Note : Les descriptions sont présentées dans la langue officielle dans laquelle elles ont été soumises.
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PHARMACEUTICAL COMPOSITIONS COMPRISING HYDROXYLANSOPRAZOLE AND USES THEREOF
FIELD OF THE INVENTION
This invention relates to compositions of matter containing
hydroxylansoprazole.
The invention also relates to methods of treating and preventing ulcers,
treating other
conditions related to gastric hypersecretion, and treating psoriasis.
BACKGROUND OF THE INVENTION
Lansoprazole I is an orally active, potent,
H
N
N / H3C OCH2CF3
~-P/
I
irreversible inhibitor of H,K-ATPase. It is commercially available in the form
of
Prevacid delayed release capsules from TAP Pharmaceuticals Inc. The compound
is one
of the class of compounds known as gastric "proton pump" inhibitors. These
compounds
are weak organic bases which diffuse passively from the plasma into the acid-
containing
intracellular canaliculi of gastric parietal cells. At the low pH found in the
lumen of these
canaliculi, the protonated compounds rearrange to form pyridinium
sulfenamides, which
react with sulfliydryl groups present on the ATPase localized in the membranes
lining the
intracellular canaliculi. The alkylation of the sulfhydryl inhibits the
ability of the enzyme to
catalyze the secretion of H+ into the lumen in exchange for K+ ions. This
inhibition results
in an overall reduction in hydrochloric acid secretion by the parietal cells
into the cavity of
the stomach, thus increasing intragastric pH. As a consequence of reduced
acidity in the
stomach, the activity of the proteolytic enzyme pepsin is also markedly
decreased. Because
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the proton pump is the fnal step in acid production and the compounds of this
class
combine covalently with the associated H,K-ATPase, a profound and prolonged
inhibition
of gastric acid secretion can be achieved.
Proton pump inhibitors have also been reported as useful in treating
psoriasis. [See
PCT application W095/18612]
The C. of racemic lansoprazole is at about 1.5 to 3.5 hours in humans after
administration of enteric-coated granules, and the serum half-life is about 1
to 3 hours,
although this is variable, depending on the subject's age and liver function,
as discussed
below. The major metabolites in human serum are 5-hydroxylansoprazole II
(referred to as
hydroxylansoprazole herein)and lansoprazole sulfone III.
H
N
I N
HO N
H3C OCH2CF3
II
N /O
I / S N
N
H3C OCH2CF3
III
The two major primary metabolites, lansoprazole sulfone and 5-
hydroxylansoprazole, are formed by cytochromes P450 3A (CYP3A) and 2C 19
(CYP2C 19), respectively. Both metabolites undergo further metabolism to the
common
metabolite 5-hydroxylansoprazole sulfone via CYP2C19 and CYP3A, respectively.
Thus,
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both CYP enzymes are sequentially-but alternatively--invoived in lansoprazole
metabolism.
CYP2C 19, the S-mephenytoin hydroxylase, is polymorphically expressed in the
human
population. The mutant allele constitutes the recessive trait. Homozygous
carriers of the
mutation completely lack CYP2C 19 and are referred to as poor metabolizers
(PM's);
persons homozygous and heterozygous for the "nonmal" allele are extensive
metabolizers
(EM's). A hereditary deficiency of the alternative enzyme, CYP3A, has not been
demonstrated in the human population.
It would be desirable to find a compound with the advantages of lansoprazole
which
would provide a more predictable dosage regimen in the patient population and
that would
decrease the chances for drug-drug interactions.
SUMMARY OF THE INVENTION
This invention relates to the use of hydroxylansoprazole for treating ulcers
of the
stomach, duodenum and esophagus, gastroesophageal reflux diseases, Zollinger-
Ellison
Syndrome, and other disorders including those that would benefit from an
inhibitory action
on gastric acid secretion. Hydroxylansoprazole inhibits the W, K+-ATPase
associated with
the gastric proton pump and the resulting secretion of gastric acid by
parietal cells providing
therapy in diseases associated with gastric hyperacidity. The invention also
relates to a
method of treating psoriasis using hydroxylansoprazole. Hydroxylansoprazole
provides a
more predictable dosage regimen in the patient population and decreases the
chances for
drug-drug interactions by avoiding oxidative metabolism for which the
cytochrome P450
2C 19 enzyme system is required.
The invention also relates to certain pharmaceutical compositions containing
hydroxylansoprazole.
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DETAILED DESCRIPTION OF THE INVENTION
i
The active compound of these eompositions and methods is hydroxylansoprazole.
Racemic hydroxylansoprazole may be prepared using methods well larnown to
those skilled
in the art, e.g. by a modification of the method described in United States
Patents
4,628,098 and 4,689,333. The compound identified in these patents as
intermediate II
Ri
N
~SH
N
H
is provided with a substituent R' = methoxymethyl (MOM) by treatment of the
corresponding hydroxy compound with dimethoxymethane (formaldehyde dimethyl
acetal)
in the presence of an acid catalyst (e.g. TsOH) and molecular sieves.
Following
condensation with the hydrohymethylpyridine III of the patents, and before
oxidation, the
MOM protecting group is cleaved with HCI in isopropanol/THF. Throughout this
application, various references are referred to, often, although not always,
within
parentheses or square brackets.
The index name for hydroxylansoprazole (II) in Chemical Abstracts is 2-[[[3-
methyl-4-(2,2,2-trifluoroethoxy)-2-pyridinyl]methyl]sulfinyl]-1H-benzimidazol-
5-ol; it is
also known as 5-hydroxy-2-[3-methyl-4-(2,2,2-trifluoroethoxy)pyrid-2-yl)methyl-
sulfinylbenzimidazole. The registry number of racemic hydroxylansoprazole is
131926-
998-2.
Hydroxylansoprazole possesses a center of asymmetry at the sulfoxide sulfur,
giving rise to two enantiomers. Throughout the instant disclosure, when the
term is not
otherwise modified, hydroxylansoprazole includes the (+) enantiomer, the (-)
enantiomer and
any mixture of the two. The preparation of the individual enantiomers of the
parent,
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lansoprazole, has been described in the literature, but the enantiomers of
hydroxylansoprazole have not been previously disclosed. The individual
enantiomers of
hydroxylansoprazole can be obtained using methods well known to those skilled
in the art,
e.g. by asymmetric oxidation of the thioether precursor, or by achiral
oxidation of the
thioether precursor followed by separation of the enantiomers, e.g. by
bioreduction of the
racemate to eliminate one or the other enantiomer in analogous fashion to the
procedure
described for lansoprazole in PCT applications WO 9602535 and 9617077.
Inatomi et al. [Yakuri to Chiryo 19, 477-486 (1991); Chem. Abst. 115:21996]
have
indicated that although the parent lansoprazole inhibited acid formation in
isolated parietal
cells with an IC50 of 0.09 M, racemic hydroxylansoprazole did not inhibit
acid formation
in isolated parietal cells.
It has now been discovered that hydroxylansoprazole is a superior agent for
treating
ulcers of the stomach, duodenum and esophagus, gastroesophageal reflux
diseases,
Zollinger-Ellison Syndrome, psoriasis and other disorders, including those
that would
benefit from an inhibitory action on H+,K-ATPase in that it provides this
effective
treatment while exhibiting fewer or less severe adverse effects than
lansoprazole, less
potential for drug-drug interactions than lansoprazole and a more predictable
dosing
regimen than lansoprazole. Adverse effects of lansoprazole include
hepatocellular
neoplasia, gastric carcinoids, headache, diarrhea and skin alterations.
The present invention encompasses a method of treating ulcers, which comprises
administering to a human in need of such therapy, an amount of
hydroxylansoprazole, or a
pharmaceutically acceptable salt thereof, sufficient to alleviate the symptoms
of ulcers. The
present invention also encompasses an oral antiulcer composition for the
treatment of a
human in need of antiulcer therapy, which comprises a pharmaceutically
acceptable carrier
for oral administration and a therapeutically effective amount of
hydroxylansoprazole or a
pharmaceutically acceptable salt thereof. Preferably the composition is in the
form of a
tablet or capsule, and the amount of hydroxylansoprazole in the tablet or
capsule is
preferably about between 100 and 500 mg. The present invention further
encompasses a
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method of treating gastroesophageal reflux disease and of treating conditions
caused by or
contributed to by gastric hypersecretion. Conditions associated with
hypersecretion in
humans may include, but are not limited to, Zollinger-Ellison syndrome. The
present
invention further encompasses a method of treating psoriasis.
Utilizing hydroxylansoprazole results in enhanced dosage predictability and an
improved therapeutic index. In particular, hydroxylansoprazole exhibits less
variation in the
patient population between so-called extensive metabolizers and poor
metabolizers than
does lansoprazole.
The term "treating ulcers" as used herein means treating, alleviating or
palliating
such conditions, and thus providing relief from the symptoms of nausea,
heartburn, post-
prandial pain, vomiting, and diarrhea. The term "a method for treating
gastroesophageal
reflux diseases in a human" as used herein means treating, alleviating or
palliating the
conditions that result from the backward flow of the stomach contents into the
esophagus.
The term "treating a condition caused, or contributed to, by gastric
hypersecretion in a
human" as used herein means treating, alleviating or palliating such disorders
associated
with hypersecretion, thus providing relief from the symptoms of the
aforementioned
conditions. Zollinger-Ellison Syndrome is among the conditions caused by or
contributed to
by hypersecretion. The term "treating psoriasis" as used herein means
treating, alleviating
or palliating the condition, and thus providing relief from the symptoms of
pruritis,
epidenmal scaling, itching and burning.
The term "optically pure" as used herein means that the compositions contain
at
least 90% by weight of one enantiomer and 10% by weight or less of the other.
In a more
preferred embodiment the term "substantially optically pure" means that the
composition
contains at least 99% by weight of one enantiomer, and 1% or less of the
opposite
enantiomer. In the most preferred embodiment, the term "substantially
optically pure" as
used herein means that the composition contains greater than 99% by weight of
a single
enantiomer. These percentages are based upon the total amount of
hydroxyomeprazole in
the composition.
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The magnitude of a prophylactic or therapeutic dose of hydroxylansoprazole in
the
acute or chronic management of disease will vary with the severity of the
condition to be
treated and the route of administration. The dose and perhaps the dose
frequency will also
vary according to the age, body weight and response of the individual patient.
In general,
the total daily dose range for hydroxylansoprazole for the conditions
described herein is
from about 50 mg to about 1500 mg in single or divided doses. Preferably a
daily dose
range should be about 500 mg to about 1000 mg in single or divided doses. In
managing
the patient, the therapy should be initiated at a lower dose, perhaps at about
50 mg and
increased up to about 1000 mg or higher depending on the patient's global
response. It is
further recommended that children and patients over 65 years and those with
impaired renal
or hepatic function, initially receive low doses, and that they be titrated
based on individual
response(s) and blood level(s). It may be necessary to use dosages outside
these ranges in
some cases as will be apparent to those skilled in the art. Further, it is
noted that the
clinician or treating physician will know how and when to interrupt, adjust,
or tenminate
therapy in conjunction with individual patient response. The terms "an amount
sufficient to
alleviate or palliate ulcers" "an amount sufficient to alleviate the symptoms
of
gastroesophageal reflux", "an amount sufficient to alleviate gastric
hypersecretion" and "an
amount sufficient to treat psoriasis" are encompassed by the above-described
dosage
amounts and dose frequency schedule.
The relative activity, potency and specificity of hydroxylansoprazole both as
a
gastric antisecretory agent and as a plasma gastrin elevating agent can be
determined by a
pharmacological study in animals according to the method of Decktor et al. [L
Pharmacol.
Exp. Ther. 249, 1-5 (1989)]. The test provides an estimate of relative
activity, potency and,
through a measure of specificity, an estimate of therapeutic index. Fasted
rats, implanted
with a gastric cannula, receive single oral or parenteral doses of (+)
hydroxylansoprazole, (-
) hydroxylansoprazole or racemate, 1 hour before collection of gastric juice
over a four hour
period. Acid output and pH are then determined on each sample. Dose response
evaluations are performed with each compound to determine the lowest dose
which inhibits
acid output by at least 95% and maintains gastric pH above 7Ø Plasma gastrin
levels are
then determined in a second group of rats treated with the doses selected in
the first series of
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tests. Blood samples are taken for analyses over the five hour period after
dosing, and both
peak level as well as area-under-the-curve analyses of the gastrin responses
are made.
These responses are then analyzed statistically using Student's "t" test to
assess whether
equivalent antisecretory doses show differences in gastrin responses.
Any suitable route of administration may be employed for providing the patient
with an effective dosage of hydroxylansoprazole. Rectal, parenteral
(subcutaneous,
intramuscular, intravenous), transdermal, and like forms of administration are
possible, but
oral administration is preferred. Oral dosage forms include tablets, troches,
dispersions,
suspensions, solutions, capsules, and the like.
The pharmaceutical compositions of the present invention comprise
hydroxylansoprazole as the active ingredient, or a pharmaceutically acceptable
salt thereof,
and may also contain a pharmaceutically acceptable carrier, and optionally,
other
therapeutic ingredients. The terms "pharmaceutically acceptable salts" or "a
pharmaceutically acceptable salt thereof' refer to salts prepared from
pharmaceutically
acceptable non-toxic bases. Since the compound of the present invention is a
weak acid and
is unstable at low pH, salts may be prepared from pharmaceutically acceptable
non-toxic
bases including inorganic and organic bases. Suitable pharmaceutically
acceptable base
addition salts for the compound of the present invention include metallic
salts of aluminum,
calcium, lithium, magnesium, potassium, sodium, titanium and zinc or organic
salts made
from lysine, N,N'- dibenzylethylenediamine, chloroprocaine, choline,
diethanolamine,
ethylenediamine, meglumine (N-methylglucamine) and procaine. Sodium salts are
preferred.
The compositions of the present invention include suspensions, solutions,
elixirs or
solid dosage forms. Carriers such as starches, sugars, and microcrystalline
cellulose,
diluents, granulating agents, lubricants, binders, disintegrating agents, and
the like are
suitable in the case of oral solid preparations (such as powders, capsules,
and tablets), and
oral solid preparations are preferred over the oral liquid preparations. It
has been found that
the inclusion of mannitol and of basic salts of calcium and magnesium in the
compositions
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allows the preparation of tablets and capsules that retain good stability.
Because of the acid
instability of hydroxylansoprazole, it is usually advantageous to coat oral
solid dosage
forms with an enteric or delayed-release coating. This may be accomplished by
standard
aqueous or nonaqueous techniques. Oral dosage forms suitable for
hydroxylansoprazole are
described in US patents 5,035,899 and 5,045,321 and in PCT applications
W096/01624,
W097/12580 and W097/25030.
In addition to the common dosage forms set out above, the compounds of the
present invention may also be administered by sustained or controlled release
formulations,
which are well known in the art. Compositions suitable for rectal
administration are
described in European Application 645140.
Pharmaceutical compositions of the present invention suitable for oral
administration may be presented as discrete units such as capsules, cachets,
or tablets, each
containing a predetermined amount of the active ingredient, as a powder or
granules, or as a
solution or a suspension in an aqueous liquid, a non-aqueous liquid, an oil-in-
water
emulsion, or a water-in-oil liquid emulsion. Such compositions may be prepared
by any of
the methods of pharmacy, but all methods include the step of bringing into
association the
active ingredient with the carrier which constitutes one or more necessary
ingredients. In
general, the compositions are prepared by uniformly and intimately admixing
the active
ingredient with liquid carriers or finely divided solid carriers or both, and
then, if necessary,
shaping the product into the desired presentation.
For example, a tablet may be prepared by compression or molding, optionally,
with
one or more accessory ingredients. Compressed tablets may be prepared by
compressing in
a suitable machine the active ingredient in a free-flowing form such as powder
or granules,
optionally mixed with a binder, lubricant, inert diluent, surface active agent
or dispersing
agent. Molded tablets may be made by molding in a suitable machine, a mixture
of the
powdered compound moistened with an inert liquid diluent. Desirably, each
tablet or
capsule contains about 100-500 mg of the active ingredient.
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An enteric coating, such as the polyacrylate Eudragit L and Eudragit S
series, is
applied, preferably with an aqueous dispersion of the coating polymer. Tablets
of other
strengths may be prepared by altering the ratio of active ingredient to the
excipients or to the
final weight of the tablet.
The invention is further defined by reference to the following examples
describing in
detail the preparation of the compositions of the present invention. It will
be apparent to
those skilled in the art that many modifications, both to materials and
methods, may be
practiced without departing from the invention.
EXAMPLES
Example 1- 250 mg Tablets
Composition per tablet:
hydroxylansoprazole 250 mg
croscarmellose 60 mg
colloidal silicon dioxide 8 mg
magnesium stearate 1 mg
microcrystalline cellulose 190 mg
croscarmellose 15 mg
talc 10 mg
Total 534 mg
EXAMPLE 1
Hydroxylansoprazole and silicon dioxide are dry mixed, the first portion of
croscarmellose is added and the mixture is further dry mixed. The magnesium
stearate is
added, dry mixed and the mixture is run through a roller compactor and niill.
The resulting
dry granulate is mixed with the remaining three ingredients and compressed
into tablets.
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Example 2 - 200 mg Tablets
Composition per unit dosage:
hydroxylansoprazole 200 mg
pregelatinized starch 200 mg
microcrystalline cellulose 25 mg
povidone 15 mg
croscarmellose 10 mg
magnesium stearate 3.75 mg
FD&C yellow #21ake 2.5 mg
Water (5 mL)
Total 456.25 mg
EXAMPLE 2
The ingredients above are mixed well in the proportions shown in a high shear
mixer until uniform granules result. The nzixture is tray-dried at 40 C
under vacuum until
the desired consistency is reached. The granules are milled to less than 60
mesh using a
screen mill and compressed into tablets.
Example 3 - Enteric Coating
Enteric coating
composition:
Eudragit L-30D 138 mg (solids 41.4 mg)
Talc 4.1 mg
Polyethylene glycol
5000 12.4 mg
TweenTM 80 2.1 mg
Water 250 l
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Enteric tablets are produced in a pan coater by coating the tablets obtained
in
Example 2 with the enteric coating composition shown.
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