28.93 54
factor (Spi-
I/PU.I related)
F3 H-X66114 2-oxoglutarate carrier0 37
protein
[OGMT*]
M305 C6 H-X66141 myosin, light polypeptide18.37 31
2,
regulatory, ventricular
M419 H1 H-X66357 cell division protein33.620 44.OkDa
kinase 3
166-13 H-X66358 serine/threonine-protein45 39.45
kinase
KKIALRE
166-25 H-X66360 serine/threonine-protein60 57.60
kinase
PCTAIRE-2
M419 A2 H-X66363 serine/threonine-protein54.600 64.OkDa
kinase
PCTAIRE-1
166-37 H-X66364 H.sapiens mRNA PSSALRE38 32.19
for
serine/threonine protein
kinase
M419 B2 H-X66365 cell division protein35.900 46.OkDa
kinase 6
H3 H-X66839 H.sapiens MaTu MN 50.6 54
mRNA for
p54/58N protein
M266 G3 H-X67325 interferon, alpha-inducible13.53 13
gene
p27
M462 H7 H-X67594 Melanocortin I receptor34.98 44.OkDa
(alpha
melanocyte stimulating
hormone
receptor)
M236 CS H-X67951 Proliferation-associated22 34
gene A
(natural killer-enhancing
factor
A), PAGA
H3 H-X68486 Adenosine receptor 45.43 45
A2
M429 E3 H-X68561 Sp4 transcription 86.35 86.OkDa
factor
M430 F2 H-X69151 ATP synthase, H+ transporting,42.13 58.OkDa
subunit C, vacuolar
M236 C3 H-X69392 ribosomal protein 16.06 29
L26
B3 H-X69532 H.sapiens gene for 100.32 98
inter-alpha-
trypsin inhibitor
heavy chain Hl,
exons I-3
M236 FS H-X69654 ribosomal protein 12.76 18
S26
M421 C8 H-X70218 Protein phosphatase 33.88
4 (formerly
X), catalytic subunit
M266 HS H-X70848 protein phosphatase 36.41 37
1, alpha
catalytic subunit
E1 H-X70940 Eukaryotic translation51.04 60
elongation
factor 1 alpha 2
M270 F1 H-X72215 [PITT], POU domain, 32.12 40.OkDa
class 1,
transcription factor
1 (Pit 1,
growth hormone factor
1 )
M271 A7 H-X72760 Laminin, beta 2 (laminin67.87 75.OkDa
S), S-
LAMININ IS A LAM1NIN-LIKE
ADHESIVE PROTEIN
CONCENTRATED IN THE
SYNAPTIC CLEFT OF
THE
NEUROMUSCULAR
JUNCTION.
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M235 B H-X72841 Human retinoblastoma-binding46.86 52.OkDa
1
protein (RbAp46) mRNA,
complete cds, IEF
7442
(GB:X72841)
217-25 H-X73428 DNA-binding protein 20 17.08
inhibitor
ID-3
-
M305 BS H-X73459 signal recognition 15.07 20
particle,
subunit 14
M250 D6 H-X73460 ribosomal protein 44.44 SO.OkDa
L3, isoform 2,
COMPONENT OF THE LARGE
SUBUNIT OF CYTOPLASMIC
RIBOSOMES
M462 D8 H-X74008 Protein phosphatase 35.64 46.OkDa
1, catalytic
subunit, gamma isofonm
M266 G2 H-X74104 Signal sequence receptor,20.24 27
beta;
translocon-associated
protein,
beta subunit
M266 E7 H-X74262 retinoblastoma binding46.86 SO.OkDa
protein
RbAp48
H1 H-X74330 DNA primase polypeptide46.31 51
I
(49kD)
M313 F3 H-X74570 gal beta (1-3/1-4) 36.3 46.OkDa
GIcNAc alpha-
2,3 sialyltransferase
(GB:X74570)
M429 H3 H-X74764 Iiaapiens mRNA for 94.120 98.OkDa
receptor
protein tyrosine kinase
M271 E6 H-X75042 V-rel avian reticuloendotheliosis68.2 88
viral oncogene homolog
M305 G2 H-X75252 phosphatidylethanolamine-20.68 30
binding protein
M302 Gi H-X75593 guanine nucleotide-binding22.44 32.OkDa
protein rabl3
166-49 H-X75958 H.sapiens trkB mRNA 55 52.54
for
protein-tyrosine kinase
C4 H-X76013 Haapiens QRSHs mRNA 85.36 85
for
glutaminyl-tRNA synthetase
A2 H-X76029 H.sapiens mRNA for 19.25 20
neuromedin
U
M305 DS H-X76228 ATP synthase, H+ transporting,24.97 36
subunit E, vacuolar
M298 F6 H-X76648 glutaredoxin 11.77 ll.OkDa
M311 A4 H-X76717 metallothionein 11 6.82 14
C4 H-X77533 H.sapiens mRNA for 56.43 61
activin type
IIreceptor
H2 H-X77548 H. sapiens cDNA for 67.65 67
RFG
169-41 H-X77743 Haapiens CDK activating45 38.1
kinase 3
mRNA
A4 H-X77909 H.sapiens IKBL mRNA 42.02 52
M305 C1 H-X78136 heterogeneous nuclear40.26 40.OkDa
ribonucleoprotein
E2
M306 G2 H-X78416 casein, alpha [CSN 20.46 33
1 ]
M271 C2 H-X78678 ketohexokinase (fructokinase)32.89 39
[KHK], H.sapiens KHK
mRNA
for ketohexokinase,
clone
pHKHK3a
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M305 D4 H-X79193 cyclin-dependent kinase38.17 35
7
(homolog of Xenopus
MO15 cdk-
activating kinase)
[CDK7]
M431 F2 H-X79389 glutathione S-transferase26.51 34.OkDa
Tl
M298 C6 H-X79537 glycogenin 30.8 34.OkDa
M440 C1 H-X79865 H.sapiens Mrpl7 mRNA 21.89 3l.OkDa
M298 FS H-X80229 protein kinase PKN 52.8 64.OkDa
167-39 H-X80230 H.sapiens mRNA (clone42 40.99
C-2k)
mRNA for serine/threonine
protein kinase
217-49 H-X80343 H.sapiens p35 mRNA 40 33.84
for
regulatory subunit
of cdk5 kinase
M270 D7 H-X80695 cytochrome oxidase-assembly47.96 50
protein, OXA1, H.sapiens
OXA 1 Hs mRNA
M266 BS H-X80909 nascent polypeptide-associate23.76 37.OkDa
complex, alpha
M416 D9 H-X80910 Protein phosphatase 36.08 45.OkDa
1, catalytic
subunit, beta isofonm
E2 H-X81198 Archain 52.03 63
169-6 H-X81817 H.sapiens BAP31 mRNA 32 27.1
3
E4 H-X82018 H.sapiens mRNA for 46.75 57
ZID protein
M313 D7 H-X82456 MLN50 28.82 33
A2 H-X82629 H.sapiens mRNA for 33.44 42
Mox-2
M236 D1 H-X83006 lipocalin, neutrophil21.89 34.OkDa
gelatinase
associated
166-40 H-X83107 H.sapiens Bmx mRNA ~ 75 74.32
for
cytoplasmic tyrosine
kinase
E3 H-X83425 H.sapiens LU gene 69.19 59
for Lutheran
blood group glycoprotein
C6 H-X83703 H.sapiens mRNA for 35.2 54
cytokine
inducible nuclear
protein
M416 H2 H-X83928 H.sapiens mRNA for 23.32 33.OkDa
transcription
factor TFIID subunit
TAFII28
166-17 H-X85106 H.sapiens mRNA for 90 80.70
ribosomal
S6 kinase
166-39 H-X85337 H.sapiens mRNA for 110 109.0
myosin light
chain kinase
D2 H-X85750 H.sapiens mRNA for 26.29 30
transcript
associated with monocyte
to
macrophage differentiation
M266 E6 H-X87176 17-beta-hydroxysteroid81.07 65
dehydrogenase,type
4
M297 F2 H-X87689 CLCP 23.21 33.OkDa
M300 A2 H-X87843 cyclin H assembly 34.1 47
factor
M271 E3 H-X89750 homeotic protein, 30.03 32.OkDa
TGIF,
H.sapiens mRNA for
TGIF
protein
M235 GI H-X90529 guanine nucleotide-binding34.54 40
protein ragA [RAGA)
M302 E6 H-X90583 translocon-associated19.14 28.OkDa
protein,
delta
M306 G1 H-X90872 gp2512 23.65 33
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M416 D2 H-X91504 Transcription factor 22.22 32.OkDa
COUP 2
(a.k.a. ARP1)
M250 B3 H-X92098 transmembrane protein22.22 30
rnp24
M271 G7 H-X92106 bleomycin hydrolase. 50.16 SS.OkDa
PROTECTING NORMAL
AND
MALIGNANT CELLS FROM
BLM TOXICITY.
F3 H-X92715 Zinc finger protein 63.03 47
74 (Cos52)
M270 H6 H-X92720 H.sapiens mRNA for 70.51 71
phosphoenolpyruvate
carboxykinase
HS H-X92762 H.sapiens mRNA for 32.23 37
tafazans
protein
M298 D3 H-X93036 MAT-8 9.68 l6.OkDa
M476 AS H-X93595 H.sapiens mRNA for 50.16 56.OkDa
NK receptor
(clone 17. I C)
M417 D2 H-X93920 protein tyrosine phosphatase41.980 48.OkDa
foreskin
AS H-X95592 H.sapiens mRNA for 15.62 28
CID protein
M298 B4 H-X95648 translation initiation33.66 34.OkDa
factor 2B,
alpha subunit
F3 H-X95735 H.sapiens mRNA for 63.03 72
zyxin 2
M386 B1 H-X96752 L-3-hydroxyacyl-CoA 34.65 45.OkDa
dehydrogenase, SCHAD
gene
M422 B6 H-X97229 H.sapiens mRNA for 41.58 48.OkDa
NK
receptor, clone library
15.212
B3 H-X98173 H.sapiens mRNA for 51.15 51
MACH-
alpha-2 protein
166-14 H-X99325 H.sapiens mRNA for 55 46.9
Ste20-like 3
kinase
C4 H-X99459 H.sapiens mRNA for 21.34 30
sigma 3B
protein
M424 C4 H-Y00291 Human hap mRNA encoding49.39 59.OkDa
a
DNA-binding hormone
receptor
M386 H1 H-Y00345 polyadenylate-binding69.74 70.OkDa
protein
M469 A2 H-Y00630 Plasminogen activator45.76 46.OkDa
inhibitor,
type II (arginine-serpin)
M305 E1 H-Y00711 lactate dehydrogenase36.85 38.OkDa
B
H2 H-Y00764 ubiquinol/cytochrome 10.12 33
c reductase
hinge protein
FS H-Y07848 Haapiens EWS, gar22, 36.3 50
rrp22 and
bam22 genes
M305 G6 H-211559 iron-responsive element97.9 98
binding
protein 1 [IREB I
]
M250 F3 H-211566 Pr22 protein , STATHMIN16.5 22.OkDa
[Homo sapiens], SERVES
AS
RELAY (VIA
PHOSPHORYLATION) FOR
DIVERSE SECOND
MESSENGER PATHWAYS
169-73 H-211695 H.sapiens 40 kDa protein50 38.35
kinase
related to rat ERK2
M475 C8 H-211737 Flavin-containing 61.49 70.OkDa
monooxygenase 4
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Ci H-211898 Octamer binding protein39.71 50
3
M266 H4 H-212830 SSR, alpha subunit 31.57 42.OkDa
A3 H-214000 Ring finger protein 41.58 50
1
M300 E1 H-214978 actin-related protein41.47 49
G 1 H-Z 19002 H.sapiens of PLZF 74.14 84
gene encoding
kruppel-like zinc
forger protein
H 1 H-221966 POU homeobox protein 33.22 43
M248 G3 H-223139 CLASS II 29.04 34
HISTOCOMPATIBILITY
ANTIGEN, M BETA CHAIN
PRECURSOR [Homo sapiens]
D3 H-226876 ribosomal protein 7.81 35
L38
F2 H-228339 H.sapiens mRNA for 35.97 43
delta 4-3-
oxosteroid 5 beta-reductase
M298 B3 H-228407 ribosomal protein 28.38 39.OkDa
L8
M313 C3 H-229330 ubiquitin-conjugating20.24 34
enzyme
UbcH2, 23 kDa
M271 F3 H-229677 guanine nucleotide-binding20.35 28.OkDa
protein, ras-related
M465 C2 H-230425 H.sapiens mRNA for 38.39 34.OkDa
orphan
nuclear hormone receptor
M302 FS H-231357 cysteine dioxygenase 22.11 3l.OkDa
M340 C1 H-231695 inositol polyphosphate40.04 49.OkDa
5-
phosphatase, 43 kDa
E3 H-232564-2 H.sapiens FRGAMMA 26.84 36
mRNA
(819bp) for folate
receptor
M236 H1 H-235227 small G protein, TTF,21.12 30.OkDa
RAS-
RELATED PROTEIN RAC
1
A10 H-235491 H.sapiens mRNA for 30.25 60
novel
glucocorticoid receptor-associated
protein
M440 GS H-237986 H.sapiens mRNA for 25.41 28.OkDa
phenylalkylamine binding
protein
M297 E2 H-247087 cyclin A/cyclin-dependent18.04 30.OkDa
kinase
2-associated p19
F1 H-248051 H.sapiens gene for 27.28 31
myelin
oligodendrocyte glycoprotein
(MOG)
A2 H-248475 Glucokinase regulator68.86 70
M302 E4 H-248570 sperm zona pellucida-binding16.72 24
protein
M266 A2 H-268907 Human clone ID 19322543.34 45.OkDa
NAD
(H~specific isocitrate
dehydrogenase gamma
subunit
mRNA, alternatively
spliced,
partial cds
G1 H-283850 Human DNA sequence 45.76 60
from PAC
82J I 1 and cosmid
U 134E6 on
chromosome Xq22. Contains
NIK
like and Thyroxin-binding
globulin precursor
(T4-binding
globulin, TBG) genes,
ESTs and
STSs
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H4 H-297171 Homo sapiens GLCIA 55.55 55
(trabecular
meshwork induced glucocortcoid
response) gene, exon
I, joined
CDS
M421 DS H-297632 Human DNA sequence 28.49 38.OkDa
from PAC
196E23 on chromosome
Xq26.1-
27.2. Contains the
TAT-SF1
(HIV-1 transcriptional
elongation
factor TAT cofactor
TAT-SFI)
gene, the BRS3 (Bombesin
Receptor subtype-3
(Uterine
Bombesin Receptor,
BRS-3)
gene, an unknown gene
coding
for two isoforms,
a predicted CpG
island, ESTs and STSs
The following example illustrates the construction of the expression vectors
5 used in the Examples above. Similar modifications can be made in other
vectors for
use in creating libraries of expressible gene sequences.
The vector pcDNA3.1/VS-His was obtained from Invitrogen (cat #V810-20)
and modified slightly so that it carried an gene sequence for ZeocinTM
resistance and
lacked the multiple cloning site. A 100~.g aliquot was suspended in 200 ~tl
medical
10 irrigation (MI) water. A Sp,l aliquot was saved for gel analysis. The
remainder was
transferred to a 1.7 ml Eppendorf tube. The vector was digested with HindIII
(400 U)
using Promega Buffer E (final volume = 400 ~l). The reaction ran 3 hours at
37° C.
An aliquot was checked for completeness of digestion by running on an 0.8%
agarose
gel in 1 X TAE, and visualizing with ethidium bromide.
15 The digested vector was treated with 200 p,l phenol/chloroform (pH7.5)
according to standard procedures, and the DNA precipitated from the aqueous
phase
using 1/10 volume 3M NaOAc and 2 volumes 100% EtOH at room temperature,
followed by washing with 80% EtOH. The pellet was resuspended in 100 ul MI
water.
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Two oligonucleotides were added to the resuspended DNA (Topo -H (40 pg)
5'-(P)AGCTCGCCCTTATTCCGATAGTG (SEQ ID N0:3), Topo-4 (12 g.g) 5'-
(P)AGGGCG (SEQ ID N0:4)), plus I7 ~,1 l OX Promega T4 Ligase buffer. The tube
was placed on ice and the volume increased to 170 Itl with MI water. The
oligos were
S ligated to the vector using 20U Promega T4 DNA ligase, incubated at
I2° C
overnight.
The vector was treated with 100 ~1 phenoUchloroform and the aqueous phase
precipitated as described above. The pelleted DNA was resuspended in 150 p.l
of
sterile water the redigested with HindIII (17 ~,1 Promega Buffer E, 200 U
HindIII - 37°
C, 1 hour). The redigested DNA was re-extracted with phenol/chloroform and
precipitated with 1/10 volume 3M NaOAc and 7/10 volume isopropanol, then
washed
with 80% EtOH.
The pelleted DNA was resuspended in 82 ~.1 TE buffer ( 10 mM Tris, pH 8.0, 1
mM EDTA, pH 8.0). A 2 ~,1 aliquot was used to check the foregoing procedure
using
agarose gel electrophoresis as described above. The remaining 80 ~l was
transferred
to a Falcon tube and mixed with 16 ~g Topo-5 oligonucleotide (S'-
(P)CAACACTATCGGAATA (SEQ ID NO:S). To this mixture was added 190 ~,1
NEB Restriction Buffer # 1 (room temperature). The total reaction mixture was
adjusted to 1.9 mls with MI wafer. Vaccinia Topoisomerase I enzyme was added
(80
fig) and the reaction tube placed in a 37° C water bath for 15 minutes.
After 15 minutes, 200 ~,1 of room temperature Topo-lOX stop buffer was
added (100 mM Tris 7.4, 110 mM EDTA, bromophenol blue). The entire volume was
loaded onto an agarose gel ( 1.2 gr agarose/ 130 mls 1 X TAE) and run at 70
volts until
the bromophenol blue dye had run down about I /2 in (volume in the loading
well was
kept constant by the addition of 1 X TE). The voltage was reversed for 90
seconds.
The contents of the loading well were transferred to a 15 ml Falcon tube and
placed
on ice. 2 mls of cold Topo-2X Wash Buffer (60 mM Tris 7.4, 1 mM EDTA, 4 mM
dithiothreitol (DTT), 200 ltg/ml bovine serum albumin (BSA)) was added and the
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volume then adjusted to 4 mls with cold Topo-1X Enzyme Dilution Buffer (50%
glycerol, 50 mM Tris 7.4, 1 mM EDTA, 2 mM DTT, 0.1 % Triton X-100, 100 pg/ml
BSA) plus 4 mls Topo-Glycerol mix (90% glycerol, 10% 50 mM TE pH 7.4, 0.1%
Triton X-100) and stored until needed.
A similar procedure was used to make Topo-adapted pYES2 (Invitrogen cat #
V 825-20).
While the foregoing has been presented with reference to particular
embodiments of the invention, it will be appreciated by those skilled in the
art that
changes in these embodiments may be made without departing from the principles
and
spirit of the invention, the scope of which is defined by the appended claims.
